POPTR_0006s27430g cDNA ORF clone, Populus trichocarpa(Populus balsamifera subsp. trichocarpa)

Gene
Clones
gRNAs

Summary

Gene Symbol	POPTR_0006s27430g
Entrez Gene ID	7489126
Full Name	WD-40 repeat family protein
Synonyms	POPTRDRAFT_716902
Gene Type	protein-coding
Organism	Populus trichocarpa(Populus balsamifera subsp. trichocarpa)

mRNA and Protein(s)

mRNA	Protein	Name
XM_024603956.1	XP_024459724.1	topless-related protein 1 isoform X2
XM_024603955.1	XP_024459723.1	topless-related protein 1 isoform X1
XM_024603957.1	XP_024459725.1	topless-related protein 1 isoform X3
XM_002308645.2	XP_002308681.1	WD-40 repeat family protein

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

The genome of black cottonwood, Populus trichocarpa (Torr. & Gray).
Tuskan GA, Difazio S, Jansson S, Bohlmann J, Grigoriev I, Hellsten U, Putnam N, Ralph S, Rombauts S, Salamov A, Schein J, Sterck L, Aerts A, Bhalerao RR, Bhalerao RP, Blaudez D, Boerjan W, Brun A, Brunner A, Busov V, Campbell M, Carlson J, Chalot M, Chapman J, Chen GL, Cooper D, Coutinho PM, Couturier J, Covert S, Cronk Q, Cunningham R, Davis J, Degroeve S, D?jardin A, Depamphilis C, Detter J, Dirks B, Dubchak I, Duplessis S, Ehlting J, Ellis B, Gendler K, Goodstein D, Gribskov M, Grimwood J, Groover A, Gunter L, Hamberger B, Heinze B, Helariutta Y, Henrissat B, Holligan D, Holt R, Huang W, Islam-Faridi N, Jones S, Jones-Rhoades M, Jorgensen R, Joshi C, Kangasj?rvi J, Karlsson J, Kelleher C, Kirkpatrick R, Kirst M, Kohler A, Kalluri U, Larimer F, Leebens-Mack J, Lepl? JC, Locascio P, Lou Y, Lucas S, Martin F, Montanini B, Napoli C, Nelson DR, Nelson C, Nieminen K, Nilsson O, Pereda V, Peter G, Philippe R, Pilate G, Poliakov A, Razumovskaya J, Richardson P, Rinaldi C, Ritland K, Rouz? P, Ryaboy D, Schmutz J, Schrader J, Segerman B, Shin H, Siddiqui A, Sterky F, Terry A, Tsai CJ, Uberbacher E, Unneberg P, Vahala J, Wall K, Wessler S, Yang G, Yin T, Douglas C, Marra M, Sandberg G, Van de Peer Y, Rokhsar D
Science (New York, N.Y.)313(5793)1596-604(2006 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following POPTR_0006s27430g gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the POPTR_0006s27430g cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OPa432166	XM_024603957.1 Latest version!	Populus trichocarpa topless-related protein 1 (LOC7489126), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OPa432164	XM_024603955.1 Latest version!	Populus trichocarpa topless-related protein 1 (LOC7489126), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OPa432165	XM_024603956.1 Latest version!	Populus trichocarpa topless-related protein 1 (LOC7489126), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OPa15431	XM_002308645.2 Latest version!	Populus trichocarpa WD-40 repeat family protein (POPTR_0006s27430g) mRNA, complete cds.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

POPTR_0006s27430g ( XM_024603957.1 ) cDNA ORF clone, Populus trichocarpa(Populus balsamifera subsp. trichocarpa) -> XP_024459725.1 Populus trichocarpa topless-related protein 1 (LOC7489126), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OPa432166
Clone ID Related Accession (Same CDS sequence)	XM_024603957.1
Accession Version	XM_024603957.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2931bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-04-08
Organism	Populus trichocarpa(black cottonwood)
Product	topless-related protein 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037290.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Populus trichocarpa Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTTTGC TTTTGACATT GAATGATATA AGGGACCATG AGTCACTCTC CATGTATAGT 
GATGCAGACT CTGCAAGAAA AGTTATGAGG GTTGAGCTTA AGAAACTTAT TGAAGCAAAC 
CCCCTTTTCA GCGACAAATT GGAATTTCCG AATGCTGCAA GTCATAGATT ACGACGCCTC 
ATAAACCAAA GTCTGAATTG GCAGCATGTT CTTTGTGCAT ATCCACAGCC AAATCCTGAT 
ATAAGAACCC TTTTTGTGGA TCATGTCTGT GTACCAATTC CATCAGATGA TCATTTATTT 
TCCACATTGA GTGATAGCAA TCCAGTGCCT TCTCAAACCA CATCAATGCT AGTTTCAACT 
TCCTCAGCCT CAAATTCTAC CTCATCTTCT GAGGCTCACT CTTCAATTTC AAGTGAAGCT 
TTGTCTCTCG GTGATCCAAC AAACATAGGA ATTGCTACTA TTACAGATGG TTCGGAGGAT 
ATTACTAGTG TACATTACAG TAGTATTCCT GAAAGTGAAC TTGCAATCCT AAAAAGGCCT 
TCTGACGAGG AAATATCAGC TGACTCTCAT CCTGATCAAA GCTCCATCGA CATTTCTGAT 
GACTTGCCTA AGAATGTTTT AAGGATATTA AATGAGGGGT CATCTCCAAC AAGCATGGAT 
TTTCACCCTG AACATCAGAC TGTTCTTCTA GTTGGAACTT CTGTGGGGGA CATAGGATTG 
TGGGAAGTTA GATCAGGGGA AAGTTTACTT TCAAGGAATT TTAAGGTCTG GGATATTGCA 
GCATGCTCAA AGATTTTCAA GGCAACTTTG CTCAAGGATC CTAGTGTTTC TGTGAATCGT 
GTTGCTTGGA GCCCAGAAGG TTGTTTCTTC GGGGTTGCGT ATTCAAAACA CATAGTGCAA 
ATATATTCTT ATAATGATGC CAAAGATGTA CAGCAGAAAT TGGAGATTGA TGCTCATGTT 
GGTGGTGTAA ATGACCTAGC ATTCTCTGCC CCTGAGAAAC AACTGTTGGT CATTACATGT 
GGTGATGACA AGACAGTTAA GGCATGGGAT GTGACCAGTG GTGTCAAAAT GTATTCTTTT 
GAAGGCCATG ATGCTCCTGT TTATTCTCTC TGTCCTCATA GCAAGGGAAA CGTTCATTTT 
CTCTCTGCAA CATCAGTGAA CAGCAACATA AAAGTGTGCC TATATGATAA TCTAGGAGCT 
AAAGTAGATT ATGATGCTCC TGGCCTGGGG TGTACATCAA TGGCTTATAG TGGTGATAGA 
AGGCTTTTTT CATGTGGGAC CAGTAAATCT GGAGAGTCAT TTCTTGCTGA ATGGGGTGAC 
TCTGAAGGTT CCATAAAAAG AACCTATCTA GGATTGCAGA AGAGTTCTTC GGGTGTAGTG 
CAGTTTGATA TAATGAAGAA CCAGGTTTTA GCTGCTGGTG ATGAGCATGT GATCAAATTA 
TGGGATATGG ACAAAGTTGA GCTTTTCACA ACCATTGATG CTGAGGGAGG CTTACCAGAA 
AACCCTCGTG TTCGATTCAA TAAGGAAGGC ACGCTGTTAG CTGTTTCTGC AAATGACAAC 
AAAATCAAGA TCTTGGCAAA AGATAGCGGT CTCCATTCAC TGCATACATC ACAAAATTGT 
TCTGATGATG CTTCTAGAGA TCTCTGTCAT AACTTTAAGA AGCTTGGTAT TGAACCGAGT 
TCAACTGTTG CTTGTGCTGG AGCTGCAGAT GAAGCTGTCA CAAACAATGG AAACCCAGAA 
AACTCGGAGG TAGTGAAATC TAAAATTACT GGAAAATCCA CTACATCAAA GAGCGGGAGG 
CTCATTTTAA TAACAAGCCC TTCTCAGTTC CAGATTCTGC GACTTCCTTC TCCCATGAAA 
GCAAACAAGA TATCGAGGCT CATTTACAAC AATGCGGGTA ACTCCATTTT GGCATTAACA 
TCAAATGCCT CCCATTTATG TTGGAAATGG TCACAGAATG ACACTCACTC GAGTGACAAG 
GCAACTGCAA AAGTTCCCCC TCAACTGTGG CAACCCAGTA GTAGCTCAGG ACTGATGACA 
AATGACCTTA CAGGCAGCAG CCCTGAAGAG GCAGTTCCCT GCTTTGCCCT GTCTAAGAAT 
GATTCATATC TCCTGTCAGC ATGTGGAGGG AGAATTTCTT TGTATAGCTT GCTGAAATTC 
AAGACAATGC TGCCTATCAT GCAACCACCT GCAGCAACAT GTATTGCTTT CTACCCTCAA 
GATAACAACA TACTTGCTAT AGGGAGAGAT GATTCCACGA TTCTAATTTA TAATGTCCGT 
TCAGCTAAGG TCGATACCAT ACTTGAGGGT CATTCTAAAA GAGTCTCTGG TCTTGCATTC 
TCTAATGATC TGAATGTACT TGTTTCTTCT GGAGCAGATG CTCAGATTTT TGTCTGGAAA 
GTTGAAGGGT GGGGAAAAGA GAGGAGCAGA TTCTTGCAGA TTCCCGATGA CAGAACACTT 
TCTTCATTAT CATTAGATAC AGACATCCAG TTTCATCAGA ATCAGACAGA ATTCCTTGCA 
GTACATGAGA CTTGCCTTTC AATCTATGAC GCCAGAAAAT TAGAGTGTGT AAAGCAGTGG 
AGTCCAGGCG ATTTTGGAGC ACCAATCTCC CATGCAACAT TCTCGTGTGA TGGCCAGATG 
GTGTATGCTA GCTTTGAAGA TGGGCTTGTA TCTATATTTG ATGCTTCGGA TTTTCAGTTA 
TACTGTCGGA TTAATCCAAC TGCTTACCTT TCTCCCACTT CAAGTTTGGG AGTGTATCCG 
CTTGTCGTTG CCGCACATCC TCAGGAACCT GACCAGTTTG CAGTGGGACT GAAAGATGGT 
GCTGTTATTG TCTTTGAGCC TCCAATTTCT GCCGGTAAAT GGAGTATGCT GACAGCATAT 
GAAAACGGAT CAGCAAGTAA AATACCTGCT GAATCCGAAG CTAATCAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024459725.1
CDS	480..3410
Translation	MTLLLTLNDIRDHESLSMYSDADSARKVMRVELKKLIEANPLFSDKLEFPNAASHRLRRLINQSLNWQHVLCAYPQPNPDIRTLFVDHVCVPIPSDDHLFSTLSDSNPVPSQTTSMLVSTSSASNSTSSSEAHSSISSEALSLGDPTNIGIATITDGSEDITSVHYSSIPESELAILKRPSDEEISADSHPDQSSIDISDDLPKNVLRILNEGSSPTSMDFHPEHQTVLLVGTSVGDIGLWEVRSGESLLSRNFKVWDIAACSKIFKATLLKDPSVSVNRVAWSPEGCFFGVAYSKHIVQIYSYNDAKDVQQKLEIDAHVGGVNDLAFSAPEKQLLVITCGDDKTVKAWDVTSGVKMYSFEGHDAPVYSLCPHSKGNVHFLSATSVNSNIKVCLYDNLGAKVDYDAPGLGCTSMAYSGDRRLFSCGTSKSGESFLAEWGDSEGSIKRTYLGLQKSSSGVVQFDIMKNQVLAAGDEHVIKLWDMDKVELFTTIDAEGGLPENPRVRFNKEGTLLAVSANDNKIKILAKDSGLHSLHTSQNCSDDASRDLCHNFKKLGIEPSSTVACAGAADEAVTNNGNPENSEVVKSKITGKSTTSKSGRLILITSPSQFQILRLPSPMKANKISRLIYNNAGNSILALTSNASHLCWKWSQNDTHSSDKATAKVPPQLWQPSSSSGLMTNDLTGSSPEEAVPCFALSKNDSYLLSACGGRISLYSLLKFKTMLPIMQPPAATCIAFYPQDNNILAIGRDDSTILIYNVRSAKVDTILEGHSKRVSGLAFSNDLNVLVSSGADAQIFVWKVEGWGKERSRFLQIPDDRTLSSLSLDTDIQFHQNQTEFLAVHETCLSIYDARKLECVKQWSPGDFGAPISHATFSCDGQMVYASFEDGLVSIFDASDFQLYCRINPTAYLSPTSSLGVYPLVVAAHPQEPDQFAVGLKDGAVIVFEPPISAGKWSMLTAYENGSASKIPAESEANQ

