ide cDNA ORF clone, Pundamilia nyererei

Gene
Clones
gRNAs

Summary

Gene Symbol	ide
Entrez Gene ID	102204010
Full Name	insulin degrading enzyme
Gene Type	protein-coding
Organism	Pundamilia nyererei

mRNA and Protein(s)

mRNA	Protein	Name
XM_005735095.1	XP_005735152.1	insulin-degrading enzyme isoform X2
XM_005735094.2	XP_005735151.1	insulin-degrading enzyme isoform X1

Pathways from BioSystems
Gene Ontology

The following ide gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ide cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OPu65487	XM_005735094.2 Latest version!	Pundamilia nyererei insulin-degrading enzyme (ide), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
OPu65488	XM_005735095.1 Latest version!	Pundamilia nyererei insulin-degrading enzyme (ide), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ide ( XM_005735094.2 ) cDNA ORF clone, Pundamilia nyererei -> XP_005735151.1 Pundamilia nyererei insulin-degrading enzyme (ide), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OPu65487
Clone ID Related Accession (Same CDS sequence)	XM_005735094.2
Accession Version	XM_005735094.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3000bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-08-30
Organism	Pundamilia nyererei
Product	insulin-degrading enzyme isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005187463.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Aug 31, 2015 this sequence version replaced XM_005735094.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pundamilia nyererei Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTTCCCG TCTGTTTCAC CTTCAACTAT ACCAGGATGC TGGTGTCATC CTTGTCACTC 
AGAATGACCG GCCAGGTTGT GAGGAGGGTA GTTAGTGATG TCATTCGCTC ACCTGAGGAC 
AAGCGGGAGT ACAGGGGCCT GGAGTTTACC AATGGCCTGA AGGCAATGCT CATCAGTGAC 
CCGACCACAG ACAAATCTTC AGCTGCTTTG GATGTCCATA TAGGTTCTCT GTCTGACCCA 
CCAAACATCT CAGGGCTGGC ACACTTTTGT GAACACATGC TGTTCCTGGG AACAGAGAAG 
TACCCCAAGG AGAACGAGTA CAGCCAGTTC CTCAGTGAGC ACGCAGGCAG CTCCAATGCC 
TTCACCAGCA GAGAACATAC CAACTATTAC TTTGATGTTT CCCATGAGCA CTTGAAAGGA 
GCATTAGATA GGTTTGCCCA GTTCTTTCTG TGCCCATTGT TTGACGAGAG CTGCAAGGAC 
CGCGAGGTGA ACGCTGTGGA CTCTGAGTAC GAGAAGAACC TGAAGAATGA TACCTGGAGG 
CTGTTTCAGT TGGAAAAGGC CACTGGCAAC CCAAAACACC CTTTCAGTAA ATTTGGAACA 
GGTAATAGAA TGACACTAGA GACAAGACCA TCTAAAGAGG GGATTGATAT CCGTAAGGAG 
CTCCTGAAGT TCCACTCCAC GTATTACTCA TCCAACCTCA TGGGACTGTG TGTGTTAGGA 
AGAGAGTCGT TGGATGAGTT GACGGCCATG GTGGTTAAGT TATTTGGAGA AGTGGAAAAC 
AAGAATGTGC CTATTCCAGA ATTCCCTGAA CACCCTTTAC AAGAAGAACA CCTTAAAAAA 
TTCTACAAAG TGGTTCCTGT CAAAGACATT AGGAAACTTT ATGTGACTTT CCCCATCCCA 
GATCTACAGC AGTACTACAA ATCGAAGCCA GGACGTTATC TGGGACATCT GATTGGTCAC 
GAAGGACCTG GAAGTTTGTT TTCTGAGCTA AAATCTAAAG GCTGGGTGGA CACAATCCTT 
GGAGGACACA AAGAGGGAGC CAGAGGATTC ATGTTCTTCA ACATCAAGAT GGACTTGACT 
GAGGAGGGCC TCTTGAACAT TGAGGACATA ATCTTCCACA TGTTCCAGTA CATCCAGAAA 
CTCCGTTCTG AAGGGCCTCA AGAGTGGGTG TTTAACGAGT ACAAGGATTT AAAAAAAGTT 
GCTTTCAGGT TTAAAGACAA GGAGCGCCCT CGTGACTACA CTTCCAAAAT AGCAGGTTTA 
