UBE4B cDNA ORF clone, Phascolarctos cinereus(koala)

Gene
Clones
gRNAs

Summary

Gene Symbol	UBE4B
Entrez Gene ID	110199376
Full Name	ubiquitination factor E4B
Gene Type	protein-coding
Organism	Phascolarctos cinereus(koala)

mRNA and Protein(s)

mRNA	Protein	Name
XM_020974185.1	XP_020829844.1	ubiquitin conjugation factor E4 B isoform X2
XM_020974176.1	XP_020829835.1	ubiquitin conjugation factor E4 B isoform X1

Pathways from BioSystems
Gene Ontology

The following UBE4B gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the UBE4B cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OPh250262	XM_020974176.1 Latest version!	Phascolarctos cinereus ubiquitination factor E4B (UBE4B), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
OPh250263	XM_020974185.1 Latest version!	Phascolarctos cinereus ubiquitination factor E4B (UBE4B), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

UBE4B ( XM_020974176.1 ) cDNA ORF clone, Phascolarctos cinereus(koala) -> XP_020829835.1 Phascolarctos cinereus ubiquitination factor E4B (UBE4B), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OPh250262
Clone ID Related Accession (Same CDS sequence)	XM_020974176.1
Accession Version	XM_020974176.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4065bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-05-09
Organism	Phascolarctos cinereus(koala)
Product	ubiquitin conjugation factor E4 B isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018343960.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Phascolarctos cinereus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGAGC TGAGCGCTGA TGAGATCCGA CGGAGGCGCC TTGCTCGACT TGCTGGTGGA 
CAGACTTCAC AGCCCTCTAC CCCACTTACC TCTCCACAGA GAGAAAACCC ACCAGGCCCT 
CCTGTAGCAG CACCAGCCCC AGGCCCTTCC CATAATCTCG GTCTAAATGT CCACAACATG 
ACCCCAGCTA CCTCCCCGAT AGGTGCATCA GGAGTCACGC ATCGAAGCCA GAGCAGTGAA 
GGAGTCAGTT CACTCAGCAG CTCACCGTCT AATAGCCTTG AAACACAGTC ACAGTCTCTC 
TCACGGTCAC AGAGCATGGA TATCGACAGT GTCTCCTGCG AGAAAAGCAT GTCCCAGGTG 
GATGTAGATT CAGGAATTGA AAATATGGAG GTTGATGAAA ATGATCGCAG AGAAAAAAGG 
ACCCTCTCTG ATAAGGAACT TTCCTCAGGT TCTGAGGTAT CAGAAGAACA GGCCTTGCAG 
CTGGTCTGTA AGATCTTTCG TGTCTCTTGG AAAGACCGGG ACAGAGACGT CATCTTTTTG 
ACTTCACTTT CTGCACAGTT TAAGCAGAAC CCAAAAGAAG TGTTCTCTGA TTTTAAGGAT 
TTGATTGGCC AGATTTTAAT GGAAGTGCTA ATGATGTCCA CTCAAACCAG AGATGAAAAT 
CCTTTTGCCA GTCTGACGGC GACGTCCCAG CCGATTGCAA CCGCAGCTCG GTCGCCAGAC 
AGAAACCTGA TGCTTAGTAC GGGATCCAAT CCAGGAACGA GCCCCATGTT CTGTAACGTG 
GGTTCCTTCA GTGCCAGCTC GCTGTCCAGC CTCTATGACA CTAGTCCAAC TCCCAATACC 
