crim1 cDNA ORF clone, Poecilia latipinna(sailfin molly)

Gene
Clones
gRNAs

Summary

Gene Symbol	crim1
Entrez Gene ID	106949429
Full Name	cysteine rich transmembrane BMP regulator 1
Gene Type	protein-coding
Organism	Poecilia latipinna(sailfin molly)

mRNA and Protein(s)

mRNA	Protein	Name
XM_015035657.1	XP_014891143.1	cysteine-rich motor neuron 1 protein isoform X1
XM_015035658.1	XP_014891144.1	cysteine-rich motor neuron 1 protein isoform X2

Pathways from BioSystems
Gene Ontology

The following crim1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the crim1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OPo46330	XM_015035657.1 Latest version!	Poecilia latipinna cysteine rich transmembrane BMP regulator 1 (chordin-like) (crim1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
OPo46331	XM_015035658.1 Latest version!	Poecilia latipinna cysteine rich transmembrane BMP regulator 1 (chordin-like) (crim1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

crim1 ( XM_015035657.1 ) cDNA ORF clone, Poecilia latipinna(sailfin molly) -> XP_014891143.1 Poecilia latipinna cysteine rich transmembrane BMP regulator 1 (chordin-like) (crim1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OPo46330
Clone ID Related Accession (Same CDS sequence)	XM_015035657.1
Accession Version	XM_015035657.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2826bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-14
Organism	Poecilia latipinna(sailfin molly)
Product	cysteine-rich motor neuron 1 protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015112959.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Poecilia latipinna Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCGGCA AAAGCTCCGT TATGTTGCTG AGTCTCCTCG TTCTGGTGCG TGCGCTCCTG 
GCTCACGAGT GCACGCCATG TAACCTCCGG ACATGTCCAG TCAAAGTCGC GCAAGGCTGC 
GAGGGTGGCC GGAGCGTGGC CAGGGATCCA TGCGGGTGCT GCGACCACTG CACGCGGCTG 
GAGTGGGAGC CCTGCGGCGG CCAGGACTGG ACGCACGGCT ACTGCGCCCT GGGACTAACC 
TGCGCCTCTA TCAACAGCAC CGGAGCGGCC ACTATGCCTG AAATTGGAGT GTGTAAACTT 
CTTCCTGAAC ACCCAGATGC AGGCCTTGAG GATGAGCGCT GTCCTCTAAT AAGGGGCTGC 
GACAAAGCGG GAGCTGAATG TGTGTGTGAT GCTCGTTACT CCTGCTTGGG AACTTTTACC 
TACCCAGACC AAGAGACCTG CATGAAGGCC AGCAAGTCAG AGGGTCGAAG GCACGAGCAC 
AGGGAGAGAC ACAAAGAGAA ATATGTGGGA CCGTCTAATC CGACGTGCAT GTTCTCTGGG 
TGCAACCTGA CCGCTGACGG GTGCGTGTGT GAGTCTCAGA GCTGCCATCA CCACTTCGCC 
TACATCAACC GGAGCCAGTG CCAGGAAGCA GCAGAGGAGC TCAGGTGTGC CGGGGTGACA 
TGTCCTGCCC TGCAGGTGCC CTCTTGCCCC AAAGGGTCAG TCTTGACCAA GAGCTACACG 
CCCCCTGCAG GGTGCTGCCC CTCCATGCCG CCCCAGTGCA CATGCGATTT CCGTGCCTGC 
AACAAAACCC ACTGTCCAAG CGGCCAGCAC GCTGTTCTGA TCAGTCAGGC TAGCGGTCGG 
CCAGGAGACT GCTGTGACAT ATTTGAGTGC CAGAAAGTCT CTCCAAATTG CGTCTTCAAT 
GGCAAAAAGT TCTCAGAGGG AGAGGTGTAC CGCATGGACC CCTGCTGGCT GTGTCAGTGC 
CGCGGAGGAA TCTCCTTCTG CTCCAAAGCC GAGTGTGGTG AGCTGGACTG TGAAAAGTTC 
TACATTCCAG AGGGCGAGTG CTGCCCCGTT TGCATAGATG TTGAGTTGAT GTCGATGGAC 
AGCACTAAGG CTAGCTGCTG GGTGAATAAC AAACTCCGAA CCCACGAGGA ACAGTGGAAA 
