ddx59 cDNA ORF clone, Poecilia formosa(Amazon molly)

Gene
Clones
gRNAs

Summary

Gene Symbol	ddx59
Entrez Gene ID	103130117
Full Name	DEAD-box helicase 59
Gene Type	protein-coding
Organism	Poecilia formosa(Amazon molly)

mRNA and Protein(s)

mRNA	Protein	Name
XM_007541436.2	XP_007541498.1	probable ATP-dependent RNA helicase DDX59

Pathways from BioSystems
Gene Ontology

The following ddx59 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ddx59 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OPo129494	XM_007541436.2 Latest version!	Poecilia formosa DEAD-box helicase 59 (ddx59), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ddx59 ( XM_007541436.2 ) cDNA ORF clone, Poecilia formosa(Amazon molly) -> XP_007541498.1 Poecilia formosa DEAD-box helicase 59 (ddx59), mRNA.

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CloneID	OPo129494
Clone ID Related Accession (Same CDS sequence)	XM_007541436.2
Accession Version	XM_007541436.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1872bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-05-02
Organism	Poecilia formosa(Amazon molly)
Product	probable ATP-dependent RNA helicase DDX59
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_006799949.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On May 3, 2016 this sequence version replaced XM_007541436.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Poecilia formosa Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTTATGC CAAGAGCACT GAAAGTAAAG AGACCTGTCC AGGCACCAGG GCACGGATTA 
AATAAGAAAA GCAAGACTGA TCAAGAGGAT TGTGACCAAA TAAACTTGCA GGCATCCACG 
CAGAAAGATG AAGCATGGGA AGACTTAAAA ACACAGACTG ACAAAGGTGA ACATTTAACG 
GAGACCGCAA AATGTGAATC TGCAGCTCAG TCCTCCTCAG ATGACGCAGA AGAAGAAGAA 
CCAGTGAAAT CTTTCAAAAA GAGCCAGAGA TGGCCAGAGC CAGGAGAGCC TGTCTGTGTG 
ATGTGCGGAC GTTATGGGGA GTACATATGT GATGCTACTG ATAATGATGT TTGCAGCCTT 
GAATGCAAAG CCAATCATTT ACTGCAAATG GGAATTAAGC CTGGACATGA TGCCTTTAAT 
GTTAAGGATA AAAACACAGA TGGAAGGACT TCTCAACCTC ATCATGTAGC TGTAAATCTG 
GGTACAAGTG AAGCAGGCTA TTCCTACAGG GAAGATCCGT TCATCTCAGG CCTCACAGAT 
GAACAAGTGG AGCGAATTAG ATTGGAGCTT GGCATTAAAA CCGAGGGAAA AGATGTCCGA 
AGACCTATTG TTGAGTTTGA ACACTGTGGT TTTCCATCCT TGTTAAGTGG CAACCTGAAA 
AAAGCTGGTT ATGAGGCTCC AACGCCAGTC CAGATGCAGA TGGTGCCTGT TGGCCTCACA 
