LOC18096402 cDNA ORF clone, Populus trichocarpa(black cottonwood)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC18096402
Entrez Gene ID	18096402
Full Name	Endoribonuclease Dicer family protein
Gene Type	protein-coding
Organism	Populus trichocarpa(black cottonwood)

mRNA and Protein(s)

mRNA	Protein	Name
XM_006386606.1	XP_006386668.1	Endoribonuclease Dicer family protein
XM_024595813.1	XP_024451581.1	endoribonuclease Dicer homolog 1 isoform X1
XM_024595814.1	XP_024451582.1	endoribonuclease Dicer homolog 1 isoform X1
XM_006386606.2	XP_006386668.1	endoribonuclease Dicer homolog 1 isoform X2

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

The genome of black cottonwood, Populus trichocarpa (Torr. & Gray).
Tuskan GA, Difazio S, Jansson S, Bohlmann J, Grigoriev I, Hellsten U, Putnam N, Ralph S, Rombauts S, Salamov A, Schein J, Sterck L, Aerts A, Bhalerao RR, Bhalerao RP, Blaudez D, Boerjan W, Brun A, Brunner A, Busov V, Campbell M, Carlson J, Chalot M, Chapman J, Chen GL, Cooper D, Coutinho PM, Couturier J, Covert S, Cronk Q, Cunningham R, Davis J, Degroeve S, D?jardin A, Depamphilis C, Detter J, Dirks B, Dubchak I, Duplessis S, Ehlting J, Ellis B, Gendler K, Goodstein D, Gribskov M, Grimwood J, Groover A, Gunter L, Hamberger B, Heinze B, Helariutta Y, Henrissat B, Holligan D, Holt R, Huang W, Islam-Faridi N, Jones S, Jones-Rhoades M, Jorgensen R, Joshi C, Kangasj?rvi J, Karlsson J, Kelleher C, Kirkpatrick R, Kirst M, Kohler A, Kalluri U, Larimer F, Leebens-Mack J, Lepl? JC, Locascio P, Lou Y, Lucas S, Martin F, Montanini B, Napoli C, Nelson DR, Nelson C, Nieminen K, Nilsson O, Pereda V, Peter G, Philippe R, Pilate G, Poliakov A, Razumovskaya J, Richardson P, Rinaldi C, Ritland K, Rouz? P, Ryaboy D, Schmutz J, Schrader J, Segerman B, Shin H, Siddiqui A, Sterky F, Terry A, Tsai CJ, Uberbacher E, Unneberg P, Vahala J, Wall K, Wessler S, Yang G, Yin T, Douglas C, Marra M, Sandberg G, Van de Peer Y, Rokhsar D
Science (New York, N.Y.)313(5793)1596-604(2006 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following LOC18096402 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC18096402 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OPa425075	XM_024595813.1 Latest version!	Populus trichocarpa endoribonuclease Dicer homolog 1 (LOC18096402), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OPa425075	XM_024595814.1 Latest version!	Populus trichocarpa endoribonuclease Dicer homolog 1 (LOC18096402), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OPa07043	XM_006386606.2 Latest version!	Populus trichocarpa endoribonuclease Dicer homolog 1 (LOC18096402), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
View Old Accession Versions >>
OPa07043	XM_006386606.1	Populus trichocarpa Endoribonuclease Dicer family protein (POPTR_0002s18240g) mRNA, complete cds.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC18096402 ( XM_024595813.1 ) cDNA ORF clone, Populus trichocarpa(black cottonwood) -> XP_024451581.1 Populus trichocarpa endoribonuclease Dicer homolog 1 (LOC18096402), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OPa425075
Clone ID Related Accession (Same CDS sequence)	XM_024595813.1 , XM_024595814.1
Accession Version	XM_024595813.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5979bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-04-08
Organism	Populus trichocarpa(black cottonwood)
Product	endoribonuclease Dicer homolog 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037286.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Populus trichocarpa Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGAGCG AGAGTAATGG TAGGGTTTCG GGGATTGGAG GTGGAGGTGG TCCGTCTTAC 
TGGCTTGATG CATGTGAGGA TATTTCTTGT GATATTATAG ATGATTTTGT TGATTTTGAT 
ACTTCAATTG TTCCAGAATT ATCTGTTGAT AATAATTCCA ATGTTAATAA TGATTTCTTT 
GGAGGGATTG ATCACATTCT TGATAGTATC AAGAATGGTA GTGGTCTTCC TCCTCTTCAT 
AATGCTAGTA CGACAGCTAA TGTTTCCAAT GGAAATCGGG ATTGCATTGT TGGTGATGGT 
TGGTTTATTA ATGTTGAAAA TGGAGTTTGC CATGGGAGCT CGGTTTCACA ATCAAATGGT 
GGTGATAAGG ACAATATTGA CAGAAAAGGG CAGGTGGAGA ATGGTGGTAA TGGGTTGAAT 
TTGTCCAATG GGAAGAGAGA AGAGAGGTTC TCAAACAATT TTGTTAAAGA AAATGGGAAG 
AAGGATGAGC AGTCGACGGA GCAAGGTATT GATGGTGATG AAAGGTGCGG TAAAAGGGCT 
AGACTTTGTT GTTATAGAAA TGAGAGAGTG TATTCTAGTA GAGGGGAGCA TAGGGATAGG 
GAGAGATGTT CTAGCAGGAA GAGGTCACGG GATTGGGATG AAAGCGATAG AAGGGATAGG 
GATATTAGTC GAAGAAGAGA TAGATATAGT GGTAGTAATA GGAGGGATGG TAGAGATAGG 
GATTGGAGGG AAAGAGAACT TAGGGGTTAT TGGGAGAGGG ATCGTTCAGG GTCCAAAGAC 
ATGGTTTTCC GATTGGGCAC TTGGGAAGCT GATCATAACA AAGAAGGAAG GGAGGCAAAT 
GACAAAATTC AGGAGTGCAA AGGAGAGCTA GAGAAGAAAT CAGAGGAGAG TAAAGAGAAA 
GTCCCTGAAG AACAGGCCCG CCAGTATCAA TTGGATGTTC TTGACCAGGC TAAGAAGAAA 
AATACAATAG CTTTTCTTGA AACAGGGGCA GGGAAGACAC TCATTGCTGT ACTCCTCATC 
AGAAGCATTT GCAATGACTT GCAAAGGCAG AATAAAAAAA TTCTAGCTGT ATTTTTAGTT 
CCGAAGGTTC CACTTGTTTA TCAGCAAGCA GAAGTTATTC GTGAGCGCGG TTACCAAGTG 
GGCCATTATT GTGGTGAGAT GGGTCAAGAT TTCTGGGATA CTCGAAGATG GCAGCGTGAG 
TTTGAAACAA AACAGGTTTT AGTAATGACA GCCCAAATTC TTTTGAACAT TCTGAGGCAC 
AGCATAATCA AAATGGAAGC CATAAATCTC CTCATTCTGG ATGAGTGTCA TCATGCTGTG 
AAGAAACATC CATATTCTCT TGTGATGTCT GAGTTTTACC ATACGACTCC CAAGGAGAAG 
AGACCGTCTG TTTTTGGAAT GACAGCTTCT CCTGTTAACT TAAAGGGTGT TTCAAGTCAG 
GTGGATTGTG CAATAAAGAT CCGCAATCTT GAAAGTAAAC TGGACTCAAT TGTTTGTACG 
ATAAAAGACC GCAAGGAACT GGAAAAGCAT GTACCAATGC CGGCAGAGGT TGTGGTGGAG 
TACGACAAAG CAGCTAGCTT GTGGTCCCTT CATGAACAAA TAAAGCAAAT CGAAGCAGCT 
GTTGAGGAAG CTGCACAGTC TAGCTCGAGA AGAAGTAAAT GGCAATTTAT GGGAGCTAGG 
GATGCTGGGG CCAAGGAAGA GCTGCGCCAG GTTTATGGTG TCTCTGAAAG AACAGAAAGT 
GATGGGGCTG CTAACTTAAT TCAAAAGTTG AGGGCAATTA ATTATGCACT TGGTGATCTG 
GGTCAGTGGT GTGCTTACAA GGTTGCACAA TCCTTTCTGA CTGCCTTACA AAATGACGAG 
AGAGCAAACT ATCAGCTTGA TGTTAAGTTT CAAGAATCCT ACCTCGAAAG AGTCGTGTTG 
CTCTTACAAT GCCAATTAAC AGAGGGAGCT GTTACAGACA AGGATACAAA AGTTTCAGAC 
AATGGGAATG ACAATATTCA GGATGGGCCT GGTTTTGATG AGATTGAGGA GGGAGAGCTC 
CCTGACAGTC ATGTTGTCTC TGGTGGAGAA CATGTTGATG TGATAATAGG AGCTGCTGTA 
GCTGATGGAA AAGTGACCCC TAAAGTGCAG TCACTGATTA AAGTACTTCT CAGATATCAG 
CACACGGAGG ATTTTCGTGC GATCATCTTT GTTGAGCGCG TAGTAGCTGC TTTGGTTCTT 
CCTAAGGTCT TTGCAGAGCT TCCATCTCTA AGTTTTGTTA GGTGTGCAAG TTTGATAGGG 
CACAATAATA GTCAGGAAAT GAGAACAAGC CAAATGCAAG ACACAATTGC CAAATTTCGA 
GATGGTCGTG TGACATTGTT AGTTGCTACC AGTGTTGCTG AGGAAGGACT TGATATTAGG 
CAATGCAATG TTGTTATTCG CTTTGATCTT GCAAAAACTG TTCTTGCATA TATTCAGTCT 
CGAGGTCGTG CAAGAAAGCC AGGCTCTGAT TACATCTTGA TGGTGGAGAG GGGAAACCTG 
TCTCATGGAG CATTCTTAAG GAATGCTAGG AATAGTGAGG AGACCCTGAG GAAAGAAGCG 
ATTGAGAGAA CTGACCTCAG TCATCTTAAG GATACTTCAA GGTTAATTGC AGTAGATTCA 
ATACCAGGAA CTGTGTATCA GGTGGAGTCT ACTGGTGCTG TTGTGAGCTT AAATTCTGCT 
GTTGGACTTG TTCATTTCTA TTGCTCTCAG TTACCCAGTG ACAGGTATTC AATACTTCGT 
CCTGGGTTTA TTATGGAGAA GCATGAGAAG CCAGGAGGTC CCACGGAATA TTCATGCAAG 
CTTCAGCTTC CTTGTAATGC ACCATTTGAG GAGCTTGAGG GTCCTGTATG CAGTTCAATG 
CGCCTTGCAC ATCAGGCTGT TTGTTTGGCT GCTTGCAAGA AGCTTCATGA GATGGGAGCA 
TTTACTGACA TGCTCTTGCC AGACAAGGGA AGTGAGGAAG AAAAGGACAA GGTTGACCAG 
AATGATGAAG GTGAACCACT TCCTGGTACT GCTAGGCATC GGGAGTTCTA TCCTGAAGGT 
GTAGCTAAGA CACTCCAGGG AGAATGGATC TTATGCGGAA GGGATGGTTG CAACAACTCC 
AAAGTGCTTC ATCTCTACTT GTATGGAGTC AGATGCTTAA ACATTGGCAC TTCAAATGAT 
CCATTCTTGA CTCAAGTTTC AAATTTTGCA GTGCTTTTTG GCAATGAGCT GGATGCAGAG 
GTATATCAAT TTATTTTCAG TTTCAGCATA GATAGATATT TTAAGCATTT TTCTTTAACA 
CCTCAATTTC TTCAGGTCTT ATCGATGTCG ATGGATCTAT TTATCGCTCG AACCATGATA 
ACGAAGGCAT CTCTTGTCTT TAGGGGTCGC ATACCTATTA CAGAAAGTCA GCTGGCATCC 
CTTAAAAATT TTCATGTAAG GTTGATGAGC ATTGTACTGG ATGTGGATGT TGAACCTTCC 
ACCACTCCAT GGGATCCTGC AAAAGCTTAT TTATTCGTCC CTATGGTTAG CGATAAGTCT 
GTAGATCCTA TAAAAGAAAT TGACTGGGAT CTGGTTGAAA ATATAATTGG AACAGATGCT 
TGGAGCAATC GTCTTCAGAG AGCTCGACCA GATGTTTACC TTGGTACAAA TGAGAGGACA 
CTTGGTGGAG ACAGGAGGGA GTATGGTTTT GGGAAATTGC GACATGGAAT TGCTTTTGGA 
CAGAAACCTC ATCCCACTTA TGGCATCAGG GGAGCTGTAG CACAGTTTGA TGTTGTGAAA 
GCTTCTGGAT TGATTCCTAA GAGGGGATGG GATGCAACTG AGACGCAAAA ACTGGAGCTG 
ACCAAAGGCA AATTGATGAT GGCTGATACT TGTGTCAATG CTGATGCTTT GATGGGGAGA 
ATTGTAACAG CTGCTCACTC TGGGAAGAGG TTTTATGTGG ATTCCATATG CTATGATATG 
ACTGCAGAGA TCTCATTTCC AAGAAAAGAG GGTTATCTTG GTCCTCTGGA GTACAGCTCA 
TATGCTGATT ACTACAAGCA AAAGTATGGA GTTGAATTGA AATTTAAGCA GCAGCCTTTA 
CTAAGAGGAC GTGGTGTTTC ATACTGCAAG AATCTCTTAT CTCCTCGGTT TGAACACTCA 
GATTCAAATG AAGGCGATGC TGAAGAGAAT CTTGACAAGA CTTACTATGT GTTTCTTCCT 
CCTGAGCTGT GTCTGGTGCA CCCTCTTCCT GGATCACTTG TTCGAGGTGC CCAGAGATTA 
CCATCGATAA TGAGGAGGGT TGAAAGCATG CTGCTCGCTG TTGAACTTAA GGATATAATA 
AATTATCCAG TCCCTGCTTC AAAGATCTTG GAAGCCTTGA CTGCTGCTTC ATGCCAAGAG 
ACATTCTGCT ATGAAAGAGC AGAGCTTCTA GGAGATGCTT ACCTGAAATG GGTGGTTAGT 
CGATTTCTTT TTCTGAAGTA TCCCCAGAAA CATGAGGGTC AACTTACTAG AATGAGGCAA 
CAAATGGTAA GTAATATGGT TTTGTATCAA TATGCACTTA ACAAAGGACT TCAGTCATAT 
ATCCAAGCAG ATCGCTTTGC TCCATCTCGA TGGGCTGCTC CTGGAGTGTT GCCTGTCTTT 
GATGAGGAAA CAAAAGATGG AGACTCGTAC ATATTTGATC AGGAGAAGTC ACTTGCTGAG 
GATAGAACCG GAATGAATCA TCTTGATGAT GGGTATGAAA ATGAAATTGA AGATGGTGAA 
CTTGAGAGTG ATGCTAGTTC TTACAGAGTC CTCTCTAGCA AGACATTGGC AGATGTTGTT 
GAAGCATTAA TTGGTGTTTA TTATGTTGAA GGTGGGAAAA ATGCTGTTAA CCACCTTATG 
AAATGGATTG GAATTCAGGT AGAGTTTGAT CATGAAGAGA TAGATGGTGC ATCTAGGCCA 
TTCAATGTTC CAGAAAGTGT TCTTAGGAGT GTTGACTTTG ATACTTTAGA AGGTGCCTTG 
GATATTAAGT TCAATGATCG TGGCTTGTTG ATAGAAGCCA TAACGCATGC TTCACGACCA 
TCTTCTGGAG TATCCTGCTA TCAACGATTA GAATTTGTTG GTGACGCAGT CTTAGATCAT 
CTTATTACTA GGCACTTGTT TTTTACATAT ACAAATCTGC CCCCTGGTCG CTTGACAGAC 
TTGCGAGCCG CTGCTGTAAA CAATGAAAAT TTTGCACGTG TTGCAGTTAA ACATAAGCTT 
CATGTGCACC TTAGACATGG GTCAAGTGCC CTTGAAAAGC AGATTCGGGA CTTTGTTAGG 
GAAGTTCAAG ATGAACTATT GAAGCCAGTG TTCAACTCCT TTGGTTTGGG AGATTGCAAA 
GCTCCAAAGG TTCTTGGTGA CATCGTTGAA TCCATTGCTG GTGCTATTTT TCTTGACAGC 
GGACGAGATA CAGCGGTTGT GTGGAAGGTT TTTCAACCTC TGTTGCATCC AATGGTCACT 
CCAGAAACCC TGCCAATGCA TCCTGTAAGG GAGCTGCAGG AACGGTGCCA GCAACAGGCT 
GAAGGATTGG AATACAAAGC CACTCGAAGT GGCAATTTGG CTACTGTTGA AGTTTTCATT 
GATGGTGTTC AGGTAGGAGT TGCCCAAAAT CCACAAAAGA AAATGGCACA GAAACTCGCT 
GCTAGGAATG CACTTGTTGT TTTGAAAGAG AAGGAAACAG CTGAAGCCAA GGAAAAGAGT 
GATGAAAATG GGAAGAAGAA AAGGAACGGC AACCAAACCT TTACTAGACA AACATTAAAT 
GATATCTGCT TGCGTAGAAA TTGGCCTATG CCATCATATC GGTGTGTCAA TGAGGGTGGT 
CCTGCACATG CAAAGAGGTT TACTTTTGCA GTTCGTGTGA ATACTACCGA TAGAGGATGG 
ACAGACGAAT GTGTGGGAGA GCCGATGCCG AGTGTTAAGA AGGCCAAAGA CTCTGCTGCA 
GTTCTTCTCT TGGAACTATT AAATAAACGG TACTCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024451581.1
CDS	924..6902
Translation	MESESNGRVSGIGGGGGPSYWLDACEDISCDIIDDFVDFDTSIVPELSVDNNSNVNNDFFGGIDHILDSIKNGSGLPPLHNASTTANVSNGNRDCIVGDGWFINVENGVCHGSSVSQSNGGDKDNIDRKGQVENGGNGLNLSNGKREERFSNNFVKENGKKDEQSTEQGIDGDERCGKRARLCCYRNERVYSSRGEHRDRERCSSRKRSRDWDESDRRDRDISRRRDRYSGSNRRDGRDRDWRERELRGYWERDRSGSKDMVFRLGTWEADHNKEGREANDKIQECKGELEKKSEESKEKVPEEQARQYQLDVLDQAKKKNTIAFLETGAGKTLIAVLLIRSICNDLQRQNKKILAVFLVPKVPLVYQQAEVIRERGYQVGHYCGEMGQDFWDTRRWQREFETKQVLVMTAQILLNILRHSIIKMEAINLLILDECHHAVKKHPYSLVMSEFYHTTPKEKRPSVFGMTASPVNLKGVSSQVDCAIKIRNLESKLDSIVCTIKDRKELEKHVPMPAEVVVEYDKAASLWSLHEQIKQIEAAVEEAAQSSSRRSKWQFMGARDAGAKEELRQVYGVSERTESDGAANLIQKLRAINYALGDLGQWCAYKVAQSFLTALQNDERANYQLDVKFQESYLERVVLLLQCQLTEGAVTDKDTKVSDNGNDNIQDGPGFDEIEEGELPDSHVVSGGEHVDVIIGAAVADGKVTPKVQSLIKVLLRYQHTEDFRAIIFVERVVAALVLPKVFAELPSLSFVRCASLIGHNNSQEMRTSQMQDTIAKFRDGRVTLLVATSVAEEGLDIRQCNVVIRFDLAKTVLAYIQSRGRARKPGSDYILMVERGNLSHGAFLRNARNSEETLRKEAIERTDLSHLKDTSRLIAVDSIPGTVYQVESTGAVVSLNSAVGLVHFYCSQLPSDRYSILRPGFIMEKHEKPGGPTEYSCKLQLPCNAPFEELEGPVCSSMRLAHQAVCLAACKKLHEMGAFTDMLLPDKGSEEEKDKVDQNDEGEPLPGTARHREFYPEGVAKTLQGEWILCGRDGCNNSKVLHLYLYGVRCLNIGTSNDPFLTQVSNFAVLFGNELDAEVYQFIFSFSIDRYFKHFSLTPQFLQVLSMSMDLFIARTMITKASLVFRGRIPITESQLASLKNFHVRLMSIVLDVDVEPSTTPWDPAKAYLFVPMVSDKSVDPIKEIDWDLVENIIGTDAWSNRLQRARPDVYLGTNERTLGGDRREYGFGKLRHGIAFGQKPHPTYGIRGAVAQFDVVKASGLIPKRGWDATETQKLELTKGKLMMADTCVNADALMGRIVTAAHSGKRFYVDSICYDMTAEISFPRKEGYLGPLEYSSYADYYKQKYGVELKFKQQPLLRGRGVSYCKNLLSPRFEHSDSNEGDAEENLDKTYYVFLPPELCLVHPLPGSLVRGAQRLPSIMRRVESMLLAVELKDIINYPVPASKILEALTAASCQETFCYERAELLGDAYLKWVVSRFLFLKYPQKHEGQLTRMRQQMVSNMVLYQYALNKGLQSYIQADRFAPSRWAAPGVLPVFDEETKDGDSYIFDQEKSLAEDRTGMNHLDDGYENEIEDGELESDASSYRVLSSKTLADVVEALIGVYYVEGGKNAVNHLMKWIGIQVEFDHEEIDGASRPFNVPESVLRSVDFDTLEGALDIKFNDRGLLIEAITHASRPSSGVSCYQRLEFVGDAVLDHLITRHLFFTYTNLPPGRLTDLRAAAVNNENFARVAVKHKLHVHLRHGSSALEKQIRDFVREVQDELLKPVFNSFGLGDCKAPKVLGDIVESIAGAIFLDSGRDTAVVWKVFQPLLHPMVTPETLPMHPVRELQERCQQQAEGLEYKATRSGNLATVEVFIDGVQVGVAQNPQKKMAQKLAARNALVVLKEKETAEAKEKSDENGKKKRNGNQTFTRQTLNDICLRRNWPMPSYRCVNEGGPAHAKRFTFAVRVNTTDRGWTDECVGEPMPSVKKAKDSAAVLLLELLNKRYS