CCTTTCTCAA GCTATGTGCC AGTGACGAGC TCCTCCTGTG CCTCAGCTTC CGATGCAGCC 
GCTCCAATTT CTGTGCCTTC CATTTCGCTC AGTCCTCATA TTCCAGGAGA AAGCCCTTCA 
GGAAGCCAGG CTTCGTCTCC GAGGTATCGC CCTTACACTG TGGCCCCACC CTGGGGCTTT 
TCGACTTCTC CCACCCCGAG GTCCTCGAGC CTGTCAGCTT TATCTAGCTC ACCAAGCCCG 
CCTGCCCTGT CGACCAGCCC TGCCAGACAA AGACCCACAG CCATGGGCCC ACCCTTGTTT 
CCTGCTTCTC CAGGTCCCGT GAGCAGGCGT CCCTCTGCCC TAAGGATTCC TTCTAGTCCG 
TACGACCATC CTTTCTCCTT CCTCTTCCTC GCGCTTTCTG ATATGTCTGG GGACAGTAGT 
GATGAAGAAA ATGAAGATGG TGATGGTGAT GATGAGGATG AGGATGAAGG TGGTGGTGAT 
GATGATTTTG CTTGTGTCCA GTTTGGGTCC AGTTTGGGAG CCTCTGGTGG GGCAAGTGCT 
TCTGATTCCT GCAGTGACCA TTTCACCATT GAAACATGCA AGGAGACAGA TATGCTGAAC 
TACCTCATCG AATGTTTTGA CAGAGTTGGA ATAGAGGAAA GAAAAGCACC AAAGATGTGC 
AGCCAGCCGG CGGTCAGCCA GTTACTGAGT AACATCCGCT CACAGTGCAT CTCCCACGCT 
GCGTTAGTGT TACAGGGCTC ATTAACGCAG CCAAGGTCCA TGCAGCAGCA GTCTCTCTTG 
GTACCCTATA TGCTGTGTAG GAACCTCCCA TTTGGTTTCA TCCAAGAGCT GGTGAGAACA 
ACCCACCAGG ATGAAGAAGT GTTCAAACAG ATCTTTATCC CTATTTTACA AGGTTTGGCT 
CTTGCTGCCA AAGACTGCTC CCTTGATAGT GACTACTTTA AATACCCCCT TATGGCATTA 
GGTGAACTCT GTGAAATTAA GTTTGGGAAA ACCCATCCCA TTTGTAGTTT GGTCGCCTCT 
TTGCCTTTGT GGCTGCCGAA GTCCTTAAGC CCAGGTTCTG GGCGGGAGTT GCAAAGACTC 
TCTTATTTGG GGGCCTTCTT CAGTCTCTCC GTCTTTGCCG AGGATGATGC AAAGGTGGTT 
GAAAAGTATT TCTCAGGGCC TGCCATTACC CTAGAGAACA CCCGAGTGGT CAGCCAGTCA 
CTACAGCATT ACTTGGAGTC AGCCAGACAA GAATTGTTCA AGATTCTACA TAGTATTTTG 
CTGAATGGTG AAACTCGTGA GGCAGCTCTC AGTTACATGG CAGCTATCGT CAATGCCAAC 
ATGAAGAAAG CGCAGATGCA GACAGATGAT CGGTTGGTAT CTACGGATGG ATTTATGCTG 
AATTTCCTTT GGGTGCTGCA GCAGTTAAGC ACAAAGATCA AGCTGGAGAC GATAGATCCC 
ACCTATATAT TCCATCCGCG GTGTCGTATT GTTCTTCCGA GCGACGAGAC CCGAGTGAAG 
GCAACGATGG AGGAGGTTAC TGGTTGGATG ACTGAACTTT ATGGAGATCA GTCTCCATTT 
TCTGAGCCGA AATTCCCTAC AGAATGCTTT TTCCTTACGC TGCATGCCCA CCACCTCTCT 
ATTTTGCCAA GCTGTCGTCG TTATATACGC AGACTCCGGG CCATCCGGGA GCTTAACAGA 
ACTGTAGAAG ATCTTAAAAA CAACGAAAGT CAGTGGAAAG ATTCCCCACT GGCAACTAGA 
CATCGGGAAA TGTTGAAACG TTGCAAAACT CAACTCAAGA AATTGGTGCG ATGTAAAGCC 
TGTGCTGATG CAGGCTTGCT GGACGAAAAC TTCCTGCGGA GATGTCTGAA TTTCTATGGC 
ATCGTTATCC AGCTGCTGCT GCGTATCCTT GACCCTGCCT ATCCTCATAT CACACTGCCT 
TTAAATCCAG ATGTCCCTAA GGTGTTTGCA GCATTGCCTG AGTTTTACGT GGAAGACGTT 
GCTGAATTTT TATTTTTTAT TGTACAATAC TCCCCTCAGG TGCTGTATGA GCCCTGTACT 
CAGGATATTG CCATGTTCCT TGTTGTGATG CTGTGCAACC AGAACTATAT CCGGAATCCA 
TACCTGGTGG CCAAATTGGT GGAGGTCATG TTCATGACCA ACCCTGCTGT CCAGCCGCGG 
ACCCAGAAGT TTTTTGAAAT GATAGAGAAC CACCCTCTTT CCACCAAGCT GCTAGTCCCC 
TCACTGATGA AGTTTTATAC AGATGTTGAA CACACTGGAG CCACCAGCGA GTTCTATGAC 
