LOC103139585 cDNA ORF clone, Poecilia formosa(Amazon molly)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC103139585
Entrez Gene ID	103139585
Full Name	von Willebrand factor A domain-containing protein 7-like
Gene Type	protein-coding
Organism	Poecilia formosa(Amazon molly)

mRNA and Protein(s)

mRNA	Protein	Name
XM_007554287.2	XP_007554349.1	von Willebrand factor A domain-containing protein 7-like
XM_016672708.1	XP_016528194.1	von Willebrand factor A domain-containing protein 7-like

Pathways from BioSystems
Gene Ontology

The following LOC103139585 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC103139585 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OPo127071	XM_016672708.1 Latest version!	Poecilia formosa von Willebrand factor A domain-containing protein 7-like (LOC103139585), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
OPo127071	XM_007554287.2 Latest version!	Poecilia formosa von Willebrand factor A domain-containing protein 7-like (LOC103139585), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC103139585 ( XM_007554287.2 ) cDNA ORF clone, Poecilia formosa(Amazon molly) -> XP_007554349.1 Poecilia formosa von Willebrand factor A domain-containing protein 7-like (LOC103139585), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OPo127071
Clone ID Related Accession (Same CDS sequence)	XM_007554287.2 , XM_016672708.1
Accession Version	XM_007554287.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2802bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-05-02
Organism	Poecilia formosa(Amazon molly)
Product	von Willebrand factor A domain-containing protein 7-like
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_006799942.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On May 3, 2016 this sequence version replaced XM_007554287.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Poecilia formosa Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATCCGCT CGGTGTTTGC TGTGCTTTGT TTCATCCTGC TGCACATCGG AGCTCAGGCA 
TTTGAAATTT TGCCTGGAGA TTCTTTAAAT CACCAGGAAA TTACAGAGCA AGCGATTCTC 
AATGCAACTG TGCAGGCATG CCGCTCTCTA GCCCAGACTG AAGGAACAAG CTTCAACAAC 
CCAGCTCAGC CTTTCACTGC AGAAGGCGTT GCTGCTTCCT GCAGCGCCTC ACAGTCCTCG 
AAGGCTTTCC GTCAAGCCAT CACATCCATC AAGCTGAGGA ATATCAGAGT GGATATCCGC 
