cdc27 cDNA ORF clone, Poecilia reticulata(guppy)

Gene
Clones
gRNAs

Summary

Gene Symbol	cdc27
Entrez Gene ID	103469031
Full Name	cell division cycle 27
Gene Type	protein-coding
Organism	Poecilia reticulata(guppy)

mRNA and Protein(s)

mRNA	Protein	Name
XM_008416501.2	XP_008414723.1	cell division cycle protein 27 homolog isoform X2
XM_017306416.1	XP_017161905.1	cell division cycle protein 27 homolog isoform X1

Pathways from BioSystems
Gene Ontology

The following cdc27 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the cdc27 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OPo200628	XM_017306416.1 Latest version!	Poecilia reticulata cell division cycle 27 (cdc27), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
OPo200629	XM_008416501.2 Latest version!	Poecilia reticulata cell division cycle 27 (cdc27), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

cdc27 ( XM_017306416.1 ) cDNA ORF clone, Poecilia reticulata(guppy) -> XP_017161905.1 Poecilia reticulata cell division cycle 27 (cdc27), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OPo200628
Clone ID Related Accession (Same CDS sequence)	XM_017306416.1
Accession Version	XM_017306416.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2436bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-20
Organism	Poecilia reticulata(guppy)
Product	cell division cycle protein 27 homolog isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_024338.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Poecilia reticulata Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGGTGC TGCAGGAACC TGTCCAGGCT GCGGTGTGGC AGGCTCTCAA CCACTATGCC 
TACCTGGATG CTGTTTTCCT TGCAGAGAGA CTCTACGCAG AAGTGCGTTC AGAGGAGGCC 
CTTTACCTGT TGGCTACCTG TTATTACCGC TCTGGGAAAC CGTACAAAGC TTACCGACTA 
CTGAAGGCTC ACAGCTGCTC CACGCCGCAG GTTCGATTCC TGCTGGCTAA GTGCTGCGTG 
GAACTCAGCA AGCTCGCTGA GGGAGAGCAG GTTCTGATTG GTGGAGTCCT GAACAAACAG 
AAGAGTCAGG ATGACATCAT CACTGAGTTT GGAGACTCCG CCTCCTTCAC GCTGTCGTTA 
CTGGGCCACA TTTACTGCAA GACGGACCGA GCTGCTAAGG GTGCCGAGTG CTTCCAGAGG 
AGTTTAACCC TCAACCCGTT CCTCTGGTCG CCGTTCCAGA ACCTCTGTCA TCTAGGAGAA 
AAGCCGGATC CAGATCAGGT TTTCAGGTTG TCGGCTTTGC AGAACTCCTC TTTAGCCCCG 
CCTCTTCCTC ACATAAGCCC CGCCCAGAAC CCGTCTCATC GCCTCGACAC TGCACTGATG 
GAAACGCCGC AGGACACACT GGAGTTGAAT CGTCTGAATC TGGAGTCGTC AAACGGGAAG 
CTGACGTCTG ATTTGTCCAT TTCATACATT GACTCCTCCC TCATCTCCCC GGAAACCGGC 
TCGTTATTGG GCAACACTGT TTCCATGGCA TCAGCTGGCA CTCTGCTGGC TAAACAGAAC 
AAACCCAAGA GTGGGCGGAG CTTACTGGGG GGTCCTGCCG GCCTCAGCCC CCTGACGCCC 
AGTTTCGGCA TTCTGCCCCT GGAGCCCAGC CCCGGAGACC CCACGTATTT GCAGAACTAT 
