impg2 cDNA ORF clone, Poecilia reticulata(guppy)

Gene
Clones
gRNAs

Summary

Gene Symbol	impg2
Entrez Gene ID	103479498
Full Name	interphotoreceptor matrix proteoglycan 2
Gene Type	protein-coding
Organism	Poecilia reticulata(guppy)

mRNA and Protein(s)

mRNA	Protein	Name
XM_008433969.2	XP_008432191.1	interphotoreceptor matrix proteoglycan 2 isoform X1
XM_008433979.2	XP_008432201.1	interphotoreceptor matrix proteoglycan 2 isoform X2

Pathways from BioSystems
Gene Ontology

The following impg2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the impg2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OPo185873	XM_008433969.2 Latest version!	Poecilia reticulata interphotoreceptor matrix proteoglycan 2 (impg2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OPo185874	XM_008433979.2 Latest version!	Poecilia reticulata interphotoreceptor matrix proteoglycan 2 (impg2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

impg2 ( XM_008433969.2 ) cDNA ORF clone, Poecilia reticulata(guppy) -> XP_008432191.1 Poecilia reticulata interphotoreceptor matrix proteoglycan 2 (impg2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OPo185873
Clone ID Related Accession (Same CDS sequence)	XM_008433969.2
Accession Version	XM_008433969.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3369bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-20
Organism	Poecilia reticulata(guppy)
Product	interphotoreceptor matrix proteoglycan 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_024332.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 21, 2016 this sequence version replaced XM_008433969.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Poecilia reticulata Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTAGCG CTTCCTGGAG AAGTGTTTTC TTTTGTGCTC TCTTTAGCCT CACAGTCATA 
GTTTTGAGCA GACTCTCAGA TGCCACACTG GAAGGCTCCT CTATGGGATA CCTGGGTAAG 
CCTTTTTCTC CAGAGCCGGC GGCGTTTCCT CAAATCATCC GGGTGTCCGA TGTGAAGGCA 
TCTCACGACG GCCATGCCGT CACCTCTCGA AGGAAAAGGA ATATCTTTTA TCAGAGCGGA 
CTCAGACTAT GTGGACAAGA GACTGCTGAG GAGGTTGTTG CAAACCATTT GGGCTATTTT 
CACCTCAGAG TGTGCCAGGA AACCGTGTGG GAGGCTTTCA AGATCTTCTG GGATCGACTG 
CCTGAGCAAG ACGAGTACCA AAGCTGGATG AGTCAGTGCC AGGAAGGCAC AGTCACTGTG 
CAGGATATTG GCAGCTACTT CAGCCAGTCA GAGGAGCATC AGGCTCTGGT CAAAGAGAGA 
ATGTCGCATT CAGTTTTTAA AAGTGAGCCC ACCCGGTCCT GGAAGCATAT GTGCAGCACA 
CCAACACCAG CGAGTTCAGA AACAGTCGAA GCCCCAGAGT TAGAGGAAAT AGATAAAGGA 
AAGGACATCG CAGACGTCAT TTCACCAGAG GTGGAAGAGA ATCCACTGTT CAATGAAATC 
ACCATTCAGC CCACGGCTGC CCCCGTTCTG GAGCAGGTGG TGGAGCTGAG CATCCTCCTG 
ACCGGAGAGG TCTTCAGCGA CGAGCTCAGA GATCCAGCCA GCCTCAAGTT CCAGACGCTC 
AGCAGAGATC TTGCGGAAAA GATAGAAGAT GCTCTAGAGG GGCTTCCCGG CTTTAAGAGT 
GTGTCTGTAA TCGACTTCAG ACCACAGAAG GACTCTCAGT GGGTGGATGG CATAGTGGTG 
GATTACGCAG TCACAGTAGC GGTGGACGGA GCCGGGGTGA GCTCTGAACA GCTGAACTAC 
CTGACCCTGC AGTCCAACCT GGTGGAGAAC TCGTATCGAG AGGTCAAGGA GCCCCCCACA 
GTGGTCTACA CAATCAGCGA GATTAAAAAC ATCTTCACCG AACCGCATCA CATCAATGAG 
CTGGAGAGTG TGTCTGAGAT TCCTGAGCCT GTCAGCGCTG CAGACGAAGC GACGGGCATT 
GACCTATCAG AAGTCTTGAC CCTGGATCAT CCCACTGGCG GTGGATCATC ACCACCAGTA 
GAACTCAACA CCCTGGGACC AGAAGAGGAC ATCGTTATTC TGGAGGAGAG TGAACTAGCG 
TCACCTGAGG CAGCACTCGC CACCAGTCCA CCTGAAGCTG GTAGCATTGA GGAGGAAGAT 
CTGTTGCCAG AGGATGTTGT GCTAGCTCCT GCTCCAAAGA CCATCCCGCC TATAGAGCTC 
CTACCAGAGC CTCCCTCCCA RGAGACGCCA AAGAGCTCCA CGTTGCCTGA GCCGCCAATA 
