VPS50 cDNA ORF clone, Protobothrops mucrosquamatus

Gene
Clones
gRNAs

Summary

Gene Symbol	VPS50
Entrez Gene ID	107283643
Full Name	VPS50, EARP/GARPII complex subunit
Gene Type	protein-coding
Organism	Protobothrops mucrosquamatus

mRNA and Protein(s)

mRNA	Protein	Name
XM_015811571.1	XP_015667057.1	syndetin

Pathways from BioSystems
Gene Ontology

The following VPS50 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the VPS50 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OPr26548	XM_015811571.1 Latest version!	Protobothrops mucrosquamatus VPS50 subunit of EARP/GARPII complex (VPS50), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

VPS50 ( XM_015811571.1 ) cDNA ORF clone, Protobothrops mucrosquamatus -> XP_015667057.1 Protobothrops mucrosquamatus VPS50 subunit of EARP/GARPII complex (VPS50), mRNA.

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CloneID	OPr26548
Clone ID Related Accession (Same CDS sequence)	XM_015811571.1
Accession Version	XM_015811571.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2895bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-05-22
Organism	Protobothrops mucrosquamatus
Product	syndetin
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015386427.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Protobothrops mucrosquamatus Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGAAAA TCAAGTCTCT GATGACCCGA CAGGGTCTTA AAAGTCCTCA GGAGAGTATT 
CATGATTTGA GCCCCCTTGA GAACTTCAGA ATTCCAAGCA AGGAAGAACT TAGAGAACTC 
CGGGAGCAAC CAATTGATCC TCAAGCACAG CAGGAAATAA TTAACAACAT TGAAGAAATC 
TATTTTTCCA ATGATTCCTT TGATATTGTT AAGTATGAGT TAGAGAAGCT GCCAACTGTT 
CTTAGTCTTC AGGAACTGGA AGAGTATAGA GACAAATTAA AGCAACAGCA AGCAGCTGTA 
TCCAAAAAAG TTGCAGACTT GATCCTTGAG AAACAGCCTG CATATGTCAA GGAACTTGAA 
CGAGTTACAT CATTACAAAC AAGCCTTCAG TTAGCAGCTG TTATCTGCAC AAATGGAAGA 
CGGCATTTGA ATACAGCCAA GGAGGGCTTT ACTCAAGCCA GCTTGGGTCT TCTTGCAAAT 
CAACGGAAAC GCCAGTTACT GGTTGGGCTG CTAAAATCTC TAAGAACAAT AAAAACCCTG 
CAACAGACTG ATGTGCGTTT GAGTGAAATG TTGGAGGAGG AAGATTATCC AGGGGCTATA 
CAATTGTGCC TGGAGTGCCA GAAGGCAGCT AGCACTTTCA AACACTACAG TTGCATAAGT 
GAATTGAATT CAAAATTACA GGACACCTTA GAGCAAATTG AGGAGCAGTT AGATGTTGCA 
CTTTCTAAAA TATGCAAGAA CTTTGATATA AACCATTATA CTAAGGTGCA GCAGGCATAC 
AGGCTACTTG GAAAAACACA GACAGCAATG GACCAACTGC ATATGCACTT TACTCAAGCC 
ATTCACAATA CAGTTTTCCA GGTTGTCCTT GGATATGTTG AATTGTGTGC AGGAAACACC 
GACACCAAAT TTCAGAAGCT TCAGTATAAG GATCTTTGCA CACACATTAC ATCAGACAGT 
TACATTCCAT GCCTTGCTGA TCTGTGCAAA GCTCTATGGG AGGTCATGCT GAGTTATTAT 
CGGACAATGG ACTGGCATGA AAAATATGAC CATGGGGAAT CTCCTTCATC TTCTGATGGA 
AATAATATCC TGGATACTGA GGAGACCAAT TTTGATCGTA GCTATGTGAA AAAAAAATTG 
GAGCATGGGC TTTCACGTAT ATGGCAGGAT GTCCAATTGA AAGTGAAGAC CTATTTGCTA 
GGCACCGATA TGTCTAACTT CAAATATGAT GACTTCATAT TTGTACTGGA TATTATCAGC 
AGACTGGTGC AGGTTGGCGA AGAATTTTGT GGCAGCAAGT CAGAAGTCTT GCAAGATTCC 
