HIF1A cDNA ORF clone, Pteropus alecto(black flying fox)

Gene
Clones
gRNAs

Summary

Gene Symbol	HIF1A
Entrez Gene ID	102897284
Full Name	hypoxia inducible factor 1 alpha subunit
Gene Type	protein-coding
Organism	Pteropus alecto(black flying fox)

mRNA and Protein(s)

mRNA	Protein	Name
XM_006915104.3	XP_006915166.2	hypoxia-inducible factor 1-alpha
XM_006915104.2	XP_006915166.2	hypoxia-inducible factor 1-alpha

Pathways from BioSystems
Gene Ontology

The following HIF1A gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the HIF1A cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OPt18512	XM_006915104.3 Latest version!	Pteropus alecto hypoxia inducible factor 1 subunit alpha (HIF1A), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
View Old Accession Versions >>
OPt18512	XM_006915104.2	Pteropus alecto hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor) (HIF1A), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

HIF1A ( XM_006915104.3 ) cDNA ORF clone, Pteropus alecto(black flying fox) -> XP_006915166.2 Pteropus alecto hypoxia inducible factor 1 subunit alpha (HIF1A), mRNA.

Price & Availability↑

CloneID	OPt18512
Clone ID Related Accession (Same CDS sequence)	XM_006915104.3 , XM_006915104.2
Accession Version	XM_006915104.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2469bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Pteropus alecto(black flying fox)
Product	hypoxia-inducible factor 1-alpha
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_006438074.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_006915104.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pteropus alecto Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCAGGGA GGAAACATCT ACTTCAGACA AGGATAAGTT CTGAACGTCG AAAAGAAAAG 
TCTAGAGATG CAGCTAGATC TCGGCGAAGT AAAGAGTCTG AAGTTTTTTA TGAGCTTGCT 
CATCAGTTGC CACTTCCCCA TAATGTGAGT TCGCATCTTG ATAAGGCTTC TGTTATGAGG 
CTTACCATCA GCTATTTGCG TGTGAGGAAA CTTCTGGATG CTGGTGATTT GGATATTGAA 
GATGAAATGA AGGCACAGAT GAATTGCTTC TATTTGAAAG CCCTAGATGG TTTTGTTATG 
GTTCTCACAG ATGATGGTGA CATGATTTAC ATTTCTGATA ATGTGAACAA ATACATGGGA 
TTAACTCAGT TTGAACTAAC TGGACACAGT GTGTTTGATT TTACTCATCC GTGTGACCAT 
GAAGAAATGA GGGAAATGCT TACACACAGA AATGGCCTTG TGAAAAAGGG TAAAGAGCAA 
AATACACAGC GAAGCTTTTT CCTGAGAATG AAGTGTACCC TAACTAGCCG GGGGAGAACT 
ATGAACATAA AGTCTGCAAC ATGGAAGGTA CTTCATTGCA CAGGACATAT TCATGTATAC 
GATACCAATG GTAATCAGTC TCAGTGTGGG TATAAGAAAC CACCCATGAC ATGCTTGGTG 
CTGATTTGTG AACCCATTCC TCACCCATCA AATATTGAAA TTCCATTAGA TAGCAAGACT 
TTTCTCAGTC GTCACAGCCT GGATATGAAA TTTTCTTATT GTGATGAAAG AATTACTGAA 
TTGATGGGAT ATGAACCAGA AGAACTTTTG GGCCGTTCAA TTTATGAATA TTATCATGCT 
TTGGACTCTG ATCATCTGAC CAAAACTCAT CATGATATGT TTACTAAAGG ACAAGTCACC 