GAAACAGAGG CTTCTGGGTC AGGCCGAGAA AACCTGGACA GTTTAATTGA GCCATCCATT 
GAAGAAGAAG AAGACCAAAT ATCTCTTGTA GAGACTGAAG ATGTCAAATC AGAACCTGAA 
CAAGAAACAG TTGATGAGGT TGAGATTTTG GAAAAACCTG AAGTCTCTAC AGTTTTAACT 
AAGACCACAA TAGAACAAGG CCAGCCAGCT CTTTTACCCC CAACAGAGAC TGTGAATATC 
CCAACTGAGG TGTCGGAGCC TGATGTAGCC AAAACCACGC TGCACTCTGA TGTTGATGAA 
CCTGTGGTTC AGGCACCAGA GGACTCTAGC AAGTTTGACA AAGACCCTGA CCAGACTGTA 
GGGATCAATG TGATTTTTAC CTATCCTGAG GAACCTGCAT TTGATGAAAA AGGCTTTGAC 
GAGACAATTG AGAGTGATGG AAGTGAAGTG AAAGAGGGAT CTTTGTCTGA AACCAAGGAT 
ATAGAGGAGG AGGCACCTCT GACCCCAGAG ATCTTAGCAG GAGATCTTGT GGAGGATGAG 
ATTTTGCTCG TGAACAGAGA CAATTCGAAG TTACCCGGGA CAGACTTCCC ACGCCATCCA 
CTGCCAACTG CCCTGTCTCC AGAGGAGGAA TCCCCTTTTA CAGTCTTCTC ATCCATTGTC 
CCAGATTTTG ATGCCCTACC TGACAAAGCC ATCACTTCAC TGACCACTCA GGAGGACCTG 
GATGATGCTG CCCCTGAAGA TCACGGATAT GACCTGATTC CTTATGATTA CGGCTTGACA 
AACCAGACAG AGGAAGGCAG CACTGGATCC CCATTCAGTG TGGCCCAAGG CACAGACCAA 
GTCAGCATTG CCATGCCTAT AAACCCGAGG CGAGCTCTGA TAGTCTTCTT CAGCCTGAGA 
GTGACCAACA TGGTATTTTC TGAAGATCTC TTTAACAAGA GCTCCCCAGA GTACAAGGCT 
TTGGAGCAGC GCTTCCTAGA ACTGCTTGTG CCCTATCTTC AGTCCAACCT GAGCCACTTT 
GAGAATCTAG AGATCTTGAA CTTCAGGAAT GGGAGCATCG TGGTCAACAG TCGGATGAAG 
TTTGGGAGGC CTGTGGCCCG GGGCGTCACC ACCACCGTGT ATCTAATCCT GGAGGACTTC 
TGTAACACTG CGTACCAGAC CATGAACCTA GCCATTGATA AATACTCTCT GGATGTGGAG 
TCAGGTGACC AAGCCGACCC CTGCAAGTTC CAGGCGTGCA ACGAGTACGC AGAATGTAAA 
GTGAACAAGT GGTCAGGGGA AGCGGAGTGC GTCTGTAACG CGGGCTACTT CAGTGTCGAC 
GGCCTTCCGT GTCAAAGCAT CTGTGAGCTG CAGACAGACT TTTGCCTCAA TGACGGCAAG 
TGTGACATCA TCCCTGGCCA GGGAGCCATC TGCAGGTGTC GTGTTGGAGA GAACTGGTGG 
TACAGAGGTG AACACTGTGA GGAGTATGTG TCCGAGCCTC TGGTGGTTGG CATCGCCATA 
GCCTCTGTTG CTGGTTTCCT GTTTGTGGCG TCCGGAGTGC TGTTCTTCCT GGCCAGGACA 
CTTCGGGATC AGTACGACAA GGAAGAGTCG GAGGATCCCG TACGGCGGGT GGAAAGTCTT 
CCGTCATTGG AAAGGGCAAC CAAATACAAC CCCATGTATG AAAGTGAAGC CACTACAGGC 
TACAGCCACT ACTACCGTCG TTATCCCGAA GCGCCTATGT ACAGCAGTGC CAGCGCCGAG 
GCATCCACCG ATTTTAGCAG CGAGGAGATA CGGCACATCT ATGAAAACAG CGAGCTGACC 
AAAGAGGAAA TCCAAGACAG GATACGCATC ATTGAACTCT ACGCTAAGGA CCGGCAGTTT 
GCAGACTTTG TGCGGCAGCA TCAAGCAGTT CTGGATACCC AGAGGGAAAG CTCCTCTATG 
CACTCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008432191.1
CDS	402..3770
Translation	MSSASWRSVFFCALFSLTVIVLSRLSDATLEGSSMGYLGKPFSPEPAAFPQIIRVSDVKASHDGHAVTSRRKRNIFYQSGLRLCGQETAEEVVANHLGYFHLRVCQETVWEAFKIFWDRLPEQDEYQSWMSQCQEGTVTVQDIGSYFSQSEEHQALVKERMSHSVFKSEPTRSWKHMCSTPTPASSETVEAPELEEIDKGKDIADVISPEVEENPLFNEITIQPTAAPVLEQVVELSILLTGEVFSDELRDPASLKFQTLSRDLAEKIEDALEGLPGFKSVSVIDFRPQKDSQWVDGIVVDYAVTVAVDGAGVSSEQLNYLTLQSNLVENSYREVKEPPTVVYTISEIKNIFTEPHHINELESVSEIPEPVSAADEATGIDLSEVLTLDHPTGGGSSPPVELNTLGPEEDIVILEESELASPEAALATSPPEAGSIEEEDLLPEDVVLAPAPKTIPPIELLPEPPSnullETPKSSTLPEPPIETEASGSGRENLDSLIEPSIEEEEDQISLVETEDVKSEPEQETVDEVEILEKPEVSTVLTKTTIEQGQPALLPPTETVNIPTEVSEPDVAKTTLHSDVDEPVVQAPEDSSKFDKDPDQTVGINVIFTYPEEPAFDEKGFDETIESDGSEVKEGSLSETKDIEEEAPLTPEILAGDLVEDEILLVNRDNSKLPGTDFPRHPLPTALSPEEESPFTVFSSIVPDFDALPDKAITSLTTQEDLDDAAPEDHGYDLIPYDYGLTNQTEEGSTGSPFSVAQGTDQVSIAMPINPRRALIVFFSLRVTNMVFSEDLFNKSSPEYKALEQRFLELLVPYLQSNLSHFENLEILNFRNGSIVVNSRMKFGRPVARGVTTTVYLILEDFCNTAYQTMNLAIDKYSLDVESGDQADPCKFQACNEYAECKVNKWSGEAECVCNAGYFSVDGLPCQSICELQTDFCLNDGKCDIIPGQGAICRCRVGENWWYRGEHCEEYVSEPLVVGIAIASVAGFLFVASGVLFFLARTLRDQYDKEESEDPVRRVESLPSLERATKYNPMYESEATTGYSHYYRRYPEAPMYSSASAEASTDFSSEEIRHIYENSELTKEEIQDRIRIIELYAKDRQFADFVRQHQAVLDTQRESSSMHS

