TOPORS cDNA ORF clone, Pelodiscus sinensis(Chinese soft-shelled turtle)

Gene
Clones
gRNAs

Summary

Gene Symbol	TOPORS
Entrez Gene ID	102444441
Full Name	TOP1 binding arginine/serine rich protein
Gene Type	protein-coding
Organism	Pelodiscus sinensis(Chinese soft-shelled turtle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_025182076.1	XP_025037861.1	E3 ubiquitin-protein ligase Topors
XM_006117984.2	XP_006118046.1	E3 ubiquitin-protein ligase Topors

Pathways from BioSystems
Gene Ontology

The following TOPORS gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TOPORS cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OPe149099	XM_025182076.1 Latest version!	Pelodiscus sinensis TOP1 binding arginine/serine rich protein (TOPORS), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OPe24680	XM_006117984.2 Latest version!	Pelodiscus sinensis topoisomerase I binding, arginine/serine-rich, E3 ubiquitin protein ligase (TOPORS), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

TOPORS ( XM_025182076.1 ) cDNA ORF clone, Pelodiscus sinensis(Chinese soft-shelled turtle) -> XP_025037861.1 Pelodiscus sinensis TOP1 binding arginine/serine rich protein (TOPORS), mRNA.

Price & Availability↑

CloneID	OPe149099
Clone ID Related Accession (Same CDS sequence)	XM_025182076.1
Accession Version	XM_025182076.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3075bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-03
Organism	Pelodiscus sinensis(Chinese soft-shelled turtle)
Product	E3 ubiquitin-protein ligase Topors
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005853731.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pelodiscus sinensis Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACCCAT GTGTGCCACC AGGAGCAGGG CACCAGAACA TGATATCATC AAGTGAGGAG 
TTTACAGTGG ATAGCAGTTT TTCACCGAAA GCTGGCACAA GCAAGCTGCA TCAGACAGTG 
CCAACAGATA CATCTCCAGA TTCTAAATGT CCAATCTGCC TGGACAGATT TGACAACGTG 
GCCTACTTAG ATCGTTGCTT GCATAGGTTC TGCTTTCGTT GTGTACAGGA ATGGTCAAAA 
AACAAAGCTG AATGTCCACT CTGCAAACAA CCTTTCCATT CTATCTTCCA TTCAGTGAGA 
GCTGAAGATG ATTTCAAGGA GTATGTACTC AGGCCTTTAC AGAATGGCTC TTTTGCTAGT 
CCTGATGGGC GGAGATTTCG TTACCGTACT ACGTTAACAA GGGAACGGCA TACACCAGCT 
TATCCTCGAA GAAGTTCCTC TTCTCGAAGG ACAGTGTCAC CTCCAGATAA TGGGATATTA 
TTTGAAGGAT TATCCAGTCA ACCAGTACGA CAAAGAGATG GAGGAATTCA TCAGATGATT 
AGACGGCTTG CATCAAGGAG ACAAGCTAGT GCAGAGGGTA GGTCGCTGCG GCAAATTCAA 
GAACAAGATA TAATTAATTT CCGAAGAGCT CTGTATCGTT CTGGTGTGCG TGTTAGAAAT 
ATTCAAGATG GTGGTCGTTA CAGAGACATT TCAGCTGAGT TTTTCCGCAG GAACCCTGCC 
TGTCTTCACA GATTAGTTCC ATGGCTAAAG CGTGAGTTAA CTGTCCTGTT TGGTGCCCAT 
GGATCTCTAG TCAATATTGT ACAGCATATC ATTATGAGTA ATGTGACTAG ATACGATTTG 
GAAAGTCAGG CCTTTGCTGA TGACTTAAAG CCTTTCTTGC TCCATCGTAC TAACCACTTT 
TTACATGAAT TCATCAGCTT TGCCCGATGT CCTTTTAACA TAGAGGCATA TGACCAGCAT 
GCCAATTATG ATTGTCCTGC TCCTTCATAT GAAGAAGGAA GCCAGTCGGA ATCATCTGTT 
ATTACGATAT CTCCAGATGA AACAGATTCT CAAGAACCAG ATCATAATGC ATCCACGACT 
AGTGTTGGTC GAGCACCCTG GGATGATGAA ACTCCAGGGC CATCGTATTC CACCTCAGAG 
CAGGTTCATG TTGCCATGGC TTCTCCTTTG GATACTTCAG AAACTTCTGA TGAAGAACCC 
ACTAGAAATG GAACTGAGCT GCAAGGGCAG TTACAAGATA ATACAGCCCC AAATGGTGAG 
