sema3d cDNA ORF clone, Poecilia reticulata(guppy)

Gene
Clones
gRNAs

Summary

Gene Symbol	sema3d
Entrez Gene ID	103466345
Full Name	semaphorin 3D
Gene Type	protein-coding
Organism	Poecilia reticulata(guppy)

mRNA and Protein(s)

mRNA	Protein	Name
XM_017305161.1	XP_017160650.1	semaphorin-3D

Pathways from BioSystems
Gene Ontology

The following sema3d gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the sema3d cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OPo195988	XM_017305161.1 Latest version!	Poecilia reticulata semaphorin 3D (sema3d), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

sema3d ( XM_017305161.1 ) cDNA ORF clone, Poecilia reticulata(guppy) -> XP_017160650.1 Poecilia reticulata semaphorin 3D (sema3d), mRNA.

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CloneID	OPo195988
Clone ID Related Accession (Same CDS sequence)	XM_017305161.1
Accession Version	XM_017305161.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2436bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-20
Organism	Poecilia reticulata(guppy)
Product	semaphorin-3D
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_024336.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Poecilia reticulata Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 1% of CDS bases ##RefSeq-Attributes-END##

ATGGCAACAG GAGACTCTGG AAGTAAGAGG AAGAATAAAG CTTGGACTCT GTTTTGCTTG 
CCTGTCTGTA ATTATGGCAG AGAAGGACGA AGGACTTTTG TTTCGTTCCA TCCACTGGTT 
CTGTTTGTGG GGCTGATTTT GTTGGCCAGC CTTCCTTTTG GAGCGAGCAT CAAACAGAGT 
GTCCCCAGAG TCAAACTCAG CTACAGAGAA TTGCTTCAGG CTGGAAGTAT CTATCTGTTT 
GCGGGCCCTG CCGATGGCCT GCACTCCCAC TCTCTGCTGC TGGATGAGGA GAGAGGCAGT 
CTTTTGCTCG GAGCTAGGGA TCACATCTAC CTGCTTGACC CGGACAATGT GGGCAAAACC 
CCAAGGAAGA TCCACTGGCC TGCTCTGAGA GACAGAGTTG AAACATGCAA ACTTGCTGGG 
AAAAGTACAA ATTTGGAATG TGCTAACTTT GTAAGAGTCC TCCACAATTA CAACCGGACA 
CATGTCTACG CCTGTGGGAC AGGAGCCTTC CACCCCACCT GTGCTTTCAT TGAAATTTCT 
GGGCAGAGAG AGGATGGTGT TTTCCAACTG TTGCCTAATA CAGCAGATTC TGGCAGGTTG 
AAGTGTCCTT TCGATCCGTT GCAGCCGTTC ACCTCAGTCC TTTCAGATCA GTACCTGTAT 
GTGGGCACAT CATCAGACTT TCTGGGAAAA GACACTACGT TCACGCGCTC CTTAGGTCCT 
CCGCCTGACC AGCACTACAT ACGCACAGAC ATCTCTGAAG ACTACTGGAT CAACGCCTCA 
CCGCAGACAC AAAGAGCCGC CTGTAGCCTC AGGATGATTA TTGGCATTCC ACAGAAAAGG 
AAACAGTCTA CTTATTCATT TCACTGGAAC TTTTATATGA ATGCCACAAC TGAACGGGGG 
CCACACCTAC AGGGGAATTT ACATGTGTTT CAGAATGATG TCGGCGGGCT TCGGAGTCTA 
ACCAATAAGT GGACCACCTT CCTCAAAGCC AGGCTTGTGT GTTCAATTCC AGGACCAGAT 
GGTGTGGACA CACACTTTGA TGAGCTCCAG GACATCTTTC TGCTTCCTAC CAGAGATGAA 
AAAAACCCCA TAGTGTATGC AGTCTTCTCT ACTTCCAGTT CCATTTTCCG GGGCTCAGCA 
