EGFR cDNA ORF clone, Parus major(Great Tit)

Gene
Clones
gRNAs

Summary

Gene Symbol	EGFR
Entrez Gene ID	107200328
Full Name	epidermal growth factor receptor
Gene Type	protein-coding
Organism	Parus major(Great Tit)

mRNA and Protein(s)

mRNA	Protein	Name
XM_015618676.2	XP_015474162.1	epidermal growth factor receptor isoform X2
XM_015618675.2	XP_015474161.1	epidermal growth factor receptor isoform X1

Pathways from BioSystems
Gene Ontology

The following EGFR gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the EGFR cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OPa168421	XM_015618675.2 Latest version!	Parus major epidermal growth factor receptor (EGFR), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00
OPa168422	XM_015618676.2 Latest version!	Parus major epidermal growth factor receptor (EGFR), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

EGFR ( XM_015618675.2 ) cDNA ORF clone, Parus major(Great Tit) -> XP_015474161.1 Parus major epidermal growth factor receptor (EGFR), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OPa168421
Clone ID Related Accession (Same CDS sequence)	XM_015618675.2
Accession Version	XM_015618675.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3672bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-11-13
Organism	Parus major(Great Tit)
Product	epidermal growth factor receptor isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_031769.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Nov 4, 2016 this sequence version replaced XM_015618675.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Parus major Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCGGCC GCAGCCCCCT CGCTGCCGCC GGGCCCCGCG GCGCCGCTGT CCTGGTGCTG 
CTGCTGGGGC GCCTCGCTCT CTGCCTCGCC GTGGAGGGAA AGAAAGTTTG TCAAGGGACG 
AATAACAAGC TGACCCAGCT GGGACATGTG GAAGACCATT TCACCAGCCT GCAGAGGATG 
TACAATAATT GTGAGGTGGT ACTCAGCAAC CTGGAGATTA CATATGTGGA GCACAATCGT 
GACCTCTCTT TCCTGAAGAC AATACAGGAG GTTGCAGGCT ACGTGCTCAT CGCACTTAAC 
ATGGTGGATG TCATTCCCCT TGAGAACCTC CAGATCATCC GAGGCAACGT GCTGTATGAC 
AACTCTTACG CCCTGGCAGT TTTATCCAAC TACCACATGA ATAAAACCCA GGGGCTCCGG 