TCGGCTACAA TGGAAACGCA AACGACAGCT CCCAGCAAGA AGGCCGTCTC CAGGATCAGT 
CAGTCCAAGT CCGTCTTCAG TCAGAGCGGG AACAGCAGAG ACGTTCTTCC CATCCCCTTC 
AACCAATCGC AGACCTCAGC ACCTCACACC AGTGGTTCTC CTCAGGTTCT CAGTCCCAGT 
ATGTCTGGTC CTCCTAACGT TCAGCCCAGA AGAAGCTCCA GACTGTTTAC CAGCGCCAGC 
TCCACTGCCA AGGAGAACAG CAAGAAGCTA AAGATGAAGT TTCCTACGAA GATTCCCAAC 
CGGAAAACCA AATGTAAATC TGCGAAGACG GCGAACAGCA GCAACCTGAA CGAGAGTCTG 
GACATCCTGA GGCTGGATCC CAGTTTGAAC CTGCCAGACG CCAGGATGCC CCAGTACCAG 
AGGGCCGCTG CAGACGGCGT GATGGCGTTG CTACGGGAAC TGGGGCGGGG CTACCAGGCG 
CTGTGCTCCT ACAGCTGCAG AGAGGCCATC AACATCCTGA TGTCTCTGCC CCCTCAGCAT 
TACAACACGG GCTGGGTCCT GACACACGTC GGCCGCGCCT ACTTCGAGCT GGCCGAGTAC 
ATGCAGGCGG AGCGGCTGTT CAGCGAGGTG CGCAGGATCG AGTCGTACCG CGTGGAAGGC 
ATGGAGATCT ACTCCACCAC GCTGTGGCAC CTGCAGAAGG ACGTGGCGCT GTCGGCGCTG 
GCCAAGGACC TGACGGACAT GGACAAGAAC TGTCCTGAGG CCTGGTGCGT CGCAGGAAAC 
TGTTTCAGCC TGCAGAGGGA GCACGACATC GCCATCAAGT TCTTCCAGAG GGCCATCCAG 
GTGGACCCGG GGTTTGCGTA CGCCTACACC CTGCTGGGTC ACGAGTTTGT TCTGACTGAG 
GAGTTGGACC GAGCTCTGGC CTGTTTCAGG AACGCCATCA GAGTGAACAA CCGACACTAC 
AATGCCTGGT ATGGACTCGG CATGATTTAC TATAAACAGG AGAAGTTCAA CTTGGCAGAA 
ATCCACTTCA AGAAAGCTCT GAGCATCAAC CCTCAGAGCT CCGTGCTGCT CTGCCACATT 
GGAGTGGTGC AGCATGCTCT GAAGAAGTCT GACGCTGCGT TAGAGACTCT AAACAGAGCC 
ATTGGCATCG ACCCCAAAAA CCCGCTCTGC AAGTTCCACA GAGCCTCCAT CTTGTTTGCC 
AACGATAAAT ACAAGGCGGC CCTTCAGGAG CTGGAGGAGT TGAAGCAGAT TGTTCCCAAA 
GAGTCTCTGG TTTACTTCCT CATAGGAAAG GTGTATAAAA AGCTGGGTCA GACCCACCTG 
GCTCTGATGA ACTTCAGCTG GGCCATGGAT CTGGACCCTA AAGGAGCCAA TAATCAGATA 
AAAGAAGCCA TTGATAAGCG ATACCTGCCT GAGGATGAAG GTACGGTTTG CTCCACCTGT 
CTCTCTCACT CGTCTGTCCC TCTGACTCAC CCGCCCCTCC CCTCCCTGTT TCTGCAGTGG 
CTGAGGTGGA TGACAGTCAG GACAGCAGCT TCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017161905.1
CDS	141..2576
Translation	MTVLQEPVQAAVWQALNHYAYLDAVFLAERLYAEVRSEEALYLLATCYYRSGKPYKAYRLLKAHSCSTPQVRFLLAKCCVELSKLAEGEQVLIGGVLNKQKSQDDIITEFGDSASFTLSLLGHIYCKTDRAAKGAECFQRSLTLNPFLWSPFQNLCHLGEKPDPDQVFRLSALQNSSLAPPLPHISPAQNPSHRLDTALMETPQDTLELNRLNLESSNGKLTSDLSISYIDSSLISPETGSLLGNTVSMASAGTLLAKQNKPKSGRSLLGGPAGLSPLTPSFGILPLEPSPGDPTYLQNYSATMETQTTAPSKKAVSRISQSKSVFSQSGNSRDVLPIPFNQSQTSAPHTSGSPQVLSPSMSGPPNVQPRRSSRLFTSASSTAKENSKKLKMKFPTKIPNRKTKCKSAKTANSSNLNESLDILRLDPSLNLPDARMPQYQRAAADGVMALLRELGRGYQALCSYSCREAINILMSLPPQHYNTGWVLTHVGRAYFELAEYMQAERLFSEVRRIESYRVEGMEIYSTTLWHLQKDVALSALAKDLTDMDKNCPEAWCVAGNCFSLQREHDIAIKFFQRAIQVDPGFAYAYTLLGHEFVLTEELDRALACFRNAIRVNNRHYNAWYGLGMIYYKQEKFNLAEIHFKKALSINPQSSVLLCHIGVVQHALKKSDAALETLNRAIGIDPKNPLCKFHRASILFANDKYKAALQELEELKQIVPKESLVYFLIGKVYKKLGQTHLALMNFSWAMDLDPKGANNQIKEAIDKRYLPEDEGTVCSTCLSHSSVPLTHPPLPSLFLQWLRWMTVRTAAS

