TIMELESS cDNA ORF clone, Protobothrops mucrosquamatus

Gene
Clones
gRNAs

Summary

Gene Symbol	TIMELESS
Entrez Gene ID	107290925
Full Name	timeless circadian regulator
Gene Type	protein-coding
Organism	Protobothrops mucrosquamatus

mRNA and Protein(s)

mRNA	Protein	Name
XM_015819871.1	XP_015675357.1	protein timeless homolog
XM_029284843.1	XP_029140676.1	protein timeless homolog

Pathways from BioSystems
Gene Ontology

The following TIMELESS gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TIMELESS cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OPr197407	XM_029284843.1 Latest version!	Protobothrops mucrosquamatus timeless circadian regulator (TIMELESS), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OPr23201	XM_015819871.1 Latest version!	Protobothrops mucrosquamatus timeless circadian clock (TIMELESS), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

TIMELESS ( XM_029284843.1 ) cDNA ORF clone, Protobothrops mucrosquamatus -> XP_029140676.1 Protobothrops mucrosquamatus timeless circadian regulator (TIMELESS), mRNA.

Price & Availability↑

CloneID	OPr197407
Clone ID Related Accession (Same CDS sequence)	XM_029284843.1
Accession Version	XM_029284843.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2652bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-05-22
Organism	Protobothrops mucrosquamatus
Product	protein timeless homolog
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015386227.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Protobothrops mucrosquamatus Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACTTGT ACATGATGAA TTGTGAACTC CTAGCAACTT GCAGTGCCCT TGGATTCTTA 
GAAGGGGACA CCTACCATAA AGAACCAGAC TGTATAGAGA GTGTGAAAGA TTTAATCCGC 
TATCTGCGCC ATGAAGATGA GACCAGGGAT ATTCGGCAAC AGCTGGGAGC TGCTCAGATT 
CTGCAAAATG ACCTGATCCC TATTATCGTC CAGTACCCCC AAGATAAACA GCTGTTTGAT 
GCTGTCATCA GATTAATGGT GAATTTAACT CAACCAGCTA TCCTCTGCTT TGGAAAAATT 
CCCCAGGACA TTAACCTCCG CCATCACTTC CTGCAGACTG TCTCTTACCT GCAGGCTTAT 
AAAGAAGCAT TTGCCAGCGA GAAGGTCTTT GGGGTGCTGA GCGAGAAGCT ATATGAATTG 
CTGCAGCTGG ACTGGGAGCA GCGCCAGGAA GAAGATAACC TGTTAATTGA GCGCATCTTG 
CTTCTGGTGC GCAATGTGCT GCATGTGCCA GCTGATCCTA ATGAAGAGAA GAACATTGAT 
GATGATGCCA ACATCCATGA CCGGGTGCTC TGGGCTATGC ACATCAGCGG GATGGATGAT 
TTGCTCAAAT TCTTGGCCAG CTCCACAAGT GAACAGCAGT GGAGTCTGCA CGTGCTGGAG 
ATAATCTCCC TTATGTTCCG TGACCAGAAT GCAGAGCTGT TGGCAGCCAC AGGGCAGACC 
CGTTCAGCCA AGGAGCAGAG CATAGACGTG GCAGGCCTGG AAATCTTGAG GCAGAAGGAA 
CAGGCTGAGA AGAAGACTCG TATATTCCAG CGGAGTAGCA GACACTCTCG TTTTGGTGGC 
TGTTATGTTA TTCAGGGCTT GAAAGCTATT GGGGAAAGAG ATGTGGTGAT GCACAAGGGG 
CTACACAATA TGAAGAACTA CAGCCACGAT CTGTGCAAGG AGGTACGCCG GGTGCCGAAA 
AGGCAGCAGC TCGCCAGTGA TGGTGAAGGA CCCCCTCGCC GCTCTGCCCT AAATGTGCGG 
CGCTTCCTCC AGGAGTTCTG TGTGGAGTTC TTGGAGAACT GCTACAATCG CCTCATGTAC 