ACAGGACAGT ACAGGATGCT TGCCAAAAGA GGTGGATATG TCTGGGTTGA AACTCAAGCA 
ACTGTCATAT ACAACACTAA GAACTCTCAG CCGCAATGCA TTGTATGTGT AAATTACGTT 
GTGAGTGGTA TTATTCAGCA CGACTTGATT TTCTCCCTTC AACAAACAGA ATGTGTCCTC 
AAACCAGTTG AATCTTCAGA TATGAAAATG ACTCAGCTGT TCACCAAAGT TGAATCAGAA 
GATACAAGTA GCCTCTTTGA TAAGCTTAAG AAGGAACCTG ATGCTTTAAC TTTGCTGGCA 
CCAGCTGCTG GAGATACAAT CATATCTTTA GATTTCGGCA GCAATGACAC AGAAACTGAT 
GACCAACAAC TTGAGGAAGT TCCATTGTAT AATGATGTAA TGCTTCCCTC ATCCAGTGAA 
AAATTACAGA ATATAAATTT GTCGATGTCT CCATTACCTG CCTCTGAAAC TCCAAAGCCA 
CTTCGAAGTA GTGCTGACCC TGCACTCAAT CAGGAAGTTG CATTAAAATT AGAACCAAAT 
CCAGAGTCAC TGGAACTTTC CTTTACCATG CCTCAGATCC AAGATCAGCC AGCTAGTCCT 
TCTGATGGAA GCACTAGACA AAGTTCACCT GAGCCTACCA GTCCTAGTGA ATATTGTTTT 
GATGTGGATA GTGATATGGT CAATGAATTC AAATTGGAAT TGGTAGAGAA ACTTTTTGCT 
GAAGACACAG AAGCAAAGAA TCCATTTTCC ACTCAGGACA CTGATTTAGA CTTGGAGATG 
TTAGCTCCTT ATATCCCAAT GGATGATGAC TTCCAGTTAC GTTCCTTCGA TCAGTTGTCA 
CCATTGGAAA GCTGTTCTAC AAACCCTCAA AGTGCAAGCA CAATGACAGT ATTCCAGCCG 
ACTCAAATGC AAGAACCAGC TATTACCACC ACCACCGCCA CTGCCACCAC TGATGAATTA 
AAAACATTGA CAAAAGATGG TGTGGACGAC ATTAAAATAT TGATTGCATC TCCATCTCCT 
ACCCACATAC CTAAAGAAAC TACTAGTACC ACAACATCAC CGTATAGTGA TACTCGAAGT 
CGGACAGCCT CACCAAACAG AGCAGGAAAA GGAGTTATAG AACAAACAGA AAAATCTCAT 
CCAAGAAGCC CTAACGTGTT ATCTATCACT TTGAGTCAAA GAACTGCCGT TCCTGAGGAA 
GAACTAAATC CAAAGATACT AGCTTTACAG AATGCTCAAA GAAAGCGAAA AATAGAACAT 
GATGGTTCAC TCTTTCAAGC AGTAGGAATT GGAACATTAT TACAGCAGCC AGATGATCGT 
GCAGCTACTA CATCACTTTC TTGGAAACGT GTAAAAGGAT GCAAATCTAG TGAACAGAAT 
GGAATGGAGC AAAAGACAAT TATTTTAATA CCCTCTGATT TAGCATGTAG ACTGCTGGGG 
CAATCAATGG ATGAGAGTGG ATTACCACAG CTGACCAGTT ATGATTGTGA AGTTAATGCT 
CCTGTACAAG GCAGCAGAAA CCTACTTCAG GGTGAAGAAT TACTTAGAGC TTTGGATCAA 
GTTAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006915166.2
CDS	16..2484
Translation	MPGRKHLLQTRISSERRKEKSRDAARSRRSKESEVFYELAHQLPLPHNVSSHLDKASVMRLTISYLRVRKLLDAGDLDIEDEMKAQMNCFYLKALDGFVMVLTDDGDMIYISDNVNKYMGLTQFELTGHSVFDFTHPCDHEEMREMLTHRNGLVKKGKEQNTQRSFFLRMKCTLTSRGRTMNIKSATWKVLHCTGHIHVYDTNGNQSQCGYKKPPMTCLVLICEPIPHPSNIEIPLDSKTFLSRHSLDMKFSYCDERITELMGYEPEELLGRSIYEYYHALDSDHLTKTHHDMFTKGQVTTGQYRMLAKRGGYVWVETQATVIYNTKNSQPQCIVCVNYVVSGIIQHDLIFSLQQTECVLKPVESSDMKMTQLFTKVESEDTSSLFDKLKKEPDALTLLAPAAGDTIISLDFGSNDTETDDQQLEEVPLYNDVMLPSSSEKLQNINLSMSPLPASETPKPLRSSADPALNQEVALKLEPNPESLELSFTMPQIQDQPASPSDGSTRQSSPEPTSPSEYCFDVDSDMVNEFKLELVEKLFAEDTEAKNPFSTQDTDLDLEMLAPYIPMDDDFQLRSFDQLSPLESCSTNPQSASTMTVFQPTQMQEPAITTTTATATTDELKTLTKDGVDDIKILIASPSPTHIPKETTSTTTSPYSDTRSRTASPNRAGKGVIEQTEKSHPRSPNVLSITLSQRTAVPEEELNPKILALQNAQRKRKIEHDGSLFQAVGIGTLLQQPDDRAATTSLSWKRVKGCKSSEQNGMEQKTIILIPSDLACRLLGQSMDESGLPQLTSYDCEVNAPVQGSRNLLQGEELLRALDQVN