Target ORF information:

RefSeq Version	XM_017306416.1
Organism	Poecilia reticulata(guppy)
Definition	Poecilia reticulata cell division cycle 27 (cdc27), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017306416.1

ORF Insert Sequence:

ATGACGGTGC TGCAGGAACC TGTCCAGGCT GCGGTGTGGC AGGCTCTCAA CCACTATGCC 
TACCTGGATG CTGTTTTCCT TGCAGAGAGA CTCTACGCAG AAGTGCGTTC AGAGGAGGCC 
CTTTACCTGT TGGCTACCTG TTATTACCGC TCTGGGAAAC CGTACAAAGC TTACCGACTA 
CTGAAGGCTC ACAGCTGCTC CACGCCGCAG GTTCGATTCC TGCTGGCTAA GTGCTGCGTG 
GAACTCAGCA AGCTCGCTGA GGGAGAGCAG GTTCTGATTG GTGGAGTCCT GAACAAACAG 
AAGAGTCAGG ATGACATCAT CACTGAGTTT GGAGACTCCG CCTCCTTCAC GCTGTCGTTA 
CTGGGCCACA TTTACTGCAA GACGGACCGA GCTGCTAAGG GTGCCGAGTG CTTCCAGAGG 
AGTTTAACCC TCAACCCGTT CCTCTGGTCG CCGTTCCAGA ACCTCTGTCA TCTAGGAGAA 
AAGCCGGATC CAGATCAGGT TTTCAGGTTG TCGGCTTTGC AGAACTCCTC TTTAGCCCCG 
CCTCTTCCTC ACATAAGCCC CGCCCAGAAC CCGTCTCATC GCCTCGACAC TGCACTGATG 
GAAACGCCGC AGGACACACT GGAGTTGAAT CGTCTGAATC TGGAGTCGTC AAACGGGAAG 
CTGACGTCTG ATTTGTCCAT TTCATACATT GACTCCTCCC TCATCTCCCC GGAAACCGGC 
TCGTTATTGG GCAACACTGT TTCCATGGCA TCAGCTGGCA CTCTGCTGGC TAAACAGAAC 
AAACCCAAGA GTGGGCGGAG CTTACTGGGG GGTCCTGCCG GCCTCAGCCC CCTGACGCCC 
AGTTTCGGCA TTCTGCCCCT GGAGCCCAGC CCCGGAGACC CCACGTATTT GCAGAACTAT 
TCGGCTACAA TGGAAACGCA AACGACAGCT CCCAGCAAGA AGGCCGTCTC CAGGATCAGT 
CAGTCCAAGT CCGTCTTCAG TCAGAGCGGG AACAGCAGAG ACGTTCTTCC CATCCCCTTC 
AACCAATCGC AGACCTCAGC ACCTCACACC AGTGGTTCTC CTCAGGTTCT CAGTCCCAGT 
ATGTCTGGTC CTCCTAACGT TCAGCCCAGA AGAAGCTCCA GACTGTTTAC CAGCGCCAGC 
TCCACTGCCA AGGAGAACAG CAAGAAGCTA AAGATGAAGT TTCCTACGAA GATTCCCAAC 
CGGAAAACCA AATGTAAATC TGCGAAGACG GCGAACAGCA GCAACCTGAA CGAGAGTCTG 
GACATCCTGA GGCTGGATCC CAGTTTGAAC CTGCCAGACG CCAGGATGCC CCAGTACCAG 
AGGGCCGCTG CAGACGGCGT GATGGCGTTG CTACGGGAAC TGGGGCGGGG CTACCAGGCG 
CTGTGCTCCT ACAGCTGCAG AGAGGCCATC AACATCCTGA TGTCTCTGCC CCCTCAGCAT 
TACAACACGG GCTGGGTCCT GACACACGTC GGCCGCGCCT ACTTCGAGCT GGCCGAGTAC 
ATGCAGGCGG AGCGGCTGTT CAGCGAGGTG CGCAGGATCG AGTCGTACCG CGTGGAAGGC 
ATGGAGATCT ACTCCACCAC GCTGTGGCAC CTGCAGAAGG ACGTGGCGCT GTCGGCGCTG 
GCCAAGGACC TGACGGACAT GGACAAGAAC TGTCCTGAGG CCTGGTGCGT CGCAGGAAAC 
TGTTTCAGCC TGCAGAGGGA GCACGACATC GCCATCAAGT TCTTCCAGAG GGCCATCCAG 
GTGGACCCGG GGTTTGCGTA CGCCTACACC CTGCTGGGTC ACGAGTTTGT TCTGACTGAG 
GAGTTGGACC GAGCTCTGGC CTGTTTCAGG AACGCCATCA GAGTGAACAA CCGACACTAC 
AATGCCTGGT ATGGACTCGG CATGATTTAC TATAAACAGG AGAAGTTCAA CTTGGCAGAA 
ATCCACTTCA AGAAAGCTCT GAGCATCAAC CCTCAGAGCT CCGTGCTGCT CTGCCACATT 