Target ORF information:

RefSeq Version	XM_024603957.1
Organism	Populus trichocarpa(black cottonwood)
Definition	Populus trichocarpa topless-related protein 1 (LOC7489126), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024603957.1

ORF Insert Sequence:

ATGACTTTGC TTTTGACATT GAATGATATA AGGGACCATG AGTCACTCTC CATGTATAGT 
GATGCAGACT CTGCAAGAAA AGTTATGAGG GTTGAGCTTA AGAAACTTAT TGAAGCAAAC 
CCCCTTTTCA GCGACAAATT GGAATTTCCG AATGCTGCAA GTCATAGATT ACGACGCCTC 
ATAAACCAAA GTCTGAATTG GCAGCATGTT CTTTGTGCAT ATCCACAGCC AAATCCTGAT 
ATAAGAACCC TTTTTGTGGA TCATGTCTGT GTACCAATTC CATCAGATGA TCATTTATTT 
TCCACATTGA GTGATAGCAA TCCAGTGCCT TCTCAAACCA CATCAATGCT AGTTTCAACT 
TCCTCAGCCT CAAATTCTAC CTCATCTTCT GAGGCTCACT CTTCAATTTC AAGTGAAGCT 
TTGTCTCTCG GTGATCCAAC AAACATAGGA ATTGCTACTA TTACAGATGG TTCGGAGGAT 
ATTACTAGTG TACATTACAG TAGTATTCCT GAAAGTGAAC TTGCAATCCT AAAAAGGCCT 
TCTGACGAGG AAATATCAGC TGACTCTCAT CCTGATCAAA GCTCCATCGA CATTTCTGAT 
GACTTGCCTA AGAATGTTTT AAGGATATTA AATGAGGGGT CATCTCCAAC AAGCATGGAT 
TTTCACCCTG AACATCAGAC TGTTCTTCTA GTTGGAACTT CTGTGGGGGA CATAGGATTG 
TGGGAAGTTA GATCAGGGGA AAGTTTACTT TCAAGGAATT TTAAGGTCTG GGATATTGCA 
GCATGCTCAA AGATTTTCAA GGCAACTTTG CTCAAGGATC CTAGTGTTTC TGTGAATCGT 
GTTGCTTGGA GCCCAGAAGG TTGTTTCTTC GGGGTTGCGT ATTCAAAACA CATAGTGCAA 
ATATATTCTT ATAATGATGC CAAAGATGTA CAGCAGAAAT TGGAGATTGA TGCTCATGTT 
GGTGGTGTAA ATGACCTAGC ATTCTCTGCC CCTGAGAAAC AACTGTTGGT CATTACATGT 
GGTGATGACA AGACAGTTAA GGCATGGGAT GTGACCAGTG GTGTCAAAAT GTATTCTTTT 
GAAGGCCATG ATGCTCCTGT TTATTCTCTC TGTCCTCATA GCAAGGGAAA CGTTCATTTT 
CTCTCTGCAA CATCAGTGAA CAGCAACATA AAAGTGTGCC TATATGATAA TCTAGGAGCT 
AAAGTAGATT ATGATGCTCC TGGCCTGGGG TGTACATCAA TGGCTTATAG TGGTGATAGA 
AGGCTTTTTT CATGTGGGAC CAGTAAATCT GGAGAGTCAT TTCTTGCTGA ATGGGGTGAC 
TCTGAAGGTT CCATAAAAAG AACCTATCTA GGATTGCAGA AGAGTTCTTC GGGTGTAGTG 
CAGTTTGATA TAATGAAGAA CCAGGTTTTA GCTGCTGGTG ATGAGCATGT GATCAAATTA 
TGGGATATGG ACAAAGTTGA GCTTTTCACA ACCATTGATG CTGAGGGAGG CTTACCAGAA 
AACCCTCGTG TTCGATTCAA TAAGGAAGGC ACGCTGTTAG CTGTTTCTGC AAATGACAAC 
AAAATCAAGA TCTTGGCAAA AGATAGCGGT CTCCATTCAC TGCATACATC ACAAAATTGT 
TCTGATGATG CTTCTAGAGA TCTCTGTCAT AACTTTAAGA AGCTTGGTAT TGAACCGAGT 
TCAACTGTTG CTTGTGCTGG AGCTGCAGAT GAAGCTGTCA CAAACAATGG AAACCCAGAA 
AACTCGGAGG TAGTGAAATC TAAAATTACT GGAAAATCCA CTACATCAAA GAGCGGGAGG 
CTCATTTTAA TAACAAGCCC TTCTCAGTTC CAGATTCTGC GACTTCCTTC TCCCATGAAA 
GCAAACAAGA TATCGAGGCT CATTTACAAC AATGCGGGTA ACTCCATTTT GGCATTAACA 
TCAAATGCCT CCCATTTATG TTGGAAATGG TCACAGAATG ACACTCACTC GAGTGACAAG 
GCAACTGCAA AAGTTCCCCC TCAACTGTGG CAACCCAGTA GTAGCTCAGG ACTGATGACA 
AATGACCTTA CAGGCAGCAG CCCTGAAGAG GCAGTTCCCT GCTTTGCCCT GTCTAAGAAT 
GATTCATATC TCCTGTCAGC ATGTGGAGGG AGAATTTCTT TGTATAGCTT GCTGAAATTC 
AAGACAATGC TGCCTATCAT GCAACCACCT GCAGCAACAT GTATTGCTTT CTACCCTCAA 
GATAACAACA TACTTGCTAT AGGGAGAGAT GATTCCACGA TTCTAATTTA TAATGTCCGT 
TCAGCTAAGG TCGATACCAT ACTTGAGGGT CATTCTAAAA GAGTCTCTGG TCTTGCATTC 
TCTAATGATC TGAATGTACT TGTTTCTTCT GGAGCAGATG CTCAGATTTT TGTCTGGAAA 
GTTGAAGGGT GGGGAAAAGA GAGGAGCAGA TTCTTGCAGA TTCCCGATGA CAGAACACTT 
TCTTCATTAT CATTAGATAC AGACATCCAG TTTCATCAGA ATCAGACAGA ATTCCTTGCA 
GTACATGAGA CTTGCCTTTC AATCTATGAC GCCAGAAAAT TAGAGTGTGT AAAGCAGTGG 
AGTCCAGGCG ATTTTGGAGC ACCAATCTCC CATGCAACAT TCTCGTGTGA TGGCCAGATG 
GTGTATGCTA GCTTTGAAGA TGGGCTTGTA TCTATATTTG ATGCTTCGGA TTTTCAGTTA 
TACTGTCGGA TTAATCCAAC TGCTTACCTT TCTCCCACTT CAAGTTTGGG AGTGTATCCG 
CTTGTCGTTG CCGCACATCC TCAGGAACCT GACCAGTTTG CAGTGGGACT GAAAGATGGT 
GCTGTTATTG TCTTTGAGCC TCCAATTTCT GCCGGTAAAT GGAGTATGCT GACAGCATAT 
GAAAACGGAT CAGCAAGTAA AATACCTGCT GAATCCGAAG CTAATCAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