Target ORF information:

RefSeq Version	XM_006915104.3
Organism	Pteropus alecto(black flying fox)
Definition	Pteropus alecto hypoxia inducible factor 1 subunit alpha (HIF1A), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006915104.3

ORF Insert Sequence:

ATGCCAGGGA GGAAACATCT ACTTCAGACA AGGATAAGTT CTGAACGTCG AAAAGAAAAG 
TCTAGAGATG CAGCTAGATC TCGGCGAAGT AAAGAGTCTG AAGTTTTTTA TGAGCTTGCT 
CATCAGTTGC CACTTCCCCA TAATGTGAGT TCGCATCTTG ATAAGGCTTC TGTTATGAGG 
CTTACCATCA GCTATTTGCG TGTGAGGAAA CTTCTGGATG CTGGTGATTT GGATATTGAA 
GATGAAATGA AGGCACAGAT GAATTGCTTC TATTTGAAAG CCCTAGATGG TTTTGTTATG 
GTTCTCACAG ATGATGGTGA CATGATTTAC ATTTCTGATA ATGTGAACAA ATACATGGGA 
TTAACTCAGT TTGAACTAAC TGGACACAGT GTGTTTGATT TTACTCATCC GTGTGACCAT 
GAAGAAATGA GGGAAATGCT TACACACAGA AATGGCCTTG TGAAAAAGGG TAAAGAGCAA 
AATACACAGC GAAGCTTTTT CCTGAGAATG AAGTGTACCC TAACTAGCCG GGGGAGAACT 
ATGAACATAA AGTCTGCAAC ATGGAAGGTA CTTCATTGCA CAGGACATAT TCATGTATAC 
GATACCAATG GTAATCAGTC TCAGTGTGGG TATAAGAAAC CACCCATGAC ATGCTTGGTG 
CTGATTTGTG AACCCATTCC TCACCCATCA AATATTGAAA TTCCATTAGA TAGCAAGACT 
TTTCTCAGTC GTCACAGCCT GGATATGAAA TTTTCTTATT GTGATGAAAG AATTACTGAA 
TTGATGGGAT ATGAACCAGA AGAACTTTTG GGCCGTTCAA TTTATGAATA TTATCATGCT 
TTGGACTCTG ATCATCTGAC CAAAACTCAT CATGATATGT TTACTAAAGG ACAAGTCACC 
ACAGGACAGT ACAGGATGCT TGCCAAAAGA GGTGGATATG TCTGGGTTGA AACTCAAGCA 
ACTGTCATAT ACAACACTAA GAACTCTCAG CCGCAATGCA TTGTATGTGT AAATTACGTT 
GTGAGTGGTA TTATTCAGCA CGACTTGATT TTCTCCCTTC AACAAACAGA ATGTGTCCTC 
AAACCAGTTG AATCTTCAGA TATGAAAATG ACTCAGCTGT TCACCAAAGT TGAATCAGAA 
GATACAAGTA GCCTCTTTGA TAAGCTTAAG AAGGAACCTG ATGCTTTAAC TTTGCTGGCA 