CTGGTCAAGG ATCACTTGAT CAGGGAAAAA GCCCAACAGC ACGATGAGAC TTACTACTTA 
TGGGCTATGG CTTTTTTCAT GGCCTTCAAC CGGGCCTCTT CTTTCCAGCC TCAGCTGGTT 
TCTGAGACGG TTGGCGTTCG GACCTTCCAT TTCATTGAAC AGAGTCTGAC CAACTACTAT 
GAAATGAGCC TGGTAGATAA GAAGGACATC ACCTCTTGGT CTAAAAGGAT GCATCTGGCT 
CTCAAGGCTT ACCAAGAATT GCTGACGACT ATGCATGAAA TGGACCATTC CCAGGAAGAA 
GCTGTGCGCA ACAGCAGCCA CATCCTTAAA AATAACATTT TCTACTTGAT GGAATATCGG 
GAGTTGTTCC TCGCTCTTTT CCGTAAGTTT GATGAGAAAA AGCAACCCAG GTCATTCCTG 
AGAGATCTGG TGGAGACAAC TCATCTCTTC TTGAAGATGC TGGAGAAATT TAGCAAAGGG 
AGGAAAAACC TAGTGGTCCA GGGCAAGAAA TGCAGGAGGA AAAAGAAATC CAAGGCTCAT 
TCTGCTGGGC CCGGTGCGCA ACCCCAAACT CCTGAAGAGT TAGAGGAGGT GTGGCCAAAG 
CTGGTGGAGC AAATTGATAA GTGCATAAAG GGTTTTGAGT CTTCTCCAGA GGATGTCATT 
CCTTTTGACG CCACATCAGA AGTTCCATTA GAGGAACAGC GAGCTGAGGC CATGATACGG 
ATCCAGGACA GCTTATTAAC CGGCCAGGCA CCAGAAGCGC TCTCACTCTT GCGCTCGGCA 
AGGGAGGTGT GGCCTGAAGG AGACGTGTTT GGCTCATCCG CTGTTGATCC AGTTGAAGAA 
ATGGAGCTGC TGAAGCAAAT CTTCTTCGCC AACCTCCCTC GGCAAGCAGC GACTGACCCA 
GATGAGCCTG AGGAGGCTGC CGAGGATGAG GAAGTTGCTG AGGAAGAGTT GGCATCTGTG 
CAGGTGTCTG AAAAAGAGTT CAGCTTCCTG GACTATGTGA AGCGGTTTGC TAGTGCTCAT 
GTAGTCAAGG CCTACGTCCT GCTGTTCAGA AATTACCAGC AGAACAGCCC CCACACTAAT 
CACTGCGTGG TCAAGATGTT GCACCGCATC GCCTGTGACG TCAAGATGGA GGCCTTACTC 
TTTCAGCTGT CGGTCTTCTG CCTTTTCAAC CATCTTCTCA GTGACCCTGC CGCTGGAGCT 
TATAAGGAGC TGGTGACCTT TGCCAAATAC GTTGTGGGCA AATTTTTCGC ATTGGCATCG 
ACCAATAAAA AAGCCTACAT GGAACTGTTG TTCTGGAAAA ACACAGCTGT GGTTCGAGAA 
ATGACCGAAG GCTACACGGT GTCACGGGAA GATGGAAGTT CTAATTTGCA CAAGGCCTCC 
CATTGGACTG AAGAGGAGGA GGAAGAACTT CAGGAGCTCT ACCACAAGTA TAAAGAAATG 
GAAGGTGAAG ATGTGCTTGA CAACATCCTG TCACATCTCG GCTCTCATCC CCGGGGACGA 
AAGCAGGTGG CCAGGAAACT TGTGCACTTG GGGTTGGTGA GCAGCATACG AGACTTTCCT 
CAGGAGCGGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_029140676.1
CDS	64..2715
Translation	MDLYMMNCELLATCSALGFLEGDTYHKEPDCIESVKDLIRYLRHEDETRDIRQQLGAAQILQNDLIPIIVQYPQDKQLFDAVIRLMVNLTQPAILCFGKIPQDINLRHHFLQTVSYLQAYKEAFASEKVFGVLSEKLYELLQLDWEQRQEEDNLLIERILLLVRNVLHVPADPNEEKNIDDDANIHDRVLWAMHISGMDDLLKFLASSTSEQQWSLHVLEIISLMFRDQNAELLAATGQTRSAKEQSIDVAGLEILRQKEQAEKKTRIFQRSSRHSRFGGCYVIQGLKAIGERDVVMHKGLHNMKNYSHDLCKEVRRVPKRQQLASDGEGPPRRSALNVRRFLQEFCVEFLENCYNRLMYLVKDHLIREKAQQHDETYYLWAMAFFMAFNRASSFQPQLVSETVGVRTFHFIEQSLTNYYEMSLVDKKDITSWSKRMHLALKAYQELLTTMHEMDHSQEEAVRNSSHILKNNIFYLMEYRELFLALFRKFDEKKQPRSFLRDLVETTHLFLKMLEKFSKGRKNLVVQGKKCRRKKKSKAHSAGPGAQPQTPEELEEVWPKLVEQIDKCIKGFESSPEDVIPFDATSEVPLEEQRAEAMIRIQDSLLTGQAPEALSLLRSAREVWPEGDVFGSSAVDPVEEMELLKQIFFANLPRQAATDPDEPEEAAEDEEVAEEELASVQVSEKEFSFLDYVKRFASAHVVKAYVLLFRNYQQNSPHTNHCVVKMLHRIACDVKMEALLFQLSVFCLFNHLLSDPAAGAYKELVTFAKYVVGKFFALASTNKKAYMELLFWKNTAVVREMTEGYTVSREDGSSNLHKASHWTEEEEEELQELYHKYKEMEGEDVLDNILSHLGSHPRGRKQVARKLVHLGLVSSIRDFPQER

