LOC108856492 cDNA ORF clone, Raphanus sativus(radish)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC108856492
Entrez Gene ID	108856492
Full Name	tRNA (guanine(37)-N1)-methyltransferase 1
Gene Type	protein-coding
Organism	Raphanus sativus(radish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_018630302.1	XP_018485804.1	tRNA (guanine(37)-N1)-methyltransferase 1

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

De novo transcriptome analysis in radish (Raphanus sativus L.) and identification of critical genes involved in bolting and flowering.
Nie S, Li C, Xu L, Wang Y, Huang D, Muleke EM, Sun X, Xie Y, Liu L
BMC genomics17389(2016 05)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following LOC108856492 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC108856492 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORc19387	XM_018630302.1 Latest version!	Raphanus sativus tRNA (guanine(37)-N1)-methyltransferase 1 (LOC108856492), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$258.30 $369.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC108856492 ( XM_018630302.1 ) cDNA ORF clone, Raphanus sativus(radish) -> XP_018485804.1 Raphanus sativus tRNA (guanine(37)-N1)-methyltransferase 1 (LOC108856492), mRNA.

Price & Availability↑

CloneID	ORc19387
Clone ID Related Accession (Same CDS sequence)	XM_018630302.1
Accession Version	XM_018630302.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1344bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-10-03
Organism	Raphanus sativus(radish)
Product	tRNA (guanine(37)-N1)-methyltransferase 1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_017353143.1) and transcript sequence (GEMG01042540.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Raphanus sativus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## assembly gap :: added 15 transcript bases to patch genome assembly gap ##RefSeq-Attributes-END##

ATGTTGGATG AAAGCAACTT TGATGTCAAT CTGAAGCTGT GGGCTATTCG GATTCCCCGT 
GAACTCTGTA AAAGCGCAAC TAAGATACTC AATGGATACA TGCTCAACAT GCCTCGGATT 
AAACCCATCA CTGAAGAATC CGGTTTTGAA AAGACTCGTT TGGTGTTACT GTCGGAAACT 
GTTAAAAGTG CCGATTTGTC GGAGATACCA GATGAAAAAC TAAACCAGCT TAAGAAACTA 
AGTGAAGTTG AAGTAGTTCC TCACTCGGTC ACCCTTGGCT ATTCCTATTG GAGTGCAGAT 
CACATATTGA AGCAGATTCT GCCAGAGGGA GTGGATATAC CTTCATCTTT TGAAACCATA 
GGTCATATAG CCCACTTAAA TCTACACGAC GAGCTCCTTC CCTTCAAAGA CGTGATAGCT 
AAGGTTATCT ATGATAAAAA CTATCCAAGG ATCAAGACAA TTGTGAACAA AGTCGGAACC 
ATCTCCAACG AGTTTCGAGT GCCAAAGTTC GAAGTGTTAG CCGGAGAAAC TGGGATGGAA 
ACAGAAGTAA AGCAATACGG AGCAAGGTTT AAGCTGGACT ACAGCCTTGT TTACTGGAAC 
TCGAGACTGG AGCATGAGCA CATGCGTCTT ACGTCTCTGT TTAAACCTGG AGAAACCGTG 
TGTGATATGT TTGCTGGTAT TGGTCCTTTT GCTATTCCAG CTGCGCAAAA GGGATGCTTC 
GTTTATGCGA ATGATTTGAA TCCGGACAGT GTTCGGTACT TGAAGATTAA TGCAGAGTTC 
AACAAGGTTG ATGAGTTGAT CTGTGTGCAC AATATGGATG CTAGAAAATT TATTTCTCAG 
TTGATGAAGG TCTCAGATGG TGAGGGTAAT CTGCAATCGG TTGCTGATGA CAAAACTAAG 
GAAGCAGTGG CAAGTCAAGA AGGTAGTGCA ATTAAGAAGC CATCTGGTGT TTCAGAGACA 
GAAAATGGGG TAGGGAAAGA CTGCAAGACT ATAGAAGGAA ACGCAAAGAA GAGGTTGAAA 
CAAACGGTGC TTCCAATCGC AAAGCCGTGG GAACATGTCG ACCATGTTAT TATGAACCTT 
CCAGCTTCTG CCTTGCAGTT TCTCGATTCT TTCAGTAAAG TGATTGAAAA GAAGTATTGG 
AAAGGACCTC TTCCTTTAAT CCATTGCTAC TGTTTCATTC GTGCAAGCGA AACCACAGAG 
TCTATTATTG CTGAAGCTGA AAGTGCTTTG AAGTTCCATA TAGAAGATCC GGTCTTCCAC 
AAGGTCAGAG ATGTTGCTCC AAACAAGGCG ATGTTTTGCC TAAGCTTTAG ACTGCCTGAA 
GCATGCTTGA TGGAGGAACA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_018485804.1
CDS	99..1442
Translation	MLDESNFDVNLKLWAIRIPRELCKSATKILNGYMLNMPRIKPITEESGFEKTRLVLLSETVKSADLSEIPDEKLNQLKKLSEVEVVPHSVTLGYSYWSADHILKQILPEGVDIPSSFETIGHIAHLNLHDELLPFKDVIAKVIYDKNYPRIKTIVNKVGTISNEFRVPKFEVLAGETGMETEVKQYGARFKLDYSLVYWNSRLEHEHMRLTSLFKPGETVCDMFAGIGPFAIPAAQKGCFVYANDLNPDSVRYLKINAEFNKVDELICVHNMDARKFISQLMKVSDGEGNLQSVADDKTKEAVASQEGSAIKKPSGVSETENGVGKDCKTIEGNAKKRLKQTVLPIAKPWEHVDHVIMNLPASALQFLDSFSKVIEKKYWKGPLPLIHCYCFIRASETTESIIAEAESALKFHIEDPVFHKVRDVAPNKAMFCLSFRLPEACLMEEH