CCAGCTGCTG GAGATACAAT CATATCTTTA GATTTCGGCA GCAATGACAC AGAAACTGAT 
GACCAACAAC TTGAGGAAGT TCCATTGTAT AATGATGTAA TGCTTCCCTC ATCCAGTGAA 
AAATTACAGA ATATAAATTT GTCGATGTCT CCATTACCTG CCTCTGAAAC TCCAAAGCCA 
CTTCGAAGTA GTGCTGACCC TGCACTCAAT CAGGAAGTTG CATTAAAATT AGAACCAAAT 
CCAGAGTCAC TGGAACTTTC CTTTACCATG CCTCAGATCC AAGATCAGCC AGCTAGTCCT 
TCTGATGGAA GCACTAGACA AAGTTCACCT GAGCCTACCA GTCCTAGTGA ATATTGTTTT 
GATGTGGATA GTGATATGGT CAATGAATTC AAATTGGAAT TGGTAGAGAA ACTTTTTGCT 
GAAGACACAG AAGCAAAGAA TCCATTTTCC ACTCAGGACA CTGATTTAGA CTTGGAGATG 
TTAGCTCCTT ATATCCCAAT GGATGATGAC TTCCAGTTAC GTTCCTTCGA TCAGTTGTCA 
CCATTGGAAA GCTGTTCTAC AAACCCTCAA AGTGCAAGCA CAATGACAGT ATTCCAGCCG 
ACTCAAATGC AAGAACCAGC TATTACCACC ACCACCGCCA CTGCCACCAC TGATGAATTA 
AAAACATTGA CAAAAGATGG TGTGGACGAC ATTAAAATAT TGATTGCATC TCCATCTCCT 
ACCCACATAC CTAAAGAAAC TACTAGTACC ACAACATCAC CGTATAGTGA TACTCGAAGT 
CGGACAGCCT CACCAAACAG AGCAGGAAAA GGAGTTATAG AACAAACAGA AAAATCTCAT 
CCAAGAAGCC CTAACGTGTT ATCTATCACT TTGAGTCAAA GAACTGCCGT TCCTGAGGAA 
GAACTAAATC CAAAGATACT AGCTTTACAG AATGCTCAAA GAAAGCGAAA AATAGAACAT 
GATGGTTCAC TCTTTCAAGC AGTAGGAATT GGAACATTAT TACAGCAGCC AGATGATCGT 
GCAGCTACTA CATCACTTTC TTGGAAACGT GTAAAAGGAT GCAAATCTAG TGAACAGAAT 
GGAATGGAGC AAAAGACAAT TATTTTAATA CCCTCTGATT TAGCATGTAG ACTGCTGGGG 
CAATCAATGG ATGAGAGTGG ATTACCACAG CTGACCAGTT ATGATTGTGA AGTTAATGCT 
CCTGTACAAG GCAGCAGAAA CCTACTTCAG GGTGAAGAAT TACTTAGAGC TTTGGATCAA 
GTTAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HIF1A ( XM_006915104.2 ) cDNA ORF clone, Pteropus alecto(black flying fox) -> XP_006915166.2 Pteropus alecto hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor) (HIF1A), mRNA.

