il12b cDNA ORF clone, Poecilia reticulata(guppy)

Gene
Clones
gRNAs

Summary

Gene Symbol	il12b
Entrez Gene ID	103470881
Full Name	interleukin 12B
Gene Type	protein-coding
Organism	Poecilia reticulata(guppy)

mRNA and Protein(s)

mRNA	Protein	Name
XM_008419566.2	XP_008417788.1	interleukin-12 subunit beta

Pathways from BioSystems
Gene Ontology

The following il12b gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the il12b cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OPo169204	XM_008419566.2 Latest version!	Poecilia reticulata interleukin 12B (il12b), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$258.30 $369.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

il12b ( XM_008419566.2 ) cDNA ORF clone, Poecilia reticulata(guppy) -> XP_008417788.1 Poecilia reticulata interleukin 12B (il12b), mRNA.

Price & Availability↑

CloneID	OPo169204
Clone ID Related Accession (Same CDS sequence)	XM_008419566.2
Accession Version	XM_008419566.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1149bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-20
Organism	Poecilia reticulata(guppy)
Product	interleukin-12 subunit beta
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_024340.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 21, 2016 this sequence version replaced XM_008419566.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Poecilia reticulata Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAGAAG TTCATAAAAT GAGAGAACCG TATAAAACTG AGAGGAAGAC AGGAGAGCCA 
TCAAACCTGA GGCTTGAAAA TAAGATGAAG CTGTTTGTTG TCAGCATCCT GTGTGCATTT 
CTTCTGGTCA GTAGCCAAAA CCCAACAACT CACTGGACTC TCCAGCCAAA CGTTCTGGTG 
CTGGAAGTCG ATGGCAAGCC GGGACAACAG CCAATCAGTT GCCTGGAGAT GCCAGAGAAC 
GTTGTGACGA GCGACAACAC AAACCAGGAT ATTATTTGGA AGAAGAATGG AGTAGAGGAG 
GCGCAGAGAG GAAACTGGTA CCTGGTGCGG TTAGTGGAAA GCTTGGGAGG GGGCAACTAC 
ACGTGCCACA GCCCGGATGG AACGCTCCTA AATTACACCG AGGTCCTGAT CCAGCAAAAA 
GAAACCAAGA GACGGAGGAT TCTTGTCAAA AACCAGCAAC AGGATTATTT GAAGTGTTCC 
ACTCAGAACT ACAATGGGGA GTTTCATTGT TCTTGGACAT GGGACAGCAA ACGTGTTGGC 
AAAGCTGCAT TTATCAAAGC TCAACGCATG TCCGATGGGC AGAACAGCCA CTGCTCTGTG 
GAGCCCAGTC GCCACCAGTG GATGTGCTCG TCAGCTCAGA GCAACTTCAG CTGCTCTGTG 
GACGACGGCG GGTCCGGTAT TTCCTGCCTG GACTGGCAGC ACTGCCCGTA CGCCGAGGAG 
AGACGGCAGG TCCAGGTCAC TGTCTTTGTC AGGACCCATC AGTTTCTGCT GGAGAGCTAC 
TCTAAGCAGT TTTACTTGTC GGAAATAGTG AAACCGGACA AGGTGAGGAT TAGCAAGGTC 
AACAAAACCT TGGTAGAGTG GAGCTACCCG AGCTCGTGGA ACACGCCGTA CTCCTACTTC 
CCTCTGACGT TCCAGATCTC GCAGTTCAGC CGTCAGTGCA GAAAGTGTGA AAACCCATGC 
ACAGAGCTGA AGCATTCAAA GGCCTCCATA TTGTCTTCCT CTGACGTCTG CCAGTTTAAA 
GTGAAGCAAA AGATTAAGGT CGTGTGTGTC CGAGCGAAGG ATGCTCTCTG TGACTCAGAG 
TGGAGCGAAT GGACCCACGT CAGATTGAAA AGGGGTGAGG GAAACAGAAA GAGGAAACAG 
CAGCAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008417788.1
CDS	173..1321
Translation	MKEVHKMREPYKTERKTGEPSNLRLENKMKLFVVSILCAFLLVSSQNPTTHWTLQPNVLVLEVDGKPGQQPISCLEMPENVVTSDNTNQDIIWKKNGVEEAQRGNWYLVRLVESLGGGNYTCHSPDGTLLNYTEVLIQQKETKRRRILVKNQQQDYLKCSTQNYNGEFHCSWTWDSKRVGKAAFIKAQRMSDGQNSHCSVEPSRHQWMCSSAQSNFSCSVDDGGSGISCLDWQHCPYAEERRQVQVTVFVRTHQFLLESYSKQFYLSEIVKPDKVRISKVNKTLVEWSYPSSWNTPYSYFPLTFQISQFSRQCRKCENPCTELKHSKASILSSSDVCQFKVKQKIKVVCVRAKDALCDSEWSEWTHVRLKRGEGNRKRKQQQ