Target ORF information:

RefSeq Version	XM_008433969.2
Organism	Poecilia reticulata(guppy)
Definition	Poecilia reticulata interphotoreceptor matrix proteoglycan 2 (impg2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008433969.2

ORF Insert Sequence:

ATGAGTAGCG CTTCCTGGAG AAGTGTTTTC TTTTGTGCTC TCTTTAGCCT CACAGTCATA 
GTTTTGAGCA GACTCTCAGA TGCCACACTG GAAGGCTCCT CTATGGGATA CCTGGGTAAG 
CCTTTTTCTC CAGAGCCGGC GGCGTTTCCT CAAATCATCC GGGTGTCCGA TGTGAAGGCA 
TCTCACGACG GCCATGCCGT CACCTCTCGA AGGAAAAGGA ATATCTTTTA TCAGAGCGGA 
CTCAGACTAT GTGGACAAGA GACTGCTGAG GAGGTTGTTG CAAACCATTT GGGCTATTTT 
CACCTCAGAG TGTGCCAGGA AACCGTGTGG GAGGCTTTCA AGATCTTCTG GGATCGACTG 
CCTGAGCAAG ACGAGTACCA AAGCTGGATG AGTCAGTGCC AGGAAGGCAC AGTCACTGTG 
CAGGATATTG GCAGCTACTT CAGCCAGTCA GAGGAGCATC AGGCTCTGGT CAAAGAGAGA 
ATGTCGCATT CAGTTTTTAA AAGTGAGCCC ACCCGGTCCT GGAAGCATAT GTGCAGCACA 
CCAACACCAG CGAGTTCAGA AACAGTCGAA GCCCCAGAGT TAGAGGAAAT AGATAAAGGA 
AAGGACATCG CAGACGTCAT TTCACCAGAG GTGGAAGAGA ATCCACTGTT CAATGAAATC 
ACCATTCAGC CCACGGCTGC CCCCGTTCTG GAGCAGGTGG TGGAGCTGAG CATCCTCCTG 
ACCGGAGAGG TCTTCAGCGA CGAGCTCAGA GATCCAGCCA GCCTCAAGTT CCAGACGCTC 
AGCAGAGATC TTGCGGAAAA GATAGAAGAT GCTCTAGAGG GGCTTCCCGG CTTTAAGAGT 
GTGTCTGTAA TCGACTTCAG ACCACAGAAG GACTCTCAGT GGGTGGATGG CATAGTGGTG 
GATTACGCAG TCACAGTAGC GGTGGACGGA GCCGGGGTGA GCTCTGAACA GCTGAACTAC 
CTGACCCTGC AGTCCAACCT GGTGGAGAAC TCGTATCGAG AGGTCAAGGA GCCCCCCACA 
GTGGTCTACA CAATCAGCGA GATTAAAAAC ATCTTCACCG AACCGCATCA CATCAATGAG 
CTGGAGAGTG TGTCTGAGAT TCCTGAGCCT GTCAGCGCTG CAGACGAAGC GACGGGCATT 
GACCTATCAG AAGTCTTGAC CCTGGATCAT CCCACTGGCG GTGGATCATC ACCACCAGTA 
GAACTCAACA CCCTGGGACC AGAAGAGGAC ATCGTTATTC TGGAGGAGAG TGAACTAGCG 
TCACCTGAGG CAGCACTCGC CACCAGTCCA CCTGAAGCTG GTAGCATTGA GGAGGAAGAT 
CTGTTGCCAG AGGATGTTGT GCTAGCTCCT GCTCCAAAGA CCATCCCGCC TATAGAGCTC 
CTACCAGAGC CTCCCTCCCA RGAGACGCCA AAGAGCTCCA CGTTGCCTGA GCCGCCAATA 
GAAACAGAGG CTTCTGGGTC AGGCCGAGAA AACCTGGACA GTTTAATTGA GCCATCCATT 
GAAGAAGAAG AAGACCAAAT ATCTCTTGTA GAGACTGAAG ATGTCAAATC AGAACCTGAA 
CAAGAAACAG TTGATGAGGT TGAGATTTTG GAAAAACCTG AAGTCTCTAC AGTTTTAACT 