Price & Availability↑

CloneID	OPt18512
Clone ID Related Accession (Same CDS sequence)	XM_006915104.3 , XM_006915104.2
Accession Version	XM_006915104.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2469bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-02-09
Organism	Pteropus alecto(black flying fox)
Product	hypoxia-inducible factor 1-alpha
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_006438074.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process On Feb 10, 2016 this sequence version replaced XM_006915104.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pteropus alecto Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCAGGGA GGAAACATCT ACTTCAGACA AGGATAAGTT CTGAACGTCG AAAAGAAAAG 
TCTAGAGATG CAGCTAGATC TCGGCGAAGT AAAGAGTCTG AAGTTTTTTA TGAGCTTGCT 
CATCAGTTGC CACTTCCCCA TAATGTGAGT TCGCATCTTG ATAAGGCTTC TGTTATGAGG 
CTTACCATCA GCTATTTGCG TGTGAGGAAA CTTCTGGATG CTGGTGATTT GGATATTGAA 
GATGAAATGA AGGCACAGAT GAATTGCTTC TATTTGAAAG CCCTAGATGG TTTTGTTATG 
GTTCTCACAG ATGATGGTGA CATGATTTAC ATTTCTGATA ATGTGAACAA ATACATGGGA 
TTAACTCAGT TTGAACTAAC TGGACACAGT GTGTTTGATT TTACTCATCC GTGTGACCAT 
GAAGAAATGA GGGAAATGCT TACACACAGA AATGGCCTTG TGAAAAAGGG TAAAGAGCAA 
AATACACAGC GAAGCTTTTT CCTGAGAATG AAGTGTACCC TAACTAGCCG GGGGAGAACT 
ATGAACATAA AGTCTGCAAC ATGGAAGGTA CTTCATTGCA CAGGACATAT TCATGTATAC 
GATACCAATG GTAATCAGTC TCAGTGTGGG TATAAGAAAC CACCCATGAC ATGCTTGGTG 
CTGATTTGTG AACCCATTCC TCACCCATCA AATATTGAAA TTCCATTAGA TAGCAAGACT 
TTTCTCAGTC GTCACAGCCT GGATATGAAA TTTTCTTATT GTGATGAAAG AATTACTGAA 
TTGATGGGAT ATGAACCAGA AGAACTTTTG GGCCGTTCAA TTTATGAATA TTATCATGCT 
TTGGACTCTG ATCATCTGAC CAAAACTCAT CATGATATGT TTACTAAAGG ACAAGTCACC 
ACAGGACAGT ACAGGATGCT TGCCAAAAGA GGTGGATATG TCTGGGTTGA AACTCAAGCA 
ACTGTCATAT ACAACACTAA GAACTCTCAG CCGCAATGCA TTGTATGTGT AAATTACGTT 
GTGAGTGGTA TTATTCAGCA CGACTTGATT TTCTCCCTTC AACAAACAGA ATGTGTCCTC 
AAACCAGTTG AATCTTCAGA TATGAAAATG ACTCAGCTGT TCACCAAAGT TGAATCAGAA 
GATACAAGTA GCCTCTTTGA TAAGCTTAAG AAGGAACCTG ATGCTTTAAC TTTGCTGGCA 
CCAGCTGCTG GAGATACAAT CATATCTTTA GATTTCGGCA GCAATGACAC AGAAACTGAT 
GACCAACAAC TTGAGGAAGT TCCATTGTAT AATGATGTAA TGCTTCCCTC ATCCAGTGAA 