AGTGATTCTT GTTCAGACAA CTGCGTCATT GTTGGGTATG TTAAGCCCTT AGCTGAAAGA 
ACACCAGAAC TTGTTGAGCT GTCCTCAGAC TCTGAAGAGT CAGTTGATGA TGGGAAAAGC 
GAGGATGTGA AGAAATCACA GCCCATCCAG TTTCATGTTT TTAGTGTCAG TGATGCTAGT 
GGGTGTTCAT CACCTTGCTC TCCTATGTCT AAGGATGGTA AATGTAGCCA TAAAAGCAGC 
GCCTCACCCT CACGTAAGAA GATGCAGTCC AAAAAGATTG AGGAGGATGT AAATAAACCA 
AAAGATTCAT CTACAAAGGA CTCATTACAA AGTTCTTCTA CAAGACGGGA TAGGATGTGC 
TCTCCATGTA GCCACAGATT ATCCAGGAGG AGGAGGTCAA GGAGTTCAGA ATTATATTCA 
CAGCATTCTC ACTACAGAGA CGGCCATGGC CAGAGAACCA GGAGGAAACA AGGCAGCAAA 
GAGAGGTTAA AAAGTGGACC ATCAAGAAGC AGAGACAGTA GCAGACATAG GAGTAAAAAA 
GACAAGAGGA GATCAAGACC TCGAGACATA AGTTTATCTA GAAAAAGCCA GACCATGTCT 
CTAAGTAGTG AGAGCACTAT ATCTAGAGAT GTAAGCAGGT CCCGGTCCCG GAGTAATGAC 
CATAGCAGAA GAAGATCAAG GAGCAGAGAT AGCGATCATT ATTATATGAG AGATAATTAC 
CAAAGTAGAT ACCAGTGGGA ATACACTTTT TACAGTAGAA ACATGGACAG AGATGGTTAT 
GAATCCTCAT ACAGGAGGAG AACTGAGGCA AGAGCTCATT ATTCAAGGCA ATCTGCTAGT 
CCAGAGTACA GAATACAATC GTTTTCTGAA AGGACAAATA CTCGAAATCA GAGAGGTCAC 
AATGAAAGCC GGTATTATTA CTATGAAAGA CACCGATCAA GGAGTCGATC CAGTAATAGA 
TCTCGGACTA CTTCTGATAG AATGCGATGT GAAAAGCCTG GTGGAAAAAG GAAATACAAA 
ACTCGCCACT TGGAGAATGC ACACAAGGAG AATGAGACAC GTTCGTGTGA AGTGAAAGGA 
AGTGACCTCC AGAAGCCTTT GTCAAAATAT AGTGGTTGCT ACAAAAATGA GGACAGCATT 
TTAGACAATC AAGCCAGTGT TGGGACTAGG CATAAAAAGA GGAAGAAGAA GACAAGAAGT 
CCAAGTGTAG AGATTATTTA TGAAGGAAGA GCGACTGATA CAACCAGACA TCATAAAAAG 
AAAAAGAAAA AGCACAAGAA GAAACACAGG CGACATCATA TGGGTAACTC GTCACATTCT 
TCTCCAGTTG TGATTACAAT TGACAGTGAT AGTGATAAGG AGACTGCAAT ACAAGAAAAC 
ACTGAATGTG ACAGTAATAT CTCTTGGACA ACTACAACCC AGATATATGA GAGAGATAAT 
GAGACTCCAT CCCCTGTCCT GGGAACAAGT GACTGTAAGG ATGAGGACAC AGTTGCTGGT 
GAAGGTGGAT TGCTGGACAA GGATTCTTGT ATTAATATCA CTAGGGGGGA CTTAGCTGAT 
GAAATTGGAA ATGTAGGTTG CCAATTTCAG GAAATATCAA ATGAGCAGGC TCTTGCAGTG 
GCAGATGGTG GTAGTGCATC TGACATGGGA ACTCCTCTGA GCAATGTTGA AATTATACAA 
ACTGAATCAA GTCAGTTGCC TTCTTCCAGA GCATCATTTG TAGGGAATTC GGAGAGACCT 
CCTTTGATAC TAAGGCTGCC AAAGAGACTT ATTGACAGAT CCTATTGGCT TGACTCCCCA 
GAAAAAAAGA TGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025037861.1
CDS	388..3462
Translation	MNPCVPPGAGHQNMISSSEEFTVDSSFSPKAGTSKLHQTVPTDTSPDSKCPICLDRFDNVAYLDRCLHRFCFRCVQEWSKNKAECPLCKQPFHSIFHSVRAEDDFKEYVLRPLQNGSFASPDGRRFRYRTTLTRERHTPAYPRRSSSSRRTVSPPDNGILFEGLSSQPVRQRDGGIHQMIRRLASRRQASAEGRSLRQIQEQDIINFRRALYRSGVRVRNIQDGGRYRDISAEFFRRNPACLHRLVPWLKRELTVLFGAHGSLVNIVQHIIMSNVTRYDLESQAFADDLKPFLLHRTNHFLHEFISFARCPFNIEAYDQHANYDCPAPSYEEGSQSESSVITISPDETDSQEPDHNASTTSVGRAPWDDETPGPSYSTSEQVHVAMASPLDTSETSDEEPTRNGTELQGQLQDNTAPNGESDSCSDNCVIVGYVKPLAERTPELVELSSDSEESVDDGKSEDVKKSQPIQFHVFSVSDASGCSSPCSPMSKDGKCSHKSSASPSRKKMQSKKIEEDVNKPKDSSTKDSLQSSSTRRDRMCSPCSHRLSRRRRSRSSELYSQHSHYRDGHGQRTRRKQGSKERLKSGPSRSRDSSRHRSKKDKRRSRPRDISLSRKSQTMSLSSESTISRDVSRSRSRSNDHSRRRSRSRDSDHYYMRDNYQSRYQWEYTFYSRNMDRDGYESSYRRRTEARAHYSRQSASPEYRIQSFSERTNTRNQRGHNESRYYYYERHRSRSRSSNRSRTTSDRMRCEKPGGKRKYKTRHLENAHKENETRSCEVKGSDLQKPLSKYSGCYKNEDSILDNQASVGTRHKKRKKKTRSPSVEIIYEGRATDTTRHHKKKKKKHKKKHRRHHMGNSSHSSPVVITIDSDSDKETAIQENTECDSNISWTTTTQIYERDNETPSPVLGTSDCKDEDTVAGEGGLLDKDSCINITRGDLADEIGNVGCQFQEISNEQALAVADGGSASDMGTPLSNVEIIQTESSQLPSSRASFVGNSERPPLILRLPKRLIDRSYWLDSPEKKM

