LOC103135855 cDNA ORF clone, Poecilia formosa(Amazon molly)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC103135855
Entrez Gene ID	103135855
Full Name	3-hydroxy-3-methylglutaryl-coenzyme A reductase-like
Gene Type	protein-coding
Organism	Poecilia formosa(Amazon molly)

mRNA and Protein(s)

mRNA	Protein	Name
XM_007548805.2	XP_007548867.1	3-hydroxy-3-methylglutaryl-coenzyme A reductase-like
XM_007548806.2	XP_007548868.1	3-hydroxy-3-methylglutaryl-coenzyme A reductase-like

Pathways from BioSystems
Gene Ontology

The following LOC103135855 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC103135855 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OPo133236	XM_007548805.2 Latest version!	Poecilia formosa 3-hydroxy-3-methylglutaryl-coenzyme A reductase-like (LOC103135855), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
OPo133236	XM_007548806.2 Latest version!	Poecilia formosa 3-hydroxy-3-methylglutaryl-coenzyme A reductase-like (LOC103135855), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC103135855 ( XM_007548805.2 ) cDNA ORF clone, Poecilia formosa(Amazon molly) -> XP_007548867.1 Poecilia formosa 3-hydroxy-3-methylglutaryl-coenzyme A reductase-like (LOC103135855), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OPo133236
Clone ID Related Accession (Same CDS sequence)	XM_007548805.2 , XM_007548806.2
Accession Version	XM_007548805.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2604bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-05-02
Organism	Poecilia formosa(Amazon molly)
Product	3-hydroxy-3-methylglutaryl-coenzyme A reductase-like
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_006799967.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On May 3, 2016 this sequence version replaced XM_007548805.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Poecilia formosa Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGGCCC GTCTTTTCCG CCTCCACGGC CTGCTGGTGG CCTCCCACCC CTGGGAGGTG 
ATAGTGGGGA CCCTGGCCGT CACCGTCTGC CTCATGAACA TGAACAGCTC ATCGACCAGC 
AGTCAGATGT GCAGCTGGAA CAACTGTCCA AAGGTTAAAG AGGAGGCCCC CAGTAGTGAT 
