cfap58 cDNA ORF clone, Poecilia formosa(Amazon molly)

Gene
Clones
gRNAs

Summary

Gene Symbol	cfap58
Entrez Gene ID	103141086
Full Name	cilia and flagella associated protein 58
Synonyms	ccdc147
Gene Type	protein-coding
Organism	Poecilia formosa(Amazon molly)

mRNA and Protein(s)

mRNA	Protein	Name
XM_007556527.2	XP_007556589.1	cilia- and flagella-associated protein 58 isoform X1
XM_016673562.1	XP_016529048.1	cilia- and flagella-associated protein 58 isoform X2

Pathways from BioSystems
Gene Ontology

The following cfap58 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the cfap58 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OPo140006	XM_007556527.2 Latest version!	Poecilia formosa cilia and flagella associated protein 58 (cfap58), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
OPo140007	XM_016673562.1 Latest version!	Poecilia formosa cilia and flagella associated protein 58 (cfap58), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

cfap58 ( XM_007556527.2 ) cDNA ORF clone, Poecilia formosa(Amazon molly) -> XP_007556589.1 Poecilia formosa cilia and flagella associated protein 58 (cfap58), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OPo140006
Clone ID Related Accession (Same CDS sequence)	XM_007556527.2
Accession Version	XM_007556527.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2589bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-05-02
Organism	Poecilia formosa(Amazon molly)
Product	cilia- and flagella-associated protein 58 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_006800018.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On May 3, 2016 this sequence version replaced XM_007556527.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Poecilia formosa Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGAAA CAGAATTTGA AGGAGACGAC CGTTTTGAGT CGTTGGAGGA GTTTCAGGTT 
