nwd1 cDNA ORF clone, Poecilia formosa(Amazon molly)

Gene
Clones
gRNAs

Summary

Gene Symbol	nwd1
Entrez Gene ID	103132583
Full Name	NACHT and WD repeat domain containing 1
Gene Type	protein-coding
Organism	Poecilia formosa(Amazon molly)

mRNA and Protein(s)

mRNA	Protein	Name
XM_007544398.2	XP_007544460.2	NACHT domain- and WD repeat-containing protein 1 isoform X2
XM_016667463.1	XP_016522949.1	NACHT domain- and WD repeat-containing protein 1 isoform X1

Pathways from BioSystems
Gene Ontology

The following nwd1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the nwd1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OPo164607	XM_016667463.1 Latest version!	Poecilia formosa NACHT and WD repeat domain containing 1 (nwd1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OPo164608	XM_007544398.2 Latest version!	Poecilia formosa NACHT and WD repeat domain containing 1 (nwd1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

nwd1 ( XM_016667463.1 ) cDNA ORF clone, Poecilia formosa(Amazon molly) -> XP_016522949.1 Poecilia formosa NACHT and WD repeat domain containing 1 (nwd1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OPo164607
Clone ID Related Accession (Same CDS sequence)	XM_016667463.1
Accession Version	XM_016667463.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5115bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-05-02
Organism	Poecilia formosa(Amazon molly)
Product	NACHT domain- and WD repeat-containing protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_006801081.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Poecilia formosa Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGATCT TCAGAGCAGA ACCTTCAGCA GCGGCGCCGA CCAGTCCAGC TCGGTTCTGC 
AGCTTCATCA GAACCCATAA ACAGAAACAC GGAGCTCCGA GGGGAGAAAA GTGCCCAGGG 
AAACAAAGAG CTGCAGGAGC GCCGACAGAC AGAACCAGAA CCAGAACCGG CAGATGGTCT 
GAGACCATGG ATGGAGGACA AAGCTCCGGC GCGCGGGCCG TCCTGAGGGG ACGCTGCGAA 
GGAGCTCAGA GGACCGGCAG CAACATGATC CGAATCTTCA TCAGCTCCAG CTTCACAGAC 
ATGAGTATTG AGAGGAAGGT TCTGCTGGAG AAAGCGTACC CACAGGTCCG GTCTTTCTGC 
CGCAGTCTGG GTCTGGTCTT TGAGGTGGTG GATCTGAACT GGGGGATCCG GACCTTCCCA 
TATGGAGACC ATGAGGTCAG TGAGATCTTC CTGCAGGAGA TCCAGATGAG TCAGAAGGTT 
TCTGCTGGAC CGGCCTTCGT GGGCCTGCTG GGGAACCAGT ACGGTCACCG ACCTCTTCCT 
CGTCTCATCC CAGAAAAGCA GTTTGAGGTT CTTCTGTCCA CGCTGTCCAA GAATCCAGAA 
GGTGTTCAGC AACTGCAAGA GTGGTTTCGG AAGGACAACA ACTCTGTGCC ACCCACCTAT 
GTTCTCCAGC CAGTCACTGC ACGATTCCCA CACTACGATG ACCTCCGACC TGAGGTGACA 
AAGCTCCATG ACGATGACCT TCTGTCCTGG CGGGTCACGG AGAGTCAGCT GCTGCAGCTG 
CTACGCTCTG CAGCCGCTGA GGCTGAGACG GCTGGAGACA TCACAGCCGA GCAGAAGCAG 
CTCTTCTACT CATCAGGTGA GATATGTCCC CCGGCATGGA CATGTCCCCA AGCTTGTCCT 
TTTGGTCCAG GAGGACTAAC ATTTCCTGCT TCCCATTCAG TCACAGAACG GGCGCTGGAG 
GAAGGCGTCT GGAAGGACAA CTGCCAAATG TCCGCCTTGC TCTTCGTCCG GGAGATTCCT 
CGGCAAAGGA TGAAGGACGG ATCGAAACGT CTGGCCAAGT TCATGGACCT CACTGCAGAC 
GGGCTGCTGG ATGCTGAAGC TCAGGAGTTC CTCACCGGCC TGAAGTCACG TCTGTATGCC 
ACGTCCAAGA AGATCCTCAA CCTGCACTGC GTGGAGCTGA ACAGCGGAAG CATCGACCCA 
AAGCGCAAGG AACACGCCCA GTACCTGGAC AGCATCTGCC AGCAGTTCAT CTCCCAGATG 
ACGATCCGGA TCAGTGAGGC TGTCAAACTG GCGGGGGAGG ACAGGAAGAA GATCTGGGGA 
AGCACAGAGG AAAATGAGGA GGAGTTTGAC TGGGTGGTTG AAGAGGCGAG GCAGCACACC 
ATCATGGCTG CTGAGTTGAG CAATAGTCTC CAGGGCAGAG ATGGGCTTCT GGGGAAGATC 
TGCCTTGCCA TGTGGGAGTC CACCCTGGTC CACCACGGTC CGCTGGTGGT TCACGGCACT 
GCTGGGATGG GGAAGACCAC TCTGCTGTGC AAACTGGCAC AGGAGATGCA GGACGTCCTG 
GAGGCCAAGG CGGTGCTGGT TATTAGGCTG CTGGCTGCCA ATCATCCTCA GAGACCAAAC 
ATCAACCATG TCCTCAACAG CGTCTGCCTC CAGGTGTGTC TGGCGTGTGG CCTGGCGCCA 
CGGCCCCTGA TGTCGGTCAG CTCTCACCTG GAGCTGCAGC GGCTCTTCCA GAACATCCTG 
GAGAAGGTTT CTCAGCAGGG GAACACTCTG CTCCTCATCC TGGACTCCCT GGAGCAGCTG 
GCAGACCAAC ATCAGGCTCA CCAGTTGCGC TGGCTGCCCA CAGACGTGCC TCCAAATGTC 
CACCTGCTGG TCTCCATGGA AACCACCAGC GAGGCGTTTG CCAGCATGCG GCTGAGGCTG 
GGCACTGCAG GAAACGTCTT TGAGGTGGAG CGTTTGTCTC GTGATGAAGG GCAGCAGATC 
ATGGAGTCCC ATCTGCAGGT GGCTAAGCGA ACTTTGACCC GGGAGCAGAA GGACGCGGTG 
CTGCAGAGCT TCCATGCCTC CGGCTCTCCT CTCCACCTTC AGCTCATCCT GTCTGCTGCC 
AAGAACTGGA CATCCTTCAC ACCACCAACA GAGCTGCTGC TGGGCTCCAG CTCAGAGGAA 
GTGATGTCAC AGCTCCTCCT GAAGCTGGAG GAGAAACATG GGAAAGAGCT GGTGGGCGGG 
GCCTTGGGAT GCATTGTGTT GGCAAGGAGA GGCCTGATGG AGGCGGAACT GCGTGATGTC 
ATGTCATTGG ACGATGATGT CATCAGTGAG GTATACAGGT ATTCCCTGCC TCCAACACCT 
TCATTAATCC GGATGCCACC TGTCCTGTGG GCGCGGCTCA AGCGGGACCT GCGGGACCAG 
CTGGAGATCC AGTGGACCGA CGGAGTCGCC ATGCTGGCCT TCAGCAGCCG GCTTCTCTCA 
GAGGTAGTCA AAGCCCGCTA TCTGACACCA GAGCGACGGG GGCGGACCCA CAGGATCCTG 
GCGGAGTATT TCCTGGGTCG CTGGTCTGGA AGACTGAAGC CGGTGGCTCT TCCGGGACTG 
ACGCTGCTAC TGTCCGACAG GAAGGTCCCG CCCCAGCCGC TGTGGTTTGC TCCTGGATTG 
