LOC105132845 cDNA ORF clone, Populus euphratica(Euphrates poplar)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC105132845
Entrez Gene ID	105132845
Full Name	Niemann-Pick C1 protein-like
Gene Type	protein-coding
Organism	Populus euphratica(Euphrates poplar)

mRNA and Protein(s)

mRNA	Protein	Name
XM_011036567.1	XP_011034869.1	Niemann-Pick C1 protein-like

Pathways from BioSystems
Gene Ontology

The following LOC105132845 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC105132845 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OPo277325	XM_011036567.1 Latest version!	Populus euphratica Niemann-Pick C1 protein-like (LOC105132845), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC105132845 ( XM_011036567.1 ) cDNA ORF clone, Populus euphratica(Euphrates poplar) -> XP_011034869.1 Populus euphratica Niemann-Pick C1 protein-like (LOC105132845), mRNA.

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CloneID	OPo277325
Clone ID Related Accession (Same CDS sequence)	XM_011036567.1
Accession Version	XM_011036567.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3885bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-01-05
Organism	Populus euphratica(Euphrates poplar)
Product	Niemann-Pick C1 protein-like
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011499901.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Populus euphratica Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCTCT CAAGCAGGAC GATGAAGCTT TTTCTGCTTT CGATTTTTCT ATTACAGGTG 
TTTTATGCAG TATCTATAGT CAGCGCGGAG AGGTCTGATG CACGGTCGCT TAAGACACGA 
AATGCAGTAT CAGGGGAGAT ACATTCACAG GAATATTGTG CAATGTATGA TATTTGTGGT 
GCACGTGAAG ATGGAAAAGT CGTGAACTGT CCTTTTAGCT CCCCATCTGT GAAGCCAGAT 
GACTTGCTTT CACAAAAGAT TCAAAGCCTA TGCCCTACAA TCACTGGCAA TGTTTGTTGT 
TCAGAAGCTC AGTTTGAAAC TTTACGGTCA CAAGTCCAGC AAGCAATTCC ATTTCTGGTC 
GGCTGTCCTG CATGTTTGAG AAACTTTTTG AATTTGTTCT GTGAGCTTAC CTGTTCCCCG 
CATCAGAGCA TGTTTATTAA CGTGACTTCT ACGGATAAGG TTAAAGGCAA TTTGACCGTG 
AGTGGCATTG ATTTTTATGT ATATGATTCT TTTGGTGAAG GGTTGTATGA ATCTTGCAAG 
GATGTAAAGT TTGGGACCAT GAATAGTCGG GCTCTAAATT TTATTGGTGC TGGTGCTAAA 
AATTTCACAG AGTGGTATGC ATTTATTGGT AGACGAGCAC CCCTTAATGT GCCGGGCTCA 
CCATATGCTA TGACATTCAA GCCTTCTGCT CCTGAGTCAT CTGGAATGAA ACCCATGAAT 
GTTTCTACAT ATTCATGTGG TGATATTTCA CTGGGTTGTT CTTGTGGTGA TTGTCCTCAA 
TCTCCTGTTT GTGCAAATAC TGATCCTCCT CCACATCACG AGGGGGCTTC TTGTGCGGTG 