GTCCTCAGTG AGCTGTCCAG TGACAGCAGC CTGGACAGGA TCAGGGCGGA GTACGAGAAA 
CTCGTCCATG CTCTGAAGAA GTCGCGGGAG AATGAAAAGA GGCTGATGTC GAAATGCAGG 
GAGCTGAACG CAGAGATCGC GTCGACATCA ACCAAAGTGG CAACTGCCCT GAAACTGTCC 
CAGGAAGATG AGATGACAAT AACGTCGCTC AAGAGGGAGT TGGATAAGGT TTGGAAGTTG 
ATTGATGCAG CTCATGATAA AGAGAGAAAG GACCAGGAAA CAATCAAGGA TTTAAAAGAA 
GACGTTTTCA AGCTCACAAA GGCAACTGAG CAAAAAACCG GACATCAGCA GGACGAAGAA 
CGAATGGATT TAATTAAAAT GATTGAAGAG TTGACTAAGG AGAGAGACCA GCTGACAAAT 
GCGGCGGCGG ACCTAAGAGA GAAACTAAAC AAGGCGACTG TAACTCAGCA AGACATAGAG 
GCTCAAAAAG AGACAGCCGT AGAGAAGATC TTGAAGCTTC AGCAGGAGCT CCAGGTGCAA 
CAGAACGAGC TCTCCAGAGA GACGAGACTG AAGGAGAAGC TCGACAAGGA GATCCAGCAG 
CTGCACACCG ACATGGAGGC CAAGATGGCC GAAATCAAAG CTCTGACCAC GCAGGGACAG 
AAGGCCAGAG AGGAGCAGCA GCGACTGGAG CAGCAGCTCA GAGATCTAAA GGTATTGCAT 
GAGCGCTTGA CCAAAGAACT GGAGCAGATT CAGGCGAAGA GCAGCAAACT GCAGCAGGAA 
TGTGAACGTC TCTTGGCCGC CAGGGAACAG CTCTCTGTGG AGAATCATCT GAAGGCAAAC 
GAACTGAAGA TAAGAGAGGA GGAGCTGAGT CACATGCGTC AGGAGGTCGC CAAACAGACC 
AAGATGAGAG AAGCCATCCA GAAGAAGTAC CACCACATGG AGGACCAGAA AGCTGAACTC 
GACCGACAGA GGGAGACGCT GAAAGCTCAG ATTGCTGGCC TGGAAAAAGA TCTTGAATCT 
TCCCGGAAGC AAGTGGAAGT CGATAAAAAA GCCAAGGAGG AGCTGATCCG AGAGAGGGCC 
AATCTGCACA AGAACATGAC CAAAGCCGTG CAGTCGGCTG AGAAGCAGCA GGACCTCATG 
AAGCTCCTGG AGCAGGACAA GAGAACCTTG GAGCACGAGA TCTCCGGGTA CCGGCAGGAG 
GCGCAGAAGC AGCGGAAGAT CATCCAGCAG CTGGAGAAGG AGCGGGACCG CTACGTCAAC 
GAGAGCGGCA GCCTTATGCA GAAGGTGCAA CAGAAGCTCA CTGTTGTCGA AGACAAAGAG 
ATGGAGATAT TTGATTGGAA AAAGAAAGCT ACAGAGTCGG AGTGTAAACT CAAACAGCAG 
GAGAACCTGC TGGAGTCGAT CATAGCGGAG AGGAATCTAT ACAGCAAAAA CCTCATCGAG 
TCTCAGGAGG AGATTTCTGA AATGAAGAGG AAAATAAAGA CTATGAGCAA CCAAGTTGCC 
CGGCTTAAAG ACGACATCTC TGAGAAGGAG CAGGAACTGG TCAGAGATCA GCAGGAGCAG 
AAGAGGCTGG AGAAGGAGAA CGAAGCCTAC AAGGGGGATC TACAGGCAAT AAAACTGCAG 
CTGGAGGAAA CGAAGCAGCT TATTGACAGC CAGAGGACAG AACAGCAAAA ACTGCAGATG 
