BCKDK cDNA ORF clone, Pelodiscus sinensis(Chinese soft-shelled turtle)

Gene
Clones
gRNAs

Summary

Gene Symbol	BCKDK
Entrez Gene ID	102450847
Full Name	branched chain ketoacid dehydrogenase kinase
Gene Type	protein-coding
Organism	Pelodiscus sinensis(Chinese soft-shelled turtle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_006116426.2	XP_006116488.1	3-methyl-2-oxobutanoate dehydrogenase [lipoamide] kinase, mitochondrial
XM_006116426.3	XP_006116488.1	3-methyl-2-oxobutanoate dehydrogenase [lipoamide] kinase, mitochondrial

Pathways from BioSystems
Gene Ontology

The following BCKDK gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the BCKDK cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OPe23235	XM_006116426.3 Latest version!	Pelodiscus sinensis branched chain ketoacid dehydrogenase kinase (BCKDK), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$258.30 $369.00
View Old Accession Versions >>
OPe23235	XM_006116426.2	Pelodiscus sinensis branched chain ketoacid dehydrogenase kinase (BCKDK), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$258.30 $369.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

BCKDK ( XM_006116426.2 ) cDNA ORF clone, Pelodiscus sinensis(Chinese soft-shelled turtle) -> XP_006116488.1 Pelodiscus sinensis branched chain ketoacid dehydrogenase kinase (BCKDK), mRNA.

Price & Availability↑

CloneID	OPe23235
Clone ID Related Accession (Same CDS sequence)	XM_006116426.2 , XM_006116426.3
Accession Version	XM_006116426.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1248bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-11-02
Organism	Pelodiscus sinensis(Chinese soft-shelled turtle)
Product	3-methyl-2-oxobutanoate dehydrogenase [lipoamide] kinase, mitochondrial
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005853324.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Nov 3, 2015 this sequence version replaced XM_006116426.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pelodiscus sinensis Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGTGGA AAGCTCTGCT GAGGACGGAG CTGGGGGGAG CGGGGAACTG GACTGCCTCC 
ATGCACCGGT TCCTGTCCCC TACCCTCCGG TACCGGTCCA CTTCTGTCAT GGATAACCAC 
CAAGTGGAGC TGGCCCGAGA GCGATCCAAG ACCGTCACCT CCTTCTACAA TCAGTCGGCC 
ATCGATGTTG CAGCAGAGAA GCCCTCCGTG AGGTTGACGC CCACCACCAT GCTGTATTCG 
GGACGGTCGC AGGACGGAAG CCACCACCTG AAGAGCGCCC ACTACCTGCA CCGCGAGCTG 
CCCGTCCGCA TCGCCCATCG CATCAAGGGT TTCCGCAGCC TCCCCTTCAG CACTGGCTGC 
AACCCCACCA TCCTCCACGT GCACGAGCTG TACATCCGAG CCTTCCACAT GCTGAGTGAA 
TTCCCCCCGA TCAAGGACCA AGAGGCGGAG GCGCATTACT GCAAGCTGGT CCGGCAGCTG 
CTGGACGATC ACAAGGACGT GGTGACGCTG CTGGCCGAGG GGCTGCGGGA GTGTCGGAAA 
CACATCCAGG ACGAGAAAGT CATCCGCTAT TTCCTAGACA AGACGCTCAC CTCCCGGCTG 
GGGATCCGGA TGCTGGCGAT CCATCACCTG GCGCTGCACG AGGAAAAGGT GCGATTCCTC 
TCTCTCGCTG GCCCCCGCCT CTCCCCGAAG AAAATCATCG AGAAATGGGT GGATTTTGCG 
AGGCGCCTGT GTGAGCACAA GTATGGGAAC GCCCCGCGGG TGCGGATCAA CGGGCACGTG 
GCCGCTCGCT TCCCCTTCAT CCCCATGCCC CTCGACTACA TCCTGCCGGA GCTGCTCAAG 
AACGCCATGA GAGCCACCAT GGAATCCCAT CTGGACACTC CCTACAACGT ACCCGACATT 
GTCATCACCA TCGCCAACAA CGACATCGAC CTGATCATCA GGATTTCGGA CCGGGGCGGC 
GGGATTCCGC ACGAACACCT GGAGAAGGTG ATGGATTATC ACTTCACCAC GGCGGAGTCC 
AGCACCCAGG ACCCCCGCAT GAACACCCTC TTTGGGAACA TGGTGGACAT GGTCAACAGC 
GGCCAGGCGG GCCCCATGCA CGGGTTCGGC TTTGGGCTGC CGACTTCTCG TGCCTACGCC 
GAGTACCTGG GGGGCTCGCT GCACATCCAG TCCCTGCAGG GTATCGGTAC CGATGTTTAT 
CTCCGGCTGA AACACATCGA TGGCAAGGAG GAGAGTTTCC GCATCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006116488.1
CDS	165..1412
Translation	MLWKALLRTELGGAGNWTASMHRFLSPTLRYRSTSVMDNHQVELARERSKTVTSFYNQSAIDVAAEKPSVRLTPTTMLYSGRSQDGSHHLKSAHYLHRELPVRIAHRIKGFRSLPFSTGCNPTILHVHELYIRAFHMLSEFPPIKDQEAEAHYCKLVRQLLDDHKDVVTLLAEGLRECRKHIQDEKVIRYFLDKTLTSRLGIRMLAIHHLALHEEKVRFLSLAGPRLSPKKIIEKWVDFARRLCEHKYGNAPRVRINGHVAARFPFIPMPLDYILPELLKNAMRATMESHLDTPYNVPDIVITIANNDIDLIIRISDRGGGIPHEHLEKVMDYHFTTAESSTQDPRMNTLFGNMVDMVNSGQAGPMHGFGFGLPTSRAYAEYLGGSLHIQSLQGIGTDVYLRLKHIDGKEESFRI

