TLR7 cDNA ORF clone, Pseudopodoces humilis(Tibetan ground-tit)

Gene
Clones
gRNAs

Summary

Gene Symbol	TLR7
Entrez Gene ID	102112976
Full Name	toll like receptor 7
Gene Type	protein-coding
Organism	Pseudopodoces humilis(Tibetan ground-tit)

mRNA and Protein(s)

mRNA	Protein	Name
XM_014252883.1	XP_014108358.1	toll-like receptor 7 isoform X2
XM_014252874.1	XP_014108349.1	toll-like receptor 7 isoform X1

Pathways from BioSystems
Gene Ontology

The following TLR7 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TLR7 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OPs05844	XM_014252874.1 Latest version!	Pseudopodoces humilis toll-like receptor 7 (TLR7), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OPs05845	XM_014252883.1 Latest version!	Pseudopodoces humilis toll-like receptor 7 (TLR7), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

TLR7 ( XM_014252874.1 ) cDNA ORF clone, Pseudopodoces humilis(Tibetan ground-tit) -> XP_014108349.1 Pseudopodoces humilis toll-like receptor 7 (TLR7), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OPs05844
Clone ID Related Accession (Same CDS sequence)	XM_014252874.1
Accession Version	XM_014252874.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3273bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-20
Organism	Pseudopodoces humilis(Tibetan ground-tit)
Product	toll-like receptor 7 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005087554.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pseudopodoces humilis Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTAGAAA GGCTGCTAGA CTTTGATGTG CATCAGTTGT CAGTATTTGC TTCAGTGACT 
GCAGATTCCA TGTCCCCAGC TGGTGACAGC CAGCACGTCA TTGTTTGCTG CGGTGTGTGT 
CAACAAATAA AGGTACCTTG TGCAAAGATG TCAAATGCAT TGCCATTTAT CTTGCTCTTC 
ATATTCCCAA TGCTGCTGTC TGGGGCTTGG TTTCCCAAAA CTTTACCCTG TGATGTTAAA 
TCTTCAGAAG GTGCTGTGAC AGTGGACTGC ACTGACCGGC GCCTTACAGA AGTCCCCAGA 
GGGATCCCTG GAAATGCTAC CAACCTTACC CTGAGTATTA ATCATATTCC CCATATCTAC 
CCAACATCCT TCAATCATCT TGAAAACCTC GAGGAGATTG ACTTCAGATG CAACTGTGTG 
CCTGTCAAGC TGGGGCCCAA AAATCATGTG TGCACCAGCC CCCTGAAAAT TGAGAATGGT 
AGTTTTGCTG CCTTGACAAG ATTGAAGGCA TTGTACCTGG ATGCAAACCA GCTGGCAGAA 
GTACCCCGAG GTCTTCCTGC TGCTTTAAGC CTGCTGAGTC TGGAAGCAAA CAGTATCTTT 
TCTATCCAAA AAGCCAGCTT CTCGGAGCTA GGGAACATAG AGGTGCTGTA TCTTGGACAG 
AACTGTTACT ACCGCAATCC ATGCAATGTT TCATTTGAAA TTGAGAAAAC AGCCTTTCTG 
GGGCTGAAAA AGTTAACAAT ACTATCCCTG AAGTCCAACA ACTTAACACA AATTCCTCCC 
AATTTGTCAT CTACTTTAAA GGAATTGTAT ATTTACAACA ACATGATTCA AGAGATTCAA 
GAGCAGGATT TAAGTGGTCT TCCCAACCTA GAAATTCTCG ACCTAAGTGG CAATTGCCCA 
CGTTGCTATA ATGCCCCATA TCCCTGCGTT CCCTGTCCCA AGAGCTCCAT TCAGATACAT 
TCAAATGCTT TTGATTCTTT GGAAAAATTA AGAATTTTGC GGCTTCACAG TAACTCTCTA 
CAGAGCATAC CCAGCAGCTG GTTTAAAAAC TTCAGGAATC TCAAAGAACT TGACCTCTCC 
CGAAATTTCC TCATGAGGGA GATTGGAGAT GCTCAGTTTT TGACATTTAT CCCCAGTCTT 
GTGCAGCTTG ATCTGTCCTT TAATTTTGAA CTGAAGGTGT ATTCTCCCTT CTTGGATCTT 
TCTGAGACAT TTTCTACCCT CTCTAACCTG GAAATCCTGA GACTCAAGGG TTATGTCTTT 
AAAGAGCTGA GGGCACAAGA TCTATATCCA CTGCTCAGTC TTAGGAATCT AACTATGTTG 
GATCTCGGGA CTAATTTTAT TAAAATTGCA AACCTAACTG TGTTTGATGA ATTCCCAGCT 
CTTAAGTTCA TTGATCTCTC AGTGAATAAA ATTTCTCCTT CTTCAGGGGA AGGCAACCTC 
TATGGATTTT GCTCTAATCC TGCCATTTCA GCCGAGCAAT ACAACAGACA AGTACAACAA 