AAGACCACAA TAGAACAAGG CCAGCCAGCT CTTTTACCCC CAACAGAGAC TGTGAATATC 
CCAACTGAGG TGTCGGAGCC TGATGTAGCC AAAACCACGC TGCACTCTGA TGTTGATGAA 
CCTGTGGTTC AGGCACCAGA GGACTCTAGC AAGTTTGACA AAGACCCTGA CCAGACTGTA 
GGGATCAATG TGATTTTTAC CTATCCTGAG GAACCTGCAT TTGATGAAAA AGGCTTTGAC 
GAGACAATTG AGAGTGATGG AAGTGAAGTG AAAGAGGGAT CTTTGTCTGA AACCAAGGAT 
ATAGAGGAGG AGGCACCTCT GACCCCAGAG ATCTTAGCAG GAGATCTTGT GGAGGATGAG 
ATTTTGCTCG TGAACAGAGA CAATTCGAAG TTACCCGGGA CAGACTTCCC ACGCCATCCA 
CTGCCAACTG CCCTGTCTCC AGAGGAGGAA TCCCCTTTTA CAGTCTTCTC ATCCATTGTC 
CCAGATTTTG ATGCCCTACC TGACAAAGCC ATCACTTCAC TGACCACTCA GGAGGACCTG 
GATGATGCTG CCCCTGAAGA TCACGGATAT GACCTGATTC CTTATGATTA CGGCTTGACA 
AACCAGACAG AGGAAGGCAG CACTGGATCC CCATTCAGTG TGGCCCAAGG CACAGACCAA 
GTCAGCATTG CCATGCCTAT AAACCCGAGG CGAGCTCTGA TAGTCTTCTT CAGCCTGAGA 
GTGACCAACA TGGTATTTTC TGAAGATCTC TTTAACAAGA GCTCCCCAGA GTACAAGGCT 
TTGGAGCAGC GCTTCCTAGA ACTGCTTGTG CCCTATCTTC AGTCCAACCT GAGCCACTTT 
GAGAATCTAG AGATCTTGAA CTTCAGGAAT GGGAGCATCG TGGTCAACAG TCGGATGAAG 
TTTGGGAGGC CTGTGGCCCG GGGCGTCACC ACCACCGTGT ATCTAATCCT GGAGGACTTC 
TGTAACACTG CGTACCAGAC CATGAACCTA GCCATTGATA AATACTCTCT GGATGTGGAG 
TCAGGTGACC AAGCCGACCC CTGCAAGTTC CAGGCGTGCA ACGAGTACGC AGAATGTAAA 
GTGAACAAGT GGTCAGGGGA AGCGGAGTGC GTCTGTAACG CGGGCTACTT CAGTGTCGAC 
GGCCTTCCGT GTCAAAGCAT CTGTGAGCTG CAGACAGACT TTTGCCTCAA TGACGGCAAG 
TGTGACATCA TCCCTGGCCA GGGAGCCATC TGCAGGTGTC GTGTTGGAGA GAACTGGTGG 
TACAGAGGTG AACACTGTGA GGAGTATGTG TCCGAGCCTC TGGTGGTTGG CATCGCCATA 
GCCTCTGTTG CTGGTTTCCT GTTTGTGGCG TCCGGAGTGC TGTTCTTCCT GGCCAGGACA 
CTTCGGGATC AGTACGACAA GGAAGAGTCG GAGGATCCCG TACGGCGGGT GGAAAGTCTT 
CCGTCATTGG AAAGGGCAAC CAAATACAAC CCCATGTATG AAAGTGAAGC CACTACAGGC 
TACAGCCACT ACTACCGTCG TTATCCCGAA GCGCCTATGT ACAGCAGTGC CAGCGCCGAG 
GCATCCACCG ATTTTAGCAG CGAGGAGATA CGGCACATCT ATGAAAACAG CGAGCTGACC 
AAAGAGGAAA TCCAAGACAG GATACGCATC ATTGAACTCT ACGCTAAGGA CCGGCAGTTT 
GCAGACTTTG TGCGGCAGCA TCAAGCAGTT CTGGATACCC AGAGGGAAAG CTCCTCTATG 
CACTCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