Target ORF information:

RefSeq Version	XM_008419566.2
Organism	Poecilia reticulata(guppy)
Definition	Poecilia reticulata interleukin 12B (il12b), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008419566.2

ORF Insert Sequence:

ATGAAAGAAG TTCATAAAAT GAGAGAACCG TATAAAACTG AGAGGAAGAC AGGAGAGCCA 
TCAAACCTGA GGCTTGAAAA TAAGATGAAG CTGTTTGTTG TCAGCATCCT GTGTGCATTT 
CTTCTGGTCA GTAGCCAAAA CCCAACAACT CACTGGACTC TCCAGCCAAA CGTTCTGGTG 
CTGGAAGTCG ATGGCAAGCC GGGACAACAG CCAATCAGTT GCCTGGAGAT GCCAGAGAAC 
GTTGTGACGA GCGACAACAC AAACCAGGAT ATTATTTGGA AGAAGAATGG AGTAGAGGAG 
GCGCAGAGAG GAAACTGGTA CCTGGTGCGG TTAGTGGAAA GCTTGGGAGG GGGCAACTAC 
ACGTGCCACA GCCCGGATGG AACGCTCCTA AATTACACCG AGGTCCTGAT CCAGCAAAAA 
GAAACCAAGA GACGGAGGAT TCTTGTCAAA AACCAGCAAC AGGATTATTT GAAGTGTTCC 
ACTCAGAACT ACAATGGGGA GTTTCATTGT TCTTGGACAT GGGACAGCAA ACGTGTTGGC 
AAAGCTGCAT TTATCAAAGC TCAACGCATG TCCGATGGGC AGAACAGCCA CTGCTCTGTG 
GAGCCCAGTC GCCACCAGTG GATGTGCTCG TCAGCTCAGA GCAACTTCAG CTGCTCTGTG 
GACGACGGCG GGTCCGGTAT TTCCTGCCTG GACTGGCAGC ACTGCCCGTA CGCCGAGGAG 
AGACGGCAGG TCCAGGTCAC TGTCTTTGTC AGGACCCATC AGTTTCTGCT GGAGAGCTAC 
TCTAAGCAGT TTTACTTGTC GGAAATAGTG AAACCGGACA AGGTGAGGAT TAGCAAGGTC 
AACAAAACCT TGGTAGAGTG GAGCTACCCG AGCTCGTGGA ACACGCCGTA CTCCTACTTC 
CCTCTGACGT TCCAGATCTC GCAGTTCAGC CGTCAGTGCA GAAAGTGTGA AAACCCATGC 
ACAGAGCTGA AGCATTCAAA GGCCTCCATA TTGTCTTCCT CTGACGTCTG CCAGTTTAAA 
GTGAAGCAAA AGATTAAGGT CGTGTGTGTC CGAGCGAAGG ATGCTCTCTG TGACTCAGAG 
TGGAGCGAAT GGACCCACGT CAGATTGAAA AGGGGTGAGG GAAACAGAAA GAGGAAACAG 
CAGCAATGA