GCGAATGTCC GGAAGCTCCA GGAGTTGCCA TATCACTTGC TGCATGCCGG TCTGTGGGAG 
GAGCTACGGC AGGAAGTAGT AGGCAGCGCC GAGTGGCTCT ACTGTCAGAG CCGGGTCTGT 
GGGGTGTCCA GCCTGATCAG TGACCTGGAC CAGTGCTCCC AGTACATGGA CTGCTGTGAA 
ACTGGACTGG TCCATAAGGC TCTGGTCCTG ATGAAGCCCA GCCTGGACAT CCTGTCCGGC 
CACATGGACC GGTCTGTGTT TTACTCCGAG CTGCTGGCCC GACTCTCCTC CCTGGCCTCC 
ACCTCCCGCA CCGTGATTGG TCAGCTCTGC AGCCAGTGCG ACGATTGGCT CCAAACGTGT 
CCGGAGCCGG TTCTGATCCC GAGGAGCAGC TTCCTGCAGC AGCCAGGGGG GGCGCTGCAG 
CACACGCTGA CTGCACCACA CGGAGGTGTG CTCTGCTTGG CTGTGGGCGC GGCGGTGCGG 
CTGCTGGTCA CCGGCTCGGC CGGCGGCGTG GTGGCGGTCT GGAGCCTCCT GGACAAACAT 
CTCCTGCAGG TTCTGGAGGG ACCATCAGCG GCCATCTTGT CAGTAAAGAT CATCGAAAGC 
TCCGCCCACT GCCTCTCATT GGCTGCAGAT GGCTCCCTGA GGGGGTGGAG CTTAAAGAGT 
GGCCAGCAGC TGTTCTGCAT CCAGGAGGCG GGGCCTGCTG GCTCCGGCCC TTCCTCAGTG 
AGCCTGCAGG TGTCGGACCA GCTGCTGTTT GTTCACACCT GGATGCAGGT GAAGGTCTGG 
AAGTTGGACG GAACCGAGAT CCACAGAGAG GACATCTCGT TGGTTTTGGG GGTTCTGGGA 
GATTTTGTGG TATCTCTGCA TGACTCGGAC CGGGTCAGAA TCTTTGACCC GGTTACTGGG 
ACCCAGACGA TGGTGCGGCT GACCCGGAAC CTAACTCCAG TGGTTTCAGT GACCTCACCT 
AAACGTGGGT CATTATTTGT GGTGTCAGAC GACGGGTTCC TGCATCAGAT CTCCAGGACA 
AGACAGCAAA TCGTCACAGA GTTTCCTCTG CTTCCATCTC TTTTCTCAGC TTCAGAGGAT 
GAGAAGATCC TGATAGCAGG AAGGGAGCGG ACTCTGAGCC TGTTCCGTAT CGGCAGCAAC 
TCTGTGGACG GATTCCTGGA GCTGCAGCAC GATGATGAGG TTTTATCTGC CTGCGTGTCG 
CCGGACGGCC GACGCGTCGT CTCTGGAGCG GCGGATCAGC TGATCCGAAT CTGGTCGGTG 
ACCACCGGCC ACCTGGTGGA CACTCTGTGC GGCTGCAACG CTCCGGTCAC CGCCCTGATG 
CTCTACGACC ACTTCGTGGT CTCGGCCTCC TCGGCGGCGG ATTCGGTCCA GCTGTGGAGT 
CTGACCTACG ACCCCAGCCA CAAGCCCCTC CCACACATCC CGGCAGGCTC CGCCCACTCA 
GCCGTCACCA GAGACGGAGA TCAAGTGTTC TATGTGCGCC ACCAGACCCA GAGGGAAGTG 
CTGAGCTGGA ACAACCGCAC AGGTTCCGTG TCGGAGCGCC TGCCAGTGTC TGCGGAGGTC 
AGCTGCCTGG AGCTGGCGCA GCAGAAGCGC CTCCTGCTGT GCGGGCTGCG TAGCGGCACG 
GTGCTGATCT ACCCTCTGGC GTTGCCCCAG GAGACGCTGT GCATCCCGCC GCCAGAGGGC 
CTGGCCCCAG TGCTCTGCCT GGCCCTCGGC TCCCAGGAGA GCCTGCTGGC CGTCGCCCAC 
TGGGACACGC TGCGTCTGTT CCAGGTCGCC ACCAGAGACG GCTTCCCCGC CGTGGAGGGG 
CCCCTGGCGA CGGCCCCGCT GCTCCACGCC CCCCCGTCCC ACATGGCGCT GCTGCCCCAC 
CGCCGGCTGC TGTACGGGAC GGCCGGTGGC GAGGTGAAGC TGCACGACTT CGGCGCCGGC 
GCCGGCGCCG ACCTGCAGCC TCACAGCTCC GGGATCACCT GCGTCACGGC CAGCAACTGG 
GGGACGCACG CCCTGGTGGG CTCCCAGGAC GCCACACTGC GGCTGTGGGC CCTGGACCCG 
GTGGCCCTGG ACCACACCAT GGAGTACCAG GGGCTTTTCT TCCAGGGCGT CCTCTGCGCT 
GCCTTCGCCG ACAGCGACCG CTTCGTCTTC ACCGGATCTC AGGACCAAAC CGTCAAGGTC 
TGGGACGTCG CTTCAGGGAA GCTGCTGTGC GTCCAGTTCG TCTACTCGCC GGTCGTCCGC 
ATGCTGACCT TCAGGAATGG CTTCGTGGGC CTCACCCTGC AGGGCTGCGT CGTCCAGGAG 
GCCTTCAGGT GTCCGGACCG GGTCGACCCG GACCACAACC CTCTGAGGAA CATGAAGGCC 
CGGTACCGGG TCACCTCCAG GGAGAAGGGC GGGGCCGGCC GCTCCGACCT GCAGGGCTTC 
GACCCGGCCC AGATCAGCCT GAACCTAGTG AGCATGCTCA GCAGCAAGCC GTCCTCCGCC 
TGCGTCCTGC TGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016522949.1
CDS	112..5226
Translation	MLIFRAEPSAAAPTSPARFCSFIRTHKQKHGAPRGEKCPGKQRAAGAPTDRTRTRTGRWSETMDGGQSSGARAVLRGRCEGAQRTGSNMIRIFISSSFTDMSIERKVLLEKAYPQVRSFCRSLGLVFEVVDLNWGIRTFPYGDHEVSEIFLQEIQMSQKVSAGPAFVGLLGNQYGHRPLPRLIPEKQFEVLLSTLSKNPEGVQQLQEWFRKDNNSVPPTYVLQPVTARFPHYDDLRPEVTKLHDDDLLSWRVTESQLLQLLRSAAAEAETAGDITAEQKQLFYSSGEICPPAWTCPQACPFGPGGLTFPASHSVTERALEEGVWKDNCQMSALLFVREIPRQRMKDGSKRLAKFMDLTADGLLDAEAQEFLTGLKSRLYATSKKILNLHCVELNSGSIDPKRKEHAQYLDSICQQFISQMTIRISEAVKLAGEDRKKIWGSTEENEEEFDWVVEEARQHTIMAAELSNSLQGRDGLLGKICLAMWESTLVHHGPLVVHGTAGMGKTTLLCKLAQEMQDVLEAKAVLVIRLLAANHPQRPNINHVLNSVCLQVCLACGLAPRPLMSVSSHLELQRLFQNILEKVSQQGNTLLLILDSLEQLADQHQAHQLRWLPTDVPPNVHLLVSMETTSEAFASMRLRLGTAGNVFEVERLSRDEGQQIMESHLQVAKRTLTREQKDAVLQSFHASGSPLHLQLILSAAKNWTSFTPPTELLLGSSSEEVMSQLLLKLEEKHGKELVGGALGCIVLARRGLMEAELRDVMSLDDDVISEVYRYSLPPTPSLIRMPPVLWARLKRDLRDQLEIQWTDGVAMLAFSSRLLSEVVKARYLTPERRGRTHRILAEYFLGRWSGRLKPVALPGLTLLLSDRKVPPQPLWFAPGLANVRKLQELPYHLLHAGLWEELRQEVVGSAEWLYCQSRVCGVSSLISDLDQCSQYMDCCETGLVHKALVLMKPSLDILSGHMDRSVFYSELLARLSSLASTSRTVIGQLCSQCDDWLQTCPEPVLIPRSSFLQQPGGALQHTLTAPHGGVLCLAVGAAVRLLVTGSAGGVVAVWSLLDKHLLQVLEGPSAAILSVKIIESSAHCLSLAADGSLRGWSLKSGQQLFCIQEAGPAGSGPSSVSLQVSDQLLFVHTWMQVKVWKLDGTEIHREDISLVLGVLGDFVVSLHDSDRVRIFDPVTGTQTMVRLTRNLTPVVSVTSPKRGSLFVVSDDGFLHQISRTRQQIVTEFPLLPSLFSASEDEKILIAGRERTLSLFRIGSNSVDGFLELQHDDEVLSACVSPDGRRVVSGAADQLIRIWSVTTGHLVDTLCGCNAPVTALMLYDHFVVSASSAADSVQLWSLTYDPSHKPLPHIPAGSAHSAVTRDGDQVFYVRHQTQREVLSWNNRTGSVSERLPVSAEVSCLELAQQKRLLLCGLRSGTVLIYPLALPQETLCIPPPEGLAPVLCLALGSQESLLAVAHWDTLRLFQVATRDGFPAVEGPLATAPLLHAPPSHMALLPHRRLLYGTAGGEVKLHDFGAGAGADLQPHSSGITCVTASNWGTHALVGSQDATLRLWALDPVALDHTMEYQGLFFQGVLCAAFADSDRFVFTGSQDQTVKVWDVASGKLLCVQFVYSPVVRMLTFRNGFVGLTLQGCVVQEAFRCPDRVDPDHNPLRNMKARYRVTSREKGGAGRSDLQGFDPAQISLNLVSMLSSKPSSACVLL