Target ORF information:

RefSeq Version	XM_024595813.1
Organism	Populus trichocarpa(black cottonwood)
Definition	Populus trichocarpa endoribonuclease Dicer homolog 1 (LOC18096402), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024595813.1

ORF Insert Sequence:

ATGGAGAGCG AGAGTAATGG TAGGGTTTCG GGGATTGGAG GTGGAGGTGG TCCGTCTTAC 
TGGCTTGATG CATGTGAGGA TATTTCTTGT GATATTATAG ATGATTTTGT TGATTTTGAT 
ACTTCAATTG TTCCAGAATT ATCTGTTGAT AATAATTCCA ATGTTAATAA TGATTTCTTT 
GGAGGGATTG ATCACATTCT TGATAGTATC AAGAATGGTA GTGGTCTTCC TCCTCTTCAT 
AATGCTAGTA CGACAGCTAA TGTTTCCAAT GGAAATCGGG ATTGCATTGT TGGTGATGGT 
TGGTTTATTA ATGTTGAAAA TGGAGTTTGC CATGGGAGCT CGGTTTCACA ATCAAATGGT 
GGTGATAAGG ACAATATTGA CAGAAAAGGG CAGGTGGAGA ATGGTGGTAA TGGGTTGAAT 
TTGTCCAATG GGAAGAGAGA AGAGAGGTTC TCAAACAATT TTGTTAAAGA AAATGGGAAG 
AAGGATGAGC AGTCGACGGA GCAAGGTATT GATGGTGATG AAAGGTGCGG TAAAAGGGCT 
AGACTTTGTT GTTATAGAAA TGAGAGAGTG TATTCTAGTA GAGGGGAGCA TAGGGATAGG 
GAGAGATGTT CTAGCAGGAA GAGGTCACGG GATTGGGATG AAAGCGATAG AAGGGATAGG 
GATATTAGTC GAAGAAGAGA TAGATATAGT GGTAGTAATA GGAGGGATGG TAGAGATAGG 
GATTGGAGGG AAAGAGAACT TAGGGGTTAT TGGGAGAGGG ATCGTTCAGG GTCCAAAGAC 
ATGGTTTTCC GATTGGGCAC TTGGGAAGCT GATCATAACA AAGAAGGAAG GGAGGCAAAT 
GACAAAATTC AGGAGTGCAA AGGAGAGCTA GAGAAGAAAT CAGAGGAGAG TAAAGAGAAA 
GTCCCTGAAG AACAGGCCCG CCAGTATCAA TTGGATGTTC TTGACCAGGC TAAGAAGAAA 
AATACAATAG CTTTTCTTGA AACAGGGGCA GGGAAGACAC TCATTGCTGT ACTCCTCATC 
AGAAGCATTT GCAATGACTT GCAAAGGCAG AATAAAAAAA TTCTAGCTGT ATTTTTAGTT 
CCGAAGGTTC CACTTGTTTA TCAGCAAGCA GAAGTTATTC GTGAGCGCGG TTACCAAGTG 
GGCCATTATT GTGGTGAGAT GGGTCAAGAT TTCTGGGATA CTCGAAGATG GCAGCGTGAG 
TTTGAAACAA AACAGGTTTT AGTAATGACA GCCCAAATTC TTTTGAACAT TCTGAGGCAC 
AGCATAATCA AAATGGAAGC CATAAATCTC CTCATTCTGG ATGAGTGTCA TCATGCTGTG 
AAGAAACATC CATATTCTCT TGTGATGTCT GAGTTTTACC ATACGACTCC CAAGGAGAAG 
AGACCGTCTG TTTTTGGAAT GACAGCTTCT CCTGTTAACT TAAAGGGTGT TTCAAGTCAG 
GTGGATTGTG CAATAAAGAT CCGCAATCTT GAAAGTAAAC TGGACTCAAT TGTTTGTACG 
ATAAAAGACC GCAAGGAACT GGAAAAGCAT GTACCAATGC CGGCAGAGGT TGTGGTGGAG 
TACGACAAAG CAGCTAGCTT GTGGTCCCTT CATGAACAAA TAAAGCAAAT CGAAGCAGCT 
GTTGAGGAAG CTGCACAGTC TAGCTCGAGA AGAAGTAAAT GGCAATTTAT GGGAGCTAGG 
GATGCTGGGG CCAAGGAAGA GCTGCGCCAG GTTTATGGTG TCTCTGAAAG AACAGAAAGT 
GATGGGGCTG CTAACTTAAT TCAAAAGTTG AGGGCAATTA ATTATGCACT TGGTGATCTG 
GGTCAGTGGT GTGCTTACAA GGTTGCACAA TCCTTTCTGA CTGCCTTACA AAATGACGAG 
AGAGCAAACT ATCAGCTTGA TGTTAAGTTT CAAGAATCCT ACCTCGAAAG AGTCGTGTTG 
CTCTTACAAT GCCAATTAAC AGAGGGAGCT GTTACAGACA AGGATACAAA AGTTTCAGAC 
AATGGGAATG ACAATATTCA GGATGGGCCT GGTTTTGATG AGATTGAGGA GGGAGAGCTC 
CCTGACAGTC ATGTTGTCTC TGGTGGAGAA CATGTTGATG TGATAATAGG AGCTGCTGTA 
GCTGATGGAA AAGTGACCCC TAAAGTGCAG TCACTGATTA AAGTACTTCT CAGATATCAG 
CACACGGAGG ATTTTCGTGC GATCATCTTT GTTGAGCGCG TAGTAGCTGC TTTGGTTCTT 
CCTAAGGTCT TTGCAGAGCT TCCATCTCTA AGTTTTGTTA GGTGTGCAAG TTTGATAGGG 
CACAATAATA GTCAGGAAAT GAGAACAAGC CAAATGCAAG ACACAATTGC CAAATTTCGA 
GATGGTCGTG TGACATTGTT AGTTGCTACC AGTGTTGCTG AGGAAGGACT TGATATTAGG 
CAATGCAATG TTGTTATTCG CTTTGATCTT GCAAAAACTG TTCTTGCATA TATTCAGTCT 
CGAGGTCGTG CAAGAAAGCC AGGCTCTGAT TACATCTTGA TGGTGGAGAG GGGAAACCTG 
TCTCATGGAG CATTCTTAAG GAATGCTAGG AATAGTGAGG AGACCCTGAG GAAAGAAGCG 
ATTGAGAGAA CTGACCTCAG TCATCTTAAG GATACTTCAA GGTTAATTGC AGTAGATTCA 
ATACCAGGAA CTGTGTATCA GGTGGAGTCT ACTGGTGCTG TTGTGAGCTT AAATTCTGCT 
GTTGGACTTG TTCATTTCTA TTGCTCTCAG TTACCCAGTG ACAGGTATTC AATACTTCGT 
CCTGGGTTTA TTATGGAGAA GCATGAGAAG CCAGGAGGTC CCACGGAATA TTCATGCAAG 
CTTCAGCTTC CTTGTAATGC ACCATTTGAG GAGCTTGAGG GTCCTGTATG CAGTTCAATG 
CGCCTTGCAC ATCAGGCTGT TTGTTTGGCT GCTTGCAAGA AGCTTCATGA GATGGGAGCA 
TTTACTGACA TGCTCTTGCC AGACAAGGGA AGTGAGGAAG AAAAGGACAA GGTTGACCAG 
AATGATGAAG GTGAACCACT TCCTGGTACT GCTAGGCATC GGGAGTTCTA TCCTGAAGGT 
GTAGCTAAGA CACTCCAGGG AGAATGGATC TTATGCGGAA GGGATGGTTG CAACAACTCC 
AAAGTGCTTC ATCTCTACTT GTATGGAGTC AGATGCTTAA ACATTGGCAC TTCAAATGAT 
CCATTCTTGA CTCAAGTTTC AAATTTTGCA GTGCTTTTTG GCAATGAGCT GGATGCAGAG 
GTATATCAAT TTATTTTCAG TTTCAGCATA GATAGATATT TTAAGCATTT TTCTTTAACA 
CCTCAATTTC TTCAGGTCTT ATCGATGTCG ATGGATCTAT TTATCGCTCG AACCATGATA 
ACGAAGGCAT CTCTTGTCTT TAGGGGTCGC ATACCTATTA CAGAAAGTCA GCTGGCATCC 
CTTAAAAATT TTCATGTAAG GTTGATGAGC ATTGTACTGG ATGTGGATGT TGAACCTTCC 
ACCACTCCAT GGGATCCTGC AAAAGCTTAT TTATTCGTCC CTATGGTTAG CGATAAGTCT 
GTAGATCCTA TAAAAGAAAT TGACTGGGAT CTGGTTGAAA ATATAATTGG AACAGATGCT 
TGGAGCAATC GTCTTCAGAG AGCTCGACCA GATGTTTACC TTGGTACAAA TGAGAGGACA 
CTTGGTGGAG ACAGGAGGGA GTATGGTTTT GGGAAATTGC GACATGGAAT TGCTTTTGGA 
CAGAAACCTC ATCCCACTTA TGGCATCAGG GGAGCTGTAG CACAGTTTGA TGTTGTGAAA 
GCTTCTGGAT TGATTCCTAA GAGGGGATGG GATGCAACTG AGACGCAAAA ACTGGAGCTG 
ACCAAAGGCA AATTGATGAT GGCTGATACT TGTGTCAATG CTGATGCTTT GATGGGGAGA 
ATTGTAACAG CTGCTCACTC TGGGAAGAGG TTTTATGTGG ATTCCATATG CTATGATATG 
ACTGCAGAGA TCTCATTTCC AAGAAAAGAG GGTTATCTTG GTCCTCTGGA GTACAGCTCA 
TATGCTGATT ACTACAAGCA AAAGTATGGA GTTGAATTGA AATTTAAGCA GCAGCCTTTA 
CTAAGAGGAC GTGGTGTTTC ATACTGCAAG AATCTCTTAT CTCCTCGGTT TGAACACTCA 
GATTCAAATG AAGGCGATGC TGAAGAGAAT CTTGACAAGA CTTACTATGT GTTTCTTCCT 
CCTGAGCTGT GTCTGGTGCA CCCTCTTCCT GGATCACTTG TTCGAGGTGC CCAGAGATTA 
CCATCGATAA TGAGGAGGGT TGAAAGCATG CTGCTCGCTG TTGAACTTAA GGATATAATA 
AATTATCCAG TCCCTGCTTC AAAGATCTTG GAAGCCTTGA CTGCTGCTTC ATGCCAAGAG 
ACATTCTGCT ATGAAAGAGC AGAGCTTCTA GGAGATGCTT ACCTGAAATG GGTGGTTAGT 
CGATTTCTTT TTCTGAAGTA TCCCCAGAAA CATGAGGGTC AACTTACTAG AATGAGGCAA 
CAAATGGTAA GTAATATGGT TTTGTATCAA TATGCACTTA ACAAAGGACT TCAGTCATAT 
ATCCAAGCAG ATCGCTTTGC TCCATCTCGA TGGGCTGCTC CTGGAGTGTT GCCTGTCTTT 
GATGAGGAAA CAAAAGATGG AGACTCGTAC ATATTTGATC AGGAGAAGTC ACTTGCTGAG 
GATAGAACCG GAATGAATCA TCTTGATGAT GGGTATGAAA ATGAAATTGA AGATGGTGAA 
CTTGAGAGTG ATGCTAGTTC TTACAGAGTC CTCTCTAGCA AGACATTGGC AGATGTTGTT 
GAAGCATTAA TTGGTGTTTA TTATGTTGAA GGTGGGAAAA ATGCTGTTAA CCACCTTATG 
AAATGGATTG GAATTCAGGT AGAGTTTGAT CATGAAGAGA TAGATGGTGC ATCTAGGCCA 
TTCAATGTTC CAGAAAGTGT TCTTAGGAGT GTTGACTTTG ATACTTTAGA AGGTGCCTTG 
GATATTAAGT TCAATGATCG TGGCTTGTTG ATAGAAGCCA TAACGCATGC TTCACGACCA 
TCTTCTGGAG TATCCTGCTA TCAACGATTA GAATTTGTTG GTGACGCAGT CTTAGATCAT 
CTTATTACTA GGCACTTGTT TTTTACATAT ACAAATCTGC CCCCTGGTCG CTTGACAGAC 
TTGCGAGCCG CTGCTGTAAA CAATGAAAAT TTTGCACGTG TTGCAGTTAA ACATAAGCTT 
CATGTGCACC TTAGACATGG GTCAAGTGCC CTTGAAAAGC AGATTCGGGA CTTTGTTAGG 
GAAGTTCAAG ATGAACTATT GAAGCCAGTG TTCAACTCCT TTGGTTTGGG AGATTGCAAA 
GCTCCAAAGG TTCTTGGTGA CATCGTTGAA TCCATTGCTG GTGCTATTTT TCTTGACAGC 
GGACGAGATA CAGCGGTTGT GTGGAAGGTT TTTCAACCTC TGTTGCATCC AATGGTCACT 
CCAGAAACCC TGCCAATGCA TCCTGTAAGG GAGCTGCAGG AACGGTGCCA GCAACAGGCT 
GAAGGATTGG AATACAAAGC CACTCGAAGT GGCAATTTGG CTACTGTTGA AGTTTTCATT 
GATGGTGTTC AGGTAGGAGT TGCCCAAAAT CCACAAAAGA AAATGGCACA GAAACTCGCT 
GCTAGGAATG CACTTGTTGT TTTGAAAGAG AAGGAAACAG CTGAAGCCAA GGAAAAGAGT 
GATGAAAATG GGAAGAAGAA AAGGAACGGC AACCAAACCT TTACTAGACA AACATTAAAT 
GATATCTGCT TGCGTAGAAA TTGGCCTATG CCATCATATC GGTGTGTCAA TGAGGGTGGT 
CCTGCACATG CAAAGAGGTT TACTTTTGCA GTTCGTGTGA ATACTACCGA TAGAGGATGG 
ACAGACGAAT GTGTGGGAGA GCCGATGCCG AGTGTTAAGA AGGCCAAAGA CTCTGCTGCA 
GTTCTTCTCT TGGAACTATT AAATAAACGG TACTCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC18096402 ( XM_024595814.1 ) cDNA ORF clone, Populus trichocarpa(black cottonwood) -> XP_024451582.1 Populus trichocarpa endoribonuclease Dicer homolog 1 (LOC18096402), transcript variant X2, mRNA.

