WLS cDNA ORF clone, Parus major(Great Tit)

Gene
Clones
gRNAs

Summary

Gene Symbol	WLS
Entrez Gene ID	107208444
Full Name	wntless Wnt ligand secretion mediator
Gene Type	protein-coding
Organism	Parus major(Great Tit)

mRNA and Protein(s)

mRNA	Protein	Name
XM_015636861.2	XP_015492347.1	protein wntless homolog

Pathways from BioSystems
Gene Ontology

The following WLS gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the WLS cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OPa183740	XM_015636861.2 Latest version!	Parus major wntless Wnt ligand secretion mediator (WLS), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

WLS ( XM_015636861.2 ) cDNA ORF clone, Parus major(Great Tit) -> XP_015492347.1 Parus major wntless Wnt ligand secretion mediator (WLS), mRNA.

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CloneID	OPa183740
Clone ID Related Accession (Same CDS sequence)	XM_015636861.2
Accession Version	XM_015636861.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1626bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-11-13
Organism	Parus major(Great Tit)
Product	protein wntless homolog
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_031777.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Nov 4, 2016 this sequence version replaced XM_015636861.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Parus major Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGGGG CCATCATCGA GAACATGAGC ACCCGCAAGC TCTGCATCGT CGGGGGCATC 
CTGCTCGTGT TCCAAGTCAT CGCCTTCCTG GTGGGAGGGC TCATCGCTCC CAGCCCCACG 
ACAGCCGTTC CCTACACGTC AGTGAAGTGC ATTGATGTGA GGAAGAACCA CCACAAAACC 
AAGTGGCTGA TGCCCTGGGG ACCCAACCAC TGCAAGAAGC TGAAGGACTT TGATGAGGCC 
GTGAGCCAGC AGATCGAGGC CAACGACATC GTGTTCGCCG TTCACATCCC GCTGCCCAGC 
AAGGAGATGA GCCCCTGGTT CCAGTTCATG CTCTTCATCA TGCAGCTGGA CATCGCCTTC 
AAGATGGACA ACGACCTCAA AGAGAATGCA GAGATCACCC TGGATTTGTC GCTGGCCTAT 
CGTGATGACA TGTTTGATGA CTGGGAGGAA ATAGCACATG CCATAGAGAT CAGGAAGCTG 
AAGTGCACCT TTGGCTCTCC AAAAACGGTG GAGTCCGAGG GCCGTCACTA TGACTGTGAC 
TTCCTGCCCT TCATGGAGAT TGGCAGCGTG GCACACAAGT ACTACCTCAT CAACATCCGC 
CTGCCCGTCA ACGACAGGAA GGGCATCAAC GTGGGCATCG GTGAGATCAA GGACATCCGC 
CTCGTGGGCA TCCACCAAAA CGGAGGCTTC ACCAAGGTGT GGTTTGCCAT GAAGACCTTC 
CTGACTCCCA GCATTCTGAT CATCATGGTG TGGTACTGGA GGAGGATCAC GCTGATGACA 
CGTGCTCCCG TCCTGCTGGA GAAGGTCATC TTTGCTCTGG GAATTTCCAT GACGTTCATT 
AACATCCCTG TGGAGTGGTT TTCCATCGGA TTTGACTGGA CTTGGATGCT GCTCTTTGGA 
GACATTCGGC AGGGCATCTT CTATGCCATG CTGCTCTCGT TTTGGATCAT CTTCTGCGGG 
GAGCACATGA TGGACCAGAA TGAGCGGAAT CGGCTCTCGG GGTACTGGAA GCAGGTTGGA 
CCCATCGCCG TGGGCTCCTT CTGCCTCTTC ATCTTCGACA TGTGCGAGAG GGGAGTGCAG 
CTCAAGAACC CCTTCTACAG CATCTGGACC ACCGAGGTGG GCACGGAGCT GGCTATGGCC 
TTTATTATAG TAGCAGGGAT CTGCCTGTGC CTCTATTTTC TCTTCCTGTG TTTCATGGTC 
TTCCAAGTGT TCAGAAACAT CAGTGGGAAG CAGTCGAGCC TCCCTGCCAT GAGCAAGGCT 
CGCCGCCTTC ACTACGAGGG GCTGATTTTT AGGTTCAAGT TCCTGATGCT CATCACCCTG 
GCCTGTGCAG CCATGACCGT CATCTTCTTC ATCGTGAGCC AGGTGACAGA AGGCCACTGG 
AAGTGGGGGG AAGTCACAAT CCAGGTCAAC AGTGCCTTCT TCACCGGCAT CTACGGGATG 
TGGAATCTCT ACGTGTTCGC CCTCATGTTC CTGTACGCAC CATCCCACAA GAACTACGGT 
GAAGACCAGT CCAATGGTGA CCTGGGGGTG AACAGCGGGG AGGAGCTGCA GCTCACCACC 
ACCATCACCC ACGTGGACGG GCCCACAGAG GTCTACAAGC TGGCTCGGAA GGAGGCTCAG 
GAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015492347.1
CDS	79..1704
Translation	MAGAIIENMSTRKLCIVGGILLVFQVIAFLVGGLIAPSPTTAVPYTSVKCIDVRKNHHKTKWLMPWGPNHCKKLKDFDEAVSQQIEANDIVFAVHIPLPSKEMSPWFQFMLFIMQLDIAFKMDNDLKENAEITLDLSLAYRDDMFDDWEEIAHAIEIRKLKCTFGSPKTVESEGRHYDCDFLPFMEIGSVAHKYYLINIRLPVNDRKGINVGIGEIKDIRLVGIHQNGGFTKVWFAMKTFLTPSILIIMVWYWRRITLMTRAPVLLEKVIFALGISMTFINIPVEWFSIGFDWTWMLLFGDIRQGIFYAMLLSFWIIFCGEHMMDQNERNRLSGYWKQVGPIAVGSFCLFIFDMCERGVQLKNPFYSIWTTEVGTELAMAFIIVAGICLCLYFLFLCFMVFQVFRNISGKQSSLPAMSKARRLHYEGLIFRFKFLMLITLACAAMTVIFFIVSQVTEGHWKWGEVTIQVNSAFFTGIYGMWNLYVFALMFLYAPSHKNYGEDQSNGDLGVNSGEELQLTTTITHVDGPTEVYKLARKEAQE