GAGATGCATT ATTTTAGATA TGATGTGTAT GAGCGAAGTT GCCGTTCCAA AGACAAAGAG 
GATTCTTCTT ACCAGTCTTT AGTTAAGGAA GATTGCCTTA ACTATGGAAA AACATTGGAT 
TTAAGCAGAA ACAATGTATT TTTTGTTAAC CCCTCTGACT TTCAGGGGCT TAGCTCCCTG 
AAATGTCTTA ACTTGTCAGG TAATGCAATA AGTCAAACTT TGAATGGAAG TGAATTCTTT 
TACTTGTCTG GATTGAAATA TCTGGATTTT TCTAACAACA GGGTTGATTT GTTATACCAA 
ACTGCTTTCA AAGAACTAAA ATATTTAGAA ATTCTAGATC TGAGCAATAA CAACTATTAC 
TTTCTGGCAG AAGGTGTTAC TCACGTGCTA AGTTTTATGA AAAACCTACC CCACTTGAGG 
AAGCTGATGA TGAATGACAA TGATATTTCC ACCACTATTG ATACAGGAAT GGAAAGTCAA 
TCTCTTCGAA TTTTAGAATT CAGAGGAAAT CACTTAGATG TTCTCTGGAA GGATGGCAAT 
GATAGATACT TGTCATTCTT CAAGAATCTG AGCAGCCTGG AAGAACTGGA TATTTCCTTC 
AACTCACTCA GCTTTTTGCC TCATAGTGTT TTTGAAAAAA TGCCTCCCAG TCTCAAGGTG 
CTCAACCTAA CAAATAATCA GCTGAAGAGT TTCATTTGGG GAAACCTCCC CTCTCTGAAG 
AACCTGGTAA CTCTGGACCT GAGCAATAAC CTTCTGACTA ATGTTCCCCG AGAACTGTCC 
AATTGCACTT CATCGCTGCA GGTACTGATC CTGCGAAACA ATCGCATTCA CGTGCTAACC 
AAACATTTTC TCAGGGGTGC TTTTGAACTG AGATACTTGG ACCTCAGCTC AAATAAGATT 
GAAATAATTA AGAGATCTAG CTTCCCTGAA AATGTCATTA ACAACCTGAA GATGCTGCTT 
TTGCACGGCA ATCCCTTTAA GTGTAACTGT GAGGCTGTGT GGTTTGTCTG GTGGATCAAC 
CGGACGCAGG TGACCATTCC TCTTCTGGCC ACGGACGTCA CCTGTGCTGG CCCAGGGGCA 
CATAAGGGAA GGAGCGTGGT TTTCCTGGAC CTGTACACCT GTGAGCTGGA CACGTCCTAT 
TTGATCCTGT ACGCTCTGTC AGCTTCAGCC ATCCTCGGCT TGGTGGTGTT CACTGTGATG 
AGCCATCTCT ACTTCTGGGA TGTGTGGTAC AGCTACCATT ACTGCGCTGC CAAGCTGAAG 
GGCTATCAGC GCTTGTCGTC ACCAGCCGCT TGCTACGATG CCTTTATTGC CTATGACAGT 
GAAGATCCTG CTGTGAACGA GTGGGTACTG CAAGAACTGG TTGAAAGGCT GGAAAACCAA 
AAAGCCAGGC AGTTCAATTT ATGCCTGGAA GGAAGGGACT GGCTCCCAGG ACAGCCGGTC 
TTTGACAACC TTTCCCAGAG CATTCAGCTG AGCAAAAAGA CCATATTTGT GCTGACCAAC 
AGGTATATTA AAAGTGGCCG CTTCAAGACA ACATTTTATA TGGCTCATCA GCGCCTTCTA 
GATGAAAAAA TGGATGTCAT TATCTTGATA TTTCTTGAGA AGGTTTTGCA GCAGTCCCGC 
TATGTCCGTC TGAGGAAGAG GCTGTGCAGA AGTTCAGTCC TGGAATGGCC AACTAATCCT 
CAGTCTCAGC CCTACTTCTG GCAGTGCCTG AAAAATGCCA TAGCTACGAG CAATTCTCTG 
GCATACAACA AGCTTCTTCA AGAAACTGTT TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014108349.1
CDS	48..3320
Translation	MVERLLDFDVHQLSVFASVTADSMSPAGDSQHVIVCCGVCQQIKVPCAKMSNALPFILLFIFPMLLSGAWFPKTLPCDVKSSEGAVTVDCTDRRLTEVPRGIPGNATNLTLSINHIPHIYPTSFNHLENLEEIDFRCNCVPVKLGPKNHVCTSPLKIENGSFAALTRLKALYLDANQLAEVPRGLPAALSLLSLEANSIFSIQKASFSELGNIEVLYLGQNCYYRNPCNVSFEIEKTAFLGLKKLTILSLKSNNLTQIPPNLSSTLKELYIYNNMIQEIQEQDLSGLPNLEILDLSGNCPRCYNAPYPCVPCPKSSIQIHSNAFDSLEKLRILRLHSNSLQSIPSSWFKNFRNLKELDLSRNFLMREIGDAQFLTFIPSLVQLDLSFNFELKVYSPFLDLSETFSTLSNLEILRLKGYVFKELRAQDLYPLLSLRNLTMLDLGTNFIKIANLTVFDEFPALKFIDLSVNKISPSSGEGNLYGFCSNPAISAEQYNRQVQQEMHYFRYDVYERSCRSKDKEDSSYQSLVKEDCLNYGKTLDLSRNNVFFVNPSDFQGLSSLKCLNLSGNAISQTLNGSEFFYLSGLKYLDFSNNRVDLLYQTAFKELKYLEILDLSNNNYYFLAEGVTHVLSFMKNLPHLRKLMMNDNDISTTIDTGMESQSLRILEFRGNHLDVLWKDGNDRYLSFFKNLSSLEELDISFNSLSFLPHSVFEKMPPSLKVLNLTNNQLKSFIWGNLPSLKNLVTLDLSNNLLTNVPRELSNCTSSLQVLILRNNRIHVLTKHFLRGAFELRYLDLSSNKIEIIKRSSFPENVINNLKMLLLHGNPFKCNCEAVWFVWWINRTQVTIPLLATDVTCAGPGAHKGRSVVFLDLYTCELDTSYLILYALSASAILGLVVFTVMSHLYFWDVWYSYHYCAAKLKGYQRLSSPAACYDAFIAYDSEDPAVNEWVLQELVERLENQKARQFNLCLEGRDWLPGQPVFDNLSQSIQLSKKTIFVLTNRYIKSGRFKTTFYMAHQRLLDEKMDVIILIFLEKVLQQSRYVRLRKRLCRSSVLEWPTNPQSQPYFWQCLKNAIATSNSLAYNKLLQETV