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CloneID	OPa425075
Clone ID Related Accession (Same CDS sequence)	XM_024595813.1 , XM_024595814.1
Accession Version	XM_024595814.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5979bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-04-08
Organism	Populus trichocarpa(black cottonwood)
Product	endoribonuclease Dicer homolog 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037286.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Populus trichocarpa Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGAGCG AGAGTAATGG TAGGGTTTCG GGGATTGGAG GTGGAGGTGG TCCGTCTTAC 
TGGCTTGATG CATGTGAGGA TATTTCTTGT GATATTATAG ATGATTTTGT TGATTTTGAT 
ACTTCAATTG TTCCAGAATT ATCTGTTGAT AATAATTCCA ATGTTAATAA TGATTTCTTT 
GGAGGGATTG ATCACATTCT TGATAGTATC AAGAATGGTA GTGGTCTTCC TCCTCTTCAT 
AATGCTAGTA CGACAGCTAA TGTTTCCAAT GGAAATCGGG ATTGCATTGT TGGTGATGGT 
TGGTTTATTA ATGTTGAAAA TGGAGTTTGC CATGGGAGCT CGGTTTCACA ATCAAATGGT 
GGTGATAAGG ACAATATTGA CAGAAAAGGG CAGGTGGAGA ATGGTGGTAA TGGGTTGAAT 
TTGTCCAATG GGAAGAGAGA AGAGAGGTTC TCAAACAATT TTGTTAAAGA AAATGGGAAG 
AAGGATGAGC AGTCGACGGA GCAAGGTATT GATGGTGATG AAAGGTGCGG TAAAAGGGCT 
AGACTTTGTT GTTATAGAAA TGAGAGAGTG TATTCTAGTA GAGGGGAGCA TAGGGATAGG 
GAGAGATGTT CTAGCAGGAA GAGGTCACGG GATTGGGATG AAAGCGATAG AAGGGATAGG 
GATATTAGTC GAAGAAGAGA TAGATATAGT GGTAGTAATA GGAGGGATGG TAGAGATAGG 
GATTGGAGGG AAAGAGAACT TAGGGGTTAT TGGGAGAGGG ATCGTTCAGG GTCCAAAGAC 
ATGGTTTTCC GATTGGGCAC TTGGGAAGCT GATCATAACA AAGAAGGAAG GGAGGCAAAT 
GACAAAATTC AGGAGTGCAA AGGAGAGCTA GAGAAGAAAT CAGAGGAGAG TAAAGAGAAA 
GTCCCTGAAG AACAGGCCCG CCAGTATCAA TTGGATGTTC TTGACCAGGC TAAGAAGAAA 
AATACAATAG CTTTTCTTGA AACAGGGGCA GGGAAGACAC TCATTGCTGT ACTCCTCATC 
AGAAGCATTT GCAATGACTT GCAAAGGCAG AATAAAAAAA TTCTAGCTGT ATTTTTAGTT 
CCGAAGGTTC CACTTGTTTA TCAGCAAGCA GAAGTTATTC GTGAGCGCGG TTACCAAGTG 
GGCCATTATT GTGGTGAGAT GGGTCAAGAT TTCTGGGATA CTCGAAGATG GCAGCGTGAG 
TTTGAAACAA AACAGGTTTT AGTAATGACA GCCCAAATTC TTTTGAACAT TCTGAGGCAC 
AGCATAATCA AAATGGAAGC CATAAATCTC CTCATTCTGG ATGAGTGTCA TCATGCTGTG 
AAGAAACATC CATATTCTCT TGTGATGTCT GAGTTTTACC ATACGACTCC CAAGGAGAAG 
AGACCGTCTG TTTTTGGAAT GACAGCTTCT CCTGTTAACT TAAAGGGTGT TTCAAGTCAG 
GTGGATTGTG CAATAAAGAT CCGCAATCTT GAAAGTAAAC TGGACTCAAT TGTTTGTACG 
ATAAAAGACC GCAAGGAACT GGAAAAGCAT GTACCAATGC CGGCAGAGGT TGTGGTGGAG 
TACGACAAAG CAGCTAGCTT GTGGTCCCTT CATGAACAAA TAAAGCAAAT CGAAGCAGCT 
GTTGAGGAAG CTGCACAGTC TAGCTCGAGA AGAAGTAAAT GGCAATTTAT GGGAGCTAGG 
GATGCTGGGG CCAAGGAAGA GCTGCGCCAG GTTTATGGTG TCTCTGAAAG AACAGAAAGT 
GATGGGGCTG CTAACTTAAT TCAAAAGTTG AGGGCAATTA ATTATGCACT TGGTGATCTG 
GGTCAGTGGT GTGCTTACAA GGTTGCACAA TCCTTTCTGA CTGCCTTACA AAATGACGAG 
AGAGCAAACT ATCAGCTTGA TGTTAAGTTT CAAGAATCCT ACCTCGAAAG AGTCGTGTTG 
CTCTTACAAT GCCAATTAAC AGAGGGAGCT GTTACAGACA AGGATACAAA AGTTTCAGAC 
AATGGGAATG ACAATATTCA GGATGGGCCT GGTTTTGATG AGATTGAGGA GGGAGAGCTC 
CCTGACAGTC ATGTTGTCTC TGGTGGAGAA CATGTTGATG TGATAATAGG AGCTGCTGTA 
GCTGATGGAA AAGTGACCCC TAAAGTGCAG TCACTGATTA AAGTACTTCT CAGATATCAG 
CACACGGAGG ATTTTCGTGC GATCATCTTT GTTGAGCGCG TAGTAGCTGC TTTGGTTCTT 
CCTAAGGTCT TTGCAGAGCT TCCATCTCTA AGTTTTGTTA GGTGTGCAAG TTTGATAGGG 
CACAATAATA GTCAGGAAAT GAGAACAAGC CAAATGCAAG ACACAATTGC CAAATTTCGA 
GATGGTCGTG TGACATTGTT AGTTGCTACC AGTGTTGCTG AGGAAGGACT TGATATTAGG 
CAATGCAATG TTGTTATTCG CTTTGATCTT GCAAAAACTG TTCTTGCATA TATTCAGTCT 
CGAGGTCGTG CAAGAAAGCC AGGCTCTGAT TACATCTTGA TGGTGGAGAG GGGAAACCTG 
TCTCATGGAG CATTCTTAAG GAATGCTAGG AATAGTGAGG AGACCCTGAG GAAAGAAGCG 
ATTGAGAGAA CTGACCTCAG TCATCTTAAG GATACTTCAA GGTTAATTGC AGTAGATTCA 
ATACCAGGAA CTGTGTATCA GGTGGAGTCT ACTGGTGCTG TTGTGAGCTT AAATTCTGCT 
GTTGGACTTG TTCATTTCTA TTGCTCTCAG TTACCCAGTG ACAGGTATTC AATACTTCGT 
CCTGGGTTTA TTATGGAGAA GCATGAGAAG CCAGGAGGTC CCACGGAATA TTCATGCAAG 
CTTCAGCTTC CTTGTAATGC ACCATTTGAG GAGCTTGAGG GTCCTGTATG CAGTTCAATG 
CGCCTTGCAC ATCAGGCTGT TTGTTTGGCT GCTTGCAAGA AGCTTCATGA GATGGGAGCA 
TTTACTGACA TGCTCTTGCC AGACAAGGGA AGTGAGGAAG AAAAGGACAA GGTTGACCAG 
AATGATGAAG GTGAACCACT TCCTGGTACT GCTAGGCATC GGGAGTTCTA TCCTGAAGGT 
GTAGCTAAGA CACTCCAGGG AGAATGGATC TTATGCGGAA GGGATGGTTG CAACAACTCC 
AAAGTGCTTC ATCTCTACTT GTATGGAGTC AGATGCTTAA ACATTGGCAC TTCAAATGAT 
CCATTCTTGA CTCAAGTTTC AAATTTTGCA GTGCTTTTTG GCAATGAGCT GGATGCAGAG 
GTATATCAAT TTATTTTCAG TTTCAGCATA GATAGATATT TTAAGCATTT TTCTTTAACA 
CCTCAATTTC TTCAGGTCTT ATCGATGTCG ATGGATCTAT TTATCGCTCG AACCATGATA 
ACGAAGGCAT CTCTTGTCTT TAGGGGTCGC ATACCTATTA CAGAAAGTCA GCTGGCATCC 
CTTAAAAATT TTCATGTAAG GTTGATGAGC ATTGTACTGG ATGTGGATGT TGAACCTTCC 
ACCACTCCAT GGGATCCTGC AAAAGCTTAT TTATTCGTCC CTATGGTTAG CGATAAGTCT 
GTAGATCCTA TAAAAGAAAT TGACTGGGAT CTGGTTGAAA ATATAATTGG AACAGATGCT 
TGGAGCAATC GTCTTCAGAG AGCTCGACCA GATGTTTACC TTGGTACAAA TGAGAGGACA 
CTTGGTGGAG ACAGGAGGGA GTATGGTTTT GGGAAATTGC GACATGGAAT TGCTTTTGGA 
CAGAAACCTC ATCCCACTTA TGGCATCAGG GGAGCTGTAG CACAGTTTGA TGTTGTGAAA 
GCTTCTGGAT TGATTCCTAA GAGGGGATGG GATGCAACTG AGACGCAAAA ACTGGAGCTG 
ACCAAAGGCA AATTGATGAT GGCTGATACT TGTGTCAATG CTGATGCTTT GATGGGGAGA 
ATTGTAACAG CTGCTCACTC TGGGAAGAGG TTTTATGTGG ATTCCATATG CTATGATATG 
ACTGCAGAGA TCTCATTTCC AAGAAAAGAG GGTTATCTTG GTCCTCTGGA GTACAGCTCA 
TATGCTGATT ACTACAAGCA AAAGTATGGA GTTGAATTGA AATTTAAGCA GCAGCCTTTA 
CTAAGAGGAC GTGGTGTTTC ATACTGCAAG AATCTCTTAT CTCCTCGGTT TGAACACTCA 
GATTCAAATG AAGGCGATGC TGAAGAGAAT CTTGACAAGA CTTACTATGT GTTTCTTCCT 
CCTGAGCTGT GTCTGGTGCA CCCTCTTCCT GGATCACTTG TTCGAGGTGC CCAGAGATTA 
CCATCGATAA TGAGGAGGGT TGAAAGCATG CTGCTCGCTG TTGAACTTAA GGATATAATA 
AATTATCCAG TCCCTGCTTC AAAGATCTTG GAAGCCTTGA CTGCTGCTTC ATGCCAAGAG 
ACATTCTGCT ATGAAAGAGC AGAGCTTCTA GGAGATGCTT ACCTGAAATG GGTGGTTAGT 
CGATTTCTTT TTCTGAAGTA TCCCCAGAAA CATGAGGGTC AACTTACTAG AATGAGGCAA 
CAAATGGTAA GTAATATGGT TTTGTATCAA TATGCACTTA ACAAAGGACT TCAGTCATAT 
ATCCAAGCAG ATCGCTTTGC TCCATCTCGA TGGGCTGCTC CTGGAGTGTT GCCTGTCTTT 
GATGAGGAAA CAAAAGATGG AGACTCGTAC ATATTTGATC AGGAGAAGTC ACTTGCTGAG 
GATAGAACCG GAATGAATCA TCTTGATGAT GGGTATGAAA ATGAAATTGA AGATGGTGAA 
CTTGAGAGTG ATGCTAGTTC TTACAGAGTC CTCTCTAGCA AGACATTGGC AGATGTTGTT 
GAAGCATTAA TTGGTGTTTA TTATGTTGAA GGTGGGAAAA ATGCTGTTAA CCACCTTATG 
AAATGGATTG GAATTCAGGT AGAGTTTGAT CATGAAGAGA TAGATGGTGC ATCTAGGCCA 
TTCAATGTTC CAGAAAGTGT TCTTAGGAGT GTTGACTTTG ATACTTTAGA AGGTGCCTTG 
GATATTAAGT TCAATGATCG TGGCTTGTTG ATAGAAGCCA TAACGCATGC TTCACGACCA 
TCTTCTGGAG TATCCTGCTA TCAACGATTA GAATTTGTTG GTGACGCAGT CTTAGATCAT 
CTTATTACTA GGCACTTGTT TTTTACATAT ACAAATCTGC CCCCTGGTCG CTTGACAGAC 
TTGCGAGCCG CTGCTGTAAA CAATGAAAAT TTTGCACGTG TTGCAGTTAA ACATAAGCTT 
CATGTGCACC TTAGACATGG GTCAAGTGCC CTTGAAAAGC AGATTCGGGA CTTTGTTAGG 
GAAGTTCAAG ATGAACTATT GAAGCCAGTG TTCAACTCCT TTGGTTTGGG AGATTGCAAA 
GCTCCAAAGG TTCTTGGTGA CATCGTTGAA TCCATTGCTG GTGCTATTTT TCTTGACAGC 
GGACGAGATA CAGCGGTTGT GTGGAAGGTT TTTCAACCTC TGTTGCATCC AATGGTCACT 
CCAGAAACCC TGCCAATGCA TCCTGTAAGG GAGCTGCAGG AACGGTGCCA GCAACAGGCT 
GAAGGATTGG AATACAAAGC CACTCGAAGT GGCAATTTGG CTACTGTTGA AGTTTTCATT 
GATGGTGTTC AGGTAGGAGT TGCCCAAAAT CCACAAAAGA AAATGGCACA GAAACTCGCT 
GCTAGGAATG CACTTGTTGT TTTGAAAGAG AAGGAAACAG CTGAAGCCAA GGAAAAGAGT 
GATGAAAATG GGAAGAAGAA AAGGAACGGC AACCAAACCT TTACTAGACA AACATTAAAT 
GATATCTGCT TGCGTAGAAA TTGGCCTATG CCATCATATC GGTGTGTCAA TGAGGGTGGT 
CCTGCACATG CAAAGAGGTT TACTTTTGCA GTTCGTGTGA ATACTACCGA TAGAGGATGG 
ACAGACGAAT GTGTGGGAGA GCCGATGCCG AGTGTTAAGA AGGCCAAAGA CTCTGCTGCA 
GTTCTTCTCT TGGAACTATT AAATAAACGG TACTCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024451582.1
CDS	769..6747
Translation	MESESNGRVSGIGGGGGPSYWLDACEDISCDIIDDFVDFDTSIVPELSVDNNSNVNNDFFGGIDHILDSIKNGSGLPPLHNASTTANVSNGNRDCIVGDGWFINVENGVCHGSSVSQSNGGDKDNIDRKGQVENGGNGLNLSNGKREERFSNNFVKENGKKDEQSTEQGIDGDERCGKRARLCCYRNERVYSSRGEHRDRERCSSRKRSRDWDESDRRDRDISRRRDRYSGSNRRDGRDRDWRERELRGYWERDRSGSKDMVFRLGTWEADHNKEGREANDKIQECKGELEKKSEESKEKVPEEQARQYQLDVLDQAKKKNTIAFLETGAGKTLIAVLLIRSICNDLQRQNKKILAVFLVPKVPLVYQQAEVIRERGYQVGHYCGEMGQDFWDTRRWQREFETKQVLVMTAQILLNILRHSIIKMEAINLLILDECHHAVKKHPYSLVMSEFYHTTPKEKRPSVFGMTASPVNLKGVSSQVDCAIKIRNLESKLDSIVCTIKDRKELEKHVPMPAEVVVEYDKAASLWSLHEQIKQIEAAVEEAAQSSSRRSKWQFMGARDAGAKEELRQVYGVSERTESDGAANLIQKLRAINYALGDLGQWCAYKVAQSFLTALQNDERANYQLDVKFQESYLERVVLLLQCQLTEGAVTDKDTKVSDNGNDNIQDGPGFDEIEEGELPDSHVVSGGEHVDVIIGAAVADGKVTPKVQSLIKVLLRYQHTEDFRAIIFVERVVAALVLPKVFAELPSLSFVRCASLIGHNNSQEMRTSQMQDTIAKFRDGRVTLLVATSVAEEGLDIRQCNVVIRFDLAKTVLAYIQSRGRARKPGSDYILMVERGNLSHGAFLRNARNSEETLRKEAIERTDLSHLKDTSRLIAVDSIPGTVYQVESTGAVVSLNSAVGLVHFYCSQLPSDRYSILRPGFIMEKHEKPGGPTEYSCKLQLPCNAPFEELEGPVCSSMRLAHQAVCLAACKKLHEMGAFTDMLLPDKGSEEEKDKVDQNDEGEPLPGTARHREFYPEGVAKTLQGEWILCGRDGCNNSKVLHLYLYGVRCLNIGTSNDPFLTQVSNFAVLFGNELDAEVYQFIFSFSIDRYFKHFSLTPQFLQVLSMSMDLFIARTMITKASLVFRGRIPITESQLASLKNFHVRLMSIVLDVDVEPSTTPWDPAKAYLFVPMVSDKSVDPIKEIDWDLVENIIGTDAWSNRLQRARPDVYLGTNERTLGGDRREYGFGKLRHGIAFGQKPHPTYGIRGAVAQFDVVKASGLIPKRGWDATETQKLELTKGKLMMADTCVNADALMGRIVTAAHSGKRFYVDSICYDMTAEISFPRKEGYLGPLEYSSYADYYKQKYGVELKFKQQPLLRGRGVSYCKNLLSPRFEHSDSNEGDAEENLDKTYYVFLPPELCLVHPLPGSLVRGAQRLPSIMRRVESMLLAVELKDIINYPVPASKILEALTAASCQETFCYERAELLGDAYLKWVVSRFLFLKYPQKHEGQLTRMRQQMVSNMVLYQYALNKGLQSYIQADRFAPSRWAAPGVLPVFDEETKDGDSYIFDQEKSLAEDRTGMNHLDDGYENEIEDGELESDASSYRVLSSKTLADVVEALIGVYYVEGGKNAVNHLMKWIGIQVEFDHEEIDGASRPFNVPESVLRSVDFDTLEGALDIKFNDRGLLIEAITHASRPSSGVSCYQRLEFVGDAVLDHLITRHLFFTYTNLPPGRLTDLRAAAVNNENFARVAVKHKLHVHLRHGSSALEKQIRDFVREVQDELLKPVFNSFGLGDCKAPKVLGDIVESIAGAIFLDSGRDTAVVWKVFQPLLHPMVTPETLPMHPVRELQERCQQQAEGLEYKATRSGNLATVEVFIDGVQVGVAQNPQKKMAQKLAARNALVVLKEKETAEAKEKSDENGKKKRNGNQTFTRQTLNDICLRRNWPMPSYRCVNEGGPAHAKRFTFAVRVNTTDRGWTDECVGEPMPSVKKAKDSAAVLLLELLNKRYS