Target ORF information:

RefSeq Version	XM_016667463.1
Organism	Poecilia formosa(Amazon molly)
Definition	Poecilia formosa NACHT and WD repeat domain containing 1 (nwd1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016667463.1

ORF Insert Sequence:

ATGCTGATCT TCAGAGCAGA ACCTTCAGCA GCGGCGCCGA CCAGTCCAGC TCGGTTCTGC 
AGCTTCATCA GAACCCATAA ACAGAAACAC GGAGCTCCGA GGGGAGAAAA GTGCCCAGGG 
AAACAAAGAG CTGCAGGAGC GCCGACAGAC AGAACCAGAA CCAGAACCGG CAGATGGTCT 
GAGACCATGG ATGGAGGACA AAGCTCCGGC GCGCGGGCCG TCCTGAGGGG ACGCTGCGAA 
GGAGCTCAGA GGACCGGCAG CAACATGATC CGAATCTTCA TCAGCTCCAG CTTCACAGAC 
ATGAGTATTG AGAGGAAGGT TCTGCTGGAG AAAGCGTACC CACAGGTCCG GTCTTTCTGC 
CGCAGTCTGG GTCTGGTCTT TGAGGTGGTG GATCTGAACT GGGGGATCCG GACCTTCCCA 
TATGGAGACC ATGAGGTCAG TGAGATCTTC CTGCAGGAGA TCCAGATGAG TCAGAAGGTT 
TCTGCTGGAC CGGCCTTCGT GGGCCTGCTG GGGAACCAGT ACGGTCACCG ACCTCTTCCT 
CGTCTCATCC CAGAAAAGCA GTTTGAGGTT CTTCTGTCCA CGCTGTCCAA GAATCCAGAA 
GGTGTTCAGC AACTGCAAGA GTGGTTTCGG AAGGACAACA ACTCTGTGCC ACCCACCTAT 
GTTCTCCAGC CAGTCACTGC ACGATTCCCA CACTACGATG ACCTCCGACC TGAGGTGACA 
AAGCTCCATG ACGATGACCT TCTGTCCTGG CGGGTCACGG AGAGTCAGCT GCTGCAGCTG 
CTACGCTCTG CAGCCGCTGA GGCTGAGACG GCTGGAGACA TCACAGCCGA GCAGAAGCAG 
CTCTTCTACT CATCAGGTGA GATATGTCCC CCGGCATGGA CATGTCCCCA AGCTTGTCCT 
TTTGGTCCAG GAGGACTAAC ATTTCCTGCT TCCCATTCAG TCACAGAACG GGCGCTGGAG 
GAAGGCGTCT GGAAGGACAA CTGCCAAATG TCCGCCTTGC TCTTCGTCCG GGAGATTCCT 
CGGCAAAGGA TGAAGGACGG ATCGAAACGT CTGGCCAAGT TCATGGACCT CACTGCAGAC 
GGGCTGCTGG ATGCTGAAGC TCAGGAGTTC CTCACCGGCC TGAAGTCACG TCTGTATGCC 
ACGTCCAAGA AGATCCTCAA CCTGCACTGC GTGGAGCTGA ACAGCGGAAG CATCGACCCA 
AAGCGCAAGG AACACGCCCA GTACCTGGAC AGCATCTGCC AGCAGTTCAT CTCCCAGATG 
ACGATCCGGA TCAGTGAGGC TGTCAAACTG GCGGGGGAGG ACAGGAAGAA GATCTGGGGA 
AGCACAGAGG AAAATGAGGA GGAGTTTGAC TGGGTGGTTG AAGAGGCGAG GCAGCACACC 
ATCATGGCTG CTGAGTTGAG CAATAGTCTC CAGGGCAGAG ATGGGCTTCT GGGGAAGATC 
TGCCTTGCCA TGTGGGAGTC CACCCTGGTC CACCACGGTC CGCTGGTGGT TCACGGCACT 
GCTGGGATGG GGAAGACCAC TCTGCTGTGC AAACTGGCAC AGGAGATGCA GGACGTCCTG 
GAGGCCAAGG CGGTGCTGGT TATTAGGCTG CTGGCTGCCA ATCATCCTCA GAGACCAAAC 
ATCAACCATG TCCTCAACAG CGTCTGCCTC CAGGTGTGTC TGGCGTGTGG CCTGGCGCCA 
CGGCCCCTGA TGTCGGTCAG CTCTCACCTG GAGCTGCAGC GGCTCTTCCA GAACATCCTG 
GAGAAGGTTT CTCAGCAGGG GAACACTCTG CTCCTCATCC TGGACTCCCT GGAGCAGCTG 
GCAGACCAAC ATCAGGCTCA CCAGTTGCGC TGGCTGCCCA CAGACGTGCC TCCAAATGTC 
CACCTGCTGG TCTCCATGGA AACCACCAGC GAGGCGTTTG CCAGCATGCG GCTGAGGCTG 
GGCACTGCAG GAAACGTCTT TGAGGTGGAG CGTTTGTCTC GTGATGAAGG GCAGCAGATC 
ATGGAGTCCC ATCTGCAGGT GGCTAAGCGA ACTTTGACCC GGGAGCAGAA GGACGCGGTG 
CTGCAGAGCT TCCATGCCTC CGGCTCTCCT CTCCACCTTC AGCTCATCCT GTCTGCTGCC 
AAGAACTGGA CATCCTTCAC ACCACCAACA GAGCTGCTGC TGGGCTCCAG CTCAGAGGAA 
GTGATGTCAC AGCTCCTCCT GAAGCTGGAG GAGAAACATG GGAAAGAGCT GGTGGGCGGG 
GCCTTGGGAT GCATTGTGTT GGCAAGGAGA GGCCTGATGG AGGCGGAACT GCGTGATGTC 
ATGTCATTGG ACGATGATGT CATCAGTGAG GTATACAGGT ATTCCCTGCC TCCAACACCT 
TCATTAATCC GGATGCCACC TGTCCTGTGG GCGCGGCTCA AGCGGGACCT GCGGGACCAG 
CTGGAGATCC AGTGGACCGA CGGAGTCGCC ATGCTGGCCT TCAGCAGCCG GCTTCTCTCA 
GAGGTAGTCA AAGCCCGCTA TCTGACACCA GAGCGACGGG GGCGGACCCA CAGGATCCTG 
GCGGAGTATT TCCTGGGTCG CTGGTCTGGA AGACTGAAGC CGGTGGCTCT TCCGGGACTG 
ACGCTGCTAC TGTCCGACAG GAAGGTCCCG CCCCAGCCGC TGTGGTTTGC TCCTGGATTG 
GCGAATGTCC GGAAGCTCCA GGAGTTGCCA TATCACTTGC TGCATGCCGG TCTGTGGGAG 
GAGCTACGGC AGGAAGTAGT AGGCAGCGCC GAGTGGCTCT ACTGTCAGAG CCGGGTCTGT 
GGGGTGTCCA GCCTGATCAG TGACCTGGAC CAGTGCTCCC AGTACATGGA CTGCTGTGAA 
ACTGGACTGG TCCATAAGGC TCTGGTCCTG ATGAAGCCCA GCCTGGACAT CCTGTCCGGC 
CACATGGACC GGTCTGTGTT TTACTCCGAG CTGCTGGCCC GACTCTCCTC CCTGGCCTCC 