Target ORF information:

RefSeq Version	XM_018630302.1
Organism	Raphanus sativus(radish)
Definition	Raphanus sativus tRNA (guanine(37)-N1)-methyltransferase 1 (LOC108856492), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018630302.1

ORF Insert Sequence:

ATGTTGGATG AAAGCAACTT TGATGTCAAT CTGAAGCTGT GGGCTATTCG GATTCCCCGT 
GAACTCTGTA AAAGCGCAAC TAAGATACTC AATGGATACA TGCTCAACAT GCCTCGGATT 
AAACCCATCA CTGAAGAATC CGGTTTTGAA AAGACTCGTT TGGTGTTACT GTCGGAAACT 
GTTAAAAGTG CCGATTTGTC GGAGATACCA GATGAAAAAC TAAACCAGCT TAAGAAACTA 
AGTGAAGTTG AAGTAGTTCC TCACTCGGTC ACCCTTGGCT ATTCCTATTG GAGTGCAGAT 
CACATATTGA AGCAGATTCT GCCAGAGGGA GTGGATATAC CTTCATCTTT TGAAACCATA 
GGTCATATAG CCCACTTAAA TCTACACGAC GAGCTCCTTC CCTTCAAAGA CGTGATAGCT 
AAGGTTATCT ATGATAAAAA CTATCCAAGG ATCAAGACAA TTGTGAACAA AGTCGGAACC 
ATCTCCAACG AGTTTCGAGT GCCAAAGTTC GAAGTGTTAG CCGGAGAAAC TGGGATGGAA 
ACAGAAGTAA AGCAATACGG AGCAAGGTTT AAGCTGGACT ACAGCCTTGT TTACTGGAAC 
TCGAGACTGG AGCATGAGCA CATGCGTCTT ACGTCTCTGT TTAAACCTGG AGAAACCGTG 
TGTGATATGT TTGCTGGTAT TGGTCCTTTT GCTATTCCAG CTGCGCAAAA GGGATGCTTC 
GTTTATGCGA ATGATTTGAA TCCGGACAGT GTTCGGTACT TGAAGATTAA TGCAGAGTTC 
AACAAGGTTG ATGAGTTGAT CTGTGTGCAC AATATGGATG CTAGAAAATT TATTTCTCAG 
TTGATGAAGG TCTCAGATGG TGAGGGTAAT CTGCAATCGG TTGCTGATGA CAAAACTAAG 
GAAGCAGTGG CAAGTCAAGA AGGTAGTGCA ATTAAGAAGC CATCTGGTGT TTCAGAGACA 
GAAAATGGGG TAGGGAAAGA CTGCAAGACT ATAGAAGGAA ACGCAAAGAA GAGGTTGAAA 
CAAACGGTGC TTCCAATCGC AAAGCCGTGG GAACATGTCG ACCATGTTAT TATGAACCTT 
CCAGCTTCTG CCTTGCAGTT TCTCGATTCT TTCAGTAAAG TGATTGAAAA GAAGTATTGG 
AAAGGACCTC TTCCTTTAAT CCATTGCTAC TGTTTCATTC GTGCAAGCGA AACCACAGAG 
TCTATTATTG CTGAAGCTGA AAGTGCTTTG AAGTTCCATA TAGAAGATCC GGTCTTCCAC 
AAGGTCAGAG ATGTTGCTCC AAACAAGGCG ATGTTTTGCC TAAGCTTTAG ACTGCCTGAA 
GCATGCTTGA TGGAGGAACA CTGA