AAATTTACAA TCCGATATCA TATCAGCACC ATTTTTAAAA GTCTTTGGCA AAACATAGCC 
CATCATGGCA CCTTTATGGA AGAGTTCAAT TCTGGGAAGC AGTTTGTTCG GTACATAAAC 
ATGCTGATAA ATGACACAAC GTTTTTGTTG GATGAGAGTC TGGAGTCCTT GAAGCGGATC 
CATGAAGTGC AAGAAGAAAT GAAGAACAAG GAACAGTGGG ACCTGTTGCC CCGGGACCAG 
CAGCAGGCCC GTCAGTCTCA GCTCGCTCAG GACGAGCGTG TGTCTCGCTC GTATCTTGCA 
CTAGCAACAG AAACGGTGGA TATGTTTCAT ATTCTCACCA AGCAAGTCCA GAAGCCTTTC 
CTCAGACCTG AACTTGGACC ACGCTTGGCT GCTATGCTGA ATTTTAATCT TCAGCAACTC 
TGTGGCCCCA AGTGCCGCGA CCTGAAAGTG GAAAACCCTG AGAAATATGG GTTTGAACCA 
AAGAAGCTCT TGGACCAATT GACAGATATT TACTTGCAGC TAGACTGTGC CCGCTTTGCT 
AAGGCCATTG CGGATGACCA GAGATCATAT AGCAAGGAGC TATTTGAAGA GGTGATTTTG 
AAGATGCGCA AAGCAGGAAT TAAGTCCACA ATAGCAATAG AGAAGTTTAA GCTGCTGGCA 
GAGAAGGTGG AGGAAATAGT GGCCAAGAAT GCTCGGGCCG AAATTGATTA CAGTGATGCT 
CCAGATGAAT TCAGAGACCC ATTAATGGAC ACCCTAATGA CTGACCCTGT GAGGTTGCCC 
TCTGGCACCA TCATGGACCG CTCAATCATT CTTCGGCACC TGCTGAATTC ACCCACTGAT 
CCCTTCAATC GACAGATGCT GACGGAGAGC ATGCTGGAGC CAGTGCCAGA ATTGAAAGAA 
CAAATCCAAG CCTGGATGAG AGAGAAACAG AACACTGATC ATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_020829835.1
CDS	503..4567
Translation	MEELSADEIRRRRLARLAGGQTSQPSTPLTSPQRENPPGPPVAAPAPGPSHNLGLNVHNMTPATSPIGASGVTHRSQSSEGVSSLSSSPSNSLETQSQSLSRSQSMDIDSVSCEKSMSQVDVDSGIENMEVDENDRREKRTLSDKELSSGSEVSEEQALQLVCKIFRVSWKDRDRDVIFLTSLSAQFKQNPKEVFSDFKDLIGQILMEVLMMSTQTRDENPFASLTATSQPIATAARSPDRNLMLSTGSNPGTSPMFCNVGSFSASSLSSLYDTSPTPNTPFSSYVPVTSSSCASASDAAAPISVPSISLSPHIPGESPSGSQASSPRYRPYTVAPPWGFSTSPTPRSSSLSALSSSPSPPALSTSPARQRPTAMGPPLFPASPGPVSRRPSALRIPSSPYDHPFSFLFLALSDMSGDSSDEENEDGDGDDEDEDEGGGDDDFACVQFGSSLGASGGASASDSCSDHFTIETCKETDMLNYLIECFDRVGIEERKAPKMCSQPAVSQLLSNIRSQCISHAALVLQGSLTQPRSMQQQSLLVPYMLCRNLPFGFIQELVRTTHQDEEVFKQIFIPILQGLALAAKDCSLDSDYFKYPLMALGELCEIKFGKTHPICSLVASLPLWLPKSLSPGSGRELQRLSYLGAFFSLSVFAEDDAKVVEKYFSGPAITLENTRVVSQSLQHYLESARQELFKILHSILLNGETREAALSYMAAIVNANMKKAQMQTDDRLVSTDGFMLNFLWVLQQLSTKIKLETIDPTYIFHPRCRIVLPSDETRVKATMEEVTGWMTELYGDQSPFSEPKFPTECFFLTLHAHHLSILPSCRRYIRRLRAIRELNRTVEDLKNNESQWKDSPLATRHREMLKRCKTQLKKLVRCKACADAGLLDENFLRRCLNFYGIVIQLLLRILDPAYPHITLPLNPDVPKVFAALPEFYVEDVAEFLFFIVQYSPQVLYEPCTQDIAMFLVVMLCNQNYIRNPYLVAKLVEVMFMTNPAVQPRTQKFFEMIENHPLSTKLLVPSLMKFYTDVEHTGATSEFYDKFTIRYHISTIFKSLWQNIAHHGTFMEEFNSGKQFVRYINMLINDTTFLLDESLESLKRIHEVQEEMKNKEQWDLLPRDQQQARQSQLAQDERVSRSYLALATETVDMFHILTKQVQKPFLRPELGPRLAAMLNFNLQQLCGPKCRDLKVENPEKYGFEPKKLLDQLTDIYLQLDCARFAKAIADDQRSYSKELFEEVILKMRKAGIKSTIAIEKFKLLAEKVEEIVAKNARAEIDYSDAPDEFRDPLMDTLMTDPVRLPSGTIMDRSIILRHLLNSPTDPFNRQMLTESMLEPVPELKEQIQAWMREKQNTDH