ATTAGAAAAC AAAGTGTTAA TTATTTCAAG AATTATCACA GAACTCGTCT TGAAGAACTG 
CGAATGTTCT TAGAGAATGA GACTTGGGAA CTTTGTCCTG TTAAATCAAG TTTCAGCATA 
TTGCAACTTC ATGAATTTAA GTTTATGGCA CAATCACGTT CCCCTACTGT GTCACCGAGC 
AAGCAGCCTA CGTTAGAAAC AGAAGGAGAT CTAACTCTTT TTGAGCAGTA TCGTAATGGA 
GGAAATCCCT TTGAAATCCA AGCCAACAAC AAGGAGGAAG AGACAGAAGA TGTGTTGGCT 
TCCAATGGGT ATGAATCGGA TGAACAAGAA AAGAGCGCCT ATCAAGAGTA TGATAGTGAC 
AGTGATGTTC CTGAAGAACT GAAAAGGGAC TATGTTGATG AACAGACTGG AGATGCCCCA 
GTAAAAAGTG TTTCTCGAGA AACTCTGAGG ACCAGGAAGA GATCAGATTA TTGCCTCAAC 
AAGGTGAATG CCCCGATTTT AACCAACACT ACACTTAATG TAATTAGGCT TGTTGGTAAA 
TACATGCAAA TGATGAACAT TTTGAAGCCA ATTGCATTTG ATGTGATTCA CTTTATGTCG 
CAGCTTTTTG ATTACTACAT GTATGCAATA TATACATTTT TTGGCCGAAA TAACACATTT 
GAATCAACAG GACTTGGACT TAGCAGCAGT AGGCTTCGTA CTACCTTGAA TAGAATCCAG 
GAAAGCTTGA TCGATTTGGA TGTCTCCTCT GATGCTGCTG GAACACTAAC TGCTGTTGAA 
GAAAGAAAGG AAAAGGTTCC AATGCCACAT CTAAGTCATC TTGTAATCTT GACATCCAGC 
AACAGTCTCT ATGGCTTGGC AGAGAGAGTG GTAGCCACAG AATCCTTAGT ATTTTTGGCT 
GAACAATTTG AGTTCCTCCA GTCCCATCTT GATGCTGTAA TGCCTCCAGC CAAGAAGCCT 
TTTCTACAAC AGTTCTATTC ACAGACAGTT TCAACAGCCA GTGAACTACG TAAACCTATA 
TACTGGATTG TAGCTGCTAA AGCAATTGAT TATGAACAGA TGTTACTTCT GATGGCAAAT 
GTGAAGTGGG ATGTGAAAGA AATCATGTCA CAACACAATA TTTATGTCGA TTCACTCTTA 
AAAGAATTTG AACAGTTTAA CAAGAGGATG AATGAAGTGT CAAAGAGAGT TCGTATTCCA 
TTGCCAGTTT CAAATATTCT TTGGGAACAC TGTATACGGT TAGCCAATAG AACAATTGTA 
GAAGGTTATG CAAATGTCAA GAAGTGTAGC AATGAAGGTC GTGCTCTGAT GCAATTGGAT 
TTTCAACAGT TTTTAATGAA GCTGGAGAAA CTCACAGATA TTAGGCCTAT TCCAGACAAG 
GAATTTGTGG AGACCTATAT TAAAGCTTAC TATTTGACTG AAAATGACAT GGAACGTTGG 
ATCAAGGAAC ACAGAGAATA TTCCACCAAG CAACTGACCA ATTTGGTGAA TGTTTGTCTG 
GGAACGCACA TCAACAAAAA AGCTAAGCAG AAACTATTAG CAGCTATTGA TGACCTAGAT 
AGGCCTAAAA GATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015667057.1
CDS	101..2995
Translation	MQKIKSLMTRQGLKSPQESIHDLSPLENFRIPSKEELRELREQPIDPQAQQEIINNIEEIYFSNDSFDIVKYELEKLPTVLSLQELEEYRDKLKQQQAAVSKKVADLILEKQPAYVKELERVTSLQTSLQLAAVICTNGRRHLNTAKEGFTQASLGLLANQRKRQLLVGLLKSLRTIKTLQQTDVRLSEMLEEEDYPGAIQLCLECQKAASTFKHYSCISELNSKLQDTLEQIEEQLDVALSKICKNFDINHYTKVQQAYRLLGKTQTAMDQLHMHFTQAIHNTVFQVVLGYVELCAGNTDTKFQKLQYKDLCTHITSDSYIPCLADLCKALWEVMLSYYRTMDWHEKYDHGESPSSSDGNNILDTEETNFDRSYVKKKLEHGLSRIWQDVQLKVKTYLLGTDMSNFKYDDFIFVLDIISRLVQVGEEFCGSKSEVLQDSIRKQSVNYFKNYHRTRLEELRMFLENETWELCPVKSSFSILQLHEFKFMAQSRSPTVSPSKQPTLETEGDLTLFEQYRNGGNPFEIQANNKEEETEDVLASNGYESDEQEKSAYQEYDSDSDVPEELKRDYVDEQTGDAPVKSVSRETLRTRKRSDYCLNKVNAPILTNTTLNVIRLVGKYMQMMNILKPIAFDVIHFMSQLFDYYMYAIYTFFGRNNTFESTGLGLSSSRLRTTLNRIQESLIDLDVSSDAAGTLTAVEERKEKVPMPHLSHLVILTSSNSLYGLAERVVATESLVFLAEQFEFLQSHLDAVMPPAKKPFLQQFYSQTVSTASELRKPIYWIVAAKAIDYEQMLLLMANVKWDVKEIMSQHNIYVDSLLKEFEQFNKRMNEVSKRVRIPLPVSNILWEHCIRLANRTIVEGYANVKKCSNEGRALMQLDFQQFLMKLEKLTDIRPIPDKEFVETYIKAYYLTENDMERWIKEHREYSTKQLTNLVNVCLGTHINKKAKQKLLAAIDDLDRPKR