GGAGTGGTGC AGCATGCTCT GAAGAAGTCT GACGCTGCGT TAGAGACTCT AAACAGAGCC 
ATTGGCATCG ACCCCAAAAA CCCGCTCTGC AAGTTCCACA GAGCCTCCAT CTTGTTTGCC 
AACGATAAAT ACAAGGCGGC CCTTCAGGAG CTGGAGGAGT TGAAGCAGAT TGTTCCCAAA 
GAGTCTCTGG TTTACTTCCT CATAGGAAAG GTGTATAAAA AGCTGGGTCA GACCCACCTG 
GCTCTGATGA ACTTCAGCTG GGCCATGGAT CTGGACCCTA AAGGAGCCAA TAATCAGATA 
AAAGAAGCCA TTGATAAGCG ATACCTGCCT GAGGATGAAG GTACGGTTTG CTCCACCTGT 
CTCTCTCACT CGTCTGTCCC TCTGACTCAC CCGCCCCTCC CCTCCCTGTT TCTGCAGTGG 
CTGAGGTGGA TGACAGTCAG GACAGCAGCT TCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cdc27 ( XM_008416501.2 ) cDNA ORF clone, Poecilia reticulata(guppy) -> XP_008414723.1 Poecilia reticulata cell division cycle 27 (cdc27), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OPo200629
Clone ID Related Accession (Same CDS sequence)	XM_008416501.2
Accession Version	XM_008416501.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2412bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-20
Organism	Poecilia reticulata(guppy)
Product	cell division cycle protein 27 homolog isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_024338.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 21, 2016 this sequence version replaced XM_008416501.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Poecilia reticulata Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGGTGC TGCAGGAACC TGTCCAGGCT GCGGTGTGGC AGGCTCTCAA CCACTATGCC 
TACCTGGATG CTGTTTTCCT TGCAGAGAGA CTCTACGCAG AAGTGCGTTC AGAGGAGGCC 
CTTTACCTGT TGGCTACCTG TTATTACCGC TCTGGGAAAC CGTACAAAGC TTACCGACTA 
CTGAAGGCTC ACAGCTGCTC CACGCCGCAG GTTCGATTCC TGCTGGCTAA GTGCTGCGTG 
GAACTCAGCA AGCTCGCTGA GGGAGAGCAG GTTCTGATTG GTGGAGTCCT GAACAAACAG 
AAGAGTCAGG ATGACATCAT CACTGAGTTT GGAGACTCCG CCTCCTTCAC GCTGTCGTTA 
CTGGGCCACA TTTACTGCAA GACGGACCGA GCTGCTAAGG GTGCCGAGTG CTTCCAGAGG 
AGTTTAACCC TCAACCCGTT CCTCTGGTCG CCGTTCCAGA ACCTCTGTCA TCTAGGAGAA 
AAGCCGGATC CAGATCAGGT TTTCAGGTTG TCGGCTTTGC AGAACTCCTC TTTAGCCCCG 
CCTCTTCCTC ACATAAGCCC CGCCCAGAAC CCGTCTCATC GCCTCGACAC TGCACTGATG 
GAAACGCCGC AGGACACACT GGAGTTGAAT CGTCTGAATC TGGAGTCGTC AAACGGGAAG 
CTGACGTCTG ATTTGTCCAT TTCATACATT GACTCCTCCC TCATCTCCCC GGAAACCGGC 
TCGTTATTGG GCAACACTGT TTCCATGGCA TCAGCTGGCA CTCTGCTGGC TAAACAGAAC 
AAACCCAAGA GTGGGCGGAG CTTACTGGGG GGTCCTGCCG GCCTCAGCCC CCTGACGCCC 
AGTTTCGGCA TTCTGCCCCT GGAGCCCAGC CCCGGAGACC CCACGTATTT GCAGAACTAT 
TCGGCTACAA TGGAAACGCA AACGACAGCT CCCAGCAAGA AGGCCGTCTC CAGGATCAGT 
CAGTCCAAGT CCGTCTTCAG TCAGAGCGGG AACAGCAGAG ACGTTCTTCC CATCCCCTTC 