ACCTCCCGCA CCGTGATTGG TCAGCTCTGC AGCCAGTGCG ACGATTGGCT CCAAACGTGT 
CCGGAGCCGG TTCTGATCCC GAGGAGCAGC TTCCTGCAGC AGCCAGGGGG GGCGCTGCAG 
CACACGCTGA CTGCACCACA CGGAGGTGTG CTCTGCTTGG CTGTGGGCGC GGCGGTGCGG 
CTGCTGGTCA CCGGCTCGGC CGGCGGCGTG GTGGCGGTCT GGAGCCTCCT GGACAAACAT 
CTCCTGCAGG TTCTGGAGGG ACCATCAGCG GCCATCTTGT CAGTAAAGAT CATCGAAAGC 
TCCGCCCACT GCCTCTCATT GGCTGCAGAT GGCTCCCTGA GGGGGTGGAG CTTAAAGAGT 
GGCCAGCAGC TGTTCTGCAT CCAGGAGGCG GGGCCTGCTG GCTCCGGCCC TTCCTCAGTG 
AGCCTGCAGG TGTCGGACCA GCTGCTGTTT GTTCACACCT GGATGCAGGT GAAGGTCTGG 
AAGTTGGACG GAACCGAGAT CCACAGAGAG GACATCTCGT TGGTTTTGGG GGTTCTGGGA 
GATTTTGTGG TATCTCTGCA TGACTCGGAC CGGGTCAGAA TCTTTGACCC GGTTACTGGG 
ACCCAGACGA TGGTGCGGCT GACCCGGAAC CTAACTCCAG TGGTTTCAGT GACCTCACCT 
AAACGTGGGT CATTATTTGT GGTGTCAGAC GACGGGTTCC TGCATCAGAT CTCCAGGACA 
AGACAGCAAA TCGTCACAGA GTTTCCTCTG CTTCCATCTC TTTTCTCAGC TTCAGAGGAT 
GAGAAGATCC TGATAGCAGG AAGGGAGCGG ACTCTGAGCC TGTTCCGTAT CGGCAGCAAC 
TCTGTGGACG GATTCCTGGA GCTGCAGCAC GATGATGAGG TTTTATCTGC CTGCGTGTCG 
CCGGACGGCC GACGCGTCGT CTCTGGAGCG GCGGATCAGC TGATCCGAAT CTGGTCGGTG 
ACCACCGGCC ACCTGGTGGA CACTCTGTGC GGCTGCAACG CTCCGGTCAC CGCCCTGATG 
CTCTACGACC ACTTCGTGGT CTCGGCCTCC TCGGCGGCGG ATTCGGTCCA GCTGTGGAGT 
CTGACCTACG ACCCCAGCCA CAAGCCCCTC CCACACATCC CGGCAGGCTC CGCCCACTCA 
GCCGTCACCA GAGACGGAGA TCAAGTGTTC TATGTGCGCC ACCAGACCCA GAGGGAAGTG 
CTGAGCTGGA ACAACCGCAC AGGTTCCGTG TCGGAGCGCC TGCCAGTGTC TGCGGAGGTC 
AGCTGCCTGG AGCTGGCGCA GCAGAAGCGC CTCCTGCTGT GCGGGCTGCG TAGCGGCACG 
GTGCTGATCT ACCCTCTGGC GTTGCCCCAG GAGACGCTGT GCATCCCGCC GCCAGAGGGC 
CTGGCCCCAG TGCTCTGCCT GGCCCTCGGC TCCCAGGAGA GCCTGCTGGC CGTCGCCCAC 
TGGGACACGC TGCGTCTGTT CCAGGTCGCC ACCAGAGACG GCTTCCCCGC CGTGGAGGGG 
CCCCTGGCGA CGGCCCCGCT GCTCCACGCC CCCCCGTCCC ACATGGCGCT GCTGCCCCAC 
CGCCGGCTGC TGTACGGGAC GGCCGGTGGC GAGGTGAAGC TGCACGACTT CGGCGCCGGC 
GCCGGCGCCG ACCTGCAGCC TCACAGCTCC GGGATCACCT GCGTCACGGC CAGCAACTGG 
GGGACGCACG CCCTGGTGGG CTCCCAGGAC GCCACACTGC GGCTGTGGGC CCTGGACCCG 
GTGGCCCTGG ACCACACCAT GGAGTACCAG GGGCTTTTCT TCCAGGGCGT CCTCTGCGCT 
GCCTTCGCCG ACAGCGACCG CTTCGTCTTC ACCGGATCTC AGGACCAAAC CGTCAAGGTC 
TGGGACGTCG CTTCAGGGAA GCTGCTGTGC GTCCAGTTCG TCTACTCGCC GGTCGTCCGC 
ATGCTGACCT TCAGGAATGG CTTCGTGGGC CTCACCCTGC AGGGCTGCGT CGTCCAGGAG 
GCCTTCAGGT GTCCGGACCG GGTCGACCCG GACCACAACC CTCTGAGGAA CATGAAGGCC 
CGGTACCGGG TCACCTCCAG GGAGAAGGGC GGGGCCGGCC GCTCCGACCT GCAGGGCTTC 
GACCCGGCCC AGATCAGCCT GAACCTAGTG AGCATGCTCA GCAGCAAGCC GTCCTCCGCC 
TGCGTCCTGC TGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nwd1 ( XM_007544398.2 ) cDNA ORF clone, Poecilia formosa(Amazon molly) -> XP_007544460.2 Poecilia formosa NACHT and WD repeat domain containing 1 (nwd1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OPo164608
Clone ID Related Accession (Same CDS sequence)	XM_007544398.2
Accession Version	XM_007544398.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5031bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-05-02
Organism	Poecilia formosa(Amazon molly)
Product	NACHT domain- and WD repeat-containing protein 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_006801081.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On May 3, 2016 this sequence version replaced XM_007544398.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Poecilia formosa Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGATCT TCAGAGCAGA ACCTTCAGCA GCGGCGCCGA CCAGTCCAGC TCGGTTCTGC 
AGCTTCATCA GAACCCATAA ACAGAAACAC GGAGCTCCGA GGGGAGAAAA GTGCCCAGGG 
AAACAAAGAG CTGCAGGAGC GCCGACAGAC AGAACCAGAA CCAGAACCGG CAGATGGTCT 
GAGACCATGG ATGGAGGACA AAGCTCCGGC GCGCGGGCCG TCCTGAGGGG ACGCTGCGAA 