impg2 ( XM_008433979.2 ) cDNA ORF clone, Poecilia reticulata(guppy) -> XP_008432201.1 Poecilia reticulata interphotoreceptor matrix proteoglycan 2 (impg2), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OPo185874
Clone ID Related Accession (Same CDS sequence)	XM_008433979.2
Accession Version	XM_008433979.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3336bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-20
Organism	Poecilia reticulata(guppy)
Product	interphotoreceptor matrix proteoglycan 2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_024332.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 21, 2016 this sequence version replaced XM_008433979.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Poecilia reticulata Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTAGCG CTTCCTGGAG AAGTGTTTTC TTTTGTGCTC TCTTTAGCCT CACAGTCATA 
GTTTTGAGCA GACTCTCAGA TGCCACACTG GAAGGCTCCT CTATGGGATA CCTGGGTAAG 
CCTTTTTCTC CAGAGCCGGC GGCGTTTCCT CAAATCATCC GGGTGTCCGA TGTGAAGGCA 
TCTCACGACG GCCATGCCGT CACCTCTCGA AGGAAAAGGA ATATCTTTTA TCAGAGCGGA 
CTCAGACTAT GTGGACAAGA GACTGCTGAG GAGGTTGTTG CAAACCATTT GGGCTATTTT 
CACCTCAGAG TGTGCCAGGA AACCGTGTGG GAGGCTTTCA AGATCTTCTG GGATCGACTG 
CCTGAGCAAG ACGAGTACCA AAGCTGGATG AGTCAGTGCC AGGAAGGCAC AGTCACTGTG 
CAGGATATTG GCAGCTACTT CAGCCAGTCA GAGGAGCATC AGGCTCTGGT CAAAGAGAGA 
ATGTCGCATT CAGTTTTTAA AAGCACACCA ACACCAGCGA GTTCAGAAAC AGTCGAAGCC 
CCAGAGTTAG AGGAAATAGA TAAAGGAAAG GACATCGCAG ACGTCATTTC ACCAGAGGTG 
GAAGAGAATC CACTGTTCAA TGAAATCACC ATTCAGCCCA CGGCTGCCCC CGTTCTGGAG 
CAGGTGGTGG AGCTGAGCAT CCTCCTGACC GGAGAGGTCT TCAGCGACGA GCTCAGAGAT 
CCAGCCAGCC TCAAGTTCCA GACGCTCAGC AGAGATCTTG CGGAAAAGAT AGAAGATGCT 
CTAGAGGGGC TTCCCGGCTT TAAGAGTGTG TCTGTAATCG ACTTCAGACC ACAGAAGGAC 
TCTCAGTGGG TGGATGGCAT AGTGGTGGAT TACGCAGTCA CAGTAGCGGT GGACGGAGCC 
GGGGTGAGCT CTGAACAGCT GAACTACCTG ACCCTGCAGT CCAACCTGGT GGAGAACTCG 
TATCGAGAGG TCAAGGAGCC CCCCACAGTG GTCTACACAA TCAGCGAGAT TAAAAACATC 
TTCACCGAAC CGCATCACAT CAATGAGCTG GAGAGTGTGT CTGAGATTCC TGAGCCTGTC 
AGCGCTGCAG ACGAAGCGAC GGGCATTGAC CTATCAGAAG TCTTGACCCT GGATCATCCC 
ACTGGCGGTG GATCATCACC ACCAGTAGAA CTCAACACCC TGGGACCAGA AGAGGACATC 
GTTATTCTGG AGGAGAGTGA ACTAGCGTCA CCTGAGGCAG CACTCGCCAC CAGTCCACCT 
GAAGCTGGTA GCATTGAGGA GGAAGATCTG TTGCCAGAGG ATGTTGTGCT AGCTCCTGCT 
CCAAAGACCA TCCCGCCTAT AGAGCTCCTA CCAGAGCCTC CCTCCCARGA GACGCCAAAG 
AGCTCCACGT TGCCTGAGCC GCCAATAGAA ACAGAGGCTT CTGGGTCAGG CCGAGAAAAC 
CTGGACAGTT TAATTGAGCC ATCCATTGAA GAAGAAGAAG ACCAAATATC TCTTGTAGAG 
ACTGAAGATG TCAAATCAGA ACCTGAACAA GAAACAGTTG ATGAGGTTGA GATTTTGGAA 
AAACCTGAAG TCTCTACAGT TTTAACTAAG ACCACAATAG AACAAGGCCA GCCAGCTCTT 
TTACCCCCAA CAGAGACTGT GAATATCCCA ACTGAGGTGT CGGAGCCTGA TGTAGCCAAA 
ACCACGCTGC ACTCTGATGT TGATGAACCT GTGGTTCAGG CACCAGAGGA CTCTAGCAAG 
TTTGACAAAG ACCCTGACCA GACTGTAGGG ATCAATGTGA TTTTTACCTA TCCTGAGGAA 
CCTGCATTTG ATGAAAAAGG CTTTGACGAG ACAATTGAGA GTGATGGAAG TGAAGTGAAA 