ATAATCGTTT TGACCATCAC TCGCTGCATG GCTATTATTT ATATCTACTT CCAGTTTAAG 
AACCTCCTGC AGCTGGGATC CAAATATATT CTGGGTATTG CTGGATTGTT TACAGTGTTT 
TCCAGCTTTG TTTTCAGTAC AGTGGTCATC CACTTCTTCG GGAAAGAACT AACCGGTCTT 
AATGAAGCTC TGCCGTTCTT TCTCCTGCTC ATTGACCTGT CCAAAGCCTG CACTTTAGCT 
AAATTTGCCC TCAGCTCCAA CTCCCAGGAG GAGGTGAGGG AGAACATATC CAAGGGCATG 
GCTATCCTCG GCCCCACGTT CACCCTGGAT GCTCTCGTCG AGTGTCTGGT TATAGGAATC 
GGCACCATGT CGGGCGTTCC TCAGTTGGAG ATCATGTGCT GTTTTGGCTG CATGTCTGTT 
CTGGCCAATT ACTTTGTTTT CATGACGTTC TTCCCAGCGT GCGTCTCTCT AGTCCTCGAG 
CTGTCCAGGG AAAGTCGGGA AGGCCGTCCC ATCTGGCAGA TGAGCCACTT GGCTAATGTT 
CTGGCAGAGG AAGAAGACAA CAAGCCCAAC CCTGTGACCC AAAGAGTTAA AATCATCATG 
TCTCTCGGTT TGGCCCTGGT CCACGCCCAC ACTCGGTTAA CAGCCGAGCA TTCGGGTCGC 
AATCGCACAG CAGAGGGACC CGTAGCTGAG AGACTGGAAT CAGCTGGGAC CGTGTGGCCC 
CTGAAACTCA CCAGCATGGA GCTGGAACAT GTGATAACCC TTGGCCTGGC GCTGCTTCTC 
GCCATCAAGT ACGTCTTCTT TGAGCAAGCG GACACCGAGT CCTCCCTGTC TCTCAGGAGT 
CCCATTAGCG GCGCCTCTCT GGTTCAGAAG CCACGGGCAG CCGACAGCTG CTGCAGGAGG 
GACCAATCAG CTGCCAGGAA AAGCTCTGCT GGTGTGTTAG CAACCGGGGT TGCTTCCTCG 
GCGGTCTCAG ACGTTAAACC GTTGCCTGAG GCTGACATTA CCTGTAAAGG ATCATTCGGC 
GGCTGTGCTT CACCTCGCCT GGAGACGTGC AGAGCTCCTG CAGGCTGTGG AGCAGAACCT 
CGCTCACTGG AGGAATGCAT GGCCGTCCTC TCTGACCCCC AGAGGGGTCC AGAAATGCTG 
ACGGATTCAG AGGTGATCAA CCTGGTGACC GCTAGGAAGA TCCTGAACTA CAAACTGGAA 
GCGCTGCTGG AGACTCCAGA AAGGGGCGTG GCCATCAGGA GAGAGATGCT GGCGCTGAAA 
CTTCCTGTCA GCTCCGCTTT GTCTGGTCTG CCTTACAAAG GCTACGACTA TTCAAAGGTG 
ATGGGCGCTT GCTGCGAGAA CGTCATCGGC TACATGCCAG TGCCAGTGGG CGTGGCCGGC 
CCGCTGCTGT TGGACGGCCA GCAGTTTCAT GTCCCCATGG CGACCACGGA GGGCTGCCTC 
GTGGCCAGCA CCAACAGAGG ATGCAGAGCG GTTTCTCTGA GCGGGGGCTG CAGCAGCAGG 
ATCCTGGCCG ACAGCATGAC CAGGGGTCCT GTGGTGAGGC TGCCTTCGGC GTGCCGGGCC 
GCAGAGGTCA AAATGTGGCT GGAGACGACG GATGGGTTCG GCTTGGTCAA GGAAGCCTTC 
GACGAGACCA GCAGGTTTGC CCGTTTGGAG AAGCTGCTGG CCTGTGTGGC AGGAAGAAAC 
CTTTACATTC GCTTCCAGTC GCAGACGGGC GACGCCATGG GGATGAACAT GCTCTCTAAG 
GGGACGGAGC AGGCTCTGGG TCGGCTCAAG CAGCACTTCC CAGACATGGA GGTTCTGTCT 
GTCAGTGGTA ACTACTGCAC TGACAAAAAG TCTGCTGCCA TCAACTGGAT CCTGGGTCGG 
GGGAAGTCCG CTGTGTGTGA AGCCACCATC CCTGCCCAGG TGGTGAGGGA GGTTCTGAAG 