CTACAGCACT ACCCTCTTGA AGAAGTTCTA TCAGCAGAGT ACTTCTTGGA AGACTTCAAG 
CCAGATCTGA TCGAGATGGT GCTGGATAAG CTGAGACCAG AACACGTCAG AGTTATAGTT 
GTGTCAAAAT CATTTGAAGG ACAAACAGAC ATAACAGAAG AGTGGTACGG CACGCAGTAC 
AAACAGGAGG CCATCTCTGA GGAGACCATC AAGAAATGGG CAAATGCAGA CCTCAACGGC 
AAATTCAAAT TGCCAATGAA AAATGAGTTC ATCCCCACCA ACTTCGAGAT CTACCCTCTT 
GAGAAAGACT CTCCGTCAGT TCCCACTTTA ATCAAGGACA CTGCTATGAG CAAGGTGTGG 
TTCAAGCAGG ATGACAAATT CTTCCTCCCC AAGGCTTGCC TGAACTTTGA GTTCTTCAGC 
CCGTTTGCGT ACGTGGACCC ATTGCATTGT AACATGGCAT ATTTATACCT GGAGCTCCTC 
AAGGACTCCC TCAACGAGTA TGCATATGCA GCCGAGCTAG CTGGCTTGAA CTATGAACTC 
CAAAATACCG CCTATGGAAT GCATCTGTCT GTTAAAGGCT ACAGTGACAA ACAGAACATC 
CTGCTGAAGA AGATCATCGA GAAGATGGCC ACTTTTGAGA TAGACGAGAA GCGCTTTGAC 
ATCATCAAGG AAGCTTACAT GAGGTCTTTG AATAACTTCA GAGCTGAGAG GCCTAACTAT 
CATGCTATGT ACTACCTCCA TCTTCTAATG ACCGAGGTTG TTTGGACCAA AGATGAGCTC 
AGAGAGGCTC TAGATGATGT AACTCTCCCA CGCCTTAACA TCTTCATACC TCAGCTGTTG 
TCGCGGTTAC ATATCAAAGT CCTTCTTCAT GGCAACATCA CCAAGGAGTC TGCTCTTGGC 
ATGATGCAAA TGGTGGAGGA CACGCTCACT GAGCATGCTC ACACAAAGCC TCTCCTCCCG 
AGCCAGCTGA TTCGCTACAG ACAGGTGCAG GTTCCAGACG GTGGCTGGTA TGTTTACCAG 
CAAAAGAACG CGGTGCACAA TGATTGTGGC ATCGATATCT ACTACCAAAT AGACATGCAG 
AGTACACACG ACAACATGCT GCTGGAGCTG TTCGCTCAGA TCATCTCTGA GCCCTGCTAT 
AGTACACTGA GAACCAAAGA ACAGCTGGGC TACAGCGTGC ATAGTGGGCC ACGTCGGGCT 
AACGGAGTGC AAGGTCTCTA CTTCCTCATC CAGTCGGAGA AGGCGCCCCA CTACCTGGAG 
AGCCGAGTGG AAGCCTTCCT GTGTACTATG GAGAAAGCTG TGGAGGAGAT GAGCGAGGAA 
GCCTTCCAGA AACACATCCA AGCCCTGGCC ATCCGCCGCC TGGACAAACC GAAGAAGCTG 
TCTGCTGAGT GTGCTAAATA CTGGGGAGAG ATCATCTCTC AGCAGTACAA TTTTGATAGA 
GACAACATTG AAGTGGCGTA TCTGAAGACT CTGACAAAAG AAAACATAAT GGAGTTTTAC 
AGAGAGCGAC TGACGGTCGA AGCCCCAAAG AGACACAAAG TGTCGGTGCA CGTGCTGTCC 
AGGGAGATGG ACACCTGTCC AGTAGTGGGA GAGTTCCCCG CTCAGAATGA TGTCAACCTG 
GCTCCTGCTC CTTCCCTGCC ACAGCCCACA GTGATTCAGG ACATGATGGA GTTCAAGAGG 
AGTCTGCCTC TGTTCCCTCT GGTCAAACCT CACATCAACC TCAGGGCAGC CAAACTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005735151.1
CDS	75..3074
Translation	MLPVCFTFNYTRMLVSSLSLRMTGQVVRRVVSDVIRSPEDKREYRGLEFTNGLKAMLISDPTTDKSSAALDVHIGSLSDPPNISGLAHFCEHMLFLGTEKYPKENEYSQFLSEHAGSSNAFTSREHTNYYFDVSHEHLKGALDRFAQFFLCPLFDESCKDREVNAVDSEYEKNLKNDTWRLFQLEKATGNPKHPFSKFGTGNRMTLETRPSKEGIDIRKELLKFHSTYYSSNLMGLCVLGRESLDELTAMVVKLFGEVENKNVPIPEFPEHPLQEEHLKKFYKVVPVKDIRKLYVTFPIPDLQQYYKSKPGRYLGHLIGHEGPGSLFSELKSKGWVDTILGGHKEGARGFMFFNIKMDLTEEGLLNIEDIIFHMFQYIQKLRSEGPQEWVFNEYKDLKKVAFRFKDKERPRDYTSKIAGLLQHYPLEEVLSAEYFLEDFKPDLIEMVLDKLRPEHVRVIVVSKSFEGQTDITEEWYGTQYKQEAISEETIKKWANADLNGKFKLPMKNEFIPTNFEIYPLEKDSPSVPTLIKDTAMSKVWFKQDDKFFLPKACLNFEFFSPFAYVDPLHCNMAYLYLELLKDSLNEYAYAAELAGLNYELQNTAYGMHLSVKGYSDKQNILLKKIIEKMATFEIDEKRFDIIKEAYMRSLNNFRAERPNYHAMYYLHLLMTEVVWTKDELREALDDVTLPRLNIFIPQLLSRLHIKVLLHGNITKESALGMMQMVEDTLTEHAHTKPLLPSQLIRYRQVQVPDGGWYVYQQKNAVHNDCGIDIYYQIDMQSTHDNMLLELFAQIISEPCYSTLRTKEQLGYSVHSGPRRANGVQGLYFLIQSEKAPHYLESRVEAFLCTMEKAVEEMSEEAFQKHIQALAIRRLDKPKKLSAECAKYWGEIISQQYNFDRDNIEVAYLKTLTKENIMEFYRERLTVEAPKRHKVSVHVLSREMDTCPVVGEFPAQNDVNLAPAPSLPQPTVIQDMMEFKRSLPLFPLVKPHINLRAAKL