Target ORF information:

RefSeq Version	XM_029284843.1
Organism	Protobothrops mucrosquamatus
Definition	Protobothrops mucrosquamatus timeless circadian regulator (TIMELESS), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_029284843.1

ORF Insert Sequence:

ATGGACTTGT ACATGATGAA TTGTGAACTC CTAGCAACTT GCAGTGCCCT TGGATTCTTA 
GAAGGGGACA CCTACCATAA AGAACCAGAC TGTATAGAGA GTGTGAAAGA TTTAATCCGC 
TATCTGCGCC ATGAAGATGA GACCAGGGAT ATTCGGCAAC AGCTGGGAGC TGCTCAGATT 
CTGCAAAATG ACCTGATCCC TATTATCGTC CAGTACCCCC AAGATAAACA GCTGTTTGAT 
GCTGTCATCA GATTAATGGT GAATTTAACT CAACCAGCTA TCCTCTGCTT TGGAAAAATT 
CCCCAGGACA TTAACCTCCG CCATCACTTC CTGCAGACTG TCTCTTACCT GCAGGCTTAT 
AAAGAAGCAT TTGCCAGCGA GAAGGTCTTT GGGGTGCTGA GCGAGAAGCT ATATGAATTG 
CTGCAGCTGG ACTGGGAGCA GCGCCAGGAA GAAGATAACC TGTTAATTGA GCGCATCTTG 
CTTCTGGTGC GCAATGTGCT GCATGTGCCA GCTGATCCTA ATGAAGAGAA GAACATTGAT 
GATGATGCCA ACATCCATGA CCGGGTGCTC TGGGCTATGC ACATCAGCGG GATGGATGAT 
TTGCTCAAAT TCTTGGCCAG CTCCACAAGT GAACAGCAGT GGAGTCTGCA CGTGCTGGAG 
ATAATCTCCC TTATGTTCCG TGACCAGAAT GCAGAGCTGT TGGCAGCCAC AGGGCAGACC 
CGTTCAGCCA AGGAGCAGAG CATAGACGTG GCAGGCCTGG AAATCTTGAG GCAGAAGGAA 
CAGGCTGAGA AGAAGACTCG TATATTCCAG CGGAGTAGCA GACACTCTCG TTTTGGTGGC 
TGTTATGTTA TTCAGGGCTT GAAAGCTATT GGGGAAAGAG ATGTGGTGAT GCACAAGGGG 
CTACACAATA TGAAGAACTA CAGCCACGAT CTGTGCAAGG AGGTACGCCG GGTGCCGAAA 
AGGCAGCAGC TCGCCAGTGA TGGTGAAGGA CCCCCTCGCC GCTCTGCCCT AAATGTGCGG 
CGCTTCCTCC AGGAGTTCTG TGTGGAGTTC TTGGAGAACT GCTACAATCG CCTCATGTAC 
CTGGTCAAGG ATCACTTGAT CAGGGAAAAA GCCCAACAGC ACGATGAGAC TTACTACTTA 
TGGGCTATGG CTTTTTTCAT GGCCTTCAAC CGGGCCTCTT CTTTCCAGCC TCAGCTGGTT 
TCTGAGACGG TTGGCGTTCG GACCTTCCAT TTCATTGAAC AGAGTCTGAC CAACTACTAT 
GAAATGAGCC TGGTAGATAA GAAGGACATC ACCTCTTGGT CTAAAAGGAT GCATCTGGCT 
CTCAAGGCTT ACCAAGAATT GCTGACGACT ATGCATGAAA TGGACCATTC CCAGGAAGAA 
GCTGTGCGCA ACAGCAGCCA CATCCTTAAA AATAACATTT TCTACTTGAT GGAATATCGG 
GAGTTGTTCC TCGCTCTTTT CCGTAAGTTT GATGAGAAAA AGCAACCCAG GTCATTCCTG 
AGAGATCTGG TGGAGACAAC TCATCTCTTC TTGAAGATGC TGGAGAAATT TAGCAAAGGG 
AGGAAAAACC TAGTGGTCCA GGGCAAGAAA TGCAGGAGGA AAAAGAAATC CAAGGCTCAT 
TCTGCTGGGC CCGGTGCGCA ACCCCAAACT CCTGAAGAGT TAGAGGAGGT GTGGCCAAAG 
CTGGTGGAGC AAATTGATAA GTGCATAAAG GGTTTTGAGT CTTCTCCAGA GGATGTCATT 
CCTTTTGACG CCACATCAGA AGTTCCATTA GAGGAACAGC GAGCTGAGGC CATGATACGG 
ATCCAGGACA GCTTATTAAC CGGCCAGGCA CCAGAAGCGC TCTCACTCTT GCGCTCGGCA 
AGGGAGGTGT GGCCTGAAGG AGACGTGTTT GGCTCATCCG CTGTTGATCC AGTTGAAGAA 
ATGGAGCTGC TGAAGCAAAT CTTCTTCGCC AACCTCCCTC GGCAAGCAGC GACTGACCCA 
GATGAGCCTG AGGAGGCTGC CGAGGATGAG GAAGTTGCTG AGGAAGAGTT GGCATCTGTG 
CAGGTGTCTG AAAAAGAGTT CAGCTTCCTG GACTATGTGA AGCGGTTTGC TAGTGCTCAT 
GTAGTCAAGG CCTACGTCCT GCTGTTCAGA AATTACCAGC AGAACAGCCC CCACACTAAT 
CACTGCGTGG TCAAGATGTT GCACCGCATC GCCTGTGACG TCAAGATGGA GGCCTTACTC 
TTTCAGCTGT CGGTCTTCTG CCTTTTCAAC CATCTTCTCA GTGACCCTGC CGCTGGAGCT 
TATAAGGAGC TGGTGACCTT TGCCAAATAC GTTGTGGGCA AATTTTTCGC ATTGGCATCG 
ACCAATAAAA AAGCCTACAT GGAACTGTTG TTCTGGAAAA ACACAGCTGT GGTTCGAGAA 
ATGACCGAAG GCTACACGGT GTCACGGGAA GATGGAAGTT CTAATTTGCA CAAGGCCTCC 
CATTGGACTG AAGAGGAGGA GGAAGAACTT CAGGAGCTCT ACCACAAGTA TAAAGAAATG 
GAAGGTGAAG ATGTGCTTGA CAACATCCTG TCACATCTCG GCTCTCATCC CCGGGGACGA 
AAGCAGGTGG CCAGGAAACT TGTGCACTTG GGGTTGGTGA GCAGCATACG AGACTTTCCT 
CAGGAGCGGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TIMELESS ( XM_015819871.1 ) cDNA ORF clone, Protobothrops mucrosquamatus -> XP_015675357.1 Protobothrops mucrosquamatus timeless circadian clock (TIMELESS), mRNA.