Target ORF information:

RefSeq Version	XM_025182076.1
Organism	Pelodiscus sinensis(Chinese soft-shelled turtle)
Definition	Pelodiscus sinensis TOP1 binding arginine/serine rich protein (TOPORS), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025182076.1

ORF Insert Sequence:

ATGAACCCAT GTGTGCCACC AGGAGCAGGG CACCAGAACA TGATATCATC AAGTGAGGAG 
TTTACAGTGG ATAGCAGTTT TTCACCGAAA GCTGGCACAA GCAAGCTGCA TCAGACAGTG 
CCAACAGATA CATCTCCAGA TTCTAAATGT CCAATCTGCC TGGACAGATT TGACAACGTG 
GCCTACTTAG ATCGTTGCTT GCATAGGTTC TGCTTTCGTT GTGTACAGGA ATGGTCAAAA 
AACAAAGCTG AATGTCCACT CTGCAAACAA CCTTTCCATT CTATCTTCCA TTCAGTGAGA 
GCTGAAGATG ATTTCAAGGA GTATGTACTC AGGCCTTTAC AGAATGGCTC TTTTGCTAGT 
CCTGATGGGC GGAGATTTCG TTACCGTACT ACGTTAACAA GGGAACGGCA TACACCAGCT 
TATCCTCGAA GAAGTTCCTC TTCTCGAAGG ACAGTGTCAC CTCCAGATAA TGGGATATTA 
TTTGAAGGAT TATCCAGTCA ACCAGTACGA CAAAGAGATG GAGGAATTCA TCAGATGATT 
AGACGGCTTG CATCAAGGAG ACAAGCTAGT GCAGAGGGTA GGTCGCTGCG GCAAATTCAA 
GAACAAGATA TAATTAATTT CCGAAGAGCT CTGTATCGTT CTGGTGTGCG TGTTAGAAAT 
ATTCAAGATG GTGGTCGTTA CAGAGACATT TCAGCTGAGT TTTTCCGCAG GAACCCTGCC 
TGTCTTCACA GATTAGTTCC ATGGCTAAAG CGTGAGTTAA CTGTCCTGTT TGGTGCCCAT 
GGATCTCTAG TCAATATTGT ACAGCATATC ATTATGAGTA ATGTGACTAG ATACGATTTG 
GAAAGTCAGG CCTTTGCTGA TGACTTAAAG CCTTTCTTGC TCCATCGTAC TAACCACTTT 
TTACATGAAT TCATCAGCTT TGCCCGATGT CCTTTTAACA TAGAGGCATA TGACCAGCAT 
GCCAATTATG ATTGTCCTGC TCCTTCATAT GAAGAAGGAA GCCAGTCGGA ATCATCTGTT 
ATTACGATAT CTCCAGATGA AACAGATTCT CAAGAACCAG ATCATAATGC ATCCACGACT 
AGTGTTGGTC GAGCACCCTG GGATGATGAA ACTCCAGGGC CATCGTATTC CACCTCAGAG 
CAGGTTCATG TTGCCATGGC TTCTCCTTTG GATACTTCAG AAACTTCTGA TGAAGAACCC 
ACTAGAAATG GAACTGAGCT GCAAGGGCAG TTACAAGATA ATACAGCCCC AAATGGTGAG 
AGTGATTCTT GTTCAGACAA CTGCGTCATT GTTGGGTATG TTAAGCCCTT AGCTGAAAGA 
ACACCAGAAC TTGTTGAGCT GTCCTCAGAC TCTGAAGAGT CAGTTGATGA TGGGAAAAGC 
GAGGATGTGA AGAAATCACA GCCCATCCAG TTTCATGTTT TTAGTGTCAG TGATGCTAGT 
GGGTGTTCAT CACCTTGCTC TCCTATGTCT AAGGATGGTA AATGTAGCCA TAAAAGCAGC 
GCCTCACCCT CACGTAAGAA GATGCAGTCC AAAAAGATTG AGGAGGATGT AAATAAACCA 
AAAGATTCAT CTACAAAGGA CTCATTACAA AGTTCTTCTA CAAGACGGGA TAGGATGTGC 
TCTCCATGTA GCCACAGATT ATCCAGGAGG AGGAGGTCAA GGAGTTCAGA ATTATATTCA 
CAGCATTCTC ACTACAGAGA CGGCCATGGC CAGAGAACCA GGAGGAAACA AGGCAGCAAA 
GAGAGGTTAA AAAGTGGACC ATCAAGAAGC AGAGACAGTA GCAGACATAG GAGTAAAAAA 
GACAAGAGGA GATCAAGACC TCGAGACATA AGTTTATCTA GAAAAAGCCA GACCATGTCT 
CTAAGTAGTG AGAGCACTAT ATCTAGAGAT GTAAGCAGGT CCCGGTCCCG GAGTAATGAC 
CATAGCAGAA GAAGATCAAG GAGCAGAGAT AGCGATCATT ATTATATGAG AGATAATTAC 
CAAAGTAGAT ACCAGTGGGA ATACACTTTT TACAGTAGAA ACATGGACAG AGATGGTTAT 
GAATCCTCAT ACAGGAGGAG AACTGAGGCA AGAGCTCATT ATTCAAGGCA ATCTGCTAGT 
CCAGAGTACA GAATACAATC GTTTTCTGAA AGGACAAATA CTCGAAATCA GAGAGGTCAC 
AATGAAAGCC GGTATTATTA CTATGAAAGA CACCGATCAA GGAGTCGATC CAGTAATAGA 
TCTCGGACTA CTTCTGATAG AATGCGATGT GAAAAGCCTG GTGGAAAAAG GAAATACAAA 
ACTCGCCACT TGGAGAATGC ACACAAGGAG AATGAGACAC GTTCGTGTGA AGTGAAAGGA 
AGTGACCTCC AGAAGCCTTT GTCAAAATAT AGTGGTTGCT ACAAAAATGA GGACAGCATT 
TTAGACAATC AAGCCAGTGT TGGGACTAGG CATAAAAAGA GGAAGAAGAA GACAAGAAGT 
CCAAGTGTAG AGATTATTTA TGAAGGAAGA GCGACTGATA CAACCAGACA TCATAAAAAG 
AAAAAGAAAA AGCACAAGAA GAAACACAGG CGACATCATA TGGGTAACTC GTCACATTCT 
TCTCCAGTTG TGATTACAAT TGACAGTGAT AGTGATAAGG AGACTGCAAT ACAAGAAAAC 
ACTGAATGTG ACAGTAATAT CTCTTGGACA ACTACAACCC AGATATATGA GAGAGATAAT 
GAGACTCCAT CCCCTGTCCT GGGAACAAGT GACTGTAAGG ATGAGGACAC AGTTGCTGGT 
GAAGGTGGAT TGCTGGACAA GGATTCTTGT ATTAATATCA CTAGGGGGGA CTTAGCTGAT 
GAAATTGGAA ATGTAGGTTG CCAATTTCAG GAAATATCAA ATGAGCAGGC TCTTGCAGTG 
GCAGATGGTG GTAGTGCATC TGACATGGGA ACTCCTCTGA GCAATGTTGA AATTATACAA 
ACTGAATCAA GTCAGTTGCC TTCTTCCAGA GCATCATTTG TAGGGAATTC GGAGAGACCT 
CCTTTGATAC TAAGGCTGCC AAAGAGACTT ATTGACAGAT CCTATTGGCT TGACTCCCCA 
GAAAAAAAGA TGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TOPORS ( XM_006117984.2 ) cDNA ORF clone, Pelodiscus sinensis(Chinese soft-shelled turtle) -> XP_006118046.1 Pelodiscus sinensis topoisomerase I binding, arginine/serine-rich, E3 ubiquitin protein ligase (TOPORS), mRNA.