Target ORF information:

RefSeq Version	XM_005735094.2
Organism	Pundamilia nyererei
Definition	Pundamilia nyererei insulin-degrading enzyme (ide), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005735094.2

ORF Insert Sequence:

ATGCTTCCCG TCTGTTTCAC CTTCAACTAT ACCAGGATGC TGGTGTCATC CTTGTCACTC 
AGAATGACCG GCCAGGTTGT GAGGAGGGTA GTTAGTGATG TCATTCGCTC ACCTGAGGAC 
AAGCGGGAGT ACAGGGGCCT GGAGTTTACC AATGGCCTGA AGGCAATGCT CATCAGTGAC 
CCGACCACAG ACAAATCTTC AGCTGCTTTG GATGTCCATA TAGGTTCTCT GTCTGACCCA 
CCAAACATCT CAGGGCTGGC ACACTTTTGT GAACACATGC TGTTCCTGGG AACAGAGAAG 
TACCCCAAGG AGAACGAGTA CAGCCAGTTC CTCAGTGAGC ACGCAGGCAG CTCCAATGCC 
TTCACCAGCA GAGAACATAC CAACTATTAC TTTGATGTTT CCCATGAGCA CTTGAAAGGA 
GCATTAGATA GGTTTGCCCA GTTCTTTCTG TGCCCATTGT TTGACGAGAG CTGCAAGGAC 
CGCGAGGTGA ACGCTGTGGA CTCTGAGTAC GAGAAGAACC TGAAGAATGA TACCTGGAGG 
CTGTTTCAGT TGGAAAAGGC CACTGGCAAC CCAAAACACC CTTTCAGTAA ATTTGGAACA 
GGTAATAGAA TGACACTAGA GACAAGACCA TCTAAAGAGG GGATTGATAT CCGTAAGGAG 
CTCCTGAAGT TCCACTCCAC GTATTACTCA TCCAACCTCA TGGGACTGTG TGTGTTAGGA 
AGAGAGTCGT TGGATGAGTT GACGGCCATG GTGGTTAAGT TATTTGGAGA AGTGGAAAAC 
AAGAATGTGC CTATTCCAGA ATTCCCTGAA CACCCTTTAC AAGAAGAACA CCTTAAAAAA 
TTCTACAAAG TGGTTCCTGT CAAAGACATT AGGAAACTTT ATGTGACTTT CCCCATCCCA 
GATCTACAGC AGTACTACAA ATCGAAGCCA GGACGTTATC TGGGACATCT GATTGGTCAC 
GAAGGACCTG GAAGTTTGTT TTCTGAGCTA AAATCTAAAG GCTGGGTGGA CACAATCCTT 
GGAGGACACA AAGAGGGAGC CAGAGGATTC ATGTTCTTCA ACATCAAGAT GGACTTGACT 
GAGGAGGGCC TCTTGAACAT TGAGGACATA ATCTTCCACA TGTTCCAGTA CATCCAGAAA 
CTCCGTTCTG AAGGGCCTCA AGAGTGGGTG TTTAACGAGT ACAAGGATTT AAAAAAAGTT 
GCTTTCAGGT TTAAAGACAA GGAGCGCCCT CGTGACTACA CTTCCAAAAT AGCAGGTTTA 
CTACAGCACT ACCCTCTTGA AGAAGTTCTA TCAGCAGAGT ACTTCTTGGA AGACTTCAAG 
CCAGATCTGA TCGAGATGGT GCTGGATAAG CTGAGACCAG AACACGTCAG AGTTATAGTT 
GTGTCAAAAT CATTTGAAGG ACAAACAGAC ATAACAGAAG AGTGGTACGG CACGCAGTAC 
AAACAGGAGG CCATCTCTGA GGAGACCATC AAGAAATGGG CAAATGCAGA CCTCAACGGC 
AAATTCAAAT TGCCAATGAA AAATGAGTTC ATCCCCACCA ACTTCGAGAT CTACCCTCTT 
GAGAAAGACT CTCCGTCAGT TCCCACTTTA ATCAAGGACA CTGCTATGAG CAAGGTGTGG 
TTCAAGCAGG ATGACAAATT CTTCCTCCCC AAGGCTTGCC TGAACTTTGA GTTCTTCAGC 
CCGTTTGCGT ACGTGGACCC ATTGCATTGT AACATGGCAT ATTTATACCT GGAGCTCCTC 