AGCAGCACGG CCGCTCTGGT GGATCTGAAC ATCAGTAAGA ACCTGGTGGG CTCGGCCATG 
GCCGGCAGCA TAGGGGGCTT CAACGCACAT GCCGCCAACA TCGTCAGCGC CATCTTCATC 
GCCTGTGGAC AGGACCCGGC TCAGACCGTG GGGAGCTCCA GCTGCATCAC TCAGATGGAG 
GCTGCCGGCC CAGACGGAGA CGACCTGTAC ATCAGCTGCA CCATGCCGTC CATAGAGCTG 
GGCACTGTGG GCGGAGGCAC CAACCTGCCG CCACAGCAGG CCTGTCTGCA GATGCTTGGT 
GTCGCCGGGC CCAACCAGCC AGGAGAGAAC GCCCGCCAGC TGGCCCGCGT AGTGTGTGCC 
ACCGTCCTGG CGGGGGAGCT GTCTCTGATG GCCGCTCTGG CAGCAGGACA CCTCGTCAAA 
AGCCACATGA CTCACAACAG ATCTAAGACC AGCCTGCCCG AGATGGCTTT CCCAGCGACC 
GAGACAAACG TCCCTAAGTG TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_007548867.1
CDS	175..2778
Translation	MLARLFRLHGLLVASHPWEVIVGTLAVTVCLMNMNSSSTSSQMCSWNNCPKVKEEAPSSDIIVLTITRCMAIIYIYFQFKNLLQLGSKYILGIAGLFTVFSSFVFSTVVIHFFGKELTGLNEALPFFLLLIDLSKACTLAKFALSSNSQEEVRENISKGMAILGPTFTLDALVECLVIGIGTMSGVPQLEIMCCFGCMSVLANYFVFMTFFPACVSLVLELSRESREGRPIWQMSHLANVLAEEEDNKPNPVTQRVKIIMSLGLALVHAHTRLTAEHSGRNRTAEGPVAERLESAGTVWPLKLTSMELEHVITLGLALLLAIKYVFFEQADTESSLSLRSPISGASLVQKPRAADSCCRRDQSAARKSSAGVLATGVASSAVSDVKPLPEADITCKGSFGGCASPRLETCRAPAGCGAEPRSLEECMAVLSDPQRGPEMLTDSEVINLVTARKILNYKLEALLETPERGVAIRREMLALKLPVSSALSGLPYKGYDYSKVMGACCENVIGYMPVPVGVAGPLLLDGQQFHVPMATTEGCLVASTNRGCRAVSLSGGCSSRILADSMTRGPVVRLPSACRAAEVKMWLETTDGFGLVKEAFDETSRFARLEKLLACVAGRNLYIRFQSQTGDAMGMNMLSKGTEQALGRLKQHFPDMEVLSVSGNYCTDKKSAAINWILGRGKSAVCEATIPAQVVREVLKSSTAALVDLNISKNLVGSAMAGSIGGFNAHAANIVSAIFIACGQDPAQTVGSSSCITQMEAAGPDGDDLYISCTMPSIELGTVGGGTNLPPQQACLQMLGVAGPNQPGENARQLARVVCATVLAGELSLMAALAAGHLVKSHMTHNRSKTSLPEMAFPATETNVPKC

Target ORF information:

RefSeq Version	XM_007548805.2
Organism	Poecilia formosa(Amazon molly)
Definition	Poecilia formosa 3-hydroxy-3-methylglutaryl-coenzyme A reductase-like (LOC103135855), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_007548805.2

ORF Insert Sequence:

ATGCTGGCCC GTCTTTTCCG CCTCCACGGC CTGCTGGTGG CCTCCCACCC CTGGGAGGTG 
ATAGTGGGGA CCCTGGCCGT CACCGTCTGC CTCATGAACA TGAACAGCTC ATCGACCAGC 
AGTCAGATGT GCAGCTGGAA CAACTGTCCA AAGGTTAAAG AGGAGGCCCC CAGTAGTGAT 
ATAATCGTTT TGACCATCAC TCGCTGCATG GCTATTATTT ATATCTACTT CCAGTTTAAG 
AACCTCCTGC AGCTGGGATC CAAATATATT CTGGGTATTG CTGGATTGTT TACAGTGTTT 