Target ORF information:

RefSeq Version	XM_006116426.2
Organism	Pelodiscus sinensis(Chinese soft-shelled turtle)
Definition	Pelodiscus sinensis branched chain ketoacid dehydrogenase kinase (BCKDK), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006116426.2

ORF Insert Sequence:

ATGCTGTGGA AAGCTCTGCT GAGGACGGAG CTGGGGGGAG CGGGGAACTG GACTGCCTCC 
ATGCACCGGT TCCTGTCCCC TACCCTCCGG TACCGGTCCA CTTCTGTCAT GGATAACCAC 
CAAGTGGAGC TGGCCCGAGA GCGATCCAAG ACCGTCACCT CCTTCTACAA TCAGTCGGCC 
ATCGATGTTG CAGCAGAGAA GCCCTCCGTG AGGTTGACGC CCACCACCAT GCTGTATTCG 
GGACGGTCGC AGGACGGAAG CCACCACCTG AAGAGCGCCC ACTACCTGCA CCGCGAGCTG 
CCCGTCCGCA TCGCCCATCG CATCAAGGGT TTCCGCAGCC TCCCCTTCAG CACTGGCTGC 
AACCCCACCA TCCTCCACGT GCACGAGCTG TACATCCGAG CCTTCCACAT GCTGAGTGAA 
TTCCCCCCGA TCAAGGACCA AGAGGCGGAG GCGCATTACT GCAAGCTGGT CCGGCAGCTG 
CTGGACGATC ACAAGGACGT GGTGACGCTG CTGGCCGAGG GGCTGCGGGA GTGTCGGAAA 
CACATCCAGG ACGAGAAAGT CATCCGCTAT TTCCTAGACA AGACGCTCAC CTCCCGGCTG 
GGGATCCGGA TGCTGGCGAT CCATCACCTG GCGCTGCACG AGGAAAAGGT GCGATTCCTC 
TCTCTCGCTG GCCCCCGCCT CTCCCCGAAG AAAATCATCG AGAAATGGGT GGATTTTGCG 
AGGCGCCTGT GTGAGCACAA GTATGGGAAC GCCCCGCGGG TGCGGATCAA CGGGCACGTG 
GCCGCTCGCT TCCCCTTCAT CCCCATGCCC CTCGACTACA TCCTGCCGGA GCTGCTCAAG 
AACGCCATGA GAGCCACCAT GGAATCCCAT CTGGACACTC CCTACAACGT ACCCGACATT 
GTCATCACCA TCGCCAACAA CGACATCGAC CTGATCATCA GGATTTCGGA CCGGGGCGGC 
GGGATTCCGC ACGAACACCT GGAGAAGGTG ATGGATTATC ACTTCACCAC GGCGGAGTCC 
AGCACCCAGG ACCCCCGCAT GAACACCCTC TTTGGGAACA TGGTGGACAT GGTCAACAGC 
GGCCAGGCGG GCCCCATGCA CGGGTTCGGC TTTGGGCTGC CGACTTCTCG TGCCTACGCC 
GAGTACCTGG GGGGCTCGCT GCACATCCAG TCCCTGCAGG GTATCGGTAC CGATGTTTAT 
CTCCGGCTGA AACACATCGA TGGCAAGGAG GAGAGTTTCC GCATCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