AAGGACTCCC TCAACGAGTA TGCATATGCA GCCGAGCTAG CTGGCTTGAA CTATGAACTC 
CAAAATACCG CCTATGGAAT GCATCTGTCT GTTAAAGGCT ACAGTGACAA ACAGAACATC 
CTGCTGAAGA AGATCATCGA GAAGATGGCC ACTTTTGAGA TAGACGAGAA GCGCTTTGAC 
ATCATCAAGG AAGCTTACAT GAGGTCTTTG AATAACTTCA GAGCTGAGAG GCCTAACTAT 
CATGCTATGT ACTACCTCCA TCTTCTAATG ACCGAGGTTG TTTGGACCAA AGATGAGCTC 
AGAGAGGCTC TAGATGATGT AACTCTCCCA CGCCTTAACA TCTTCATACC TCAGCTGTTG 
TCGCGGTTAC ATATCAAAGT CCTTCTTCAT GGCAACATCA CCAAGGAGTC TGCTCTTGGC 
ATGATGCAAA TGGTGGAGGA CACGCTCACT GAGCATGCTC ACACAAAGCC TCTCCTCCCG 
AGCCAGCTGA TTCGCTACAG ACAGGTGCAG GTTCCAGACG GTGGCTGGTA TGTTTACCAG 
CAAAAGAACG CGGTGCACAA TGATTGTGGC ATCGATATCT ACTACCAAAT AGACATGCAG 
AGTACACACG ACAACATGCT GCTGGAGCTG TTCGCTCAGA TCATCTCTGA GCCCTGCTAT 
AGTACACTGA GAACCAAAGA ACAGCTGGGC TACAGCGTGC ATAGTGGGCC ACGTCGGGCT 
AACGGAGTGC AAGGTCTCTA CTTCCTCATC CAGTCGGAGA AGGCGCCCCA CTACCTGGAG 
AGCCGAGTGG AAGCCTTCCT GTGTACTATG GAGAAAGCTG TGGAGGAGAT GAGCGAGGAA 
GCCTTCCAGA AACACATCCA AGCCCTGGCC ATCCGCCGCC TGGACAAACC GAAGAAGCTG 
TCTGCTGAGT GTGCTAAATA CTGGGGAGAG ATCATCTCTC AGCAGTACAA TTTTGATAGA 
GACAACATTG AAGTGGCGTA TCTGAAGACT CTGACAAAAG AAAACATAAT GGAGTTTTAC 
AGAGAGCGAC TGACGGTCGA AGCCCCAAAG AGACACAAAG TGTCGGTGCA CGTGCTGTCC 
AGGGAGATGG ACACCTGTCC AGTAGTGGGA GAGTTCCCCG CTCAGAATGA TGTCAACCTG 
GCTCCTGCTC CTTCCCTGCC ACAGCCCACA GTGATTCAGG ACATGATGGA GTTCAAGAGG 
AGTCTGCCTC TGTTCCCTCT GGTCAAACCT CACATCAACC TCAGGGCAGC CAAACTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ide ( XM_005735095.1 ) cDNA ORF clone, Pundamilia nyererei -> XP_005735152.1 Pundamilia nyererei insulin-degrading enzyme (ide), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OPu65488
Clone ID Related Accession (Same CDS sequence)	XM_005735095.1
Accession Version	XM_005735095.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2937bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-08-30
Organism	Pundamilia nyererei
Product	insulin-degrading enzyme isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005187463.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pundamilia nyererei Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCGGCC AGGTTGTGAG GAGGGTAGTT AGTGATGTCA TTCGCTCACC TGAGGACAAG 
