EEFSEC cDNA ORF clone, Propithecus coquereli(Coquerel's sifaka)

Gene
Clones
gRNAs

Summary

Gene Symbol	EEFSEC
Entrez Gene ID	105822037
Full Name	eukaryotic elongation factor, selenocysteine-tRNA specific
Gene Type	protein-coding
Organism	Propithecus coquereli(Coquerel's sifaka)

mRNA and Protein(s)

mRNA	Protein	Name
XM_012658965.1	XP_012514419.1	selenocysteine-specific elongation factor

Pathways from BioSystems
Gene Ontology

The following EEFSEC gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the EEFSEC cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OPr115527	XM_012658965.1 Latest version!	Propithecus coquereli eukaryotic elongation factor, selenocysteine-tRNA-specific (EEFSEC), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$342.30 $489.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

EEFSEC ( XM_012658965.1 ) cDNA ORF clone, Propithecus coquereli(Coquerel's sifaka) -> XP_012514419.1 Propithecus coquereli eukaryotic elongation factor, selenocysteine-tRNA-specific (EEFSEC), mRNA.

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CloneID	OPr115527
Clone ID Related Accession (Same CDS sequence)	XM_012658965.1
Accession Version	XM_012658965.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1779bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-05-31
Organism	Propithecus coquereli(Coquerel's sifaka)
Product	selenocysteine-specific elongation factor
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_012151863.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Propithecus coquereli Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGGGC GGCGGGTGAA CGTGAACGTG GGCGTGCTGG GCCACATCGA CAGCGGCAAG 
ACGGCGCTGG CGCGCGCGCT GAGCACCACG GCCTCCACCG CCGCCTTCGA CAAGCAGCCG 
CAGAGCCGGG AGCGCGGCAT CACGCTCGAC CTGGGCTTCT CCTGCTTCTG CGTGCCGCTG 
CCCGCGCGCC TGCGGTCGGC CCTGCCCGCG CCCGACGCGG CGGCCGAGCC CGGGGAGCCG 
CTGCTGCAGG TCACGCTGGT CGACTGTCCC GGGCACGCCT CCCTCATCCG GACCATCATC 
GGCGGCGCCC AGATCATTGA TCTGATGCTG TTGGTCATCG ATGTCACCAA GGGCATGCAG 
ACCCAGTCGG CGGAATGCCT GGTCATCGGC CAGATCGCCT GCCAGAGGCT GGTCGTCGTG 
CTCAACAAAA CGGACCTCCT GGCCGAAGAC AAGAGACAGG CGGCCATTGA GAAGATGACC 
AGGAAAATGC GCAAGACGCT GGAGAACACC AAGTTCCGAG GCGCACCGAT CGTGCCCGTG 
GCAGCCAAGC CCGGGGGGCC CGACGCCCCC GAATCCGAAG CTCCGCAGGG CATTTCGGAG 