TCCAGCTTTG TTTTCAGTAC AGTGGTCATC CACTTCTTCG GGAAAGAACT AACCGGTCTT 
AATGAAGCTC TGCCGTTCTT TCTCCTGCTC ATTGACCTGT CCAAAGCCTG CACTTTAGCT 
AAATTTGCCC TCAGCTCCAA CTCCCAGGAG GAGGTGAGGG AGAACATATC CAAGGGCATG 
GCTATCCTCG GCCCCACGTT CACCCTGGAT GCTCTCGTCG AGTGTCTGGT TATAGGAATC 
GGCACCATGT CGGGCGTTCC TCAGTTGGAG ATCATGTGCT GTTTTGGCTG CATGTCTGTT 
CTGGCCAATT ACTTTGTTTT CATGACGTTC TTCCCAGCGT GCGTCTCTCT AGTCCTCGAG 
CTGTCCAGGG AAAGTCGGGA AGGCCGTCCC ATCTGGCAGA TGAGCCACTT GGCTAATGTT 
CTGGCAGAGG AAGAAGACAA CAAGCCCAAC CCTGTGACCC AAAGAGTTAA AATCATCATG 
TCTCTCGGTT TGGCCCTGGT CCACGCCCAC ACTCGGTTAA CAGCCGAGCA TTCGGGTCGC 
AATCGCACAG CAGAGGGACC CGTAGCTGAG AGACTGGAAT CAGCTGGGAC CGTGTGGCCC 
CTGAAACTCA CCAGCATGGA GCTGGAACAT GTGATAACCC TTGGCCTGGC GCTGCTTCTC 
GCCATCAAGT ACGTCTTCTT TGAGCAAGCG GACACCGAGT CCTCCCTGTC TCTCAGGAGT 
CCCATTAGCG GCGCCTCTCT GGTTCAGAAG CCACGGGCAG CCGACAGCTG CTGCAGGAGG 
GACCAATCAG CTGCCAGGAA AAGCTCTGCT GGTGTGTTAG CAACCGGGGT TGCTTCCTCG 
GCGGTCTCAG ACGTTAAACC GTTGCCTGAG GCTGACATTA CCTGTAAAGG ATCATTCGGC 
GGCTGTGCTT CACCTCGCCT GGAGACGTGC AGAGCTCCTG CAGGCTGTGG AGCAGAACCT 
CGCTCACTGG AGGAATGCAT GGCCGTCCTC TCTGACCCCC AGAGGGGTCC AGAAATGCTG 
ACGGATTCAG AGGTGATCAA CCTGGTGACC GCTAGGAAGA TCCTGAACTA CAAACTGGAA 
GCGCTGCTGG AGACTCCAGA AAGGGGCGTG GCCATCAGGA GAGAGATGCT GGCGCTGAAA 
CTTCCTGTCA GCTCCGCTTT GTCTGGTCTG CCTTACAAAG GCTACGACTA TTCAAAGGTG 
ATGGGCGCTT GCTGCGAGAA CGTCATCGGC TACATGCCAG TGCCAGTGGG CGTGGCCGGC 
CCGCTGCTGT TGGACGGCCA GCAGTTTCAT GTCCCCATGG CGACCACGGA GGGCTGCCTC 
GTGGCCAGCA CCAACAGAGG ATGCAGAGCG GTTTCTCTGA GCGGGGGCTG CAGCAGCAGG 
ATCCTGGCCG ACAGCATGAC CAGGGGTCCT GTGGTGAGGC TGCCTTCGGC GTGCCGGGCC 
GCAGAGGTCA AAATGTGGCT GGAGACGACG GATGGGTTCG GCTTGGTCAA GGAAGCCTTC 
GACGAGACCA GCAGGTTTGC CCGTTTGGAG AAGCTGCTGG CCTGTGTGGC AGGAAGAAAC 
CTTTACATTC GCTTCCAGTC GCAGACGGGC GACGCCATGG GGATGAACAT GCTCTCTAAG 
GGGACGGAGC AGGCTCTGGG TCGGCTCAAG CAGCACTTCC CAGACATGGA GGTTCTGTCT 
GTCAGTGGTA ACTACTGCAC TGACAAAAAG TCTGCTGCCA TCAACTGGAT CCTGGGTCGG 
GGGAAGTCCG CTGTGTGTGA AGCCACCATC CCTGCCCAGG TGGTGAGGGA GGTTCTGAAG 
AGCAGCACGG CCGCTCTGGT GGATCTGAAC ATCAGTAAGA ACCTGGTGGG CTCGGCCATG 