GAAGACGACT GCACATTCTG TCAGTGTGTG GATGGGGATC CACACTGTAC AGCCATGGCC 
TGCAAACAGA GTTGCCAGAA CCCTGTAAAG ATCCCTGGAG AGTGCTGTCC CTTCTGCGAA 
GAGCCATCCT ATGAAACAGT TAGCCCTTTG CTGTGTCCAC CATTGGAAAA TTGCTCCCTT 
TCAGGGAATG AATGCCCCTA TGGCTTCCAA CAGGACCACA ATGGGTGCCT CCTCTGCCAG 
TGTCTCAACA ATGACTCCTG TCCTGACTTG GGAAAGTACT GTCCTCTGCA ATGTCCGACG 
GGATATGAGA AGGATGATTT GGGCTGCGAG GTGTGTGAGT GCAGCATCCC CACTCCCAAG 
TGCCGAACCC TGACCTGCAC AAAGACCTGC CCTTATGGAT ATGTGAGGAA CAAACATGGC 
TGTGAAATGT GTCGTTGTGT GAAATGTCCT CCCTTGACCT GCGACAAGTA CTGTAGCAAT 
GGCTATCGAC AGAACAGGAA GGGCTGCAAC ATCTGCATGT GCAAAGAAAT TGACCAAGTT 
CCAAGCACAA CCGTCCCAGC CAGCATGCCC AGCTTCTGCC TCACCTCCAA TGGCCGCAGG 
TATGAGGAGG GTGACAGCTG GCACGACGGC TGCCGTGACT GCTTCTGCCT CTCTGGGCGG 
GAGATGTGTG TCCTCATATC ATGCCCCATG CCCAGCTGCT CTCAACCTGT TGTCAAGTCT 
GACCAATGCT GTCCTACATG TGAAGATGAG TCCGGCAGTG GCCAGCCTGA GGGGATGGAC 
ATGGTGGTGT GCAGAGCACC TGGAGGGGAA TTTTATGTGG AGGGGGAAAC TTGGAACCTG 
GACGAGTGCA CGCGCTGCAC CTGCAGGAAG GGACGCGTCC TGTGCGACAC GGAAGTTTGT 
CCCCCAGCGC TTTGCCAGAC ACCCATCAGA AACAAGGACA CCTGTTGCCA TATGTGCCCA 
GAAGAGACAG AGAGCAACTT GCTGCCAGTG AACGGCAGCC AACAGGAGTT CTGCATAACC 
GCCGAAGGAG ACATGCTGCA AGCCGGGGAC TCCTGGAAGG CCAACGCCTG CACGAGCTGC 
ACGTGCAACA ACGGCACTAT TCAGTGTTTC TCCCAACACT GTCCGGCAGC CAGCTGCAGG 
GTGCCGGTTT TACTCAAGGG CCAGTGCTGC CCACATTGTC TCGAATCAAC AACATCAGCA 
TCAGTGACTG TATCCACCCG CACACCACAA GAAGTGGTCA CCACCAGCAC ATGGCAGAGT 
GAATCTTGGA TTACAACTGT GACCGTCCCA CCTGTCACCG CTGACACAGA CCAGCCACGT 
TTGGGTGAGA ACTACCCCAG CCATACAGAG ATGGCTCTTG CTTACCAATC AGCTGCATGG 
ATCCTTGCAG GACTCCTTTT GGCCATCATT ATCTTTCTCA TTGTGGCATT ACTCATCAAC 
AAGAAAAAGA AGTGGGTGCA GATGTCCTGC TACAGCGCAC CAAAGAAGAC GGTCATCCTG 
AAGAAACACG TGAACAAGAA TTCAGTCGTC TACATGGAGC CCTCCAAGGA GAATAAGTTT 
CAGAATATGA AGACTGACTG CGGCATCATC CACTTCTCTC CAGAGATGAG CTCGCCTCCC 
TGCACGGAGA AGATGACCAT TCCCAGGGCC AAACTGAGCA TGGGCAGTGA CTGGACGCCA 
CGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014891143.1
CDS	361..3186
Translation	MFGKSSVMLLSLLVLVRALLAHECTPCNLRTCPVKVAQGCEGGRSVARDPCGCCDHCTRLEWEPCGGQDWTHGYCALGLTCASINSTGAATMPEIGVCKLLPEHPDAGLEDERCPLIRGCDKAGAECVCDARYSCLGTFTYPDQETCMKASKSEGRRHEHRERHKEKYVGPSNPTCMFSGCNLTADGCVCESQSCHHHFAYINRSQCQEAAEELRCAGVTCPALQVPSCPKGSVLTKSYTPPAGCCPSMPPQCTCDFRACNKTHCPSGQHAVLISQASGRPGDCCDIFECQKVSPNCVFNGKKFSEGEVYRMDPCWLCQCRGGISFCSKAECGELDCEKFYIPEGECCPVCIDVELMSMDSTKASCWVNNKLRTHEEQWKEDDCTFCQCVDGDPHCTAMACKQSCQNPVKIPGECCPFCEEPSYETVSPLLCPPLENCSLSGNECPYGFQQDHNGCLLCQCLNNDSCPDLGKYCPLQCPTGYEKDDLGCEVCECSIPTPKCRTLTCTKTCPYGYVRNKHGCEMCRCVKCPPLTCDKYCSNGYRQNRKGCNICMCKEIDQVPSTTVPASMPSFCLTSNGRRYEEGDSWHDGCRDCFCLSGREMCVLISCPMPSCSQPVVKSDQCCPTCEDESGSGQPEGMDMVVCRAPGGEFYVEGETWNLDECTRCTCRKGRVLCDTEVCPPALCQTPIRNKDTCCHMCPEETESNLLPVNGSQQEFCITAEGDMLQAGDSWKANACTSCTCNNGTIQCFSQHCPAASCRVPVLLKGQCCPHCLESTTSASVTVSTRTPQEVVTTSTWQSESWITTVTVPPVTADTDQPRLGENYPSHTEMALAYQSAAWILAGLLLAIIIFLIVALLINKKKKWVQMSCYSAPKKTVILKKHVNKNSVVYMEPSKENKFQNMKTDCGIIHFSPEMSSPPCTEKMTIPRAKLSMGSDWTPR

