tyw1 cDNA ORF clone, Poecilia formosa(Amazon molly)

Gene
Clones
gRNAs

Summary

Gene Symbol	tyw1
Entrez Gene ID	103138280
Full Name	tRNA-yW synthesizing protein 1 homolog
Gene Type	protein-coding
Organism	Poecilia formosa(Amazon molly)

mRNA and Protein(s)

mRNA	Protein	Name
XM_007552412.2	XP_007552474.1	S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase

Pathways from BioSystems
Gene Ontology

The following tyw1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the tyw1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OPo136453	XM_007552412.2 Latest version!	Poecilia formosa tRNA-yW synthesizing protein 1 homolog (tyw1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

tyw1 ( XM_007552412.2 ) cDNA ORF clone, Poecilia formosa(Amazon molly) -> XP_007552474.1 Poecilia formosa tRNA-yW synthesizing protein 1 homolog (tyw1), mRNA.

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CloneID	OPo136453
Clone ID Related Accession (Same CDS sequence)	XM_007552412.2
Accession Version	XM_007552412.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2244bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-05-02
Organism	Poecilia formosa(Amazon molly)
Product	S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_006799987.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On May 3, 2016 this sequence version replaced XM_007552412.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Poecilia formosa Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTAGCTT TGTTTACTGT TATTTCCTTT TTTACGGGCT CTTTGTCTGC CTCCGACACT 
AAAGTCCCTC CGGATGTCCA CAGTCCCACT GAAAACTATC TGCTGTGGAT GTGGAACAAC 
AGACTCTACG TTTATTCTGC TGCTGCGCTG CTGCTGGGTT TCTGCTTCCT ACTGCAGGTT 
ACACGAAGGA AGATAAATCA TACAAAAGAT TCTGGTTCTA AGGATTCTGG TCTGAAGGTT 
CAAAAAGCTG CAAAAAACAG CTCAGATGAA GATGCTGATG TTCATGTTTC AGGGGTTAAA 
ATCTTCTACG GCTCACAAAC TGGGACAGCA AAGGGTTTTG CCTCAGAGCT CTCACAGGAG 
GTGCAGGCTC TGGGTATCCC AGCGGAGGTG ATAGACATGA AGGACTTCGA CCCAGACGAC 
CGACTTGCAG ACGAGTGCGT CAGTAAGTCC GTCTGCGTGT TCCTCGTGGC CACGTACACG 
GACGGACGGC CCACCCAGAA CGCCGAGTGG TTCTGCAAGT GGCTGGAAGA AGCGTCCACC 
GACTTCCGCT ACGGCAACTC CTTCCTCAAG GGGCTCCGCT ACGCCGTGTT CGGCCTCGGC 
AACTCCGTCT ACGTCGGCCA CTACAACACG GTGGGAACAA ACGTCGACAA GTGGCTGTGG 
ATGCTGAGCG CCAGGCGAGT GTTGAGCAGA GGAGAGGGCG ACGGCAACGT GGTGAAGAGC 
CGACACGGCA GCGTTCAGGC CGACTTCCGG GCCTGGAAGG TCCGGTTCCT GAGCCGCCTC 
AGGGCTCTGG CCAGAGGGGA GAAGAAGAGC TGCAGCGGGA ACTGCAAGAG CGGCGCTTCC 