CAGCTGCCAA TGAAGCGGCT TTCAGAAATT CTCAATGGAG GTGTTAAAAT CAGCAACAAC 
CCCAAACTGT GCAACATGGA CACTGTTCTT TGGAATGACA TCATTGACAC AAGCAAGAAG 
CCTCCCACAG TGCTTGAATT TGCGAGCAAC CTATCTTCTT GTCCCAAATG CCATCAGAAC 
TGCACAGAAG ACCACTGCTG GGGCCCAGGC CAGCAAAACT GCCAGACATT AACAAAAGTC 
ATCTGTGCTC AGCAGTGCTC TGGTCGGTGC AGGGGAAAGG TGCCCAGCGA CTGCTGCCAC 
AACCAGTGTG CTGCGGGGTG CACCGGCCCT CGGGAGAGTG ACTGCCTGGC ATGCCGCAAG 
TTTCGGGATG ATGCTACGTG CAAGGATACA TGTCCTCCAT TGGTCCTGTA TAACCCCACC 
ACTTACCAGA TGGATGTGAA CCCTGAAGGA AAATACAGCT TTGGAGCCAC TTGTGTGAAG 
GAATGTCCAC ACAACTATGT GGTGACAGAC CATGGCTCCT GCGTTCGCTC GTGCAATGCT 
GATACTTACG AAGTTGAAGA AAATGGTGTT CGGAAATGTA AAAAGTGTGA TGGGCTATGC 
AGCAAAGTGT GCAATGGCAT CGGAATAGGT GAGCTTAAAG GGATCCTCTC CATAAATGCC 
ACAAATATCG ACTCCTTCAA AAACTGTACC AAGATCAATG GGGATGTCAG CATTCTCCCA 
GTTGCATTTC TAGGTGATGC CTTCACGAAG ACCCTACCTT TGGATCCCAA GAAGTTGGAT 
GTCTTCAAAA CAGTCAAAGA AATATCAGGA TTTTTGTTGA TTCAAGCCTG GCCTGAGAAT 
GCTACTGATC TCTATGCTTT TGAAAATCTG GAGATTATAC GAGGCAGAAC AAAGCAACAT 
GGCCAGTATT CCCTTGCCGT TGTTAACTTG AAAATAAAGT CCCTAGGGCT GCGGTCCCTC 
AAGGAAATAA GTGATGGAGA TGTTGCCATT ATGAGGAACA AGAACCTCTG CTATGCTGAC 
ACCATGGACT GGCACAGCCT GTTTGCAACT GAGAGCCAGA AAACGAAGAT TTTACAGAAT 
AGAGATAAAA ATGAGTGTGC TGCTGCCAAG CATGTTTGTG ACCCCCTGTG CTCAGATGTA 
GGCTGCTGGG GCCCAGGGCC CTTCCACTGC TTCTCCTGCA GGTTTTTTGA TCGTCAGAAG 
GAATGTGTGA AACAGTGCAA CATCCTGCAA GGGGAGCCAA GAGAGTTTGA AAAGGACTCC 
AAGTGCCTGC CCTGCCACCC TGAGTGCCTG GTGCAAAACT CCACCATGTA CAATGCGACC 
TGCACTGGAC CTGGCCCTGA CAATTGCATG AAGTGTGCCC ATTTTATAGA TGGCCCCCAC 
TGTGTGAAAT CCTGCCCAGC AGGGGTTCTG GGTGAGAATG ACACCCTCGT CTGGAAATAT 
GCCGATGGGA ATAATGTTTG CCAGCTCTGC CATCCAAACT GCACCCGGGG GTGCAAAGGG 
CCAGGTCTTG AAGGCTGTCC AAATGGCTCC AAGATCCCGT CTATCGCAGC CGGTGTTGTC 
GGAGGTCTCC TGTGCCTGGT GGTGGTGGGC CTGGGCATCG GCCTGTACCT GCGGAGACGC 
CACATCGTCA GAAAGCGGAC CCTGCGCCGG CTGCTGCAGG AGAGGGAGCT TGTTGAACCA 
CTGACACCCA GCGGGGAGGC ACCAAACCAG GCTCATCTGA GAATTTTAAA GGAAACAGAA 
TTTAAAAAGG TCAAAGTTTT AGGCTCTGGA GCTTTTGGCA CTGTTTATAA GGGACTTTGG 
ATCCCAGAAG GAGAGAAGGT TAAAATTCCT GTTGCTATTA AAGAGTTGAG AGAGGCTACA 