Price & Availability↑

CloneID	OPe24680
Clone ID Related Accession (Same CDS sequence)	XM_006117984.2
Accession Version	XM_006117984.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3036bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-11-02
Organism	Pelodiscus sinensis(Chinese soft-shelled turtle)
Product	E3 ubiquitin-protein ligase Topors
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005853731.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Nov 3, 2015 this sequence version replaced XM_006117984.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pelodiscus sinensis Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATATCAT CAAGTGAGGA GTTTACAGTG GATAGCAGTT TTTCACCGAA AGCTGGCACA 
AGCAAGCTGC ATCAGACAGT GCCAACAGAT ACATCTCCAG ATTCTAAATG TCCAATCTGC 
CTGGACAGAT TTGACAACGT GGCCTACTTA GATCGTTGCT TGCATAGGTT CTGCTTTCGT 
TGTGTACAGG AATGGTCAAA AAACAAAGCT GAATGTCCAC TCTGCAAACA ACCTTTCCAT 
TCTATCTTCC ATTCAGTGAG AGCTGAAGAT GATTTCAAGG AGTATGTACT CAGGCCTTTA 
CAGAATGGCT CTTTTGCTAG TCCTGATGGG CGGAGATTTC GTTACCGTAC TACGTTAACA 
AGGGAACGGC ATACACCAGC TTATCCTCGA AGAAGTTCCT CTTCTCGAAG GACAGTGTCA 
CCTCCAGATA ATGGGATATT ATTTGAAGGA TTATCCAGTC AACCAGTACG ACAAAGAGAT 
GGAGGAATTC ATCAGATGAT TAGACGGCTT GCATCAAGGA GACAAGCTAG TGCAGAGGGT 
AGGTCGCTGC GGCAAATTCA AGAACAAGAT ATAATTAATT TCCGAAGAGC TCTGTATCGT 
TCTGGTGTGC GTGTTAGAAA TATTCAAGAT GGTGGTCGTT ACAGAGACAT TTCAGCTGAG 
TTTTTCCGCA GGAACCCTGC CTGTCTTCAC AGATTAGTTC CATGGCTAAA GCGTGAGTTA 
ACTGTCCTGT TTGGTGCCCA TGGATCTCTA GTCAATATTG TACAGCATAT CATTATGAGT 
AATGTGACTA GATACGATTT GGAAAGTCAG GCCTTTGCTG ATGACTTAAA GCCTTTCTTG 
CTCCATCGTA CTAACCACTT TTTACATGAA TTCATCAGCT TTGCCCGATG TCCTTTTAAC 
ATAGAGGCAT ATGACCAGCA TGCCAATTAT GATTGTCCTG CTCCTTCATA TGAAGAAGGA 
AGCCAGTCGG AATCATCTGT TATTACGATA TCTCCAGATG AAACAGATTC TCAAGAACCA 
GATCATAATG CATCCACGAC TAGTGTTGGT CGAGCACCCT GGGATGATGA AACTCCAGGG 
CCATCGTATT CCACCTCAGA GCAGGTTCAT GTTGCCATGG CTTCTCCTTT GGATACTTCA 
GAAACTTCTG ATGAAGAACC CACTAGAAAT GGAACTGAGC TGCAAGGGCA GTTACAAGAT 
AATACAGCCC CAAATGGTGA GAGTGATTCT TGTTCAGACA ACTGCGTCAT TGTTGGGTAT 
GTTAAGCCCT TAGCTGAAAG AACACCAGAA CTTGTTGAGC TGTCCTCAGA CTCTGAAGAG 
TCAGTTGATG ATGGGAAAAG CGAGGATGTG AAGAAATCAC AGCCCATCCA GTTTCATGTT 
TTTAGTGTCA GTGATGCTAG TGGGTGTTCA TCACCTTGCT CTCCTATGTC TAAGGATGGT 
AAATGTAGCC ATAAAAGCAG CGCCTCACCC TCACGTAAGA AGATGCAGTC CAAAAAGATT 
GAGGAGGATG TAAATAAACC AAAAGATTCA TCTACAAAGG ACTCATTACA AAGTTCTTCT 
ACAAGACGGG ATAGGATGTG CTCTCCATGT AGCCACAGAT TATCCAGGAG GAGGAGGTCA 
AGGAGTTCAG AATTATATTC ACAGCATTCT CACTACAGAG ACGGCCATGG CCAGAGAACC 
AGGAGGAAAC AAGGCAGCAA AGAGAGGTTA AAAAGTGGAC CATCAAGAAG CAGAGACAGT 