TACGCCCTCA AACCGAGCTT CCACTTTGAT GAGGAGGAGT TCGTTCAGGG AAGGAGGATC 
ATCACAGATG GAATGGTTGC TGTCAAAGCC GACAACAGGA TGGGGAATTA TGAGACCGCA 
AGGGAGAAAC TGGGAGAGAT CATGCATCCT TTGCAAGATT TCTACAGTCA CAGCAACTGG 
GTGGAGCTGG GAAACACGCT CCCAAACTCT AACCTGATTA AAGCAGAAAC CAGCATTGGC 
AACATCGCAG ATCAAAACAG AGCCACCTGT CGCAGCTGTG TTGGCAACGA CTGCACCAAC 
AACATTCTGG AGGACATCAT CGCCGGACAG ATCCTGACCT CTGGCTACTT CGGGCTTCTA 
CCTTTTGTTT CAACCAAGCC ACCTGGAAAG TGCAGTCATG GAGGATCAGT CGATCAAACC 
AGTAAGATCG AGCCAACCGG TGGGATTAAC AAGGATTCCT TCCAGTCCAG CCACGGACAT 
CTCCACACGC AAGCCGCAAA CCTGGCAGTA GCTGCAACCA GCCAGCTTCT GGAGGACATC 
CGACTGGCAG CTGGAGACCT CCCCTTCCTA CAGATGGTGG GGATCTCTAG AGGATCCAGC 
AAAGCTCTTT GTTTTGTCAC TGACACGTCA AAGAGCATGA GCGACGACAT TGAGGTGGTG 
AAGACCGTCA CGTCCACCAT CATCAACATT GAGGTGGGAA CAGAGAACGA ACCGTCCGTT 
TACATCCTCG TCCCATTCAA CGATCCAGGA TTTGGGCCAG TAATTCGTAC AACGGATGCA 
ACACTTTTCA AGGATATTGT GAACCATCTA ACAACTACTG GTGGAGGAGA TGTTGAGGAA 
ATGAGTCTTT CAGGACTGCA GCTGGCGTTA ACTAGTGCTC CATTCAACTC CGAGATCTTT 
CTCTTCACGG ATGCTCCTGC TAAAGACAAA GATTTGAAGA ACACGGTGAT TGCTCTCATA 
GAGCAGACCC AGACAGTGGT CAACTTTATG ATTACAGACT CCGTTGTTAC TAATCGCCGC 
AGGAGAAGAA ACAGCAACCT ACTGCAGACC AGAGCAATCT CAGAGTCTGA CGCTCAGCTG 
TACAGAGACC TGGCTCAGGC GTCGGGCGGT CAGGCCATCG TAGTGACGAA GGCCGAACTT 
CCTGTTGCCG CAGCAACCAT AGTCCAGTCT GTCAGCTCCT CATTGGTGAC CCTTCTGCAG 
GCGTCCAGGG ACCCGGGACA AACCGACAGT TTGACCTTTA TTGTTGACGA AACTGTTACA 
AACCCGACAG TTTACATCAC CGGCAGATCC CTCACTTTCA CTCTCACAAG CCCGACAGGT 
GTAACTCAGC AAAGCACCGA CTCCACAGGC TCATTGATCA CTTCTTCAGA GACTGTGGGA 
AACTTCATAA CGGTGCGATT ACTAAAGCAA GTTGGACAAT GGGCAATAAG AATGGTGTCA 
AACAACCCCT ACACCCTGAG GGTCGTTGGT GAGAGCTCTG TTGACTTCCT GTTTGACTTT 
GTCGAGCCGT CTCAGGGTCC TTTCGGTGGA TTCGATGCTC TCGACACGCG ACCCAAAGCA 
GGCGCCAGTG GCACTTTGTT GGTGACTTTA TCAGGAAGTG ACTCTGCTGC TGTGACAGAA 
GTTGCTCTGG TGGAATCGAC GAAGGGGTCG GAGGAGACGT TGGGAGTTGT GTCGCAGGGC 
AGCGGGAACT TTTTAGTCCA GGTTGATGTG ATGCCAACGG TGGAGTTTGT AGTGAGAGTA 
AAGGGGAGAG TTCAAGGTTC TGCTACTGAC TTTCAGAGAC AGTCGCCCAC CAACTTCAGA 
GCTTCTAATC TGACTCTTAC AGCCAACTCA GACAGCATCT TGACTGCAGG AACCATGACC 
TCTGTGCCCT TCACTGTGGC TTCAACTAGA GCCGATGAAA ACATAACCAT CCGAGCCACC 
AACTCGAGGA ATCTTGACTC GACGTTTCCG ACCGTTTTAT TGGTGGAAAG TGGGAACAGC 