TCACCAAAAG CCAACAAGGA AATACTTGAT GAAGCTTATG TGATGGCTAG TGTTGACAAT 
CCTCATGTGT GTCGCTTGTT GGGCATCTGC CTCACTTCAA CTGTCCAGCT CATCACACAG 
CTGATGCCTT ACGGCTGCCT CCTGGATTAC ATCCGAGAGC ACAAGGACAA CATCGGCTCT 
CAGTACCTCC TCAACTGGTG TGTGCAGATT GCAAAGGGAA TGAACTATCT GGAGGAACGG 
CGCCTGGTGC ACCGGGACCT TGCTGCAAGG AATGTCCTCG TTAAGACTCC TCAGCATGTG 
AAAATCACGG ACTTTGGGCT GGCAAAGCTG CTCGGTGCAG ACGAGAAGGA GTATCATGCT 
GAGGGAGGCA AGGTTCCTAT TAAATGGATG GCATTGGAGT CAATTTTACA CCGGATTTAC 
ACTCATCAAA GTGATGTCTG GAGTTATGGT GTGACAGTCT GGGAGCTGAT GACATTTGGG 
TCCAAGCCAT ATGAAGGGAT CCCTGCCAGT GAGATCTCCT CTGTCCTGGA GAAGGGCGAG 
CGTTTGCCGC AGCCCCCCAT CTGTACCATC GACGTCTACA TGATCATGGT CAAATGCTGG 
ATGATCGATG CAGACAGCCG TCCCAAGTTT CGTGAGCTGA TAGCTGAATT CTCAAAAATG 
GCCCGTGACC CTCCACGCTA TCTTGTTATA CAGGGAGATG ATAGGATGCA CCTGCCCAGC 
CCTACGGACT CCAAGTTTTA CCGCACCCTG ATGGAGGAGG AGGACATGGA AGATATAGTG 
GATGCAGATG AGTACCTTGT GCCACACCAG GGCTTTTTCA ACAGCCCTTC AACATCTCGC 
ACTCCTCTCT TGAGTTCATT GAGTGCTACC AGCAACAACT CTGCTACAAC CTGCATTGAC 
AGGAACGGGC AGGGGCACCC TGTGCGGGAA GACAGCTTTG TTCAGAGGTA TAGCTCAGAC 
CCAACAGGTG CTTTCTTGGA GGACAGCATA GATGACAGCT TCCTACCAGC CCCAGAGTAT 
GTAAACCAAT TGGTGCCCAA GAAAGCATCT GCCTCAGCAG TCCAGAATCC AATCTACAAC 
AACTTCTCTC TCACAGCAAA CTCAAAGGTC CCCACAGACT CCAGCTACCA GAATTCACAC 
AGCACAGCTG TGGACAACCC CGAGTATCTC AACACCAACC AGTCTCCCCT GGCCAAGACA 
GTCTTTGAGA GCTCTCCCTA CTGGATCCAA GCGGGCAACC ACCAAATAAA TCTGGACAAT 
CCTGACTACC AGCAGGACTT CTTACCGAAG GAAACCAAAC CTAACGGTCT CTTAAAAGTT 
CCTGCAGCAG AAAACCCAGA GTACTTGAGG GTAGCGGCAC CAAAAAGTGA ATACATTGAG 
GCCTCAGCAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015474161.1
CDS	383..4054
Translation	MGGRSPLAAAGPRGAAVLVLLLGRLALCLAVEGKKVCQGTNNKLTQLGHVEDHFTSLQRMYNNCEVVLSNLEITYVEHNRDLSFLKTIQEVAGYVLIALNMVDVIPLENLQIIRGNVLYDNSYALAVLSNYHMNKTQGLRQLPMKRLSEILNGGVKISNNPKLCNMDTVLWNDIIDTSKKPPTVLEFASNLSSCPKCHQNCTEDHCWGPGQQNCQTLTKVICAQQCSGRCRGKVPSDCCHNQCAAGCTGPRESDCLACRKFRDDATCKDTCPPLVLYNPTTYQMDVNPEGKYSFGATCVKECPHNYVVTDHGSCVRSCNADTYEVEENGVRKCKKCDGLCSKVCNGIGIGELKGILSINATNIDSFKNCTKINGDVSILPVAFLGDAFTKTLPLDPKKLDVFKTVKEISGFLLIQAWPENATDLYAFENLEIIRGRTKQHGQYSLAVVNLKIKSLGLRSLKEISDGDVAIMRNKNLCYADTMDWHSLFATESQKTKILQNRDKNECAAAKHVCDPLCSDVGCWGPGPFHCFSCRFFDRQKECVKQCNILQGEPREFEKDSKCLPCHPECLVQNSTMYNATCTGPGPDNCMKCAHFIDGPHCVKSCPAGVLGENDTLVWKYADGNNVCQLCHPNCTRGCKGPGLEGCPNGSKIPSIAAGVVGGLLCLVVVGLGIGLYLRRRHIVRKRTLRRLLQERELVEPLTPSGEAPNQAHLRILKETEFKKVKVLGSGAFGTVYKGLWIPEGEKVKIPVAIKELREATSPKANKEILDEAYVMASVDNPHVCRLLGICLTSTVQLITQLMPYGCLLDYIREHKDNIGSQYLLNWCVQIAKGMNYLEERRLVHRDLAARNVLVKTPQHVKITDFGLAKLLGADEKEYHAEGGKVPIKWMALESILHRIYTHQSDVWSYGVTVWELMTFGSKPYEGIPASEISSVLEKGERLPQPPICTIDVYMIMVKCWMIDADSRPKFRELIAEFSKMARDPPRYLVIQGDDRMHLPSPTDSKFYRTLMEEEDMEDIVDADEYLVPHQGFFNSPSTSRTPLLSSLSATSNNSATTCIDRNGQGHPVREDSFVQRYSSDPTGAFLEDSIDDSFLPAPEYVNQLVPKKASASAVQNPIYNNFSLTANSKVPTDSSYQNSHSTAVDNPEYLNTNQSPLAKTVFESSPYWIQAGNHQINLDNPDYQQDFLPKETKPNGLLKVPAAENPEYLRVAAPKSEYIEASA

Target ORF information:

RefSeq Version	XM_015618675.2
Organism	Parus major(Great Tit)
Definition	Parus major epidermal growth factor receptor (EGFR), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015618675.2

ORF Insert Sequence:

ATGGGCGGCC GCAGCCCCCT CGCTGCCGCC GGGCCCCGCG GCGCCGCTGT CCTGGTGCTG 
CTGCTGGGGC GCCTCGCTCT CTGCCTCGCC GTGGAGGGAA AGAAAGTTTG TCAAGGGACG 
AATAACAAGC TGACCCAGCT GGGACATGTG GAAGACCATT TCACCAGCCT GCAGAGGATG 
TACAATAATT GTGAGGTGGT ACTCAGCAAC CTGGAGATTA CATATGTGGA GCACAATCGT 
GACCTCTCTT TCCTGAAGAC AATACAGGAG GTTGCAGGCT ACGTGCTCAT CGCACTTAAC 
ATGGTGGATG TCATTCCCCT TGAGAACCTC CAGATCATCC GAGGCAACGT GCTGTATGAC 
AACTCTTACG CCCTGGCAGT TTTATCCAAC TACCACATGA ATAAAACCCA GGGGCTCCGG 
CAGCTGCCAA TGAAGCGGCT TTCAGAAATT CTCAATGGAG GTGTTAAAAT CAGCAACAAC 
CCCAAACTGT GCAACATGGA CACTGTTCTT TGGAATGACA TCATTGACAC AAGCAAGAAG 
CCTCCCACAG TGCTTGAATT TGCGAGCAAC CTATCTTCTT GTCCCAAATG CCATCAGAAC 
TGCACAGAAG ACCACTGCTG GGGCCCAGGC CAGCAAAACT GCCAGACATT AACAAAAGTC 
ATCTGTGCTC AGCAGTGCTC TGGTCGGTGC AGGGGAAAGG TGCCCAGCGA CTGCTGCCAC 
AACCAGTGTG CTGCGGGGTG CACCGGCCCT CGGGAGAGTG ACTGCCTGGC ATGCCGCAAG 
TTTCGGGATG ATGCTACGTG CAAGGATACA TGTCCTCCAT TGGTCCTGTA TAACCCCACC 
ACTTACCAGA TGGATGTGAA CCCTGAAGGA AAATACAGCT TTGGAGCCAC TTGTGTGAAG 
GAATGTCCAC ACAACTATGT GGTGACAGAC CATGGCTCCT GCGTTCGCTC GTGCAATGCT 
GATACTTACG AAGTTGAAGA AAATGGTGTT CGGAAATGTA AAAAGTGTGA TGGGCTATGC 