POPTR_0006s27430g ( XM_024603955.1 ) cDNA ORF clone, Populus trichocarpa(Populus balsamifera subsp. trichocarpa) -> XP_024459723.1 Populus trichocarpa topless-related protein 1 (LOC7489126), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OPa432164
Clone ID Related Accession (Same CDS sequence)	XM_024603955.1
Accession Version	XM_024603955.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3273bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-04-08
Organism	Populus trichocarpa(black cottonwood)
Product	topless-related protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037290.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Populus trichocarpa Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGCTC TCAGTAAAGA TCTTGTCTTG TTAATCTCAC AGTTCTTAGA TGAGGAAGGG 
TTCAAAGAAA CTGCTCGCAT GCTTGAGCGT GAAAGTAGTT ATTACTTCAA CATGAAGTTT 
TTTGAGGATA TGATATGCAG TGGTGATTGG GATGAGGCTG AGAGATATTT TTCTTGTTTC 
ACAAAATTAA CTGACAATAG ATTCTCGATG AAGGTCTATT TTGAAATTAG GAAGCAGAAA 
TTTCTGGAGG CATTGGACAA CAAGGACCGT GCCAAGGCTT TAGACATCCT TGTGAAGGAT 
TTGAAAACTT TTGTCTCATA TAATGAGGAG CTGTTTAAGG AAATGACTTT GCTTTTGACA 
TTGAATGATA TAAGGGACCA TGAGTCACTC TCCATGTATA GTGATGCAGA CTCTGCAAGA 
AAAGTTATGA GGGTTGAGCT TAAGAAACTT ATTGAAGCAA ACCCCCTTTT CAGCGACAAA 
TTGGAATTTC CGAATGCTGC AAGTCATAGA TTACGACGCC TCATAAACCA AAGTCTGAAT 
TGGCAGCATG TTCTTTGTGC ATATCCACAG CCAAATCCTG ATATAAGAAC CCTTTTTGTG 
GATCATGTCT GTGTACCAAT TCCATCAGAT GATCATTTAT TTTCCACATT GAGTGATAGC 
AATCCAGTGC CTTCTCAAAC CACATCAATG CTAGTTTCAA CTTCCTCAGC CTCAAATTCT 
ACCTCATCTT CTGAGGCTCA CTCTTCAATT TCAAGTGAAG CTTTGTCTCT CGGTGATCCA 
ACAAACATAG GAATTGCTAC TATTACAGAT GGTTCGGAGG ATATTACTAG TGTACATTAC 
AGTAGTATTC CTGAAAGTGA ACTTGCAATC CTAAAAAGGC CTTCTGACGA GGAAATATCA 
GCTGACTCTC ATCCTGATCA AAGCTCCATC GACATTTCTG ATGACTTGCC TAAGAATGTT 
TTAAGGATAT TAAATGAGGG GTCATCTCCA ACAAGCATGG ATTTTCACCC TGAACATCAG 
ACTGTTCTTC TAGTTGGAAC TTCTGTGGGG GACATAGGAT TGTGGGAAGT TAGATCAGGG 
GAAAGTTTAC TTTCAAGGAA TTTTAAGGTC TGGGATATTG CAGCATGCTC AAAGATTTTC 
AAGGCAACTT TGCTCAAGGA TCCTAGTGTT TCTGTGAATC GTGTTGCTTG GAGCCCAGAA 
GGTTGTTTCT TCGGGGTTGC GTATTCAAAA CACATAGTGC AAATATATTC TTATAATGAT 
GCCAAAGATG TACAGCAGAA ATTGGAGATT GATGCTCATG TTGGTGGTGT AAATGACCTA 
GCATTCTCTG CCCCTGAGAA ACAACTGTTG GTCATTACAT GTGGTGATGA CAAGACAGTT 
AAGGCATGGG ATGTGACCAG TGGTGTCAAA ATGTATTCTT TTGAAGGCCA TGATGCTCCT 
GTTTATTCTC TCTGTCCTCA TAGCAAGGGA AACGTTCATT TTCTCTCTGC AACATCAGTG 
AACAGCAACA TAAAAGTGTG CCTATATGAT AATCTAGGAG CTAAAGTAGA TTATGATGCT 
CCTGGCCTGG GGTGTACATC AATGGCTTAT AGTGGTGATA GAAGGCTTTT TTCATGTGGG 
ACCAGTAAAT CTGGAGAGTC ATTTCTTGCT GAATGGGGTG ACTCTGAAGG TTCCATAAAA 
AGAACCTATC TAGGATTGCA GAAGAGTTCT TCGGGTGTAG TGCAGTTTGA TATAATGAAG 
AACCAGGTTT TAGCTGCTGG TGATGAGCAT GTGATCAAAT TATGGGATAT GGACAAAGTT 
GAGCTTTTCA CAACCATTGA TGCTGAGGGA GGCTTACCAG AAAACCCTCG TGTTCGATTC 
AATAAGGAAG GCACGCTGTT AGCTGTTTCT GCAAATGACA ACAAAATCAA GATCTTGGCA 
AAAGATAGCG GTCTCCATTC ACTGCATACA TCACAAAATT GTTCTGATGA TGCTTCTAGA 
GATCTCTGTC ATAACTTTAA GAAGCTTGGT ATTGAACCGA GTTCAACTGT TGCTTGTGCT 
GGAGCTGCAG ATGAAGCTGT CACAAACAAT GGAAACCCAG AAAACTCGGA GGTAGTGAAA 
TCTAAAATTA CTGGAAAATC CACTACATCA AAGAGCGGGA GGCTCATTTT AATAACAAGC 
CCTTCTCAGT TCCAGATTCT GCGACTTCCT TCTCCCATGA AAGCAAACAA GATATCGAGG 
CTCATTTACA ACAATGCGGG TAACTCCATT TTGGCATTAA CATCAAATGC CTCCCATTTA 
TGTTGGAAAT GGTCACAGAA TGACACTCAC TCGAGTGACA AGGCAACTGC AAAAGTTCCC 
CCTCAACTGT GGCAACCCAG TAGTAGCTCA GGACTGATGA CAAATGACCT TACAGGCAGC 
AGCCCTGAAG AGGCAGTTCC CTGCTTTGCC CTGTCTAAGA ATGATTCATA TCTCCTGTCA 
GCATGTGGAG GGAGAATTTC TTTGTATAGC TTGCTGAAAT TCAAGACAAT GCTGCCTATC 
ATGCAACCAC CTGCAGCAAC ATGTATTGCT TTCTACCCTC AAGATAACAA CATACTTGCT 
ATAGGGAGAG ATGATTCCAC GATTCTAATT TATAATGTCC GTTCAGCTAA GGTCGATACC 
ATACTTGAGG GTCATTCTAA AAGAGTCTCT GGTCTTGCAT TCTCTAATGA TCTGAATGTA 
CTTGTTTCTT CTGGAGCAGA TGCTCAGATT TTTGTCTGGA AAGTTGAAGG GTGGGGAAAA 
GAGAGGAGCA GATTCTTGCA GATTCCCGAT GACAGAACAC TTTCTTCATT ATCATTAGAT 
ACAGACATCC AGTTTCATCA GAATCAGACA GAATTCCTTG CAGTACATGA GACTTGCCTT 
TCAATCTATG ACGCCAGAAA ATTAGAGTGT GTAAAGCAGT GGAGTCCAGG CGATTTTGGA 
GCACCAATCT CCCATGCAAC ATTCTCGTGT GATGGCCAGA TGGTGTATGC TAGCTTTGAA 
GATGGGCTTG TATCTATATT TGATGCTTCG GATTTTCAGT TATACTGTCG GATTAATCCA 
ACTGCTTACC TTTCTCCCAC TTCAAGTTTG GGAGTGTATC CGCTTGTCGT TGCCGCACAT 
CCTCAGGAAC CTGACCAGTT TGCAGTGGGA CTGAAAGATG GTGCTGTTAT TGTCTTTGAG 
CCTCCAATTT CTGCCGGTAA ATGGAGTATG CTGACAGCAT ATGAAAACGG ATCAGCAAGT 
AAAATACCTG CTGAATCCGA AGCTAATCAG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024459723.1
CDS	272..3544
Translation	MSALSKDLVLLISQFLDEEGFKETARMLERESSYYFNMKFFEDMICSGDWDEAERYFSCFTKLTDNRFSMKVYFEIRKQKFLEALDNKDRAKALDILVKDLKTFVSYNEELFKEMTLLLTLNDIRDHESLSMYSDADSARKVMRVELKKLIEANPLFSDKLEFPNAASHRLRRLINQSLNWQHVLCAYPQPNPDIRTLFVDHVCVPIPSDDHLFSTLSDSNPVPSQTTSMLVSTSSASNSTSSSEAHSSISSEALSLGDPTNIGIATITDGSEDITSVHYSSIPESELAILKRPSDEEISADSHPDQSSIDISDDLPKNVLRILNEGSSPTSMDFHPEHQTVLLVGTSVGDIGLWEVRSGESLLSRNFKVWDIAACSKIFKATLLKDPSVSVNRVAWSPEGCFFGVAYSKHIVQIYSYNDAKDVQQKLEIDAHVGGVNDLAFSAPEKQLLVITCGDDKTVKAWDVTSGVKMYSFEGHDAPVYSLCPHSKGNVHFLSATSVNSNIKVCLYDNLGAKVDYDAPGLGCTSMAYSGDRRLFSCGTSKSGESFLAEWGDSEGSIKRTYLGLQKSSSGVVQFDIMKNQVLAAGDEHVIKLWDMDKVELFTTIDAEGGLPENPRVRFNKEGTLLAVSANDNKIKILAKDSGLHSLHTSQNCSDDASRDLCHNFKKLGIEPSSTVACAGAADEAVTNNGNPENSEVVKSKITGKSTTSKSGRLILITSPSQFQILRLPSPMKANKISRLIYNNAGNSILALTSNASHLCWKWSQNDTHSSDKATAKVPPQLWQPSSSSGLMTNDLTGSSPEEAVPCFALSKNDSYLLSACGGRISLYSLLKFKTMLPIMQPPAATCIAFYPQDNNILAIGRDDSTILIYNVRSAKVDTILEGHSKRVSGLAFSNDLNVLVSSGADAQIFVWKVEGWGKERSRFLQIPDDRTLSSLSLDTDIQFHQNQTEFLAVHETCLSIYDARKLECVKQWSPGDFGAPISHATFSCDGQMVYASFEDGLVSIFDASDFQLYCRINPTAYLSPTSSLGVYPLVVAAHPQEPDQFAVGLKDGAVIVFEPPISAGKWSMLTAYENGSASKIPAESEANQ