ACAATCACCA GCATGGAGGA AGACCGAGTC CAGGAGCAGA AGCAGCTGCA GCAGGCGATC 
CGAGAGCGGG ACAGCCTCGG CAAGCAGCTG CTGCAGCGGA GTGATGAGCG CGCTCTCCTG 
CTTGAGAAGA TCCGGATCCA GCAGTCCATC CTCAGCAAGG GGGACTTCCA CTACAAGCAG 
AGGCTGGAGG ACATCCAGCT GCTGAAGCTG GAGATCAAGA GGCTGCGCCG CAGCAACGAC 
ATCCAGGACA GGACCTTACC CAACACAGAG GAGCTGAGGA CGGAGCAGCT CCGCCTGCAG 
ACGGAGCTGC TCAGAGAGAG AACCAGGAAC AGTGTTCTGG AGGACCAGCT CAAGCCCATA 
AATATTCACC GATGGAGGCA ACTGGAGGGC AGCGACCCGG GAAAGTATCA GCTCATCCAG 
AAGATTCAGG CCCTGCAGAA GCGACTGATT GCTAAAACCC AGGAGCTGGA GGAACGGGGA 
CTGCTGCTGC AGGAAAAGGA GAAGCTGTAT GTGGAACTGA AGCAGGTTCT GGCCCGACGG 
CCCGGTCCCG AGGCAGCCAT GCAGCTCCAG CAGTACCGCT GGACCATCGG GGACCGGACC 
AAAAAGCTGC AGGCTCTGAT GGCAGAGCTG AGGACGGTCG ACTCAAAGAT GAACGAATAC 
AAACGGGAGA ATCAAAGACT GAGCGGCGAG CTGGCAAATA TGAAGAAGAA GTATTTGAGT 
CAGAAAAGGC GTCATAGTGA GCAGAGAAGC AGGACCACGG CGCAGGAACA GGAAGCTCTG 
CCCCAGCTGA GCAGCACGCC TCGCTTCGCC AGAGCGGCCT TCAGGGTTTA CAACCCGGTC 
AAACTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_007556589.1
CDS	96..2684
Translation	MEETEFEGDDRFESLEEFQVVLSELSSDSSLDRIRAEYEKLVHALKKSRENEKRLMSKCRELNAEIASTSTKVATALKLSQEDEMTITSLKRELDKVWKLIDAAHDKERKDQETIKDLKEDVFKLTKATEQKTGHQQDEERMDLIKMIEELTKERDQLTNAAADLREKLNKATVTQQDIEAQKETAVEKILKLQQELQVQQNELSRETRLKEKLDKEIQQLHTDMEAKMAEIKALTTQGQKAREEQQRLEQQLRDLKVLHERLTKELEQIQAKSSKLQQECERLLAAREQLSVENHLKANELKIREEELSHMRQEVAKQTKMREAIQKKYHHMEDQKAELDRQRETLKAQIAGLEKDLESSRKQVEVDKKAKEELIRERANLHKNMTKAVQSAEKQQDLMKLLEQDKRTLEHEISGYRQEAQKQRKIIQQLEKERDRYVNESGSLMQKVQQKLTVVEDKEMEIFDWKKKATESECKLKQQENLLESIIAERNLYSKNLIESQEEISEMKRKIKTMSNQVARLKDDISEKEQELVRDQQEQKRLEKENEAYKGDLQAIKLQLEETKQLIDSQRTEQQKLQMTITSMEEDRVQEQKQLQQAIRERDSLGKQLLQRSDERALLLEKIRIQQSILSKGDFHYKQRLEDIQLLKLEIKRLRRSNDIQDRTLPNTEELRTEQLRLQTELLRERTRNSVLEDQLKPINIHRWRQLEGSDPGKYQLIQKIQALQKRLIAKTQELEERGLLLQEKEKLYVELKQVLARRPGPEAAMQLQQYRWTIGDRTKKLQALMAELRTVDSKMNEYKRENQRLSGELANMKKKYLSQKRRHSEQRSRTTAQEQEALPQLSSTPRFARAAFRVYNPVKL