AAATTACAGA ATATAAATTT GTCGATGTCT CCATTACCTG CCTCTGAAAC TCCAAAGCCA 
CTTCGAAGTA GTGCTGACCC TGCACTCAAT CAGGAAGTTG CATTAAAATT AGAACCAAAT 
CCAGAGTCAC TGGAACTTTC CTTTACCATG CCTCAGATCC AAGATCAGCC AGCTAGTCCT 
TCTGATGGAA GCACTAGACA AAGTTCACCT GAGCCTACCA GTCCTAGTGA ATATTGTTTT 
GATGTGGATA GTGATATGGT CAATGAATTC AAATTGGAAT TGGTAGAGAA ACTTTTTGCT 
GAAGACACAG AAGCAAAGAA TCCATTTTCC ACTCAGGACA CTGATTTAGA CTTGGAGATG 
TTAGCTCCTT ATATCCCAAT GGATGATGAC TTCCAGTTAC GTTCCTTCGA TCAGTTGTCA 
CCATTGGAAA GCTGTTCTAC AAACCCTCAA AGTGCAAGCA CAATGACAGT ATTCCAGCCG 
ACTCAAATGC AAGAACCAGC TATTACCACC ACCACCGCCA CTGCCACCAC TGATGAATTA 
AAAACATTGA CAAAAGATGG TGTGGACGAC ATTAAAATAT TGATTGCATC TCCATCTCCT 
ACCCACATAC CTAAAGAAAC TACTAGTACC ACAACATCAC CGTATAGTGA TACTCGAAGT 
CGGACAGCCT CACCAAACAG AGCAGGAAAA GGAGTTATAG AACAAACAGA AAAATCTCAT 
CCAAGAAGCC CTAACGTGTT ATCTATCACT TTGAGTCAAA GAACTGCCGT TCCTGAGGAA 
GAACTAAATC CAAAGATACT AGCTTTACAG AATGCTCAAA GAAAGCGAAA AATAGAACAT 
GATGGTTCAC TCTTTCAAGC AGTAGGAATT GGAACATTAT TACAGCAGCC AGATGATCGT 
GCAGCTACTA CATCACTTTC TTGGAAACGT GTAAAAGGAT GCAAATCTAG TGAACAGAAT 
GGAATGGAGC AAAAGACAAT TATTTTAATA CCCTCTGATT TAGCATGTAG ACTGCTGGGG 
CAATCAATGG ATGAGAGTGG ATTACCACAG CTGACCAGTT ATGATTGTGA AGTTAATGCT 
CCTGTACAAG GCAGCAGAAA CCTACTTCAG GGTGAAGAAT TACTTAGAGC TTTGGATCAA 
GTTAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006915166.2
CDS	16..2484
Translation	MPGRKHLLQTRISSERRKEKSRDAARSRRSKESEVFYELAHQLPLPHNVSSHLDKASVMRLTISYLRVRKLLDAGDLDIEDEMKAQMNCFYLKALDGFVMVLTDDGDMIYISDNVNKYMGLTQFELTGHSVFDFTHPCDHEEMREMLTHRNGLVKKGKEQNTQRSFFLRMKCTLTSRGRTMNIKSATWKVLHCTGHIHVYDTNGNQSQCGYKKPPMTCLVLICEPIPHPSNIEIPLDSKTFLSRHSLDMKFSYCDERITELMGYEPEELLGRSIYEYYHALDSDHLTKTHHDMFTKGQVTTGQYRMLAKRGGYVWVETQATVIYNTKNSQPQCIVCVNYVVSGIIQHDLIFSLQQTECVLKPVESSDMKMTQLFTKVESEDTSSLFDKLKKEPDALTLLAPAAGDTIISLDFGSNDTETDDQQLEEVPLYNDVMLPSSSEKLQNINLSMSPLPASETPKPLRSSADPALNQEVALKLEPNPESLELSFTMPQIQDQPASPSDGSTRQSSPEPTSPSEYCFDVDSDMVNEFKLELVEKLFAEDTEAKNPFSTQDTDLDLEMLAPYIPMDDDFQLRSFDQLSPLESCSTNPQSASTMTVFQPTQMQEPAITTTTATATTDELKTLTKDGVDDIKILIASPSPTHIPKETTSTTTSPYSDTRSRTASPNRAGKGVIEQTEKSHPRSPNVLSITLSQRTAVPEEELNPKILALQNAQRKRKIEHDGSLFQAVGIGTLLQQPDDRAATTSLSWKRVKGCKSSEQNGMEQKTIILIPSDLACRLLGQSMDESGLPQLTSYDCEVNAPVQGSRNLLQGEELLRALDQVN