AGCAGACATA GGAGTAAAAA AGACAAGAGG AGATCAAGAC CTCGAGACAT AAGTTTATCT 
AGAAAAAGCC AGACCATGTC TCTAAGTAGT GAGAGCACTA TATCTAGAGA TGTAAGCAGG 
TCCCGGTCCC GGAGTAATGA CCATAGCAGA AGAAGATCAA GGAGCAGAGA TAGCGATCAT 
TATTATATGA GAGATAATTA CCAAAGTAGA TACCAGTGGG AATACACTTT TTACAGTAGA 
AACATGGACA GAGATGGTTA TGAATCCTCA TACAGGAGGA GAACTGAGGC AAGAGCTCAT 
TATTCAAGGC AATCTGCTAG TCCAGAGTAC AGAATACAAT CGTTTTCTGA AAGGACAAAT 
ACTCGAAATC AGAGAGGTCA CAATGAAAGC CGGTATTATT ACTATGAAAG ACACCGATCA 
AGGAGTCGAT CCAGTAATAG ATCTCGGACT ACTTCTGATA GAATGCGATG TGAAAAGCCT 
GGTGGAAAAA GGAAATACAA AACTCGCCAC TTGGAGAATG CACACAAGGA GAATGAGACA 
CGTTCGTGTG AAGTGAAAGG AAGTGACCTC CAGAAGCCTT TGTCAAAATA TAGTGGTTGC 
TACAAAAATG AGGACAGCAT TTTAGACAAT CAAGCCAGTG TTGGGACTAG GCATAAAAAG 
AGGAAGAAGA AGACAAGAAG TCCAAGTGTA GAGATTATTT ATGAAGGAAG AGCGACTGAT 
ACAACCAGAC ATCATAAAAA GAAAAAGAAA AAGCACAAGA AGAAACACAG GCGACATCAT 
ATGGGTAACT CGTCACATTC TTCTCCAGTT GTGATTACAA TTGACAGTGA TAGTGATAAG 
GAGACTGCAA TACAAGAAAA CACTGAATGT GACAGTAATA TCTCTTGGAC AACTACAACC 
CAGATATATG AGAGAGATAA TGAGACTCCA TCCCCTGTCC TGGGAACAAG TGACTGTAAG 
GATGAGGACA CAGTTGCTGG TGAAGGTGGA TTGCTGGACA AGGATTCTTG TATTAATATC 
ACTAGGGGGG ACTTAGCTGA TGAAATTGGA AATGTAGGTT GCCAATTTCA GGAAATATCA 
AATGAGCAGG CTCTTGCAGT GGCAGATGGT GGTAGTGCAT CTGACATGGG AACTCCTCTG 
AGCAATGTTG AAATTATACA AACTGAATCA AGTCAGTTGC CTTCTTCCAG AGCATCATTT 
GTAGGGAATT CGGAGAGACC TCCTTTGATA CTAAGGCTGC CAAAGAGACT TATTGACAGA 
TCCTATTGGC TTGACTCCCC AGAAAAAAAG ATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006118046.1
CDS	55..3090
Translation	MISSSEEFTVDSSFSPKAGTSKLHQTVPTDTSPDSKCPICLDRFDNVAYLDRCLHRFCFRCVQEWSKNKAECPLCKQPFHSIFHSVRAEDDFKEYVLRPLQNGSFASPDGRRFRYRTTLTRERHTPAYPRRSSSSRRTVSPPDNGILFEGLSSQPVRQRDGGIHQMIRRLASRRQASAEGRSLRQIQEQDIINFRRALYRSGVRVRNIQDGGRYRDISAEFFRRNPACLHRLVPWLKRELTVLFGAHGSLVNIVQHIIMSNVTRYDLESQAFADDLKPFLLHRTNHFLHEFISFARCPFNIEAYDQHANYDCPAPSYEEGSQSESSVITISPDETDSQEPDHNASTTSVGRAPWDDETPGPSYSTSEQVHVAMASPLDTSETSDEEPTRNGTELQGQLQDNTAPNGESDSCSDNCVIVGYVKPLAERTPELVELSSDSEESVDDGKSEDVKKSQPIQFHVFSVSDASGCSSPCSPMSKDGKCSHKSSASPSRKKMQSKKIEEDVNKPKDSSTKDSLQSSSTRRDRMCSPCSHRLSRRRRSRSSELYSQHSHYRDGHGQRTRRKQGSKERLKSGPSRSRDSSRHRSKKDKRRSRPRDISLSRKSQTMSLSSESTISRDVSRSRSRSNDHSRRRSRSRDSDHYYMRDNYQSRYQWEYTFYSRNMDRDGYESSYRRRTEARAHYSRQSASPEYRIQSFSERTNTRNQRGHNESRYYYYERHRSRSRSSNRSRTTSDRMRCEKPGGKRKYKTRHLENAHKENETRSCEVKGSDLQKPLSKYSGCYKNEDSILDNQASVGTRHKKRKKKTRSPSVEIIYEGRATDTTRHHKKKKKKHKKKHRRHHMGNSSHSSPVVITIDSDSDKETAIQENTECDSNISWTTTTQIYERDNETPSPVLGTSDCKDEDTVAGEGGLLDKDSCINITRGDLADEIGNVGCQFQEISNEQALAVADGGSASDMGTPLSNVEIIQTESSQLPSSRASFVGNSERPPLILRLPKRLIDRSYWLDSPEKKM