CTCATCGAGC TCCTGACGTC CCAGATCTCC GTCCCGGCGA GGGACCCCTC GGGCCCGTTC 
CTCATGTCCG TGGACCACTG TTTCTCCATC AGAGGCCAAG GCACCGTGAT GACGGGGACC 
ATCCTGTCGG GCTCCATCAG CCTCGGCGAC AGCGTGGAGA TTCCCGCCCT CAAGGTGGTG 
AAGAAGGTCA AGTCCATGCA GTCCTTCCAC ACGCCGGTCA CCGCCGCCAC GCAGGGGGAC 
CGGCTGGGCA TCTGCGTGAC CCAGTTCGAC CCCAAGCTGC TGGAGCGCGG CCTCGCGTGC 
TCCCCGGGGA CCCTGCACAC CGTCCACGCC GCCCTCGTCT CTGTGGAGAA GATCCCGTAC 
TTCCGGGGTC CCCTGCAGAC CAAGGCCAAG TTCCACGTCA CCGTGGGCCA CGACACGGTC 
ATGGGCCGGG TCATGTTCTT CGGGCCGGCC CCCGACGGTG CGGACGGCGA GGCCGCGCCG 
GACGCCTTCG ACTTCTCCCG GGAATACCTG TTCCAGGAGC AGTACCTGTG CAAGGACCCG 
GCGCCCGCGG GGACGGGCGG GGACACGGGG GACACGAAGG CGGGCCCGGC CGCGGAGGGC 
GGCTGTCCCC GGCAGCAGTG GGCGCTGCTG GAGTTCGAGA GGCCCGTGAC CTGCCCGCGG 
CGCTGCCTGG TCATCGGCTC GAGGCTGGAC GCGGACGTCC ACGCCAGCGC CTGTCGCCTG 
GCCTTCCACG GCGTCCTGCT GCACGGGCTG GAGGACAGGG GCTACGCCGA GACCTGCCTG 
CCGTCCCTGA AGGTGTTCAA GATGAAGCAC CGGCACGGCG TCGTGGAGCG GGCGATGGAC 
GACCACAGCG TGATCGGCCG CTCGCTGTTC AAGAAGGAGA CCAACATCCA GCTGTTCGTG 
GGGCTCCGAG TGCGACTGTC CACCGGCGAG CTGGGCGTCA TCGACAGCGC CTTCGGCCAG 
AGCGGCAAGT TCAAAGTCCT CGTCCCCGGT GGCCTCAGCC CCGAGTCCAG GAGGATCCTG 
ACGCCCGCCC TCAAGAAACG GGGCCGCGGG GGCCGCGGGG AGGCGGCCGG GCCGGAGGAG 
GGTGCCGAGC GCGCGGAGGC CACCCAGCAC GTGACACTCA GCCTGTCTTT CAAGCGTTAC 
GTCTTTGACC CCCACAAGCG CATGGTCCAG TCCCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012514419.1
CDS	27..1805
Translation	MAGRRVNVNVGVLGHIDSGKTALARALSTTASTAAFDKQPQSRERGITLDLGFSCFCVPLPARLRSALPAPDAAAEPGEPLLQVTLVDCPGHASLIRTIIGGAQIIDLMLLVIDVTKGMQTQSAECLVIGQIACQRLVVVLNKTDLLAEDKRQAAIEKMTRKMRKTLENTKFRGAPIVPVAAKPGGPDAPESEAPQGISELIELLTSQISVPARDPSGPFLMSVDHCFSIRGQGTVMTGTILSGSISLGDSVEIPALKVVKKVKSMQSFHTPVTAATQGDRLGICVTQFDPKLLERGLACSPGTLHTVHAALVSVEKIPYFRGPLQTKAKFHVTVGHDTVMGRVMFFGPAPDGADGEAAPDAFDFSREYLFQEQYLCKDPAPAGTGGDTGDTKAGPAAEGGCPRQQWALLEFERPVTCPRRCLVIGSRLDADVHASACRLAFHGVLLHGLEDRGYAETCLPSLKVFKMKHRHGVVERAMDDHSVIGRSLFKKETNIQLFVGLRVRLSTGELGVIDSAFGQSGKFKVLVPGGLSPESRRILTPALKKRGRGGRGEAAGPEEGAERAEATQHVTLSLSFKRYVFDPHKRMVQSP

Target ORF information:

RefSeq Version	XM_012658965.1
Organism	Propithecus coquereli(Coquerel's sifaka)
Definition	Propithecus coquereli eukaryotic elongation factor, selenocysteine-tRNA-specific (EEFSEC), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012658965.1

ORF Insert Sequence:

ATGGCGGGGC GGCGGGTGAA CGTGAACGTG GGCGTGCTGG GCCACATCGA CAGCGGCAAG 
ACGGCGCTGG CGCGCGCGCT GAGCACCACG GCCTCCACCG CCGCCTTCGA CAAGCAGCCG 
CAGAGCCGGG AGCGCGGCAT CACGCTCGAC CTGGGCTTCT CCTGCTTCTG CGTGCCGCTG 
CCCGCGCGCC TGCGGTCGGC CCTGCCCGCG CCCGACGCGG CGGCCGAGCC CGGGGAGCCG 
CTGCTGCAGG TCACGCTGGT CGACTGTCCC GGGCACGCCT CCCTCATCCG GACCATCATC 
GGCGGCGCCC AGATCATTGA TCTGATGCTG TTGGTCATCG ATGTCACCAA GGGCATGCAG 
ACCCAGTCGG CGGAATGCCT GGTCATCGGC CAGATCGCCT GCCAGAGGCT GGTCGTCGTG 
CTCAACAAAA CGGACCTCCT GGCCGAAGAC AAGAGACAGG CGGCCATTGA GAAGATGACC 
AGGAAAATGC GCAAGACGCT GGAGAACACC AAGTTCCGAG GCGCACCGAT CGTGCCCGTG 
GCAGCCAAGC CCGGGGGGCC CGACGCCCCC GAATCCGAAG CTCCGCAGGG CATTTCGGAG 
CTCATCGAGC TCCTGACGTC CCAGATCTCC GTCCCGGCGA GGGACCCCTC GGGCCCGTTC 
CTCATGTCCG TGGACCACTG TTTCTCCATC AGAGGCCAAG GCACCGTGAT GACGGGGACC 
ATCCTGTCGG GCTCCATCAG CCTCGGCGAC AGCGTGGAGA TTCCCGCCCT CAAGGTGGTG 
AAGAAGGTCA AGTCCATGCA GTCCTTCCAC ACGCCGGTCA CCGCCGCCAC GCAGGGGGAC 
CGGCTGGGCA TCTGCGTGAC CCAGTTCGAC CCCAAGCTGC TGGAGCGCGG CCTCGCGTGC 
TCCCCGGGGA CCCTGCACAC CGTCCACGCC GCCCTCGTCT CTGTGGAGAA GATCCCGTAC 
TTCCGGGGTC CCCTGCAGAC CAAGGCCAAG TTCCACGTCA CCGTGGGCCA CGACACGGTC 
ATGGGCCGGG TCATGTTCTT CGGGCCGGCC CCCGACGGTG CGGACGGCGA GGCCGCGCCG 
GACGCCTTCG ACTTCTCCCG GGAATACCTG TTCCAGGAGC AGTACCTGTG CAAGGACCCG 
GCGCCCGCGG GGACGGGCGG GGACACGGGG GACACGAAGG CGGGCCCGGC CGCGGAGGGC 
GGCTGTCCCC GGCAGCAGTG GGCGCTGCTG GAGTTCGAGA GGCCCGTGAC CTGCCCGCGG 
CGCTGCCTGG TCATCGGCTC GAGGCTGGAC GCGGACGTCC ACGCCAGCGC CTGTCGCCTG 
GCCTTCCACG GCGTCCTGCT GCACGGGCTG GAGGACAGGG GCTACGCCGA GACCTGCCTG 
CCGTCCCTGA AGGTGTTCAA GATGAAGCAC CGGCACGGCG TCGTGGAGCG GGCGATGGAC 
GACCACAGCG TGATCGGCCG CTCGCTGTTC AAGAAGGAGA CCAACATCCA GCTGTTCGTG 
GGGCTCCGAG TGCGACTGTC CACCGGCGAG CTGGGCGTCA TCGACAGCGC CTTCGGCCAG 
AGCGGCAAGT TCAAAGTCCT CGTCCCCGGT GGCCTCAGCC CCGAGTCCAG GAGGATCCTG 
ACGCCCGCCC TCAAGAAACG GGGCCGCGGG GGCCGCGGGG AGGCGGCCGG GCCGGAGGAG 
GGTGCCGAGC GCGCGGAGGC CACCCAGCAC GTGACACTCA GCCTGTCTTT CAAGCGTTAC 
GTCTTTGACC CCCACAAGCG CATGGTCCAG TCCCCCTGA