Target ORF information:

RefSeq Version	XM_007556527.2
Organism	Poecilia formosa(Amazon molly)
Definition	Poecilia formosa cilia and flagella associated protein 58 (cfap58), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_007556527.2

ORF Insert Sequence:

ATGGAGGAAA CAGAATTTGA AGGAGACGAC CGTTTTGAGT CGTTGGAGGA GTTTCAGGTT 
GTCCTCAGTG AGCTGTCCAG TGACAGCAGC CTGGACAGGA TCAGGGCGGA GTACGAGAAA 
CTCGTCCATG CTCTGAAGAA GTCGCGGGAG AATGAAAAGA GGCTGATGTC GAAATGCAGG 
GAGCTGAACG CAGAGATCGC GTCGACATCA ACCAAAGTGG CAACTGCCCT GAAACTGTCC 
CAGGAAGATG AGATGACAAT AACGTCGCTC AAGAGGGAGT TGGATAAGGT TTGGAAGTTG 
ATTGATGCAG CTCATGATAA AGAGAGAAAG GACCAGGAAA CAATCAAGGA TTTAAAAGAA 
GACGTTTTCA AGCTCACAAA GGCAACTGAG CAAAAAACCG GACATCAGCA GGACGAAGAA 
CGAATGGATT TAATTAAAAT GATTGAAGAG TTGACTAAGG AGAGAGACCA GCTGACAAAT 
GCGGCGGCGG ACCTAAGAGA GAAACTAAAC AAGGCGACTG TAACTCAGCA AGACATAGAG 
GCTCAAAAAG AGACAGCCGT AGAGAAGATC TTGAAGCTTC AGCAGGAGCT CCAGGTGCAA 
CAGAACGAGC TCTCCAGAGA GACGAGACTG AAGGAGAAGC TCGACAAGGA GATCCAGCAG 
CTGCACACCG ACATGGAGGC CAAGATGGCC GAAATCAAAG CTCTGACCAC GCAGGGACAG 
AAGGCCAGAG AGGAGCAGCA GCGACTGGAG CAGCAGCTCA GAGATCTAAA GGTATTGCAT 
GAGCGCTTGA CCAAAGAACT GGAGCAGATT CAGGCGAAGA GCAGCAAACT GCAGCAGGAA 
TGTGAACGTC TCTTGGCCGC CAGGGAACAG CTCTCTGTGG AGAATCATCT GAAGGCAAAC 
GAACTGAAGA TAAGAGAGGA GGAGCTGAGT CACATGCGTC AGGAGGTCGC CAAACAGACC 
AAGATGAGAG AAGCCATCCA GAAGAAGTAC CACCACATGG AGGACCAGAA AGCTGAACTC 
GACCGACAGA GGGAGACGCT GAAAGCTCAG ATTGCTGGCC TGGAAAAAGA TCTTGAATCT 
TCCCGGAAGC AAGTGGAAGT CGATAAAAAA GCCAAGGAGG AGCTGATCCG AGAGAGGGCC 
AATCTGCACA AGAACATGAC CAAAGCCGTG CAGTCGGCTG AGAAGCAGCA GGACCTCATG 
AAGCTCCTGG AGCAGGACAA GAGAACCTTG GAGCACGAGA TCTCCGGGTA CCGGCAGGAG 
GCGCAGAAGC AGCGGAAGAT CATCCAGCAG CTGGAGAAGG AGCGGGACCG CTACGTCAAC 
GAGAGCGGCA GCCTTATGCA GAAGGTGCAA CAGAAGCTCA CTGTTGTCGA AGACAAAGAG 
ATGGAGATAT TTGATTGGAA AAAGAAAGCT ACAGAGTCGG AGTGTAAACT CAAACAGCAG 
GAGAACCTGC TGGAGTCGAT CATAGCGGAG AGGAATCTAT ACAGCAAAAA CCTCATCGAG 
TCTCAGGAGG AGATTTCTGA AATGAAGAGG AAAATAAAGA CTATGAGCAA CCAAGTTGCC 
CGGCTTAAAG ACGACATCTC TGAGAAGGAG CAGGAACTGG TCAGAGATCA GCAGGAGCAG 
AAGAGGCTGG AGAAGGAGAA CGAAGCCTAC AAGGGGGATC TACAGGCAAT AAAACTGCAG 
CTGGAGGAAA CGAAGCAGCT TATTGACAGC CAGAGGACAG AACAGCAAAA ACTGCAGATG 
ACAATCACCA GCATGGAGGA AGACCGAGTC CAGGAGCAGA AGCAGCTGCA GCAGGCGATC 
CGAGAGCGGG ACAGCCTCGG CAAGCAGCTG CTGCAGCGGA GTGATGAGCG CGCTCTCCTG 