Target ORF information:

RefSeq Version	XM_015811571.1
Organism	Protobothrops mucrosquamatus
Definition	Protobothrops mucrosquamatus VPS50 subunit of EARP/GARPII complex (VPS50), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015811571.1

ORF Insert Sequence:

ATGCAGAAAA TCAAGTCTCT GATGACCCGA CAGGGTCTTA AAAGTCCTCA GGAGAGTATT 
CATGATTTGA GCCCCCTTGA GAACTTCAGA ATTCCAAGCA AGGAAGAACT TAGAGAACTC 
CGGGAGCAAC CAATTGATCC TCAAGCACAG CAGGAAATAA TTAACAACAT TGAAGAAATC 
TATTTTTCCA ATGATTCCTT TGATATTGTT AAGTATGAGT TAGAGAAGCT GCCAACTGTT 
CTTAGTCTTC AGGAACTGGA AGAGTATAGA GACAAATTAA AGCAACAGCA AGCAGCTGTA 
TCCAAAAAAG TTGCAGACTT GATCCTTGAG AAACAGCCTG CATATGTCAA GGAACTTGAA 
CGAGTTACAT CATTACAAAC AAGCCTTCAG TTAGCAGCTG TTATCTGCAC AAATGGAAGA 
CGGCATTTGA ATACAGCCAA GGAGGGCTTT ACTCAAGCCA GCTTGGGTCT TCTTGCAAAT 
CAACGGAAAC GCCAGTTACT GGTTGGGCTG CTAAAATCTC TAAGAACAAT AAAAACCCTG 
CAACAGACTG ATGTGCGTTT GAGTGAAATG TTGGAGGAGG AAGATTATCC AGGGGCTATA 
CAATTGTGCC TGGAGTGCCA GAAGGCAGCT AGCACTTTCA AACACTACAG TTGCATAAGT 
GAATTGAATT CAAAATTACA GGACACCTTA GAGCAAATTG AGGAGCAGTT AGATGTTGCA 
CTTTCTAAAA TATGCAAGAA CTTTGATATA AACCATTATA CTAAGGTGCA GCAGGCATAC 
AGGCTACTTG GAAAAACACA GACAGCAATG GACCAACTGC ATATGCACTT TACTCAAGCC 
ATTCACAATA CAGTTTTCCA GGTTGTCCTT GGATATGTTG AATTGTGTGC AGGAAACACC 
GACACCAAAT TTCAGAAGCT TCAGTATAAG GATCTTTGCA CACACATTAC ATCAGACAGT 
TACATTCCAT GCCTTGCTGA TCTGTGCAAA GCTCTATGGG AGGTCATGCT GAGTTATTAT 
CGGACAATGG ACTGGCATGA AAAATATGAC CATGGGGAAT CTCCTTCATC TTCTGATGGA 
AATAATATCC TGGATACTGA GGAGACCAAT TTTGATCGTA GCTATGTGAA AAAAAAATTG 
GAGCATGGGC TTTCACGTAT ATGGCAGGAT GTCCAATTGA AAGTGAAGAC CTATTTGCTA 
GGCACCGATA TGTCTAACTT CAAATATGAT GACTTCATAT TTGTACTGGA TATTATCAGC 
AGACTGGTGC AGGTTGGCGA AGAATTTTGT GGCAGCAAGT CAGAAGTCTT GCAAGATTCC 