GAGGGATCTT TGTCTGAAAC CAAGGATATA GAGGAGGAGG CACCTCTGAC CCCAGAGATC 
TTAGCAGGAG ATCTTGTGGA GGATGAGATT TTGCTCGTGA ACAGAGACAA TTCGAAGTTA 
CCCGGGACAG ACTTCCCACG CCATCCACTG CCAACTGCCC TGTCTCCAGA GGAGGAATCC 
CCTTTTACAG TCTTCTCATC CATTGTCCCA GATTTTGATG CCCTACCTGA CAAAGCCATC 
ACTTCACTGA CCACTCAGGA GGACCTGGAT GATGCTGCCC CTGAAGATCA CGGATATGAC 
CTGATTCCTT ATGATTACGG CTTGACAAAC CAGACAGAGG AAGGCAGCAC TGGATCCCCA 
TTCAGTGTGG CCCAAGGCAC AGACCAAGTC AGCATTGCCA TGCCTATAAA CCCGAGGCGA 
GCTCTGATAG TCTTCTTCAG CCTGAGAGTG ACCAACATGG TATTTTCTGA AGATCTCTTT 
AACAAGAGCT CCCCAGAGTA CAAGGCTTTG GAGCAGCGCT TCCTAGAACT GCTTGTGCCC 
TATCTTCAGT CCAACCTGAG CCACTTTGAG AATCTAGAGA TCTTGAACTT CAGGAATGGG 
AGCATCGTGG TCAACAGTCG GATGAAGTTT GGGAGGCCTG TGGCCCGGGG CGTCACCACC 
ACCGTGTATC TAATCCTGGA GGACTTCTGT AACACTGCGT ACCAGACCAT GAACCTAGCC 
ATTGATAAAT ACTCTCTGGA TGTGGAGTCA GGTGACCAAG CCGACCCCTG CAAGTTCCAG 
GCGTGCAACG AGTACGCAGA ATGTAAAGTG AACAAGTGGT CAGGGGAAGC GGAGTGCGTC 
TGTAACGCGG GCTACTTCAG TGTCGACGGC CTTCCGTGTC AAAGCATCTG TGAGCTGCAG 
ACAGACTTTT GCCTCAATGA CGGCAAGTGT GACATCATCC CTGGCCAGGG AGCCATCTGC 
AGGTGTCGTG TTGGAGAGAA CTGGTGGTAC AGAGGTGAAC ACTGTGAGGA GTATGTGTCC 
GAGCCTCTGG TGGTTGGCAT CGCCATAGCC TCTGTTGCTG GTTTCCTGTT TGTGGCGTCC 
GGAGTGCTGT TCTTCCTGGC CAGGACACTT CGGGATCAGT ACGACAAGGA AGAGTCGGAG 
GATCCCGTAC GGCGGGTGGA AAGTCTTCCG TCATTGGAAA GGGCAACCAA ATACAACCCC 
ATGTATGAAA GTGAAGCCAC TACAGGCTAC AGCCACTACT ACCGTCGTTA TCCCGAAGCG 
CCTATGTACA GCAGTGCCAG CGCCGAGGCA TCCACCGATT TTAGCAGCGA GGAGATACGG 
CACATCTATG AAAACAGCGA GCTGACCAAA GAGGAAATCC AAGACAGGAT ACGCATCATT 
GAACTCTACG CTAAGGACCG GCAGTTTGCA GACTTTGTGC GGCAGCATCA AGCAGTTCTG 
GATACCCAGA GGGAAAGCTC CTCTATGCAC TCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008432201.1
CDS	402..3737
Translation	MSSASWRSVFFCALFSLTVIVLSRLSDATLEGSSMGYLGKPFSPEPAAFPQIIRVSDVKASHDGHAVTSRRKRNIFYQSGLRLCGQETAEEVVANHLGYFHLRVCQETVWEAFKIFWDRLPEQDEYQSWMSQCQEGTVTVQDIGSYFSQSEEHQALVKERMSHSVFKSTPTPASSETVEAPELEEIDKGKDIADVISPEVEENPLFNEITIQPTAAPVLEQVVELSILLTGEVFSDELRDPASLKFQTLSRDLAEKIEDALEGLPGFKSVSVIDFRPQKDSQWVDGIVVDYAVTVAVDGAGVSSEQLNYLTLQSNLVENSYREVKEPPTVVYTISEIKNIFTEPHHINELESVSEIPEPVSAADEATGIDLSEVLTLDHPTGGGSSPPVELNTLGPEEDIVILEESELASPEAALATSPPEAGSIEEEDLLPEDVVLAPAPKTIPPIELLPEPPSnullETPKSSTLPEPPIETEASGSGRENLDSLIEPSIEEEEDQISLVETEDVKSEPEQETVDEVEILEKPEVSTVLTKTTIEQGQPALLPPTETVNIPTEVSEPDVAKTTLHSDVDEPVVQAPEDSSKFDKDPDQTVGINVIFTYPEEPAFDEKGFDETIESDGSEVKEGSLSETKDIEEEAPLTPEILAGDLVEDEILLVNRDNSKLPGTDFPRHPLPTALSPEEESPFTVFSSIVPDFDALPDKAITSLTTQEDLDDAAPEDHGYDLIPYDYGLTNQTEEGSTGSPFSVAQGTDQVSIAMPINPRRALIVFFSLRVTNMVFSEDLFNKSSPEYKALEQRFLELLVPYLQSNLSHFENLEILNFRNGSIVVNSRMKFGRPVARGVTTTVYLILEDFCNTAYQTMNLAIDKYSLDVESGDQADPCKFQACNEYAECKVNKWSGEAECVCNAGYFSVDGLPCQSICELQTDFCLNDGKCDIIPGQGAICRCRVGENWWYRGEHCEEYVSEPLVVGIAIASVAGFLFVASGVLFFLARTLRDQYDKEESEDPVRRVESLPSLERATKYNPMYESEATTGYSHYYRRYPEAPMYSSASAEASTDFSSEEIRHIYENSELTKEEIQDRIRIIELYAKDRQFADFVRQHQAVLDTQRESSSMHS