GGAGCTCAGA GGACCGGCAG CAACATGATC CGAATCTTCA TCAGCTCCAG CTTCACAGAC 
ATGAGTATTG AGAGGAAGGT TCTGCTGGAG AAAGCGTACC CACAGGTCCG GTCTTTCTGC 
CGCAGTCTGG GTCTGGTCTT TGAGGTGGTG GATCTGAACT GGGGGATCCG GACCTTCCCA 
TATGGAGACC ATGAGGTCAG TGAGATCTTC CTGCAGGAGA TCCAGATGAG TCAGAAGGTT 
TCTGCTGGAC CGGCCTTCGT GGGCCTGCTG GGGAACCAGT ACGGTCACCG ACCTCTTCCT 
CGTCTCATCC CAGAAAAGCA GTTTGAGGTT CTTCTGTCCA CGCTGTCCAA GAATCCAGAA 
GGTGTTCAGC AACTGCAAGA GTGGTTTCGG AAGGACAACA ACTCTGTGCC ACCCACCTAT 
GTTCTCCAGC CAGTCACTGC ACGATTCCCA CACTACGATG ACCTCCGACC TGAGGTGACA 
AAGCTCCATG ACGATGACCT TCTGTCCTGG CGGGTCACGG AGAGTCAGCT GCTGCAGCTG 
CTACGCTCTG CAGCCGCTGA GGCTGAGACG GCTGGAGACA TCACAGCCGA GCAGAAGCAG 
CTCTTCTACT CATCAGTCAC AGAACGGGCG CTGGAGGAAG GCGTCTGGAA GGACAACTGC 
CAAATGTCCG CCTTGCTCTT CGTCCGGGAG ATTCCTCGGC AAAGGATGAA GGACGGATCG 
AAACGTCTGG CCAAGTTCAT GGACCTCACT GCAGACGGGC TGCTGGATGC TGAAGCTCAG 
GAGTTCCTCA CCGGCCTGAA GTCACGTCTG TATGCCACGT CCAAGAAGAT CCTCAACCTG 
CACTGCGTGG AGCTGAACAG CGGAAGCATC GACCCAAAGC GCAAGGAACA CGCCCAGTAC 
CTGGACAGCA TCTGCCAGCA GTTCATCTCC CAGATGACGA TCCGGATCAG TGAGGCTGTC 
AAACTGGCGG GGGAGGACAG GAAGAAGATC TGGGGAAGCA CAGAGGAAAA TGAGGAGGAG 
TTTGACTGGG TGGTTGAAGA GGCGAGGCAG CACACCATCA TGGCTGCTGA GTTGAGCAAT 
AGTCTCCAGG GCAGAGATGG GCTTCTGGGG AAGATCTGCC TTGCCATGTG GGAGTCCACC 
CTGGTCCACC ACGGTCCGCT GGTGGTTCAC GGCACTGCTG GGATGGGGAA GACCACTCTG 
CTGTGCAAAC TGGCACAGGA GATGCAGGAC GTCCTGGAGG CCAAGGCGGT GCTGGTTATT 
AGGCTGCTGG CTGCCAATCA TCCTCAGAGA CCAAACATCA ACCATGTCCT CAACAGCGTC 
TGCCTCCAGG TGTGTCTGGC GTGTGGCCTG GCGCCACGGC CCCTGATGTC GGTCAGCTCT 
CACCTGGAGC TGCAGCGGCT CTTCCAGAAC ATCCTGGAGA AGGTTTCTCA GCAGGGGAAC 
ACTCTGCTCC TCATCCTGGA CTCCCTGGAG CAGCTGGCAG ACCAACATCA GGCTCACCAG 
TTGCGCTGGC TGCCCACAGA CGTGCCTCCA AATGTCCACC TGCTGGTCTC CATGGAAACC 
ACCAGCGAGG CGTTTGCCAG CATGCGGCTG AGGCTGGGCA CTGCAGGAAA CGTCTTTGAG 
GTGGAGCGTT TGTCTCGTGA TGAAGGGCAG CAGATCATGG AGTCCCATCT GCAGGTGGCT 
AAGCGAACTT TGACCCGGGA GCAGAAGGAC GCGGTGCTGC AGAGCTTCCA TGCCTCCGGC 
TCTCCTCTCC ACCTTCAGCT CATCCTGTCT GCTGCCAAGA ACTGGACATC CTTCACACCA 
CCAACAGAGC TGCTGCTGGG CTCCAGCTCA GAGGAAGTGA TGTCACAGCT CCTCCTGAAG 
CTGGAGGAGA AACATGGGAA AGAGCTGGTG GGCGGGGCCT TGGGATGCAT TGTGTTGGCA 
AGGAGAGGCC TGATGGAGGC GGAACTGCGT GATGTCATGT CATTGGACGA TGATGTCATC 
AGTGAGGTAT ACAGGTATTC CCTGCCTCCA ACACCTTCAT TAATCCGGAT GCCACCTGTC 
CTGTGGGCGC GGCTCAAGCG GGACCTGCGG GACCAGCTGG AGATCCAGTG GACCGACGGA 
GTCGCCATGC TGGCCTTCAG CAGCCGGCTT CTCTCAGAGG TAGTCAAAGC CCGCTATCTG 
ACACCAGAGC GACGGGGGCG GACCCACAGG ATCCTGGCGG AGTATTTCCT GGGTCGCTGG 
TCTGGAAGAC TGAAGCCGGT GGCTCTTCCG GGACTGACGC TGCTACTGTC CGACAGGAAG 
GTCCCGCCCC AGCCGCTGTG GTTTGCTCCT GGATTGGCGA ATGTCCGGAA GCTCCAGGAG 
TTGCCATATC ACTTGCTGCA TGCCGGTCTG TGGGAGGAGC TACGGCAGGA AGTAGTAGGC 
AGCGCCGAGT GGCTCTACTG TCAGAGCCGG GTCTGTGGGG TGTCCAGCCT GATCAGTGAC 
CTGGACCAGT GCTCCCAGTA CATGGACTGC TGTGAAACTG GACTGGTCCA TAAGGCTCTG 
GTCCTGATGA AGCCCAGCCT GGACATCCTG TCCGGCCACA TGGACCGGTC TGTGTTTTAC 
TCCGAGCTGC TGGCCCGACT CTCCTCCCTG GCCTCCACCT CCCGCACCGT GATTGGTCAG 
CTCTGCAGCC AGTGCGACGA TTGGCTCCAA ACGTGTCCGG AGCCGGTTCT GATCCCGAGG 
AGCAGCTTCC TGCAGCAGCC AGGGGGGGCG CTGCAGCACA CGCTGACTGC ACCACACGGA 
GGTGTGCTCT GCTTGGCTGT GGGCGCGGCG GTGCGGCTGC TGGTCACCGG CTCGGCCGGC 
GGCGTGGTGG CGGTCTGGAG CCTCCTGGAC AAACATCTCC TGCAGGTTCT GGAGGGACCA 
TCAGCGGCCA TCTTGTCAGT AAAGATCATC GAAAGCTCCG CCCACTGCCT CTCATTGGCT 
GCAGATGGCT CCCTGAGGGG GTGGAGCTTA AAGAGTGGCC AGCAGCTGTT CTGCATCCAG 
GAGGCGGGGC CTGCTGGCTC CGGCCCTTCC TCAGTGAGCC TGCAGGTGTC GGACCAGCTG 
CTGTTTGTTC ACACCTGGAT GCAGGTGAAG GTCTGGAAGT TGGACGGAAC CGAGATCCAC 
AGAGAGGACA TCTCGTTGGT TTTGGGGGTT CTGGGAGATT TTGTGGTATC TCTGCATGAC 