AGAATTGGAT CTCTCAAGGC CAAATGTGTC GACTTTATTC TAACAATCCT CTATGTTATA 
TTGGTTTCCA TCTTTTTGGG ATGGGGCTTG TTTCATCGAA AAAGAGAAAG GGACCAGAGT 
TCTAGAATGA ATCCAGTGTC GAATATCAAG GACAGTGGTG AAGTTACTGG GAAGAAGGAT 
GAGAACGTTA CCATGCAGAT GCTTGAAGAT TCTCCTCAAA CCGGAAGCAG GGTTCAGCTT 
TCAATTGTTC AAGGATACAT GTCGAAGTTC TACAGGTGTT ATGGAACATG GGTTGCTAGA 
AACCCAATCC TTGTGTTGAT CTTGTCGTTG GCTGTAATTC TTTTACTTTG TCTAGGTCTC 
ATCCGTTTCA AGGTGGAGAC ACGGCCTGAG AAGCTATGGG TAGGGCCTGG GAGTAAAGTT 
GCGGAAGAGA AAAGGTTTTT TGACACCCAC CTAGCCCCCT TTTACAGAAT TGAGCAGCTA 
ATTCTAGCGA CTGTTCCAGA AGCTGGGGCA CAGAAAAGAC CAAGTATTGT GACAGAAAAC 
AACATCAAAT TACTTTTTGA AATACAAAAA AAGGTTGATG GAATCCATGC AAATCATTCT 
GGAACAATGA TATCTCTGAC TGATATTTGC TTGAAGCCAC TGGACAAAGA TTGTGCTACT 
CAGAGTTTAC TGCAGTATTT CCAAATGGAT CCTCAAAATC TCGATAATTA TGGAGGAGTT 
GAGCACGTGA ACTATTGTCT CCAGCATTAT TCCTCTGCAG ACACATGTCG GAGTGCCTTC 
AAAGCTCCCC TTGATCCAAG CACAGCCTTG GGAGGTTTCT CTGGGAACAA TTATTCGGAG 
GCATCTGCAT TTATTGTAAC TTATCCAGTA AATAATGTGA TTGATAAAGA AGGGAATGAA 
ACTGATAAGG TGGTTGCTTG GGAGAAGGCT TTTATTCAGT TAGTGAAGAA TGAATTATTG 
CCAATGGTGC AATCTAAGAA TCTCACTCTT TCTTTTTCTT CGGAAAGCTC CATTGAGGAG 
GAACTAAAAA GAGAAAGCAC TGCTGATGTC ATAACTATAT TGATTAGCTA TCTTGTGATG 
TTTGCCTACA TCTCTCTTAC TTTGGGTGAC ACTCCTCATT TATCTTCGTT TTACATTTCA 
TCTAAGGTAT TGCTTGGTCT TTCTGGAGTT ATGCTTGTCA TGTTGTCTGT TCTTGGATCT 
GTTGGATTTT TTAGTGCCAT TGGAGTGAAG TCTACACTAA TCATCATGGA AGTCATTCCT 
TTTCTTGTAT TAGCTGTTGG GGTGGATAAC ATGTGCATAT TGGTGCATGC TGTCAAGAGG 
CAACCAATGG AGTTGCCGCT AGAGGGACGG ATAAGCAATG CTCTTGTGGA AGTTGGACCA 
TCCATCACAC TTGCTAGTCT ATCCGAGGTT TTAGCATTTG CAGTTGGAAG TTTCATTCCT 
ATGCCAGCAT GCCGTGTGTT CTCTATGTTC GCTGCACTTG CTGTTCTTTT GGACTTTCTT 
TTGCAAGTTA CTGCCTTTGT AGCTTTCATA GTTTTTGATT TTTTGCGAGC TGAGGATAAG 
AGAATTGATT GCTTTCCATG TCGGAAAATC TCATCTTCAT CTGCAGACTC TGATAAAGGT 
ATTGGTGGGA GAAGACCTGG ATTGCTGGCT CGCTATATGA AGGAGATCCA CGCACCCATA 
CTCAGTCTTT GGGGAGTTAA GATATTTGTC ATTGCCATCT TTGCTGCTTT CACATTGTCC 
AGCATAGCAT TATCCACTCG AGTTCAACCT GGTTTAGAAC AAAAAATTGT TCTTCCGCGA 
GACTCATATC TTCAGGGCTA TTTTAATAAT GTCTCAGAGT ATCTCAGAAT TGGTCCGCCA 
CTATATTTTG TAGTGAAGAA CTACAACTAT AGCTCAGAAT CAAGTCAGAC AAATCAGCTG 
TGCTCCATCA GCCACTGCAA TTCAAACTCT CTTTTAAATG AGATTGCAAG AGCATCCTTA 
ACACCAGAAT CTAGCTATAT AGCTATGCCA GCTGCTTCAT GGCTTGATGA TTTTCTTGTA 
TGGATATCTC CTGAAGCTTT TGGCTGCTGT AGGAAGTTCA CAAATGGGAC TTATTGCCCC 
CCTGATGATC AGTCTCCTTG TTGTTCATCT GATACAGGTT CATGTGGTCT TGGTGGGATA 