Target ORF information:

RefSeq Version	XM_024603955.1
Organism	Populus trichocarpa(black cottonwood)
Definition	Populus trichocarpa topless-related protein 1 (LOC7489126), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024603955.1

ORF Insert Sequence:

ATGTCTGCTC TCAGTAAAGA TCTTGTCTTG TTAATCTCAC AGTTCTTAGA TGAGGAAGGG 
TTCAAAGAAA CTGCTCGCAT GCTTGAGCGT GAAAGTAGTT ATTACTTCAA CATGAAGTTT 
TTTGAGGATA TGATATGCAG TGGTGATTGG GATGAGGCTG AGAGATATTT TTCTTGTTTC 
ACAAAATTAA CTGACAATAG ATTCTCGATG AAGGTCTATT TTGAAATTAG GAAGCAGAAA 
TTTCTGGAGG CATTGGACAA CAAGGACCGT GCCAAGGCTT TAGACATCCT TGTGAAGGAT 
TTGAAAACTT TTGTCTCATA TAATGAGGAG CTGTTTAAGG AAATGACTTT GCTTTTGACA 
TTGAATGATA TAAGGGACCA TGAGTCACTC TCCATGTATA GTGATGCAGA CTCTGCAAGA 
AAAGTTATGA GGGTTGAGCT TAAGAAACTT ATTGAAGCAA ACCCCCTTTT CAGCGACAAA 
TTGGAATTTC CGAATGCTGC AAGTCATAGA TTACGACGCC TCATAAACCA AAGTCTGAAT 
TGGCAGCATG TTCTTTGTGC ATATCCACAG CCAAATCCTG ATATAAGAAC CCTTTTTGTG 
GATCATGTCT GTGTACCAAT TCCATCAGAT GATCATTTAT TTTCCACATT GAGTGATAGC 
AATCCAGTGC CTTCTCAAAC CACATCAATG CTAGTTTCAA CTTCCTCAGC CTCAAATTCT 
ACCTCATCTT CTGAGGCTCA CTCTTCAATT TCAAGTGAAG CTTTGTCTCT CGGTGATCCA 
ACAAACATAG GAATTGCTAC TATTACAGAT GGTTCGGAGG ATATTACTAG TGTACATTAC 
AGTAGTATTC CTGAAAGTGA ACTTGCAATC CTAAAAAGGC CTTCTGACGA GGAAATATCA 
GCTGACTCTC ATCCTGATCA AAGCTCCATC GACATTTCTG ATGACTTGCC TAAGAATGTT 
TTAAGGATAT TAAATGAGGG GTCATCTCCA ACAAGCATGG ATTTTCACCC TGAACATCAG 
ACTGTTCTTC TAGTTGGAAC TTCTGTGGGG GACATAGGAT TGTGGGAAGT TAGATCAGGG 
GAAAGTTTAC TTTCAAGGAA TTTTAAGGTC TGGGATATTG CAGCATGCTC AAAGATTTTC 
AAGGCAACTT TGCTCAAGGA TCCTAGTGTT TCTGTGAATC GTGTTGCTTG GAGCCCAGAA 
GGTTGTTTCT TCGGGGTTGC GTATTCAAAA CACATAGTGC AAATATATTC TTATAATGAT 
GCCAAAGATG TACAGCAGAA ATTGGAGATT GATGCTCATG TTGGTGGTGT AAATGACCTA 
GCATTCTCTG CCCCTGAGAA ACAACTGTTG GTCATTACAT GTGGTGATGA CAAGACAGTT 
AAGGCATGGG ATGTGACCAG TGGTGTCAAA ATGTATTCTT TTGAAGGCCA TGATGCTCCT 
GTTTATTCTC TCTGTCCTCA TAGCAAGGGA AACGTTCATT TTCTCTCTGC AACATCAGTG 
AACAGCAACA TAAAAGTGTG CCTATATGAT AATCTAGGAG CTAAAGTAGA TTATGATGCT 
CCTGGCCTGG GGTGTACATC AATGGCTTAT AGTGGTGATA GAAGGCTTTT TTCATGTGGG 
ACCAGTAAAT CTGGAGAGTC ATTTCTTGCT GAATGGGGTG ACTCTGAAGG TTCCATAAAA 
AGAACCTATC TAGGATTGCA GAAGAGTTCT TCGGGTGTAG TGCAGTTTGA TATAATGAAG 
AACCAGGTTT TAGCTGCTGG TGATGAGCAT GTGATCAAAT TATGGGATAT GGACAAAGTT 
GAGCTTTTCA CAACCATTGA TGCTGAGGGA GGCTTACCAG AAAACCCTCG TGTTCGATTC 
AATAAGGAAG GCACGCTGTT AGCTGTTTCT GCAAATGACA ACAAAATCAA GATCTTGGCA 
AAAGATAGCG GTCTCCATTC ACTGCATACA TCACAAAATT GTTCTGATGA TGCTTCTAGA 
GATCTCTGTC ATAACTTTAA GAAGCTTGGT ATTGAACCGA GTTCAACTGT TGCTTGTGCT 
GGAGCTGCAG ATGAAGCTGT CACAAACAAT GGAAACCCAG AAAACTCGGA GGTAGTGAAA 
TCTAAAATTA CTGGAAAATC CACTACATCA AAGAGCGGGA GGCTCATTTT AATAACAAGC 
CCTTCTCAGT TCCAGATTCT GCGACTTCCT TCTCCCATGA AAGCAAACAA GATATCGAGG 
CTCATTTACA ACAATGCGGG TAACTCCATT TTGGCATTAA CATCAAATGC CTCCCATTTA 
TGTTGGAAAT GGTCACAGAA TGACACTCAC TCGAGTGACA AGGCAACTGC AAAAGTTCCC 
CCTCAACTGT GGCAACCCAG TAGTAGCTCA GGACTGATGA CAAATGACCT TACAGGCAGC 
AGCCCTGAAG AGGCAGTTCC CTGCTTTGCC CTGTCTAAGA ATGATTCATA TCTCCTGTCA 
GCATGTGGAG GGAGAATTTC TTTGTATAGC TTGCTGAAAT TCAAGACAAT GCTGCCTATC 
ATGCAACCAC CTGCAGCAAC ATGTATTGCT TTCTACCCTC AAGATAACAA CATACTTGCT 
ATAGGGAGAG ATGATTCCAC GATTCTAATT TATAATGTCC GTTCAGCTAA GGTCGATACC 
ATACTTGAGG GTCATTCTAA AAGAGTCTCT GGTCTTGCAT TCTCTAATGA TCTGAATGTA 
CTTGTTTCTT CTGGAGCAGA TGCTCAGATT TTTGTCTGGA AAGTTGAAGG GTGGGGAAAA 
GAGAGGAGCA GATTCTTGCA GATTCCCGAT GACAGAACAC TTTCTTCATT ATCATTAGAT 
ACAGACATCC AGTTTCATCA GAATCAGACA GAATTCCTTG CAGTACATGA GACTTGCCTT 
TCAATCTATG ACGCCAGAAA ATTAGAGTGT GTAAAGCAGT GGAGTCCAGG CGATTTTGGA 
GCACCAATCT CCCATGCAAC ATTCTCGTGT GATGGCCAGA TGGTGTATGC TAGCTTTGAA 
GATGGGCTTG TATCTATATT TGATGCTTCG GATTTTCAGT TATACTGTCG GATTAATCCA 
ACTGCTTACC TTTCTCCCAC TTCAAGTTTG GGAGTGTATC CGCTTGTCGT TGCCGCACAT 
CCTCAGGAAC CTGACCAGTT TGCAGTGGGA CTGAAAGATG GTGCTGTTAT TGTCTTTGAG 
CCTCCAATTT CTGCCGGTAA ATGGAGTATG CTGACAGCAT ATGAAAACGG ATCAGCAAGT 
AAAATACCTG CTGAATCCGA AGCTAATCAG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

POPTR_0006s27430g ( XM_024603956.1 ) cDNA ORF clone, Populus trichocarpa(Populus balsamifera subsp. trichocarpa) -> XP_024459724.1 Populus trichocarpa topless-related protein 1 (LOC7489126), transcript variant X2, mRNA.