ACTAATGGCA CGGTGACCCT GCTGGCTCCG CTGAGCATCC CGTCCGGATC CGACGTCATC 
CTCACCATCG TGGCCGAGAC GCCGGACGGA AGTGACACCA ACTACATTGT GCTGCGATTC 
TCTGTAGTAA ACTCGTTAAA TGATTTCACC CAACCAGCGT GTCAGCTTCT CAGCATGCAG 
TCTGGCTGTG TCGAGAACTG CAGCCTGTCC GCTTGGGTCG CCTCCGTCAG AGTGACTGAC 
GGCGACAGCG GGGCGGGCGT CGAACGCGTC AGCCTCATAC AGGGCACCGG CGTCTTCAAC 
ACCAGCCTGG AGGCCGGGAA CGAGAACGTA ACGCTGGTGT CCTACGTCGC CTCCTGCTGT 
GCGCCTGTAA TGCAGCTGCT GGTTGTGGAC CGGGTCGGCA ACGTTGGCAC ATGTCAGTTC 
ACTGCCAGGG TCTCCGGGTC TGCCGCGGCC TCGCTGTCTT TCTTTCTATG TCTCATCATC 
ATGGGGTTTG GGCAACTTTT ACTGAGTGGA TTCATGAAAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_007554349.1
CDS	32..2833
Translation	MIRSVFAVLCFILLHIGAQAFEILPGDSLNHQEITEQAILNATVQACRSLAQTEGTSFNNPAQPFTAEGVAASCSASQSSKAFRQAITSIKLRNIRVDIRYALKPSFHFDEEEFVQGRRIITDGMVAVKADNRMGNYETAREKLGEIMHPLQDFYSHSNWVELGNTLPNSNLIKAETSIGNIADQNRATCRSCVGNDCTNNILEDIIAGQILTSGYFGLLPFVSTKPPGKCSHGGSVDQTSKIEPTGGINKDSFQSSHGHLHTQAANLAVAATSQLLEDIRLAAGDLPFLQMVGISRGSSKALCFVTDTSKSMSDDIEVVKTVTSTIINIEVGTENEPSVYILVPFNDPGFGPVIRTTDATLFKDIVNHLTTTGGGDVEEMSLSGLQLALTSAPFNSEIFLFTDAPAKDKDLKNTVIALIEQTQTVVNFMITDSVVTNRRRRRNSNLLQTRAISESDAQLYRDLAQASGGQAIVVTKAELPVAAATIVQSVSSSLVTLLQASRDPGQTDSLTFIVDETVTNPTVYITGRSLTFTLTSPTGVTQQSTDSTGSLITSSETVGNFITVRLLKQVGQWAIRMVSNNPYTLRVVGESSVDFLFDFVEPSQGPFGGFDALDTRPKAGASGTLLVTLSGSDSAAVTEVALVESTKGSEETLGVVSQGSGNFLVQVDVMPTVEFVVRVKGRVQGSATDFQRQSPTNFRASNLTLTANSDSILTAGTMTSVPFTVASTRADENITIRATNSRNLDSTFPTVLLVESGNSTNGTVTLLAPLSIPSGSDVILTIVAETPDGSDTNYIVLRFSVVNSLNDFTQPACQLLSMQSGCVENCSLSAWVASVRVTDGDSGAGVERVSLIQGTGVFNTSLEAGNENVTLVSYVASCCAPVMQLLVVDRVGNVGTCQFTARVSGSAAASLSFFLCLIIMGFGQLLLSGFMK

Target ORF information:

RefSeq Version	XM_007554287.2
Organism	Poecilia formosa(Amazon molly)
Definition	Poecilia formosa von Willebrand factor A domain-containing protein 7-like (LOC103139585), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_007554287.2

ORF Insert Sequence:

ATGATCCGCT CGGTGTTTGC TGTGCTTTGT TTCATCCTGC TGCACATCGG AGCTCAGGCA 
TTTGAAATTT TGCCTGGAGA TTCTTTAAAT CACCAGGAAA TTACAGAGCA AGCGATTCTC 
AATGCAACTG TGCAGGCATG CCGCTCTCTA GCCCAGACTG AAGGAACAAG CTTCAACAAC 
CCAGCTCAGC CTTTCACTGC AGAAGGCGTT GCTGCTTCCT GCAGCGCCTC ACAGTCCTCG 
AAGGCTTTCC GTCAAGCCAT CACATCCATC AAGCTGAGGA ATATCAGAGT GGATATCCGC 