Target ORF information:

RefSeq Version	XM_014252874.1
Organism	Pseudopodoces humilis(Tibetan ground-tit)
Definition	Pseudopodoces humilis toll-like receptor 7 (TLR7), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014252874.1

ORF Insert Sequence:

ATGGTAGAAA GGCTGCTAGA CTTTGATGTG CATCAGTTGT CAGTATTTGC TTCAGTGACT 
GCAGATTCCA TGTCCCCAGC TGGTGACAGC CAGCACGTCA TTGTTTGCTG CGGTGTGTGT 
CAACAAATAA AGGTACCTTG TGCAAAGATG TCAAATGCAT TGCCATTTAT CTTGCTCTTC 
ATATTCCCAA TGCTGCTGTC TGGGGCTTGG TTTCCCAAAA CTTTACCCTG TGATGTTAAA 
TCTTCAGAAG GTGCTGTGAC AGTGGACTGC ACTGACCGGC GCCTTACAGA AGTCCCCAGA 
GGGATCCCTG GAAATGCTAC CAACCTTACC CTGAGTATTA ATCATATTCC CCATATCTAC 
CCAACATCCT TCAATCATCT TGAAAACCTC GAGGAGATTG ACTTCAGATG CAACTGTGTG 
CCTGTCAAGC TGGGGCCCAA AAATCATGTG TGCACCAGCC CCCTGAAAAT TGAGAATGGT 
AGTTTTGCTG CCTTGACAAG ATTGAAGGCA TTGTACCTGG ATGCAAACCA GCTGGCAGAA 
GTACCCCGAG GTCTTCCTGC TGCTTTAAGC CTGCTGAGTC TGGAAGCAAA CAGTATCTTT 
TCTATCCAAA AAGCCAGCTT CTCGGAGCTA GGGAACATAG AGGTGCTGTA TCTTGGACAG 
AACTGTTACT ACCGCAATCC ATGCAATGTT TCATTTGAAA TTGAGAAAAC AGCCTTTCTG 
GGGCTGAAAA AGTTAACAAT ACTATCCCTG AAGTCCAACA ACTTAACACA AATTCCTCCC 
AATTTGTCAT CTACTTTAAA GGAATTGTAT ATTTACAACA ACATGATTCA AGAGATTCAA 
GAGCAGGATT TAAGTGGTCT TCCCAACCTA GAAATTCTCG ACCTAAGTGG CAATTGCCCA 
CGTTGCTATA ATGCCCCATA TCCCTGCGTT CCCTGTCCCA AGAGCTCCAT TCAGATACAT 
TCAAATGCTT TTGATTCTTT GGAAAAATTA AGAATTTTGC GGCTTCACAG TAACTCTCTA 
CAGAGCATAC CCAGCAGCTG GTTTAAAAAC TTCAGGAATC TCAAAGAACT TGACCTCTCC 
CGAAATTTCC TCATGAGGGA GATTGGAGAT GCTCAGTTTT TGACATTTAT CCCCAGTCTT 
GTGCAGCTTG ATCTGTCCTT TAATTTTGAA CTGAAGGTGT ATTCTCCCTT CTTGGATCTT 
TCTGAGACAT TTTCTACCCT CTCTAACCTG GAAATCCTGA GACTCAAGGG TTATGTCTTT 
AAAGAGCTGA GGGCACAAGA TCTATATCCA CTGCTCAGTC TTAGGAATCT AACTATGTTG 
GATCTCGGGA CTAATTTTAT TAAAATTGCA AACCTAACTG TGTTTGATGA ATTCCCAGCT 
CTTAAGTTCA TTGATCTCTC AGTGAATAAA ATTTCTCCTT CTTCAGGGGA AGGCAACCTC 
TATGGATTTT GCTCTAATCC TGCCATTTCA GCCGAGCAAT ACAACAGACA AGTACAACAA 
GAGATGCATT ATTTTAGATA TGATGTGTAT GAGCGAAGTT GCCGTTCCAA AGACAAAGAG 
GATTCTTCTT ACCAGTCTTT AGTTAAGGAA GATTGCCTTA ACTATGGAAA AACATTGGAT 
TTAAGCAGAA ACAATGTATT TTTTGTTAAC CCCTCTGACT TTCAGGGGCT TAGCTCCCTG 
AAATGTCTTA ACTTGTCAGG TAATGCAATA AGTCAAACTT TGAATGGAAG TGAATTCTTT 
TACTTGTCTG GATTGAAATA TCTGGATTTT TCTAACAACA GGGTTGATTT GTTATACCAA 
ACTGCTTTCA AAGAACTAAA ATATTTAGAA ATTCTAGATC TGAGCAATAA CAACTATTAC 