GCCGGCAGCA TAGGGGGCTT CAACGCACAT GCCGCCAACA TCGTCAGCGC CATCTTCATC 
GCCTGTGGAC AGGACCCGGC TCAGACCGTG GGGAGCTCCA GCTGCATCAC TCAGATGGAG 
GCTGCCGGCC CAGACGGAGA CGACCTGTAC ATCAGCTGCA CCATGCCGTC CATAGAGCTG 
GGCACTGTGG GCGGAGGCAC CAACCTGCCG CCACAGCAGG CCTGTCTGCA GATGCTTGGT 
GTCGCCGGGC CCAACCAGCC AGGAGAGAAC GCCCGCCAGC TGGCCCGCGT AGTGTGTGCC 
ACCGTCCTGG CGGGGGAGCT GTCTCTGATG GCCGCTCTGG CAGCAGGACA CCTCGTCAAA 
AGCCACATGA CTCACAACAG ATCTAAGACC AGCCTGCCCG AGATGGCTTT CCCAGCGACC 
GAGACAAACG TCCCTAAGTG TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC103135855 ( XM_007548806.2 ) cDNA ORF clone, Poecilia formosa(Amazon molly) -> XP_007548868.1 Poecilia formosa 3-hydroxy-3-methylglutaryl-coenzyme A reductase-like (LOC103135855), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OPo133236
Clone ID Related Accession (Same CDS sequence)	XM_007548805.2 , XM_007548806.2
Accession Version	XM_007548806.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2604bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-05-02
Organism	Poecilia formosa(Amazon molly)
Product	3-hydroxy-3-methylglutaryl-coenzyme A reductase-like
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_006799967.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On May 3, 2016 this sequence version replaced XM_007548806.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Poecilia formosa Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGGCCC GTCTTTTCCG CCTCCACGGC CTGCTGGTGG CCTCCCACCC CTGGGAGGTG 
ATAGTGGGGA CCCTGGCCGT CACCGTCTGC CTCATGAACA TGAACAGCTC ATCGACCAGC 
AGTCAGATGT GCAGCTGGAA CAACTGTCCA AAGGTTAAAG AGGAGGCCCC CAGTAGTGAT 
ATAATCGTTT TGACCATCAC TCGCTGCATG GCTATTATTT ATATCTACTT CCAGTTTAAG 
AACCTCCTGC AGCTGGGATC CAAATATATT CTGGGTATTG CTGGATTGTT TACAGTGTTT 
TCCAGCTTTG TTTTCAGTAC AGTGGTCATC CACTTCTTCG GGAAAGAACT AACCGGTCTT 
AATGAAGCTC TGCCGTTCTT TCTCCTGCTC ATTGACCTGT CCAAAGCCTG CACTTTAGCT 
AAATTTGCCC TCAGCTCCAA CTCCCAGGAG GAGGTGAGGG AGAACATATC CAAGGGCATG 
GCTATCCTCG GCCCCACGTT CACCCTGGAT GCTCTCGTCG AGTGTCTGGT TATAGGAATC 
GGCACCATGT CGGGCGTTCC TCAGTTGGAG ATCATGTGCT GTTTTGGCTG CATGTCTGTT 
CTGGCCAATT ACTTTGTTTT CATGACGTTC TTCCCAGCGT GCGTCTCTCT AGTCCTCGAG 