TACGCCCTCA AACCGAGCTT CCACTTTGAT GAGGAGGAGT TCGTTCAGGG AAGGAGGATC 
ATCACAGATG GAATGGTTGC TGTCAAAGCC GACAACAGGA TGGGGAATTA TGAGACCGCA 
AGGGAGAAAC TGGGAGAGAT CATGCATCCT TTGCAAGATT TCTACAGTCA CAGCAACTGG 
GTGGAGCTGG GAAACACGCT CCCAAACTCT AACCTGATTA AAGCAGAAAC CAGCATTGGC 
AACATCGCAG ATCAAAACAG AGCCACCTGT CGCAGCTGTG TTGGCAACGA CTGCACCAAC 
AACATTCTGG AGGACATCAT CGCCGGACAG ATCCTGACCT CTGGCTACTT CGGGCTTCTA 
CCTTTTGTTT CAACCAAGCC ACCTGGAAAG TGCAGTCATG GAGGATCAGT CGATCAAACC 
AGTAAGATCG AGCCAACCGG TGGGATTAAC AAGGATTCCT TCCAGTCCAG CCACGGACAT 
CTCCACACGC AAGCCGCAAA CCTGGCAGTA GCTGCAACCA GCCAGCTTCT GGAGGACATC 
CGACTGGCAG CTGGAGACCT CCCCTTCCTA CAGATGGTGG GGATCTCTAG AGGATCCAGC 
AAAGCTCTTT GTTTTGTCAC TGACACGTCA AAGAGCATGA GCGACGACAT TGAGGTGGTG 
AAGACCGTCA CGTCCACCAT CATCAACATT GAGGTGGGAA CAGAGAACGA ACCGTCCGTT 
TACATCCTCG TCCCATTCAA CGATCCAGGA TTTGGGCCAG TAATTCGTAC AACGGATGCA 
ACACTTTTCA AGGATATTGT GAACCATCTA ACAACTACTG GTGGAGGAGA TGTTGAGGAA 
ATGAGTCTTT CAGGACTGCA GCTGGCGTTA ACTAGTGCTC CATTCAACTC CGAGATCTTT 
CTCTTCACGG ATGCTCCTGC TAAAGACAAA GATTTGAAGA ACACGGTGAT TGCTCTCATA 
GAGCAGACCC AGACAGTGGT CAACTTTATG ATTACAGACT CCGTTGTTAC TAATCGCCGC 
AGGAGAAGAA ACAGCAACCT ACTGCAGACC AGAGCAATCT CAGAGTCTGA CGCTCAGCTG 
TACAGAGACC TGGCTCAGGC GTCGGGCGGT CAGGCCATCG TAGTGACGAA GGCCGAACTT 
CCTGTTGCCG CAGCAACCAT AGTCCAGTCT GTCAGCTCCT CATTGGTGAC CCTTCTGCAG 
GCGTCCAGGG ACCCGGGACA AACCGACAGT TTGACCTTTA TTGTTGACGA AACTGTTACA 
AACCCGACAG TTTACATCAC CGGCAGATCC CTCACTTTCA CTCTCACAAG CCCGACAGGT 
GTAACTCAGC AAAGCACCGA CTCCACAGGC TCATTGATCA CTTCTTCAGA GACTGTGGGA 
AACTTCATAA CGGTGCGATT ACTAAAGCAA GTTGGACAAT GGGCAATAAG AATGGTGTCA 
AACAACCCCT ACACCCTGAG GGTCGTTGGT GAGAGCTCTG TTGACTTCCT GTTTGACTTT 
GTCGAGCCGT CTCAGGGTCC TTTCGGTGGA TTCGATGCTC TCGACACGCG ACCCAAAGCA 
GGCGCCAGTG GCACTTTGTT GGTGACTTTA TCAGGAAGTG ACTCTGCTGC TGTGACAGAA 
GTTGCTCTGG TGGAATCGAC GAAGGGGTCG GAGGAGACGT TGGGAGTTGT GTCGCAGGGC 
AGCGGGAACT TTTTAGTCCA GGTTGATGTG ATGCCAACGG TGGAGTTTGT AGTGAGAGTA 
AAGGGGAGAG TTCAAGGTTC TGCTACTGAC TTTCAGAGAC AGTCGCCCAC CAACTTCAGA 
GCTTCTAATC TGACTCTTAC AGCCAACTCA GACAGCATCT TGACTGCAGG AACCATGACC 
TCTGTGCCCT TCACTGTGGC TTCAACTAGA GCCGATGAAA ACATAACCAT CCGAGCCACC 
AACTCGAGGA ATCTTGACTC GACGTTTCCG ACCGTTTTAT TGGTGGAAAG TGGGAACAGC 
