limd1 cDNA ORF clone, Poecilia reticulata(guppy)

Gene
Clones
gRNAs

Summary

Gene Symbol	limd1
Entrez Gene ID	103478421
Full Name	LIM domains containing 1
Gene Type	protein-coding
Organism	Poecilia reticulata(guppy)

mRNA and Protein(s)

mRNA	Protein	Name
XM_008432277.2	XP_008430499.1	LIM domain-containing protein 1

Pathways from BioSystems
Gene Ontology

The following limd1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the limd1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OPo177600	XM_008432277.2 Latest version!	Poecilia reticulata LIM domains containing 1 (limd1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

limd1 ( XM_008432277.2 ) cDNA ORF clone, Poecilia reticulata(guppy) -> XP_008430499.1 Poecilia reticulata LIM domains containing 1 (limd1), mRNA.

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CloneID	OPo177600
Clone ID Related Accession (Same CDS sequence)	XM_008432277.2
Accession Version	XM_008432277.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1716bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-20
Organism	Poecilia reticulata(guppy)
Product	LIM domain-containing protein 1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_024346.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 21, 2016 this sequence version replaced XM_008432277.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Poecilia reticulata Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATAAAT ACGAGGATTT GGGACTCGAA GCGAGTAAGT TTATCGAAGA CTTAAACATG 
TATGAAGCAT CCAGAGATGG CTTGTTTCGA ATGAGGAGAG ATGCGGGAAA TAACCCGGAC 
TTTGAAGAGA CTAGGAGGGT TTTTGCCTCC AAAATGACCA AAATACACAT GCAGAAACAG 
CAGGAGGAGA TGGCAAAAAG CAACCAGGTG ATTAAAATGA ACGGAGGTCA CCACGGCGGC 
GCTCATTACA CCAAGGACAG ACCGCCGATC AACAGCTACA GGCAGCCGGG GGAAGCGGCG 
GCGAAACCCC CGATCCTATC GGGCCCGGTG CCGAGCGCCT CGCCGGTGAA CCAGTGTGTT 
CAGGCTGAAG CTCCAGCCAG CAGGGCGCAC GGCTACGGAA GCAATTATGA TAACAACGAG 
CGCTTAGAGC CACATTCCTC CCATCACAGT AACTCTTCCA TGCCAAGTTG TGGAAGTCCA 
GCTACCAGTG CTCTGCACCC TGTCTCCCCA TCCAGAGAGG GCAAGCTGCC CCCCACTCTC 
TCAGGGAGCA GCAGTACATC CCACCATCAG CCTCAGCGCC TTCACACATC TTCCACTGTC 
AGTCCTTCAC AAGCAACCAA TCAGAGTCCA CTGTGCTCTG CTGGGACAGC CAGGGTTTGG 
ACTGGAGAGC CCATCGGTGC TCCATCATCA GAGGGCATCC CTGCCTTTCC TTTGACCGCC 
TTCACAGGTG ATGCTGACTG TCATGTGCAG CAGCCTGTGA GTCATTACTT ACAATCTGTT 
AACCACAATG GTCCTCCAGT CACTTCCTTT GCCCCAGAAA TGCCTCAGCC TAAAGCCCAG 
GTGACCTCTG CAGCCCCATG TGCCTCTAAG AACCAGCTTC CAGCTTGGAG CCAAATGCCT 
GGTGGCGTAC AGGCCATGCA GGTTATCGGT GATGGTCCTG GAGGTCCGTC CTCCCCTAAA 
CCTGTCCAGA TATCTTCTAC TGCTCAGCCC GTTCAACCGC TGGAGCAGGG GCCCTCTGCA 
GCTGAAATCA AACTAGAGGC ACTTACGAAA CAACTGGAAA AAGAGATGGA TGCCCAGCCA 
AAATCAGACT ACTTTGGGCT TTGCGTGAAG TGTAACAAGG CGGTTTATGG AGCTAACCAG 
GCCTGCCAGG CTATGGGTAG CCTCTACCAC GACACCTGCT TCACCTGCAG TGCCTGCAGT 
CGAAGGCTAA GAGGGAAGGC TTTCTACTAC GTTGGAGGGA AGGTTTTCTG TGAAGAGGAC 
TTCCTGTACT CTGGTTTCCA GCAGTCTGCT GATAAGTGTA ACGCATGTGG ACATCTGATC 
ATGGACATGA TCCTGCAGGC TCTGGGGAAG TCGTACCACC CCGGCTGTTT CCGCTGTGTG 
ATCTGCAACG AGAGCCTCGA TGGAGTTCCC TTCACTGTGG ACACGGAAAA CAAGATCTAC 
TGTGTCAAGG ACTACCACAG GGTCCTAGCT CCTAAATGTG CAGCCTGTAA CCAGCCAATC 
CTGCCCTCAG AGGGCTCTGA TGAAACCATC CGAGTCGTAT CCATGGACAA AGACTACCAT 
GTAGAATGCT ACCACTGTGA GGACTGCAAG ATGGAGCTGA ACGATGAAGA GGGTCACCGC 
TGCTACCCCC TGAACGGCCA CCTCCTCTGC CACTCCTGCC ACCTGAAGCA CATCAAGCCC 
GGCTGTCCTC CCGCAGTCCC TGCCTCCACA TACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008430499.1
CDS	569..2284
Translation	MDKYEDLGLEASKFIEDLNMYEASRDGLFRMRRDAGNNPDFEETRRVFASKMTKIHMQKQQEEMAKSNQVIKMNGGHHGGAHYTKDRPPINSYRQPGEAAAKPPILSGPVPSASPVNQCVQAEAPASRAHGYGSNYDNNERLEPHSSHHSNSSMPSCGSPATSALHPVSPSREGKLPPTLSGSSSTSHHQPQRLHTSSTVSPSQATNQSPLCSAGTARVWTGEPIGAPSSEGIPAFPLTAFTGDADCHVQQPVSHYLQSVNHNGPPVTSFAPEMPQPKAQVTSAAPCASKNQLPAWSQMPGGVQAMQVIGDGPGGPSSPKPVQISSTAQPVQPLEQGPSAAEIKLEALTKQLEKEMDAQPKSDYFGLCVKCNKAVYGANQACQAMGSLYHDTCFTCSACSRRLRGKAFYYVGGKVFCEEDFLYSGFQQSADKCNACGHLIMDMILQALGKSYHPGCFRCVICNESLDGVPFTVDTENKIYCVKDYHRVLAPKCAACNQPILPSEGSDETIRVVSMDKDYHVECYHCEDCKMELNDEEGHRCYPLNGHLLCHSCHLKHIKPGCPPAVPASTY