CTTGAGAAGA TCCGGATCCA GCAGTCCATC CTCAGCAAGG GGGACTTCCA CTACAAGCAG 
AGGCTGGAGG ACATCCAGCT GCTGAAGCTG GAGATCAAGA GGCTGCGCCG CAGCAACGAC 
ATCCAGGACA GGACCTTACC CAACACAGAG GAGCTGAGGA CGGAGCAGCT CCGCCTGCAG 
ACGGAGCTGC TCAGAGAGAG AACCAGGAAC AGTGTTCTGG AGGACCAGCT CAAGCCCATA 
AATATTCACC GATGGAGGCA ACTGGAGGGC AGCGACCCGG GAAAGTATCA GCTCATCCAG 
AAGATTCAGG CCCTGCAGAA GCGACTGATT GCTAAAACCC AGGAGCTGGA GGAACGGGGA 
CTGCTGCTGC AGGAAAAGGA GAAGCTGTAT GTGGAACTGA AGCAGGTTCT GGCCCGACGG 
CCCGGTCCCG AGGCAGCCAT GCAGCTCCAG CAGTACCGCT GGACCATCGG GGACCGGACC 
AAAAAGCTGC AGGCTCTGAT GGCAGAGCTG AGGACGGTCG ACTCAAAGAT GAACGAATAC 
AAACGGGAGA ATCAAAGACT GAGCGGCGAG CTGGCAAATA TGAAGAAGAA GTATTTGAGT 
CAGAAAAGGC GTCATAGTGA GCAGAGAAGC AGGACCACGG CGCAGGAACA GGAAGCTCTG 
CCCCAGCTGA GCAGCACGCC TCGCTTCGCC AGAGCGGCCT TCAGGGTTTA CAACCCGGTC 
AAACTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cfap58 ( XM_016673562.1 ) cDNA ORF clone, Poecilia formosa(Amazon molly) -> XP_016529048.1 Poecilia formosa cilia and flagella associated protein 58 (cfap58), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OPo140007
Clone ID Related Accession (Same CDS sequence)	XM_016673562.1
Accession Version	XM_016673562.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2139bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-05-02
Organism	Poecilia formosa(Amazon molly)
Product	cilia- and flagella-associated protein 58 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_006800018.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Poecilia formosa Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGAAA CAGAATTTGA AGGAGACGAC CGTTTTGAGT CGTTGGAGGA GTTTCAGGTT 
GTCCTCAGTG AGCTGTCCAG TGACAGCAGC CTGGACAGGA TCAGGGCGGA GTACGAGAAA 
CTCGTCCATG CTCTGAAGAA GTCGCGGGAG AATGAAAAGA GGCTGATGTC GAAATGCAGG 
GAGCTGAACG CAGAGATCGC GTCGACATCA ACCAAAGTGG CAACTGCCCT GAAACTGTCC 
CAGGAAGATG AGATGACAAT AACGTCGCTC AAGAGGGAGT TGGATAAGGT TTGGAAGTTG 
ATTGATGCAG CTCATGATAA AGAGAGAAAG GACCAGGAAA CAATCAAGGA TTTAAAAGAA 
GACGTTTTCA AGCTCACAAA GGCAACTGAG CAAAAAACCG GACATCAGCA GGACGAAGAA 
CGAATGGATT TAATTAAAAT GATTGAAGAG TTGACTAAGG AGAGAGACCA GCTGACAAAT 
GCGGCGGCGG ACCTAAGAGA GAAACTAAAC AAGGCGACTG TAACTCAGCA AGACATAGAG 
GCTCAAAAAG AGACAGCCGT AGAGAAGATC TTGAAGCTTC AGCAGGAGCT CCAGGTGCAA 
CAGAACGAGC TCTCCAGAGA GACGAGACTG AAGGAGAAGC TCGACAAGGA GATCCAGCAG 
CTGCACACCG ACATGGAGGC CAAGATGGCC GAAATCAAAG CTCTGACCAC GCAGGGACAG 
