gtf2e1 cDNA ORF clone, Poecilia formosa(Amazon molly)

Gene
Clones
gRNAs

Summary

Gene Symbol	gtf2e1
Entrez Gene ID	103150638
Full Name	general transcription factor IIE subunit 1
Gene Type	protein-coding
Organism	Poecilia formosa(Amazon molly)

mRNA and Protein(s)

mRNA	Protein	Name
XM_007570338.2	XP_007570400.1	general transcription factor IIE subunit 1

Pathways from BioSystems
Gene Ontology

The following gtf2e1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the gtf2e1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OPo152487	XM_007570338.2 Latest version!	Poecilia formosa general transcription factor IIE subunit 1 (gtf2e1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$258.30 $369.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

gtf2e1 ( XM_007570338.2 ) cDNA ORF clone, Poecilia formosa(Amazon molly) -> XP_007570400.1 Poecilia formosa general transcription factor IIE subunit 1 (gtf2e1), mRNA.

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CloneID	OPo152487
Clone ID Related Accession (Same CDS sequence)	XM_007570338.2
Accession Version	XM_007570338.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1344bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-05-02
Organism	Poecilia formosa(Amazon molly)
Product	general transcription factor IIE subunit 1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_006800198.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On May 3, 2016 this sequence version replaced XM_007570338.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Poecilia formosa Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATTGAAC CTGAGGTACT AACCGAAGTC CCTGCGTCTC TGAAGCGGCT GGCTAAGCAA 
GTTGTAAGGG GTTTTTATGG AGTAGAACAT GCCTTGGCTC TGGACGTGCT GATACGTAAC 
CCATGTGTCC GAGAAGAGGA CATGCTGGAG CTGCTGAAGT TTGACCGGAA ACAGCTGCGC 
TCTGTGCTCA ACACCCTGAA GGCGGACAAG TTTGTCAAGT GCCGTATGAG AGTGGAGACA 
GCCCCTGATG GGAAGACGAC ACGTCACAAC TACTATTTCA TTAACTACAG AGTGCTGGTT 
AACGTTGTAA AGTACAAATT GGACCACATG CGACGTCGCA TTGAGACAGA CGAACGGGAC 
TCCACCAGCC GCGCCTCCTT CAGATGCCCC TGCTGCTTCT CCACTTTCAC TGACCTGGAG 
GCCAATCAGC TTTTTGATCA CATGACTGGT ACGTTTCGCT GCACCTTTTG TCAGACTGAG 
GTAGAAGAAG ATGAATCGGT CTGCCCTGAT GCCAGGACAC TTGTGGCCCG CTTTAATGAG 
CAAATCGAGC CCATCTACGC ACTCCTACGC GAAACCGAAG ATATTAACTT GTCCCATGAC 
CTGCTGGAGC CGGAGCCCAC CGAGATCCCC GCTCTCAAGC AGAGCCGTGA ACGAGCTGCA 
GCTGCTGCAG GAAATGCTGC AGGCGGACCA CACCGCGAAG CCTGGTCTAA TAAAGGATCG 
ACCTACGCTG ATTTGTACAT CCAGAATGTG GTTATCAGCA TGGAGGAGCA GGATGACCAA 
AAGAGGCAGG TCAATGAGGG CAAGCCCCCC AAGGAGCGGC CCGTTTGGCT GACGCAGAGC 
ACGGTGCAGG GGGCTTACAA CGAGCCGGAT GCTCTCAAGA GCCGCGGCGA CATCGCAGCC 
GGAGCTCAGG ACGGCGCAAC CGGTCACGGG GCAGGCCAGG CGGACGAAAA CGAGGAAGTG 
ATGCGCGCCC TGCTCATCCA CGAGAAGAGA GCCGTCGCGG GAGCAGGTGT GGGTGGAGCG 
AGCGCAGCTG CCAGGGGTCT TGCCTCCGCC ACAGCTAACG CCAGTGACTC AGAGAGCGAC 
ACAAGTGAAT CAGAGGACGA ACTCCCCTCG GGCCCCACCA CTGCCACAGC CTCTCAGCAT 
CGCACCGTTG AAGAGGTCGA AGAAGAGGAC GACGACGACG ACTTCGAGGA GGTAGGGGAT 
GAGCCGATGG TGATGGTGGG TGGTCGGCAG TTCTCATACA GAGAGGTGGG CGAGAGGCCG 
GAGCTGGTGG AGCAGATGTC TATGCAGGAA AAGGAGGCTT ACATTGAGAT GGGACAAAAC 
CTCTTCCAGG ATATGTTCTA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_007570400.1
CDS	201..1544
Translation	MIEPEVLTEVPASLKRLAKQVVRGFYGVEHALALDVLIRNPCVREEDMLELLKFDRKQLRSVLNTLKADKFVKCRMRVETAPDGKTTRHNYYFINYRVLVNVVKYKLDHMRRRIETDERDSTSRASFRCPCCFSTFTDLEANQLFDHMTGTFRCTFCQTEVEEDESVCPDARTLVARFNEQIEPIYALLRETEDINLSHDLLEPEPTEIPALKQSRERAAAAAGNAAGGPHREAWSNKGSTYADLYIQNVVISMEEQDDQKRQVNEGKPPKERPVWLTQSTVQGAYNEPDALKSRGDIAAGAQDGATGHGAGQADENEEVMRALLIHEKRAVAGAGVGGASAAARGLASATANASDSESDTSESEDELPSGPTTATASQHRTVEEVEEEDDDDDFEEVGDEPMVMVGGRQFSYREVGERPELVEQMSMQEKEAYIEMGQNLFQDMFY