TCGGACCGGG TCAGAATCTT TGACCCGGTT ACTGGGACCC AGACGATGGT GCGGCTGACC 
CGGAACCTAA CTCCAGTGGT TTCAGTGACC TCACCTAAAC GTGGGTCATT ATTTGTGGTG 
TCAGACGACG GGTTCCTGCA TCAGATCTCC AGGACAAGAC AGCAAATCGT CACAGAGTTT 
CCTCTGCTTC CATCTCTTTT CTCAGCTTCA GAGGATGAGA AGATCCTGAT AGCAGGAAGG 
GAGCGGACTC TGAGCCTGTT CCGTATCGGC AGCAACTCTG TGGACGGATT CCTGGAGCTG 
CAGCACGATG ATGAGGTTTT ATCTGCCTGC GTGTCGCCGG ACGGCCGACG CGTCGTCTCT 
GGAGCGGCGG ATCAGCTGAT CCGAATCTGG TCGGTGACCA CCGGCCACCT GGTGGACACT 
CTGTGCGGCT GCAACGCTCC GGTCACCGCC CTGATGCTCT ACGACCACTT CGTGGTCTCG 
GCCTCCTCGG CGGCGGATTC GGTCCAGCTG TGGAGTCTGA CCTACGACCC CAGCCACAAG 
CCCCTCCCAC ACATCCCGGC AGGCTCCGCC CACTCAGCCG TCACCAGAGA CGGAGATCAA 
GTGTTCTATG TGCGCCACCA GACCCAGAGG GAAGTGCTGA GCTGGAACAA CCGCACAGGT 
TCCGTGTCGG AGCGCCTGCC AGTGTCTGCG GAGGTCAGCT GCCTGGAGCT GGCGCAGCAG 
AAGCGCCTCC TGCTGTGCGG GCTGCGTAGC GGCACGGTGC TGATCTACCC TCTGGCGTTG 
CCCCAGGAGA CGCTGTGCAT CCCGCCGCCA GAGGGCCTGG CCCCAGTGCT CTGCCTGGCC 
CTCGGCTCCC AGGAGAGCCT GCTGGCCGTC GCCCACTGGG ACACGCTGCG TCTGTTCCAG 
GTCGCCACCA GAGACGGCTT CCCCGCCGTG GAGGGGCCCC TGGCGACGGC CCCGCTGCTC 
CACGCCCCCC CGTCCCACAT GGCGCTGCTG CCCCACCGCC GGCTGCTGTA CGGGACGGCC 
GGTGGCGAGG TGAAGCTGCA CGACTTCGGC GCCGGCGCCG GCGCCGACCT GCAGCCTCAC 
AGCTCCGGGA TCACCTGCGT CACGGCCAGC AACTGGGGGA CGCACGCCCT GGTGGGCTCC 
CAGGACGCCA CACTGCGGCT GTGGGCCCTG GACCCGGTGG CCCTGGACCA CACCATGGAG 
TACCAGGGGC TTTTCTTCCA GGGCGTCCTC TGCGCTGCCT TCGCCGACAG CGACCGCTTC 
GTCTTCACCG GATCTCAGGA CCAAACCGTC AAGGTCTGGG ACGTCGCTTC AGGGAAGCTG 
CTGTGCGTCC AGTTCGTCTA CTCGCCGGTC GTCCGCATGC TGACCTTCAG GAATGGCTTC 
GTGGGCCTCA CCCTGCAGGG CTGCGTCGTC CAGGAGGCCT TCAGGTGTCC GGACCGGGTC 
GACCCGGACC ACAACCCTCT GAGGAACATG AAGGCCCGGT ACCGGGTCAC CTCCAGGGAG 
AAGGGCGGGG CCGGCCGCTC CGACCTGCAG GGCTTCGACC CGGCCCAGAT CAGCCTGAAC 
CTAGTGAGCA TGCTCAGCAG CAAGCCGTCC TCCGCCTGCG TCCTGCTGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_007544460.2
CDS	112..5142
Translation	MLIFRAEPSAAAPTSPARFCSFIRTHKQKHGAPRGEKCPGKQRAAGAPTDRTRTRTGRWSETMDGGQSSGARAVLRGRCEGAQRTGSNMIRIFISSSFTDMSIERKVLLEKAYPQVRSFCRSLGLVFEVVDLNWGIRTFPYGDHEVSEIFLQEIQMSQKVSAGPAFVGLLGNQYGHRPLPRLIPEKQFEVLLSTLSKNPEGVQQLQEWFRKDNNSVPPTYVLQPVTARFPHYDDLRPEVTKLHDDDLLSWRVTESQLLQLLRSAAAEAETAGDITAEQKQLFYSSVTERALEEGVWKDNCQMSALLFVREIPRQRMKDGSKRLAKFMDLTADGLLDAEAQEFLTGLKSRLYATSKKILNLHCVELNSGSIDPKRKEHAQYLDSICQQFISQMTIRISEAVKLAGEDRKKIWGSTEENEEEFDWVVEEARQHTIMAAELSNSLQGRDGLLGKICLAMWESTLVHHGPLVVHGTAGMGKTTLLCKLAQEMQDVLEAKAVLVIRLLAANHPQRPNINHVLNSVCLQVCLACGLAPRPLMSVSSHLELQRLFQNILEKVSQQGNTLLLILDSLEQLADQHQAHQLRWLPTDVPPNVHLLVSMETTSEAFASMRLRLGTAGNVFEVERLSRDEGQQIMESHLQVAKRTLTREQKDAVLQSFHASGSPLHLQLILSAAKNWTSFTPPTELLLGSSSEEVMSQLLLKLEEKHGKELVGGALGCIVLARRGLMEAELRDVMSLDDDVISEVYRYSLPPTPSLIRMPPVLWARLKRDLRDQLEIQWTDGVAMLAFSSRLLSEVVKARYLTPERRGRTHRILAEYFLGRWSGRLKPVALPGLTLLLSDRKVPPQPLWFAPGLANVRKLQELPYHLLHAGLWEELRQEVVGSAEWLYCQSRVCGVSSLISDLDQCSQYMDCCETGLVHKALVLMKPSLDILSGHMDRSVFYSELLARLSSLASTSRTVIGQLCSQCDDWLQTCPEPVLIPRSSFLQQPGGALQHTLTAPHGGVLCLAVGAAVRLLVTGSAGGVVAVWSLLDKHLLQVLEGPSAAILSVKIIESSAHCLSLAADGSLRGWSLKSGQQLFCIQEAGPAGSGPSSVSLQVSDQLLFVHTWMQVKVWKLDGTEIHREDISLVLGVLGDFVVSLHDSDRVRIFDPVTGTQTMVRLTRNLTPVVSVTSPKRGSLFVVSDDGFLHQISRTRQQIVTEFPLLPSLFSASEDEKILIAGRERTLSLFRIGSNSVDGFLELQHDDEVLSACVSPDGRRVVSGAADQLIRIWSVTTGHLVDTLCGCNAPVTALMLYDHFVVSASSAADSVQLWSLTYDPSHKPLPHIPAGSAHSAVTRDGDQVFYVRHQTQREVLSWNNRTGSVSERLPVSAEVSCLELAQQKRLLLCGLRSGTVLIYPLALPQETLCIPPPEGLAPVLCLALGSQESLLAVAHWDTLRLFQVATRDGFPAVEGPLATAPLLHAPPSHMALLPHRRLLYGTAGGEVKLHDFGAGAGADLQPHSSGITCVTASNWGTHALVGSQDATLRLWALDPVALDHTMEYQGLFFQGVLCAAFADSDRFVFTGSQDQTVKVWDVASGKLLCVQFVYSPVVRMLTFRNGFVGLTLQGCVVQEAFRCPDRVDPDHNPLRNMKARYRVTSREKGGAGRSDLQGFDPAQISLNLVSMLSSKPSSACVLL