AAGGCCAGAG AGGAGCAGCA GCGACTGGAG CAGCAGCTCA GAGATCTAAA GGTATTGCAT 
GAGCGCTTGA CCAAAGAACT GGAGCAGATT CAGGCGAAGA GCAGCAAACT GCAGCAGGAA 
TGTGAACGTC TCTTGGCCGC CAGGGAACAG CTCTCTGTGG AGAATCATCT GAAGGCAAAC 
GAACTGAAGA TAAGAGAGGA GGAGCTGAGT CACATGCGTC AGGAGGTCGC CAAACAGACC 
AAGATGAGAG AAGCCATCCA GAAGAAGTAC CACCACATGG AGGACCAGAA AGCTGAACTC 
GACCGACAGA GGGAGACGCT GAAAGCTCAG ATTGCTGGCC TGGAAAAAGA TCTTGAATCT 
TCCCGGAAGC AAGTGGAAGT CGATAAAAAA GCCAAGGAGG AGCTGATCCG AGAGAGGGCC 
AATCTGCACA AGAACATGAC CAAAGCCGTG CAGTCGGCTG AGAAGCAGCA GGACCTCATG 
AAGCTCCTGG AGCAGGACAA GAGAACCTTG GAGCACGAGA TCTCCGGGTA CCGGCAGGAG 
GCGCAGAAGC AGCGGAAGAT CATCCAGCAG CTGGAGAAGG AGCGGGACCG CTACGTCAAC 
GAGAGCGGCA GCCTTATGCA GAAGGTGCAA CAGAAGCTCA CTGTTGTCGA AGACAAAGAG 
ATGGAGATAT TTGATTGGAA AAAGAAAGCT ACAGAGTCGG AGTGTAAACT CAAACAGCAG 
GAGAACCTGC TGGAGTCGAT CATAGCGGAG AGGAATCTAT ACAGCAAAAA CCTCATCGAG 
TCTCAGGAGG AGATTTCTGA AATGAAGAGG AAAATAAAGA CTATGAGCAA CCAAGTTGCC 
CGGCTTAAAG ACGACATCTC TGAGAAGGAG CAGGAACTGG TCAGAGATCA GCAGGAGCAG 
AAGAGGCTGG AGAAGGAGAA CGAAGCCTAC AAGGGGGATC TACAGGCAAT AAAACTGCAG 
CTGGAGGAAA CGAAGCAGCT TATTGACAGC CAGAGGACAG AACAGCAAAA ACTGCAGATG 
ACAATCACCA GCATGGAGGA AGACCGAGTC CAGGAGCAGA AGCAGCTGCA GCAGGCGATC 
CGAGAGCGGG ACAGCCTCGG CAAGCAGCTG CTGCAGCGGA GTGATGAGCG CGCTCTCCTG 
CTTGAGAAGA TCCGGATCCA GCAGTCCATC CTCAGCAAGG GGGACTTCCA CTACAAGCAG 
AGGCTGGAGG ACATCCAGCT GCTGAAGCTG GAGATCAAGA GGCTGCGCCG CAGCAACGAC 
ATCCAGGACA GGACCTTACC CAACACAGAG GAGCTGAGGA CGGAGCAGCT CCGCCTGCAG 
ACGGAGCTGC TCAGAGAGAG AACCAGGAAC AGTGTTCTGG AGGACCAGCT CAAGCCCATA 
AATATTCACC GATGGAGGCA ACTGGAGGTG AGGCTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016529048.1
CDS	98..2236
Translation	MEETEFEGDDRFESLEEFQVVLSELSSDSSLDRIRAEYEKLVHALKKSRENEKRLMSKCRELNAEIASTSTKVATALKLSQEDEMTITSLKRELDKVWKLIDAAHDKERKDQETIKDLKEDVFKLTKATEQKTGHQQDEERMDLIKMIEELTKERDQLTNAAADLREKLNKATVTQQDIEAQKETAVEKILKLQQELQVQQNELSRETRLKEKLDKEIQQLHTDMEAKMAEIKALTTQGQKAREEQQRLEQQLRDLKVLHERLTKELEQIQAKSSKLQQECERLLAAREQLSVENHLKANELKIREEELSHMRQEVAKQTKMREAIQKKYHHMEDQKAELDRQRETLKAQIAGLEKDLESSRKQVEVDKKAKEELIRERANLHKNMTKAVQSAEKQQDLMKLLEQDKRTLEHEISGYRQEAQKQRKIIQQLEKERDRYVNESGSLMQKVQQKLTVVEDKEMEIFDWKKKATESECKLKQQENLLESIIAERNLYSKNLIESQEEISEMKRKIKTMSNQVARLKDDISEKEQELVRDQQEQKRLEKENEAYKGDLQAIKLQLEETKQLIDSQRTEQQKLQMTITSMEEDRVQEQKQLQQAIRERDSLGKQLLQRSDERALLLEKIRIQQSILSKGDFHYKQRLEDIQLLKLEIKRLRRSNDIQDRTLPNTEELRTEQLRLQTELLRERTRNSVLEDQLKPINIHRWRQLEVRL