AACCAATCGC AGACCTCAGC ACCTCACACC AGTGGTTCTC CTCAGGTTCT CAGTCCCAGT 
ATGTCTGGTC CTCCTAACGT TCAGCCCAGA AGAAGCTCCA GACTGTTTAC CAGCGCCAGC 
TCCACTGCCA AGGAGAACAG CAAGAAGCTA AAGATGAAGT TTCCTACGAA GATTCCCAAC 
CGGAAAACCA AATGTAAATC TGCGAAGACG GCGAACAGCA GCAACCTGAA CGAGAGTCTG 
GACATCCTGA GGCTGGATCC CAGTTTGAAC CTGCCAGACG CCAGGATGCC CCAGTACCAG 
AGGGCCGCTG CAGACGGCGT GATGGCGTTG CTACGGGAAC TGGGGCGGGG CTACCAGGCG 
CTGTGCTCCT ACAGCTGCAG AGAGGCCATC AACATCCTGA TGTCTCTGCC CCCTCAGCAT 
TACAACACGG GCTGGGTCCT GACACACGTC GGCCGCGCCT ACTTCGAGCT GGCCGAGTAC 
ATGCAGGCGG AGCGGCTGTT CAGCGAGGTG CGCAGGATCG AGTCGTACCG CGTGGAAGGC 
ATGGAGATCT ACTCCACCAC GCTGTGGCAC CTGCAGAAGG ACGTGGCGCT GTCGGCGCTG 
GCCAAGGACC TGACGGACAT GGACAAGAAC TGTCCTGAGG CCTGGTGCGT CGCAGGAAAC 
TGTTTCAGCC TGCAGAGGGA GCACGACATC GCCATCAAGT TCTTCCAGAG GGCCATCCAG 
GTGGACCCGG GGTTTGCGTA CGCCTACACC CTGCTGGGTC ACGAGTTTGT TCTGACTGAG 
GAGTTGGACC GAGCTCTGGC CTGTTTCAGG AACGCCATCA GAGTGAACAA CCGACACTAC 
AATGCCTGGT ATGGACTCGG CATGATTTAC TATAAACAGG AGAAGTTCAA CTTGGCAGAA 
ATCCACTTCA AGAAAGCTCT GAGCATCAAC CCTCAGAGCT CCGTGCTGCT CTGCCACATT 
GGAGTGGTGC AGCATGCTCT GAAGAAGTCT GACGCTGCGT TAGAGACTCT AAACAGAGCC 
ATTGGCATCG ACCCCAAAAA CCCGCTCTGC AAGTTCCACA GAGCCTCCAT CTTGTTTGCC 
AACGATAAAT ACAAGGCGGC CCTTCAGGAG CTGGAGGAGT TGAAGCAGAT TGTTCCCAAA 
GAGTCTCTGG TTTACTTCCT CATAGGAAAG GTGTATAAAA AGCTGGGTCA GACCCACCTG 
GCTCTGATGA ACTTCAGCTG GGCCATGGAT CTGGACCCTA AAGGAGCCAA TAATCAGATA 
AAAGAAGCCA TTGATAAGCG ATACCTGCCT GAGGATGAAG TGGCTGAGGT GGATGACAGT 
CAGGACAGCA GCTTCATGAC GGACGCCGAC GACACGCAGC TCCACACGCC AGACAGCGAT 
GATGTTCTTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008414723.1
CDS	139..2550
Translation	MTVLQEPVQAAVWQALNHYAYLDAVFLAERLYAEVRSEEALYLLATCYYRSGKPYKAYRLLKAHSCSTPQVRFLLAKCCVELSKLAEGEQVLIGGVLNKQKSQDDIITEFGDSASFTLSLLGHIYCKTDRAAKGAECFQRSLTLNPFLWSPFQNLCHLGEKPDPDQVFRLSALQNSSLAPPLPHISPAQNPSHRLDTALMETPQDTLELNRLNLESSNGKLTSDLSISYIDSSLISPETGSLLGNTVSMASAGTLLAKQNKPKSGRSLLGGPAGLSPLTPSFGILPLEPSPGDPTYLQNYSATMETQTTAPSKKAVSRISQSKSVFSQSGNSRDVLPIPFNQSQTSAPHTSGSPQVLSPSMSGPPNVQPRRSSRLFTSASSTAKENSKKLKMKFPTKIPNRKTKCKSAKTANSSNLNESLDILRLDPSLNLPDARMPQYQRAAADGVMALLRELGRGYQALCSYSCREAINILMSLPPQHYNTGWVLTHVGRAYFELAEYMQAERLFSEVRRIESYRVEGMEIYSTTLWHLQKDVALSALAKDLTDMDKNCPEAWCVAGNCFSLQREHDIAIKFFQRAIQVDPGFAYAYTLLGHEFVLTEELDRALACFRNAIRVNNRHYNAWYGLGMIYYKQEKFNLAEIHFKKALSINPQSSVLLCHIGVVQHALKKSDAALETLNRAIGIDPKNPLCKFHRASILFANDKYKAALQELEELKQIVPKESLVYFLIGKVYKKLGQTHLALMNFSWAMDLDPKGANNQIKEAIDKRYLPEDEVAEVDDSQDSSFMTDADDTQLHTPDSDDVL