Target ORF information:

RefSeq Version	XM_015035657.1
Organism	Poecilia latipinna(sailfin molly)
Definition	Poecilia latipinna cysteine rich transmembrane BMP regulator 1 (chordin-like) (crim1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015035657.1

ORF Insert Sequence:

ATGTTCGGCA AAAGCTCCGT TATGTTGCTG AGTCTCCTCG TTCTGGTGCG TGCGCTCCTG 
GCTCACGAGT GCACGCCATG TAACCTCCGG ACATGTCCAG TCAAAGTCGC GCAAGGCTGC 
GAGGGTGGCC GGAGCGTGGC CAGGGATCCA TGCGGGTGCT GCGACCACTG CACGCGGCTG 
GAGTGGGAGC CCTGCGGCGG CCAGGACTGG ACGCACGGCT ACTGCGCCCT GGGACTAACC 
TGCGCCTCTA TCAACAGCAC CGGAGCGGCC ACTATGCCTG AAATTGGAGT GTGTAAACTT 
CTTCCTGAAC ACCCAGATGC AGGCCTTGAG GATGAGCGCT GTCCTCTAAT AAGGGGCTGC 
GACAAAGCGG GAGCTGAATG TGTGTGTGAT GCTCGTTACT CCTGCTTGGG AACTTTTACC 
TACCCAGACC AAGAGACCTG CATGAAGGCC AGCAAGTCAG AGGGTCGAAG GCACGAGCAC 
AGGGAGAGAC ACAAAGAGAA ATATGTGGGA CCGTCTAATC CGACGTGCAT GTTCTCTGGG 
TGCAACCTGA CCGCTGACGG GTGCGTGTGT GAGTCTCAGA GCTGCCATCA CCACTTCGCC 
TACATCAACC GGAGCCAGTG CCAGGAAGCA GCAGAGGAGC TCAGGTGTGC CGGGGTGACA 
TGTCCTGCCC TGCAGGTGCC CTCTTGCCCC AAAGGGTCAG TCTTGACCAA GAGCTACACG 
CCCCCTGCAG GGTGCTGCCC CTCCATGCCG CCCCAGTGCA CATGCGATTT CCGTGCCTGC 
AACAAAACCC ACTGTCCAAG CGGCCAGCAC GCTGTTCTGA TCAGTCAGGC TAGCGGTCGG 
CCAGGAGACT GCTGTGACAT ATTTGAGTGC CAGAAAGTCT CTCCAAATTG CGTCTTCAAT 
GGCAAAAAGT TCTCAGAGGG AGAGGTGTAC CGCATGGACC CCTGCTGGCT GTGTCAGTGC 
CGCGGAGGAA TCTCCTTCTG CTCCAAAGCC GAGTGTGGTG AGCTGGACTG TGAAAAGTTC 
TACATTCCAG AGGGCGAGTG CTGCCCCGTT TGCATAGATG TTGAGTTGAT GTCGATGGAC 
AGCACTAAGG CTAGCTGCTG GGTGAATAAC AAACTCCGAA CCCACGAGGA ACAGTGGAAA 
GAAGACGACT GCACATTCTG TCAGTGTGTG GATGGGGATC CACACTGTAC AGCCATGGCC 
TGCAAACAGA GTTGCCAGAA CCCTGTAAAG ATCCCTGGAG AGTGCTGTCC CTTCTGCGAA 
GAGCCATCCT ATGAAACAGT TAGCCCTTTG CTGTGTCCAC CATTGGAAAA TTGCTCCCTT 