CTGTCCAGGG AAAGTCGGGA AGGCCGTCCC ATCTGGCAGA TGAGCCACTT GGCTAATGTT 
CTGGCAGAGG AAGAAGACAA CAAGCCCAAC CCTGTGACCC AAAGAGTTAA AATCATCATG 
TCTCTCGGTT TGGCCCTGGT CCACGCCCAC ACTCGGTTAA CAGCCGAGCA TTCGGGTCGC 
AATCGCACAG CAGAGGGACC CGTAGCTGAG AGACTGGAAT CAGCTGGGAC CGTGTGGCCC 
CTGAAACTCA CCAGCATGGA GCTGGAACAT GTGATAACCC TTGGCCTGGC GCTGCTTCTC 
GCCATCAAGT ACGTCTTCTT TGAGCAAGCG GACACCGAGT CCTCCCTGTC TCTCAGGAGT 
CCCATTAGCG GCGCCTCTCT GGTTCAGAAG CCACGGGCAG CCGACAGCTG CTGCAGGAGG 
GACCAATCAG CTGCCAGGAA AAGCTCTGCT GGTGTGTTAG CAACCGGGGT TGCTTCCTCG 
GCGGTCTCAG ACGTTAAACC GTTGCCTGAG GCTGACATTA CCTGTAAAGG ATCATTCGGC 
GGCTGTGCTT CACCTCGCCT GGAGACGTGC AGAGCTCCTG CAGGCTGTGG AGCAGAACCT 
CGCTCACTGG AGGAATGCAT GGCCGTCCTC TCTGACCCCC AGAGGGGTCC AGAAATGCTG 
ACGGATTCAG AGGTGATCAA CCTGGTGACC GCTAGGAAGA TCCTGAACTA CAAACTGGAA 
GCGCTGCTGG AGACTCCAGA AAGGGGCGTG GCCATCAGGA GAGAGATGCT GGCGCTGAAA 
CTTCCTGTCA GCTCCGCTTT GTCTGGTCTG CCTTACAAAG GCTACGACTA TTCAAAGGTG 
ATGGGCGCTT GCTGCGAGAA CGTCATCGGC TACATGCCAG TGCCAGTGGG CGTGGCCGGC 
CCGCTGCTGT TGGACGGCCA GCAGTTTCAT GTCCCCATGG CGACCACGGA GGGCTGCCTC 
GTGGCCAGCA CCAACAGAGG ATGCAGAGCG GTTTCTCTGA GCGGGGGCTG CAGCAGCAGG 
ATCCTGGCCG ACAGCATGAC CAGGGGTCCT GTGGTGAGGC TGCCTTCGGC GTGCCGGGCC 
GCAGAGGTCA AAATGTGGCT GGAGACGACG GATGGGTTCG GCTTGGTCAA GGAAGCCTTC 
GACGAGACCA GCAGGTTTGC CCGTTTGGAG AAGCTGCTGG CCTGTGTGGC AGGAAGAAAC 
CTTTACATTC GCTTCCAGTC GCAGACGGGC GACGCCATGG GGATGAACAT GCTCTCTAAG 
GGGACGGAGC AGGCTCTGGG TCGGCTCAAG CAGCACTTCC CAGACATGGA GGTTCTGTCT 
GTCAGTGGTA ACTACTGCAC TGACAAAAAG TCTGCTGCCA TCAACTGGAT CCTGGGTCGG 
GGGAAGTCCG CTGTGTGTGA AGCCACCATC CCTGCCCAGG TGGTGAGGGA GGTTCTGAAG 
AGCAGCACGG CCGCTCTGGT GGATCTGAAC ATCAGTAAGA ACCTGGTGGG CTCGGCCATG 
GCCGGCAGCA TAGGGGGCTT CAACGCACAT GCCGCCAACA TCGTCAGCGC CATCTTCATC 
GCCTGTGGAC AGGACCCGGC TCAGACCGTG GGGAGCTCCA GCTGCATCAC TCAGATGGAG 
GCTGCCGGCC CAGACGGAGA CGACCTGTAC ATCAGCTGCA CCATGCCGTC CATAGAGCTG 
GGCACTGTGG GCGGAGGCAC CAACCTGCCG CCACAGCAGG CCTGTCTGCA GATGCTTGGT 
GTCGCCGGGC CCAACCAGCC AGGAGAGAAC GCCCGCCAGC TGGCCCGCGT AGTGTGTGCC 
ACCGTCCTGG CGGGGGAGCT GTCTCTGATG GCCGCTCTGG CAGCAGGACA CCTCGTCAAA 