Price & Availability↑

CloneID	OPr23201
Clone ID Related Accession (Same CDS sequence)	XM_015819871.1
Accession Version	XM_015819871.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3591bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-03-01
Organism	Protobothrops mucrosquamatus
Product	protein timeless homolog
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015386227.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Protobothrops mucrosquamatus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACTTGT ACATGATGAA TTGTGAACTC CTAGCAACTT GCAGTGCCCT TGGATTCTTA 
GAAGGGGACA CCTACCATAA AGAACCAGAC TGTATAGAGA GTGTGAAAGA TTTAATCCGC 
TATCTGCGCC ATGAAGATGA GACCAGGGAT ATTCGGCAAC AGCTGGGAGC TGCTCAGATT 
CTGCAAAATG ACCTGATCCC TATTATCGTC CAGTACCCCC AAGATAAACA GCTGTTTGAT 
GCTGTCATCA GATTAATGGT GAATTTAACT CAACCAGCTA TCCTCTGCTT TGGAAAAATT 
CCCCAGGACA TTAACCTCCG CCATCACTTC CTGCAGACTG TCTCTTACCT GCAGGCTTAT 
AAAGAAGCAT TTGCCAGCGA GAAGGTCTTT GGGGTGCTGA GCGAGAAGCT ATATGAATTG 
CTGCAGCTGG ACTGGGAGCA GCGCCAGGAA GAAGATAACC TGTTAATTGA GCGCATCTTG 
CTTCTGGTGC GCAATGTGCT GCATGTGCCA GCTGATCCTA ATGAAGAGAA GAACATTGAT 
GATGATGCCA ACATCCATGA CCGGGTGCTC TGGGCTATGC ACATCAGCGG GATGGATGAT 
TTGCTCAAAT TCTTGGCCAG CTCCACAAGT GAACAGCAGT GGAGTCTGCA CGTGCTGGAG 
ATAATCTCCC TTATGTTCCG TGACCAGAAT GCAGAGCTGT TGGCAGCCAC AGGGCAGACC 
CGTTCAGCCA AGGAGCAGAG CATAGACGTG GCAGGCCTGG AAATCTTGAG GCAGAAGGAA 
CAGGCTGAGA AGAAGACTCG TATATTCCAG CGGAGTAGCA GACACTCTCG TTTTGGTGGC 
TGTTATGTTA TTCAGGGCTT GAAAGCTATT GGGGAAAGAG ATGTGGTGAT GCACAAGGGG 
CTACACAATA TGAAGAACTA CAGCCACGAT CTGTGCAAGG AGGTACGCCG GGTGCCGAAA 
AGGCAGCAGC TCGCCAGTGA TGGTGAAGGA CCCCCTCGCC GCTCTGCCCT AAATGTGCGG 
CGCTTCCTCC AGGAGTTCTG TGTGGAGTTC TTGGAGAACT GCTACAATCG CCTCATGTAC 
CTGGTCAAGG ATCACTTGAT CAGGGAAAAA GCCCAACAGC ACGATGAGAC TTACTACTTA 
TGGGCTATGG CTTTTTTCAT GGCCTTCAAC CGGGCCTCTT CTTTCCAGCC TCAGCTGGTT 
TCTGAGACGG TTGGCGTTCG GACCTTCCAT TTCATTGAAC AGAGTCTGAC CAACTACTAT 