Target ORF information:

RefSeq Version	XM_008416501.2
Organism	Poecilia reticulata(guppy)
Definition	Poecilia reticulata cell division cycle 27 (cdc27), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008416501.2

ORF Insert Sequence:

ATGACGGTGC TGCAGGAACC TGTCCAGGCT GCGGTGTGGC AGGCTCTCAA CCACTATGCC 
TACCTGGATG CTGTTTTCCT TGCAGAGAGA CTCTACGCAG AAGTGCGTTC AGAGGAGGCC 
CTTTACCTGT TGGCTACCTG TTATTACCGC TCTGGGAAAC CGTACAAAGC TTACCGACTA 
CTGAAGGCTC ACAGCTGCTC CACGCCGCAG GTTCGATTCC TGCTGGCTAA GTGCTGCGTG 
GAACTCAGCA AGCTCGCTGA GGGAGAGCAG GTTCTGATTG GTGGAGTCCT GAACAAACAG 
AAGAGTCAGG ATGACATCAT CACTGAGTTT GGAGACTCCG CCTCCTTCAC GCTGTCGTTA 
CTGGGCCACA TTTACTGCAA GACGGACCGA GCTGCTAAGG GTGCCGAGTG CTTCCAGAGG 
AGTTTAACCC TCAACCCGTT CCTCTGGTCG CCGTTCCAGA ACCTCTGTCA TCTAGGAGAA 
AAGCCGGATC CAGATCAGGT TTTCAGGTTG TCGGCTTTGC AGAACTCCTC TTTAGCCCCG 
CCTCTTCCTC ACATAAGCCC CGCCCAGAAC CCGTCTCATC GCCTCGACAC TGCACTGATG 
GAAACGCCGC AGGACACACT GGAGTTGAAT CGTCTGAATC TGGAGTCGTC AAACGGGAAG 
CTGACGTCTG ATTTGTCCAT TTCATACATT GACTCCTCCC TCATCTCCCC GGAAACCGGC 
TCGTTATTGG GCAACACTGT TTCCATGGCA TCAGCTGGCA CTCTGCTGGC TAAACAGAAC 
AAACCCAAGA GTGGGCGGAG CTTACTGGGG GGTCCTGCCG GCCTCAGCCC CCTGACGCCC 
AGTTTCGGCA TTCTGCCCCT GGAGCCCAGC CCCGGAGACC CCACGTATTT GCAGAACTAT 
TCGGCTACAA TGGAAACGCA AACGACAGCT CCCAGCAAGA AGGCCGTCTC CAGGATCAGT 
CAGTCCAAGT CCGTCTTCAG TCAGAGCGGG AACAGCAGAG ACGTTCTTCC CATCCCCTTC 
AACCAATCGC AGACCTCAGC ACCTCACACC AGTGGTTCTC CTCAGGTTCT CAGTCCCAGT 