ATTAGAAAAC AAAGTGTTAA TTATTTCAAG AATTATCACA GAACTCGTCT TGAAGAACTG 
CGAATGTTCT TAGAGAATGA GACTTGGGAA CTTTGTCCTG TTAAATCAAG TTTCAGCATA 
TTGCAACTTC ATGAATTTAA GTTTATGGCA CAATCACGTT CCCCTACTGT GTCACCGAGC 
AAGCAGCCTA CGTTAGAAAC AGAAGGAGAT CTAACTCTTT TTGAGCAGTA TCGTAATGGA 
GGAAATCCCT TTGAAATCCA AGCCAACAAC AAGGAGGAAG AGACAGAAGA TGTGTTGGCT 
TCCAATGGGT ATGAATCGGA TGAACAAGAA AAGAGCGCCT ATCAAGAGTA TGATAGTGAC 
AGTGATGTTC CTGAAGAACT GAAAAGGGAC TATGTTGATG AACAGACTGG AGATGCCCCA 
GTAAAAAGTG TTTCTCGAGA AACTCTGAGG ACCAGGAAGA GATCAGATTA TTGCCTCAAC 
AAGGTGAATG CCCCGATTTT AACCAACACT ACACTTAATG TAATTAGGCT TGTTGGTAAA 
TACATGCAAA TGATGAACAT TTTGAAGCCA ATTGCATTTG ATGTGATTCA CTTTATGTCG 
CAGCTTTTTG ATTACTACAT GTATGCAATA TATACATTTT TTGGCCGAAA TAACACATTT 
GAATCAACAG GACTTGGACT TAGCAGCAGT AGGCTTCGTA CTACCTTGAA TAGAATCCAG 
GAAAGCTTGA TCGATTTGGA TGTCTCCTCT GATGCTGCTG GAACACTAAC TGCTGTTGAA 
GAAAGAAAGG AAAAGGTTCC AATGCCACAT CTAAGTCATC TTGTAATCTT GACATCCAGC 
AACAGTCTCT ATGGCTTGGC AGAGAGAGTG GTAGCCACAG AATCCTTAGT ATTTTTGGCT 
GAACAATTTG AGTTCCTCCA GTCCCATCTT GATGCTGTAA TGCCTCCAGC CAAGAAGCCT 
TTTCTACAAC AGTTCTATTC ACAGACAGTT TCAACAGCCA GTGAACTACG TAAACCTATA 
TACTGGATTG TAGCTGCTAA AGCAATTGAT TATGAACAGA TGTTACTTCT GATGGCAAAT 
GTGAAGTGGG ATGTGAAAGA AATCATGTCA CAACACAATA TTTATGTCGA TTCACTCTTA 
AAAGAATTTG AACAGTTTAA CAAGAGGATG AATGAAGTGT CAAAGAGAGT TCGTATTCCA 
TTGCCAGTTT CAAATATTCT TTGGGAACAC TGTATACGGT TAGCCAATAG AACAATTGTA 
GAAGGTTATG CAAATGTCAA GAAGTGTAGC AATGAAGGTC GTGCTCTGAT GCAATTGGAT 
TTTCAACAGT TTTTAATGAA GCTGGAGAAA CTCACAGATA TTAGGCCTAT TCCAGACAAG 
GAATTTGTGG AGACCTATAT TAAAGCTTAC TATTTGACTG AAAATGACAT GGAACGTTGG 
ATCAAGGAAC ACAGAGAATA TTCCACCAAG CAACTGACCA ATTTGGTGAA TGTTTGTCTG 
GGAACGCACA TCAACAAAAA AGCTAAGCAG AAACTATTAG CAGCTATTGA TGACCTAGAT 
AGGCCTAAAA GATAA