GAAATGAGCC TGGTAGATAA GAAGGACATC ACCTCTTGGT CTAAAAGGAT GCATCTGGCT 
CTCAAGGCTT ACCAAGAATT GCTGACGACT ATGCATGAAA TGGACCATTC CCAGGAAGAA 
GCTGTGCGCA ACAGCAGCCA CATCCTTAAA AATAACATTT TCTACTTGAT GGAATATCGG 
GAGTTGTTCC TCGCTCTTTT CCGTAAGTTT GATGAGAAAA AGCAACCCAG GTCATTCCTG 
AGAGATCTGG TGGAGACAAC TCATCTCTTC TTGAAGATGC TGGAGAAATT TAGCAAAGGG 
AGGAAAAACC TAGTGGTCCA GGGCAAGAAA TGCAGGAGGA AAAAGAAATC CAAGGCTCAT 
TCTGCTGGGC CCGGTGCGCA ACCCCAAACT CCTGAAGAGT TAGAGGAGGT GTGGCCAAAG 
CTGGTGGAGC AAATTGATAA GTGCATAAAG GGTTTTGAGT CTTCTCCAGA GGATGTCATT 
CCTTTTGACG CCACATCAGA AGTTCCATTA GAGGAACAGC GAGCTGAGGC CATGATACGG 
ATCCAGGACA GCTTATTAAC CGGCCAGGCA CCAGAAGCGC TCTCACTCTT GCGCTCGGCA 
AGGGAGGTGT GGCCTGAAGG AGACGTGTTT GGCTCATCCG CTGTTGATCC AGTTGAAGAA 
ATGGAGCTGC TGAAGCAAAT CTTCTTCGCC AACCTCCCTC GGCAAGCAGC GACTGACCCA 
GATGAGCCTG AGGAGGCTGC CGAGGATGAG GAAGTTGCTG AGGAAGAGTT GGCATCTGTG 
CAGGTGTCTG AAAAAGAGTT CAGCTTCCTG GACTATGTGA AGCGGTTTGC TAGTGCTCAT 
GTAGTCAAGG CCTACGTCCT GCTGTTCAGA AATTACCAGC AGAACAGCCC CCACACTAAT 
CACTGCGTGG TCAAGATGTT GCACCGCATC GCCTGTGACG TCAAGATGGA GGCCTTACTC 
TTTCAGCTGT CGGTCTTCTG CCTTTTCAAC CATCTTCTCA GTGACCCTGC CGCTGGAGCT 
TATAAGGAGC TGGTGACCTT TGCCAAATAC GTTGTGGGCA AATTTTTCGC ATTGGCATCG 
ACCAATAAAA AAGCCTACAT GGAACTGTTG TTCTGGAAAA ACACAGCTGT GGTTCGAGAA 
ATGACCGAAG GCTACACGGT GTCACGGGAA GATGGAAGTT CTAATTTGCA CAAGGCCTCC 
CATTGGACTG AAGAGGAGGA GGAAGAACTT CAGGAGCTCT ACCACAAGTA TAAAGAAATG 
GAAGGTGAAG ATGTGCTTGA CAACATCCTG TCACATCTCG GCTCTCATCC CCGGGGACGA 
AAGCAGGTGG CCAGGAAACT TGTGCACTTG GGGTTGGTGA GCAGCATACG AGACTTTCCT 
CAGGAGCGGA AAGGGACCCG CATTGTGCTA TGGACAGAGG ATCAGGAGCT GGAGCTTTGC 
CGGCTCTTTG AGGAGTTCCG CGACTCCGAC GATATCCTAT CCAATGTAAT GAAGCACATC 
ACAGCGAAAC GATCCAAAGC CCACATTATT GAGAAGATGC TTAGCATAGG GCTGGTGTCT 
GATCGCAAGG AACTTTACAA GAAGCGCAAG CGCAAAGCTG GTTCCCTTAG AGCGAATAAA 
GCCTCGTTCC CCAACGATTC TGAGGTGCCT GACCTGGCTG AGATAGATGA AGAAGAAGAA 
GAAGAAGAAG AAGAAGAAGA AGAAGAAGAA GAACTGGCCA AGGAGAATCC TGGAAATGAC 