CGGGAGTACA GGGGCCTGGA GTTTACCAAT GGCCTGAAGG CAATGCTCAT CAGTGACCCG 
ACCACAGACA AATCTTCAGC TGCTTTGGAT GTCCATATAG GTTCTCTGTC TGACCCACCA 
AACATCTCAG GGCTGGCACA CTTTTGTGAA CACATGCTGT TCCTGGGAAC AGAGAAGTAC 
CCCAAGGAGA ACGAGTACAG CCAGTTCCTC AGTGAGCACG CAGGCAGCTC CAATGCCTTC 
ACCAGCAGAG AACATACCAA CTATTACTTT GATGTTTCCC ATGAGCACTT GAAAGGAGCA 
TTAGATAGGT TTGCCCAGTT CTTTCTGTGC CCATTGTTTG ACGAGAGCTG CAAGGACCGC 
GAGGTGAACG CTGTGGACTC TGAGTACGAG AAGAACCTGA AGAATGATAC CTGGAGGCTG 
TTTCAGTTGG AAAAGGCCAC TGGCAACCCA AAACACCCTT TCAGTAAATT TGGAACAGGT 
AATAGAATGA CACTAGAGAC AAGACCATCT AAAGAGGGGA TTGATATCCG TAAGGAGCTC 
CTGAAGTTCC ACTCCACGTA TTACTCATCC AACCTCATGG GACTGTGTGT GTTAGGAAGA 
GAGTCGTTGG ATGAGTTGAC GGCCATGGTG GTTAAGTTAT TTGGAGAAGT GGAAAACAAG 
AATGTGCCTA TTCCAGAATT CCCTGAACAC CCTTTACAAG AAGAACACCT TAAAAAATTC 
TACAAAGTGG TTCCTGTCAA AGACATTAGG AAACTTTATG TGACTTTCCC CATCCCAGAT 
CTACAGCAGT ACTACAAATC GAAGCCAGGA CGTTATCTGG GACATCTGAT TGGTCACGAA 
GGACCTGGAA GTTTGTTTTC TGAGCTAAAA TCTAAAGGCT GGGTGGACAC AATCCTTGGA 
GGACACAAAG AGGGAGCCAG AGGATTCATG TTCTTCAACA TCAAGATGGA CTTGACTGAG 
GAGGGCCTCT TGAACATTGA GGACATAATC TTCCACATGT TCCAGTACAT CCAGAAACTC 
CGTTCTGAAG GGCCTCAAGA GTGGGTGTTT AACGAGTACA AGGATTTAAA AAAAGTTGCT 
TTCAGGTTTA AAGACAAGGA GCGCCCTCGT GACTACACTT CCAAAATAGC AGGTTTACTA 
CAGCACTACC CTCTTGAAGA AGTTCTATCA GCAGAGTACT TCTTGGAAGA CTTCAAGCCA 
GATCTGATCG AGATGGTGCT GGATAAGCTG AGACCAGAAC ACGTCAGAGT TATAGTTGTG 
TCAAAATCAT TTGAAGGACA AACAGACATA ACAGAAGAGT GGTACGGCAC GCAGTACAAA 
CAGGAGGCCA TCTCTGAGGA GACCATCAAG AAATGGGCAA ATGCAGACCT CAACGGCAAA 
TTCAAATTGC CAATGAAAAA TGAGTTCATC CCCACCAACT TCGAGATCTA CCCTCTTGAG 
AAAGACTCTC CGTCAGTTCC CACTTTAATC AAGGACACTG CTATGAGCAA GGTGTGGTTC 
AAGCAGGATG ACAAATTCTT CCTCCCCAAG GCTTGCCTGA ACTTTGAGTT CTTCAGCCCG 
TTTGCGTACG TGGACCCATT GCATTGTAAC ATGGCATATT TATACCTGGA GCTCCTCAAG 
GACTCCCTCA ACGAGTATGC ATATGCAGCC GAGCTAGCTG GCTTGAACTA TGAACTCCAA 
AATACCGCCT ATGGAATGCA TCTGTCTGTT AAAGGCTACA GTGACAAACA GAACATCCTG 
CTGAAGAAGA TCATCGAGAA GATGGCCACT TTTGAGATAG ACGAGAAGCG CTTTGACATC 
ATCAAGGAAG CTTACATGAG GTCTTTGAAT AACTTCAGAG CTGAGAGGCC TAACTATCAT 
GCTATGTACT ACCTCCATCT TCTAATGACC GAGGTTGTTT GGACCAAAGA TGAGCTCAGA 