AGCAAAGTGT GCAATGGCAT CGGAATAGGT GAGCTTAAAG GGATCCTCTC CATAAATGCC 
ACAAATATCG ACTCCTTCAA AAACTGTACC AAGATCAATG GGGATGTCAG CATTCTCCCA 
GTTGCATTTC TAGGTGATGC CTTCACGAAG ACCCTACCTT TGGATCCCAA GAAGTTGGAT 
GTCTTCAAAA CAGTCAAAGA AATATCAGGA TTTTTGTTGA TTCAAGCCTG GCCTGAGAAT 
GCTACTGATC TCTATGCTTT TGAAAATCTG GAGATTATAC GAGGCAGAAC AAAGCAACAT 
GGCCAGTATT CCCTTGCCGT TGTTAACTTG AAAATAAAGT CCCTAGGGCT GCGGTCCCTC 
AAGGAAATAA GTGATGGAGA TGTTGCCATT ATGAGGAACA AGAACCTCTG CTATGCTGAC 
ACCATGGACT GGCACAGCCT GTTTGCAACT GAGAGCCAGA AAACGAAGAT TTTACAGAAT 
AGAGATAAAA ATGAGTGTGC TGCTGCCAAG CATGTTTGTG ACCCCCTGTG CTCAGATGTA 
GGCTGCTGGG GCCCAGGGCC CTTCCACTGC TTCTCCTGCA GGTTTTTTGA TCGTCAGAAG 
GAATGTGTGA AACAGTGCAA CATCCTGCAA GGGGAGCCAA GAGAGTTTGA AAAGGACTCC 
AAGTGCCTGC CCTGCCACCC TGAGTGCCTG GTGCAAAACT CCACCATGTA CAATGCGACC 
TGCACTGGAC CTGGCCCTGA CAATTGCATG AAGTGTGCCC ATTTTATAGA TGGCCCCCAC 
TGTGTGAAAT CCTGCCCAGC AGGGGTTCTG GGTGAGAATG ACACCCTCGT CTGGAAATAT 
GCCGATGGGA ATAATGTTTG CCAGCTCTGC CATCCAAACT GCACCCGGGG GTGCAAAGGG 
CCAGGTCTTG AAGGCTGTCC AAATGGCTCC AAGATCCCGT CTATCGCAGC CGGTGTTGTC 
GGAGGTCTCC TGTGCCTGGT GGTGGTGGGC CTGGGCATCG GCCTGTACCT GCGGAGACGC 
CACATCGTCA GAAAGCGGAC CCTGCGCCGG CTGCTGCAGG AGAGGGAGCT TGTTGAACCA 
CTGACACCCA GCGGGGAGGC ACCAAACCAG GCTCATCTGA GAATTTTAAA GGAAACAGAA 
TTTAAAAAGG TCAAAGTTTT AGGCTCTGGA GCTTTTGGCA CTGTTTATAA GGGACTTTGG 
ATCCCAGAAG GAGAGAAGGT TAAAATTCCT GTTGCTATTA AAGAGTTGAG AGAGGCTACA 
TCACCAAAAG CCAACAAGGA AATACTTGAT GAAGCTTATG TGATGGCTAG TGTTGACAAT 
CCTCATGTGT GTCGCTTGTT GGGCATCTGC CTCACTTCAA CTGTCCAGCT CATCACACAG 
CTGATGCCTT ACGGCTGCCT CCTGGATTAC ATCCGAGAGC ACAAGGACAA CATCGGCTCT 
CAGTACCTCC TCAACTGGTG TGTGCAGATT GCAAAGGGAA TGAACTATCT GGAGGAACGG 
CGCCTGGTGC ACCGGGACCT TGCTGCAAGG AATGTCCTCG TTAAGACTCC TCAGCATGTG 
AAAATCACGG ACTTTGGGCT GGCAAAGCTG CTCGGTGCAG ACGAGAAGGA GTATCATGCT 
GAGGGAGGCA AGGTTCCTAT TAAATGGATG GCATTGGAGT CAATTTTACA CCGGATTTAC 
ACTCATCAAA GTGATGTCTG GAGTTATGGT GTGACAGTCT GGGAGCTGAT GACATTTGGG 
TCCAAGCCAT ATGAAGGGAT CCCTGCCAGT GAGATCTCCT CTGTCCTGGA GAAGGGCGAG 
CGTTTGCCGC AGCCCCCCAT CTGTACCATC GACGTCTACA TGATCATGGT CAAATGCTGG 