ATGTCTGGTC CTCCTAACGT TCAGCCCAGA AGAAGCTCCA GACTGTTTAC CAGCGCCAGC 
TCCACTGCCA AGGAGAACAG CAAGAAGCTA AAGATGAAGT TTCCTACGAA GATTCCCAAC 
CGGAAAACCA AATGTAAATC TGCGAAGACG GCGAACAGCA GCAACCTGAA CGAGAGTCTG 
GACATCCTGA GGCTGGATCC CAGTTTGAAC CTGCCAGACG CCAGGATGCC CCAGTACCAG 
AGGGCCGCTG CAGACGGCGT GATGGCGTTG CTACGGGAAC TGGGGCGGGG CTACCAGGCG 
CTGTGCTCCT ACAGCTGCAG AGAGGCCATC AACATCCTGA TGTCTCTGCC CCCTCAGCAT 
TACAACACGG GCTGGGTCCT GACACACGTC GGCCGCGCCT ACTTCGAGCT GGCCGAGTAC 
ATGCAGGCGG AGCGGCTGTT CAGCGAGGTG CGCAGGATCG AGTCGTACCG CGTGGAAGGC 
ATGGAGATCT ACTCCACCAC GCTGTGGCAC CTGCAGAAGG ACGTGGCGCT GTCGGCGCTG 
GCCAAGGACC TGACGGACAT GGACAAGAAC TGTCCTGAGG CCTGGTGCGT CGCAGGAAAC 
TGTTTCAGCC TGCAGAGGGA GCACGACATC GCCATCAAGT TCTTCCAGAG GGCCATCCAG 
GTGGACCCGG GGTTTGCGTA CGCCTACACC CTGCTGGGTC ACGAGTTTGT TCTGACTGAG 
GAGTTGGACC GAGCTCTGGC CTGTTTCAGG AACGCCATCA GAGTGAACAA CCGACACTAC 
AATGCCTGGT ATGGACTCGG CATGATTTAC TATAAACAGG AGAAGTTCAA CTTGGCAGAA 
ATCCACTTCA AGAAAGCTCT GAGCATCAAC CCTCAGAGCT CCGTGCTGCT CTGCCACATT 
GGAGTGGTGC AGCATGCTCT GAAGAAGTCT GACGCTGCGT TAGAGACTCT AAACAGAGCC 
ATTGGCATCG ACCCCAAAAA CCCGCTCTGC AAGTTCCACA GAGCCTCCAT CTTGTTTGCC 
AACGATAAAT ACAAGGCGGC CCTTCAGGAG CTGGAGGAGT TGAAGCAGAT TGTTCCCAAA 
GAGTCTCTGG TTTACTTCCT CATAGGAAAG GTGTATAAAA AGCTGGGTCA GACCCACCTG 
GCTCTGATGA ACTTCAGCTG GGCCATGGAT CTGGACCCTA AAGGAGCCAA TAATCAGATA 
AAAGAAGCCA TTGATAAGCG ATACCTGCCT GAGGATGAAG TGGCTGAGGT GGATGACAGT 
CAGGACAGCA GCTTCATGAC GGACGCCGAC GACACGCAGC TCCACACGCC AGACAGCGAT 
GATGTTCTTT AG