Target ORF information:

RefSeq Version	XM_024595814.1
Organism	Populus trichocarpa(black cottonwood)
Definition	Populus trichocarpa endoribonuclease Dicer homolog 1 (LOC18096402), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024595814.1

ORF Insert Sequence:

ATGGAGAGCG AGAGTAATGG TAGGGTTTCG GGGATTGGAG GTGGAGGTGG TCCGTCTTAC 
TGGCTTGATG CATGTGAGGA TATTTCTTGT GATATTATAG ATGATTTTGT TGATTTTGAT 
ACTTCAATTG TTCCAGAATT ATCTGTTGAT AATAATTCCA ATGTTAATAA TGATTTCTTT 
GGAGGGATTG ATCACATTCT TGATAGTATC AAGAATGGTA GTGGTCTTCC TCCTCTTCAT 
AATGCTAGTA CGACAGCTAA TGTTTCCAAT GGAAATCGGG ATTGCATTGT TGGTGATGGT 
TGGTTTATTA ATGTTGAAAA TGGAGTTTGC CATGGGAGCT CGGTTTCACA ATCAAATGGT 
GGTGATAAGG ACAATATTGA CAGAAAAGGG CAGGTGGAGA ATGGTGGTAA TGGGTTGAAT 
TTGTCCAATG GGAAGAGAGA AGAGAGGTTC TCAAACAATT TTGTTAAAGA AAATGGGAAG 
AAGGATGAGC AGTCGACGGA GCAAGGTATT GATGGTGATG AAAGGTGCGG TAAAAGGGCT 
AGACTTTGTT GTTATAGAAA TGAGAGAGTG TATTCTAGTA GAGGGGAGCA TAGGGATAGG 
GAGAGATGTT CTAGCAGGAA GAGGTCACGG GATTGGGATG AAAGCGATAG AAGGGATAGG 
GATATTAGTC GAAGAAGAGA TAGATATAGT GGTAGTAATA GGAGGGATGG TAGAGATAGG 
GATTGGAGGG AAAGAGAACT TAGGGGTTAT TGGGAGAGGG ATCGTTCAGG GTCCAAAGAC 
ATGGTTTTCC GATTGGGCAC TTGGGAAGCT GATCATAACA AAGAAGGAAG GGAGGCAAAT 
GACAAAATTC AGGAGTGCAA AGGAGAGCTA GAGAAGAAAT CAGAGGAGAG TAAAGAGAAA 
GTCCCTGAAG AACAGGCCCG CCAGTATCAA TTGGATGTTC TTGACCAGGC TAAGAAGAAA 
AATACAATAG CTTTTCTTGA AACAGGGGCA GGGAAGACAC TCATTGCTGT ACTCCTCATC 
AGAAGCATTT GCAATGACTT GCAAAGGCAG AATAAAAAAA TTCTAGCTGT ATTTTTAGTT 
CCGAAGGTTC CACTTGTTTA TCAGCAAGCA GAAGTTATTC GTGAGCGCGG TTACCAAGTG 
GGCCATTATT GTGGTGAGAT GGGTCAAGAT TTCTGGGATA CTCGAAGATG GCAGCGTGAG 
TTTGAAACAA AACAGGTTTT AGTAATGACA GCCCAAATTC TTTTGAACAT TCTGAGGCAC 
AGCATAATCA AAATGGAAGC CATAAATCTC CTCATTCTGG ATGAGTGTCA TCATGCTGTG 
AAGAAACATC CATATTCTCT TGTGATGTCT GAGTTTTACC ATACGACTCC CAAGGAGAAG 
AGACCGTCTG TTTTTGGAAT GACAGCTTCT CCTGTTAACT TAAAGGGTGT TTCAAGTCAG 
GTGGATTGTG CAATAAAGAT CCGCAATCTT GAAAGTAAAC TGGACTCAAT TGTTTGTACG 
ATAAAAGACC GCAAGGAACT GGAAAAGCAT GTACCAATGC CGGCAGAGGT TGTGGTGGAG 
TACGACAAAG CAGCTAGCTT GTGGTCCCTT CATGAACAAA TAAAGCAAAT CGAAGCAGCT 
GTTGAGGAAG CTGCACAGTC TAGCTCGAGA AGAAGTAAAT GGCAATTTAT GGGAGCTAGG 
GATGCTGGGG CCAAGGAAGA GCTGCGCCAG GTTTATGGTG TCTCTGAAAG AACAGAAAGT 
GATGGGGCTG CTAACTTAAT TCAAAAGTTG AGGGCAATTA ATTATGCACT TGGTGATCTG 
GGTCAGTGGT GTGCTTACAA GGTTGCACAA TCCTTTCTGA CTGCCTTACA AAATGACGAG 
AGAGCAAACT ATCAGCTTGA TGTTAAGTTT CAAGAATCCT ACCTCGAAAG AGTCGTGTTG 
CTCTTACAAT GCCAATTAAC AGAGGGAGCT GTTACAGACA AGGATACAAA AGTTTCAGAC 
AATGGGAATG ACAATATTCA GGATGGGCCT GGTTTTGATG AGATTGAGGA GGGAGAGCTC 
CCTGACAGTC ATGTTGTCTC TGGTGGAGAA CATGTTGATG TGATAATAGG AGCTGCTGTA 
GCTGATGGAA AAGTGACCCC TAAAGTGCAG TCACTGATTA AAGTACTTCT CAGATATCAG 
CACACGGAGG ATTTTCGTGC GATCATCTTT GTTGAGCGCG TAGTAGCTGC TTTGGTTCTT 
CCTAAGGTCT TTGCAGAGCT TCCATCTCTA AGTTTTGTTA GGTGTGCAAG TTTGATAGGG 
CACAATAATA GTCAGGAAAT GAGAACAAGC CAAATGCAAG ACACAATTGC CAAATTTCGA 
GATGGTCGTG TGACATTGTT AGTTGCTACC AGTGTTGCTG AGGAAGGACT TGATATTAGG 
CAATGCAATG TTGTTATTCG CTTTGATCTT GCAAAAACTG TTCTTGCATA TATTCAGTCT 
CGAGGTCGTG CAAGAAAGCC AGGCTCTGAT TACATCTTGA TGGTGGAGAG GGGAAACCTG 
TCTCATGGAG CATTCTTAAG GAATGCTAGG AATAGTGAGG AGACCCTGAG GAAAGAAGCG 
ATTGAGAGAA CTGACCTCAG TCATCTTAAG GATACTTCAA GGTTAATTGC AGTAGATTCA 
ATACCAGGAA CTGTGTATCA GGTGGAGTCT ACTGGTGCTG TTGTGAGCTT AAATTCTGCT 
GTTGGACTTG TTCATTTCTA TTGCTCTCAG TTACCCAGTG ACAGGTATTC AATACTTCGT 
CCTGGGTTTA TTATGGAGAA GCATGAGAAG CCAGGAGGTC CCACGGAATA TTCATGCAAG 
CTTCAGCTTC CTTGTAATGC ACCATTTGAG GAGCTTGAGG GTCCTGTATG CAGTTCAATG 
CGCCTTGCAC ATCAGGCTGT TTGTTTGGCT GCTTGCAAGA AGCTTCATGA GATGGGAGCA 
TTTACTGACA TGCTCTTGCC AGACAAGGGA AGTGAGGAAG AAAAGGACAA GGTTGACCAG 
AATGATGAAG GTGAACCACT TCCTGGTACT GCTAGGCATC GGGAGTTCTA TCCTGAAGGT 
GTAGCTAAGA CACTCCAGGG AGAATGGATC TTATGCGGAA GGGATGGTTG CAACAACTCC 
AAAGTGCTTC ATCTCTACTT GTATGGAGTC AGATGCTTAA ACATTGGCAC TTCAAATGAT 
CCATTCTTGA CTCAAGTTTC AAATTTTGCA GTGCTTTTTG GCAATGAGCT GGATGCAGAG 
GTATATCAAT TTATTTTCAG TTTCAGCATA GATAGATATT TTAAGCATTT TTCTTTAACA 
CCTCAATTTC TTCAGGTCTT ATCGATGTCG ATGGATCTAT TTATCGCTCG AACCATGATA 
ACGAAGGCAT CTCTTGTCTT TAGGGGTCGC ATACCTATTA CAGAAAGTCA GCTGGCATCC 
CTTAAAAATT TTCATGTAAG GTTGATGAGC ATTGTACTGG ATGTGGATGT TGAACCTTCC 
ACCACTCCAT GGGATCCTGC AAAAGCTTAT TTATTCGTCC CTATGGTTAG CGATAAGTCT 
GTAGATCCTA TAAAAGAAAT TGACTGGGAT CTGGTTGAAA ATATAATTGG AACAGATGCT 
TGGAGCAATC GTCTTCAGAG AGCTCGACCA GATGTTTACC TTGGTACAAA TGAGAGGACA 
CTTGGTGGAG ACAGGAGGGA GTATGGTTTT GGGAAATTGC GACATGGAAT TGCTTTTGGA 
CAGAAACCTC ATCCCACTTA TGGCATCAGG GGAGCTGTAG CACAGTTTGA TGTTGTGAAA 
GCTTCTGGAT TGATTCCTAA GAGGGGATGG GATGCAACTG AGACGCAAAA ACTGGAGCTG 
ACCAAAGGCA AATTGATGAT GGCTGATACT TGTGTCAATG CTGATGCTTT GATGGGGAGA 
ATTGTAACAG CTGCTCACTC TGGGAAGAGG TTTTATGTGG ATTCCATATG CTATGATATG 
ACTGCAGAGA TCTCATTTCC AAGAAAAGAG GGTTATCTTG GTCCTCTGGA GTACAGCTCA 
TATGCTGATT ACTACAAGCA AAAGTATGGA GTTGAATTGA AATTTAAGCA GCAGCCTTTA 
CTAAGAGGAC GTGGTGTTTC ATACTGCAAG AATCTCTTAT CTCCTCGGTT TGAACACTCA 
GATTCAAATG AAGGCGATGC TGAAGAGAAT CTTGACAAGA CTTACTATGT GTTTCTTCCT 
CCTGAGCTGT GTCTGGTGCA CCCTCTTCCT GGATCACTTG TTCGAGGTGC CCAGAGATTA 
CCATCGATAA TGAGGAGGGT TGAAAGCATG CTGCTCGCTG TTGAACTTAA GGATATAATA 
AATTATCCAG TCCCTGCTTC AAAGATCTTG GAAGCCTTGA CTGCTGCTTC ATGCCAAGAG 
ACATTCTGCT ATGAAAGAGC AGAGCTTCTA GGAGATGCTT ACCTGAAATG GGTGGTTAGT 
CGATTTCTTT TTCTGAAGTA TCCCCAGAAA CATGAGGGTC AACTTACTAG AATGAGGCAA 
CAAATGGTAA GTAATATGGT TTTGTATCAA TATGCACTTA ACAAAGGACT TCAGTCATAT 
ATCCAAGCAG ATCGCTTTGC TCCATCTCGA TGGGCTGCTC CTGGAGTGTT GCCTGTCTTT 
GATGAGGAAA CAAAAGATGG AGACTCGTAC ATATTTGATC AGGAGAAGTC ACTTGCTGAG 
GATAGAACCG GAATGAATCA TCTTGATGAT GGGTATGAAA ATGAAATTGA AGATGGTGAA 
CTTGAGAGTG ATGCTAGTTC TTACAGAGTC CTCTCTAGCA AGACATTGGC AGATGTTGTT 
GAAGCATTAA TTGGTGTTTA TTATGTTGAA GGTGGGAAAA ATGCTGTTAA CCACCTTATG 
AAATGGATTG GAATTCAGGT AGAGTTTGAT CATGAAGAGA TAGATGGTGC ATCTAGGCCA 
TTCAATGTTC CAGAAAGTGT TCTTAGGAGT GTTGACTTTG ATACTTTAGA AGGTGCCTTG 
GATATTAAGT TCAATGATCG TGGCTTGTTG ATAGAAGCCA TAACGCATGC TTCACGACCA 
TCTTCTGGAG TATCCTGCTA TCAACGATTA GAATTTGTTG GTGACGCAGT CTTAGATCAT 
CTTATTACTA GGCACTTGTT TTTTACATAT ACAAATCTGC CCCCTGGTCG CTTGACAGAC 
TTGCGAGCCG CTGCTGTAAA CAATGAAAAT TTTGCACGTG TTGCAGTTAA ACATAAGCTT 
CATGTGCACC TTAGACATGG GTCAAGTGCC CTTGAAAAGC AGATTCGGGA CTTTGTTAGG 
GAAGTTCAAG ATGAACTATT GAAGCCAGTG TTCAACTCCT TTGGTTTGGG AGATTGCAAA 
GCTCCAAAGG TTCTTGGTGA CATCGTTGAA TCCATTGCTG GTGCTATTTT TCTTGACAGC 
GGACGAGATA CAGCGGTTGT GTGGAAGGTT TTTCAACCTC TGTTGCATCC AATGGTCACT 
CCAGAAACCC TGCCAATGCA TCCTGTAAGG GAGCTGCAGG AACGGTGCCA GCAACAGGCT 
GAAGGATTGG AATACAAAGC CACTCGAAGT GGCAATTTGG CTACTGTTGA AGTTTTCATT 
GATGGTGTTC AGGTAGGAGT TGCCCAAAAT CCACAAAAGA AAATGGCACA GAAACTCGCT 
GCTAGGAATG CACTTGTTGT TTTGAAAGAG AAGGAAACAG CTGAAGCCAA GGAAAAGAGT 
GATGAAAATG GGAAGAAGAA AAGGAACGGC AACCAAACCT TTACTAGACA AACATTAAAT 
GATATCTGCT TGCGTAGAAA TTGGCCTATG CCATCATATC GGTGTGTCAA TGAGGGTGGT 
CCTGCACATG CAAAGAGGTT TACTTTTGCA GTTCGTGTGA ATACTACCGA TAGAGGATGG 
ACAGACGAAT GTGTGGGAGA GCCGATGCCG AGTGTTAAGA AGGCCAAAGA CTCTGCTGCA 
GTTCTTCTCT TGGAACTATT AAATAAACGG TACTCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC18096402 ( XM_006386606.1 ) cDNA ORF clone, Populus trichocarpa(black cottonwood) -> XP_006386668.1 Populus trichocarpa Endoribonuclease Dicer family protein (POPTR_0002s18240g) mRNA, complete cds.