Target ORF information:

RefSeq Version	XM_006117984.2
Organism	Pelodiscus sinensis(Chinese soft-shelled turtle)
Definition	Pelodiscus sinensis topoisomerase I binding, arginine/serine-rich, E3 ubiquitin protein ligase (TOPORS), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006117984.2

ORF Insert Sequence:

ATGATATCAT CAAGTGAGGA GTTTACAGTG GATAGCAGTT TTTCACCGAA AGCTGGCACA 
AGCAAGCTGC ATCAGACAGT GCCAACAGAT ACATCTCCAG ATTCTAAATG TCCAATCTGC 
CTGGACAGAT TTGACAACGT GGCCTACTTA GATCGTTGCT TGCATAGGTT CTGCTTTCGT 
TGTGTACAGG AATGGTCAAA AAACAAAGCT GAATGTCCAC TCTGCAAACA ACCTTTCCAT 
TCTATCTTCC ATTCAGTGAG AGCTGAAGAT GATTTCAAGG AGTATGTACT CAGGCCTTTA 
CAGAATGGCT CTTTTGCTAG TCCTGATGGG CGGAGATTTC GTTACCGTAC TACGTTAACA 
AGGGAACGGC ATACACCAGC TTATCCTCGA AGAAGTTCCT CTTCTCGAAG GACAGTGTCA 
CCTCCAGATA ATGGGATATT ATTTGAAGGA TTATCCAGTC AACCAGTACG ACAAAGAGAT 
GGAGGAATTC ATCAGATGAT TAGACGGCTT GCATCAAGGA GACAAGCTAG TGCAGAGGGT 
AGGTCGCTGC GGCAAATTCA AGAACAAGAT ATAATTAATT TCCGAAGAGC TCTGTATCGT 
TCTGGTGTGC GTGTTAGAAA TATTCAAGAT GGTGGTCGTT ACAGAGACAT TTCAGCTGAG 
TTTTTCCGCA GGAACCCTGC CTGTCTTCAC AGATTAGTTC CATGGCTAAA GCGTGAGTTA 
ACTGTCCTGT TTGGTGCCCA TGGATCTCTA GTCAATATTG TACAGCATAT CATTATGAGT 
AATGTGACTA GATACGATTT GGAAAGTCAG GCCTTTGCTG ATGACTTAAA GCCTTTCTTG 
CTCCATCGTA CTAACCACTT TTTACATGAA TTCATCAGCT TTGCCCGATG TCCTTTTAAC 
ATAGAGGCAT ATGACCAGCA TGCCAATTAT GATTGTCCTG CTCCTTCATA TGAAGAAGGA 
AGCCAGTCGG AATCATCTGT TATTACGATA TCTCCAGATG AAACAGATTC TCAAGAACCA 
GATCATAATG CATCCACGAC TAGTGTTGGT CGAGCACCCT GGGATGATGA AACTCCAGGG 
CCATCGTATT CCACCTCAGA GCAGGTTCAT GTTGCCATGG CTTCTCCTTT GGATACTTCA 
GAAACTTCTG ATGAAGAACC CACTAGAAAT GGAACTGAGC TGCAAGGGCA GTTACAAGAT 
AATACAGCCC CAAATGGTGA GAGTGATTCT TGTTCAGACA ACTGCGTCAT TGTTGGGTAT 
GTTAAGCCCT TAGCTGAAAG AACACCAGAA CTTGTTGAGC TGTCCTCAGA CTCTGAAGAG 
TCAGTTGATG ATGGGAAAAG CGAGGATGTG AAGAAATCAC AGCCCATCCA GTTTCATGTT 