TTTCTGGCAG AAGGTGTTAC TCACGTGCTA AGTTTTATGA AAAACCTACC CCACTTGAGG 
AAGCTGATGA TGAATGACAA TGATATTTCC ACCACTATTG ATACAGGAAT GGAAAGTCAA 
TCTCTTCGAA TTTTAGAATT CAGAGGAAAT CACTTAGATG TTCTCTGGAA GGATGGCAAT 
GATAGATACT TGTCATTCTT CAAGAATCTG AGCAGCCTGG AAGAACTGGA TATTTCCTTC 
AACTCACTCA GCTTTTTGCC TCATAGTGTT TTTGAAAAAA TGCCTCCCAG TCTCAAGGTG 
CTCAACCTAA CAAATAATCA GCTGAAGAGT TTCATTTGGG GAAACCTCCC CTCTCTGAAG 
AACCTGGTAA CTCTGGACCT GAGCAATAAC CTTCTGACTA ATGTTCCCCG AGAACTGTCC 
AATTGCACTT CATCGCTGCA GGTACTGATC CTGCGAAACA ATCGCATTCA CGTGCTAACC 
AAACATTTTC TCAGGGGTGC TTTTGAACTG AGATACTTGG ACCTCAGCTC AAATAAGATT 
GAAATAATTA AGAGATCTAG CTTCCCTGAA AATGTCATTA ACAACCTGAA GATGCTGCTT 
TTGCACGGCA ATCCCTTTAA GTGTAACTGT GAGGCTGTGT GGTTTGTCTG GTGGATCAAC 
CGGACGCAGG TGACCATTCC TCTTCTGGCC ACGGACGTCA CCTGTGCTGG CCCAGGGGCA 
CATAAGGGAA GGAGCGTGGT TTTCCTGGAC CTGTACACCT GTGAGCTGGA CACGTCCTAT 
TTGATCCTGT ACGCTCTGTC AGCTTCAGCC ATCCTCGGCT TGGTGGTGTT CACTGTGATG 
AGCCATCTCT ACTTCTGGGA TGTGTGGTAC AGCTACCATT ACTGCGCTGC CAAGCTGAAG 
GGCTATCAGC GCTTGTCGTC ACCAGCCGCT TGCTACGATG CCTTTATTGC CTATGACAGT 
GAAGATCCTG CTGTGAACGA GTGGGTACTG CAAGAACTGG TTGAAAGGCT GGAAAACCAA 
AAAGCCAGGC AGTTCAATTT ATGCCTGGAA GGAAGGGACT GGCTCCCAGG ACAGCCGGTC 
TTTGACAACC TTTCCCAGAG CATTCAGCTG AGCAAAAAGA CCATATTTGT GCTGACCAAC 
AGGTATATTA AAAGTGGCCG CTTCAAGACA ACATTTTATA TGGCTCATCA GCGCCTTCTA 
GATGAAAAAA TGGATGTCAT TATCTTGATA TTTCTTGAGA AGGTTTTGCA GCAGTCCCGC 
TATGTCCGTC TGAGGAAGAG GCTGTGCAGA AGTTCAGTCC TGGAATGGCC AACTAATCCT 
CAGTCTCAGC CCTACTTCTG GCAGTGCCTG AAAAATGCCA TAGCTACGAG CAATTCTCTG 
GCATACAACA AGCTTCTTCA AGAAACTGTT TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TLR7 ( XM_014252883.1 ) cDNA ORF clone, Pseudopodoces humilis(Tibetan ground-tit) -> XP_014108358.1 Pseudopodoces humilis toll-like receptor 7 (TLR7), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OPs05845
Clone ID Related Accession (Same CDS sequence)	XM_014252883.1
Accession Version	XM_014252883.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3126bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-20
Organism	Pseudopodoces humilis(Tibetan ground-tit)
Product	toll-like receptor 7 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005087554.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pseudopodoces humilis Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCAAATG CATTGCCATT TATCTTGCTC TTCATATTCC CAATGCTGCT GTCTGGGGCT 