GAGGCTCTAG ATGATGTAAC TCTCCCACGC CTTAACATCT TCATACCTCA GCTGTTGTCG 
CGGTTACATA TCAAAGTCCT TCTTCATGGC AACATCACCA AGGAGTCTGC TCTTGGCATG 
ATGCAAATGG TGGAGGACAC GCTCACTGAG CATGCTCACA CAAAGCCTCT CCTCCCGAGC 
CAGCTGATTC GCTACAGACA GGTGCAGGTT CCAGACGGTG GCTGGTATGT TTACCAGCAA 
AAGAACGCGG TGCACAATGA TTGTGGCATC GATATCTACT ACCAAATAGA CATGCAGAGT 
ACACACGACA ACATGCTGCT GGAGCTGTTC GCTCAGATCA TCTCTGAGCC CTGCTATAGT 
ACACTGAGAA CCAAAGAACA GCTGGGCTAC AGCGTGCATA GTGGGCCACG TCGGGCTAAC 
GGAGTGCAAG GTCTCTACTT CCTCATCCAG TCGGAGAAGG CGCCCCACTA CCTGGAGAGC 
CGAGTGGAAG CCTTCCTGTG TACTATGGAG AAAGCTGTGG AGGAGATGAG CGAGGAAGCC 
TTCCAGAAAC ACATCCAAGC CCTGGCCATC CGCCGCCTGG ACAAACCGAA GAAGCTGTCT 
GCTGAGTGTG CTAAATACTG GGGAGAGATC ATCTCTCAGC AGTACAATTT TGATAGAGAC 
AACATTGAAG TGGCGTATCT GAAGACTCTG ACAAAAGAAA ACATAATGGA GTTTTACAGA 
GAGCGACTGA CGGTCGAAGC CCCAAAGAGA CACAAAGTGT CGGTGCACGT GCTGTCCAGG 
GAGATGGACA CCTGTCCAGT AGTGGGAGAG TTCCCCGCTC AGAATGATGT CAACCTGGCT 
CCTGCTCCTT CCCTGCCACA GCCCACAGTG ATTCAGGACA TGATGGAGTT CAAGAGGAGT 
CTGCCTCTGT TCCCTCTGGT CAAACCTCAC ATCAACCTCA GGGCAGCCAA ACTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005735152.1
CDS	227..3163
Translation	MTGQVVRRVVSDVIRSPEDKREYRGLEFTNGLKAMLISDPTTDKSSAALDVHIGSLSDPPNISGLAHFCEHMLFLGTEKYPKENEYSQFLSEHAGSSNAFTSREHTNYYFDVSHEHLKGALDRFAQFFLCPLFDESCKDREVNAVDSEYEKNLKNDTWRLFQLEKATGNPKHPFSKFGTGNRMTLETRPSKEGIDIRKELLKFHSTYYSSNLMGLCVLGRESLDELTAMVVKLFGEVENKNVPIPEFPEHPLQEEHLKKFYKVVPVKDIRKLYVTFPIPDLQQYYKSKPGRYLGHLIGHEGPGSLFSELKSKGWVDTILGGHKEGARGFMFFNIKMDLTEEGLLNIEDIIFHMFQYIQKLRSEGPQEWVFNEYKDLKKVAFRFKDKERPRDYTSKIAGLLQHYPLEEVLSAEYFLEDFKPDLIEMVLDKLRPEHVRVIVVSKSFEGQTDITEEWYGTQYKQEAISEETIKKWANADLNGKFKLPMKNEFIPTNFEIYPLEKDSPSVPTLIKDTAMSKVWFKQDDKFFLPKACLNFEFFSPFAYVDPLHCNMAYLYLELLKDSLNEYAYAAELAGLNYELQNTAYGMHLSVKGYSDKQNILLKKIIEKMATFEIDEKRFDIIKEAYMRSLNNFRAERPNYHAMYYLHLLMTEVVWTKDELREALDDVTLPRLNIFIPQLLSRLHIKVLLHGNITKESALGMMQMVEDTLTEHAHTKPLLPSQLIRYRQVQVPDGGWYVYQQKNAVHNDCGIDIYYQIDMQSTHDNMLLELFAQIISEPCYSTLRTKEQLGYSVHSGPRRANGVQGLYFLIQSEKAPHYLESRVEAFLCTMEKAVEEMSEEAFQKHIQALAIRRLDKPKKLSAECAKYWGEIISQQYNFDRDNIEVAYLKTLTKENIMEFYRERLTVEAPKRHKVSVHVLSREMDTCPVVGEFPAQNDVNLAPAPSLPQPTVIQDMMEFKRSLPLFPLVKPHINLRAAKL