GGGAGGGATG TGATAGCCAG CGCTGACACA GGATCAGGGA AGACCATAGC TTTCCTGCTG 
CCTGTGGTTG TGAGAGCACT AGAGGAAGCA GCTCACGGCG TATGCAGTCC ATTGGGTCTG 
ATTCTGACCC CCACCAGAGA GCTGGCCATA CAGATAGAGA GACAGGCCAA GGAGCTGGTG 
ATGGGCATCC CTAACATGAG AACAGCCCTG CTTGTGGGAG GCATGCCTCT TCCCCCACAG 
CTGCACCGCC TCAAGAGCAG CATCAAAATT GTTATCGCTA CGCCTGGGAG GCTCATTGAA 
ATCCTGAAAC AAAAAGCACT TAAACTTGAC AAAGTAAAGA TTTTGGTGGT TGATGAGGTT 
GACACCATGT TGAAGATGGG CTTCCAGCAA CAAGTTCTGG AGGTTCTGGA GCATGTTACA 
GAGGAACACC AGACTCTACT GGCTTCGGCC ACTATCCCAG CAGGGACGGA GGACTTGGCT 
GCTCGCTTGG TCCATGACCC TGTGCGCATT GCTATCGGTG AGAAGAACCA ACCGTGTGCT 
AATGTCAGGC AGATCCTACT TTGGGTGGAG GAGCCCTCTA AGAAGAAGAA GCTGTTTGAA 
ATACTTAATG ATAACAAACT GTTCCAGCCT CCAGTCGTGG TGTTTGTCGA CTGTAAACTG 
GGTGCCGATC TCCTGTGTGA GGCAGTTGCC AAGGTGACGG GCCTAACTAC TGTAGCAATT 
CACTCTGACA AAAACCAGAG GGAGCGCAAC CGCATCCTTA AGGGTCTTCT GGATGGAGAC 
TTTGAAGTAG TAATCAGTAC AGGTGTGCTG GGCAGAGGAC TAGACCTTGT CAATGTGAGA 
TTAGTGGTTA ACTTTGACAT GCCAAACACA ATGGATGAGT ATGTGCATCA GGTGGGGAGG 
GCGGGAAGGC TGGGCCACAG GGGAACAGCG ATCACCTTCC TGAACAACAA CAACAAACGG 
CTGTTCCTGG AGGTGGTGAA CAGGGTCAAA CCCACTGGGT CCATCCTGCC CCCTCAGCTC 
CTAAACTCAC CATACCTCTT TGAACAGCAG AAGAGGGAAA GTCAGAAGAA CAAGATGCAA 
CATGACGACA TACTGGTTAC TAAAAACAAC CTCTTGGACA TCATAAAGAA ACATGACCGT 
CGCAAGAAGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_007541498.1
CDS	112..1983
Translation	MFMPRALKVKRPVQAPGHGLNKKSKTDQEDCDQINLQASTQKDEAWEDLKTQTDKGEHLTETAKCESAAQSSSDDAEEEEPVKSFKKSQRWPEPGEPVCVMCGRYGEYICDATDNDVCSLECKANHLLQMGIKPGHDAFNVKDKNTDGRTSQPHHVAVNLGTSEAGYSYREDPFISGLTDEQVERIRLELGIKTEGKDVRRPIVEFEHCGFPSLLSGNLKKAGYEAPTPVQMQMVPVGLTGRDVIASADTGSGKTIAFLLPVVVRALEEAAHGVCSPLGLILTPTRELAIQIERQAKELVMGIPNMRTALLVGGMPLPPQLHRLKSSIKIVIATPGRLIEILKQKALKLDKVKILVVDEVDTMLKMGFQQQVLEVLEHVTEEHQTLLASATIPAGTEDLAARLVHDPVRIAIGEKNQPCANVRQILLWVEEPSKKKKLFEILNDNKLFQPPVVVFVDCKLGADLLCEAVAKVTGLTTVAIHSDKNQRERNRILKGLLDGDFEVVISTGVLGRGLDLVNVRLVVNFDMPNTMDEYVHQVGRAGRLGHRGTAITFLNNNNKRLFLEVVNRVKPTGSILPPQLLNSPYLFEQQKRESQKNKMQHDDILVTKNNLLDIIKKHDRRKK

Target ORF information:

RefSeq Version	XM_007541436.2
Organism	Poecilia formosa(Amazon molly)
Definition	Poecilia formosa DEAD-box helicase 59 (ddx59), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_007541436.2

ORF Insert Sequence:

ATGTTTATGC CAAGAGCACT GAAAGTAAAG AGACCTGTCC AGGCACCAGG GCACGGATTA 
AATAAGAAAA GCAAGACTGA TCAAGAGGAT TGTGACCAAA TAAACTTGCA GGCATCCACG 
CAGAAAGATG AAGCATGGGA AGACTTAAAA ACACAGACTG ACAAAGGTGA ACATTTAACG 
GAGACCGCAA AATGTGAATC TGCAGCTCAG TCCTCCTCAG ATGACGCAGA AGAAGAAGAA 
CCAGTGAAAT CTTTCAAAAA GAGCCAGAGA TGGCCAGAGC CAGGAGAGCC TGTCTGTGTG 
ATGTGCGGAC GTTATGGGGA GTACATATGT GATGCTACTG ATAATGATGT TTGCAGCCTT 
GAATGCAAAG CCAATCATTT ACTGCAAATG GGAATTAAGC CTGGACATGA TGCCTTTAAT 
GTTAAGGATA AAAACACAGA TGGAAGGACT TCTCAACCTC ATCATGTAGC TGTAAATCTG 
GGTACAAGTG AAGCAGGCTA TTCCTACAGG GAAGATCCGT TCATCTCAGG CCTCACAGAT 
GAACAAGTGG AGCGAATTAG ATTGGAGCTT GGCATTAAAA CCGAGGGAAA AGATGTCCGA 
AGACCTATTG TTGAGTTTGA ACACTGTGGT TTTCCATCCT TGTTAAGTGG CAACCTGAAA 
AAAGCTGGTT ATGAGGCTCC AACGCCAGTC CAGATGCAGA TGGTGCCTGT TGGCCTCACA 
GGGAGGGATG TGATAGCCAG CGCTGACACA GGATCAGGGA AGACCATAGC TTTCCTGCTG 
CCTGTGGTTG TGAGAGCACT AGAGGAAGCA GCTCACGGCG TATGCAGTCC ATTGGGTCTG 
ATTCTGACCC CCACCAGAGA GCTGGCCATA CAGATAGAGA GACAGGCCAA GGAGCTGGTG 
ATGGGCATCC CTAACATGAG AACAGCCCTG CTTGTGGGAG GCATGCCTCT TCCCCCACAG 
CTGCACCGCC TCAAGAGCAG CATCAAAATT GTTATCGCTA CGCCTGGGAG GCTCATTGAA 
ATCCTGAAAC AAAAAGCACT TAAACTTGAC AAAGTAAAGA TTTTGGTGGT TGATGAGGTT 
GACACCATGT TGAAGATGGG CTTCCAGCAA CAAGTTCTGG AGGTTCTGGA GCATGTTACA 
GAGGAACACC AGACTCTACT GGCTTCGGCC ACTATCCCAG CAGGGACGGA GGACTTGGCT 
GCTCGCTTGG TCCATGACCC TGTGCGCATT GCTATCGGTG AGAAGAACCA ACCGTGTGCT 
AATGTCAGGC AGATCCTACT TTGGGTGGAG GAGCCCTCTA AGAAGAAGAA GCTGTTTGAA 
ATACTTAATG ATAACAAACT GTTCCAGCCT CCAGTCGTGG TGTTTGTCGA CTGTAAACTG 
GGTGCCGATC TCCTGTGTGA GGCAGTTGCC AAGGTGACGG GCCTAACTAC TGTAGCAATT 
CACTCTGACA AAAACCAGAG GGAGCGCAAC CGCATCCTTA AGGGTCTTCT GGATGGAGAC 
TTTGAAGTAG TAATCAGTAC AGGTGTGCTG GGCAGAGGAC TAGACCTTGT CAATGTGAGA 
TTAGTGGTTA ACTTTGACAT GCCAAACACA ATGGATGAGT ATGTGCATCA GGTGGGGAGG 
GCGGGAAGGC TGGGCCACAG GGGAACAGCG ATCACCTTCC TGAACAACAA CAACAAACGG 
CTGTTCCTGG AGGTGGTGAA CAGGGTCAAA CCCACTGGGT CCATCCTGCC CCCTCAGCTC 
CTAAACTCAC CATACCTCTT TGAACAGCAG AAGAGGGAAA GTCAGAAGAA CAAGATGCAA 
CATGACGACA TACTGGTTAC TAAAAACAAC CTCTTGGACA TCATAAAGAA ACATGACCGT 
CGCAAGAAGT AA