TGGTTTCCCA AAACTTTACC CTGTGATGTT AAATCTTCAG AAGGTGCTGT GACAGTGGAC 
TGCACTGACC GGCGCCTTAC AGAAGTCCCC AGAGGGATCC CTGGAAATGC TACCAACCTT 
ACCCTGAGTA TTAATCATAT TCCCCATATC TACCCAACAT CCTTCAATCA TCTTGAAAAC 
CTCGAGGAGA TTGACTTCAG ATGCAACTGT GTGCCTGTCA AGCTGGGGCC CAAAAATCAT 
GTGTGCACCA GCCCCCTGAA AATTGAGAAT GGTAGTTTTG CTGCCTTGAC AAGATTGAAG 
GCATTGTACC TGGATGCAAA CCAGCTGGCA GAAGTACCCC GAGGTCTTCC TGCTGCTTTA 
AGCCTGCTGA GTCTGGAAGC AAACAGTATC TTTTCTATCC AAAAAGCCAG CTTCTCGGAG 
CTAGGGAACA TAGAGGTGCT GTATCTTGGA CAGAACTGTT ACTACCGCAA TCCATGCAAT 
GTTTCATTTG AAATTGAGAA AACAGCCTTT CTGGGGCTGA AAAAGTTAAC AATACTATCC 
CTGAAGTCCA ACAACTTAAC ACAAATTCCT CCCAATTTGT CATCTACTTT AAAGGAATTG 
TATATTTACA ACAACATGAT TCAAGAGATT CAAGAGCAGG ATTTAAGTGG TCTTCCCAAC 
CTAGAAATTC TCGACCTAAG TGGCAATTGC CCACGTTGCT ATAATGCCCC ATATCCCTGC 
GTTCCCTGTC CCAAGAGCTC CATTCAGATA CATTCAAATG CTTTTGATTC TTTGGAAAAA 
TTAAGAATTT TGCGGCTTCA CAGTAACTCT CTACAGAGCA TACCCAGCAG CTGGTTTAAA 
AACTTCAGGA ATCTCAAAGA ACTTGACCTC TCCCGAAATT TCCTCATGAG GGAGATTGGA 
GATGCTCAGT TTTTGACATT TATCCCCAGT CTTGTGCAGC TTGATCTGTC CTTTAATTTT 
GAACTGAAGG TGTATTCTCC CTTCTTGGAT CTTTCTGAGA CATTTTCTAC CCTCTCTAAC 
CTGGAAATCC TGAGACTCAA GGGTTATGTC TTTAAAGAGC TGAGGGCACA AGATCTATAT 
CCACTGCTCA GTCTTAGGAA TCTAACTATG TTGGATCTCG GGACTAATTT TATTAAAATT 
GCAAACCTAA CTGTGTTTGA TGAATTCCCA GCTCTTAAGT TCATTGATCT CTCAGTGAAT 
AAAATTTCTC CTTCTTCAGG GGAAGGCAAC CTCTATGGAT TTTGCTCTAA TCCTGCCATT 
TCAGCCGAGC AATACAACAG ACAAGTACAA CAAGAGATGC ATTATTTTAG ATATGATGTG 
TATGAGCGAA GTTGCCGTTC CAAAGACAAA GAGGATTCTT CTTACCAGTC TTTAGTTAAG 
GAAGATTGCC TTAACTATGG AAAAACATTG GATTTAAGCA GAAACAATGT ATTTTTTGTT 
AACCCCTCTG ACTTTCAGGG GCTTAGCTCC CTGAAATGTC TTAACTTGTC AGGTAATGCA 
ATAAGTCAAA CTTTGAATGG AAGTGAATTC TTTTACTTGT CTGGATTGAA ATATCTGGAT 
TTTTCTAACA ACAGGGTTGA TTTGTTATAC CAAACTGCTT TCAAAGAACT AAAATATTTA 
GAAATTCTAG ATCTGAGCAA TAACAACTAT TACTTTCTGG CAGAAGGTGT TACTCACGTG 
CTAAGTTTTA TGAAAAACCT ACCCCACTTG AGGAAGCTGA TGATGAATGA CAATGATATT 
TCCACCACTA TTGATACAGG AATGGAAAGT CAATCTCTTC GAATTTTAGA ATTCAGAGGA 
AATCACTTAG ATGTTCTCTG GAAGGATGGC AATGATAGAT ACTTGTCATT CTTCAAGAAT 
CTGAGCAGCC TGGAAGAACT GGATATTTCC TTCAACTCAC TCAGCTTTTT GCCTCATAGT 
GTTTTTGAAA AAATGCCTCC CAGTCTCAAG GTGCTCAACC TAACAAATAA TCAGCTGAAG 