TGCAAAGATT GTACTACTTG CTTCCGTCAC TCAGATTTGA ATAATGATCG TCCATCTACA 
TCTCAGTTCA AGGAGAAACT CCCATGGTTC CTCAATGCTT TGCCCTCTGC TGACTGTGCC 
AAAGGCGGCC ATGGTGCTTA CACTAGCAGT ATAGATCTAC AAGGCTATGA GAATGGTGTT 
ATTGAAGCAT CATCCTTCCG TACATATCAT ACACCTCTTA ACAAGCAGAT TGACTATGTC 
AATTCAATGA GGGCTGCTCG AGAGTTCAGT TCAAGGGCTT CTGATTCTTT GAAGATGGAG 
ATCTTCCCAT ACTCTGTATT TTATATGTTT TTTGAACAAT ACCTTGATAT ATGGAGGACT 
GCATTGATCA ACCTCGCCAT AGCAATTGGA GCGGTATTTG TTGTTTGCTT GGTCATCACT 
TGCAGTTTGT GGAGTTCTGC TATCATTTTG CTGGTGTTGG TAATGATAGT CGTGGATCTC 
ATGGGCGTGA TGGCAATTCT GAACATTCAA CTGAATGCAG TCTCTGTTGT CAACCTTGTC 
ATGTCAGTGG GCATTGGTGT TGAGTTCTGT GTACATTTAA CACATGCTTT CTCGGTGAGT 
AGTGGCGACA GAGACCAAAG AGTCAGGGAC GCTCTGGGTA CAATGGGAGC TTCTGTCTTC 
AGTGGCATCA CATTAACAAA ACTCGTTGGC GTGATTGTTC TTTGCTTCTC AAGAACAGAA 
GTTTTTGTGG TTTATTACTT CCAAATGTAC CTTGCACTGG TCCTCCTTGG TTTCTTACAT 
GGGTTGGTGT TCTTACCGGT TGTATTAAGC ATGTTTGGCC CCCCTTCAAG GTGTAAACTT 
GTCGAGAAGC AAGAAGACCG GCCATCGGTT TCCTTGCGGC CTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011034869.1
CDS	265..4149
Translation	MELSSRTMKLFLLSIFLLQVFYAVSIVSAERSDARSLKTRNAVSGEIHSQEYCAMYDICGAREDGKVVNCPFSSPSVKPDDLLSQKIQSLCPTITGNVCCSEAQFETLRSQVQQAIPFLVGCPACLRNFLNLFCELTCSPHQSMFINVTSTDKVKGNLTVSGIDFYVYDSFGEGLYESCKDVKFGTMNSRALNFIGAGAKNFTEWYAFIGRRAPLNVPGSPYAMTFKPSAPESSGMKPMNVSTYSCGDISLGCSCGDCPQSPVCANTDPPPHHEGASCAVRIGSLKAKCVDFILTILYVILVSIFLGWGLFHRKRERDQSSRMNPVSNIKDSGEVTGKKDENVTMQMLEDSPQTGSRVQLSIVQGYMSKFYRCYGTWVARNPILVLILSLAVILLLCLGLIRFKVETRPEKLWVGPGSKVAEEKRFFDTHLAPFYRIEQLILATVPEAGAQKRPSIVTENNIKLLFEIQKKVDGIHANHSGTMISLTDICLKPLDKDCATQSLLQYFQMDPQNLDNYGGVEHVNYCLQHYSSADTCRSAFKAPLDPSTALGGFSGNNYSEASAFIVTYPVNNVIDKEGNETDKVVAWEKAFIQLVKNELLPMVQSKNLTLSFSSESSIEEELKRESTADVITILISYLVMFAYISLTLGDTPHLSSFYISSKVLLGLSGVMLVMLSVLGSVGFFSAIGVKSTLIIMEVIPFLVLAVGVDNMCILVHAVKRQPMELPLEGRISNALVEVGPSITLASLSEVLAFAVGSFIPMPACRVFSMFAALAVLLDFLLQVTAFVAFIVFDFLRAEDKRIDCFPCRKISSSSADSDKGIGGRRPGLLARYMKEIHAPILSLWGVKIFVIAIFAAFTLSSIALSTRVQPGLEQKIVLPRDSYLQGYFNNVSEYLRIGPPLYFVVKNYNYSSESSQTNQLCSISHCNSNSLLNEIARASLTPESSYIAMPAASWLDDFLVWISPEAFGCCRKFTNGTYCPPDDQSPCCSSDTGSCGLGGICKDCTTCFRHSDLNNDRPSTSQFKEKLPWFLNALPSADCAKGGHGAYTSSIDLQGYENGVIEASSFRTYHTPLNKQIDYVNSMRAAREFSSRASDSLKMEIFPYSVFYMFFEQYLDIWRTALINLAIAIGAVFVVCLVITCSLWSSAIILLVLVMIVVDLMGVMAILNIQLNAVSVVNLVMSVGIGVEFCVHLTHAFSVSSGDRDQRVRDALGTMGASVFSGITLTKLVGVIVLCFSRTEVFVVYYFQMYLALVLLGFLHGLVFLPVVLSMFGPPSRCKLVEKQEDRPSVSLRP