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CloneID	OPa07043
Clone ID Related Accession (Same CDS sequence)	XM_006386606.1 , XM_006386606.2
Accession Version	XM_006386606.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5904bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2013-12-30
Organism	Populus trichocarpa(Populus balsamifera subsp. trichocarpa)
Product	Endoribonuclease Dicer family protein
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_008468). COMPLETENESS: incomplete on the 5' end.

ATGGAGAGCG AGAGTAATGG TAGGGTTTCG GGGATTGGAG GTGGAGGTGG TCCGTCTTAC 
TGGCTTGATG CATGTGAGGA TATTTCTTGT GATATTATAG ATGATTTTGT TGATTTTGAT 
ACTTCAATTG TTCCAGAATT ATCTGTTGAT AATAATTCCA ATGTTAATAA TGATTTCTTT 
GGAGGGATTG ATCACATTCT TGATAGTATC AAGAATGGTA GTGGTCTTCC TCCTCTTCAT 
AATGCTAGTA CGACAGCTAA TGTTTCCAAT GGAAATCGGG ATTGCATTGT TGGTGATGGT 
TGGTTTATTA ATGTTGAAAA TGGAGTTTGC CATGGGAGCT CGGTTTCACA ATCAAATGGT 
GGTGATAAGG ACAATATTGA CAGAAAAGGG CAGGTGGAGA ATGGTGGTAA TGGGTTGAAT 
TTGTCCAATG GGAAGAGAGA AGAGAGGTTC TCAAACAATT TTGTTAAAGA AAATGGGAAG 
AAGGATGAGC AGTCGACGGA GCAAGGTATT GATGGTGATG AAAGGTGCGG TAAAAGGGCT 
AGACTTTGTT GTTATAGAAA TGAGAGAGTG TATTCTAGTA GAGGGGAGCA TAGGGATAGG 
GAGAGATGTT CTAGCAGGAA GAGGTCACGG GATTGGGATG AAAGCGATAG AAGGGATAGG 
GATATTAGTC GAAGAAGAGA TAGATATAGT GGTAGTAATA GGAGGGATGG TAGAGATAGG 
GATTGGAGGG AAAGAGAACT TAGGGGTTAT TGGGAGAGGG ATCGTTCAGG GTCCAAAGAC 
ATGGTTTTCC GATTGGGCAC TTGGGAAGCT GATCATAACA AAGAAGGAAG GGAGGCAAAT 
GACAAAATTC AGGAGTGCAA AGGAGAGCTA GAGAAGAAAT CAGAGGAGAG TAAAGAGAAA 
GTCCCTGAAG AACAGGCCCG CCAGTATCAA TTGGATGTTC TTGACCAGGC TAAGAAGAAA 
AATACAATAG CTTTTCTTGA AACAGGGGCA GGGAAGACAC TCATTGCTGT ACTCCTCATC 
AGAAGCATTT GCAATGACTT GCAAAGGCAG AATAAAAAAA TTCTAGCTGT ATTTTTAGTT 
CCGAAGGTTC CACTTGTTTA TCAGCAAGCA GAAGTTATTC GTGAGCGCGG TTACCAAGTG 
GGCCATTATT GTGGTGAGAT GGGTCAAGAT TTCTGGGATA CTCGAAGATG GCAGCGTGAG 
TTTGAAACAA AACAGGTTTT AGTAATGACA GCCCAAATTC TTTTGAACAT TCTGAGGCAC 
AGCATAATCA AAATGGAAGC CATAAATCTC CTCATTCTGG ATGAGTGTCA TCATGCTGTG 
AAGAAACATC CATATTCTCT TGTGATGTCT GAGTTTTACC ATACGACTCC CAAGGAGAAG 
AGACCGTCTG TTTTTGGAAT GACAGCTTCT CCTGTTAACT TAAAGGGTGT TTCAAGTCAG 
GTGGATTGTG CAATAAAGAT CCGCAATCTT GAAAGTAAAC TGGACTCAAT TGTTTGTACG 
ATAAAAGACC GCAAGGAACT GGAAAAGCAT GTACCAATGC CGGCAGAGGT TGTGGTGGAG 
TACGACAAAG CAGCTAGCTT GTGGTCCCTT CATGAACAAA TAAAGCAAAT CGAAGCAGCT 
GTTGAGGAAG CTGCACAGTC TAGCTCGAGA AGAAGTAAAT GGCAATTTAT GGGAGCTAGG 
GATGCTGGGG CCAAGGAAGA GCTGCGCCAG GTTTATGGTG TCTCTGAAAG AACAGAAAGT 
GATGGGGCTG CTAACTTAAT TCAAAAGTTG AGGGCAATTA ATTATGCACT TGGTGATCTG 
GGTCAGTGGT GTGCTTACAA GGTTGCACAA TCCTTTCTGA CTGCCTTACA AAATGACGAG 
AGAGCAAACT ATCAGCTTGA TGTTAAGTTT CAAGAATCCT ACCTCGAAAG AGTCGTGTTG 
CTCTTACAAT GCCAATTAAC AGAGGGAGCT GTTACAGACA AGGATACAAA AGTTTCAGAC 
AATGGGAATG ACAATATTCA GGATGGGCCT GGTTTTGATG AGATTGAGGA GGGAGAGCTC 
CCTGACAGTC ATGTTGTCTC TGGTGGAGAA CATGTTGATG TGATAATAGG AGCTGCTGTA 
GCTGATGGAA AAGTGACCCC TAAAGTGCAG TCACTGATTA AAGTACTTCT CAGATATCAG 
CACACGGAGG ATTTTCGTGC GATCATCTTT GTTGAGCGCG TAGTAGCTGC TTTGGTTCTT 
CCTAAGGTCT TTGCAGAGCT TCCATCTCTA AGTTTTGTTA GGTGTGCAAG TTTGATAGGG 
CACAATAATA GTCAGGAAAT GAGAACAAGC CAAATGCAAG ACACAATTGC CAAATTTCGA 
GATGGTCGTG TGACATTGTT AGTTGCTACC AGTGTTGCTG AGGAAGGACT TGATATTAGG 
CAATGCAATG TTGTTATTCG CTTTGATCTT GCAAAAACTG TTCTTGCATA TATTCAGTCT 
CGAGGTCGTG CAAGAAAGCC AGGCTCTGAT TACATCTTGA TGGTGGAGAG GGGAAACCTG 
TCTCATGGAG CATTCTTAAG GAATGCTAGG AATAGTGAGG AGACCCTGAG GAAAGAAGCG 
ATTGAGAGAA CTGACCTCAG TCATCTTAAG GATACTTCAA GGTTAATTGC AGTAGATTCA 
ATACCAGGAA CTGTGTATCA GGTGGAGTCT ACTGGTGCTG TTGTGAGCTT AAATTCTGCT 
GTTGGACTTG TTCATTTCTA TTGCTCTCAG TTACCCAGTG ACAGGTATTC AATACTTCGT 
CCTGGGTTTA TTATGGAGAA GCATGAGAAG CCAGGAGGTC CCACGGAATA TTCATGCAAG 
CTTCAGCTTC CTTGTAATGC ACCATTTGAG GAGCTTGAGG GTCCTGTATG CAGTTCAATG 
CGCCTTGCAC ATCAGGCTGT TTGTTTGGCT GCTTGCAAGA AGCTTCATGA GATGGGAGCA 
TTTACTGACA TGCTCTTGCC AGACAAGGGA AGTGAGGAAG AAAAGGACAA GGTTGACCAG 
AATGATGAAG GTGAACCACT TCCTGGTACT GCTAGGCATC GGGAGTTCTA TCCTGAAGGT 
GTAGCTAAGA CACTCCAGGG AGAATGGATC TTATGCGGAA GGGATGGTTG CAACAACTCC 
AAAGTGCTTC ATCTCTACTT GTATGGAGTC AGATGCTTAA ACATTGGCAC TTCAAATGAT 
CCATTCTTGA CTCAAGTTTC AAATTTTGCA GTGCTTTTTG GCAATGAGCT GGATGCAGAG 
GTCTTATCGA TGTCGATGGA TCTATTTATC GCTCGAACCA TGATAACGAA GGCATCTCTT 
GTCTTTAGGG GTCGCATACC TATTACAGAA AGTCAGCTGG CATCCCTTAA AAATTTTCAT 
GTAAGGTTGA TGAGCATTGT ACTGGATGTG GATGTTGAAC CTTCCACCAC TCCATGGGAT 
CCTGCAAAAG CTTATTTATT CGTCCCTATG GTTAGCGATA AGTCTGTAGA TCCTATAAAA 
GAAATTGACT GGGATCTGGT TGAAAATATA ATTGGAACAG ATGCTTGGAG CAATCGTCTT 
CAGAGAGCTC GACCAGATGT TTACCTTGGT ACAAATGAGA GGACACTTGG TGGAGACAGG 
AGGGAGTATG GTTTTGGGAA ATTGCGACAT GGAATTGCTT TTGGACAGAA ACCTCATCCC 
ACTTATGGCA TCAGGGGAGC TGTAGCACAG TTTGATGTTG TGAAAGCTTC TGGATTGATT 
CCTAAGAGGG GATGGGATGC AACTGAGACG CAAAAACTGG AGCTGACCAA AGGCAAATTG 
ATGATGGCTG ATACTTGTGT CAATGCTGAT GCTTTGATGG GGAGAATTGT AACAGCTGCT 
CACTCTGGGA AGAGGTTTTA TGTGGATTCC ATATGCTATG ATATGACTGC AGAGATCTCA 
TTTCCAAGAA AAGAGGGTTA TCTTGGTCCT CTGGAGTACA GCTCATATGC TGATTACTAC 
AAGCAAAAGT ATGGAGTTGA ATTGAAATTT AAGCAGCAGC CTTTACTAAG AGGACGTGGT 
GTTTCATACT GCAAGAATCT CTTATCTCCT CGGTTTGAAC ACTCAGATTC AAATGAAGGC 
GATGCTGAAG AGAATCTTGA CAAGACTTAC TATGTGTTTC TTCCTCCTGA GCTGTGTCTG 
GTGCACCCTC TTCCTGGATC ACTTGTTCGA GGTGCCCAGA GATTACCATC GATAATGAGG 
AGGGTTGAAA GCATGCTGCT CGCTGTTGAA CTTAAGGATA TAATAAATTA TCCAGTCCCT 
GCTTCAAAGA TCTTGGAAGC CTTGACTGCT GCTTCATGCC AAGAGACATT CTGCTATGAA 
AGAGCAGAGC TTCTAGGAGA TGCTTACCTG AAATGGGTGG TTAGTCGATT TCTTTTTCTG 
AAGTATCCCC AGAAACATGA GGGTCAACTT ACTAGAATGA GGCAACAAAT GGTAAGTAAT 
ATGGTTTTGT ATCAATATGC ACTTAACAAA GGACTTCAGT CATATATCCA AGCAGATCGC 
TTTGCTCCAT CTCGATGGGC TGCTCCTGGA GTGTTGCCTG TCTTTGATGA GGAAACAAAA 
GATGGAGACT CGTACATATT TGATCAGGAG AAGTCACTTG CTGAGGATAG AACCGGAATG 
AATCATCTTG ATGATGGGTA TGAAAATGAA ATTGAAGATG GTGAACTTGA GAGTGATGCT 
AGTTCTTACA GAGTCCTCTC TAGCAAGACA TTGGCAGATG TTGTTGAAGC ATTAATTGGT 
GTTTATTATG TTGAAGGTGG GAAAAATGCT GTTAACCACC TTATGAAATG GATTGGAATT 
CAGGTAGAGT TTGATCATGA AGAGATAGAT GGTGCATCTA GGCCATTCAA TGTTCCAGAA 
AGTGTTCTTA GGAGTGTTGA CTTTGATACT TTAGAAGGTG CCTTGGATAT TAAGTTCAAT 
GATCGTGGCT TGTTGATAGA AGCCATAACG CATGCTTCAC GACCATCTTC TGGAGTATCC 
TGCTATCAAC GATTAGAATT TGTTGGTGAC GCAGTCTTAG ATCATCTTAT TACTAGGCAC 
TTGTTTTTTA CATATACAAA TCTGCCCCCT GGTCGCTTGA CAGACTTGCG AGCCGCTGCT 
GTAAACAATG AAAATTTTGC ACGTGTTGCA GTTAAACATA AGCTTCATGT GCACCTTAGA 
CATGGGTCAA GTGCCCTTGA AAAGCAGATT CGGGACTTTG TTAGGGAAGT TCAAGATGAA 
CTATTGAAGC CAGTGTTCAA CTCCTTTGGT TTGGGAGATT GCAAAGCTCC AAAGGTTCTT 
GGTGACATCG TTGAATCCAT TGCTGGTGCT ATTTTTCTTG ACAGCGGACG AGATACAGCG 
GTTGTGTGGA AGGTTTTTCA ACCTCTGTTG CATCCAATGG TCACTCCAGA AACCCTGCCA 
ATGCATCCTG TAAGGGAGCT GCAGGAACGG TGCCAGCAAC AGGCTGAAGG ATTGGAATAC 
AAAGCCACTC GAAGTGGCAA TTTGGCTACT GTTGAAGTTT TCATTGATGG TGTTCAGGTA 
GGAGTTGCCC AAAATCCACA AAAGAAAATG GCACAGAAAC TCGCTGCTAG GAATGCACTT 
GTTGTTTTGA AAGAGAAGGA AACAGCTGAA GCCAAGGAAA AGAGTGATGA AAATGGGAAG 
AAGAAAAGGA ACGGCAACCA AACCTTTACT AGACAAACAT TAAATGATAT CTGCTTGCGT 
AGAAATTGGC CTATGCCATC ATATCGGTGT GTCAATGAGG GTGGTCCTGC ACATGCAAAG 
AGGTTTACTT TTGCAGTTCG TGTGAATACT ACCGATAGAG GATGGACAGA CGAATGTGTG 
GGAGAGCCGA TGCCGAGTGT TAAGAAGGCC AAAGACTCTG CTGCAGTTCT TCTCTTGGAA 
CTATTAAATA AACGGTACTC ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006386668.1
CDS	1..5904
Translation	MESESNGRVSGIGGGGGPSYWLDACEDISCDIIDDFVDFDTSIVPELSVDNNSNVNNDFFGGIDHILDSIKNGSGLPPLHNASTTANVSNGNRDCIVGDGWFINVENGVCHGSSVSQSNGGDKDNIDRKGQVENGGNGLNLSNGKREERFSNNFVKENGKKDEQSTEQGIDGDERCGKRARLCCYRNERVYSSRGEHRDRERCSSRKRSRDWDESDRRDRDISRRRDRYSGSNRRDGRDRDWRERELRGYWERDRSGSKDMVFRLGTWEADHNKEGREANDKIQECKGELEKKSEESKEKVPEEQARQYQLDVLDQAKKKNTIAFLETGAGKTLIAVLLIRSICNDLQRQNKKILAVFLVPKVPLVYQQAEVIRERGYQVGHYCGEMGQDFWDTRRWQREFETKQVLVMTAQILLNILRHSIIKMEAINLLILDECHHAVKKHPYSLVMSEFYHTTPKEKRPSVFGMTASPVNLKGVSSQVDCAIKIRNLESKLDSIVCTIKDRKELEKHVPMPAEVVVEYDKAASLWSLHEQIKQIEAAVEEAAQSSSRRSKWQFMGARDAGAKEELRQVYGVSERTESDGAANLIQKLRAINYALGDLGQWCAYKVAQSFLTALQNDERANYQLDVKFQESYLERVVLLLQCQLTEGAVTDKDTKVSDNGNDNIQDGPGFDEIEEGELPDSHVVSGGEHVDVIIGAAVADGKVTPKVQSLIKVLLRYQHTEDFRAIIFVERVVAALVLPKVFAELPSLSFVRCASLIGHNNSQEMRTSQMQDTIAKFRDGRVTLLVATSVAEEGLDIRQCNVVIRFDLAKTVLAYIQSRGRARKPGSDYILMVERGNLSHGAFLRNARNSEETLRKEAIERTDLSHLKDTSRLIAVDSIPGTVYQVESTGAVVSLNSAVGLVHFYCSQLPSDRYSILRPGFIMEKHEKPGGPTEYSCKLQLPCNAPFEELEGPVCSSMRLAHQAVCLAACKKLHEMGAFTDMLLPDKGSEEEKDKVDQNDEGEPLPGTARHREFYPEGVAKTLQGEWILCGRDGCNNSKVLHLYLYGVRCLNIGTSNDPFLTQVSNFAVLFGNELDAEVLSMSMDLFIARTMITKASLVFRGRIPITESQLASLKNFHVRLMSIVLDVDVEPSTTPWDPAKAYLFVPMVSDKSVDPIKEIDWDLVENIIGTDAWSNRLQRARPDVYLGTNERTLGGDRREYGFGKLRHGIAFGQKPHPTYGIRGAVAQFDVVKASGLIPKRGWDATETQKLELTKGKLMMADTCVNADALMGRIVTAAHSGKRFYVDSICYDMTAEISFPRKEGYLGPLEYSSYADYYKQKYGVELKFKQQPLLRGRGVSYCKNLLSPRFEHSDSNEGDAEENLDKTYYVFLPPELCLVHPLPGSLVRGAQRLPSIMRRVESMLLAVELKDIINYPVPASKILEALTAASCQETFCYERAELLGDAYLKWVVSRFLFLKYPQKHEGQLTRMRQQMVSNMVLYQYALNKGLQSYIQADRFAPSRWAAPGVLPVFDEETKDGDSYIFDQEKSLAEDRTGMNHLDDGYENEIEDGELESDASSYRVLSSKTLADVVEALIGVYYVEGGKNAVNHLMKWIGIQVEFDHEEIDGASRPFNVPESVLRSVDFDTLEGALDIKFNDRGLLIEAITHASRPSSGVSCYQRLEFVGDAVLDHLITRHLFFTYTNLPPGRLTDLRAAAVNNENFARVAVKHKLHVHLRHGSSALEKQIRDFVREVQDELLKPVFNSFGLGDCKAPKVLGDIVESIAGAIFLDSGRDTAVVWKVFQPLLHPMVTPETLPMHPVRELQERCQQQAEGLEYKATRSGNLATVEVFIDGVQVGVAQNPQKKMAQKLAARNALVVLKEKETAEAKEKSDENGKKKRNGNQTFTRQTLNDICLRRNWPMPSYRCVNEGGPAHAKRFTFAVRVNTTDRGWTDECVGEPMPSVKKAKDSAAVLLLELLNKRYS