Target ORF information:

RefSeq Version	XM_005735095.1
Organism	Pundamilia nyererei
Definition	Pundamilia nyererei insulin-degrading enzyme (ide), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005735095.1

ORF Insert Sequence:

ATGACCGGCC AGGTTGTGAG GAGGGTAGTT AGTGATGTCA TTCGCTCACC TGAGGACAAG 
CGGGAGTACA GGGGCCTGGA GTTTACCAAT GGCCTGAAGG CAATGCTCAT CAGTGACCCG 
ACCACAGACA AATCTTCAGC TGCTTTGGAT GTCCATATAG GTTCTCTGTC TGACCCACCA 
AACATCTCAG GGCTGGCACA CTTTTGTGAA CACATGCTGT TCCTGGGAAC AGAGAAGTAC 
CCCAAGGAGA ACGAGTACAG CCAGTTCCTC AGTGAGCACG CAGGCAGCTC CAATGCCTTC 
ACCAGCAGAG AACATACCAA CTATTACTTT GATGTTTCCC ATGAGCACTT GAAAGGAGCA 
TTAGATAGGT TTGCCCAGTT CTTTCTGTGC CCATTGTTTG ACGAGAGCTG CAAGGACCGC 
GAGGTGAACG CTGTGGACTC TGAGTACGAG AAGAACCTGA AGAATGATAC CTGGAGGCTG 
TTTCAGTTGG AAAAGGCCAC TGGCAACCCA AAACACCCTT TCAGTAAATT TGGAACAGGT 
AATAGAATGA CACTAGAGAC AAGACCATCT AAAGAGGGGA TTGATATCCG TAAGGAGCTC 
CTGAAGTTCC ACTCCACGTA TTACTCATCC AACCTCATGG GACTGTGTGT GTTAGGAAGA 
GAGTCGTTGG ATGAGTTGAC GGCCATGGTG GTTAAGTTAT TTGGAGAAGT GGAAAACAAG 
AATGTGCCTA TTCCAGAATT CCCTGAACAC CCTTTACAAG AAGAACACCT TAAAAAATTC 
TACAAAGTGG TTCCTGTCAA AGACATTAGG AAACTTTATG TGACTTTCCC CATCCCAGAT 
CTACAGCAGT ACTACAAATC GAAGCCAGGA CGTTATCTGG GACATCTGAT TGGTCACGAA 
GGACCTGGAA GTTTGTTTTC TGAGCTAAAA TCTAAAGGCT GGGTGGACAC AATCCTTGGA 
GGACACAAAG AGGGAGCCAG AGGATTCATG TTCTTCAACA TCAAGATGGA CTTGACTGAG 
GAGGGCCTCT TGAACATTGA GGACATAATC TTCCACATGT TCCAGTACAT CCAGAAACTC 
CGTTCTGAAG GGCCTCAAGA GTGGGTGTTT AACGAGTACA AGGATTTAAA AAAAGTTGCT 
TTCAGGTTTA AAGACAAGGA GCGCCCTCGT GACTACACTT CCAAAATAGC AGGTTTACTA 
CAGCACTACC CTCTTGAAGA AGTTCTATCA GCAGAGTACT TCTTGGAAGA CTTCAAGCCA 
GATCTGATCG AGATGGTGCT GGATAAGCTG AGACCAGAAC ACGTCAGAGT TATAGTTGTG 
TCAAAATCAT TTGAAGGACA AACAGACATA ACAGAAGAGT GGTACGGCAC GCAGTACAAA 