GTATGTGTAT ATAGCATGGC AGACATAAGG GCGGCGTTCA ATGGACCATA CGCCCACAAA 
GAGGGGCCCG ACCACAGATG GGTGGAATAY GAGGGGAGAA TACCATATCC CCGTCCTGGA 
ACGTGCCCCA GTAAGACGTA TGATCCAAGA ATCAAAACCA CCAAAGACTT TCCAGATGAG 
GTGATCAGTT TTATTCGATT CCACCCACTG ATGCACCACT CAGTTTACCC TCTGGCTGGT 
CGACCGGTCT TCACACGTGT CCGAGTGGAT TACTCCCTGA CTCACATTGT GGTGGACAGG 
GTTATGGCAG ATGATGGGCA ATATGAGGTC ATGTTCCTGG GAACAGATGC TGGTTCTGTT 
CTGAAGGTGG TGAGCATCAC TCAAGAGAAG TGGTCAACAG AGGAAGTGAT TCTTGAGGAG 
CTCCAGGTCT TCCAGGTTTC ATCTCCCATC TTAACATTGG AGATTTCTTC TAAAAAGCAG 
CAACTRTACG TGGGTTCCAG GGAAGGTCTC GCTCAAGTTT CCCTCAGCAG ATGTCACCTG 
TATGGCCAGG CCTGTGCTGA ATGCTGCCTA GCACGAGACC CCTACTGCGC GTGGGATGGA 
CACACATGCT CCCGATATGT CCCGGCATCT AAGAGACGTT CCAGAAGACA GGACATTAAA 
AATGGAGATC CTGCCATTCA GTGCTGGGAC ACAGAAGACA GTCACGGTGG CGGCAACGTG 
GAGGAGAGAG TTCTTTTTGG AGTTCAGAAC AACTCCACCT TCCTTGAATG CATCCCAAAG 
TCTCAACAGG CCCAAATCCG GTGGTACATG CAAAAATTGG GATCTGAACG CAGGGAAGAG 
GTCAGGCTGG ACGATCGTGT TGTCCACACA GACCGAGGAC TTCTAATTCG CTCCCTCCAT 
GCCTCTGACG TTGGCATATA CTTCTGTGTG GCTCAGGAAC ACACACACTT CACACACACT 
CTCCTTCGTC TCACGCTGCA GCTTCTCACA CACGGACAAC TTGACAAAAA ACCAAAGCTC 
AGCGAAGACG TCGTAGAGCT GCACCATAAC GCAGAGGCCC GTCAACGTTA CAAGGACTAT 
TTGAGAGTAA TGAGCTCTCC CTTCCACTCC TTRGAAGAGT ACTGCGATTC ACTTTGGCTG 
GATAAGAGGG CGTCCAGGAT GAGAGGGAAA AGTTTAGGAC ATGGCAAATG GAAACACATA 
CAGGACATGA AGAAGAATAG AAACAGGAGA CACCACAGGG ACAGAGAGGA AGACAAAGAG 
AGAAAGAGCA GAGGGAGGGT AATTAGGGCA GTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017160650.1
CDS	328..2763
Translation	MATGDSGSKRKNKAWTLFCLPVCNYGREGRRTFVSFHPLVLFVGLILLASLPFGASIKQSVPRVKLSYRELLQAGSIYLFAGPADGLHSHSLLLDEERGSLLLGARDHIYLLDPDNVGKTPRKIHWPALRDRVETCKLAGKSTNLECANFVRVLHNYNRTHVYACGTGAFHPTCAFIEISGQREDGVFQLLPNTADSGRLKCPFDPLQPFTSVLSDQYLYVGTSSDFLGKDTTFTRSLGPPPDQHYIRTDISEDYWINASPQTQRAACSLRMIIGIPQKRKQSTYSFHWNFYMNATTERGPHLQGNLHVFQNDVGGLRSLTNKWTTFLKARLVCSIPGPDGVDTHFDELQDIFLLPTRDEKNPIVYAVFSTSSSIFRGSAVCVYSMADIRAAFNGPYAHKEGPDHRWVEnullEGRIPYPRPGTCPSKTYDPRIKTTKDFPDEVISFIRFHPLMHHSVYPLAGRPVFTRVRVDYSLTHIVVDRVMADDGQYEVMFLGTDAGSVLKVVSITQEKWSTEEVILEELQVFQVSSPILTLEISSKKQQnullYVGSREGLAQVSLSRCHLYGQACAECCLARDPYCAWDGHTCSRYVPASKRRSRRQDIKNGDPAIQCWDTEDSHGGGNVEERVLFGVQNNSTFLECIPKSQQAQIRWYMQKLGSERREEVRLDDRVVHTDRGLLIRSLHASDVGIYFCVAQEHTHFTHTLLRLTLQLLTHGQLDKKPKLSEDVVELHHNAEARQRYKDYLRVMSSPFHSnullEEYCDSLWLDKRASRMRGKSLGHGKWKHIQDMKKNRNRRHHRDREEDKERKSRGRVIRAV

Target ORF information:

RefSeq Version	XM_017305161.1
Organism	Poecilia reticulata(guppy)
Definition	Poecilia reticulata semaphorin 3D (sema3d), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017305161.1

ORF Insert Sequence:

ATGGCAACAG GAGACTCTGG AAGTAAGAGG AAGAATAAAG CTTGGACTCT GTTTTGCTTG 
CCTGTCTGTA ATTATGGCAG AGAAGGACGA AGGACTTTTG TTTCGTTCCA TCCACTGGTT 
CTGTTTGTGG GGCTGATTTT GTTGGCCAGC CTTCCTTTTG GAGCGAGCAT CAAACAGAGT 
GTCCCCAGAG TCAAACTCAG CTACAGAGAA TTGCTTCAGG CTGGAAGTAT CTATCTGTTT 
GCGGGCCCTG CCGATGGCCT GCACTCCCAC TCTCTGCTGC TGGATGAGGA GAGAGGCAGT 
CTTTTGCTCG GAGCTAGGGA TCACATCTAC CTGCTTGACC CGGACAATGT GGGCAAAACC 
CCAAGGAAGA TCCACTGGCC TGCTCTGAGA GACAGAGTTG AAACATGCAA ACTTGCTGGG 
AAAAGTACAA ATTTGGAATG TGCTAACTTT GTAAGAGTCC TCCACAATTA CAACCGGACA 
CATGTCTACG CCTGTGGGAC AGGAGCCTTC CACCCCACCT GTGCTTTCAT TGAAATTTCT 
GGGCAGAGAG AGGATGGTGT TTTCCAACTG TTGCCTAATA CAGCAGATTC TGGCAGGTTG 
AAGTGTCCTT TCGATCCGTT GCAGCCGTTC ACCTCAGTCC TTTCAGATCA GTACCTGTAT 
GTGGGCACAT CATCAGACTT TCTGGGAAAA GACACTACGT TCACGCGCTC CTTAGGTCCT 
CCGCCTGACC AGCACTACAT ACGCACAGAC ATCTCTGAAG ACTACTGGAT CAACGCCTCA 
CCGCAGACAC AAAGAGCCGC CTGTAGCCTC AGGATGATTA TTGGCATTCC ACAGAAAAGG 
AAACAGTCTA CTTATTCATT TCACTGGAAC TTTTATATGA ATGCCACAAC TGAACGGGGG 
CCACACCTAC AGGGGAATTT ACATGTGTTT CAGAATGATG TCGGCGGGCT TCGGAGTCTA 
ACCAATAAGT GGACCACCTT CCTCAAAGCC AGGCTTGTGT GTTCAATTCC AGGACCAGAT 
GGTGTGGACA CACACTTTGA TGAGCTCCAG GACATCTTTC TGCTTCCTAC CAGAGATGAA 
AAAAACCCCA TAGTGTATGC AGTCTTCTCT ACTTCCAGTT CCATTTTCCG GGGCTCAGCA 
GTATGTGTAT ATAGCATGGC AGACATAAGG GCGGCGTTCA ATGGACCATA CGCCCACAAA 
GAGGGGCCCG ACCACAGATG GGTGGAATAY GAGGGGAGAA TACCATATCC CCGTCCTGGA 
ACGTGCCCCA GTAAGACGTA TGATCCAAGA ATCAAAACCA CCAAAGACTT TCCAGATGAG 
GTGATCAGTT TTATTCGATT CCACCCACTG ATGCACCACT CAGTTTACCC TCTGGCTGGT 
CGACCGGTCT TCACACGTGT CCGAGTGGAT TACTCCCTGA CTCACATTGT GGTGGACAGG 
GTTATGGCAG ATGATGGGCA ATATGAGGTC ATGTTCCTGG GAACAGATGC TGGTTCTGTT 
CTGAAGGTGG TGAGCATCAC TCAAGAGAAG TGGTCAACAG AGGAAGTGAT TCTTGAGGAG 
CTCCAGGTCT TCCAGGTTTC ATCTCCCATC TTAACATTGG AGATTTCTTC TAAAAAGCAG 
CAACTRTACG TGGGTTCCAG GGAAGGTCTC GCTCAAGTTT CCCTCAGCAG ATGTCACCTG 
TATGGCCAGG CCTGTGCTGA ATGCTGCCTA GCACGAGACC CCTACTGCGC GTGGGATGGA 
CACACATGCT CCCGATATGT CCCGGCATCT AAGAGACGTT CCAGAAGACA GGACATTAAA 
AATGGAGATC CTGCCATTCA GTGCTGGGAC ACAGAAGACA GTCACGGTGG CGGCAACGTG 
GAGGAGAGAG TTCTTTTTGG AGTTCAGAAC AACTCCACCT TCCTTGAATG CATCCCAAAG 
TCTCAACAGG CCCAAATCCG GTGGTACATG CAAAAATTGG GATCTGAACG CAGGGAAGAG 
GTCAGGCTGG ACGATCGTGT TGTCCACACA GACCGAGGAC TTCTAATTCG CTCCCTCCAT 
GCCTCTGACG TTGGCATATA CTTCTGTGTG GCTCAGGAAC ACACACACTT CACACACACT 
CTCCTTCGTC TCACGCTGCA GCTTCTCACA CACGGACAAC TTGACAAAAA ACCAAAGCTC 
AGCGAAGACG TCGTAGAGCT GCACCATAAC GCAGAGGCCC GTCAACGTTA CAAGGACTAT 
TTGAGAGTAA TGAGCTCTCC CTTCCACTCC TTRGAAGAGT ACTGCGATTC ACTTTGGCTG 
GATAAGAGGG CGTCCAGGAT GAGAGGGAAA AGTTTAGGAC ATGGCAAATG GAAACACATA 
CAGGACATGA AGAAGAATAG AAACAGGAGA CACCACAGGG ACAGAGAGGA AGACAAAGAG 
AGAAAGAGCA GAGGGAGGGT AATTAGGGCA GTGTGA