ATGATCGATG CAGACAGCCG TCCCAAGTTT CGTGAGCTGA TAGCTGAATT CTCAAAAATG 
GCCCGTGACC CTCCACGCTA TCTTGTTATA CAGGGAGATG ATAGGATGCA CCTGCCCAGC 
CCTACGGACT CCAAGTTTTA CCGCACCCTG ATGGAGGAGG AGGACATGGA AGATATAGTG 
GATGCAGATG AGTACCTTGT GCCACACCAG GGCTTTTTCA ACAGCCCTTC AACATCTCGC 
ACTCCTCTCT TGAGTTCATT GAGTGCTACC AGCAACAACT CTGCTACAAC CTGCATTGAC 
AGGAACGGGC AGGGGCACCC TGTGCGGGAA GACAGCTTTG TTCAGAGGTA TAGCTCAGAC 
CCAACAGGTG CTTTCTTGGA GGACAGCATA GATGACAGCT TCCTACCAGC CCCAGAGTAT 
GTAAACCAAT TGGTGCCCAA GAAAGCATCT GCCTCAGCAG TCCAGAATCC AATCTACAAC 
AACTTCTCTC TCACAGCAAA CTCAAAGGTC CCCACAGACT CCAGCTACCA GAATTCACAC 
AGCACAGCTG TGGACAACCC CGAGTATCTC AACACCAACC AGTCTCCCCT GGCCAAGACA 
GTCTTTGAGA GCTCTCCCTA CTGGATCCAA GCGGGCAACC ACCAAATAAA TCTGGACAAT 
CCTGACTACC AGCAGGACTT CTTACCGAAG GAAACCAAAC CTAACGGTCT CTTAAAAGTT 
CCTGCAGCAG AAAACCCAGA GTACTTGAGG GTAGCGGCAC CAAAAAGTGA ATACATTGAG 
GCCTCAGCAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EGFR ( XM_015618676.2 ) cDNA ORF clone, Parus major(Great Tit) -> XP_015474162.1 Parus major epidermal growth factor receptor (EGFR), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OPa168422
Clone ID Related Accession (Same CDS sequence)	XM_015618676.2
Accession Version	XM_015618676.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3669bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-11-13
Organism	Parus major(Great Tit)
Product	epidermal growth factor receptor isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_031769.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Nov 4, 2016 this sequence version replaced XM_015618676.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Parus major Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCGGCC GCAGCCCCCT CGCTGCCGCC GGGCCCCGCG GCGCCGCTGT CCTGGTGCTG 
CTGCTGGGGC GCCTCGCTCT CTGCCTCGCC GTGGAGGGAA AGAAAGTTTG TCAAGGGACG 
AATAACAAGC TGACCCAGCT GGGACATGTG GAAGACCATT TCACCAGCCT GCAGAGGATG 
TACAATAATT GTGAGGTGGT ACTCAGCAAC CTGGAGATTA CATATGTGGA GCACAATCGT 
GACCTCTCTT TCCTGAAGAC AATACAGGAG GTTGCAGGCT ACGTGCTCAT CGCACTTAAC 
ATGGTGGATG TCATTCCCCT TGAGAACCTC CAGATCATCC GAGGCAACGT GCTGTATGAC 