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CloneID	OPa432165
Clone ID Related Accession (Same CDS sequence)	XM_024603956.1
Accession Version	XM_024603956.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3261bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-04-08
Organism	Populus trichocarpa(black cottonwood)
Product	topless-related protein 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037290.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Populus trichocarpa Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGCTC TCAGTAAAGA TCTTGTCTTG TTAATCTCAC AGTTCTTAGA TGAGGAAGGG 
TTCAAAGAAA CTGCTCGCAT GCTTGAGCGT GAAAGTAGTT ATTACTTCAA CATGAAGTTT 
TTTGAGGATA TGATATGCAG TGGTGATTGG GATGAGGCTG AGAGATATTT TTCTTGTTTC 
ACAAAATTAA CTGACAATAG ATTCTCGATG AAGGTCTATT TTGAAATTAG GAAGCAGAAA 
TTTCTGGAGG CATTGGACAA CAAGGACCGT GCCAAGGCTT TAGACATCCT TGTGAAGGAT 
TTGAAAACTT TTGTCTCATA TAATGAGGAG CTGTTTAAGG AAATGACTTT GCTTTTGACA 
TTGAATGATA TAAGGGACCA TGAGTCACTC TCCATGTATA GTGATGCAGA CTCTGCAAGA 
AAAGTTATGA GGGTTGAGCT TAAGAAACTT ATTGAAGCAA ACCCCCTTTT CAGCGACAAA 
TTGGAATTTC CGAATGCTGC AAGTCATAGA TTACGACGCC TCATAAACCA AAGTCTGAAT 
TGGCAGCATG TTCTTTGTGC ATATCCACAG CCAAATCCTG ATATAAGAAC CCTTTTTGTG 
GATCATGTCT GTGTACCAAT TCCATCAGAT GATCATTTAT TTTCCACATT GAGTGATAGC 
AATCCAGTGC CTTCTCAAAC CACATCAATG CTAGTTTCAA CTTCCTCAGC CTCAAATTCT 
ACCTCATCTT CTGAGGCTCA CTCTTCAATT TCAAGTGAAG CTTTGTCTCT CGGTGATCCA 
ACAAACATAG GAATTGATGG TTCGGAGGAT ATTACTAGTG TACATTACAG TAGTATTCCT 
GAAAGTGAAC TTGCAATCCT AAAAAGGCCT TCTGACGAGG AAATATCAGC TGACTCTCAT 
CCTGATCAAA GCTCCATCGA CATTTCTGAT GACTTGCCTA AGAATGTTTT AAGGATATTA 
AATGAGGGGT CATCTCCAAC AAGCATGGAT TTTCACCCTG AACATCAGAC TGTTCTTCTA 
GTTGGAACTT CTGTGGGGGA CATAGGATTG TGGGAAGTTA GATCAGGGGA AAGTTTACTT 
TCAAGGAATT TTAAGGTCTG GGATATTGCA GCATGCTCAA AGATTTTCAA GGCAACTTTG 
CTCAAGGATC CTAGTGTTTC TGTGAATCGT GTTGCTTGGA GCCCAGAAGG TTGTTTCTTC 
GGGGTTGCGT ATTCAAAACA CATAGTGCAA ATATATTCTT ATAATGATGC CAAAGATGTA 
CAGCAGAAAT TGGAGATTGA TGCTCATGTT GGTGGTGTAA ATGACCTAGC ATTCTCTGCC 
CCTGAGAAAC AACTGTTGGT CATTACATGT GGTGATGACA AGACAGTTAA GGCATGGGAT 
GTGACCAGTG GTGTCAAAAT GTATTCTTTT GAAGGCCATG ATGCTCCTGT TTATTCTCTC 
TGTCCTCATA GCAAGGGAAA CGTTCATTTT CTCTCTGCAA CATCAGTGAA CAGCAACATA 
AAAGTGTGCC TATATGATAA TCTAGGAGCT AAAGTAGATT ATGATGCTCC TGGCCTGGGG 
TGTACATCAA TGGCTTATAG TGGTGATAGA AGGCTTTTTT CATGTGGGAC CAGTAAATCT 
GGAGAGTCAT TTCTTGCTGA ATGGGGTGAC TCTGAAGGTT CCATAAAAAG AACCTATCTA 
GGATTGCAGA AGAGTTCTTC GGGTGTAGTG CAGTTTGATA TAATGAAGAA CCAGGTTTTA 
GCTGCTGGTG ATGAGCATGT GATCAAATTA TGGGATATGG ACAAAGTTGA GCTTTTCACA 
ACCATTGATG CTGAGGGAGG CTTACCAGAA AACCCTCGTG TTCGATTCAA TAAGGAAGGC 
ACGCTGTTAG CTGTTTCTGC AAATGACAAC AAAATCAAGA TCTTGGCAAA AGATAGCGGT 
CTCCATTCAC TGCATACATC ACAAAATTGT TCTGATGATG CTTCTAGAGA TCTCTGTCAT 
AACTTTAAGA AGCTTGGTAT TGAACCGAGT TCAACTGTTG CTTGTGCTGG AGCTGCAGAT 
GAAGCTGTCA CAAACAATGG AAACCCAGAA AACTCGGAGG TAGTGAAATC TAAAATTACT 
GGAAAATCCA CTACATCAAA GAGCGGGAGG CTCATTTTAA TAACAAGCCC TTCTCAGTTC 
CAGATTCTGC GACTTCCTTC TCCCATGAAA GCAAACAAGA TATCGAGGCT CATTTACAAC 
AATGCGGGTA ACTCCATTTT GGCATTAACA TCAAATGCCT CCCATTTATG TTGGAAATGG 
TCACAGAATG ACACTCACTC GAGTGACAAG GCAACTGCAA AAGTTCCCCC TCAACTGTGG 
CAACCCAGTA GTAGCTCAGG ACTGATGACA AATGACCTTA CAGGCAGCAG CCCTGAAGAG 
GCAGTTCCCT GCTTTGCCCT GTCTAAGAAT GATTCATATC TCCTGTCAGC ATGTGGAGGG 
AGAATTTCTT TGTATAGCTT GCTGAAATTC AAGACAATGC TGCCTATCAT GCAACCACCT 
GCAGCAACAT GTATTGCTTT CTACCCTCAA GATAACAACA TACTTGCTAT AGGGAGAGAT 
GATTCCACGA TTCTAATTTA TAATGTCCGT TCAGCTAAGG TCGATACCAT ACTTGAGGGT 
CATTCTAAAA GAGTCTCTGG TCTTGCATTC TCTAATGATC TGAATGTACT TGTTTCTTCT 
GGAGCAGATG CTCAGATTTT TGTCTGGAAA GTTGAAGGGT GGGGAAAAGA GAGGAGCAGA 
TTCTTGCAGA TTCCCGATGA CAGAACACTT TCTTCATTAT CATTAGATAC AGACATCCAG 
TTTCATCAGA ATCAGACAGA ATTCCTTGCA GTACATGAGA CTTGCCTTTC AATCTATGAC 
GCCAGAAAAT TAGAGTGTGT AAAGCAGTGG AGTCCAGGCG ATTTTGGAGC ACCAATCTCC 
CATGCAACAT TCTCGTGTGA TGGCCAGATG GTGTATGCTA GCTTTGAAGA TGGGCTTGTA 
TCTATATTTG ATGCTTCGGA TTTTCAGTTA TACTGTCGGA TTAATCCAAC TGCTTACCTT 
TCTCCCACTT CAAGTTTGGG AGTGTATCCG CTTGTCGTTG CCGCACATCC TCAGGAACCT 
GACCAGTTTG CAGTGGGACT GAAAGATGGT GCTGTTATTG TCTTTGAGCC TCCAATTTCT 
GCCGGTAAAT GGAGTATGCT GACAGCATAT GAAAACGGAT CAGCAAGTAA AATACCTGCT 
GAATCCGAAG CTAATCAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024459724.1
CDS	272..3532
Translation	MSALSKDLVLLISQFLDEEGFKETARMLERESSYYFNMKFFEDMICSGDWDEAERYFSCFTKLTDNRFSMKVYFEIRKQKFLEALDNKDRAKALDILVKDLKTFVSYNEELFKEMTLLLTLNDIRDHESLSMYSDADSARKVMRVELKKLIEANPLFSDKLEFPNAASHRLRRLINQSLNWQHVLCAYPQPNPDIRTLFVDHVCVPIPSDDHLFSTLSDSNPVPSQTTSMLVSTSSASNSTSSSEAHSSISSEALSLGDPTNIGIDGSEDITSVHYSSIPESELAILKRPSDEEISADSHPDQSSIDISDDLPKNVLRILNEGSSPTSMDFHPEHQTVLLVGTSVGDIGLWEVRSGESLLSRNFKVWDIAACSKIFKATLLKDPSVSVNRVAWSPEGCFFGVAYSKHIVQIYSYNDAKDVQQKLEIDAHVGGVNDLAFSAPEKQLLVITCGDDKTVKAWDVTSGVKMYSFEGHDAPVYSLCPHSKGNVHFLSATSVNSNIKVCLYDNLGAKVDYDAPGLGCTSMAYSGDRRLFSCGTSKSGESFLAEWGDSEGSIKRTYLGLQKSSSGVVQFDIMKNQVLAAGDEHVIKLWDMDKVELFTTIDAEGGLPENPRVRFNKEGTLLAVSANDNKIKILAKDSGLHSLHTSQNCSDDASRDLCHNFKKLGIEPSSTVACAGAADEAVTNNGNPENSEVVKSKITGKSTTSKSGRLILITSPSQFQILRLPSPMKANKISRLIYNNAGNSILALTSNASHLCWKWSQNDTHSSDKATAKVPPQLWQPSSSSGLMTNDLTGSSPEEAVPCFALSKNDSYLLSACGGRISLYSLLKFKTMLPIMQPPAATCIAFYPQDNNILAIGRDDSTILIYNVRSAKVDTILEGHSKRVSGLAFSNDLNVLVSSGADAQIFVWKVEGWGKERSRFLQIPDDRTLSSLSLDTDIQFHQNQTEFLAVHETCLSIYDARKLECVKQWSPGDFGAPISHATFSCDGQMVYASFEDGLVSIFDASDFQLYCRINPTAYLSPTSSLGVYPLVVAAHPQEPDQFAVGLKDGAVIVFEPPISAGKWSMLTAYENGSASKIPAESEANQ