Target ORF information:

RefSeq Version	XM_007544398.2
Organism	Poecilia formosa(Amazon molly)
Definition	Poecilia formosa NACHT and WD repeat domain containing 1 (nwd1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_007544398.2

ORF Insert Sequence:

ATGCTGATCT TCAGAGCAGA ACCTTCAGCA GCGGCGCCGA CCAGTCCAGC TCGGTTCTGC 
AGCTTCATCA GAACCCATAA ACAGAAACAC GGAGCTCCGA GGGGAGAAAA GTGCCCAGGG 
AAACAAAGAG CTGCAGGAGC GCCGACAGAC AGAACCAGAA CCAGAACCGG CAGATGGTCT 
GAGACCATGG ATGGAGGACA AAGCTCCGGC GCGCGGGCCG TCCTGAGGGG ACGCTGCGAA 
GGAGCTCAGA GGACCGGCAG CAACATGATC CGAATCTTCA TCAGCTCCAG CTTCACAGAC 
ATGAGTATTG AGAGGAAGGT TCTGCTGGAG AAAGCGTACC CACAGGTCCG GTCTTTCTGC 
CGCAGTCTGG GTCTGGTCTT TGAGGTGGTG GATCTGAACT GGGGGATCCG GACCTTCCCA 
TATGGAGACC ATGAGGTCAG TGAGATCTTC CTGCAGGAGA TCCAGATGAG TCAGAAGGTT 
TCTGCTGGAC CGGCCTTCGT GGGCCTGCTG GGGAACCAGT ACGGTCACCG ACCTCTTCCT 
CGTCTCATCC CAGAAAAGCA GTTTGAGGTT CTTCTGTCCA CGCTGTCCAA GAATCCAGAA 
GGTGTTCAGC AACTGCAAGA GTGGTTTCGG AAGGACAACA ACTCTGTGCC ACCCACCTAT 
GTTCTCCAGC CAGTCACTGC ACGATTCCCA CACTACGATG ACCTCCGACC TGAGGTGACA 
AAGCTCCATG ACGATGACCT TCTGTCCTGG CGGGTCACGG AGAGTCAGCT GCTGCAGCTG 
CTACGCTCTG CAGCCGCTGA GGCTGAGACG GCTGGAGACA TCACAGCCGA GCAGAAGCAG 
CTCTTCTACT CATCAGTCAC AGAACGGGCG CTGGAGGAAG GCGTCTGGAA GGACAACTGC 
CAAATGTCCG CCTTGCTCTT CGTCCGGGAG ATTCCTCGGC AAAGGATGAA GGACGGATCG 
AAACGTCTGG CCAAGTTCAT GGACCTCACT GCAGACGGGC TGCTGGATGC TGAAGCTCAG 
GAGTTCCTCA CCGGCCTGAA GTCACGTCTG TATGCCACGT CCAAGAAGAT CCTCAACCTG 
CACTGCGTGG AGCTGAACAG CGGAAGCATC GACCCAAAGC GCAAGGAACA CGCCCAGTAC 
CTGGACAGCA TCTGCCAGCA GTTCATCTCC CAGATGACGA TCCGGATCAG TGAGGCTGTC 
AAACTGGCGG GGGAGGACAG GAAGAAGATC TGGGGAAGCA CAGAGGAAAA TGAGGAGGAG 
TTTGACTGGG TGGTTGAAGA GGCGAGGCAG CACACCATCA TGGCTGCTGA GTTGAGCAAT 
AGTCTCCAGG GCAGAGATGG GCTTCTGGGG AAGATCTGCC TTGCCATGTG GGAGTCCACC 
CTGGTCCACC ACGGTCCGCT GGTGGTTCAC GGCACTGCTG GGATGGGGAA GACCACTCTG 
CTGTGCAAAC TGGCACAGGA GATGCAGGAC GTCCTGGAGG CCAAGGCGGT GCTGGTTATT 
AGGCTGCTGG CTGCCAATCA TCCTCAGAGA CCAAACATCA ACCATGTCCT CAACAGCGTC 
TGCCTCCAGG TGTGTCTGGC GTGTGGCCTG GCGCCACGGC CCCTGATGTC GGTCAGCTCT 
CACCTGGAGC TGCAGCGGCT CTTCCAGAAC ATCCTGGAGA AGGTTTCTCA GCAGGGGAAC 
ACTCTGCTCC TCATCCTGGA CTCCCTGGAG CAGCTGGCAG ACCAACATCA GGCTCACCAG 
TTGCGCTGGC TGCCCACAGA CGTGCCTCCA AATGTCCACC TGCTGGTCTC CATGGAAACC 
ACCAGCGAGG CGTTTGCCAG CATGCGGCTG AGGCTGGGCA CTGCAGGAAA CGTCTTTGAG 
GTGGAGCGTT TGTCTCGTGA TGAAGGGCAG CAGATCATGG AGTCCCATCT GCAGGTGGCT 
AAGCGAACTT TGACCCGGGA GCAGAAGGAC GCGGTGCTGC AGAGCTTCCA TGCCTCCGGC 
TCTCCTCTCC ACCTTCAGCT CATCCTGTCT GCTGCCAAGA ACTGGACATC CTTCACACCA 
CCAACAGAGC TGCTGCTGGG CTCCAGCTCA GAGGAAGTGA TGTCACAGCT CCTCCTGAAG 
CTGGAGGAGA AACATGGGAA AGAGCTGGTG GGCGGGGCCT TGGGATGCAT TGTGTTGGCA 
AGGAGAGGCC TGATGGAGGC GGAACTGCGT GATGTCATGT CATTGGACGA TGATGTCATC 
AGTGAGGTAT ACAGGTATTC CCTGCCTCCA ACACCTTCAT TAATCCGGAT GCCACCTGTC 
CTGTGGGCGC GGCTCAAGCG GGACCTGCGG GACCAGCTGG AGATCCAGTG GACCGACGGA 
GTCGCCATGC TGGCCTTCAG CAGCCGGCTT CTCTCAGAGG TAGTCAAAGC CCGCTATCTG 