ACTAATGGCA CGGTGACCCT GCTGGCTCCG CTGAGCATCC CGTCCGGATC CGACGTCATC 
CTCACCATCG TGGCCGAGAC GCCGGACGGA AGTGACACCA ACTACATTGT GCTGCGATTC 
TCTGTAGTAA ACTCGTTAAA TGATTTCACC CAACCAGCGT GTCAGCTTCT CAGCATGCAG 
TCTGGCTGTG TCGAGAACTG CAGCCTGTCC GCTTGGGTCG CCTCCGTCAG AGTGACTGAC 
GGCGACAGCG GGGCGGGCGT CGAACGCGTC AGCCTCATAC AGGGCACCGG CGTCTTCAAC 
ACCAGCCTGG AGGCCGGGAA CGAGAACGTA ACGCTGGTGT CCTACGTCGC CTCCTGCTGT 
GCGCCTGTAA TGCAGCTGCT GGTTGTGGAC CGGGTCGGCA ACGTTGGCAC ATGTCAGTTC 
ACTGCCAGGG TCTCCGGGTC TGCCGCGGCC TCGCTGTCTT TCTTTCTATG TCTCATCATC 
ATGGGGTTTG GGCAACTTTT ACTGAGTGGA TTCATGAAAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC103139585 ( XM_016672708.1 ) cDNA ORF clone, Poecilia formosa(Amazon molly) -> XP_016528194.1 Poecilia formosa von Willebrand factor A domain-containing protein 7-like (LOC103139585), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OPo127071
Clone ID Related Accession (Same CDS sequence)	XM_007554287.2 , XM_016672708.1
Accession Version	XM_016672708.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2802bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-05-02
Organism	Poecilia formosa(Amazon molly)
Product	von Willebrand factor A domain-containing protein 7-like
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_006799942.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Poecilia formosa Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATCCGCT CGGTGTTTGC TGTGCTTTGT TTCATCCTGC TGCACATCGG AGCTCAGGCA 
TTTGAAATTT TGCCTGGAGA TTCTTTAAAT CACCAGGAAA TTACAGAGCA AGCGATTCTC 
AATGCAACTG TGCAGGCATG CCGCTCTCTA GCCCAGACTG AAGGAACAAG CTTCAACAAC 
CCAGCTCAGC CTTTCACTGC AGAAGGCGTT GCTGCTTCCT GCAGCGCCTC ACAGTCCTCG 
AAGGCTTTCC GTCAAGCCAT CACATCCATC AAGCTGAGGA ATATCAGAGT GGATATCCGC 
TACGCCCTCA AACCGAGCTT CCACTTTGAT GAGGAGGAGT TCGTTCAGGG AAGGAGGATC 
ATCACAGATG GAATGGTTGC TGTCAAAGCC GACAACAGGA TGGGGAATTA TGAGACCGCA 
AGGGAGAAAC TGGGAGAGAT CATGCATCCT TTGCAAGATT TCTACAGTCA CAGCAACTGG 
GTGGAGCTGG GAAACACGCT CCCAAACTCT AACCTGATTA AAGCAGAAAC CAGCATTGGC 
AACATCGCAG ATCAAAACAG AGCCACCTGT CGCAGCTGTG TTGGCAACGA CTGCACCAAC 
AACATTCTGG AGGACATCAT CGCCGGACAG ATCCTGACCT CTGGCTACTT CGGGCTTCTA 
CCTTTTGTTT CAACCAAGCC ACCTGGAAAG TGCAGTCATG GAGGATCAGT CGATCAAACC 
AGTAAGATCG AGCCAACCGG TGGGATTAAC AAGGATTCCT TCCAGTCCAG CCACGGACAT 