BCKDK ( XM_006116426.3 ) cDNA ORF clone, Pelodiscus sinensis(Chinese soft-shelled turtle) -> XP_006116488.1 Pelodiscus sinensis branched chain ketoacid dehydrogenase kinase (BCKDK), mRNA.

Price & Availability↑

CloneID	OPe23235
Clone ID Related Accession (Same CDS sequence)	XM_006116426.2 , XM_006116426.3
Accession Version	XM_006116426.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1248bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-03
Organism	Pelodiscus sinensis(Chinese soft-shelled turtle)
Product	3-methyl-2-oxobutanoate dehydrogenase [lipoamide] kinase, mitochondrial
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005853324.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 4, 2018 this sequence version replaced XM_006116426.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pelodiscus sinensis Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGTGGA AAGCTCTGCT GAGGACGGAG CTGGGGGGAG CGGGGAACTG GACTGCCTCC 
ATGCACCGGT TCCTGTCCCC TACCCTCCGG TACCGGTCCA CTTCTGTCAT GGATAACCAC 
CAAGTGGAGC TGGCCCGAGA GCGATCCAAG ACCGTCACCT CCTTCTACAA TCAGTCGGCC 
ATCGATGTTG CAGCAGAGAA GCCCTCCGTG AGGTTGACGC CCACCACCAT GCTGTATTCG 
GGACGGTCGC AGGACGGAAG CCACCACCTG AAGAGCGCCC ACTACCTGCA CCGCGAGCTG 
CCCGTCCGCA TCGCCCATCG CATCAAGGGT TTCCGCAGCC TCCCCTTCAG CACTGGCTGC 
AACCCCACCA TCCTCCACGT GCACGAGCTG TACATCCGAG CCTTCCACAT GCTGAGTGAA 
TTCCCCCCGA TCAAGGACCA AGAGGCGGAG GCGCATTACT GCAAGCTGGT CCGGCAGCTG 
CTGGACGATC ACAAGGACGT GGTGACGCTG CTGGCCGAGG GGCTGCGGGA GTGTCGGAAA 
CACATCCAGG ACGAGAAAGT CATCCGCTAT TTCCTAGACA AGACGCTCAC CTCCCGGCTG 
GGGATCCGGA TGCTGGCGAT CCATCACCTG GCGCTGCACG AGGAAAAGGT GCGATTCCTC 
TCTCTCGCTG GCCCCCGCCT CTCCCCGAAG AAAATCATCG AGAAATGGGT GGATTTTGCG 
AGGCGCCTGT GTGAGCACAA GTATGGGAAC GCCCCGCGGG TGCGGATCAA CGGGCACGTG 
GCCGCTCGCT TCCCCTTCAT CCCCATGCCC CTCGACTACA TCCTGCCGGA GCTGCTCAAG 
AACGCCATGA GAGCCACCAT GGAATCCCAT CTGGACACTC CCTACAACGT ACCCGACATT 
GTCATCACCA TCGCCAACAA CGACATCGAC CTGATCATCA GGATTTCGGA CCGGGGCGGC 
GGGATTCCGC ACGAACACCT GGAGAAGGTG ATGGATTATC ACTTCACCAC GGCGGAGTCC 
AGCACCCAGG ACCCCCGCAT GAACACCCTC TTTGGGAACA TGGTGGACAT GGTCAACAGC 
GGCCAGGCGG GCCCCATGCA CGGGTTCGGC TTTGGGCTGC CGACTTCTCG TGCCTACGCC 
GAGTACCTGG GGGGCTCGCT GCACATCCAG TCCCTGCAGG GTATCGGTAC CGATGTTTAT 
CTCCGGCTGA AACACATCGA TGGCAAGGAG GAGAGTTTCC GCATCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006116488.1
CDS	170..1417
Translation	MLWKALLRTELGGAGNWTASMHRFLSPTLRYRSTSVMDNHQVELARERSKTVTSFYNQSAIDVAAEKPSVRLTPTTMLYSGRSQDGSHHLKSAHYLHRELPVRIAHRIKGFRSLPFSTGCNPTILHVHELYIRAFHMLSEFPPIKDQEAEAHYCKLVRQLLDDHKDVVTLLAEGLRECRKHIQDEKVIRYFLDKTLTSRLGIRMLAIHHLALHEEKVRFLSLAGPRLSPKKIIEKWVDFARRLCEHKYGNAPRVRINGHVAARFPFIPMPLDYILPELLKNAMRATMESHLDTPYNVPDIVITIANNDIDLIIRISDRGGGIPHEHLEKVMDYHFTTAESSTQDPRMNTLFGNMVDMVNSGQAGPMHGFGFGLPTSRAYAEYLGGSLHIQSLQGIGTDVYLRLKHIDGKEESFRI