TCAGGGAATG AATGCCCCTA TGGCTTCCAA CAGGACCACA ATGGGTGCCT CCTCTGCCAG 
TGTCTCAACA ATGACTCCTG TCCTGACTTG GGAAAGTACT GTCCTCTGCA ATGTCCGACG 
GGATATGAGA AGGATGATTT GGGCTGCGAG GTGTGTGAGT GCAGCATCCC CACTCCCAAG 
TGCCGAACCC TGACCTGCAC AAAGACCTGC CCTTATGGAT ATGTGAGGAA CAAACATGGC 
TGTGAAATGT GTCGTTGTGT GAAATGTCCT CCCTTGACCT GCGACAAGTA CTGTAGCAAT 
GGCTATCGAC AGAACAGGAA GGGCTGCAAC ATCTGCATGT GCAAAGAAAT TGACCAAGTT 
CCAAGCACAA CCGTCCCAGC CAGCATGCCC AGCTTCTGCC TCACCTCCAA TGGCCGCAGG 
TATGAGGAGG GTGACAGCTG GCACGACGGC TGCCGTGACT GCTTCTGCCT CTCTGGGCGG 
GAGATGTGTG TCCTCATATC ATGCCCCATG CCCAGCTGCT CTCAACCTGT TGTCAAGTCT 
GACCAATGCT GTCCTACATG TGAAGATGAG TCCGGCAGTG GCCAGCCTGA GGGGATGGAC 
ATGGTGGTGT GCAGAGCACC TGGAGGGGAA TTTTATGTGG AGGGGGAAAC TTGGAACCTG 
GACGAGTGCA CGCGCTGCAC CTGCAGGAAG GGACGCGTCC TGTGCGACAC GGAAGTTTGT 
CCCCCAGCGC TTTGCCAGAC ACCCATCAGA AACAAGGACA CCTGTTGCCA TATGTGCCCA 
GAAGAGACAG AGAGCAACTT GCTGCCAGTG AACGGCAGCC AACAGGAGTT CTGCATAACC 
GCCGAAGGAG ACATGCTGCA AGCCGGGGAC TCCTGGAAGG CCAACGCCTG CACGAGCTGC 
ACGTGCAACA ACGGCACTAT TCAGTGTTTC TCCCAACACT GTCCGGCAGC CAGCTGCAGG 
GTGCCGGTTT TACTCAAGGG CCAGTGCTGC CCACATTGTC TCGAATCAAC AACATCAGCA 
TCAGTGACTG TATCCACCCG CACACCACAA GAAGTGGTCA CCACCAGCAC ATGGCAGAGT 
GAATCTTGGA TTACAACTGT GACCGTCCCA CCTGTCACCG CTGACACAGA CCAGCCACGT 
TTGGGTGAGA ACTACCCCAG CCATACAGAG ATGGCTCTTG CTTACCAATC AGCTGCATGG 
ATCCTTGCAG GACTCCTTTT GGCCATCATT ATCTTTCTCA TTGTGGCATT ACTCATCAAC 
AAGAAAAAGA AGTGGGTGCA GATGTCCTGC TACAGCGCAC CAAAGAAGAC GGTCATCCTG 
AAGAAACACG TGAACAAGAA TTCAGTCGTC TACATGGAGC CCTCCAAGGA GAATAAGTTT 
CAGAATATGA AGACTGACTG CGGCATCATC CACTTCTCTC CAGAGATGAG CTCGCCTCCC 
TGCACGGAGA AGATGACCAT TCCCAGGGCC AAACTGAGCA TGGGCAGTGA CTGGACGCCA 
CGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