Target ORF information:

RefSeq Version	XM_007570338.2
Organism	Poecilia formosa(Amazon molly)
Definition	Poecilia formosa general transcription factor IIE subunit 1 (gtf2e1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_007570338.2

ORF Insert Sequence:

ATGATTGAAC CTGAGGTACT AACCGAAGTC CCTGCGTCTC TGAAGCGGCT GGCTAAGCAA 
GTTGTAAGGG GTTTTTATGG AGTAGAACAT GCCTTGGCTC TGGACGTGCT GATACGTAAC 
CCATGTGTCC GAGAAGAGGA CATGCTGGAG CTGCTGAAGT TTGACCGGAA ACAGCTGCGC 
TCTGTGCTCA ACACCCTGAA GGCGGACAAG TTTGTCAAGT GCCGTATGAG AGTGGAGACA 
GCCCCTGATG GGAAGACGAC ACGTCACAAC TACTATTTCA TTAACTACAG AGTGCTGGTT 
AACGTTGTAA AGTACAAATT GGACCACATG CGACGTCGCA TTGAGACAGA CGAACGGGAC 
TCCACCAGCC GCGCCTCCTT CAGATGCCCC TGCTGCTTCT CCACTTTCAC TGACCTGGAG 
GCCAATCAGC TTTTTGATCA CATGACTGGT ACGTTTCGCT GCACCTTTTG TCAGACTGAG 
GTAGAAGAAG ATGAATCGGT CTGCCCTGAT GCCAGGACAC TTGTGGCCCG CTTTAATGAG 
CAAATCGAGC CCATCTACGC ACTCCTACGC GAAACCGAAG ATATTAACTT GTCCCATGAC 
CTGCTGGAGC CGGAGCCCAC CGAGATCCCC GCTCTCAAGC AGAGCCGTGA ACGAGCTGCA 
GCTGCTGCAG GAAATGCTGC AGGCGGACCA CACCGCGAAG CCTGGTCTAA TAAAGGATCG 
ACCTACGCTG ATTTGTACAT CCAGAATGTG GTTATCAGCA TGGAGGAGCA GGATGACCAA 
AAGAGGCAGG TCAATGAGGG CAAGCCCCCC AAGGAGCGGC CCGTTTGGCT GACGCAGAGC 
ACGGTGCAGG GGGCTTACAA CGAGCCGGAT GCTCTCAAGA GCCGCGGCGA CATCGCAGCC 
GGAGCTCAGG ACGGCGCAAC CGGTCACGGG GCAGGCCAGG CGGACGAAAA CGAGGAAGTG 
ATGCGCGCCC TGCTCATCCA CGAGAAGAGA GCCGTCGCGG GAGCAGGTGT GGGTGGAGCG 
AGCGCAGCTG CCAGGGGTCT TGCCTCCGCC ACAGCTAACG CCAGTGACTC AGAGAGCGAC 
ACAAGTGAAT CAGAGGACGA ACTCCCCTCG GGCCCCACCA CTGCCACAGC CTCTCAGCAT 
CGCACCGTTG AAGAGGTCGA AGAAGAGGAC GACGACGACG ACTTCGAGGA GGTAGGGGAT 
GAGCCGATGG TGATGGTGGG TGGTCGGCAG TTCTCATACA GAGAGGTGGG CGAGAGGCCG 
GAGCTGGTGG AGCAGATGTC TATGCAGGAA AAGGAGGCTT ACATTGAGAT GGGACAAAAC 
CTCTTCCAGG ATATGTTCTA CTGA