CTCCACACGC AAGCCGCAAA CCTGGCAGTA GCTGCAACCA GCCAGCTTCT GGAGGACATC 
CGACTGGCAG CTGGAGACCT CCCCTTCCTA CAGATGGTGG GGATCTCTAG AGGATCCAGC 
AAAGCTCTTT GTTTTGTCAC TGACACGTCA AAGAGCATGA GCGACGACAT TGAGGTGGTG 
AAGACCGTCA CGTCCACCAT CATCAACATT GAGGTGGGAA CAGAGAACGA ACCGTCCGTT 
TACATCCTCG TCCCATTCAA CGATCCAGGA TTTGGGCCAG TAATTCGTAC AACGGATGCA 
ACACTTTTCA AGGATATTGT GAACCATCTA ACAACTACTG GTGGAGGAGA TGTTGAGGAA 
ATGAGTCTTT CAGGACTGCA GCTGGCGTTA ACTAGTGCTC CATTCAACTC CGAGATCTTT 
CTCTTCACGG ATGCTCCTGC TAAAGACAAA GATTTGAAGA ACACGGTGAT TGCTCTCATA 
GAGCAGACCC AGACAGTGGT CAACTTTATG ATTACAGACT CCGTTGTTAC TAATCGCCGC 
AGGAGAAGAA ACAGCAACCT ACTGCAGACC AGAGCAATCT CAGAGTCTGA CGCTCAGCTG 
TACAGAGACC TGGCTCAGGC GTCGGGCGGT CAGGCCATCG TAGTGACGAA GGCCGAACTT 
CCTGTTGCCG CAGCAACCAT AGTCCAGTCT GTCAGCTCCT CATTGGTGAC CCTTCTGCAG 
GCGTCCAGGG ACCCGGGACA AACCGACAGT TTGACCTTTA TTGTTGACGA AACTGTTACA 
AACCCGACAG TTTACATCAC CGGCAGATCC CTCACTTTCA CTCTCACAAG CCCGACAGGT 
GTAACTCAGC AAAGCACCGA CTCCACAGGC TCATTGATCA CTTCTTCAGA GACTGTGGGA 
AACTTCATAA CGGTGCGATT ACTAAAGCAA GTTGGACAAT GGGCAATAAG AATGGTGTCA 
AACAACCCCT ACACCCTGAG GGTCGTTGGT GAGAGCTCTG TTGACTTCCT GTTTGACTTT 
GTCGAGCCGT CTCAGGGTCC TTTCGGTGGA TTCGATGCTC TCGACACGCG ACCCAAAGCA 
GGCGCCAGTG GCACTTTGTT GGTGACTTTA TCAGGAAGTG ACTCTGCTGC TGTGACAGAA 
GTTGCTCTGG TGGAATCGAC GAAGGGGTCG GAGGAGACGT TGGGAGTTGT GTCGCAGGGC 
AGCGGGAACT TTTTAGTCCA GGTTGATGTG ATGCCAACGG TGGAGTTTGT AGTGAGAGTA 
AAGGGGAGAG TTCAAGGTTC TGCTACTGAC TTTCAGAGAC AGTCGCCCAC CAACTTCAGA 
GCTTCTAATC TGACTCTTAC AGCCAACTCA GACAGCATCT TGACTGCAGG AACCATGACC 
TCTGTGCCCT TCACTGTGGC TTCAACTAGA GCCGATGAAA ACATAACCAT CCGAGCCACC 
AACTCGAGGA ATCTTGACTC GACGTTTCCG ACCGTTTTAT TGGTGGAAAG TGGGAACAGC 
ACTAATGGCA CGGTGACCCT GCTGGCTCCG CTGAGCATCC CGTCCGGATC CGACGTCATC 
CTCACCATCG TGGCCGAGAC GCCGGACGGA AGTGACACCA ACTACATTGT GCTGCGATTC 
TCTGTAGTAA ACTCGTTAAA TGATTTCACC CAACCAGCGT GTCAGCTTCT CAGCATGCAG 
TCTGGCTGTG TCGAGAACTG CAGCCTGTCC GCTTGGGTCG CCTCCGTCAG AGTGACTGAC 
GGCGACAGCG GGGCGGGCGT CGAACGCGTC AGCCTCATAC AGGGCACCGG CGTCTTCAAC 
ACCAGCCTGG AGGCCGGGAA CGAGAACGTA ACGCTGGTGT CCTACGTCGC CTCCTGCTGT 
GCGCCTGTAA TGCAGCTGCT GGTTGTGGAC CGGGTCGGCA ACGTTGGCAC ATGTCAGTTC 
ACTGCCAGGG TCTCCGGGTC TGCCGCGGCC TCGCTGTCTT TCTTTCTATG TCTCATCATC 