crim1 ( XM_015035658.1 ) cDNA ORF clone, Poecilia latipinna(sailfin molly) -> XP_014891144.1 Poecilia latipinna cysteine rich transmembrane BMP regulator 1 (chordin-like) (crim1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OPo46331
Clone ID Related Accession (Same CDS sequence)	XM_015035658.1
Accession Version	XM_015035658.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2766bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-14
Organism	Poecilia latipinna(sailfin molly)
Product	cysteine-rich motor neuron 1 protein isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015112959.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Poecilia latipinna Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCGGCA AAAGCTCCGT TATGTTGCTG AGTCTCCTCG TTCTGGTGCG TGCGCTCCTG 
GCTCACGAGT GCACGCCATG TAACCTCCGG ACATGTCCAG TCAAAGTCGC GCAAGGCTGC 
GAGGGTGGCC GGAGCGTGGC CAGGGATCCA TGCGGGTGCT GCGACCACTG CACGCGGCTG 
GAGTGGGAGC CCTGCGGCGG CCAGGACTGG ACGCACGGCT ACTGCGCCCT GGGACTAACC 
TGCGCCTCTA TCAACAGCAC CGGAGCGGCC ACTATGCCTG AAATTGGAGT GTGTAAACTT 
CTTCCTGAAC ACCCAGATGC AGGCCTTGAG GATGAGCGCT GTCCTCTAAT AAGGGGCTGC 
GACAAAGCGG GAGCTGAATG TGTGTGTGAT GCTCGTTACT CCTGCTTGGG AACTTTTACC 
TACCCAGACC AAGAGACCTG CATGAAGGCC AGCAAGTCAG AGGGTCGAAG GCACGAGCAC 
AGGGAGAGAC ACAAAGAGAA ATATGTGGGA CCGTCTAATC CGACGTGCAT GTTCTCTGGG 
TGCAACCTGA CCGCTGACGG GTGCGTGTGT GAGTCTCAGA GCTGCCATCA CCACTTCGCC 
TACATCAACC GGAGCCAGTG CCAGGAAGCA GCAGAGGAGC TCAGGTGTGC CGGGGTGACA 
TGTCCTGCCC TGCAGGTGCC CTCTTGCCCC AAAGGGTCAG TCTTGACCAA GAGCTACACG 
CCCCCTGCAG GGTGCTGCCC CTCCATGCCG CCCCAGTGCA CATGCGATTT CCGTGCCTGC 
AACAAAACCC ACTGTCCAAG CGGCCAGCAC GCTGTTCTGA TCAGTCAGGC TAGCGGTCGG 
CCAGGAGACT GCTGTGACAT ATTTGAGTGC CAGAAAGTCT CTCCAAATTG CGTCTTCAAT 
GGCAAAAAGT TCTCAGAGGG AGAGGTGTAC CGCATGGACC CCTGCTGGCT GTGTCAGTGC 
CGCGGAGGAA TCTCCTTCTG CTCCAAAGCC GAGTGTGATG TTGAGTTGAT GTCGATGGAC 
AGCACTAAGG CTAGCTGCTG GGTGAATAAC AAACTCCGAA CCCACGAGGA ACAGTGGAAA 
GAAGACGACT GCACATTCTG TCAGTGTGTG GATGGGGATC CACACTGTAC AGCCATGGCC 
TGCAAACAGA GTTGCCAGAA CCCTGTAAAG ATCCCTGGAG AGTGCTGTCC CTTCTGCGAA 
GAGCCATCCT ATGAAACAGT TAGCCCTTTG CTGTGTCCAC CATTGGAAAA TTGCTCCCTT 
TCAGGGAATG AATGCCCCTA TGGCTTCCAA CAGGACCACA ATGGGTGCCT CCTCTGCCAG 
TGTCTCAACA ATGACTCCTG TCCTGACTTG GGAAAGTACT GTCCTCTGCA ATGTCCGACG 
GGATATGAGA AGGATGATTT GGGCTGCGAG GTGTGTGAGT GCAGCATCCC CACTCCCAAG 
TGCCGAACCC TGACCTGCAC AAAGACCTGC CCTTATGGAT ATGTGAGGAA CAAACATGGC 