Target ORF information:

RefSeq Version	XM_011036567.1
Organism	Populus euphratica(Euphrates poplar)
Definition	Populus euphratica Niemann-Pick C1 protein-like (LOC105132845), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011036567.1

ORF Insert Sequence:

ATGGAGCTCT CAAGCAGGAC GATGAAGCTT TTTCTGCTTT CGATTTTTCT ATTACAGGTG 
TTTTATGCAG TATCTATAGT CAGCGCGGAG AGGTCTGATG CACGGTCGCT TAAGACACGA 
AATGCAGTAT CAGGGGAGAT ACATTCACAG GAATATTGTG CAATGTATGA TATTTGTGGT 
GCACGTGAAG ATGGAAAAGT CGTGAACTGT CCTTTTAGCT CCCCATCTGT GAAGCCAGAT 
GACTTGCTTT CACAAAAGAT TCAAAGCCTA TGCCCTACAA TCACTGGCAA TGTTTGTTGT 
TCAGAAGCTC AGTTTGAAAC TTTACGGTCA CAAGTCCAGC AAGCAATTCC ATTTCTGGTC 
GGCTGTCCTG CATGTTTGAG AAACTTTTTG AATTTGTTCT GTGAGCTTAC CTGTTCCCCG 
CATCAGAGCA TGTTTATTAA CGTGACTTCT ACGGATAAGG TTAAAGGCAA TTTGACCGTG 
AGTGGCATTG ATTTTTATGT ATATGATTCT TTTGGTGAAG GGTTGTATGA ATCTTGCAAG 
GATGTAAAGT TTGGGACCAT GAATAGTCGG GCTCTAAATT TTATTGGTGC TGGTGCTAAA 
AATTTCACAG AGTGGTATGC ATTTATTGGT AGACGAGCAC CCCTTAATGT GCCGGGCTCA 
CCATATGCTA TGACATTCAA GCCTTCTGCT CCTGAGTCAT CTGGAATGAA ACCCATGAAT 
GTTTCTACAT ATTCATGTGG TGATATTTCA CTGGGTTGTT CTTGTGGTGA TTGTCCTCAA 
TCTCCTGTTT GTGCAAATAC TGATCCTCCT CCACATCACG AGGGGGCTTC TTGTGCGGTG 
AGAATTGGAT CTCTCAAGGC CAAATGTGTC GACTTTATTC TAACAATCCT CTATGTTATA 
TTGGTTTCCA TCTTTTTGGG ATGGGGCTTG TTTCATCGAA AAAGAGAAAG GGACCAGAGT 
TCTAGAATGA ATCCAGTGTC GAATATCAAG GACAGTGGTG AAGTTACTGG GAAGAAGGAT 
GAGAACGTTA CCATGCAGAT GCTTGAAGAT TCTCCTCAAA CCGGAAGCAG GGTTCAGCTT 
TCAATTGTTC AAGGATACAT GTCGAAGTTC TACAGGTGTT ATGGAACATG GGTTGCTAGA 
AACCCAATCC TTGTGTTGAT CTTGTCGTTG GCTGTAATTC TTTTACTTTG TCTAGGTCTC 
ATCCGTTTCA AGGTGGAGAC ACGGCCTGAG AAGCTATGGG TAGGGCCTGG GAGTAAAGTT 
GCGGAAGAGA AAAGGTTTTT TGACACCCAC CTAGCCCCCT TTTACAGAAT TGAGCAGCTA 
ATTCTAGCGA CTGTTCCAGA AGCTGGGGCA CAGAAAAGAC CAAGTATTGT GACAGAAAAC 
AACATCAAAT TACTTTTTGA AATACAAAAA AAGGTTGATG GAATCCATGC AAATCATTCT 
GGAACAATGA TATCTCTGAC TGATATTTGC TTGAAGCCAC TGGACAAAGA TTGTGCTACT 
CAGAGTTTAC TGCAGTATTT CCAAATGGAT CCTCAAAATC TCGATAATTA TGGAGGAGTT 
GAGCACGTGA ACTATTGTCT CCAGCATTAT TCCTCTGCAG ACACATGTCG GAGTGCCTTC 
AAAGCTCCCC TTGATCCAAG CACAGCCTTG GGAGGTTTCT CTGGGAACAA TTATTCGGAG 
GCATCTGCAT TTATTGTAAC TTATCCAGTA AATAATGTGA TTGATAAAGA AGGGAATGAA 
ACTGATAAGG TGGTTGCTTG GGAGAAGGCT TTTATTCAGT TAGTGAAGAA TGAATTATTG 