ACACCAGAGC GACGGGGGCG GACCCACAGG ATCCTGGCGG AGTATTTCCT GGGTCGCTGG 
TCTGGAAGAC TGAAGCCGGT GGCTCTTCCG GGACTGACGC TGCTACTGTC CGACAGGAAG 
GTCCCGCCCC AGCCGCTGTG GTTTGCTCCT GGATTGGCGA ATGTCCGGAA GCTCCAGGAG 
TTGCCATATC ACTTGCTGCA TGCCGGTCTG TGGGAGGAGC TACGGCAGGA AGTAGTAGGC 
AGCGCCGAGT GGCTCTACTG TCAGAGCCGG GTCTGTGGGG TGTCCAGCCT GATCAGTGAC 
CTGGACCAGT GCTCCCAGTA CATGGACTGC TGTGAAACTG GACTGGTCCA TAAGGCTCTG 
GTCCTGATGA AGCCCAGCCT GGACATCCTG TCCGGCCACA TGGACCGGTC TGTGTTTTAC 
TCCGAGCTGC TGGCCCGACT CTCCTCCCTG GCCTCCACCT CCCGCACCGT GATTGGTCAG 
CTCTGCAGCC AGTGCGACGA TTGGCTCCAA ACGTGTCCGG AGCCGGTTCT GATCCCGAGG 
AGCAGCTTCC TGCAGCAGCC AGGGGGGGCG CTGCAGCACA CGCTGACTGC ACCACACGGA 
GGTGTGCTCT GCTTGGCTGT GGGCGCGGCG GTGCGGCTGC TGGTCACCGG CTCGGCCGGC 
GGCGTGGTGG CGGTCTGGAG CCTCCTGGAC AAACATCTCC TGCAGGTTCT GGAGGGACCA 
TCAGCGGCCA TCTTGTCAGT AAAGATCATC GAAAGCTCCG CCCACTGCCT CTCATTGGCT 
GCAGATGGCT CCCTGAGGGG GTGGAGCTTA AAGAGTGGCC AGCAGCTGTT CTGCATCCAG 
GAGGCGGGGC CTGCTGGCTC CGGCCCTTCC TCAGTGAGCC TGCAGGTGTC GGACCAGCTG 
CTGTTTGTTC ACACCTGGAT GCAGGTGAAG GTCTGGAAGT TGGACGGAAC CGAGATCCAC 
AGAGAGGACA TCTCGTTGGT TTTGGGGGTT CTGGGAGATT TTGTGGTATC TCTGCATGAC 
TCGGACCGGG TCAGAATCTT TGACCCGGTT ACTGGGACCC AGACGATGGT GCGGCTGACC 
CGGAACCTAA CTCCAGTGGT TTCAGTGACC TCACCTAAAC GTGGGTCATT ATTTGTGGTG 
TCAGACGACG GGTTCCTGCA TCAGATCTCC AGGACAAGAC AGCAAATCGT CACAGAGTTT 
CCTCTGCTTC CATCTCTTTT CTCAGCTTCA GAGGATGAGA AGATCCTGAT AGCAGGAAGG 
GAGCGGACTC TGAGCCTGTT CCGTATCGGC AGCAACTCTG TGGACGGATT CCTGGAGCTG 
CAGCACGATG ATGAGGTTTT ATCTGCCTGC GTGTCGCCGG ACGGCCGACG CGTCGTCTCT 
GGAGCGGCGG ATCAGCTGAT CCGAATCTGG TCGGTGACCA CCGGCCACCT GGTGGACACT 
CTGTGCGGCT GCAACGCTCC GGTCACCGCC CTGATGCTCT ACGACCACTT CGTGGTCTCG 
GCCTCCTCGG CGGCGGATTC GGTCCAGCTG TGGAGTCTGA CCTACGACCC CAGCCACAAG 
CCCCTCCCAC ACATCCCGGC AGGCTCCGCC CACTCAGCCG TCACCAGAGA CGGAGATCAA 
GTGTTCTATG TGCGCCACCA GACCCAGAGG GAAGTGCTGA GCTGGAACAA CCGCACAGGT 
TCCGTGTCGG AGCGCCTGCC AGTGTCTGCG GAGGTCAGCT GCCTGGAGCT GGCGCAGCAG 
AAGCGCCTCC TGCTGTGCGG GCTGCGTAGC GGCACGGTGC TGATCTACCC TCTGGCGTTG 
CCCCAGGAGA CGCTGTGCAT CCCGCCGCCA GAGGGCCTGG CCCCAGTGCT CTGCCTGGCC 
CTCGGCTCCC AGGAGAGCCT GCTGGCCGTC GCCCACTGGG ACACGCTGCG TCTGTTCCAG 
GTCGCCACCA GAGACGGCTT CCCCGCCGTG GAGGGGCCCC TGGCGACGGC CCCGCTGCTC 
CACGCCCCCC CGTCCCACAT GGCGCTGCTG CCCCACCGCC GGCTGCTGTA CGGGACGGCC 
GGTGGCGAGG TGAAGCTGCA CGACTTCGGC GCCGGCGCCG GCGCCGACCT GCAGCCTCAC 
AGCTCCGGGA TCACCTGCGT CACGGCCAGC AACTGGGGGA CGCACGCCCT GGTGGGCTCC 
CAGGACGCCA CACTGCGGCT GTGGGCCCTG GACCCGGTGG CCCTGGACCA CACCATGGAG 
TACCAGGGGC TTTTCTTCCA GGGCGTCCTC TGCGCTGCCT TCGCCGACAG CGACCGCTTC 
GTCTTCACCG GATCTCAGGA CCAAACCGTC AAGGTCTGGG ACGTCGCTTC AGGGAAGCTG 
CTGTGCGTCC AGTTCGTCTA CTCGCCGGTC GTCCGCATGC TGACCTTCAG GAATGGCTTC 
GTGGGCCTCA CCCTGCAGGG CTGCGTCGTC CAGGAGGCCT TCAGGTGTCC GGACCGGGTC 
GACCCGGACC ACAACCCTCT GAGGAACATG AAGGCCCGGT ACCGGGTCAC CTCCAGGGAG 
AAGGGCGGGG CCGGCCGCTC CGACCTGCAG GGCTTCGACC CGGCCCAGAT CAGCCTGAAC 
CTAGTGAGCA TGCTCAGCAG CAAGCCGTCC TCCGCCTGCG TCCTGCTGTA G