Target ORF information:

RefSeq Version	XM_006386606.1
Organism	Populus trichocarpa(Populus balsamifera subsp. trichocarpa)
Definition	Populus trichocarpa Endoribonuclease Dicer family protein (POPTR_0002s18240g) mRNA, complete cds.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006386606.1

ORF Insert Sequence:

ATGGAGAGCG AGAGTAATGG TAGGGTTTCG GGGATTGGAG GTGGAGGTGG TCCGTCTTAC 
TGGCTTGATG CATGTGAGGA TATTTCTTGT GATATTATAG ATGATTTTGT TGATTTTGAT 
ACTTCAATTG TTCCAGAATT ATCTGTTGAT AATAATTCCA ATGTTAATAA TGATTTCTTT 
GGAGGGATTG ATCACATTCT TGATAGTATC AAGAATGGTA GTGGTCTTCC TCCTCTTCAT 
AATGCTAGTA CGACAGCTAA TGTTTCCAAT GGAAATCGGG ATTGCATTGT TGGTGATGGT 
TGGTTTATTA ATGTTGAAAA TGGAGTTTGC CATGGGAGCT CGGTTTCACA ATCAAATGGT 
GGTGATAAGG ACAATATTGA CAGAAAAGGG CAGGTGGAGA ATGGTGGTAA TGGGTTGAAT 
TTGTCCAATG GGAAGAGAGA AGAGAGGTTC TCAAACAATT TTGTTAAAGA AAATGGGAAG 
AAGGATGAGC AGTCGACGGA GCAAGGTATT GATGGTGATG AAAGGTGCGG TAAAAGGGCT 
AGACTTTGTT GTTATAGAAA TGAGAGAGTG TATTCTAGTA GAGGGGAGCA TAGGGATAGG 
GAGAGATGTT CTAGCAGGAA GAGGTCACGG GATTGGGATG AAAGCGATAG AAGGGATAGG 
GATATTAGTC GAAGAAGAGA TAGATATAGT GGTAGTAATA GGAGGGATGG TAGAGATAGG 
GATTGGAGGG AAAGAGAACT TAGGGGTTAT TGGGAGAGGG ATCGTTCAGG GTCCAAAGAC 
ATGGTTTTCC GATTGGGCAC TTGGGAAGCT GATCATAACA AAGAAGGAAG GGAGGCAAAT 
GACAAAATTC AGGAGTGCAA AGGAGAGCTA GAGAAGAAAT CAGAGGAGAG TAAAGAGAAA 
GTCCCTGAAG AACAGGCCCG CCAGTATCAA TTGGATGTTC TTGACCAGGC TAAGAAGAAA 
AATACAATAG CTTTTCTTGA AACAGGGGCA GGGAAGACAC TCATTGCTGT ACTCCTCATC 
AGAAGCATTT GCAATGACTT GCAAAGGCAG AATAAAAAAA TTCTAGCTGT ATTTTTAGTT 
CCGAAGGTTC CACTTGTTTA TCAGCAAGCA GAAGTTATTC GTGAGCGCGG TTACCAAGTG 
GGCCATTATT GTGGTGAGAT GGGTCAAGAT TTCTGGGATA CTCGAAGATG GCAGCGTGAG 
TTTGAAACAA AACAGGTTTT AGTAATGACA GCCCAAATTC TTTTGAACAT TCTGAGGCAC 
AGCATAATCA AAATGGAAGC CATAAATCTC CTCATTCTGG ATGAGTGTCA TCATGCTGTG 
AAGAAACATC CATATTCTCT TGTGATGTCT GAGTTTTACC ATACGACTCC CAAGGAGAAG 
AGACCGTCTG TTTTTGGAAT GACAGCTTCT CCTGTTAACT TAAAGGGTGT TTCAAGTCAG 
GTGGATTGTG CAATAAAGAT CCGCAATCTT GAAAGTAAAC TGGACTCAAT TGTTTGTACG 
ATAAAAGACC GCAAGGAACT GGAAAAGCAT GTACCAATGC CGGCAGAGGT TGTGGTGGAG 
TACGACAAAG CAGCTAGCTT GTGGTCCCTT CATGAACAAA TAAAGCAAAT CGAAGCAGCT 
GTTGAGGAAG CTGCACAGTC TAGCTCGAGA AGAAGTAAAT GGCAATTTAT GGGAGCTAGG 
GATGCTGGGG CCAAGGAAGA GCTGCGCCAG GTTTATGGTG TCTCTGAAAG AACAGAAAGT 
GATGGGGCTG CTAACTTAAT TCAAAAGTTG AGGGCAATTA ATTATGCACT TGGTGATCTG 
GGTCAGTGGT GTGCTTACAA GGTTGCACAA TCCTTTCTGA CTGCCTTACA AAATGACGAG 
AGAGCAAACT ATCAGCTTGA TGTTAAGTTT CAAGAATCCT ACCTCGAAAG AGTCGTGTTG 
CTCTTACAAT GCCAATTAAC AGAGGGAGCT GTTACAGACA AGGATACAAA AGTTTCAGAC 
AATGGGAATG ACAATATTCA GGATGGGCCT GGTTTTGATG AGATTGAGGA GGGAGAGCTC 
CCTGACAGTC ATGTTGTCTC TGGTGGAGAA CATGTTGATG TGATAATAGG AGCTGCTGTA 
GCTGATGGAA AAGTGACCCC TAAAGTGCAG TCACTGATTA AAGTACTTCT CAGATATCAG 
CACACGGAGG ATTTTCGTGC GATCATCTTT GTTGAGCGCG TAGTAGCTGC TTTGGTTCTT 
CCTAAGGTCT TTGCAGAGCT TCCATCTCTA AGTTTTGTTA GGTGTGCAAG TTTGATAGGG 
CACAATAATA GTCAGGAAAT GAGAACAAGC CAAATGCAAG ACACAATTGC CAAATTTCGA 
GATGGTCGTG TGACATTGTT AGTTGCTACC AGTGTTGCTG AGGAAGGACT TGATATTAGG 
CAATGCAATG TTGTTATTCG CTTTGATCTT GCAAAAACTG TTCTTGCATA TATTCAGTCT 
CGAGGTCGTG CAAGAAAGCC AGGCTCTGAT TACATCTTGA TGGTGGAGAG GGGAAACCTG 
TCTCATGGAG CATTCTTAAG GAATGCTAGG AATAGTGAGG AGACCCTGAG GAAAGAAGCG 
ATTGAGAGAA CTGACCTCAG TCATCTTAAG GATACTTCAA GGTTAATTGC AGTAGATTCA 
ATACCAGGAA CTGTGTATCA GGTGGAGTCT ACTGGTGCTG TTGTGAGCTT AAATTCTGCT 
GTTGGACTTG TTCATTTCTA TTGCTCTCAG TTACCCAGTG ACAGGTATTC AATACTTCGT 
CCTGGGTTTA TTATGGAGAA GCATGAGAAG CCAGGAGGTC CCACGGAATA TTCATGCAAG 
CTTCAGCTTC CTTGTAATGC ACCATTTGAG GAGCTTGAGG GTCCTGTATG CAGTTCAATG 
CGCCTTGCAC ATCAGGCTGT TTGTTTGGCT GCTTGCAAGA AGCTTCATGA GATGGGAGCA 
TTTACTGACA TGCTCTTGCC AGACAAGGGA AGTGAGGAAG AAAAGGACAA GGTTGACCAG 
AATGATGAAG GTGAACCACT TCCTGGTACT GCTAGGCATC GGGAGTTCTA TCCTGAAGGT 
GTAGCTAAGA CACTCCAGGG AGAATGGATC TTATGCGGAA GGGATGGTTG CAACAACTCC 
AAAGTGCTTC ATCTCTACTT GTATGGAGTC AGATGCTTAA ACATTGGCAC TTCAAATGAT 
CCATTCTTGA CTCAAGTTTC AAATTTTGCA GTGCTTTTTG GCAATGAGCT GGATGCAGAG 
GTCTTATCGA TGTCGATGGA TCTATTTATC GCTCGAACCA TGATAACGAA GGCATCTCTT 
GTCTTTAGGG GTCGCATACC TATTACAGAA AGTCAGCTGG CATCCCTTAA AAATTTTCAT 
GTAAGGTTGA TGAGCATTGT ACTGGATGTG GATGTTGAAC CTTCCACCAC TCCATGGGAT 
CCTGCAAAAG CTTATTTATT CGTCCCTATG GTTAGCGATA AGTCTGTAGA TCCTATAAAA 
GAAATTGACT GGGATCTGGT TGAAAATATA ATTGGAACAG ATGCTTGGAG CAATCGTCTT 
CAGAGAGCTC GACCAGATGT TTACCTTGGT ACAAATGAGA GGACACTTGG TGGAGACAGG 
AGGGAGTATG GTTTTGGGAA ATTGCGACAT GGAATTGCTT TTGGACAGAA ACCTCATCCC 
ACTTATGGCA TCAGGGGAGC TGTAGCACAG TTTGATGTTG TGAAAGCTTC TGGATTGATT 
CCTAAGAGGG GATGGGATGC AACTGAGACG CAAAAACTGG AGCTGACCAA AGGCAAATTG 
ATGATGGCTG ATACTTGTGT CAATGCTGAT GCTTTGATGG GGAGAATTGT AACAGCTGCT 
CACTCTGGGA AGAGGTTTTA TGTGGATTCC ATATGCTATG ATATGACTGC AGAGATCTCA 
TTTCCAAGAA AAGAGGGTTA TCTTGGTCCT CTGGAGTACA GCTCATATGC TGATTACTAC 
AAGCAAAAGT ATGGAGTTGA ATTGAAATTT AAGCAGCAGC CTTTACTAAG AGGACGTGGT 
GTTTCATACT GCAAGAATCT CTTATCTCCT CGGTTTGAAC ACTCAGATTC AAATGAAGGC 
GATGCTGAAG AGAATCTTGA CAAGACTTAC TATGTGTTTC TTCCTCCTGA GCTGTGTCTG 
GTGCACCCTC TTCCTGGATC ACTTGTTCGA GGTGCCCAGA GATTACCATC GATAATGAGG 
AGGGTTGAAA GCATGCTGCT CGCTGTTGAA CTTAAGGATA TAATAAATTA TCCAGTCCCT 
GCTTCAAAGA TCTTGGAAGC CTTGACTGCT GCTTCATGCC AAGAGACATT CTGCTATGAA 
AGAGCAGAGC TTCTAGGAGA TGCTTACCTG AAATGGGTGG TTAGTCGATT TCTTTTTCTG 
AAGTATCCCC AGAAACATGA GGGTCAACTT ACTAGAATGA GGCAACAAAT GGTAAGTAAT 
ATGGTTTTGT ATCAATATGC ACTTAACAAA GGACTTCAGT CATATATCCA AGCAGATCGC 
TTTGCTCCAT CTCGATGGGC TGCTCCTGGA GTGTTGCCTG TCTTTGATGA GGAAACAAAA 
GATGGAGACT CGTACATATT TGATCAGGAG AAGTCACTTG CTGAGGATAG AACCGGAATG 
AATCATCTTG ATGATGGGTA TGAAAATGAA ATTGAAGATG GTGAACTTGA GAGTGATGCT 
AGTTCTTACA GAGTCCTCTC TAGCAAGACA TTGGCAGATG TTGTTGAAGC ATTAATTGGT 
GTTTATTATG TTGAAGGTGG GAAAAATGCT GTTAACCACC TTATGAAATG GATTGGAATT 
CAGGTAGAGT TTGATCATGA AGAGATAGAT GGTGCATCTA GGCCATTCAA TGTTCCAGAA 
AGTGTTCTTA GGAGTGTTGA CTTTGATACT TTAGAAGGTG CCTTGGATAT TAAGTTCAAT 
GATCGTGGCT TGTTGATAGA AGCCATAACG CATGCTTCAC GACCATCTTC TGGAGTATCC 
TGCTATCAAC GATTAGAATT TGTTGGTGAC GCAGTCTTAG ATCATCTTAT TACTAGGCAC 
TTGTTTTTTA CATATACAAA TCTGCCCCCT GGTCGCTTGA CAGACTTGCG AGCCGCTGCT 
GTAAACAATG AAAATTTTGC ACGTGTTGCA GTTAAACATA AGCTTCATGT GCACCTTAGA 
CATGGGTCAA GTGCCCTTGA AAAGCAGATT CGGGACTTTG TTAGGGAAGT TCAAGATGAA 
CTATTGAAGC CAGTGTTCAA CTCCTTTGGT TTGGGAGATT GCAAAGCTCC AAAGGTTCTT 
GGTGACATCG TTGAATCCAT TGCTGGTGCT ATTTTTCTTG ACAGCGGACG AGATACAGCG 
GTTGTGTGGA AGGTTTTTCA ACCTCTGTTG CATCCAATGG TCACTCCAGA AACCCTGCCA 
ATGCATCCTG TAAGGGAGCT GCAGGAACGG TGCCAGCAAC AGGCTGAAGG ATTGGAATAC 
AAAGCCACTC GAAGTGGCAA TTTGGCTACT GTTGAAGTTT TCATTGATGG TGTTCAGGTA 
GGAGTTGCCC AAAATCCACA AAAGAAAATG GCACAGAAAC TCGCTGCTAG GAATGCACTT 
GTTGTTTTGA AAGAGAAGGA AACAGCTGAA GCCAAGGAAA AGAGTGATGA AAATGGGAAG 
AAGAAAAGGA ACGGCAACCA AACCTTTACT AGACAAACAT TAAATGATAT CTGCTTGCGT 
AGAAATTGGC CTATGCCATC ATATCGGTGT GTCAATGAGG GTGGTCCTGC ACATGCAAAG 
AGGTTTACTT TTGCAGTTCG TGTGAATACT ACCGATAGAG GATGGACAGA CGAATGTGTG 
GGAGAGCCGA TGCCGAGTGT TAAGAAGGCC AAAGACTCTG CTGCAGTTCT TCTCTTGGAA 
CTATTAAATA AACGGTACTC ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC18096402 ( XM_006386606.2 ) cDNA ORF clone, Populus trichocarpa(black cottonwood) -> XP_006386668.1 Populus trichocarpa endoribonuclease Dicer homolog 1 (LOC18096402), transcript variant X3, mRNA.