ATGGGGTTTG GGCAACTTTT ACTGAGTGGA TTCATGAAAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016528194.1
CDS	88..2889
Translation	MIRSVFAVLCFILLHIGAQAFEILPGDSLNHQEITEQAILNATVQACRSLAQTEGTSFNNPAQPFTAEGVAASCSASQSSKAFRQAITSIKLRNIRVDIRYALKPSFHFDEEEFVQGRRIITDGMVAVKADNRMGNYETAREKLGEIMHPLQDFYSHSNWVELGNTLPNSNLIKAETSIGNIADQNRATCRSCVGNDCTNNILEDIIAGQILTSGYFGLLPFVSTKPPGKCSHGGSVDQTSKIEPTGGINKDSFQSSHGHLHTQAANLAVAATSQLLEDIRLAAGDLPFLQMVGISRGSSKALCFVTDTSKSMSDDIEVVKTVTSTIINIEVGTENEPSVYILVPFNDPGFGPVIRTTDATLFKDIVNHLTTTGGGDVEEMSLSGLQLALTSAPFNSEIFLFTDAPAKDKDLKNTVIALIEQTQTVVNFMITDSVVTNRRRRRNSNLLQTRAISESDAQLYRDLAQASGGQAIVVTKAELPVAAATIVQSVSSSLVTLLQASRDPGQTDSLTFIVDETVTNPTVYITGRSLTFTLTSPTGVTQQSTDSTGSLITSSETVGNFITVRLLKQVGQWAIRMVSNNPYTLRVVGESSVDFLFDFVEPSQGPFGGFDALDTRPKAGASGTLLVTLSGSDSAAVTEVALVESTKGSEETLGVVSQGSGNFLVQVDVMPTVEFVVRVKGRVQGSATDFQRQSPTNFRASNLTLTANSDSILTAGTMTSVPFTVASTRADENITIRATNSRNLDSTFPTVLLVESGNSTNGTVTLLAPLSIPSGSDVILTIVAETPDGSDTNYIVLRFSVVNSLNDFTQPACQLLSMQSGCVENCSLSAWVASVRVTDGDSGAGVERVSLIQGTGVFNTSLEAGNENVTLVSYVASCCAPVMQLLVVDRVGNVGTCQFTARVSGSAAASLSFFLCLIIMGFGQLLLSGFMK

Target ORF information:

RefSeq Version	XM_016672708.1
Organism	Poecilia formosa(Amazon molly)
Definition	Poecilia formosa von Willebrand factor A domain-containing protein 7-like (LOC103139585), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016672708.1

ORF Insert Sequence:

ATGATCCGCT CGGTGTTTGC TGTGCTTTGT TTCATCCTGC TGCACATCGG AGCTCAGGCA 
TTTGAAATTT TGCCTGGAGA TTCTTTAAAT CACCAGGAAA TTACAGAGCA AGCGATTCTC 
AATGCAACTG TGCAGGCATG CCGCTCTCTA GCCCAGACTG AAGGAACAAG CTTCAACAAC 
CCAGCTCAGC CTTTCACTGC AGAAGGCGTT GCTGCTTCCT GCAGCGCCTC ACAGTCCTCG 
AAGGCTTTCC GTCAAGCCAT CACATCCATC AAGCTGAGGA ATATCAGAGT GGATATCCGC 
TACGCCCTCA AACCGAGCTT CCACTTTGAT GAGGAGGAGT TCGTTCAGGG AAGGAGGATC 
ATCACAGATG GAATGGTTGC TGTCAAAGCC GACAACAGGA TGGGGAATTA TGAGACCGCA 
AGGGAGAAAC TGGGAGAGAT CATGCATCCT TTGCAAGATT TCTACAGTCA CAGCAACTGG 
GTGGAGCTGG GAAACACGCT CCCAAACTCT AACCTGATTA AAGCAGAAAC CAGCATTGGC 
AACATCGCAG ATCAAAACAG AGCCACCTGT CGCAGCTGTG TTGGCAACGA CTGCACCAAC 
AACATTCTGG AGGACATCAT CGCCGGACAG ATCCTGACCT CTGGCTACTT CGGGCTTCTA 
CCTTTTGTTT CAACCAAGCC ACCTGGAAAG TGCAGTCATG GAGGATCAGT CGATCAAACC 
AGTAAGATCG AGCCAACCGG TGGGATTAAC AAGGATTCCT TCCAGTCCAG CCACGGACAT 