Target ORF information:

RefSeq Version	XM_006116426.3
Organism	Pelodiscus sinensis(Chinese soft-shelled turtle)
Definition	Pelodiscus sinensis branched chain ketoacid dehydrogenase kinase (BCKDK), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006116426.3

ORF Insert Sequence:

ATGCTGTGGA AAGCTCTGCT GAGGACGGAG CTGGGGGGAG CGGGGAACTG GACTGCCTCC 
ATGCACCGGT TCCTGTCCCC TACCCTCCGG TACCGGTCCA CTTCTGTCAT GGATAACCAC 
CAAGTGGAGC TGGCCCGAGA GCGATCCAAG ACCGTCACCT CCTTCTACAA TCAGTCGGCC 
ATCGATGTTG CAGCAGAGAA GCCCTCCGTG AGGTTGACGC CCACCACCAT GCTGTATTCG 
GGACGGTCGC AGGACGGAAG CCACCACCTG AAGAGCGCCC ACTACCTGCA CCGCGAGCTG 
CCCGTCCGCA TCGCCCATCG CATCAAGGGT TTCCGCAGCC TCCCCTTCAG CACTGGCTGC 
AACCCCACCA TCCTCCACGT GCACGAGCTG TACATCCGAG CCTTCCACAT GCTGAGTGAA 
TTCCCCCCGA TCAAGGACCA AGAGGCGGAG GCGCATTACT GCAAGCTGGT CCGGCAGCTG 
CTGGACGATC ACAAGGACGT GGTGACGCTG CTGGCCGAGG GGCTGCGGGA GTGTCGGAAA 
CACATCCAGG ACGAGAAAGT CATCCGCTAT TTCCTAGACA AGACGCTCAC CTCCCGGCTG 
GGGATCCGGA TGCTGGCGAT CCATCACCTG GCGCTGCACG AGGAAAAGGT GCGATTCCTC 
TCTCTCGCTG GCCCCCGCCT CTCCCCGAAG AAAATCATCG AGAAATGGGT GGATTTTGCG 
AGGCGCCTGT GTGAGCACAA GTATGGGAAC GCCCCGCGGG TGCGGATCAA CGGGCACGTG 
GCCGCTCGCT TCCCCTTCAT CCCCATGCCC CTCGACTACA TCCTGCCGGA GCTGCTCAAG 
AACGCCATGA GAGCCACCAT GGAATCCCAT CTGGACACTC CCTACAACGT ACCCGACATT 
GTCATCACCA TCGCCAACAA CGACATCGAC CTGATCATCA GGATTTCGGA CCGGGGCGGC 
GGGATTCCGC ACGAACACCT GGAGAAGGTG ATGGATTATC ACTTCACCAC GGCGGAGTCC 
AGCACCCAGG ACCCCCGCAT GAACACCCTC TTTGGGAACA TGGTGGACAT GGTCAACAGC 
GGCCAGGCGG GCCCCATGCA CGGGTTCGGC TTTGGGCTGC CGACTTCTCG TGCCTACGCC 
GAGTACCTGG GGGGCTCGCT GCACATCCAG TCCCTGCAGG GTATCGGTAC CGATGTTTAT 
CTCCGGCTGA AACACATCGA TGGCAAGGAG GAGAGTTTCC GCATCTAG