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CloneID	OPa07043
Clone ID Related Accession (Same CDS sequence)	XM_006386606.1 , XM_006386606.2
Accession Version	XM_006386606.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5904bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-04-08
Organism	Populus trichocarpa(black cottonwood)
Product	endoribonuclease Dicer homolog 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037286.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Apr 9, 2018 this sequence version replaced XM_006386606.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Populus trichocarpa Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGAGCG AGAGTAATGG TAGGGTTTCG GGGATTGGAG GTGGAGGTGG TCCGTCTTAC 
TGGCTTGATG CATGTGAGGA TATTTCTTGT GATATTATAG ATGATTTTGT TGATTTTGAT 
ACTTCAATTG TTCCAGAATT ATCTGTTGAT AATAATTCCA ATGTTAATAA TGATTTCTTT 
GGAGGGATTG ATCACATTCT TGATAGTATC AAGAATGGTA GTGGTCTTCC TCCTCTTCAT 
AATGCTAGTA CGACAGCTAA TGTTTCCAAT GGAAATCGGG ATTGCATTGT TGGTGATGGT 
TGGTTTATTA ATGTTGAAAA TGGAGTTTGC CATGGGAGCT CGGTTTCACA ATCAAATGGT 
GGTGATAAGG ACAATATTGA CAGAAAAGGG CAGGTGGAGA ATGGTGGTAA TGGGTTGAAT 
TTGTCCAATG GGAAGAGAGA AGAGAGGTTC TCAAACAATT TTGTTAAAGA AAATGGGAAG 
AAGGATGAGC AGTCGACGGA GCAAGGTATT GATGGTGATG AAAGGTGCGG TAAAAGGGCT 
AGACTTTGTT GTTATAGAAA TGAGAGAGTG TATTCTAGTA GAGGGGAGCA TAGGGATAGG 
GAGAGATGTT CTAGCAGGAA GAGGTCACGG GATTGGGATG AAAGCGATAG AAGGGATAGG 
GATATTAGTC GAAGAAGAGA TAGATATAGT GGTAGTAATA GGAGGGATGG TAGAGATAGG 
GATTGGAGGG AAAGAGAACT TAGGGGTTAT TGGGAGAGGG ATCGTTCAGG GTCCAAAGAC 
ATGGTTTTCC GATTGGGCAC TTGGGAAGCT GATCATAACA AAGAAGGAAG GGAGGCAAAT 
GACAAAATTC AGGAGTGCAA AGGAGAGCTA GAGAAGAAAT CAGAGGAGAG TAAAGAGAAA 
GTCCCTGAAG AACAGGCCCG CCAGTATCAA TTGGATGTTC TTGACCAGGC TAAGAAGAAA 
AATACAATAG CTTTTCTTGA AACAGGGGCA GGGAAGACAC TCATTGCTGT ACTCCTCATC 
AGAAGCATTT GCAATGACTT GCAAAGGCAG AATAAAAAAA TTCTAGCTGT ATTTTTAGTT 
CCGAAGGTTC CACTTGTTTA TCAGCAAGCA GAAGTTATTC GTGAGCGCGG TTACCAAGTG 
GGCCATTATT GTGGTGAGAT GGGTCAAGAT TTCTGGGATA CTCGAAGATG GCAGCGTGAG 
TTTGAAACAA AACAGGTTTT AGTAATGACA GCCCAAATTC TTTTGAACAT TCTGAGGCAC 
AGCATAATCA AAATGGAAGC CATAAATCTC CTCATTCTGG ATGAGTGTCA TCATGCTGTG 
AAGAAACATC CATATTCTCT TGTGATGTCT GAGTTTTACC ATACGACTCC CAAGGAGAAG 
AGACCGTCTG TTTTTGGAAT GACAGCTTCT CCTGTTAACT TAAAGGGTGT TTCAAGTCAG 
GTGGATTGTG CAATAAAGAT CCGCAATCTT GAAAGTAAAC TGGACTCAAT TGTTTGTACG 
ATAAAAGACC GCAAGGAACT GGAAAAGCAT GTACCAATGC CGGCAGAGGT TGTGGTGGAG 
TACGACAAAG CAGCTAGCTT GTGGTCCCTT CATGAACAAA TAAAGCAAAT CGAAGCAGCT 
GTTGAGGAAG CTGCACAGTC TAGCTCGAGA AGAAGTAAAT GGCAATTTAT GGGAGCTAGG 
GATGCTGGGG CCAAGGAAGA GCTGCGCCAG GTTTATGGTG TCTCTGAAAG AACAGAAAGT 
GATGGGGCTG CTAACTTAAT TCAAAAGTTG AGGGCAATTA ATTATGCACT TGGTGATCTG 
GGTCAGTGGT GTGCTTACAA GGTTGCACAA TCCTTTCTGA CTGCCTTACA AAATGACGAG 
AGAGCAAACT ATCAGCTTGA TGTTAAGTTT CAAGAATCCT ACCTCGAAAG AGTCGTGTTG 
CTCTTACAAT GCCAATTAAC AGAGGGAGCT GTTACAGACA AGGATACAAA AGTTTCAGAC 
AATGGGAATG ACAATATTCA GGATGGGCCT GGTTTTGATG AGATTGAGGA GGGAGAGCTC 
CCTGACAGTC ATGTTGTCTC TGGTGGAGAA CATGTTGATG TGATAATAGG AGCTGCTGTA 
GCTGATGGAA AAGTGACCCC TAAAGTGCAG TCACTGATTA AAGTACTTCT CAGATATCAG 
CACACGGAGG ATTTTCGTGC GATCATCTTT GTTGAGCGCG TAGTAGCTGC TTTGGTTCTT 
CCTAAGGTCT TTGCAGAGCT TCCATCTCTA AGTTTTGTTA GGTGTGCAAG TTTGATAGGG 
CACAATAATA GTCAGGAAAT GAGAACAAGC CAAATGCAAG ACACAATTGC CAAATTTCGA 
GATGGTCGTG TGACATTGTT AGTTGCTACC AGTGTTGCTG AGGAAGGACT TGATATTAGG 
CAATGCAATG TTGTTATTCG CTTTGATCTT GCAAAAACTG TTCTTGCATA TATTCAGTCT 
CGAGGTCGTG CAAGAAAGCC AGGCTCTGAT TACATCTTGA TGGTGGAGAG GGGAAACCTG 
TCTCATGGAG CATTCTTAAG GAATGCTAGG AATAGTGAGG AGACCCTGAG GAAAGAAGCG 
ATTGAGAGAA CTGACCTCAG TCATCTTAAG GATACTTCAA GGTTAATTGC AGTAGATTCA 
ATACCAGGAA CTGTGTATCA GGTGGAGTCT ACTGGTGCTG TTGTGAGCTT AAATTCTGCT 
GTTGGACTTG TTCATTTCTA TTGCTCTCAG TTACCCAGTG ACAGGTATTC AATACTTCGT 
CCTGGGTTTA TTATGGAGAA GCATGAGAAG CCAGGAGGTC CCACGGAATA TTCATGCAAG 
CTTCAGCTTC CTTGTAATGC ACCATTTGAG GAGCTTGAGG GTCCTGTATG CAGTTCAATG 
CGCCTTGCAC ATCAGGCTGT TTGTTTGGCT GCTTGCAAGA AGCTTCATGA GATGGGAGCA 
TTTACTGACA TGCTCTTGCC AGACAAGGGA AGTGAGGAAG AAAAGGACAA GGTTGACCAG 
AATGATGAAG GTGAACCACT TCCTGGTACT GCTAGGCATC GGGAGTTCTA TCCTGAAGGT 
GTAGCTAAGA CACTCCAGGG AGAATGGATC TTATGCGGAA GGGATGGTTG CAACAACTCC 
AAAGTGCTTC ATCTCTACTT GTATGGAGTC AGATGCTTAA ACATTGGCAC TTCAAATGAT 
CCATTCTTGA CTCAAGTTTC AAATTTTGCA GTGCTTTTTG GCAATGAGCT GGATGCAGAG 
GTCTTATCGA TGTCGATGGA TCTATTTATC GCTCGAACCA TGATAACGAA GGCATCTCTT 
GTCTTTAGGG GTCGCATACC TATTACAGAA AGTCAGCTGG CATCCCTTAA AAATTTTCAT 
GTAAGGTTGA TGAGCATTGT ACTGGATGTG GATGTTGAAC CTTCCACCAC TCCATGGGAT 
CCTGCAAAAG CTTATTTATT CGTCCCTATG GTTAGCGATA AGTCTGTAGA TCCTATAAAA 
GAAATTGACT GGGATCTGGT TGAAAATATA ATTGGAACAG ATGCTTGGAG CAATCGTCTT 
CAGAGAGCTC GACCAGATGT TTACCTTGGT ACAAATGAGA GGACACTTGG TGGAGACAGG 
AGGGAGTATG GTTTTGGGAA ATTGCGACAT GGAATTGCTT TTGGACAGAA ACCTCATCCC 
ACTTATGGCA TCAGGGGAGC TGTAGCACAG TTTGATGTTG TGAAAGCTTC TGGATTGATT 
CCTAAGAGGG GATGGGATGC AACTGAGACG CAAAAACTGG AGCTGACCAA AGGCAAATTG 
ATGATGGCTG ATACTTGTGT CAATGCTGAT GCTTTGATGG GGAGAATTGT AACAGCTGCT 
CACTCTGGGA AGAGGTTTTA TGTGGATTCC ATATGCTATG ATATGACTGC AGAGATCTCA 
TTTCCAAGAA AAGAGGGTTA TCTTGGTCCT CTGGAGTACA GCTCATATGC TGATTACTAC 
AAGCAAAAGT ATGGAGTTGA ATTGAAATTT AAGCAGCAGC CTTTACTAAG AGGACGTGGT 
GTTTCATACT GCAAGAATCT CTTATCTCCT CGGTTTGAAC ACTCAGATTC AAATGAAGGC 
GATGCTGAAG AGAATCTTGA CAAGACTTAC TATGTGTTTC TTCCTCCTGA GCTGTGTCTG 
GTGCACCCTC TTCCTGGATC ACTTGTTCGA GGTGCCCAGA GATTACCATC GATAATGAGG 
AGGGTTGAAA GCATGCTGCT CGCTGTTGAA CTTAAGGATA TAATAAATTA TCCAGTCCCT 
GCTTCAAAGA TCTTGGAAGC CTTGACTGCT GCTTCATGCC AAGAGACATT CTGCTATGAA 
AGAGCAGAGC TTCTAGGAGA TGCTTACCTG AAATGGGTGG TTAGTCGATT TCTTTTTCTG 
AAGTATCCCC AGAAACATGA GGGTCAACTT ACTAGAATGA GGCAACAAAT GGTAAGTAAT 
ATGGTTTTGT ATCAATATGC ACTTAACAAA GGACTTCAGT CATATATCCA AGCAGATCGC 
TTTGCTCCAT CTCGATGGGC TGCTCCTGGA GTGTTGCCTG TCTTTGATGA GGAAACAAAA 
GATGGAGACT CGTACATATT TGATCAGGAG AAGTCACTTG CTGAGGATAG AACCGGAATG 
AATCATCTTG ATGATGGGTA TGAAAATGAA ATTGAAGATG GTGAACTTGA GAGTGATGCT 
AGTTCTTACA GAGTCCTCTC TAGCAAGACA TTGGCAGATG TTGTTGAAGC ATTAATTGGT 
GTTTATTATG TTGAAGGTGG GAAAAATGCT GTTAACCACC TTATGAAATG GATTGGAATT 
CAGGTAGAGT TTGATCATGA AGAGATAGAT GGTGCATCTA GGCCATTCAA TGTTCCAGAA 
AGTGTTCTTA GGAGTGTTGA CTTTGATACT TTAGAAGGTG CCTTGGATAT TAAGTTCAAT 
GATCGTGGCT TGTTGATAGA AGCCATAACG CATGCTTCAC GACCATCTTC TGGAGTATCC 
TGCTATCAAC GATTAGAATT TGTTGGTGAC GCAGTCTTAG ATCATCTTAT TACTAGGCAC 
TTGTTTTTTA CATATACAAA TCTGCCCCCT GGTCGCTTGA CAGACTTGCG AGCCGCTGCT 
GTAAACAATG AAAATTTTGC ACGTGTTGCA GTTAAACATA AGCTTCATGT GCACCTTAGA 
CATGGGTCAA GTGCCCTTGA AAAGCAGATT CGGGACTTTG TTAGGGAAGT TCAAGATGAA 
CTATTGAAGC CAGTGTTCAA CTCCTTTGGT TTGGGAGATT GCAAAGCTCC AAAGGTTCTT 
GGTGACATCG TTGAATCCAT TGCTGGTGCT ATTTTTCTTG ACAGCGGACG AGATACAGCG 
GTTGTGTGGA AGGTTTTTCA ACCTCTGTTG CATCCAATGG TCACTCCAGA AACCCTGCCA 
ATGCATCCTG TAAGGGAGCT GCAGGAACGG TGCCAGCAAC AGGCTGAAGG ATTGGAATAC 
AAAGCCACTC GAAGTGGCAA TTTGGCTACT GTTGAAGTTT TCATTGATGG TGTTCAGGTA 
GGAGTTGCCC AAAATCCACA AAAGAAAATG GCACAGAAAC TCGCTGCTAG GAATGCACTT 
GTTGTTTTGA AAGAGAAGGA AACAGCTGAA GCCAAGGAAA AGAGTGATGA AAATGGGAAG 
AAGAAAAGGA ACGGCAACCA AACCTTTACT AGACAAACAT TAAATGATAT CTGCTTGCGT 
AGAAATTGGC CTATGCCATC ATATCGGTGT GTCAATGAGG GTGGTCCTGC ACATGCAAAG 
AGGTTTACTT TTGCAGTTCG TGTGAATACT ACCGATAGAG GATGGACAGA CGAATGTGTG 
GGAGAGCCGA TGCCGAGTGT TAAGAAGGCC AAAGACTCTG CTGCAGTTCT TCTCTTGGAA 
CTATTAAATA AACGGTACTC ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006386668.1
CDS	924..6827
Translation	MESESNGRVSGIGGGGGPSYWLDACEDISCDIIDDFVDFDTSIVPELSVDNNSNVNNDFFGGIDHILDSIKNGSGLPPLHNASTTANVSNGNRDCIVGDGWFINVENGVCHGSSVSQSNGGDKDNIDRKGQVENGGNGLNLSNGKREERFSNNFVKENGKKDEQSTEQGIDGDERCGKRARLCCYRNERVYSSRGEHRDRERCSSRKRSRDWDESDRRDRDISRRRDRYSGSNRRDGRDRDWRERELRGYWERDRSGSKDMVFRLGTWEADHNKEGREANDKIQECKGELEKKSEESKEKVPEEQARQYQLDVLDQAKKKNTIAFLETGAGKTLIAVLLIRSICNDLQRQNKKILAVFLVPKVPLVYQQAEVIRERGYQVGHYCGEMGQDFWDTRRWQREFETKQVLVMTAQILLNILRHSIIKMEAINLLILDECHHAVKKHPYSLVMSEFYHTTPKEKRPSVFGMTASPVNLKGVSSQVDCAIKIRNLESKLDSIVCTIKDRKELEKHVPMPAEVVVEYDKAASLWSLHEQIKQIEAAVEEAAQSSSRRSKWQFMGARDAGAKEELRQVYGVSERTESDGAANLIQKLRAINYALGDLGQWCAYKVAQSFLTALQNDERANYQLDVKFQESYLERVVLLLQCQLTEGAVTDKDTKVSDNGNDNIQDGPGFDEIEEGELPDSHVVSGGEHVDVIIGAAVADGKVTPKVQSLIKVLLRYQHTEDFRAIIFVERVVAALVLPKVFAELPSLSFVRCASLIGHNNSQEMRTSQMQDTIAKFRDGRVTLLVATSVAEEGLDIRQCNVVIRFDLAKTVLAYIQSRGRARKPGSDYILMVERGNLSHGAFLRNARNSEETLRKEAIERTDLSHLKDTSRLIAVDSIPGTVYQVESTGAVVSLNSAVGLVHFYCSQLPSDRYSILRPGFIMEKHEKPGGPTEYSCKLQLPCNAPFEELEGPVCSSMRLAHQAVCLAACKKLHEMGAFTDMLLPDKGSEEEKDKVDQNDEGEPLPGTARHREFYPEGVAKTLQGEWILCGRDGCNNSKVLHLYLYGVRCLNIGTSNDPFLTQVSNFAVLFGNELDAEVLSMSMDLFIARTMITKASLVFRGRIPITESQLASLKNFHVRLMSIVLDVDVEPSTTPWDPAKAYLFVPMVSDKSVDPIKEIDWDLVENIIGTDAWSNRLQRARPDVYLGTNERTLGGDRREYGFGKLRHGIAFGQKPHPTYGIRGAVAQFDVVKASGLIPKRGWDATETQKLELTKGKLMMADTCVNADALMGRIVTAAHSGKRFYVDSICYDMTAEISFPRKEGYLGPLEYSSYADYYKQKYGVELKFKQQPLLRGRGVSYCKNLLSPRFEHSDSNEGDAEENLDKTYYVFLPPELCLVHPLPGSLVRGAQRLPSIMRRVESMLLAVELKDIINYPVPASKILEALTAASCQETFCYERAELLGDAYLKWVVSRFLFLKYPQKHEGQLTRMRQQMVSNMVLYQYALNKGLQSYIQADRFAPSRWAAPGVLPVFDEETKDGDSYIFDQEKSLAEDRTGMNHLDDGYENEIEDGELESDASSYRVLSSKTLADVVEALIGVYYVEGGKNAVNHLMKWIGIQVEFDHEEIDGASRPFNVPESVLRSVDFDTLEGALDIKFNDRGLLIEAITHASRPSSGVSCYQRLEFVGDAVLDHLITRHLFFTYTNLPPGRLTDLRAAAVNNENFARVAVKHKLHVHLRHGSSALEKQIRDFVREVQDELLKPVFNSFGLGDCKAPKVLGDIVESIAGAIFLDSGRDTAVVWKVFQPLLHPMVTPETLPMHPVRELQERCQQQAEGLEYKATRSGNLATVEVFIDGVQVGVAQNPQKKMAQKLAARNALVVLKEKETAEAKEKSDENGKKKRNGNQTFTRQTLNDICLRRNWPMPSYRCVNEGGPAHAKRFTFAVRVNTTDRGWTDECVGEPMPSVKKAKDSAAVLLLELLNKRYS