Target ORF information:

RefSeq Version	XM_024603956.1
Organism	Populus trichocarpa(black cottonwood)
Definition	Populus trichocarpa topless-related protein 1 (LOC7489126), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024603956.1

ORF Insert Sequence:

ATGTCTGCTC TCAGTAAAGA TCTTGTCTTG TTAATCTCAC AGTTCTTAGA TGAGGAAGGG 
TTCAAAGAAA CTGCTCGCAT GCTTGAGCGT GAAAGTAGTT ATTACTTCAA CATGAAGTTT 
TTTGAGGATA TGATATGCAG TGGTGATTGG GATGAGGCTG AGAGATATTT TTCTTGTTTC 
ACAAAATTAA CTGACAATAG ATTCTCGATG AAGGTCTATT TTGAAATTAG GAAGCAGAAA 
TTTCTGGAGG CATTGGACAA CAAGGACCGT GCCAAGGCTT TAGACATCCT TGTGAAGGAT 
TTGAAAACTT TTGTCTCATA TAATGAGGAG CTGTTTAAGG AAATGACTTT GCTTTTGACA 
TTGAATGATA TAAGGGACCA TGAGTCACTC TCCATGTATA GTGATGCAGA CTCTGCAAGA 
AAAGTTATGA GGGTTGAGCT TAAGAAACTT ATTGAAGCAA ACCCCCTTTT CAGCGACAAA 
TTGGAATTTC CGAATGCTGC AAGTCATAGA TTACGACGCC TCATAAACCA AAGTCTGAAT 
TGGCAGCATG TTCTTTGTGC ATATCCACAG CCAAATCCTG ATATAAGAAC CCTTTTTGTG 
GATCATGTCT GTGTACCAAT TCCATCAGAT GATCATTTAT TTTCCACATT GAGTGATAGC 
AATCCAGTGC CTTCTCAAAC CACATCAATG CTAGTTTCAA CTTCCTCAGC CTCAAATTCT 
ACCTCATCTT CTGAGGCTCA CTCTTCAATT TCAAGTGAAG CTTTGTCTCT CGGTGATCCA 
ACAAACATAG GAATTGATGG TTCGGAGGAT ATTACTAGTG TACATTACAG TAGTATTCCT 
GAAAGTGAAC TTGCAATCCT AAAAAGGCCT TCTGACGAGG AAATATCAGC TGACTCTCAT 
CCTGATCAAA GCTCCATCGA CATTTCTGAT GACTTGCCTA AGAATGTTTT AAGGATATTA 
AATGAGGGGT CATCTCCAAC AAGCATGGAT TTTCACCCTG AACATCAGAC TGTTCTTCTA 
GTTGGAACTT CTGTGGGGGA CATAGGATTG TGGGAAGTTA GATCAGGGGA AAGTTTACTT 
TCAAGGAATT TTAAGGTCTG GGATATTGCA GCATGCTCAA AGATTTTCAA GGCAACTTTG 
CTCAAGGATC CTAGTGTTTC TGTGAATCGT GTTGCTTGGA GCCCAGAAGG TTGTTTCTTC 
GGGGTTGCGT ATTCAAAACA CATAGTGCAA ATATATTCTT ATAATGATGC CAAAGATGTA 
CAGCAGAAAT TGGAGATTGA TGCTCATGTT GGTGGTGTAA ATGACCTAGC ATTCTCTGCC 
CCTGAGAAAC AACTGTTGGT CATTACATGT GGTGATGACA AGACAGTTAA GGCATGGGAT 
GTGACCAGTG GTGTCAAAAT GTATTCTTTT GAAGGCCATG ATGCTCCTGT TTATTCTCTC 
TGTCCTCATA GCAAGGGAAA CGTTCATTTT CTCTCTGCAA CATCAGTGAA CAGCAACATA 
AAAGTGTGCC TATATGATAA TCTAGGAGCT AAAGTAGATT ATGATGCTCC TGGCCTGGGG 
TGTACATCAA TGGCTTATAG TGGTGATAGA AGGCTTTTTT CATGTGGGAC CAGTAAATCT 
GGAGAGTCAT TTCTTGCTGA ATGGGGTGAC TCTGAAGGTT CCATAAAAAG AACCTATCTA 
GGATTGCAGA AGAGTTCTTC GGGTGTAGTG CAGTTTGATA TAATGAAGAA CCAGGTTTTA 
GCTGCTGGTG ATGAGCATGT GATCAAATTA TGGGATATGG ACAAAGTTGA GCTTTTCACA 
ACCATTGATG CTGAGGGAGG CTTACCAGAA AACCCTCGTG TTCGATTCAA TAAGGAAGGC 
ACGCTGTTAG CTGTTTCTGC AAATGACAAC AAAATCAAGA TCTTGGCAAA AGATAGCGGT 
CTCCATTCAC TGCATACATC ACAAAATTGT TCTGATGATG CTTCTAGAGA TCTCTGTCAT 
AACTTTAAGA AGCTTGGTAT TGAACCGAGT TCAACTGTTG CTTGTGCTGG AGCTGCAGAT 
GAAGCTGTCA CAAACAATGG AAACCCAGAA AACTCGGAGG TAGTGAAATC TAAAATTACT 
GGAAAATCCA CTACATCAAA GAGCGGGAGG CTCATTTTAA TAACAAGCCC TTCTCAGTTC 
CAGATTCTGC GACTTCCTTC TCCCATGAAA GCAAACAAGA TATCGAGGCT CATTTACAAC 
AATGCGGGTA ACTCCATTTT GGCATTAACA TCAAATGCCT CCCATTTATG TTGGAAATGG 
TCACAGAATG ACACTCACTC GAGTGACAAG GCAACTGCAA AAGTTCCCCC TCAACTGTGG 
CAACCCAGTA GTAGCTCAGG ACTGATGACA AATGACCTTA CAGGCAGCAG CCCTGAAGAG 
GCAGTTCCCT GCTTTGCCCT GTCTAAGAAT GATTCATATC TCCTGTCAGC ATGTGGAGGG 
AGAATTTCTT TGTATAGCTT GCTGAAATTC AAGACAATGC TGCCTATCAT GCAACCACCT 
GCAGCAACAT GTATTGCTTT CTACCCTCAA GATAACAACA TACTTGCTAT AGGGAGAGAT 
GATTCCACGA TTCTAATTTA TAATGTCCGT TCAGCTAAGG TCGATACCAT ACTTGAGGGT 
CATTCTAAAA GAGTCTCTGG TCTTGCATTC TCTAATGATC TGAATGTACT TGTTTCTTCT 
GGAGCAGATG CTCAGATTTT TGTCTGGAAA GTTGAAGGGT GGGGAAAAGA GAGGAGCAGA 
TTCTTGCAGA TTCCCGATGA CAGAACACTT TCTTCATTAT CATTAGATAC AGACATCCAG 
TTTCATCAGA ATCAGACAGA ATTCCTTGCA GTACATGAGA CTTGCCTTTC AATCTATGAC 
GCCAGAAAAT TAGAGTGTGT AAAGCAGTGG AGTCCAGGCG ATTTTGGAGC ACCAATCTCC 
CATGCAACAT TCTCGTGTGA TGGCCAGATG GTGTATGCTA GCTTTGAAGA TGGGCTTGTA 
TCTATATTTG ATGCTTCGGA TTTTCAGTTA TACTGTCGGA TTAATCCAAC TGCTTACCTT 
TCTCCCACTT CAAGTTTGGG AGTGTATCCG CTTGTCGTTG CCGCACATCC TCAGGAACCT 
GACCAGTTTG CAGTGGGACT GAAAGATGGT GCTGTTATTG TCTTTGAGCC TCCAATTTCT 
GCCGGTAAAT GGAGTATGCT GACAGCATAT GAAAACGGAT CAGCAAGTAA AATACCTGCT 
GAATCCGAAG CTAATCAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

POPTR_0006s27430g ( XM_002308645.2 ) cDNA ORF clone, Populus trichocarpa(Populus balsamifera subsp. trichocarpa) -> XP_002308681.1 Populus trichocarpa WD-40 repeat family protein (POPTR_0006s27430g) mRNA, complete cds.

Price & Availability↑

CloneID	OPa15431
Clone ID Related Accession (Same CDS sequence)	XM_002308645.2
Accession Version	XM_002308645.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3324bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2013-12-30
Organism	Populus trichocarpa(Populus balsamifera subsp. trichocarpa)
Product	WD-40 repeat family protein
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_008472). On Dec 16, 2013 this sequence version replaced XM_002308645.1.