AGTTTCATTT GGGGAAACCT CCCCTCTCTG AAGAACCTGG TAACTCTGGA CCTGAGCAAT 
AACCTTCTGA CTAATGTTCC CCGAGAACTG TCCAATTGCA CTTCATCGCT GCAGGTACTG 
ATCCTGCGAA ACAATCGCAT TCACGTGCTA ACCAAACATT TTCTCAGGGG TGCTTTTGAA 
CTGAGATACT TGGACCTCAG CTCAAATAAG ATTGAAATAA TTAAGAGATC TAGCTTCCCT 
GAAAATGTCA TTAACAACCT GAAGATGCTG CTTTTGCACG GCAATCCCTT TAAGTGTAAC 
TGTGAGGCTG TGTGGTTTGT CTGGTGGATC AACCGGACGC AGGTGACCAT TCCTCTTCTG 
GCCACGGACG TCACCTGTGC TGGCCCAGGG GCACATAAGG GAAGGAGCGT GGTTTTCCTG 
GACCTGTACA CCTGTGAGCT GGACACGTCC TATTTGATCC TGTACGCTCT GTCAGCTTCA 
GCCATCCTCG GCTTGGTGGT GTTCACTGTG ATGAGCCATC TCTACTTCTG GGATGTGTGG 
TACAGCTACC ATTACTGCGC TGCCAAGCTG AAGGGCTATC AGCGCTTGTC GTCACCAGCC 
GCTTGCTACG ATGCCTTTAT TGCCTATGAC AGTGAAGATC CTGCTGTGAA CGAGTGGGTA 
CTGCAAGAAC TGGTTGAAAG GCTGGAAAAC CAAAAAGCCA GGCAGTTCAA TTTATGCCTG 
GAAGGAAGGG ACTGGCTCCC AGGACAGCCG GTCTTTGACA ACCTTTCCCA GAGCATTCAG 
CTGAGCAAAA AGACCATATT TGTGCTGACC AACAGGTATA TTAAAAGTGG CCGCTTCAAG 
ACAACATTTT ATATGGCTCA TCAGCGCCTT CTAGATGAAA AAATGGATGT CATTATCTTG 
ATATTTCTTG AGAAGGTTTT GCAGCAGTCC CGCTATGTCC GTCTGAGGAA GAGGCTGTGC 
AGAAGTTCAG TCCTGGAATG GCCAACTAAT CCTCAGTCTC AGCCCTACTT CTGGCAGTGC 
CTGAAAAATG CCATAGCTAC GAGCAATTCT CTGGCATACA ACAAGCTTCT TCAAGAAACT 
GTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014108358.1
CDS	174..3299
Translation	MSNALPFILLFIFPMLLSGAWFPKTLPCDVKSSEGAVTVDCTDRRLTEVPRGIPGNATNLTLSINHIPHIYPTSFNHLENLEEIDFRCNCVPVKLGPKNHVCTSPLKIENGSFAALTRLKALYLDANQLAEVPRGLPAALSLLSLEANSIFSIQKASFSELGNIEVLYLGQNCYYRNPCNVSFEIEKTAFLGLKKLTILSLKSNNLTQIPPNLSSTLKELYIYNNMIQEIQEQDLSGLPNLEILDLSGNCPRCYNAPYPCVPCPKSSIQIHSNAFDSLEKLRILRLHSNSLQSIPSSWFKNFRNLKELDLSRNFLMREIGDAQFLTFIPSLVQLDLSFNFELKVYSPFLDLSETFSTLSNLEILRLKGYVFKELRAQDLYPLLSLRNLTMLDLGTNFIKIANLTVFDEFPALKFIDLSVNKISPSSGEGNLYGFCSNPAISAEQYNRQVQQEMHYFRYDVYERSCRSKDKEDSSYQSLVKEDCLNYGKTLDLSRNNVFFVNPSDFQGLSSLKCLNLSGNAISQTLNGSEFFYLSGLKYLDFSNNRVDLLYQTAFKELKYLEILDLSNNNYYFLAEGVTHVLSFMKNLPHLRKLMMNDNDISTTIDTGMESQSLRILEFRGNHLDVLWKDGNDRYLSFFKNLSSLEELDISFNSLSFLPHSVFEKMPPSLKVLNLTNNQLKSFIWGNLPSLKNLVTLDLSNNLLTNVPRELSNCTSSLQVLILRNNRIHVLTKHFLRGAFELRYLDLSSNKIEIIKRSSFPENVINNLKMLLLHGNPFKCNCEAVWFVWWINRTQVTIPLLATDVTCAGPGAHKGRSVVFLDLYTCELDTSYLILYALSASAILGLVVFTVMSHLYFWDVWYSYHYCAAKLKGYQRLSSPAACYDAFIAYDSEDPAVNEWVLQELVERLENQKARQFNLCLEGRDWLPGQPVFDNLSQSIQLSKKTIFVLTNRYIKSGRFKTTFYMAHQRLLDEKMDVIILIFLEKVLQQSRYVRLRKRLCRSSVLEWPTNPQSQPYFWQCLKNAIATSNSLAYNKLLQETV