TGCAAGAACC GGAAGAAGCA CGGAGCGCCG GAGGAGGAGG AGCACGGATC CGAGGTGGAG 
CTGATTGAAT CCAGCAGTGA GGAGGAAGAG TCCGGCTGGC CGGATGAGAA AACGTCGCGC 
TCTGTGGTCG ACGTGGAGGA TCTGGGCAAC ATCATGAACA GCGTCAAGAA AGCAAAGGGT 
GTGAAAGGGG AACAAGGAGG CCAAATGGTG AAACTGTCCA AACACAATGG AGTGATGAAG 
ACTGAAGATG AAGAGGAGAG GAGGGAGATG ATCACCCCGG CTCTGAGGGA AGCACTGACC 
AAGCAAGGTT ATAAGTTAAT TGGAAGTCAC TCCGGAGTGA AGCTTTGTCG GTGGACAAAG 
TCTATGCTGC GAGGGAGAGG AGGTTGTTAC AAACACACGT TTTACGGCAT CGAGTCGCAT 
CGCTGCATGG AGACGACGCC CAGCCTGGCC TGCGCAAACA AATGCGTCTT CTGCTGGAGA 
CACCACACCA ACCCGGTTGG CACCGAGTGG CGCTGGAAAA TGGACCCGGC AGAGAAAATC 
CTGCAGGATT CCCTGGAGAA GCACCAGAAC ATGATCCGGC AGTTCAGAGG CGTCCCCGGG 
GTGAAGCCGG AGCGATACGA GGAGGGTTTG GCCGCCAAGC ACTGCGCCCT GTCCTTGGTA 
GGCGAGCCAA TCATGTACCC AGAAATCAAC ACCTTTCTGC GCCTCCTCCA CTCTCATCGC 
ATCTCCAGCT TCCTGGTCAC AAACGCTCAG TTCCCAGAGG AAATCAGGAA CCTGGTGCCG 
GTGACTCAGC TGTACGTCAG TGTGGATGCC AGCACCAAGG ACAGCCTGAA GAAGATTGAT 
CGGCCTCTTT TTAAGGACTT CTGGCCTCGT TTCCTCGACT GCCTCAGGGC TCTGGGAGAG 
AAAAAGCAGA GGACGGTTTA CCGACTGACG CTGGTGAAAG CCTGGAACGT GGAGGAGCTG 
CAGGCCTACG CCGAGCTCAT CGCTCTGGGC CAGCCCGACT TCATCGAGGT CAAGGGCGTG 
ACGTACTGCG GGGAAAGCTC GGCCAGCAGT CTGACCATGG CGAACGTCCC CTGGCACCAG 
GAAGTGGTCG CCTTCGTCCA GCAGCTGGCT GACATGCTGC CTCACTACCA GATCGCCTGC 
GAGCACGAGC ACTCCAACTG TTTGCTCATC GCACACAGGA AGTTCAAGGT GAACGGCGAG 
TGGCGGACTT GGATCGACTA CGACCGTTTC CAGGATCTGG TCCAGGCTCA CGAAGACAGC 
GGCGGACAGC AGACCTTCTC AGCCTTGGAC TACACCGCCA AGACCCCCAG CTGGGCTTTG 
TTTGGGTCAA ACGAACAAGG GTTCGACCCG GCCGACACGC GCTTCCAGCG GCGCAACAAA 
ACCAAAGATA TCTCTGGGTG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_007552474.1
CDS	87..2330
Translation	MLALFTVISFFTGSLSASDTKVPPDVHSPTENYLLWMWNNRLYVYSAAALLLGFCFLLQVTRRKINHTKDSGSKDSGLKVQKAAKNSSDEDADVHVSGVKIFYGSQTGTAKGFASELSQEVQALGIPAEVIDMKDFDPDDRLADECVSKSVCVFLVATYTDGRPTQNAEWFCKWLEEASTDFRYGNSFLKGLRYAVFGLGNSVYVGHYNTVGTNVDKWLWMLSARRVLSRGEGDGNVVKSRHGSVQADFRAWKVRFLSRLRALARGEKKSCSGNCKSGASCKNRKKHGAPEEEEHGSEVELIESSSEEEESGWPDEKTSRSVVDVEDLGNIMNSVKKAKGVKGEQGGQMVKLSKHNGVMKTEDEEERREMITPALREALTKQGYKLIGSHSGVKLCRWTKSMLRGRGGCYKHTFYGIESHRCMETTPSLACANKCVFCWRHHTNPVGTEWRWKMDPAEKILQDSLEKHQNMIRQFRGVPGVKPERYEEGLAAKHCALSLVGEPIMYPEINTFLRLLHSHRISSFLVTNAQFPEEIRNLVPVTQLYVSVDASTKDSLKKIDRPLFKDFWPRFLDCLRALGEKKQRTVYRLTLVKAWNVEELQAYAELIALGQPDFIEVKGVTYCGESSASSLTMANVPWHQEVVAFVQQLADMLPHYQIACEHEHSNCLLIAHRKFKVNGEWRTWIDYDRFQDLVQAHEDSGGQQTFSALDYTAKTPSWALFGSNEQGFDPADTRFQRRNKTKDISGC