TGTGAAATGT GTCGTTGTGT GAAATGTCCT CCCTTGACCT GCGACAAGTA CTGTAGCAAT 
GGCTATCGAC AGAACAGGAA GGGCTGCAAC ATCTGCATGT GCAAAGAAAT TGACCAAGTT 
CCAAGCACAA CCGTCCCAGC CAGCATGCCC AGCTTCTGCC TCACCTCCAA TGGCCGCAGG 
TATGAGGAGG GTGACAGCTG GCACGACGGC TGCCGTGACT GCTTCTGCCT CTCTGGGCGG 
GAGATGTGTG TCCTCATATC ATGCCCCATG CCCAGCTGCT CTCAACCTGT TGTCAAGTCT 
GACCAATGCT GTCCTACATG TGAAGATGAG TCCGGCAGTG GCCAGCCTGA GGGGATGGAC 
ATGGTGGTGT GCAGAGCACC TGGAGGGGAA TTTTATGTGG AGGGGGAAAC TTGGAACCTG 
GACGAGTGCA CGCGCTGCAC CTGCAGGAAG GGACGCGTCC TGTGCGACAC GGAAGTTTGT 
CCCCCAGCGC TTTGCCAGAC ACCCATCAGA AACAAGGACA CCTGTTGCCA TATGTGCCCA 
GAAGAGACAG AGAGCAACTT GCTGCCAGTG AACGGCAGCC AACAGGAGTT CTGCATAACC 
GCCGAAGGAG ACATGCTGCA AGCCGGGGAC TCCTGGAAGG CCAACGCCTG CACGAGCTGC 
ACGTGCAACA ACGGCACTAT TCAGTGTTTC TCCCAACACT GTCCGGCAGC CAGCTGCAGG 
GTGCCGGTTT TACTCAAGGG CCAGTGCTGC CCACATTGTC TCGAATCAAC AACATCAGCA 
TCAGTGACTG TATCCACCCG CACACCACAA GAAGTGGTCA CCACCAGCAC ATGGCAGAGT 
GAATCTTGGA TTACAACTGT GACCGTCCCA CCTGTCACCG CTGACACAGA CCAGCCACGT 
TTGGGTGAGA ACTACCCCAG CCATACAGAG ATGGCTCTTG CTTACCAATC AGCTGCATGG 
ATCCTTGCAG GACTCCTTTT GGCCATCATT ATCTTTCTCA TTGTGGCATT ACTCATCAAC 
AAGAAAAAGA AGTGGGTGCA GATGTCCTGC TACAGCGCAC CAAAGAAGAC GGTCATCCTG 
AAGAAACACG TGAACAAGAA TTCAGTCGTC TACATGGAGC CCTCCAAGGA GAATAAGTTT 
CAGAATATGA AGACTGACTG CGGCATCATC CACTTCTCTC CAGAGATGAG CTCGCCTCCC 
TGCACGGAGA AGATGACCAT TCCCAGGGCC AAACTGAGCA TGGGCAGTGA CTGGACGCCA 
CGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014891144.1
CDS	362..3127
Translation	MFGKSSVMLLSLLVLVRALLAHECTPCNLRTCPVKVAQGCEGGRSVARDPCGCCDHCTRLEWEPCGGQDWTHGYCALGLTCASINSTGAATMPEIGVCKLLPEHPDAGLEDERCPLIRGCDKAGAECVCDARYSCLGTFTYPDQETCMKASKSEGRRHEHRERHKEKYVGPSNPTCMFSGCNLTADGCVCESQSCHHHFAYINRSQCQEAAEELRCAGVTCPALQVPSCPKGSVLTKSYTPPAGCCPSMPPQCTCDFRACNKTHCPSGQHAVLISQASGRPGDCCDIFECQKVSPNCVFNGKKFSEGEVYRMDPCWLCQCRGGISFCSKAECDVELMSMDSTKASCWVNNKLRTHEEQWKEDDCTFCQCVDGDPHCTAMACKQSCQNPVKIPGECCPFCEEPSYETVSPLLCPPLENCSLSGNECPYGFQQDHNGCLLCQCLNNDSCPDLGKYCPLQCPTGYEKDDLGCEVCECSIPTPKCRTLTCTKTCPYGYVRNKHGCEMCRCVKCPPLTCDKYCSNGYRQNRKGCNICMCKEIDQVPSTTVPASMPSFCLTSNGRRYEEGDSWHDGCRDCFCLSGREMCVLISCPMPSCSQPVVKSDQCCPTCEDESGSGQPEGMDMVVCRAPGGEFYVEGETWNLDECTRCTCRKGRVLCDTEVCPPALCQTPIRNKDTCCHMCPEETESNLLPVNGSQQEFCITAEGDMLQAGDSWKANACTSCTCNNGTIQCFSQHCPAASCRVPVLLKGQCCPHCLESTTSASVTVSTRTPQEVVTTSTWQSESWITTVTVPPVTADTDQPRLGENYPSHTEMALAYQSAAWILAGLLLAIIIFLIVALLINKKKKWVQMSCYSAPKKTVILKKHVNKNSVVYMEPSKENKFQNMKTDCGIIHFSPEMSSPPCTEKMTIPRAKLSMGSDWTPR