AGCCACATGA CTCACAACAG ATCTAAGACC AGCCTGCCCG AGATGGCTTT CCCAGCGACC 
GAGACAAACG TCCCTAAGTG TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_007548868.1
CDS	153..2756
Translation	MLARLFRLHGLLVASHPWEVIVGTLAVTVCLMNMNSSSTSSQMCSWNNCPKVKEEAPSSDIIVLTITRCMAIIYIYFQFKNLLQLGSKYILGIAGLFTVFSSFVFSTVVIHFFGKELTGLNEALPFFLLLIDLSKACTLAKFALSSNSQEEVRENISKGMAILGPTFTLDALVECLVIGIGTMSGVPQLEIMCCFGCMSVLANYFVFMTFFPACVSLVLELSRESREGRPIWQMSHLANVLAEEEDNKPNPVTQRVKIIMSLGLALVHAHTRLTAEHSGRNRTAEGPVAERLESAGTVWPLKLTSMELEHVITLGLALLLAIKYVFFEQADTESSLSLRSPISGASLVQKPRAADSCCRRDQSAARKSSAGVLATGVASSAVSDVKPLPEADITCKGSFGGCASPRLETCRAPAGCGAEPRSLEECMAVLSDPQRGPEMLTDSEVINLVTARKILNYKLEALLETPERGVAIRREMLALKLPVSSALSGLPYKGYDYSKVMGACCENVIGYMPVPVGVAGPLLLDGQQFHVPMATTEGCLVASTNRGCRAVSLSGGCSSRILADSMTRGPVVRLPSACRAAEVKMWLETTDGFGLVKEAFDETSRFARLEKLLACVAGRNLYIRFQSQTGDAMGMNMLSKGTEQALGRLKQHFPDMEVLSVSGNYCTDKKSAAINWILGRGKSAVCEATIPAQVVREVLKSSTAALVDLNISKNLVGSAMAGSIGGFNAHAANIVSAIFIACGQDPAQTVGSSSCITQMEAAGPDGDDLYISCTMPSIELGTVGGGTNLPPQQACLQMLGVAGPNQPGENARQLARVVCATVLAGELSLMAALAAGHLVKSHMTHNRSKTSLPEMAFPATETNVPKC

Target ORF information:

RefSeq Version	XM_007548806.2
Organism	Poecilia formosa(Amazon molly)
Definition	Poecilia formosa 3-hydroxy-3-methylglutaryl-coenzyme A reductase-like (LOC103135855), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_007548806.2

ORF Insert Sequence:

ATGCTGGCCC GTCTTTTCCG CCTCCACGGC CTGCTGGTGG CCTCCCACCC CTGGGAGGTG 
ATAGTGGGGA CCCTGGCCGT CACCGTCTGC CTCATGAACA TGAACAGCTC ATCGACCAGC 
AGTCAGATGT GCAGCTGGAA CAACTGTCCA AAGGTTAAAG AGGAGGCCCC CAGTAGTGAT 
ATAATCGTTT TGACCATCAC TCGCTGCATG GCTATTATTT ATATCTACTT CCAGTTTAAG 
AACCTCCTGC AGCTGGGATC CAAATATATT CTGGGTATTG CTGGATTGTT TACAGTGTTT 
TCCAGCTTTG TTTTCAGTAC AGTGGTCATC CACTTCTTCG GGAAAGAACT AACCGGTCTT 
AATGAAGCTC TGCCGTTCTT TCTCCTGCTC ATTGACCTGT CCAAAGCCTG CACTTTAGCT 
AAATTTGCCC TCAGCTCCAA CTCCCAGGAG GAGGTGAGGG AGAACATATC CAAGGGCATG 
GCTATCCTCG GCCCCACGTT CACCCTGGAT GCTCTCGTCG AGTGTCTGGT TATAGGAATC 
GGCACCATGT CGGGCGTTCC TCAGTTGGAG ATCATGTGCT GTTTTGGCTG CATGTCTGTT 
CTGGCCAATT ACTTTGTTTT CATGACGTTC TTCCCAGCGT GCGTCTCTCT AGTCCTCGAG 
CTGTCCAGGG AAAGTCGGGA AGGCCGTCCC ATCTGGCAGA TGAGCCACTT GGCTAATGTT 