Target ORF information:

RefSeq Version	XM_006386606.2
Organism	Populus trichocarpa(black cottonwood)
Definition	Populus trichocarpa endoribonuclease Dicer homolog 1 (LOC18096402), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006386606.2

ORF Insert Sequence:

ATGGAGAGCG AGAGTAATGG TAGGGTTTCG GGGATTGGAG GTGGAGGTGG TCCGTCTTAC 
TGGCTTGATG CATGTGAGGA TATTTCTTGT GATATTATAG ATGATTTTGT TGATTTTGAT 
ACTTCAATTG TTCCAGAATT ATCTGTTGAT AATAATTCCA ATGTTAATAA TGATTTCTTT 
GGAGGGATTG ATCACATTCT TGATAGTATC AAGAATGGTA GTGGTCTTCC TCCTCTTCAT 
AATGCTAGTA CGACAGCTAA TGTTTCCAAT GGAAATCGGG ATTGCATTGT TGGTGATGGT 
TGGTTTATTA ATGTTGAAAA TGGAGTTTGC CATGGGAGCT CGGTTTCACA ATCAAATGGT 
GGTGATAAGG ACAATATTGA CAGAAAAGGG CAGGTGGAGA ATGGTGGTAA TGGGTTGAAT 
TTGTCCAATG GGAAGAGAGA AGAGAGGTTC TCAAACAATT TTGTTAAAGA AAATGGGAAG 
AAGGATGAGC AGTCGACGGA GCAAGGTATT GATGGTGATG AAAGGTGCGG TAAAAGGGCT 
AGACTTTGTT GTTATAGAAA TGAGAGAGTG TATTCTAGTA GAGGGGAGCA TAGGGATAGG 
GAGAGATGTT CTAGCAGGAA GAGGTCACGG GATTGGGATG AAAGCGATAG AAGGGATAGG 
GATATTAGTC GAAGAAGAGA TAGATATAGT GGTAGTAATA GGAGGGATGG TAGAGATAGG 
GATTGGAGGG AAAGAGAACT TAGGGGTTAT TGGGAGAGGG ATCGTTCAGG GTCCAAAGAC 
ATGGTTTTCC GATTGGGCAC TTGGGAAGCT GATCATAACA AAGAAGGAAG GGAGGCAAAT 
GACAAAATTC AGGAGTGCAA AGGAGAGCTA GAGAAGAAAT CAGAGGAGAG TAAAGAGAAA 
GTCCCTGAAG AACAGGCCCG CCAGTATCAA TTGGATGTTC TTGACCAGGC TAAGAAGAAA 
AATACAATAG CTTTTCTTGA AACAGGGGCA GGGAAGACAC TCATTGCTGT ACTCCTCATC 
AGAAGCATTT GCAATGACTT GCAAAGGCAG AATAAAAAAA TTCTAGCTGT ATTTTTAGTT 
CCGAAGGTTC CACTTGTTTA TCAGCAAGCA GAAGTTATTC GTGAGCGCGG TTACCAAGTG 
GGCCATTATT GTGGTGAGAT GGGTCAAGAT TTCTGGGATA CTCGAAGATG GCAGCGTGAG 
TTTGAAACAA AACAGGTTTT AGTAATGACA GCCCAAATTC TTTTGAACAT TCTGAGGCAC 
AGCATAATCA AAATGGAAGC CATAAATCTC CTCATTCTGG ATGAGTGTCA TCATGCTGTG 
AAGAAACATC CATATTCTCT TGTGATGTCT GAGTTTTACC ATACGACTCC CAAGGAGAAG 
AGACCGTCTG TTTTTGGAAT GACAGCTTCT CCTGTTAACT TAAAGGGTGT TTCAAGTCAG 
GTGGATTGTG CAATAAAGAT CCGCAATCTT GAAAGTAAAC TGGACTCAAT TGTTTGTACG 
ATAAAAGACC GCAAGGAACT GGAAAAGCAT GTACCAATGC CGGCAGAGGT TGTGGTGGAG 
TACGACAAAG CAGCTAGCTT GTGGTCCCTT CATGAACAAA TAAAGCAAAT CGAAGCAGCT 
GTTGAGGAAG CTGCACAGTC TAGCTCGAGA AGAAGTAAAT GGCAATTTAT GGGAGCTAGG 
GATGCTGGGG CCAAGGAAGA GCTGCGCCAG GTTTATGGTG TCTCTGAAAG AACAGAAAGT 
GATGGGGCTG CTAACTTAAT TCAAAAGTTG AGGGCAATTA ATTATGCACT TGGTGATCTG 
GGTCAGTGGT GTGCTTACAA GGTTGCACAA TCCTTTCTGA CTGCCTTACA AAATGACGAG 
AGAGCAAACT ATCAGCTTGA TGTTAAGTTT CAAGAATCCT ACCTCGAAAG AGTCGTGTTG 
CTCTTACAAT GCCAATTAAC AGAGGGAGCT GTTACAGACA AGGATACAAA AGTTTCAGAC 
AATGGGAATG ACAATATTCA GGATGGGCCT GGTTTTGATG AGATTGAGGA GGGAGAGCTC 
CCTGACAGTC ATGTTGTCTC TGGTGGAGAA CATGTTGATG TGATAATAGG AGCTGCTGTA 
GCTGATGGAA AAGTGACCCC TAAAGTGCAG TCACTGATTA AAGTACTTCT CAGATATCAG 
CACACGGAGG ATTTTCGTGC GATCATCTTT GTTGAGCGCG TAGTAGCTGC TTTGGTTCTT 
CCTAAGGTCT TTGCAGAGCT TCCATCTCTA AGTTTTGTTA GGTGTGCAAG TTTGATAGGG 
CACAATAATA GTCAGGAAAT GAGAACAAGC CAAATGCAAG ACACAATTGC CAAATTTCGA 
GATGGTCGTG TGACATTGTT AGTTGCTACC AGTGTTGCTG AGGAAGGACT TGATATTAGG 
CAATGCAATG TTGTTATTCG CTTTGATCTT GCAAAAACTG TTCTTGCATA TATTCAGTCT 
CGAGGTCGTG CAAGAAAGCC AGGCTCTGAT TACATCTTGA TGGTGGAGAG GGGAAACCTG 
TCTCATGGAG CATTCTTAAG GAATGCTAGG AATAGTGAGG AGACCCTGAG GAAAGAAGCG 
ATTGAGAGAA CTGACCTCAG TCATCTTAAG GATACTTCAA GGTTAATTGC AGTAGATTCA 
ATACCAGGAA CTGTGTATCA GGTGGAGTCT ACTGGTGCTG TTGTGAGCTT AAATTCTGCT 
GTTGGACTTG TTCATTTCTA TTGCTCTCAG TTACCCAGTG ACAGGTATTC AATACTTCGT 
CCTGGGTTTA TTATGGAGAA GCATGAGAAG CCAGGAGGTC CCACGGAATA TTCATGCAAG 
CTTCAGCTTC CTTGTAATGC ACCATTTGAG GAGCTTGAGG GTCCTGTATG CAGTTCAATG 
CGCCTTGCAC ATCAGGCTGT TTGTTTGGCT GCTTGCAAGA AGCTTCATGA GATGGGAGCA 
TTTACTGACA TGCTCTTGCC AGACAAGGGA AGTGAGGAAG AAAAGGACAA GGTTGACCAG 
AATGATGAAG GTGAACCACT TCCTGGTACT GCTAGGCATC GGGAGTTCTA TCCTGAAGGT 
GTAGCTAAGA CACTCCAGGG AGAATGGATC TTATGCGGAA GGGATGGTTG CAACAACTCC 
AAAGTGCTTC ATCTCTACTT GTATGGAGTC AGATGCTTAA ACATTGGCAC TTCAAATGAT 
CCATTCTTGA CTCAAGTTTC AAATTTTGCA GTGCTTTTTG GCAATGAGCT GGATGCAGAG 
GTCTTATCGA TGTCGATGGA TCTATTTATC GCTCGAACCA TGATAACGAA GGCATCTCTT 
GTCTTTAGGG GTCGCATACC TATTACAGAA AGTCAGCTGG CATCCCTTAA AAATTTTCAT 
GTAAGGTTGA TGAGCATTGT ACTGGATGTG GATGTTGAAC CTTCCACCAC TCCATGGGAT 
CCTGCAAAAG CTTATTTATT CGTCCCTATG GTTAGCGATA AGTCTGTAGA TCCTATAAAA 
GAAATTGACT GGGATCTGGT TGAAAATATA ATTGGAACAG ATGCTTGGAG CAATCGTCTT 
CAGAGAGCTC GACCAGATGT TTACCTTGGT ACAAATGAGA GGACACTTGG TGGAGACAGG 
AGGGAGTATG GTTTTGGGAA ATTGCGACAT GGAATTGCTT TTGGACAGAA ACCTCATCCC 
ACTTATGGCA TCAGGGGAGC TGTAGCACAG TTTGATGTTG TGAAAGCTTC TGGATTGATT 
CCTAAGAGGG GATGGGATGC AACTGAGACG CAAAAACTGG AGCTGACCAA AGGCAAATTG 
ATGATGGCTG ATACTTGTGT CAATGCTGAT GCTTTGATGG GGAGAATTGT AACAGCTGCT 
CACTCTGGGA AGAGGTTTTA TGTGGATTCC ATATGCTATG ATATGACTGC AGAGATCTCA 
TTTCCAAGAA AAGAGGGTTA TCTTGGTCCT CTGGAGTACA GCTCATATGC TGATTACTAC 
AAGCAAAAGT ATGGAGTTGA ATTGAAATTT AAGCAGCAGC CTTTACTAAG AGGACGTGGT 
GTTTCATACT GCAAGAATCT CTTATCTCCT CGGTTTGAAC ACTCAGATTC AAATGAAGGC 
GATGCTGAAG AGAATCTTGA CAAGACTTAC TATGTGTTTC TTCCTCCTGA GCTGTGTCTG 
GTGCACCCTC TTCCTGGATC ACTTGTTCGA GGTGCCCAGA GATTACCATC GATAATGAGG 
AGGGTTGAAA GCATGCTGCT CGCTGTTGAA CTTAAGGATA TAATAAATTA TCCAGTCCCT 
GCTTCAAAGA TCTTGGAAGC CTTGACTGCT GCTTCATGCC AAGAGACATT CTGCTATGAA 
AGAGCAGAGC TTCTAGGAGA TGCTTACCTG AAATGGGTGG TTAGTCGATT TCTTTTTCTG 
AAGTATCCCC AGAAACATGA GGGTCAACTT ACTAGAATGA GGCAACAAAT GGTAAGTAAT 
ATGGTTTTGT ATCAATATGC ACTTAACAAA GGACTTCAGT CATATATCCA AGCAGATCGC 
TTTGCTCCAT CTCGATGGGC TGCTCCTGGA GTGTTGCCTG TCTTTGATGA GGAAACAAAA 
GATGGAGACT CGTACATATT TGATCAGGAG AAGTCACTTG CTGAGGATAG AACCGGAATG 
AATCATCTTG ATGATGGGTA TGAAAATGAA ATTGAAGATG GTGAACTTGA GAGTGATGCT 
AGTTCTTACA GAGTCCTCTC TAGCAAGACA TTGGCAGATG TTGTTGAAGC ATTAATTGGT 
GTTTATTATG TTGAAGGTGG GAAAAATGCT GTTAACCACC TTATGAAATG GATTGGAATT 
CAGGTAGAGT TTGATCATGA AGAGATAGAT GGTGCATCTA GGCCATTCAA TGTTCCAGAA 
AGTGTTCTTA GGAGTGTTGA CTTTGATACT TTAGAAGGTG CCTTGGATAT TAAGTTCAAT 
GATCGTGGCT TGTTGATAGA AGCCATAACG CATGCTTCAC GACCATCTTC TGGAGTATCC 
TGCTATCAAC GATTAGAATT TGTTGGTGAC GCAGTCTTAG ATCATCTTAT TACTAGGCAC 
TTGTTTTTTA CATATACAAA TCTGCCCCCT GGTCGCTTGA CAGACTTGCG AGCCGCTGCT 
GTAAACAATG AAAATTTTGC ACGTGTTGCA GTTAAACATA AGCTTCATGT GCACCTTAGA 
CATGGGTCAA GTGCCCTTGA AAAGCAGATT CGGGACTTTG TTAGGGAAGT TCAAGATGAA 
CTATTGAAGC CAGTGTTCAA CTCCTTTGGT TTGGGAGATT GCAAAGCTCC AAAGGTTCTT 
GGTGACATCG TTGAATCCAT TGCTGGTGCT ATTTTTCTTG ACAGCGGACG AGATACAGCG 
GTTGTGTGGA AGGTTTTTCA ACCTCTGTTG CATCCAATGG TCACTCCAGA AACCCTGCCA 
ATGCATCCTG TAAGGGAGCT GCAGGAACGG TGCCAGCAAC AGGCTGAAGG ATTGGAATAC 
AAAGCCACTC GAAGTGGCAA TTTGGCTACT GTTGAAGTTT TCATTGATGG TGTTCAGGTA 
GGAGTTGCCC AAAATCCACA AAAGAAAATG GCACAGAAAC TCGCTGCTAG GAATGCACTT 
GTTGTTTTGA AAGAGAAGGA AACAGCTGAA GCCAAGGAAA AGAGTGATGA AAATGGGAAG 
AAGAAAAGGA ACGGCAACCA AACCTTTACT AGACAAACAT TAAATGATAT CTGCTTGCGT 
AGAAATTGGC CTATGCCATC ATATCGGTGT GTCAATGAGG GTGGTCCTGC ACATGCAAAG 
AGGTTTACTT TTGCAGTTCG TGTGAATACT ACCGATAGAG GATGGACAGA CGAATGTGTG 
GGAGAGCCGA TGCCGAGTGT TAAGAAGGCC AAAGACTCTG CTGCAGTTCT TCTCTTGGAA 
CTATTAAATA AACGGTACTC ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The genome of black cottonwood, Populus trichocarpa (Torr. & Gray).
Science (New York, N.Y.)313(5793)1596-604(2006 Sep)
Tuskan GA,Difazio S,Jansson S,Bohlmann J,Grigoriev I,Hellsten U,Putnam N,Ralph S,Rombauts S,Salamov A,Schein J,Sterck L,Aerts A,Bhalerao RR,Bhalerao RP,Blaudez D,Boerjan W,Brun A,Brunner A,Busov V,Campbell M,Carlson J,Chalot M,Chapman J,Chen GL,Cooper D,Coutinho PM,Couturier J,Covert S,Cronk Q,Cunningham R,Davis J,Degroeve S,D?jardin A,Depamphilis C,Detter J,Dirks B,Dubchak I,Duplessis S,Ehlting J,Ellis B,Gendler K,Goodstein D,Gribskov M,Grimwood J,Groover A,Gunter L,Hamberger B,Heinze B,Helariutta Y,Henrissat B,Holligan D,Holt R,Huang W,Islam-Faridi N,Jones S,Jones-Rhoades M,Jorgensen R,Joshi C,Kangasj?rvi J,Karlsson J,Kelleher C,Kirkpatrick R,Kirst M,Kohler A,Kalluri U,Larimer F,Leebens-Mack J,Lepl? JC,Locascio P,Lou Y,Lucas S,Martin F,Montanini B,Napoli C,Nelson DR,Nelson C,Nieminen K,Nilsson O,Pereda V,Peter G,Philippe R,Pilate G,Poliakov A,Razumovskaya J,Richardson P,Rinaldi C,Ritland K,Rouz? P,Ryaboy D,Schmutz J,Schrader J,Segerman B,Shin H,Siddiqui A,Sterky F,Terry A,Tsai CJ,Uberbacher E,Unneberg P,Vahala J,Wall K,Wessler S,Yang G,Yin T,Douglas C,Marra M,Sandberg G,Van de Peer Y,Rokhsar D

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LOC18096402 cDNA ORF clone, Populus trichocarpa(black cottonwood)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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