Target ORF information:

RefSeq Version	XM_015035658.1
Organism	Poecilia latipinna(sailfin molly)
Definition	Poecilia latipinna cysteine rich transmembrane BMP regulator 1 (chordin-like) (crim1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015035658.1

ORF Insert Sequence:

ATGTTCGGCA AAAGCTCCGT TATGTTGCTG AGTCTCCTCG TTCTGGTGCG TGCGCTCCTG 
GCTCACGAGT GCACGCCATG TAACCTCCGG ACATGTCCAG TCAAAGTCGC GCAAGGCTGC 
GAGGGTGGCC GGAGCGTGGC CAGGGATCCA TGCGGGTGCT GCGACCACTG CACGCGGCTG 
GAGTGGGAGC CCTGCGGCGG CCAGGACTGG ACGCACGGCT ACTGCGCCCT GGGACTAACC 
TGCGCCTCTA TCAACAGCAC CGGAGCGGCC ACTATGCCTG AAATTGGAGT GTGTAAACTT 
CTTCCTGAAC ACCCAGATGC AGGCCTTGAG GATGAGCGCT GTCCTCTAAT AAGGGGCTGC 
GACAAAGCGG GAGCTGAATG TGTGTGTGAT GCTCGTTACT CCTGCTTGGG AACTTTTACC 
TACCCAGACC AAGAGACCTG CATGAAGGCC AGCAAGTCAG AGGGTCGAAG GCACGAGCAC 
AGGGAGAGAC ACAAAGAGAA ATATGTGGGA CCGTCTAATC CGACGTGCAT GTTCTCTGGG 
TGCAACCTGA CCGCTGACGG GTGCGTGTGT GAGTCTCAGA GCTGCCATCA CCACTTCGCC 
TACATCAACC GGAGCCAGTG CCAGGAAGCA GCAGAGGAGC TCAGGTGTGC CGGGGTGACA 
TGTCCTGCCC TGCAGGTGCC CTCTTGCCCC AAAGGGTCAG TCTTGACCAA GAGCTACACG 
CCCCCTGCAG GGTGCTGCCC CTCCATGCCG CCCCAGTGCA CATGCGATTT CCGTGCCTGC 
AACAAAACCC ACTGTCCAAG CGGCCAGCAC GCTGTTCTGA TCAGTCAGGC TAGCGGTCGG 
CCAGGAGACT GCTGTGACAT ATTTGAGTGC CAGAAAGTCT CTCCAAATTG CGTCTTCAAT 
GGCAAAAAGT TCTCAGAGGG AGAGGTGTAC CGCATGGACC CCTGCTGGCT GTGTCAGTGC 
CGCGGAGGAA TCTCCTTCTG CTCCAAAGCC GAGTGTGATG TTGAGTTGAT GTCGATGGAC 
AGCACTAAGG CTAGCTGCTG GGTGAATAAC AAACTCCGAA CCCACGAGGA ACAGTGGAAA 
GAAGACGACT GCACATTCTG TCAGTGTGTG GATGGGGATC CACACTGTAC AGCCATGGCC 
TGCAAACAGA GTTGCCAGAA CCCTGTAAAG ATCCCTGGAG AGTGCTGTCC CTTCTGCGAA 
GAGCCATCCT ATGAAACAGT TAGCCCTTTG CTGTGTCCAC CATTGGAAAA TTGCTCCCTT 