Target ORF information:

RefSeq Version	XM_020974176.1
Organism	Phascolarctos cinereus(koala)
Definition	Phascolarctos cinereus ubiquitination factor E4B (UBE4B), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_020974176.1

ORF Insert Sequence:

ATGGAGGAGC TGAGCGCTGA TGAGATCCGA CGGAGGCGCC TTGCTCGACT TGCTGGTGGA 
CAGACTTCAC AGCCCTCTAC CCCACTTACC TCTCCACAGA GAGAAAACCC ACCAGGCCCT 
CCTGTAGCAG CACCAGCCCC AGGCCCTTCC CATAATCTCG GTCTAAATGT CCACAACATG 
ACCCCAGCTA CCTCCCCGAT AGGTGCATCA GGAGTCACGC ATCGAAGCCA GAGCAGTGAA 
GGAGTCAGTT CACTCAGCAG CTCACCGTCT AATAGCCTTG AAACACAGTC ACAGTCTCTC 
TCACGGTCAC AGAGCATGGA TATCGACAGT GTCTCCTGCG AGAAAAGCAT GTCCCAGGTG 
GATGTAGATT CAGGAATTGA AAATATGGAG GTTGATGAAA ATGATCGCAG AGAAAAAAGG 
ACCCTCTCTG ATAAGGAACT TTCCTCAGGT TCTGAGGTAT CAGAAGAACA GGCCTTGCAG 
CTGGTCTGTA AGATCTTTCG TGTCTCTTGG AAAGACCGGG ACAGAGACGT CATCTTTTTG 
ACTTCACTTT CTGCACAGTT TAAGCAGAAC CCAAAAGAAG TGTTCTCTGA TTTTAAGGAT 
TTGATTGGCC AGATTTTAAT GGAAGTGCTA ATGATGTCCA CTCAAACCAG AGATGAAAAT 
CCTTTTGCCA GTCTGACGGC GACGTCCCAG CCGATTGCAA CCGCAGCTCG GTCGCCAGAC 
AGAAACCTGA TGCTTAGTAC GGGATCCAAT CCAGGAACGA GCCCCATGTT CTGTAACGTG 
GGTTCCTTCA GTGCCAGCTC GCTGTCCAGC CTCTATGACA CTAGTCCAAC TCCCAATACC 
CCTTTCTCAA GCTATGTGCC AGTGACGAGC TCCTCCTGTG CCTCAGCTTC CGATGCAGCC 
GCTCCAATTT CTGTGCCTTC CATTTCGCTC AGTCCTCATA TTCCAGGAGA AAGCCCTTCA 
GGAAGCCAGG CTTCGTCTCC GAGGTATCGC CCTTACACTG TGGCCCCACC CTGGGGCTTT 
TCGACTTCTC CCACCCCGAG GTCCTCGAGC CTGTCAGCTT TATCTAGCTC ACCAAGCCCG 
CCTGCCCTGT CGACCAGCCC TGCCAGACAA AGACCCACAG CCATGGGCCC ACCCTTGTTT 
CCTGCTTCTC CAGGTCCCGT GAGCAGGCGT CCCTCTGCCC TAAGGATTCC TTCTAGTCCG 
TACGACCATC CTTTCTCCTT CCTCTTCCTC GCGCTTTCTG ATATGTCTGG GGACAGTAGT 
GATGAAGAAA ATGAAGATGG TGATGGTGAT GATGAGGATG AGGATGAAGG TGGTGGTGAT 
GATGATTTTG CTTGTGTCCA GTTTGGGTCC AGTTTGGGAG CCTCTGGTGG GGCAAGTGCT 
TCTGATTCCT GCAGTGACCA TTTCACCATT GAAACATGCA AGGAGACAGA TATGCTGAAC 
TACCTCATCG AATGTTTTGA CAGAGTTGGA ATAGAGGAAA GAAAAGCACC AAAGATGTGC 
AGCCAGCCGG CGGTCAGCCA GTTACTGAGT AACATCCGCT CACAGTGCAT CTCCCACGCT 
GCGTTAGTGT TACAGGGCTC ATTAACGCAG CCAAGGTCCA TGCAGCAGCA GTCTCTCTTG 
GTACCCTATA TGCTGTGTAG GAACCTCCCA TTTGGTTTCA TCCAAGAGCT GGTGAGAACA 
ACCCACCAGG ATGAAGAAGT GTTCAAACAG ATCTTTATCC CTATTTTACA AGGTTTGGCT 
CTTGCTGCCA AAGACTGCTC CCTTGATAGT GACTACTTTA AATACCCCCT TATGGCATTA 
GGTGAACTCT GTGAAATTAA GTTTGGGAAA ACCCATCCCA TTTGTAGTTT GGTCGCCTCT 
TTGCCTTTGT GGCTGCCGAA GTCCTTAAGC CCAGGTTCTG GGCGGGAGTT GCAAAGACTC 