CTCCACACGC AAGCCGCAAA CCTGGCAGTA GCTGCAACCA GCCAGCTTCT GGAGGACATC 
CGACTGGCAG CTGGAGACCT CCCCTTCCTA CAGATGGTGG GGATCTCTAG AGGATCCAGC 
AAAGCTCTTT GTTTTGTCAC TGACACGTCA AAGAGCATGA GCGACGACAT TGAGGTGGTG 
AAGACCGTCA CGTCCACCAT CATCAACATT GAGGTGGGAA CAGAGAACGA ACCGTCCGTT 
TACATCCTCG TCCCATTCAA CGATCCAGGA TTTGGGCCAG TAATTCGTAC AACGGATGCA 
ACACTTTTCA AGGATATTGT GAACCATCTA ACAACTACTG GTGGAGGAGA TGTTGAGGAA 
ATGAGTCTTT CAGGACTGCA GCTGGCGTTA ACTAGTGCTC CATTCAACTC CGAGATCTTT 
CTCTTCACGG ATGCTCCTGC TAAAGACAAA GATTTGAAGA ACACGGTGAT TGCTCTCATA 
GAGCAGACCC AGACAGTGGT CAACTTTATG ATTACAGACT CCGTTGTTAC TAATCGCCGC 
AGGAGAAGAA ACAGCAACCT ACTGCAGACC AGAGCAATCT CAGAGTCTGA CGCTCAGCTG 
TACAGAGACC TGGCTCAGGC GTCGGGCGGT CAGGCCATCG TAGTGACGAA GGCCGAACTT 
CCTGTTGCCG CAGCAACCAT AGTCCAGTCT GTCAGCTCCT CATTGGTGAC CCTTCTGCAG 
GCGTCCAGGG ACCCGGGACA AACCGACAGT TTGACCTTTA TTGTTGACGA AACTGTTACA 
AACCCGACAG TTTACATCAC CGGCAGATCC CTCACTTTCA CTCTCACAAG CCCGACAGGT 
GTAACTCAGC AAAGCACCGA CTCCACAGGC TCATTGATCA CTTCTTCAGA GACTGTGGGA 
AACTTCATAA CGGTGCGATT ACTAAAGCAA GTTGGACAAT GGGCAATAAG AATGGTGTCA 
AACAACCCCT ACACCCTGAG GGTCGTTGGT GAGAGCTCTG TTGACTTCCT GTTTGACTTT 
GTCGAGCCGT CTCAGGGTCC TTTCGGTGGA TTCGATGCTC TCGACACGCG ACCCAAAGCA 
GGCGCCAGTG GCACTTTGTT GGTGACTTTA TCAGGAAGTG ACTCTGCTGC TGTGACAGAA 
GTTGCTCTGG TGGAATCGAC GAAGGGGTCG GAGGAGACGT TGGGAGTTGT GTCGCAGGGC 
AGCGGGAACT TTTTAGTCCA GGTTGATGTG ATGCCAACGG TGGAGTTTGT AGTGAGAGTA 
AAGGGGAGAG TTCAAGGTTC TGCTACTGAC TTTCAGAGAC AGTCGCCCAC CAACTTCAGA 
GCTTCTAATC TGACTCTTAC AGCCAACTCA GACAGCATCT TGACTGCAGG AACCATGACC 
TCTGTGCCCT TCACTGTGGC TTCAACTAGA GCCGATGAAA ACATAACCAT CCGAGCCACC 
AACTCGAGGA ATCTTGACTC GACGTTTCCG ACCGTTTTAT TGGTGGAAAG TGGGAACAGC 
ACTAATGGCA CGGTGACCCT GCTGGCTCCG CTGAGCATCC CGTCCGGATC CGACGTCATC 
CTCACCATCG TGGCCGAGAC GCCGGACGGA AGTGACACCA ACTACATTGT GCTGCGATTC 
TCTGTAGTAA ACTCGTTAAA TGATTTCACC CAACCAGCGT GTCAGCTTCT CAGCATGCAG 
TCTGGCTGTG TCGAGAACTG CAGCCTGTCC GCTTGGGTCG CCTCCGTCAG AGTGACTGAC 
GGCGACAGCG GGGCGGGCGT CGAACGCGTC AGCCTCATAC AGGGCACCGG CGTCTTCAAC 
ACCAGCCTGG AGGCCGGGAA CGAGAACGTA ACGCTGGTGT CCTACGTCGC CTCCTGCTGT 
GCGCCTGTAA TGCAGCTGCT GGTTGTGGAC CGGGTCGGCA ACGTTGGCAC ATGTCAGTTC 
ACTGCCAGGG TCTCCGGGTC TGCCGCGGCC TCGCTGTCTT TCTTTCTATG TCTCATCATC 
ATGGGGTTTG GGCAACTTTT ACTGAGTGGA TTCATGAAAT GA