AAGGATCAAA AATCCTGGGA CGGGTGGTCT AAGAAAATGT GGGGAAGCGC TTCAGATCGA 
AAACTTGTAC AAAGCCTTAG ATTAGAAGGT TTTGCTGGCC CCTTGCTCTG GCTTCAGAAC 
TGCTTGAACA GAACAGCTGC TGCCCGAGGA GAGGATGGAT GTGCCCAGGC TGTTCCTTTG 
GTGCCATTGA CTGAAGAAAG TGAGGATGCC ATGGAGCACA AAAATTTCCA GAAACTGTTG 
TGGAAGTTGG GGGTGCGATC TCCAGCCAAT GAGCAGGAAT CCTTCTGGCG CATCCCAGCT 
AAGCTCAGCT CAGAACAATT ACGCCACATG GCTGCTTTCA TTGCTTCGGA AGAAATGGAG 
GGGGAGACTC AAGAACAGCA AGCTGAAGAT CATTCAGAAG ATCATCCTGA AGTAATGGAA 
CCAAGAGGGC TGAAAGCAGA AACAGAGGAG CATATCCAGC ATCCTTCAGG CCCTAAAAGA 
AGCCGCTTGG TCGATGAAGA GGAAAACCAT GACCAAGATT CCAGTGATGA GGATGGACCC 
TGTCCTTCCA GTAAGAGGAA GTGCCTGCGA ATTGAAGAAG ACGAGGACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015675357.1
CDS	64..3654
Translation	MDLYMMNCELLATCSALGFLEGDTYHKEPDCIESVKDLIRYLRHEDETRDIRQQLGAAQILQNDLIPIIVQYPQDKQLFDAVIRLMVNLTQPAILCFGKIPQDINLRHHFLQTVSYLQAYKEAFASEKVFGVLSEKLYELLQLDWEQRQEEDNLLIERILLLVRNVLHVPADPNEEKNIDDDANIHDRVLWAMHISGMDDLLKFLASSTSEQQWSLHVLEIISLMFRDQNAELLAATGQTRSAKEQSIDVAGLEILRQKEQAEKKTRIFQRSSRHSRFGGCYVIQGLKAIGERDVVMHKGLHNMKNYSHDLCKEVRRVPKRQQLASDGEGPPRRSALNVRRFLQEFCVEFLENCYNRLMYLVKDHLIREKAQQHDETYYLWAMAFFMAFNRASSFQPQLVSETVGVRTFHFIEQSLTNYYEMSLVDKKDITSWSKRMHLALKAYQELLTTMHEMDHSQEEAVRNSSHILKNNIFYLMEYRELFLALFRKFDEKKQPRSFLRDLVETTHLFLKMLEKFSKGRKNLVVQGKKCRRKKKSKAHSAGPGAQPQTPEELEEVWPKLVEQIDKCIKGFESSPEDVIPFDATSEVPLEEQRAEAMIRIQDSLLTGQAPEALSLLRSAREVWPEGDVFGSSAVDPVEEMELLKQIFFANLPRQAATDPDEPEEAAEDEEVAEEELASVQVSEKEFSFLDYVKRFASAHVVKAYVLLFRNYQQNSPHTNHCVVKMLHRIACDVKMEALLFQLSVFCLFNHLLSDPAAGAYKELVTFAKYVVGKFFALASTNKKAYMELLFWKNTAVVREMTEGYTVSREDGSSNLHKASHWTEEEEEELQELYHKYKEMEGEDVLDNILSHLGSHPRGRKQVARKLVHLGLVSSIRDFPQERKGTRIVLWTEDQELELCRLFEEFRDSDDILSNVMKHITAKRSKAHIIEKMLSIGLVSDRKELYKKRKRKAGSLRANKASFPNDSEVPDLAEIDEEEEEEEEEEEEEEELAKENPGNDKDQKSWDGWSKKMWGSASDRKLVQSLRLEGFAGPLLWLQNCLNRTAAARGEDGCAQAVPLVPLTEESEDAMEHKNFQKLLWKLGVRSPANEQESFWRIPAKLSSEQLRHMAAFIASEEMEGETQEQQAEDHSEDHPEVMEPRGLKAETEEHIQHPSGPKRSRLVDEEENHDQDSSDEDGPCPSSKRKCLRIEEDED