CCAATGGTGC AATCTAAGAA TCTCACTCTT TCTTTTTCTT CGGAAAGCTC CATTGAGGAG 
GAACTAAAAA GAGAAAGCAC TGCTGATGTC ATAACTATAT TGATTAGCTA TCTTGTGATG 
TTTGCCTACA TCTCTCTTAC TTTGGGTGAC ACTCCTCATT TATCTTCGTT TTACATTTCA 
TCTAAGGTAT TGCTTGGTCT TTCTGGAGTT ATGCTTGTCA TGTTGTCTGT TCTTGGATCT 
GTTGGATTTT TTAGTGCCAT TGGAGTGAAG TCTACACTAA TCATCATGGA AGTCATTCCT 
TTTCTTGTAT TAGCTGTTGG GGTGGATAAC ATGTGCATAT TGGTGCATGC TGTCAAGAGG 
CAACCAATGG AGTTGCCGCT AGAGGGACGG ATAAGCAATG CTCTTGTGGA AGTTGGACCA 
TCCATCACAC TTGCTAGTCT ATCCGAGGTT TTAGCATTTG CAGTTGGAAG TTTCATTCCT 
ATGCCAGCAT GCCGTGTGTT CTCTATGTTC GCTGCACTTG CTGTTCTTTT GGACTTTCTT 
TTGCAAGTTA CTGCCTTTGT AGCTTTCATA GTTTTTGATT TTTTGCGAGC TGAGGATAAG 
AGAATTGATT GCTTTCCATG TCGGAAAATC TCATCTTCAT CTGCAGACTC TGATAAAGGT 
ATTGGTGGGA GAAGACCTGG ATTGCTGGCT CGCTATATGA AGGAGATCCA CGCACCCATA 
CTCAGTCTTT GGGGAGTTAA GATATTTGTC ATTGCCATCT TTGCTGCTTT CACATTGTCC 
AGCATAGCAT TATCCACTCG AGTTCAACCT GGTTTAGAAC AAAAAATTGT TCTTCCGCGA 
GACTCATATC TTCAGGGCTA TTTTAATAAT GTCTCAGAGT ATCTCAGAAT TGGTCCGCCA 
CTATATTTTG TAGTGAAGAA CTACAACTAT AGCTCAGAAT CAAGTCAGAC AAATCAGCTG 
TGCTCCATCA GCCACTGCAA TTCAAACTCT CTTTTAAATG AGATTGCAAG AGCATCCTTA 
ACACCAGAAT CTAGCTATAT AGCTATGCCA GCTGCTTCAT GGCTTGATGA TTTTCTTGTA 
TGGATATCTC CTGAAGCTTT TGGCTGCTGT AGGAAGTTCA CAAATGGGAC TTATTGCCCC 
CCTGATGATC AGTCTCCTTG TTGTTCATCT GATACAGGTT CATGTGGTCT TGGTGGGATA 
TGCAAAGATT GTACTACTTG CTTCCGTCAC TCAGATTTGA ATAATGATCG TCCATCTACA 
TCTCAGTTCA AGGAGAAACT CCCATGGTTC CTCAATGCTT TGCCCTCTGC TGACTGTGCC 
AAAGGCGGCC ATGGTGCTTA CACTAGCAGT ATAGATCTAC AAGGCTATGA GAATGGTGTT 
ATTGAAGCAT CATCCTTCCG TACATATCAT ACACCTCTTA ACAAGCAGAT TGACTATGTC 
AATTCAATGA GGGCTGCTCG AGAGTTCAGT TCAAGGGCTT CTGATTCTTT GAAGATGGAG 
ATCTTCCCAT ACTCTGTATT TTATATGTTT TTTGAACAAT ACCTTGATAT ATGGAGGACT 
GCATTGATCA ACCTCGCCAT AGCAATTGGA GCGGTATTTG TTGTTTGCTT GGTCATCACT 
TGCAGTTTGT GGAGTTCTGC TATCATTTTG CTGGTGTTGG TAATGATAGT CGTGGATCTC 
ATGGGCGTGA TGGCAATTCT GAACATTCAA CTGAATGCAG TCTCTGTTGT CAACCTTGTC 
ATGTCAGTGG GCATTGGTGT TGAGTTCTGT GTACATTTAA CACATGCTTT CTCGGTGAGT 
AGTGGCGACA GAGACCAAAG AGTCAGGGAC GCTCTGGGTA CAATGGGAGC TTCTGTCTTC 
AGTGGCATCA CATTAACAAA ACTCGTTGGC GTGATTGTTC TTTGCTTCTC AAGAACAGAA 
GTTTTTGTGG TTTATTACTT CCAAATGTAC CTTGCACTGG TCCTCCTTGG TTTCTTACAT 
GGGTTGGTGT TCTTACCGGT TGTATTAAGC ATGTTTGGCC CCCCTTCAAG GTGTAAACTT 
GTCGAGAAGC AAGAAGACCG GCCATCGGTT TCCTTGCGGC CTTGA