LOC103474475 cDNA ORF clone, Poecilia reticulata(guppy)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC103474475
Entrez Gene ID	103474475
Full Name	secreted frizzled-related protein 2-like
Gene Type	protein-coding
Organism	Poecilia reticulata(guppy)

mRNA and Protein(s)

mRNA	Protein	Name
XM_008425485.2	XP_008423707.1	secreted frizzled-related protein 2-like

Pathways from BioSystems
Gene Ontology

The following LOC103474475 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC103474475 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OPo173064	XM_008425485.2 Latest version!	Poecilia reticulata secreted frizzled-related protein 2-like (LOC103474475), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$258.30 $369.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC103474475 ( XM_008425485.2 ) cDNA ORF clone, Poecilia reticulata(guppy) -> XP_008423707.1 Poecilia reticulata secreted frizzled-related protein 2-like (LOC103474475), mRNA.

Price & Availability↑

CloneID	OPo173064
Clone ID Related Accession (Same CDS sequence)	XM_008425485.2
Accession Version	XM_008425485.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1029bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-20
Organism	Poecilia reticulata(guppy)
Product	secreted frizzled-related protein 2-like
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_024343.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 21, 2016 this sequence version replaced XM_008425485.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Poecilia reticulata Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGTTT TTCTGCTCAG CTCTCTCATC ATGCTCTTCG GTGTAGTTTA TGCCTCCGAC 
CCTGCGGATC CAGTTCTGCT CGGACCTCCA ACCATCGGGT TCAGCTCTTC GGTCCGCTCG 
GTTTGTAAGC CTATCCCCAG CACACTGTCT CTGTGCCAAG GCATCGGCTA CCGGCGTATG 
TGGATCCCCA ACCTGCTCGG CCACGACTCC CTGAAAGAGG CCCAGCAGCA GTCGGCCGCC 
TGGCTGCCGC TCATCTCCAA GCTGTGCCAC CAGGACACCA AGAAGTTCCT GTGCTCGCTG 
TTCGCCCCGG TGTGCCTGCC GGAGCTCGGC GGGCCCGTGA GCCCCTGCAG GAACCTGTGC 
GAGGCCGTGC GGGACGGCTG CGTGCCCGTA ATGAGCGCCT TCGGGTTCCC CTGGCCCGAG 
ATGTTTAATT GCTCTCGGTT CCCGAGCGGG ACCGAGCTCT GTATCCCAGC AACCGGACAG 
CTGGAGGAGC GGACGGAAGA GGAGGTCAGG CGCGAGGAGG AGCTGAAAGG GAGTATCATC 
TGTGATGCCT GCTCTCTGAC TGCTGAAGGA GAGTCTGACA TCCAGGAAAA CTTCTGTCAC 
AGTCCATACG CTTTAAAGAT GCGTCTGGAC AGTGTCTCAA CAGTAGGAGG CGACCGTCAG 
CTGGTGCCTG TGGCCCGCAG CCGCATCCTG AGGTGGGCAG GGGGAGGTGC AGAGAGGGCG 
CAGGAGTTTG GAGGCGCAAT GACCCACAGG GCTCTGTGGC TGCAGGAAGG AGCCAGCTGT 
ACTTGTCCTG GCTTAGACTT GGCAGAGACA AGCAACAAGC AAGAGCAAAA TGAGGAGCCG 
GTTGACAAAA AAAAGGCAAA GAGAGGAGGA AGGCTGGAGA ACAGACCCCA AGATGGATGG 
TTCCTGGCCC TAGCTCAGGC TGAAGAAGGA AGACTCATTT TGACTCGACT CGTTAAGTGG 
ACCCGAGAGG ACAAAGAGCT AAAGAAGTTC ATCAGAAGTC TTCTGAAGAA GCCTTGTCCA 
GAGCTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008423707.1
CDS	1133..2161
Translation	MSVFLLSSLIMLFGVVYASDPADPVLLGPPTIGFSSSVRSVCKPIPSTLSLCQGIGYRRMWIPNLLGHDSLKEAQQQSAAWLPLISKLCHQDTKKFLCSLFAPVCLPELGGPVSPCRNLCEAVRDGCVPVMSAFGFPWPEMFNCSRFPSGTELCIPATGQLEERTEEEVRREEELKGSIICDACSLTAEGESDIQENFCHSPYALKMRLDSVSTVGGDRQLVPVARSRILRWAGGGAERAQEFGGAMTHRALWLQEGASCTCPGLDLAETSNKQEQNEEPVDKKKAKRGGRLENRPQDGWFLALAQAEEGRLILTRLVKWTREDKELKKFIRSLLKKPCPEL

Target ORF information:

RefSeq Version	XM_008425485.2
Organism	Poecilia reticulata(guppy)
Definition	Poecilia reticulata secreted frizzled-related protein 2-like (LOC103474475), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008425485.2

ORF Insert Sequence:

ATGTCTGTTT TTCTGCTCAG CTCTCTCATC ATGCTCTTCG GTGTAGTTTA TGCCTCCGAC 
CCTGCGGATC CAGTTCTGCT CGGACCTCCA ACCATCGGGT TCAGCTCTTC GGTCCGCTCG 
GTTTGTAAGC CTATCCCCAG CACACTGTCT CTGTGCCAAG GCATCGGCTA CCGGCGTATG 
TGGATCCCCA ACCTGCTCGG CCACGACTCC CTGAAAGAGG CCCAGCAGCA GTCGGCCGCC 
TGGCTGCCGC TCATCTCCAA GCTGTGCCAC CAGGACACCA AGAAGTTCCT GTGCTCGCTG 
TTCGCCCCGG TGTGCCTGCC GGAGCTCGGC GGGCCCGTGA GCCCCTGCAG GAACCTGTGC 
GAGGCCGTGC GGGACGGCTG CGTGCCCGTA ATGAGCGCCT TCGGGTTCCC CTGGCCCGAG 
ATGTTTAATT GCTCTCGGTT CCCGAGCGGG ACCGAGCTCT GTATCCCAGC AACCGGACAG 
CTGGAGGAGC GGACGGAAGA GGAGGTCAGG CGCGAGGAGG AGCTGAAAGG GAGTATCATC 
TGTGATGCCT GCTCTCTGAC TGCTGAAGGA GAGTCTGACA TCCAGGAAAA CTTCTGTCAC 
AGTCCATACG CTTTAAAGAT GCGTCTGGAC AGTGTCTCAA CAGTAGGAGG CGACCGTCAG 
CTGGTGCCTG TGGCCCGCAG CCGCATCCTG AGGTGGGCAG GGGGAGGTGC AGAGAGGGCG 
CAGGAGTTTG GAGGCGCAAT GACCCACAGG GCTCTGTGGC TGCAGGAAGG AGCCAGCTGT 
ACTTGTCCTG GCTTAGACTT GGCAGAGACA AGCAACAAGC AAGAGCAAAA TGAGGAGCCG 
GTTGACAAAA AAAAGGCAAA GAGAGGAGGA AGGCTGGAGA ACAGACCCCA AGATGGATGG 
TTCCTGGCCC TAGCTCAGGC TGAAGAAGGA AGACTCATTT TGACTCGACT CGTTAAGTGG 
ACCCGAGAGG ACAAAGAGCT AAAGAAGTTC ATCAGAAGTC TTCTGAAGAA GCCTTGTCCA 
GAGCTGTAA