Target ORF information:

RefSeq Version	XM_015819871.1
Organism	Protobothrops mucrosquamatus
Definition	Protobothrops mucrosquamatus timeless circadian clock (TIMELESS), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015819871.1

ORF Insert Sequence:

ATGGACTTGT ACATGATGAA TTGTGAACTC CTAGCAACTT GCAGTGCCCT TGGATTCTTA 
GAAGGGGACA CCTACCATAA AGAACCAGAC TGTATAGAGA GTGTGAAAGA TTTAATCCGC 
TATCTGCGCC ATGAAGATGA GACCAGGGAT ATTCGGCAAC AGCTGGGAGC TGCTCAGATT 
CTGCAAAATG ACCTGATCCC TATTATCGTC CAGTACCCCC AAGATAAACA GCTGTTTGAT 
GCTGTCATCA GATTAATGGT GAATTTAACT CAACCAGCTA TCCTCTGCTT TGGAAAAATT 
CCCCAGGACA TTAACCTCCG CCATCACTTC CTGCAGACTG TCTCTTACCT GCAGGCTTAT 
AAAGAAGCAT TTGCCAGCGA GAAGGTCTTT GGGGTGCTGA GCGAGAAGCT ATATGAATTG 
CTGCAGCTGG ACTGGGAGCA GCGCCAGGAA GAAGATAACC TGTTAATTGA GCGCATCTTG 
CTTCTGGTGC GCAATGTGCT GCATGTGCCA GCTGATCCTA ATGAAGAGAA GAACATTGAT 
GATGATGCCA ACATCCATGA CCGGGTGCTC TGGGCTATGC ACATCAGCGG GATGGATGAT 
TTGCTCAAAT TCTTGGCCAG CTCCACAAGT GAACAGCAGT GGAGTCTGCA CGTGCTGGAG 
ATAATCTCCC TTATGTTCCG TGACCAGAAT GCAGAGCTGT TGGCAGCCAC AGGGCAGACC 
CGTTCAGCCA AGGAGCAGAG CATAGACGTG GCAGGCCTGG AAATCTTGAG GCAGAAGGAA 
CAGGCTGAGA AGAAGACTCG TATATTCCAG CGGAGTAGCA GACACTCTCG TTTTGGTGGC 
TGTTATGTTA TTCAGGGCTT GAAAGCTATT GGGGAAAGAG ATGTGGTGAT GCACAAGGGG 
CTACACAATA TGAAGAACTA CAGCCACGAT CTGTGCAAGG AGGTACGCCG GGTGCCGAAA 
AGGCAGCAGC TCGCCAGTGA TGGTGAAGGA CCCCCTCGCC GCTCTGCCCT AAATGTGCGG 
CGCTTCCTCC AGGAGTTCTG TGTGGAGTTC TTGGAGAACT GCTACAATCG CCTCATGTAC 
CTGGTCAAGG ATCACTTGAT CAGGGAAAAA GCCCAACAGC ACGATGAGAC TTACTACTTA 
TGGGCTATGG CTTTTTTCAT GGCCTTCAAC CGGGCCTCTT CTTTCCAGCC TCAGCTGGTT 
TCTGAGACGG TTGGCGTTCG GACCTTCCAT TTCATTGAAC AGAGTCTGAC CAACTACTAT 
GAAATGAGCC TGGTAGATAA GAAGGACATC ACCTCTTGGT CTAAAAGGAT GCATCTGGCT 
CTCAAGGCTT ACCAAGAATT GCTGACGACT ATGCATGAAA TGGACCATTC CCAGGAAGAA 
GCTGTGCGCA ACAGCAGCCA CATCCTTAAA AATAACATTT TCTACTTGAT GGAATATCGG 
GAGTTGTTCC TCGCTCTTTT CCGTAAGTTT GATGAGAAAA AGCAACCCAG GTCATTCCTG 
AGAGATCTGG TGGAGACAAC TCATCTCTTC TTGAAGATGC TGGAGAAATT TAGCAAAGGG 
AGGAAAAACC TAGTGGTCCA GGGCAAGAAA TGCAGGAGGA AAAAGAAATC CAAGGCTCAT 
TCTGCTGGGC CCGGTGCGCA ACCCCAAACT CCTGAAGAGT TAGAGGAGGT GTGGCCAAAG 