Target ORF information:

RefSeq Version	XM_008433979.2
Organism	Poecilia reticulata(guppy)
Definition	Poecilia reticulata interphotoreceptor matrix proteoglycan 2 (impg2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008433979.2

ORF Insert Sequence:

ATGAGTAGCG CTTCCTGGAG AAGTGTTTTC TTTTGTGCTC TCTTTAGCCT CACAGTCATA 
GTTTTGAGCA GACTCTCAGA TGCCACACTG GAAGGCTCCT CTATGGGATA CCTGGGTAAG 
CCTTTTTCTC CAGAGCCGGC GGCGTTTCCT CAAATCATCC GGGTGTCCGA TGTGAAGGCA 
TCTCACGACG GCCATGCCGT CACCTCTCGA AGGAAAAGGA ATATCTTTTA TCAGAGCGGA 
CTCAGACTAT GTGGACAAGA GACTGCTGAG GAGGTTGTTG CAAACCATTT GGGCTATTTT 
CACCTCAGAG TGTGCCAGGA AACCGTGTGG GAGGCTTTCA AGATCTTCTG GGATCGACTG 
CCTGAGCAAG ACGAGTACCA AAGCTGGATG AGTCAGTGCC AGGAAGGCAC AGTCACTGTG 
CAGGATATTG GCAGCTACTT CAGCCAGTCA GAGGAGCATC AGGCTCTGGT CAAAGAGAGA 
ATGTCGCATT CAGTTTTTAA AAGCACACCA ACACCAGCGA GTTCAGAAAC AGTCGAAGCC 
CCAGAGTTAG AGGAAATAGA TAAAGGAAAG GACATCGCAG ACGTCATTTC ACCAGAGGTG 
GAAGAGAATC CACTGTTCAA TGAAATCACC ATTCAGCCCA CGGCTGCCCC CGTTCTGGAG 
CAGGTGGTGG AGCTGAGCAT CCTCCTGACC GGAGAGGTCT TCAGCGACGA GCTCAGAGAT 
CCAGCCAGCC TCAAGTTCCA GACGCTCAGC AGAGATCTTG CGGAAAAGAT AGAAGATGCT 
CTAGAGGGGC TTCCCGGCTT TAAGAGTGTG TCTGTAATCG ACTTCAGACC ACAGAAGGAC 
TCTCAGTGGG TGGATGGCAT AGTGGTGGAT TACGCAGTCA CAGTAGCGGT GGACGGAGCC 
GGGGTGAGCT CTGAACAGCT GAACTACCTG ACCCTGCAGT CCAACCTGGT GGAGAACTCG 
TATCGAGAGG TCAAGGAGCC CCCCACAGTG GTCTACACAA TCAGCGAGAT TAAAAACATC 
TTCACCGAAC CGCATCACAT CAATGAGCTG GAGAGTGTGT CTGAGATTCC TGAGCCTGTC 
AGCGCTGCAG ACGAAGCGAC GGGCATTGAC CTATCAGAAG TCTTGACCCT GGATCATCCC 
ACTGGCGGTG GATCATCACC ACCAGTAGAA CTCAACACCC TGGGACCAGA AGAGGACATC 
GTTATTCTGG AGGAGAGTGA ACTAGCGTCA CCTGAGGCAG CACTCGCCAC CAGTCCACCT 
GAAGCTGGTA GCATTGAGGA GGAAGATCTG TTGCCAGAGG ATGTTGTGCT AGCTCCTGCT 
CCAAAGACCA TCCCGCCTAT AGAGCTCCTA CCAGAGCCTC CCTCCCARGA GACGCCAAAG 
AGCTCCACGT TGCCTGAGCC GCCAATAGAA ACAGAGGCTT CTGGGTCAGG CCGAGAAAAC 
CTGGACAGTT TAATTGAGCC ATCCATTGAA GAAGAAGAAG ACCAAATATC TCTTGTAGAG 
ACTGAAGATG TCAAATCAGA ACCTGAACAA GAAACAGTTG ATGAGGTTGA GATTTTGGAA 