TCTTATTTGG GGGCCTTCTT CAGTCTCTCC GTCTTTGCCG AGGATGATGC AAAGGTGGTT 
GAAAAGTATT TCTCAGGGCC TGCCATTACC CTAGAGAACA CCCGAGTGGT CAGCCAGTCA 
CTACAGCATT ACTTGGAGTC AGCCAGACAA GAATTGTTCA AGATTCTACA TAGTATTTTG 
CTGAATGGTG AAACTCGTGA GGCAGCTCTC AGTTACATGG CAGCTATCGT CAATGCCAAC 
ATGAAGAAAG CGCAGATGCA GACAGATGAT CGGTTGGTAT CTACGGATGG ATTTATGCTG 
AATTTCCTTT GGGTGCTGCA GCAGTTAAGC ACAAAGATCA AGCTGGAGAC GATAGATCCC 
ACCTATATAT TCCATCCGCG GTGTCGTATT GTTCTTCCGA GCGACGAGAC CCGAGTGAAG 
GCAACGATGG AGGAGGTTAC TGGTTGGATG ACTGAACTTT ATGGAGATCA GTCTCCATTT 
TCTGAGCCGA AATTCCCTAC AGAATGCTTT TTCCTTACGC TGCATGCCCA CCACCTCTCT 
ATTTTGCCAA GCTGTCGTCG TTATATACGC AGACTCCGGG CCATCCGGGA GCTTAACAGA 
ACTGTAGAAG ATCTTAAAAA CAACGAAAGT CAGTGGAAAG ATTCCCCACT GGCAACTAGA 
CATCGGGAAA TGTTGAAACG TTGCAAAACT CAACTCAAGA AATTGGTGCG ATGTAAAGCC 
TGTGCTGATG CAGGCTTGCT GGACGAAAAC TTCCTGCGGA GATGTCTGAA TTTCTATGGC 
ATCGTTATCC AGCTGCTGCT GCGTATCCTT GACCCTGCCT ATCCTCATAT CACACTGCCT 
TTAAATCCAG ATGTCCCTAA GGTGTTTGCA GCATTGCCTG AGTTTTACGT GGAAGACGTT 
GCTGAATTTT TATTTTTTAT TGTACAATAC TCCCCTCAGG TGCTGTATGA GCCCTGTACT 
CAGGATATTG CCATGTTCCT TGTTGTGATG CTGTGCAACC AGAACTATAT CCGGAATCCA 
TACCTGGTGG CCAAATTGGT GGAGGTCATG TTCATGACCA ACCCTGCTGT CCAGCCGCGG 
ACCCAGAAGT TTTTTGAAAT GATAGAGAAC CACCCTCTTT CCACCAAGCT GCTAGTCCCC 
TCACTGATGA AGTTTTATAC AGATGTTGAA CACACTGGAG CCACCAGCGA GTTCTATGAC 
AAATTTACAA TCCGATATCA TATCAGCACC ATTTTTAAAA GTCTTTGGCA AAACATAGCC 
CATCATGGCA CCTTTATGGA AGAGTTCAAT TCTGGGAAGC AGTTTGTTCG GTACATAAAC 
ATGCTGATAA ATGACACAAC GTTTTTGTTG GATGAGAGTC TGGAGTCCTT GAAGCGGATC 
CATGAAGTGC AAGAAGAAAT GAAGAACAAG GAACAGTGGG ACCTGTTGCC CCGGGACCAG 
CAGCAGGCCC GTCAGTCTCA GCTCGCTCAG GACGAGCGTG TGTCTCGCTC GTATCTTGCA 
CTAGCAACAG AAACGGTGGA TATGTTTCAT ATTCTCACCA AGCAAGTCCA GAAGCCTTTC 
CTCAGACCTG AACTTGGACC ACGCTTGGCT GCTATGCTGA ATTTTAATCT TCAGCAACTC 
TGTGGCCCCA AGTGCCGCGA CCTGAAAGTG GAAAACCCTG AGAAATATGG GTTTGAACCA 
AAGAAGCTCT TGGACCAATT GACAGATATT TACTTGCAGC TAGACTGTGC CCGCTTTGCT 
AAGGCCATTG CGGATGACCA GAGATCATAT AGCAAGGAGC TATTTGAAGA GGTGATTTTG 
AAGATGCGCA AAGCAGGAAT TAAGTCCACA ATAGCAATAG AGAAGTTTAA GCTGCTGGCA 
GAGAAGGTGG AGGAAATAGT GGCCAAGAAT GCTCGGGCCG AAATTGATTA CAGTGATGCT 
CCAGATGAAT TCAGAGACCC ATTAATGGAC ACCCTAATGA CTGACCCTGT GAGGTTGCCC 
TCTGGCACCA TCATGGACCG CTCAATCATT CTTCGGCACC TGCTGAATTC ACCCACTGAT 
CCCTTCAATC GACAGATGCT GACGGAGAGC ATGCTGGAGC CAGTGCCAGA ATTGAAAGAA 
CAAATCCAAG CCTGGATGAG AGAGAAACAG AACACTGATC ATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