CTGGTGGAGC AAATTGATAA GTGCATAAAG GGTTTTGAGT CTTCTCCAGA GGATGTCATT 
CCTTTTGACG CCACATCAGA AGTTCCATTA GAGGAACAGC GAGCTGAGGC CATGATACGG 
ATCCAGGACA GCTTATTAAC CGGCCAGGCA CCAGAAGCGC TCTCACTCTT GCGCTCGGCA 
AGGGAGGTGT GGCCTGAAGG AGACGTGTTT GGCTCATCCG CTGTTGATCC AGTTGAAGAA 
ATGGAGCTGC TGAAGCAAAT CTTCTTCGCC AACCTCCCTC GGCAAGCAGC GACTGACCCA 
GATGAGCCTG AGGAGGCTGC CGAGGATGAG GAAGTTGCTG AGGAAGAGTT GGCATCTGTG 
CAGGTGTCTG AAAAAGAGTT CAGCTTCCTG GACTATGTGA AGCGGTTTGC TAGTGCTCAT 
GTAGTCAAGG CCTACGTCCT GCTGTTCAGA AATTACCAGC AGAACAGCCC CCACACTAAT 
CACTGCGTGG TCAAGATGTT GCACCGCATC GCCTGTGACG TCAAGATGGA GGCCTTACTC 
TTTCAGCTGT CGGTCTTCTG CCTTTTCAAC CATCTTCTCA GTGACCCTGC CGCTGGAGCT 
TATAAGGAGC TGGTGACCTT TGCCAAATAC GTTGTGGGCA AATTTTTCGC ATTGGCATCG 
ACCAATAAAA AAGCCTACAT GGAACTGTTG TTCTGGAAAA ACACAGCTGT GGTTCGAGAA 
ATGACCGAAG GCTACACGGT GTCACGGGAA GATGGAAGTT CTAATTTGCA CAAGGCCTCC 
CATTGGACTG AAGAGGAGGA GGAAGAACTT CAGGAGCTCT ACCACAAGTA TAAAGAAATG 
GAAGGTGAAG ATGTGCTTGA CAACATCCTG TCACATCTCG GCTCTCATCC CCGGGGACGA 
AAGCAGGTGG CCAGGAAACT TGTGCACTTG GGGTTGGTGA GCAGCATACG AGACTTTCCT 
CAGGAGCGGA AAGGGACCCG CATTGTGCTA TGGACAGAGG ATCAGGAGCT GGAGCTTTGC 
CGGCTCTTTG AGGAGTTCCG CGACTCCGAC GATATCCTAT CCAATGTAAT GAAGCACATC 
ACAGCGAAAC GATCCAAAGC CCACATTATT GAGAAGATGC TTAGCATAGG GCTGGTGTCT 
GATCGCAAGG AACTTTACAA GAAGCGCAAG CGCAAAGCTG GTTCCCTTAG AGCGAATAAA 
GCCTCGTTCC CCAACGATTC TGAGGTGCCT GACCTGGCTG AGATAGATGA AGAAGAAGAA 
GAAGAAGAAG AAGAAGAAGA AGAAGAAGAA GAACTGGCCA AGGAGAATCC TGGAAATGAC 
AAGGATCAAA AATCCTGGGA CGGGTGGTCT AAGAAAATGT GGGGAAGCGC TTCAGATCGA 
AAACTTGTAC AAAGCCTTAG ATTAGAAGGT TTTGCTGGCC CCTTGCTCTG GCTTCAGAAC 
TGCTTGAACA GAACAGCTGC TGCCCGAGGA GAGGATGGAT GTGCCCAGGC TGTTCCTTTG 
GTGCCATTGA CTGAAGAAAG TGAGGATGCC ATGGAGCACA AAAATTTCCA GAAACTGTTG 
TGGAAGTTGG GGGTGCGATC TCCAGCCAAT GAGCAGGAAT CCTTCTGGCG CATCCCAGCT 
AAGCTCAGCT CAGAACAATT ACGCCACATG GCTGCTTTCA TTGCTTCGGA AGAAATGGAG 
GGGGAGACTC AAGAACAGCA AGCTGAAGAT CATTCAGAAG ATCATCCTGA AGTAATGGAA 
CCAAGAGGGC TGAAAGCAGA AACAGAGGAG CATATCCAGC ATCCTTCAGG CCCTAAAAGA 
AGCCGCTTGG TCGATGAAGA GGAAAACCAT GACCAAGATT CCAGTGATGA GGATGGACCC 
TGTCCTTCCA GTAAGAGGAA GTGCCTGCGA ATTGAAGAAG ACGAGGACTG A