Target ORF information:

RefSeq Version	XM_007552412.2
Organism	Poecilia formosa(Amazon molly)
Definition	Poecilia formosa tRNA-yW synthesizing protein 1 homolog (tyw1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_007552412.2

ORF Insert Sequence:

ATGTTAGCTT TGTTTACTGT TATTTCCTTT TTTACGGGCT CTTTGTCTGC CTCCGACACT 
AAAGTCCCTC CGGATGTCCA CAGTCCCACT GAAAACTATC TGCTGTGGAT GTGGAACAAC 
AGACTCTACG TTTATTCTGC TGCTGCGCTG CTGCTGGGTT TCTGCTTCCT ACTGCAGGTT 
ACACGAAGGA AGATAAATCA TACAAAAGAT TCTGGTTCTA AGGATTCTGG TCTGAAGGTT 
CAAAAAGCTG CAAAAAACAG CTCAGATGAA GATGCTGATG TTCATGTTTC AGGGGTTAAA 
ATCTTCTACG GCTCACAAAC TGGGACAGCA AAGGGTTTTG CCTCAGAGCT CTCACAGGAG 
GTGCAGGCTC TGGGTATCCC AGCGGAGGTG ATAGACATGA AGGACTTCGA CCCAGACGAC 
CGACTTGCAG ACGAGTGCGT CAGTAAGTCC GTCTGCGTGT TCCTCGTGGC CACGTACACG 
GACGGACGGC CCACCCAGAA CGCCGAGTGG TTCTGCAAGT GGCTGGAAGA AGCGTCCACC 
GACTTCCGCT ACGGCAACTC CTTCCTCAAG GGGCTCCGCT ACGCCGTGTT CGGCCTCGGC 
AACTCCGTCT ACGTCGGCCA CTACAACACG GTGGGAACAA ACGTCGACAA GTGGCTGTGG 
ATGCTGAGCG CCAGGCGAGT GTTGAGCAGA GGAGAGGGCG ACGGCAACGT GGTGAAGAGC 
CGACACGGCA GCGTTCAGGC CGACTTCCGG GCCTGGAAGG TCCGGTTCCT GAGCCGCCTC 
AGGGCTCTGG CCAGAGGGGA GAAGAAGAGC TGCAGCGGGA ACTGCAAGAG CGGCGCTTCC 
TGCAAGAACC GGAAGAAGCA CGGAGCGCCG GAGGAGGAGG AGCACGGATC CGAGGTGGAG 
CTGATTGAAT CCAGCAGTGA GGAGGAAGAG TCCGGCTGGC CGGATGAGAA AACGTCGCGC 
TCTGTGGTCG ACGTGGAGGA TCTGGGCAAC ATCATGAACA GCGTCAAGAA AGCAAAGGGT 
GTGAAAGGGG AACAAGGAGG CCAAATGGTG AAACTGTCCA AACACAATGG AGTGATGAAG 
ACTGAAGATG AAGAGGAGAG GAGGGAGATG ATCACCCCGG CTCTGAGGGA AGCACTGACC 
AAGCAAGGTT ATAAGTTAAT TGGAAGTCAC TCCGGAGTGA AGCTTTGTCG GTGGACAAAG 
TCTATGCTGC GAGGGAGAGG AGGTTGTTAC AAACACACGT TTTACGGCAT CGAGTCGCAT 
CGCTGCATGG AGACGACGCC CAGCCTGGCC TGCGCAAACA AATGCGTCTT CTGCTGGAGA 
CACCACACCA ACCCGGTTGG CACCGAGTGG CGCTGGAAAA TGGACCCGGC AGAGAAAATC 
CTGCAGGATT CCCTGGAGAA GCACCAGAAC ATGATCCGGC AGTTCAGAGG CGTCCCCGGG 
GTGAAGCCGG AGCGATACGA GGAGGGTTTG GCCGCCAAGC ACTGCGCCCT GTCCTTGGTA 
GGCGAGCCAA TCATGTACCC AGAAATCAAC ACCTTTCTGC GCCTCCTCCA CTCTCATCGC 
ATCTCCAGCT TCCTGGTCAC AAACGCTCAG TTCCCAGAGG AAATCAGGAA CCTGGTGCCG 
GTGACTCAGC TGTACGTCAG TGTGGATGCC AGCACCAAGG ACAGCCTGAA GAAGATTGAT 
CGGCCTCTTT TTAAGGACTT CTGGCCTCGT TTCCTCGACT GCCTCAGGGC TCTGGGAGAG 
AAAAAGCAGA GGACGGTTTA CCGACTGACG CTGGTGAAAG CCTGGAACGT GGAGGAGCTG 
CAGGCCTACG CCGAGCTCAT CGCTCTGGGC CAGCCCGACT TCATCGAGGT CAAGGGCGTG 
ACGTACTGCG GGGAAAGCTC GGCCAGCAGT CTGACCATGG CGAACGTCCC CTGGCACCAG 
GAAGTGGTCG CCTTCGTCCA GCAGCTGGCT GACATGCTGC CTCACTACCA GATCGCCTGC 
GAGCACGAGC ACTCCAACTG TTTGCTCATC GCACACAGGA AGTTCAAGGT GAACGGCGAG 
TGGCGGACTT GGATCGACTA CGACCGTTTC CAGGATCTGG TCCAGGCTCA CGAAGACAGC 
GGCGGACAGC AGACCTTCTC AGCCTTGGAC TACACCGCCA AGACCCCCAG CTGGGCTTTG 
TTTGGGTCAA ACGAACAAGG GTTCGACCCG GCCGACACGC GCTTCCAGCG GCGCAACAAA 
ACCAAAGATA TCTCTGGGTG CTGA