AAACCTGAAG TCTCTACAGT TTTAACTAAG ACCACAATAG AACAAGGCCA GCCAGCTCTT 
TTACCCCCAA CAGAGACTGT GAATATCCCA ACTGAGGTGT CGGAGCCTGA TGTAGCCAAA 
ACCACGCTGC ACTCTGATGT TGATGAACCT GTGGTTCAGG CACCAGAGGA CTCTAGCAAG 
TTTGACAAAG ACCCTGACCA GACTGTAGGG ATCAATGTGA TTTTTACCTA TCCTGAGGAA 
CCTGCATTTG ATGAAAAAGG CTTTGACGAG ACAATTGAGA GTGATGGAAG TGAAGTGAAA 
GAGGGATCTT TGTCTGAAAC CAAGGATATA GAGGAGGAGG CACCTCTGAC CCCAGAGATC 
TTAGCAGGAG ATCTTGTGGA GGATGAGATT TTGCTCGTGA ACAGAGACAA TTCGAAGTTA 
CCCGGGACAG ACTTCCCACG CCATCCACTG CCAACTGCCC TGTCTCCAGA GGAGGAATCC 
CCTTTTACAG TCTTCTCATC CATTGTCCCA GATTTTGATG CCCTACCTGA CAAAGCCATC 
ACTTCACTGA CCACTCAGGA GGACCTGGAT GATGCTGCCC CTGAAGATCA CGGATATGAC 
CTGATTCCTT ATGATTACGG CTTGACAAAC CAGACAGAGG AAGGCAGCAC TGGATCCCCA 
TTCAGTGTGG CCCAAGGCAC AGACCAAGTC AGCATTGCCA TGCCTATAAA CCCGAGGCGA 
GCTCTGATAG TCTTCTTCAG CCTGAGAGTG ACCAACATGG TATTTTCTGA AGATCTCTTT 
AACAAGAGCT CCCCAGAGTA CAAGGCTTTG GAGCAGCGCT TCCTAGAACT GCTTGTGCCC 
TATCTTCAGT CCAACCTGAG CCACTTTGAG AATCTAGAGA TCTTGAACTT CAGGAATGGG 
AGCATCGTGG TCAACAGTCG GATGAAGTTT GGGAGGCCTG TGGCCCGGGG CGTCACCACC 
ACCGTGTATC TAATCCTGGA GGACTTCTGT AACACTGCGT ACCAGACCAT GAACCTAGCC 
ATTGATAAAT ACTCTCTGGA TGTGGAGTCA GGTGACCAAG CCGACCCCTG CAAGTTCCAG 
GCGTGCAACG AGTACGCAGA ATGTAAAGTG AACAAGTGGT CAGGGGAAGC GGAGTGCGTC 
TGTAACGCGG GCTACTTCAG TGTCGACGGC CTTCCGTGTC AAAGCATCTG TGAGCTGCAG 
ACAGACTTTT GCCTCAATGA CGGCAAGTGT GACATCATCC CTGGCCAGGG AGCCATCTGC 
AGGTGTCGTG TTGGAGAGAA CTGGTGGTAC AGAGGTGAAC ACTGTGAGGA GTATGTGTCC 
GAGCCTCTGG TGGTTGGCAT CGCCATAGCC TCTGTTGCTG GTTTCCTGTT TGTGGCGTCC 
GGAGTGCTGT TCTTCCTGGC CAGGACACTT CGGGATCAGT ACGACAAGGA AGAGTCGGAG 
GATCCCGTAC GGCGGGTGGA AAGTCTTCCG TCATTGGAAA GGGCAACCAA ATACAACCCC 
ATGTATGAAA GTGAAGCCAC TACAGGCTAC AGCCACTACT ACCGTCGTTA TCCCGAAGCG 
CCTATGTACA GCAGTGCCAG CGCCGAGGCA TCCACCGATT TTAGCAGCGA GGAGATACGG 
CACATCTATG AAAACAGCGA GCTGACCAAA GAGGAAATCC AAGACAGGAT ACGCATCATT 
GAACTCTACG CTAAGGACCG GCAGTTTGCA GACTTTGTGC GGCAGCATCA AGCAGTTCTG 
GATACCCAGA GGGAAAGCTC CTCTATGCAC TCGTAG