Target ORF information:

RefSeq Version	XM_008432277.2
Organism	Poecilia reticulata(guppy)
Definition	Poecilia reticulata LIM domains containing 1 (limd1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008432277.2

ORF Insert Sequence:

ATGGATAAAT ACGAGGATTT GGGACTCGAA GCGAGTAAGT TTATCGAAGA CTTAAACATG 
TATGAAGCAT CCAGAGATGG CTTGTTTCGA ATGAGGAGAG ATGCGGGAAA TAACCCGGAC 
TTTGAAGAGA CTAGGAGGGT TTTTGCCTCC AAAATGACCA AAATACACAT GCAGAAACAG 
CAGGAGGAGA TGGCAAAAAG CAACCAGGTG ATTAAAATGA ACGGAGGTCA CCACGGCGGC 
GCTCATTACA CCAAGGACAG ACCGCCGATC AACAGCTACA GGCAGCCGGG GGAAGCGGCG 
GCGAAACCCC CGATCCTATC GGGCCCGGTG CCGAGCGCCT CGCCGGTGAA CCAGTGTGTT 
CAGGCTGAAG CTCCAGCCAG CAGGGCGCAC GGCTACGGAA GCAATTATGA TAACAACGAG 
CGCTTAGAGC CACATTCCTC CCATCACAGT AACTCTTCCA TGCCAAGTTG TGGAAGTCCA 
GCTACCAGTG CTCTGCACCC TGTCTCCCCA TCCAGAGAGG GCAAGCTGCC CCCCACTCTC 
TCAGGGAGCA GCAGTACATC CCACCATCAG CCTCAGCGCC TTCACACATC TTCCACTGTC 
AGTCCTTCAC AAGCAACCAA TCAGAGTCCA CTGTGCTCTG CTGGGACAGC CAGGGTTTGG 
ACTGGAGAGC CCATCGGTGC TCCATCATCA GAGGGCATCC CTGCCTTTCC TTTGACCGCC 
TTCACAGGTG ATGCTGACTG TCATGTGCAG CAGCCTGTGA GTCATTACTT ACAATCTGTT 
AACCACAATG GTCCTCCAGT CACTTCCTTT GCCCCAGAAA TGCCTCAGCC TAAAGCCCAG 
GTGACCTCTG CAGCCCCATG TGCCTCTAAG AACCAGCTTC CAGCTTGGAG CCAAATGCCT 
GGTGGCGTAC AGGCCATGCA GGTTATCGGT GATGGTCCTG GAGGTCCGTC CTCCCCTAAA 
CCTGTCCAGA TATCTTCTAC TGCTCAGCCC GTTCAACCGC TGGAGCAGGG GCCCTCTGCA 
GCTGAAATCA AACTAGAGGC ACTTACGAAA CAACTGGAAA AAGAGATGGA TGCCCAGCCA 
AAATCAGACT ACTTTGGGCT TTGCGTGAAG TGTAACAAGG CGGTTTATGG AGCTAACCAG 
GCCTGCCAGG CTATGGGTAG CCTCTACCAC GACACCTGCT TCACCTGCAG TGCCTGCAGT 
CGAAGGCTAA GAGGGAAGGC TTTCTACTAC GTTGGAGGGA AGGTTTTCTG TGAAGAGGAC 
TTCCTGTACT CTGGTTTCCA GCAGTCTGCT GATAAGTGTA ACGCATGTGG ACATCTGATC 
ATGGACATGA TCCTGCAGGC TCTGGGGAAG TCGTACCACC CCGGCTGTTT CCGCTGTGTG 
ATCTGCAACG AGAGCCTCGA TGGAGTTCCC TTCACTGTGG ACACGGAAAA CAAGATCTAC 
TGTGTCAAGG ACTACCACAG GGTCCTAGCT CCTAAATGTG CAGCCTGTAA CCAGCCAATC 
CTGCCCTCAG AGGGCTCTGA TGAAACCATC CGAGTCGTAT CCATGGACAA AGACTACCAT 
GTAGAATGCT ACCACTGTGA GGACTGCAAG ATGGAGCTGA ACGATGAAGA GGGTCACCGC 
TGCTACCCCC TGAACGGCCA CCTCCTCTGC CACTCCTGCC ACCTGAAGCA CATCAAGCCC 
GGCTGTCCTC CCGCAGTCCC TGCCTCCACA TACTGA