TTTAGTGTCA GTGATGCTAG TGGGTGTTCA TCACCTTGCT CTCCTATGTC TAAGGATGGT 
AAATGTAGCC ATAAAAGCAG CGCCTCACCC TCACGTAAGA AGATGCAGTC CAAAAAGATT 
GAGGAGGATG TAAATAAACC AAAAGATTCA TCTACAAAGG ACTCATTACA AAGTTCTTCT 
ACAAGACGGG ATAGGATGTG CTCTCCATGT AGCCACAGAT TATCCAGGAG GAGGAGGTCA 
AGGAGTTCAG AATTATATTC ACAGCATTCT CACTACAGAG ACGGCCATGG CCAGAGAACC 
AGGAGGAAAC AAGGCAGCAA AGAGAGGTTA AAAAGTGGAC CATCAAGAAG CAGAGACAGT 
AGCAGACATA GGAGTAAAAA AGACAAGAGG AGATCAAGAC CTCGAGACAT AAGTTTATCT 
AGAAAAAGCC AGACCATGTC TCTAAGTAGT GAGAGCACTA TATCTAGAGA TGTAAGCAGG 
TCCCGGTCCC GGAGTAATGA CCATAGCAGA AGAAGATCAA GGAGCAGAGA TAGCGATCAT 
TATTATATGA GAGATAATTA CCAAAGTAGA TACCAGTGGG AATACACTTT TTACAGTAGA 
AACATGGACA GAGATGGTTA TGAATCCTCA TACAGGAGGA GAACTGAGGC AAGAGCTCAT 
TATTCAAGGC AATCTGCTAG TCCAGAGTAC AGAATACAAT CGTTTTCTGA AAGGACAAAT 
ACTCGAAATC AGAGAGGTCA CAATGAAAGC CGGTATTATT ACTATGAAAG ACACCGATCA 
AGGAGTCGAT CCAGTAATAG ATCTCGGACT ACTTCTGATA GAATGCGATG TGAAAAGCCT 
GGTGGAAAAA GGAAATACAA AACTCGCCAC TTGGAGAATG CACACAAGGA GAATGAGACA 
CGTTCGTGTG AAGTGAAAGG AAGTGACCTC CAGAAGCCTT TGTCAAAATA TAGTGGTTGC 
TACAAAAATG AGGACAGCAT TTTAGACAAT CAAGCCAGTG TTGGGACTAG GCATAAAAAG 
AGGAAGAAGA AGACAAGAAG TCCAAGTGTA GAGATTATTT ATGAAGGAAG AGCGACTGAT 
ACAACCAGAC ATCATAAAAA GAAAAAGAAA AAGCACAAGA AGAAACACAG GCGACATCAT 
ATGGGTAACT CGTCACATTC TTCTCCAGTT GTGATTACAA TTGACAGTGA TAGTGATAAG 
GAGACTGCAA TACAAGAAAA CACTGAATGT GACAGTAATA TCTCTTGGAC AACTACAACC 
CAGATATATG AGAGAGATAA TGAGACTCCA TCCCCTGTCC TGGGAACAAG TGACTGTAAG 
GATGAGGACA CAGTTGCTGG TGAAGGTGGA TTGCTGGACA AGGATTCTTG TATTAATATC 
ACTAGGGGGG ACTTAGCTGA TGAAATTGGA AATGTAGGTT GCCAATTTCA GGAAATATCA 
AATGAGCAGG CTCTTGCAGT GGCAGATGGT GGTAGTGCAT CTGACATGGG AACTCCTCTG 
AGCAATGTTG AAATTATACA AACTGAATCA AGTCAGTTGC CTTCTTCCAG AGCATCATTT 
GTAGGGAATT CGGAGAGACC TCCTTTGATA CTAAGGCTGC CAAAGAGACT TATTGACAGA 
TCCTATTGGC TTGACTCCCC AGAAAAAAAG ATGTAA