Target ORF information:

RefSeq Version	XM_014252883.1
Organism	Pseudopodoces humilis(Tibetan ground-tit)
Definition	Pseudopodoces humilis toll-like receptor 7 (TLR7), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014252883.1

ORF Insert Sequence:

ATGTCAAATG CATTGCCATT TATCTTGCTC TTCATATTCC CAATGCTGCT GTCTGGGGCT 
TGGTTTCCCA AAACTTTACC CTGTGATGTT AAATCTTCAG AAGGTGCTGT GACAGTGGAC 
TGCACTGACC GGCGCCTTAC AGAAGTCCCC AGAGGGATCC CTGGAAATGC TACCAACCTT 
ACCCTGAGTA TTAATCATAT TCCCCATATC TACCCAACAT CCTTCAATCA TCTTGAAAAC 
CTCGAGGAGA TTGACTTCAG ATGCAACTGT GTGCCTGTCA AGCTGGGGCC CAAAAATCAT 
GTGTGCACCA GCCCCCTGAA AATTGAGAAT GGTAGTTTTG CTGCCTTGAC AAGATTGAAG 
GCATTGTACC TGGATGCAAA CCAGCTGGCA GAAGTACCCC GAGGTCTTCC TGCTGCTTTA 
AGCCTGCTGA GTCTGGAAGC AAACAGTATC TTTTCTATCC AAAAAGCCAG CTTCTCGGAG 
CTAGGGAACA TAGAGGTGCT GTATCTTGGA CAGAACTGTT ACTACCGCAA TCCATGCAAT 
GTTTCATTTG AAATTGAGAA AACAGCCTTT CTGGGGCTGA AAAAGTTAAC AATACTATCC 
CTGAAGTCCA ACAACTTAAC ACAAATTCCT CCCAATTTGT CATCTACTTT AAAGGAATTG 
TATATTTACA ACAACATGAT TCAAGAGATT CAAGAGCAGG ATTTAAGTGG TCTTCCCAAC 
CTAGAAATTC TCGACCTAAG TGGCAATTGC CCACGTTGCT ATAATGCCCC ATATCCCTGC 
GTTCCCTGTC CCAAGAGCTC CATTCAGATA CATTCAAATG CTTTTGATTC TTTGGAAAAA 
TTAAGAATTT TGCGGCTTCA CAGTAACTCT CTACAGAGCA TACCCAGCAG CTGGTTTAAA 
AACTTCAGGA ATCTCAAAGA ACTTGACCTC TCCCGAAATT TCCTCATGAG GGAGATTGGA 
GATGCTCAGT TTTTGACATT TATCCCCAGT CTTGTGCAGC TTGATCTGTC CTTTAATTTT 
GAACTGAAGG TGTATTCTCC CTTCTTGGAT CTTTCTGAGA CATTTTCTAC CCTCTCTAAC 
CTGGAAATCC TGAGACTCAA GGGTTATGTC TTTAAAGAGC TGAGGGCACA AGATCTATAT 
CCACTGCTCA GTCTTAGGAA TCTAACTATG TTGGATCTCG GGACTAATTT TATTAAAATT 
GCAAACCTAA CTGTGTTTGA TGAATTCCCA GCTCTTAAGT TCATTGATCT CTCAGTGAAT 
AAAATTTCTC CTTCTTCAGG GGAAGGCAAC CTCTATGGAT TTTGCTCTAA TCCTGCCATT 
TCAGCCGAGC AATACAACAG ACAAGTACAA CAAGAGATGC ATTATTTTAG ATATGATGTG 
TATGAGCGAA GTTGCCGTTC CAAAGACAAA GAGGATTCTT CTTACCAGTC TTTAGTTAAG 