UBE4B ( XM_020974185.1 ) cDNA ORF clone, Phascolarctos cinereus(koala) -> XP_020829844.1 Phascolarctos cinereus ubiquitination factor E4B (UBE4B), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OPh250263
Clone ID Related Accession (Same CDS sequence)	XM_020974185.1
Accession Version	XM_020974185.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3522bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-05-09
Organism	Phascolarctos cinereus(koala)
Product	ubiquitin conjugation factor E4 B isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018343960.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Phascolarctos cinereus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGAGC TGAGCGCTGA TGAGATCCGA CGGAGGCGCC TTGCTCGACT TGCTGGTGGA 
CAGACTTCAC AGCCCTCTAC CCCACTTACC TCTCCACAGA GAGAAAACCC ACCAGGCCCT 
CCTGTAGCAG CACCAGCCCC AGGCCCTTCC CATAATCTCG GTCTAAATGT CCACAACATG 
ACCCCAGCTA CCTCCCCGAT AGGTGCATCA GGAGTCACGC ATCGAAGCCA GAGCAGTGAA 
GGAGTCAGTT CACTCAGCAG CTCACCGTCT AATAGCCTTG AAACACAGTC ACAGTCTCTC 
TCACGGTCAC AGAGCATGGA TATCGACAGT GTCTCCTGCG AGAAAAGCAT GTCCCAGGTG 
GATGTAGATT CAGGAATTGA AAATATGGAG GTTGATGAAA ATGATCGCAG AGAAAAAAGG 
ACCCTCTCTG ATAAGGAACT TTCCTCAGGT TCTGAGGTAT CAGAAGAACA GGCCTTGCAG 
CTGGTCTGTA AGATCTTTCG TGTCTCTTGG AAAGACCGGG ACAGAGACGT CATCTTTTTG 
ACTTCACTTT CTGCACAGTT TAAGCAGAAC CCAAAAGAAG TGTTCTCTGA TTTTAAGGAT 
TTGATTGGCC AGATTTTAAT GGAAGTGCTA ATGATGTCCA CTCAAACCAG AGATGAAAAT 
CCTTTTGCCA GTCTGACGGC GACGTCCCAG CCGATTGCAA CCGCAGCTCG GTCGCCAGAC 
AGAAACCTGA TGCTTAGTAC GGGATCCAAT CCAGGAACGA GCCCCATGTT CTGTAACGTG 
GGTTCCTTCA GTGCCAGCTC GCTGTCCAGT TTGGGAGCCT CTGGTGGGGC AAGTGCTTCT 
GATTCCTGCA GTGACCATTT CACCATTGAA ACATGCAAGG AGACAGATAT GCTGAACTAC 
CTCATCGAAT GTTTTGACAG AGTTGGAATA GAGGAAAGAA AAGCACCAAA GATGTGCAGC 
CAGCCGGCGG TCAGCCAGTT ACTGAGTAAC ATCCGCTCAC AGTGCATCTC CCACGCTGCG 
TTAGTGTTAC AGGGCTCATT AACGCAGCCA AGGTCCATGC AGCAGCAGTC TCTCTTGGTA 
CCCTATATGC TGTGTAGGAA CCTCCCATTT GGTTTCATCC AAGAGCTGGT GAGAACAACC 
CACCAGGATG AAGAAGTGTT CAAACAGATC TTTATCCCTA TTTTACAAGG TTTGGCTCTT 
GCTGCCAAAG ACTGCTCCCT TGATAGTGAC TACTTTAAAT ACCCCCTTAT GGCATTAGGT 
GAACTCTGTG AAATTAAGTT TGGGAAAACC CATCCCATTT GTAGTTTGGT CGCCTCTTTG 
CCTTTGTGGC TGCCGAAGTC CTTAAGCCCA GGTTCTGGGC GGGAGTTGCA AAGACTCTCT 
TATTTGGGGG CCTTCTTCAG TCTCTCCGTC TTTGCCGAGG ATGATGCAAA GGTGGTTGAA 
AAGTATTTCT CAGGGCCTGC CATTACCCTA GAGAACACCC GAGTGGTCAG CCAGTCACTA 
CAGCATTACT TGGAGTCAGC CAGACAAGAA TTGTTCAAGA TTCTACATAG TATTTTGCTG 
AATGGTGAAA CTCGTGAGGC AGCTCTCAGT TACATGGCAG CTATCGTCAA TGCCAACATG 
AAGAAAGCGC AGATGCAGAC AGATGATCGG TTGGTATCTA CGGATGGATT TATGCTGAAT 
TTCCTTTGGG TGCTGCAGCA GTTAAGCACA AAGATCAAGC TGGAGACGAT AGATCCCACC 
TATATATTCC ATCCGCGGTG TCGTATTGTT CTTCCGAGCG ACGAGACCCG AGTGAAGGCA 
ACGATGGAGG AGGTTACTGG TTGGATGACT GAACTTTATG GAGATCAGTC TCCATTTTCT 
GAGCCGAAAT TCCCTACAGA ATGCTTTTTC CTTACGCTGC ATGCCCACCA CCTCTCTATT 
TTGCCAAGCT GTCGTCGTTA TATACGCAGA CTCCGGGCCA TCCGGGAGCT TAACAGAACT 
GTAGAAGATC TTAAAAACAA CGAAAGTCAG TGGAAAGATT CCCCACTGGC AACTAGACAT 