ATGTCTGCTC TCAGTAAAGA TCTTGTCTTG TTAATCTCAC AGTTCTTAGA TGAGGAAGGG 
TTCAAAGAAA CTGCTCGCAT GCTTGAGCGT GAAAGTAGTT ATTACTTCAA CATGAAGTTT 
TTTGAGGATA TGATATGCAG TGGTGATTGG GATGAGGCTG AGAGATATTT TTCTTGTTTC 
ACAAAATTAA CTGACAATAG ATTCTCGATG AAGGTCTATT TTGAAATTAG GAAGCAGAAA 
TTTCTGGAGG CATTGGACAA CAAGGACCGT GCCAAGGCTT TAGACATCCT TGTGAAGGAT 
TTGAAAACTT TTGTCTCATA TAATGAGGAG CTGTTTAAGG AAATGACTTT GCTTTTGACA 
TTGAATGATA TAAGGGACCA TGAGTCACTC TCCATGTATA GTGATGCAGA CTCTGCAAGA 
AAAGTTATGA GGGTTGAGCT TAAGAAACTT ATTGAAGCAA ACCCCCTTTT CAGCGACAAA 
TTGGAATTTC CGAATGCTGC AAGTCATAGA TTACGACGCC TCATAAACCA AAGTCTGAAT 
TGGCAGCATG TTCTTTGTGC ATATCCACAG CCAAATCCTG ATATAAGAAC CCTTTTTGTG 
GATCATGTCT GTGTACCAAT TCCATCAGAT GATCATTTAT TTTCCACATT GAGTGATAGC 
AATCCACCTT CTCAAACCAC ATCAATGCTA GTTTCAACTT CCTCAGCCTC AAATTCTACC 
TCATCTTCTG AGGCTCACTC TTCAATTTCA AGTGAAGCTT TGTCTCTCGG TGATCCAACA 
AACATAGGAA TTGCTACTAT TACAGATGGT TCGGAGGATA TTACTAGTGT ACATTACAGT 
AGTATTCCTG AAAGTGAACT TGCAATCCTA AAAAGGCCTT CTGACGAGTC ACGGTTTCCA 
TGTATGCAGG AAATATCAGC TGACTCTCAT CCTGATCAAA GCTCCATCGA CATTTCTGAT 
GACTTGCCTA AGAATGTTTT AAGGATATTA AATGAGGGGT CATCTCCAAC AAGCATGGAT 
TTTCACCCTG AACATCAGAC TGTTCTTCTA GTTGGAACTT CTGTGGGGGA CATAGGATTG 
TGGGAAGTTA GATCAGGGGA AAGTTTACTT TCAAGGAATT TTAAGGTCTG GGATATTGCA 
GCATGCTCAA AGATTTTCAA GGCAACTTTG CTCAAGGATC CTAGTGTTTC TGTGAATCGT 
GTTGCTTGGA GCCCAGAAGG TTGTTTCTTC GGGGTTGCGT ATTCAAAACA CATAGTGCAA 
ATATATTCTT ATAATGATGC CAAAGATGTA CAGCAGAAAT TGGAGATTGA TGCTCATGTT 
GGTGGTGTAA ATGACCTAGC ATTCTCTGCC CCTGAGAAAC AACTGTTGGT CATTACATGT 
GGTGATGACA AGACAGTTAA GGCATGGGAT GTGACCAGTG GTGTCAAAAT GTATTCTTTT 
GAAGGCCATG ATGCTCCTGT TTATTCTCTC TGTCCTCATA GCAAGGGAAA CGTTCATTTT 
CTCTCTGCAA CATCAGTGAA CAGCAACATA AAAGTGTGCC TATATGATAA TCTAGGAGCT 
AAAGTAGATT ATGATGCTCC TGGCCTGGGG TGTACATCAA TGGCTTATAG TGGTGATAGA 
AGGCTTTTTT CATGTGGGAC CAGTAAATCT GGAGAGTCAT TTCTTGCTGA ATGGGGTGAC 
TCTGAAGGTT CCATAAAAAG AACCTATCTA GGATTGCAGA AGAGTTCTTC GGGTGTAGTG 
CAGTTTGATA TAATGAAGAA CCAGGTTTTA GCTGCTGGTG ATGAGCATGT GATCAAATTA 
TGGGATATGG ACAAAGTTGA GCTTTTCACA ACCATTGATG CTGAGGGAGG CTTACCAGAA 
AACCCTCGTG TTCGATTCAA TAAGGAAGGC ACGCTGTTAG CTGTTTCTGC AAATGACAAC 
AAAATCAAGA TCTTGGCAAA AGATAGCGGT CTCCATTCAC TGCATACATC ACAAAATTGT 
TCTGATGATG CTTCTAGAGA TCTCTGTCAT AACTTTAAGA AGGTGGGCAT TCTTAATACA 
GTTGTATCTT GCCTTGTCAT GACTTTGGAA TTTACTTGGA AGTTTGGAGT ACTAAGCAAT 
TTTTTTAATC TTCTGCAGAA TGGAAACCCA GAAAACTCGG AGGTAGTGAA ATCTAAAATT 
ACTGGAAAAT CCACTACATC AAAGAGCGGG AGGCTCATTT TAATAACAAG CCCTTCTCAG 
TTCCAGATTC TGCGACTTCC TTCTCCCATG AAAGCAAACA AGATATCGAG GCTCATTTAC 
AACAATGCGG GTAACTCCAT TTTGGCATTA ACATCAAATG CCTCCCATTT ATGTTGGAAA 
TGGTCACAGA ATGACACTCA CTCGAGTGAC AAGGCAACTG CAAAAGTTCC CCCTCAACTG 
TGGCAACCCA GTAGTAGCTC AGGACTGATG ACAAATGACC TTACAGGCAG CAGCCCTGAA 
GAGGCAGTTC CCTGCTTTGC CCTGTCTAAG AATGATTCAT ATCTCCTGTC AGCATGTGGA 
GGGAGAATTT CTTTGTATAG CTTGCTGAAA TTCAAGACAA TGCTGCCTAT CATGCAACCA 
CCTGCAGCAA CATGTATTGC TTTCTACCCT CAAGATAACA ACATACTTGC TATAGGGAGA 
GATGATTCCA CGATTCTAAT TTATAATGTC CGTTCAGCTA AGGTCGATAC CATACTTGAG 
GGTCATTCTA AAAGAGTCTC TGGTCTTGCA TTCTCTAATG ATCTGAATGT ACTTGTTTCT 
TCTGGAGCAG ATGCTCAGAT TTTTGTCTGG AAAGTTGAAG GGTGGGGAAA AGAGAGGAGC 
AGATTCTTGC AGATTCCCGA TGACAGAACA CTTTCTTCAT TATCATTAGA TACAGACATC 
CAGTTTCATC AGAATCAGAC AGAATTCCTT GCAGTACATG AGACTTGCCT TTCAATATAT 
GACGCCAGAA AATTAGAGTG TGTAAAGCAG TGGAGTCCAG GCGATTTTGG AGCACCAATC 
TCCCATGCAA CATTCTCGTG TGATGGCCAG ATGGTGTATG CTAGCTTTGA AGATGGGCTT 
GTATCTATAT TTGATGCTTC GGATTTTCAG TTATACTGTC GGATTAATCC AACTGCTTAC 
CTTTCTCCCA CTTCAAGTTT GGGAGTGTAT CCGCTTGTCG TTGCCGCACA TCCTCAGGAA 
CCTGACCAGT TTGCAGTGGG ACTGAAAGAT GGTGCTGTTA TTGTCTTTGA GCCTCCAATT 
TCTGCCGGTA AATGGAGTAT GCTGACAGCA TATGAAAACG GATCAGCAAG TAAAATACCT 
GCTGAATCCG AAGCTAATCA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002308681.1
CDS	232..3555
Translation	MSALSKDLVLLISQFLDEEGFKETARMLERESSYYFNMKFFEDMICSGDWDEAERYFSCFTKLTDNRFSMKVYFEIRKQKFLEALDNKDRAKALDILVKDLKTFVSYNEELFKEMTLLLTLNDIRDHESLSMYSDADSARKVMRVELKKLIEANPLFSDKLEFPNAASHRLRRLINQSLNWQHVLCAYPQPNPDIRTLFVDHVCVPIPSDDHLFSTLSDSNPPSQTTSMLVSTSSASNSTSSSEAHSSISSEALSLGDPTNIGIATITDGSEDITSVHYSSIPESELAILKRPSDESRFPCMQEISADSHPDQSSIDISDDLPKNVLRILNEGSSPTSMDFHPEHQTVLLVGTSVGDIGLWEVRSGESLLSRNFKVWDIAACSKIFKATLLKDPSVSVNRVAWSPEGCFFGVAYSKHIVQIYSYNDAKDVQQKLEIDAHVGGVNDLAFSAPEKQLLVITCGDDKTVKAWDVTSGVKMYSFEGHDAPVYSLCPHSKGNVHFLSATSVNSNIKVCLYDNLGAKVDYDAPGLGCTSMAYSGDRRLFSCGTSKSGESFLAEWGDSEGSIKRTYLGLQKSSSGVVQFDIMKNQVLAAGDEHVIKLWDMDKVELFTTIDAEGGLPENPRVRFNKEGTLLAVSANDNKIKILAKDSGLHSLHTSQNCSDDASRDLCHNFKKVGILNTVVSCLVMTLEFTWKFGVLSNFFNLLQNGNPENSEVVKSKITGKSTTSKSGRLILITSPSQFQILRLPSPMKANKISRLIYNNAGNSILALTSNASHLCWKWSQNDTHSSDKATAKVPPQLWQPSSSSGLMTNDLTGSSPEEAVPCFALSKNDSYLLSACGGRISLYSLLKFKTMLPIMQPPAATCIAFYPQDNNILAIGRDDSTILIYNVRSAKVDTILEGHSKRVSGLAFSNDLNVLVSSGADAQIFVWKVEGWGKERSRFLQIPDDRTLSSLSLDTDIQFHQNQTEFLAVHETCLSIYDARKLECVKQWSPGDFGAPISHATFSCDGQMVYASFEDGLVSIFDASDFQLYCRINPTAYLSPTSSLGVYPLVVAAHPQEPDQFAVGLKDGAVIVFEPPISAGKWSMLTAYENGSASKIPAESEANQ

Target ORF information:

RefSeq Version	XM_002308645.2
Organism	Populus trichocarpa(Populus balsamifera subsp. trichocarpa)
Definition	Populus trichocarpa WD-40 repeat family protein (POPTR_0006s27430g) mRNA, complete cds.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002308645.2

ORF Insert Sequence:

ATGTCTGCTC TCAGTAAAGA TCTTGTCTTG TTAATCTCAC AGTTCTTAGA TGAGGAAGGG 
TTCAAAGAAA CTGCTCGCAT GCTTGAGCGT GAAAGTAGTT ATTACTTCAA CATGAAGTTT 
TTTGAGGATA TGATATGCAG TGGTGATTGG GATGAGGCTG AGAGATATTT TTCTTGTTTC 
ACAAAATTAA CTGACAATAG ATTCTCGATG AAGGTCTATT TTGAAATTAG GAAGCAGAAA 
TTTCTGGAGG CATTGGACAA CAAGGACCGT GCCAAGGCTT TAGACATCCT TGTGAAGGAT 
TTGAAAACTT TTGTCTCATA TAATGAGGAG CTGTTTAAGG AAATGACTTT GCTTTTGACA 
TTGAATGATA TAAGGGACCA TGAGTCACTC TCCATGTATA GTGATGCAGA CTCTGCAAGA 
AAAGTTATGA GGGTTGAGCT TAAGAAACTT ATTGAAGCAA ACCCCCTTTT CAGCGACAAA 
TTGGAATTTC CGAATGCTGC AAGTCATAGA TTACGACGCC TCATAAACCA AAGTCTGAAT 
TGGCAGCATG TTCTTTGTGC ATATCCACAG CCAAATCCTG ATATAAGAAC CCTTTTTGTG 
GATCATGTCT GTGTACCAAT TCCATCAGAT GATCATTTAT TTTCCACATT GAGTGATAGC 
AATCCACCTT CTCAAACCAC ATCAATGCTA GTTTCAACTT CCTCAGCCTC AAATTCTACC 
TCATCTTCTG AGGCTCACTC TTCAATTTCA AGTGAAGCTT TGTCTCTCGG TGATCCAACA 
AACATAGGAA TTGCTACTAT TACAGATGGT TCGGAGGATA TTACTAGTGT ACATTACAGT 
AGTATTCCTG AAAGTGAACT TGCAATCCTA AAAAGGCCTT CTGACGAGTC ACGGTTTCCA 
TGTATGCAGG AAATATCAGC TGACTCTCAT CCTGATCAAA GCTCCATCGA CATTTCTGAT 
GACTTGCCTA AGAATGTTTT AAGGATATTA AATGAGGGGT CATCTCCAAC AAGCATGGAT 
TTTCACCCTG AACATCAGAC TGTTCTTCTA GTTGGAACTT CTGTGGGGGA CATAGGATTG 
TGGGAAGTTA GATCAGGGGA AAGTTTACTT TCAAGGAATT TTAAGGTCTG GGATATTGCA 
GCATGCTCAA AGATTTTCAA GGCAACTTTG CTCAAGGATC CTAGTGTTTC TGTGAATCGT 
GTTGCTTGGA GCCCAGAAGG TTGTTTCTTC GGGGTTGCGT ATTCAAAACA CATAGTGCAA 
ATATATTCTT ATAATGATGC CAAAGATGTA CAGCAGAAAT TGGAGATTGA TGCTCATGTT 
GGTGGTGTAA ATGACCTAGC ATTCTCTGCC CCTGAGAAAC AACTGTTGGT CATTACATGT 
GGTGATGACA AGACAGTTAA GGCATGGGAT GTGACCAGTG GTGTCAAAAT GTATTCTTTT 
GAAGGCCATG ATGCTCCTGT TTATTCTCTC TGTCCTCATA GCAAGGGAAA CGTTCATTTT 
CTCTCTGCAA CATCAGTGAA CAGCAACATA AAAGTGTGCC TATATGATAA TCTAGGAGCT 
AAAGTAGATT ATGATGCTCC TGGCCTGGGG TGTACATCAA TGGCTTATAG TGGTGATAGA 
AGGCTTTTTT CATGTGGGAC CAGTAAATCT GGAGAGTCAT TTCTTGCTGA ATGGGGTGAC 
TCTGAAGGTT CCATAAAAAG AACCTATCTA GGATTGCAGA AGAGTTCTTC GGGTGTAGTG 
CAGTTTGATA TAATGAAGAA CCAGGTTTTA GCTGCTGGTG ATGAGCATGT GATCAAATTA 
TGGGATATGG ACAAAGTTGA GCTTTTCACA ACCATTGATG CTGAGGGAGG CTTACCAGAA 
AACCCTCGTG TTCGATTCAA TAAGGAAGGC ACGCTGTTAG CTGTTTCTGC AAATGACAAC 
AAAATCAAGA TCTTGGCAAA AGATAGCGGT CTCCATTCAC TGCATACATC ACAAAATTGT 
TCTGATGATG CTTCTAGAGA TCTCTGTCAT AACTTTAAGA AGGTGGGCAT TCTTAATACA 
GTTGTATCTT GCCTTGTCAT GACTTTGGAA TTTACTTGGA AGTTTGGAGT ACTAAGCAAT 
TTTTTTAATC TTCTGCAGAA TGGAAACCCA GAAAACTCGG AGGTAGTGAA ATCTAAAATT 
ACTGGAAAAT CCACTACATC AAAGAGCGGG AGGCTCATTT TAATAACAAG CCCTTCTCAG 
TTCCAGATTC TGCGACTTCC TTCTCCCATG AAAGCAAACA AGATATCGAG GCTCATTTAC 
AACAATGCGG GTAACTCCAT TTTGGCATTA ACATCAAATG CCTCCCATTT ATGTTGGAAA 
TGGTCACAGA ATGACACTCA CTCGAGTGAC AAGGCAACTG CAAAAGTTCC CCCTCAACTG 
TGGCAACCCA GTAGTAGCTC AGGACTGATG ACAAATGACC TTACAGGCAG CAGCCCTGAA 
GAGGCAGTTC CCTGCTTTGC CCTGTCTAAG AATGATTCAT ATCTCCTGTC AGCATGTGGA 
GGGAGAATTT CTTTGTATAG CTTGCTGAAA TTCAAGACAA TGCTGCCTAT CATGCAACCA 
CCTGCAGCAA CATGTATTGC TTTCTACCCT CAAGATAACA ACATACTTGC TATAGGGAGA 
GATGATTCCA CGATTCTAAT TTATAATGTC CGTTCAGCTA AGGTCGATAC CATACTTGAG 
GGTCATTCTA AAAGAGTCTC TGGTCTTGCA TTCTCTAATG ATCTGAATGT ACTTGTTTCT 
TCTGGAGCAG ATGCTCAGAT TTTTGTCTGG AAAGTTGAAG GGTGGGGAAA AGAGAGGAGC 
AGATTCTTGC AGATTCCCGA TGACAGAACA CTTTCTTCAT TATCATTAGA TACAGACATC 
CAGTTTCATC AGAATCAGAC AGAATTCCTT GCAGTACATG AGACTTGCCT TTCAATATAT 
GACGCCAGAA AATTAGAGTG TGTAAAGCAG TGGAGTCCAG GCGATTTTGG AGCACCAATC 
TCCCATGCAA CATTCTCGTG TGATGGCCAG ATGGTGTATG CTAGCTTTGA AGATGGGCTT 
GTATCTATAT TTGATGCTTC GGATTTTCAG TTATACTGTC GGATTAATCC AACTGCTTAC 
CTTTCTCCCA CTTCAAGTTT GGGAGTGTAT CCGCTTGTCG TTGCCGCACA TCCTCAGGAA 
CCTGACCAGT TTGCAGTGGG ACTGAAAGAT GGTGCTGTTA TTGTCTTTGA GCCTCCAATT 
TCTGCCGGTA AATGGAGTAT GCTGACAGCA TATGAAAACG GATCAGCAAG TAAAATACCT 
GCTGAATCCG AAGCTAATCA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The genome of black cottonwood, Populus trichocarpa (Torr. & Gray).
Science (New York, N.Y.)313(5793)1596-604(2006 Sep)
Tuskan GA,Difazio S,Jansson S,Bohlmann J,Grigoriev I,Hellsten U,Putnam N,Ralph S,Rombauts S,Salamov A,Schein J,Sterck L,Aerts A,Bhalerao RR,Bhalerao RP,Blaudez D,Boerjan W,Brun A,Brunner A,Busov V,Campbell M,Carlson J,Chalot M,Chapman J,Chen GL,Cooper D,Coutinho PM,Couturier J,Covert S,Cronk Q,Cunningham R,Davis J,Degroeve S,D?jardin A,Depamphilis C,Detter J,Dirks B,Dubchak I,Duplessis S,Ehlting J,Ellis B,Gendler K,Goodstein D,Gribskov M,Grimwood J,Groover A,Gunter L,Hamberger B,Heinze B,Helariutta Y,Henrissat B,Holligan D,Holt R,Huang W,Islam-Faridi N,Jones S,Jones-Rhoades M,Jorgensen R,Joshi C,Kangasj?rvi J,Karlsson J,Kelleher C,Kirkpatrick R,Kirst M,Kohler A,Kalluri U,Larimer F,Leebens-Mack J,Lepl? JC,Locascio P,Lou Y,Lucas S,Martin F,Montanini B,Napoli C,Nelson DR,Nelson C,Nieminen K,Nilsson O,Pereda V,Peter G,Philippe R,Pilate G,Poliakov A,Razumovskaya J,Richardson P,Rinaldi C,Ritland K,Rouz? P,Ryaboy D,Schmutz J,Schrader J,Segerman B,Shin H,Siddiqui A,Sterky F,Terry A,Tsai CJ,Uberbacher E,Unneberg P,Vahala J,Wall K,Wessler S,Yang G,Yin T,Douglas C,Marra M,Sandberg G,Van de Peer Y,Rokhsar D

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POPTR_0006s27430g cDNA ORF clone, Populus trichocarpa(Populus balsamifera subsp. trichocarpa)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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