CTGGCAGAGG AAGAAGACAA CAAGCCCAAC CCTGTGACCC AAAGAGTTAA AATCATCATG 
TCTCTCGGTT TGGCCCTGGT CCACGCCCAC ACTCGGTTAA CAGCCGAGCA TTCGGGTCGC 
AATCGCACAG CAGAGGGACC CGTAGCTGAG AGACTGGAAT CAGCTGGGAC CGTGTGGCCC 
CTGAAACTCA CCAGCATGGA GCTGGAACAT GTGATAACCC TTGGCCTGGC GCTGCTTCTC 
GCCATCAAGT ACGTCTTCTT TGAGCAAGCG GACACCGAGT CCTCCCTGTC TCTCAGGAGT 
CCCATTAGCG GCGCCTCTCT GGTTCAGAAG CCACGGGCAG CCGACAGCTG CTGCAGGAGG 
GACCAATCAG CTGCCAGGAA AAGCTCTGCT GGTGTGTTAG CAACCGGGGT TGCTTCCTCG 
GCGGTCTCAG ACGTTAAACC GTTGCCTGAG GCTGACATTA CCTGTAAAGG ATCATTCGGC 
GGCTGTGCTT CACCTCGCCT GGAGACGTGC AGAGCTCCTG CAGGCTGTGG AGCAGAACCT 
CGCTCACTGG AGGAATGCAT GGCCGTCCTC TCTGACCCCC AGAGGGGTCC AGAAATGCTG 
ACGGATTCAG AGGTGATCAA CCTGGTGACC GCTAGGAAGA TCCTGAACTA CAAACTGGAA 
GCGCTGCTGG AGACTCCAGA AAGGGGCGTG GCCATCAGGA GAGAGATGCT GGCGCTGAAA 
CTTCCTGTCA GCTCCGCTTT GTCTGGTCTG CCTTACAAAG GCTACGACTA TTCAAAGGTG 
ATGGGCGCTT GCTGCGAGAA CGTCATCGGC TACATGCCAG TGCCAGTGGG CGTGGCCGGC 
CCGCTGCTGT TGGACGGCCA GCAGTTTCAT GTCCCCATGG CGACCACGGA GGGCTGCCTC 
GTGGCCAGCA CCAACAGAGG ATGCAGAGCG GTTTCTCTGA GCGGGGGCTG CAGCAGCAGG 
ATCCTGGCCG ACAGCATGAC CAGGGGTCCT GTGGTGAGGC TGCCTTCGGC GTGCCGGGCC 
GCAGAGGTCA AAATGTGGCT GGAGACGACG GATGGGTTCG GCTTGGTCAA GGAAGCCTTC 
GACGAGACCA GCAGGTTTGC CCGTTTGGAG AAGCTGCTGG CCTGTGTGGC AGGAAGAAAC 
CTTTACATTC GCTTCCAGTC GCAGACGGGC GACGCCATGG GGATGAACAT GCTCTCTAAG 
GGGACGGAGC AGGCTCTGGG TCGGCTCAAG CAGCACTTCC CAGACATGGA GGTTCTGTCT 
GTCAGTGGTA ACTACTGCAC TGACAAAAAG TCTGCTGCCA TCAACTGGAT CCTGGGTCGG 
GGGAAGTCCG CTGTGTGTGA AGCCACCATC CCTGCCCAGG TGGTGAGGGA GGTTCTGAAG 
AGCAGCACGG CCGCTCTGGT GGATCTGAAC ATCAGTAAGA ACCTGGTGGG CTCGGCCATG 
GCCGGCAGCA TAGGGGGCTT CAACGCACAT GCCGCCAACA TCGTCAGCGC CATCTTCATC 
GCCTGTGGAC AGGACCCGGC TCAGACCGTG GGGAGCTCCA GCTGCATCAC TCAGATGGAG 
GCTGCCGGCC CAGACGGAGA CGACCTGTAC ATCAGCTGCA CCATGCCGTC CATAGAGCTG 
GGCACTGTGG GCGGAGGCAC CAACCTGCCG CCACAGCAGG CCTGTCTGCA GATGCTTGGT 
GTCGCCGGGC CCAACCAGCC AGGAGAGAAC GCCCGCCAGC TGGCCCGCGT AGTGTGTGCC 
ACCGTCCTGG CGGGGGAGCT GTCTCTGATG GCCGCTCTGG CAGCAGGACA CCTCGTCAAA 
AGCCACATGA CTCACAACAG ATCTAAGACC AGCCTGCCCG AGATGGCTTT CCCAGCGACC 
GAGACAAACG TCCCTAAGTG TTGA