TCAGGGAATG AATGCCCCTA TGGCTTCCAA CAGGACCACA ATGGGTGCCT CCTCTGCCAG 
TGTCTCAACA ATGACTCCTG TCCTGACTTG GGAAAGTACT GTCCTCTGCA ATGTCCGACG 
GGATATGAGA AGGATGATTT GGGCTGCGAG GTGTGTGAGT GCAGCATCCC CACTCCCAAG 
TGCCGAACCC TGACCTGCAC AAAGACCTGC CCTTATGGAT ATGTGAGGAA CAAACATGGC 
TGTGAAATGT GTCGTTGTGT GAAATGTCCT CCCTTGACCT GCGACAAGTA CTGTAGCAAT 
GGCTATCGAC AGAACAGGAA GGGCTGCAAC ATCTGCATGT GCAAAGAAAT TGACCAAGTT 
CCAAGCACAA CCGTCCCAGC CAGCATGCCC AGCTTCTGCC TCACCTCCAA TGGCCGCAGG 
TATGAGGAGG GTGACAGCTG GCACGACGGC TGCCGTGACT GCTTCTGCCT CTCTGGGCGG 
GAGATGTGTG TCCTCATATC ATGCCCCATG CCCAGCTGCT CTCAACCTGT TGTCAAGTCT 
GACCAATGCT GTCCTACATG TGAAGATGAG TCCGGCAGTG GCCAGCCTGA GGGGATGGAC 
ATGGTGGTGT GCAGAGCACC TGGAGGGGAA TTTTATGTGG AGGGGGAAAC TTGGAACCTG 
GACGAGTGCA CGCGCTGCAC CTGCAGGAAG GGACGCGTCC TGTGCGACAC GGAAGTTTGT 
CCCCCAGCGC TTTGCCAGAC ACCCATCAGA AACAAGGACA CCTGTTGCCA TATGTGCCCA 
GAAGAGACAG AGAGCAACTT GCTGCCAGTG AACGGCAGCC AACAGGAGTT CTGCATAACC 
GCCGAAGGAG ACATGCTGCA AGCCGGGGAC TCCTGGAAGG CCAACGCCTG CACGAGCTGC 
ACGTGCAACA ACGGCACTAT TCAGTGTTTC TCCCAACACT GTCCGGCAGC CAGCTGCAGG 
GTGCCGGTTT TACTCAAGGG CCAGTGCTGC CCACATTGTC TCGAATCAAC AACATCAGCA 
TCAGTGACTG TATCCACCCG CACACCACAA GAAGTGGTCA CCACCAGCAC ATGGCAGAGT 
GAATCTTGGA TTACAACTGT GACCGTCCCA CCTGTCACCG CTGACACAGA CCAGCCACGT 
TTGGGTGAGA ACTACCCCAG CCATACAGAG ATGGCTCTTG CTTACCAATC AGCTGCATGG 
ATCCTTGCAG GACTCCTTTT GGCCATCATT ATCTTTCTCA TTGTGGCATT ACTCATCAAC 
AAGAAAAAGA AGTGGGTGCA GATGTCCTGC TACAGCGCAC CAAAGAAGAC GGTCATCCTG 
AAGAAACACG TGAACAAGAA TTCAGTCGTC TACATGGAGC CCTCCAAGGA GAATAAGTTT 
CAGAATATGA AGACTGACTG CGGCATCATC CACTTCTCTC CAGAGATGAG CTCGCCTCCC 
TGCACGGAGA AGATGACCAT TCCCAGGGCC AAACTGAGCA TGGGCAGTGA CTGGACGCCA 
CGCTAA