CGGGAAATGT TGAAACGTTG CAAAACTCAA CTCAAGAAAT TGGTGCGATG TAAAGCCTGT 
GCTGATGCAG GCTTGCTGGA CGAAAACTTC CTGCGGAGAT GTCTGAATTT CTATGGCATC 
GTTATCCAGC TGCTGCTGCG TATCCTTGAC CCTGCCTATC CTCATATCAC ACTGCCTTTA 
AATCCAGATG TCCCTAAGGT GTTTGCAGCA TTGCCTGAGT TTTACGTGGA AGACGTTGCT 
GAATTTTTAT TTTTTATTGT ACAATACTCC CCTCAGGTGC TGTATGAGCC CTGTACTCAG 
GATATTGCCA TGTTCCTTGT TGTGATGCTG TGCAACCAGA ACTATATCCG GAATCCATAC 
CTGGTGGCCA AATTGGTGGA GGTCATGTTC ATGACCAACC CTGCTGTCCA GCCGCGGACC 
CAGAAGTTTT TTGAAATGAT AGAGAACCAC CCTCTTTCCA CCAAGCTGCT AGTCCCCTCA 
CTGATGAAGT TTTATACAGA TGTTGAACAC ACTGGAGCCA CCAGCGAGTT CTATGACAAA 
TTTACAATCC GATATCATAT CAGCACCATT TTTAAAAGTC TTTGGCAAAA CATAGCCCAT 
CATGGCACCT TTATGGAAGA GTTCAATTCT GGGAAGCAGT TTGTTCGGTA CATAAACATG 
CTGATAAATG ACACAACGTT TTTGTTGGAT GAGAGTCTGG AGTCCTTGAA GCGGATCCAT 
GAAGTGCAAG AAGAAATGAA GAACAAGGAA CAGTGGGACC TGTTGCCCCG GGACCAGCAG 
CAGGCCCGTC AGTCTCAGCT CGCTCAGGAC GAGCGTGTGT CTCGCTCGTA TCTTGCACTA 
GCAACAGAAA CGGTGGATAT GTTTCATATT CTCACCAAGC AAGTCCAGAA GCCTTTCCTC 
AGACCTGAAC TTGGACCACG CTTGGCTGCT ATGCTGAATT TTAATCTTCA GCAACTCTGT 
GGCCCCAAGT GCCGCGACCT GAAAGTGGAA AACCCTGAGA AATATGGGTT TGAACCAAAG 
AAGCTCTTGG ACCAATTGAC AGATATTTAC TTGCAGCTAG ACTGTGCCCG CTTTGCTAAG 
GCCATTGCGG ATGACCAGAG ATCATATAGC AAGGAGCTAT TTGAAGAGGT GATTTTGAAG 
ATGCGCAAAG CAGGAATTAA GTCCACAATA GCAATAGAGA AGTTTAAGCT GCTGGCAGAG 
AAGGTGGAGG AAATAGTGGC CAAGAATGCT CGGGCCGAAA TTGATTACAG TGATGCTCCA 
GATGAATTCA GAGACCCATT AATGGACACC CTAATGACTG ACCCTGTGAG GTTGCCCTCT 
GGCACCATCA TGGACCGCTC AATCATTCTT CGGCACCTGC TGAATTCACC CACTGATCCC 
TTCAATCGAC AGATGCTGAC GGAGAGCATG CTGGAGCCAG TGCCAGAATT GAAAGAACAA 
ATCCAAGCCT GGATGAGAGA GAAACAGAAC ACTGATCATT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_020829844.1
CDS	503..4024
Translation	MEELSADEIRRRRLARLAGGQTSQPSTPLTSPQRENPPGPPVAAPAPGPSHNLGLNVHNMTPATSPIGASGVTHRSQSSEGVSSLSSSPSNSLETQSQSLSRSQSMDIDSVSCEKSMSQVDVDSGIENMEVDENDRREKRTLSDKELSSGSEVSEEQALQLVCKIFRVSWKDRDRDVIFLTSLSAQFKQNPKEVFSDFKDLIGQILMEVLMMSTQTRDENPFASLTATSQPIATAARSPDRNLMLSTGSNPGTSPMFCNVGSFSASSLSSLGASGGASASDSCSDHFTIETCKETDMLNYLIECFDRVGIEERKAPKMCSQPAVSQLLSNIRSQCISHAALVLQGSLTQPRSMQQQSLLVPYMLCRNLPFGFIQELVRTTHQDEEVFKQIFIPILQGLALAAKDCSLDSDYFKYPLMALGELCEIKFGKTHPICSLVASLPLWLPKSLSPGSGRELQRLSYLGAFFSLSVFAEDDAKVVEKYFSGPAITLENTRVVSQSLQHYLESARQELFKILHSILLNGETREAALSYMAAIVNANMKKAQMQTDDRLVSTDGFMLNFLWVLQQLSTKIKLETIDPTYIFHPRCRIVLPSDETRVKATMEEVTGWMTELYGDQSPFSEPKFPTECFFLTLHAHHLSILPSCRRYIRRLRAIRELNRTVEDLKNNESQWKDSPLATRHREMLKRCKTQLKKLVRCKACADAGLLDENFLRRCLNFYGIVIQLLLRILDPAYPHITLPLNPDVPKVFAALPEFYVEDVAEFLFFIVQYSPQVLYEPCTQDIAMFLVVMLCNQNYIRNPYLVAKLVEVMFMTNPAVQPRTQKFFEMIENHPLSTKLLVPSLMKFYTDVEHTGATSEFYDKFTIRYHISTIFKSLWQNIAHHGTFMEEFNSGKQFVRYINMLINDTTFLLDESLESLKRIHEVQEEMKNKEQWDLLPRDQQQARQSQLAQDERVSRSYLALATETVDMFHILTKQVQKPFLRPELGPRLAAMLNFNLQQLCGPKCRDLKVENPEKYGFEPKKLLDQLTDIYLQLDCARFAKAIADDQRSYSKELFEEVILKMRKAGIKSTIAIEKFKLLAEKVEEIVAKNARAEIDYSDAPDEFRDPLMDTLMTDPVRLPSGTIMDRSIILRHLLNSPTDPFNRQMLTESMLEPVPELKEQIQAWMREKQNTDH