Target ORF information:

RefSeq Version	XM_006915104.2
Organism	Pteropus alecto(black flying fox)
Definition	Pteropus alecto hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor) (HIF1A), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006915104.2

ORF Insert Sequence:

ATGCCAGGGA GGAAACATCT ACTTCAGACA AGGATAAGTT CTGAACGTCG AAAAGAAAAG 
TCTAGAGATG CAGCTAGATC TCGGCGAAGT AAAGAGTCTG AAGTTTTTTA TGAGCTTGCT 
CATCAGTTGC CACTTCCCCA TAATGTGAGT TCGCATCTTG ATAAGGCTTC TGTTATGAGG 
CTTACCATCA GCTATTTGCG TGTGAGGAAA CTTCTGGATG CTGGTGATTT GGATATTGAA 
GATGAAATGA AGGCACAGAT GAATTGCTTC TATTTGAAAG CCCTAGATGG TTTTGTTATG 
GTTCTCACAG ATGATGGTGA CATGATTTAC ATTTCTGATA ATGTGAACAA ATACATGGGA 
TTAACTCAGT TTGAACTAAC TGGACACAGT GTGTTTGATT TTACTCATCC GTGTGACCAT 
GAAGAAATGA GGGAAATGCT TACACACAGA AATGGCCTTG TGAAAAAGGG TAAAGAGCAA 
AATACACAGC GAAGCTTTTT CCTGAGAATG AAGTGTACCC TAACTAGCCG GGGGAGAACT 
ATGAACATAA AGTCTGCAAC ATGGAAGGTA CTTCATTGCA CAGGACATAT TCATGTATAC 
GATACCAATG GTAATCAGTC TCAGTGTGGG TATAAGAAAC CACCCATGAC ATGCTTGGTG 
CTGATTTGTG AACCCATTCC TCACCCATCA AATATTGAAA TTCCATTAGA TAGCAAGACT 
TTTCTCAGTC GTCACAGCCT GGATATGAAA TTTTCTTATT GTGATGAAAG AATTACTGAA 
TTGATGGGAT ATGAACCAGA AGAACTTTTG GGCCGTTCAA TTTATGAATA TTATCATGCT 
TTGGACTCTG ATCATCTGAC CAAAACTCAT CATGATATGT TTACTAAAGG ACAAGTCACC 
ACAGGACAGT ACAGGATGCT TGCCAAAAGA GGTGGATATG TCTGGGTTGA AACTCAAGCA 
ACTGTCATAT ACAACACTAA GAACTCTCAG CCGCAATGCA TTGTATGTGT AAATTACGTT 
GTGAGTGGTA TTATTCAGCA CGACTTGATT TTCTCCCTTC AACAAACAGA ATGTGTCCTC 
AAACCAGTTG AATCTTCAGA TATGAAAATG ACTCAGCTGT TCACCAAAGT TGAATCAGAA 
GATACAAGTA GCCTCTTTGA TAAGCTTAAG AAGGAACCTG ATGCTTTAAC TTTGCTGGCA 
CCAGCTGCTG GAGATACAAT CATATCTTTA GATTTCGGCA GCAATGACAC AGAAACTGAT 
GACCAACAAC TTGAGGAAGT TCCATTGTAT AATGATGTAA TGCTTCCCTC ATCCAGTGAA 
AAATTACAGA ATATAAATTT GTCGATGTCT CCATTACCTG CCTCTGAAAC TCCAAAGCCA 
CTTCGAAGTA GTGCTGACCC TGCACTCAAT CAGGAAGTTG CATTAAAATT AGAACCAAAT 
CCAGAGTCAC TGGAACTTTC CTTTACCATG CCTCAGATCC AAGATCAGCC AGCTAGTCCT 
TCTGATGGAA GCACTAGACA AAGTTCACCT GAGCCTACCA GTCCTAGTGA ATATTGTTTT 
GATGTGGATA GTGATATGGT CAATGAATTC AAATTGGAAT TGGTAGAGAA ACTTTTTGCT 
GAAGACACAG AAGCAAAGAA TCCATTTTCC ACTCAGGACA CTGATTTAGA CTTGGAGATG 
TTAGCTCCTT ATATCCCAAT GGATGATGAC TTCCAGTTAC GTTCCTTCGA TCAGTTGTCA 
CCATTGGAAA GCTGTTCTAC AAACCCTCAA AGTGCAAGCA CAATGACAGT ATTCCAGCCG 
ACTCAAATGC AAGAACCAGC TATTACCACC ACCACCGCCA CTGCCACCAC TGATGAATTA 
AAAACATTGA CAAAAGATGG TGTGGACGAC ATTAAAATAT TGATTGCATC TCCATCTCCT 
ACCCACATAC CTAAAGAAAC TACTAGTACC ACAACATCAC CGTATAGTGA TACTCGAAGT 
CGGACAGCCT CACCAAACAG AGCAGGAAAA GGAGTTATAG AACAAACAGA AAAATCTCAT 
CCAAGAAGCC CTAACGTGTT ATCTATCACT TTGAGTCAAA GAACTGCCGT TCCTGAGGAA 
GAACTAAATC CAAAGATACT AGCTTTACAG AATGCTCAAA GAAAGCGAAA AATAGAACAT 
GATGGTTCAC TCTTTCAAGC AGTAGGAATT GGAACATTAT TACAGCAGCC AGATGATCGT 
GCAGCTACTA CATCACTTTC TTGGAAACGT GTAAAAGGAT GCAAATCTAG TGAACAGAAT 
GGAATGGAGC AAAAGACAAT TATTTTAATA CCCTCTGATT TAGCATGTAG ACTGCTGGGG 
CAATCAATGG ATGAGAGTGG ATTACCACAG CTGACCAGTT ATGATTGTGA AGTTAATGCT 
CCTGTACAAG GCAGCAGAAA CCTACTTCAG GGTGAAGAAT TACTTAGAGC TTTGGATCAA 
GTTAACTGA