Target ORF information:

RefSeq Version	XM_016673562.1
Organism	Poecilia formosa(Amazon molly)
Definition	Poecilia formosa cilia and flagella associated protein 58 (cfap58), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016673562.1

ORF Insert Sequence:

ATGGAGGAAA CAGAATTTGA AGGAGACGAC CGTTTTGAGT CGTTGGAGGA GTTTCAGGTT 
GTCCTCAGTG AGCTGTCCAG TGACAGCAGC CTGGACAGGA TCAGGGCGGA GTACGAGAAA 
CTCGTCCATG CTCTGAAGAA GTCGCGGGAG AATGAAAAGA GGCTGATGTC GAAATGCAGG 
GAGCTGAACG CAGAGATCGC GTCGACATCA ACCAAAGTGG CAACTGCCCT GAAACTGTCC 
CAGGAAGATG AGATGACAAT AACGTCGCTC AAGAGGGAGT TGGATAAGGT TTGGAAGTTG 
ATTGATGCAG CTCATGATAA AGAGAGAAAG GACCAGGAAA CAATCAAGGA TTTAAAAGAA 
GACGTTTTCA AGCTCACAAA GGCAACTGAG CAAAAAACCG GACATCAGCA GGACGAAGAA 
CGAATGGATT TAATTAAAAT GATTGAAGAG TTGACTAAGG AGAGAGACCA GCTGACAAAT 
GCGGCGGCGG ACCTAAGAGA GAAACTAAAC AAGGCGACTG TAACTCAGCA AGACATAGAG 
GCTCAAAAAG AGACAGCCGT AGAGAAGATC TTGAAGCTTC AGCAGGAGCT CCAGGTGCAA 
CAGAACGAGC TCTCCAGAGA GACGAGACTG AAGGAGAAGC TCGACAAGGA GATCCAGCAG 
CTGCACACCG ACATGGAGGC CAAGATGGCC GAAATCAAAG CTCTGACCAC GCAGGGACAG 
AAGGCCAGAG AGGAGCAGCA GCGACTGGAG CAGCAGCTCA GAGATCTAAA GGTATTGCAT 
GAGCGCTTGA CCAAAGAACT GGAGCAGATT CAGGCGAAGA GCAGCAAACT GCAGCAGGAA 
TGTGAACGTC TCTTGGCCGC CAGGGAACAG CTCTCTGTGG AGAATCATCT GAAGGCAAAC 
GAACTGAAGA TAAGAGAGGA GGAGCTGAGT CACATGCGTC AGGAGGTCGC CAAACAGACC 
AAGATGAGAG AAGCCATCCA GAAGAAGTAC CACCACATGG AGGACCAGAA AGCTGAACTC 
GACCGACAGA GGGAGACGCT GAAAGCTCAG ATTGCTGGCC TGGAAAAAGA TCTTGAATCT 
TCCCGGAAGC AAGTGGAAGT CGATAAAAAA GCCAAGGAGG AGCTGATCCG AGAGAGGGCC 
AATCTGCACA AGAACATGAC CAAAGCCGTG CAGTCGGCTG AGAAGCAGCA GGACCTCATG 
AAGCTCCTGG AGCAGGACAA GAGAACCTTG GAGCACGAGA TCTCCGGGTA CCGGCAGGAG 
GCGCAGAAGC AGCGGAAGAT CATCCAGCAG CTGGAGAAGG AGCGGGACCG CTACGTCAAC 
GAGAGCGGCA GCCTTATGCA GAAGGTGCAA CAGAAGCTCA CTGTTGTCGA AGACAAAGAG 
ATGGAGATAT TTGATTGGAA AAAGAAAGCT ACAGAGTCGG AGTGTAAACT CAAACAGCAG 
GAGAACCTGC TGGAGTCGAT CATAGCGGAG AGGAATCTAT ACAGCAAAAA CCTCATCGAG 
TCTCAGGAGG AGATTTCTGA AATGAAGAGG AAAATAAAGA CTATGAGCAA CCAAGTTGCC 
CGGCTTAAAG ACGACATCTC TGAGAAGGAG CAGGAACTGG TCAGAGATCA GCAGGAGCAG 
AAGAGGCTGG AGAAGGAGAA CGAAGCCTAC AAGGGGGATC TACAGGCAAT AAAACTGCAG 
CTGGAGGAAA CGAAGCAGCT TATTGACAGC CAGAGGACAG AACAGCAAAA ACTGCAGATG 
ACAATCACCA GCATGGAGGA AGACCGAGTC CAGGAGCAGA AGCAGCTGCA GCAGGCGATC 
CGAGAGCGGG ACAGCCTCGG CAAGCAGCTG CTGCAGCGGA GTGATGAGCG CGCTCTCCTG 
CTTGAGAAGA TCCGGATCCA GCAGTCCATC CTCAGCAAGG GGGACTTCCA CTACAAGCAG 
AGGCTGGAGG ACATCCAGCT GCTGAAGCTG GAGATCAAGA GGCTGCGCCG CAGCAACGAC 
ATCCAGGACA GGACCTTACC CAACACAGAG GAGCTGAGGA CGGAGCAGCT CCGCCTGCAG 
ACGGAGCTGC TCAGAGAGAG AACCAGGAAC AGTGTTCTGG AGGACCAGCT CAAGCCCATA 
AATATTCACC GATGGAGGCA ACTGGAGGTG AGGCTTTAA