GAAGATTGCC TTAACTATGG AAAAACATTG GATTTAAGCA GAAACAATGT ATTTTTTGTT 
AACCCCTCTG ACTTTCAGGG GCTTAGCTCC CTGAAATGTC TTAACTTGTC AGGTAATGCA 
ATAAGTCAAA CTTTGAATGG AAGTGAATTC TTTTACTTGT CTGGATTGAA ATATCTGGAT 
TTTTCTAACA ACAGGGTTGA TTTGTTATAC CAAACTGCTT TCAAAGAACT AAAATATTTA 
GAAATTCTAG ATCTGAGCAA TAACAACTAT TACTTTCTGG CAGAAGGTGT TACTCACGTG 
CTAAGTTTTA TGAAAAACCT ACCCCACTTG AGGAAGCTGA TGATGAATGA CAATGATATT 
TCCACCACTA TTGATACAGG AATGGAAAGT CAATCTCTTC GAATTTTAGA ATTCAGAGGA 
AATCACTTAG ATGTTCTCTG GAAGGATGGC AATGATAGAT ACTTGTCATT CTTCAAGAAT 
CTGAGCAGCC TGGAAGAACT GGATATTTCC TTCAACTCAC TCAGCTTTTT GCCTCATAGT 
GTTTTTGAAA AAATGCCTCC CAGTCTCAAG GTGCTCAACC TAACAAATAA TCAGCTGAAG 
AGTTTCATTT GGGGAAACCT CCCCTCTCTG AAGAACCTGG TAACTCTGGA CCTGAGCAAT 
AACCTTCTGA CTAATGTTCC CCGAGAACTG TCCAATTGCA CTTCATCGCT GCAGGTACTG 
ATCCTGCGAA ACAATCGCAT TCACGTGCTA ACCAAACATT TTCTCAGGGG TGCTTTTGAA 
CTGAGATACT TGGACCTCAG CTCAAATAAG ATTGAAATAA TTAAGAGATC TAGCTTCCCT 
GAAAATGTCA TTAACAACCT GAAGATGCTG CTTTTGCACG GCAATCCCTT TAAGTGTAAC 
TGTGAGGCTG TGTGGTTTGT CTGGTGGATC AACCGGACGC AGGTGACCAT TCCTCTTCTG 
GCCACGGACG TCACCTGTGC TGGCCCAGGG GCACATAAGG GAAGGAGCGT GGTTTTCCTG 
GACCTGTACA CCTGTGAGCT GGACACGTCC TATTTGATCC TGTACGCTCT GTCAGCTTCA 
GCCATCCTCG GCTTGGTGGT GTTCACTGTG ATGAGCCATC TCTACTTCTG GGATGTGTGG 
TACAGCTACC ATTACTGCGC TGCCAAGCTG AAGGGCTATC AGCGCTTGTC GTCACCAGCC 
GCTTGCTACG ATGCCTTTAT TGCCTATGAC AGTGAAGATC CTGCTGTGAA CGAGTGGGTA 
CTGCAAGAAC TGGTTGAAAG GCTGGAAAAC CAAAAAGCCA GGCAGTTCAA TTTATGCCTG 
GAAGGAAGGG ACTGGCTCCC AGGACAGCCG GTCTTTGACA ACCTTTCCCA GAGCATTCAG 
CTGAGCAAAA AGACCATATT TGTGCTGACC AACAGGTATA TTAAAAGTGG CCGCTTCAAG 
ACAACATTTT ATATGGCTCA TCAGCGCCTT CTAGATGAAA AAATGGATGT CATTATCTTG 
ATATTTCTTG AGAAGGTTTT GCAGCAGTCC CGCTATGTCC GTCTGAGGAA GAGGCTGTGC 
AGAAGTTCAG TCCTGGAATG GCCAACTAAT CCTCAGTCTC AGCCCTACTT CTGGCAGTGC 
CTGAAAAATG CCATAGCTAC GAGCAATTCT CTGGCATACA ACAAGCTTCT TCAAGAAACT 
GTTTAG