CAGGAGGCCA TCTCTGAGGA GACCATCAAG AAATGGGCAA ATGCAGACCT CAACGGCAAA 
TTCAAATTGC CAATGAAAAA TGAGTTCATC CCCACCAACT TCGAGATCTA CCCTCTTGAG 
AAAGACTCTC CGTCAGTTCC CACTTTAATC AAGGACACTG CTATGAGCAA GGTGTGGTTC 
AAGCAGGATG ACAAATTCTT CCTCCCCAAG GCTTGCCTGA ACTTTGAGTT CTTCAGCCCG 
TTTGCGTACG TGGACCCATT GCATTGTAAC ATGGCATATT TATACCTGGA GCTCCTCAAG 
GACTCCCTCA ACGAGTATGC ATATGCAGCC GAGCTAGCTG GCTTGAACTA TGAACTCCAA 
AATACCGCCT ATGGAATGCA TCTGTCTGTT AAAGGCTACA GTGACAAACA GAACATCCTG 
CTGAAGAAGA TCATCGAGAA GATGGCCACT TTTGAGATAG ACGAGAAGCG CTTTGACATC 
ATCAAGGAAG CTTACATGAG GTCTTTGAAT AACTTCAGAG CTGAGAGGCC TAACTATCAT 
GCTATGTACT ACCTCCATCT TCTAATGACC GAGGTTGTTT GGACCAAAGA TGAGCTCAGA 
GAGGCTCTAG ATGATGTAAC TCTCCCACGC CTTAACATCT TCATACCTCA GCTGTTGTCG 
CGGTTACATA TCAAAGTCCT TCTTCATGGC AACATCACCA AGGAGTCTGC TCTTGGCATG 
ATGCAAATGG TGGAGGACAC GCTCACTGAG CATGCTCACA CAAAGCCTCT CCTCCCGAGC 
CAGCTGATTC GCTACAGACA GGTGCAGGTT CCAGACGGTG GCTGGTATGT TTACCAGCAA 
AAGAACGCGG TGCACAATGA TTGTGGCATC GATATCTACT ACCAAATAGA CATGCAGAGT 
ACACACGACA ACATGCTGCT GGAGCTGTTC GCTCAGATCA TCTCTGAGCC CTGCTATAGT 
ACACTGAGAA CCAAAGAACA GCTGGGCTAC AGCGTGCATA GTGGGCCACG TCGGGCTAAC 
GGAGTGCAAG GTCTCTACTT CCTCATCCAG TCGGAGAAGG CGCCCCACTA CCTGGAGAGC 
CGAGTGGAAG CCTTCCTGTG TACTATGGAG AAAGCTGTGG AGGAGATGAG CGAGGAAGCC 
TTCCAGAAAC ACATCCAAGC CCTGGCCATC CGCCGCCTGG ACAAACCGAA GAAGCTGTCT 
GCTGAGTGTG CTAAATACTG GGGAGAGATC ATCTCTCAGC AGTACAATTT TGATAGAGAC 
AACATTGAAG TGGCGTATCT GAAGACTCTG ACAAAAGAAA ACATAATGGA GTTTTACAGA 
GAGCGACTGA CGGTCGAAGC CCCAAAGAGA CACAAAGTGT CGGTGCACGT GCTGTCCAGG 
GAGATGGACA CCTGTCCAGT AGTGGGAGAG TTCCCCGCTC AGAATGATGT CAACCTGGCT 
CCTGCTCCTT CCCTGCCACA GCCCACAGTG ATTCAGGACA TGATGGAGTT CAAGAGGAGT 
CTGCCTCTGT TCCCTCTGGT CAAACCTCAC ATCAACCTCA GGGCAGCCAA ACTGTGA