Target ORF information:

RefSeq Version	XM_015636861.2
Organism	Parus major(Great Tit)
Definition	Parus major wntless Wnt ligand secretion mediator (WLS), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015636861.2

ORF Insert Sequence:

ATGGCCGGGG CCATCATCGA GAACATGAGC ACCCGCAAGC TCTGCATCGT CGGGGGCATC 
CTGCTCGTGT TCCAAGTCAT CGCCTTCCTG GTGGGAGGGC TCATCGCTCC CAGCCCCACG 
ACAGCCGTTC CCTACACGTC AGTGAAGTGC ATTGATGTGA GGAAGAACCA CCACAAAACC 
AAGTGGCTGA TGCCCTGGGG ACCCAACCAC TGCAAGAAGC TGAAGGACTT TGATGAGGCC 
GTGAGCCAGC AGATCGAGGC CAACGACATC GTGTTCGCCG TTCACATCCC GCTGCCCAGC 
AAGGAGATGA GCCCCTGGTT CCAGTTCATG CTCTTCATCA TGCAGCTGGA CATCGCCTTC 
AAGATGGACA ACGACCTCAA AGAGAATGCA GAGATCACCC TGGATTTGTC GCTGGCCTAT 
CGTGATGACA TGTTTGATGA CTGGGAGGAA ATAGCACATG CCATAGAGAT CAGGAAGCTG 
AAGTGCACCT TTGGCTCTCC AAAAACGGTG GAGTCCGAGG GCCGTCACTA TGACTGTGAC 
TTCCTGCCCT TCATGGAGAT TGGCAGCGTG GCACACAAGT ACTACCTCAT CAACATCCGC 
CTGCCCGTCA ACGACAGGAA GGGCATCAAC GTGGGCATCG GTGAGATCAA GGACATCCGC 
CTCGTGGGCA TCCACCAAAA CGGAGGCTTC ACCAAGGTGT GGTTTGCCAT GAAGACCTTC 
CTGACTCCCA GCATTCTGAT CATCATGGTG TGGTACTGGA GGAGGATCAC GCTGATGACA 
CGTGCTCCCG TCCTGCTGGA GAAGGTCATC TTTGCTCTGG GAATTTCCAT GACGTTCATT 
AACATCCCTG TGGAGTGGTT TTCCATCGGA TTTGACTGGA CTTGGATGCT GCTCTTTGGA 
GACATTCGGC AGGGCATCTT CTATGCCATG CTGCTCTCGT TTTGGATCAT CTTCTGCGGG 
GAGCACATGA TGGACCAGAA TGAGCGGAAT CGGCTCTCGG GGTACTGGAA GCAGGTTGGA 
CCCATCGCCG TGGGCTCCTT CTGCCTCTTC ATCTTCGACA TGTGCGAGAG GGGAGTGCAG 
CTCAAGAACC CCTTCTACAG CATCTGGACC ACCGAGGTGG GCACGGAGCT GGCTATGGCC 
TTTATTATAG TAGCAGGGAT CTGCCTGTGC CTCTATTTTC TCTTCCTGTG TTTCATGGTC 
TTCCAAGTGT TCAGAAACAT CAGTGGGAAG CAGTCGAGCC TCCCTGCCAT GAGCAAGGCT 
CGCCGCCTTC ACTACGAGGG GCTGATTTTT AGGTTCAAGT TCCTGATGCT CATCACCCTG 
GCCTGTGCAG CCATGACCGT CATCTTCTTC ATCGTGAGCC AGGTGACAGA AGGCCACTGG 
AAGTGGGGGG AAGTCACAAT CCAGGTCAAC AGTGCCTTCT TCACCGGCAT CTACGGGATG 
TGGAATCTCT ACGTGTTCGC CCTCATGTTC CTGTACGCAC CATCCCACAA GAACTACGGT 
GAAGACCAGT CCAATGGTGA CCTGGGGGTG AACAGCGGGG AGGAGCTGCA GCTCACCACC 
ACCATCACCC ACGTGGACGG GCCCACAGAG GTCTACAAGC TGGCTCGGAA GGAGGCTCAG 
GAGTGA