Target ORF information:

RefSeq Version	XM_020974185.1
Organism	Phascolarctos cinereus(koala)
Definition	Phascolarctos cinereus ubiquitination factor E4B (UBE4B), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_020974185.1

ORF Insert Sequence:

ATGGAGGAGC TGAGCGCTGA TGAGATCCGA CGGAGGCGCC TTGCTCGACT TGCTGGTGGA 
CAGACTTCAC AGCCCTCTAC CCCACTTACC TCTCCACAGA GAGAAAACCC ACCAGGCCCT 
CCTGTAGCAG CACCAGCCCC AGGCCCTTCC CATAATCTCG GTCTAAATGT CCACAACATG 
ACCCCAGCTA CCTCCCCGAT AGGTGCATCA GGAGTCACGC ATCGAAGCCA GAGCAGTGAA 
GGAGTCAGTT CACTCAGCAG CTCACCGTCT AATAGCCTTG AAACACAGTC ACAGTCTCTC 
TCACGGTCAC AGAGCATGGA TATCGACAGT GTCTCCTGCG AGAAAAGCAT GTCCCAGGTG 
GATGTAGATT CAGGAATTGA AAATATGGAG GTTGATGAAA ATGATCGCAG AGAAAAAAGG 
ACCCTCTCTG ATAAGGAACT TTCCTCAGGT TCTGAGGTAT CAGAAGAACA GGCCTTGCAG 
CTGGTCTGTA AGATCTTTCG TGTCTCTTGG AAAGACCGGG ACAGAGACGT CATCTTTTTG 
ACTTCACTTT CTGCACAGTT TAAGCAGAAC CCAAAAGAAG TGTTCTCTGA TTTTAAGGAT 
TTGATTGGCC AGATTTTAAT GGAAGTGCTA ATGATGTCCA CTCAAACCAG AGATGAAAAT 
CCTTTTGCCA GTCTGACGGC GACGTCCCAG CCGATTGCAA CCGCAGCTCG GTCGCCAGAC 
AGAAACCTGA TGCTTAGTAC GGGATCCAAT CCAGGAACGA GCCCCATGTT CTGTAACGTG 
GGTTCCTTCA GTGCCAGCTC GCTGTCCAGT TTGGGAGCCT CTGGTGGGGC AAGTGCTTCT 
GATTCCTGCA GTGACCATTT CACCATTGAA ACATGCAAGG AGACAGATAT GCTGAACTAC 
CTCATCGAAT GTTTTGACAG AGTTGGAATA GAGGAAAGAA AAGCACCAAA GATGTGCAGC 
CAGCCGGCGG TCAGCCAGTT ACTGAGTAAC ATCCGCTCAC AGTGCATCTC CCACGCTGCG 
TTAGTGTTAC AGGGCTCATT AACGCAGCCA AGGTCCATGC AGCAGCAGTC TCTCTTGGTA 
CCCTATATGC TGTGTAGGAA CCTCCCATTT GGTTTCATCC AAGAGCTGGT GAGAACAACC 
CACCAGGATG AAGAAGTGTT CAAACAGATC TTTATCCCTA TTTTACAAGG TTTGGCTCTT 
GCTGCCAAAG ACTGCTCCCT TGATAGTGAC TACTTTAAAT ACCCCCTTAT GGCATTAGGT 
GAACTCTGTG AAATTAAGTT TGGGAAAACC CATCCCATTT GTAGTTTGGT CGCCTCTTTG 
CCTTTGTGGC TGCCGAAGTC CTTAAGCCCA GGTTCTGGGC GGGAGTTGCA AAGACTCTCT 
TATTTGGGGG CCTTCTTCAG TCTCTCCGTC TTTGCCGAGG ATGATGCAAA GGTGGTTGAA 
AAGTATTTCT CAGGGCCTGC CATTACCCTA GAGAACACCC GAGTGGTCAG CCAGTCACTA 
CAGCATTACT TGGAGTCAGC CAGACAAGAA TTGTTCAAGA TTCTACATAG TATTTTGCTG 
AATGGTGAAA CTCGTGAGGC AGCTCTCAGT TACATGGCAG CTATCGTCAA TGCCAACATG 
AAGAAAGCGC AGATGCAGAC AGATGATCGG TTGGTATCTA CGGATGGATT TATGCTGAAT 
TTCCTTTGGG TGCTGCAGCA GTTAAGCACA AAGATCAAGC TGGAGACGAT AGATCCCACC 
TATATATTCC ATCCGCGGTG TCGTATTGTT CTTCCGAGCG ACGAGACCCG AGTGAAGGCA 
ACGATGGAGG AGGTTACTGG TTGGATGACT GAACTTTATG GAGATCAGTC TCCATTTTCT 
GAGCCGAAAT TCCCTACAGA ATGCTTTTTC CTTACGCTGC ATGCCCACCA CCTCTCTATT 
TTGCCAAGCT GTCGTCGTTA TATACGCAGA CTCCGGGCCA TCCGGGAGCT TAACAGAACT 
GTAGAAGATC TTAAAAACAA CGAAAGTCAG TGGAAAGATT CCCCACTGGC AACTAGACAT 
CGGGAAATGT TGAAACGTTG CAAAACTCAA CTCAAGAAAT TGGTGCGATG TAAAGCCTGT 
GCTGATGCAG GCTTGCTGGA CGAAAACTTC CTGCGGAGAT GTCTGAATTT CTATGGCATC 
GTTATCCAGC TGCTGCTGCG TATCCTTGAC CCTGCCTATC CTCATATCAC ACTGCCTTTA 
AATCCAGATG TCCCTAAGGT GTTTGCAGCA TTGCCTGAGT TTTACGTGGA AGACGTTGCT 
GAATTTTTAT TTTTTATTGT ACAATACTCC CCTCAGGTGC TGTATGAGCC CTGTACTCAG 
GATATTGCCA TGTTCCTTGT TGTGATGCTG TGCAACCAGA ACTATATCCG GAATCCATAC 
CTGGTGGCCA AATTGGTGGA GGTCATGTTC ATGACCAACC CTGCTGTCCA GCCGCGGACC 
CAGAAGTTTT TTGAAATGAT AGAGAACCAC CCTCTTTCCA CCAAGCTGCT AGTCCCCTCA 
CTGATGAAGT TTTATACAGA TGTTGAACAC ACTGGAGCCA CCAGCGAGTT CTATGACAAA 
TTTACAATCC GATATCATAT CAGCACCATT TTTAAAAGTC TTTGGCAAAA CATAGCCCAT 
CATGGCACCT TTATGGAAGA GTTCAATTCT GGGAAGCAGT TTGTTCGGTA CATAAACATG 
CTGATAAATG ACACAACGTT TTTGTTGGAT GAGAGTCTGG AGTCCTTGAA GCGGATCCAT 
GAAGTGCAAG AAGAAATGAA GAACAAGGAA CAGTGGGACC TGTTGCCCCG GGACCAGCAG 
CAGGCCCGTC AGTCTCAGCT CGCTCAGGAC GAGCGTGTGT CTCGCTCGTA TCTTGCACTA 
GCAACAGAAA CGGTGGATAT GTTTCATATT CTCACCAAGC AAGTCCAGAA GCCTTTCCTC 
AGACCTGAAC TTGGACCACG CTTGGCTGCT ATGCTGAATT TTAATCTTCA GCAACTCTGT 
GGCCCCAAGT GCCGCGACCT GAAAGTGGAA AACCCTGAGA AATATGGGTT TGAACCAAAG 
AAGCTCTTGG ACCAATTGAC AGATATTTAC TTGCAGCTAG ACTGTGCCCG CTTTGCTAAG 
GCCATTGCGG ATGACCAGAG ATCATATAGC AAGGAGCTAT TTGAAGAGGT GATTTTGAAG 
ATGCGCAAAG CAGGAATTAA GTCCACAATA GCAATAGAGA AGTTTAAGCT GCTGGCAGAG 
AAGGTGGAGG AAATAGTGGC CAAGAATGCT CGGGCCGAAA TTGATTACAG TGATGCTCCA 
GATGAATTCA GAGACCCATT AATGGACACC CTAATGACTG ACCCTGTGAG GTTGCCCTCT 
GGCACCATCA TGGACCGCTC AATCATTCTT CGGCACCTGC TGAATTCACC CACTGATCCC 
TTCAATCGAC AGATGCTGAC GGAGAGCATG CTGGAGCCAG TGCCAGAATT GAAAGAACAA 
ATCCAAGCCT GGATGAGAGA GAAACAGAAC ACTGATCATT AG