AACTCTTACG CCCTGGCAGT TTTATCCAAC TACCACATGA ATAAAACCCA GGGGCTCCGG 
CAGCTGCCAA TGAAGCGGCT TTCAGAAATT CTCAATGGAG GTGTTAAAAT CAGCAACAAC 
CCCAAACTGT GCAACATGGA CACTGTTCTT TGGAATGACA TCATTGACAC AAGCAAGAAG 
CCTCCCACAG TGCTTGAATT TGCGAGCAAC CTATCTTCTT GTCCCAAATG CCATCAGAAC 
TGCACAGAAG ACCACTGCTG GGGCCCAGGC CAGCAAAACT GCCAGACATT AACAAAAGTC 
ATCTGTGCTC AGCAGTGCTC TGGTCGGTGC AGGGGAAAGG TGCCCAGCGA CTGCTGCCAC 
AACCAGTGTG CTGCGGGGTG CACCGGCCCT CGGGAGAGTG ACTGCCTGGC ATGCCGCAAG 
TTTCGGGATG ATGCTACGTG CAAGGATACA TGTCCTCCAT TGGTCCTGTA TAACCCCACC 
ACTTACCAGA TGGATGTGAA CCCTGAAGGA AAATACAGCT TTGGAGCCAC TTGTGTGAAG 
GAATGTCCAC ACAACTATGT GGTGACAGAC CATGGCTCCT GCGTTCGCTC GTGCAATGCT 
GATACTTACG AAGTTGAAGA AAATGGTGTT CGGAAATGTA AAAAGTGTGA TGGGCTATGC 
AGCAAAGTGT GCAATGGCAT CGGAATAGGT GAGCTTAAAG GGATCCTCTC CATAAATGCC 
ACAAATATCG ACTCCTTCAA AAACTGTACC AAGATCAATG GGGATGTCAG CATTCTCCCA 
GTTGCATTTC TAGGTGATGC CTTCACGAAG ACCCTACCTT TGGATCCCAA GAAGTTGGAT 
GTCTTCAAAA CAGTCAAAGA AATATCAGGA TTTTTGTTGA TTCAAGCCTG GCCTGAGAAT 
GCTACTGATC TCTATGCTTT TGAAAATCTG GAGATTATAC GAGGCAGAAC AAAGCAACAT 
GGCCAGTATT CCCTTGCCGT TGTTAACTTG AAAATAAAGT CCCTAGGGCT GCGGTCCCTC 
AAGGAAATAA GTGATGGAGA TGTTGCCATT ATGAGGAACA AGAACCTCTG CTATGCTGAC 
ACCATGGACT GGCACAGCCT GTTTGCAACT GAGAGCCAGA AAACGAAGAT TTTACAGAAT 
AGAGATAAAA ATGAGTGTGC TGCTGCCAAG CATGTTTGTG ACCCCCTGTG CTCAGATGTA 
GGCTGCTGGG GCCCAGGGCC CTTCCACTGC TTCTCCTGCA GGTTTTTTGA TCGTCAGAAG 
GAATGTGTGA AACAGTGCAA CATCCTGCAA GGGGAGCCAA GAGAGTTTGA AAAGGACTCC 
AAGTGCCTGC CCTGCCACCC TGAGTGCCTG GTGCAAAACT CCACCATGTA CAATGCGACC 
TGCACTGGAC CTGGCCCTGA CAATTGCATG AAGTGTGCCC ATTTTATAGA TGGCCCCCAC 
TGTGTGAAAT CCTGCCCAGC AGGGGTTCTG GGTGAGAATG ACACCCTCGT CTGGAAATAT 
GCCGATGGGA ATAATGTTTG CCAGCTCTGC CATCCAAACT GCACCCGGGG GTGCAAAGGG 
CCAGGTCTTG AAGGCTGTCC AAATGGCTCC AAGATCCCGT CTATCGCAGC CGGTGTTGTC 
GGAGGTCTCC TGTGCCTGGT GGTGGTGGGC CTGGGCATCG GCCTGTACCT GCGGAGACGC 
CACATCGTCA GAAAGCGGAC CCTGCGCCGG CTGCTGCAGG AGAGGGAGCT TGTTGAACCA 
CTGACACCCA GCGGGGAGGC ACCAAACCAG GCTCATCTGA GAATTTTAAA GGAAACAGAA 
TTTAAAAAGG TCAAAGTTTT AGGCTCTGGA GCTTTTGGCA CTGTTTATAA GGGACTTTGG 
ATCCCAGAAG GAGAGAAGGT TAAAATTCCT GTTGCTATTA AAGAGTTGAG AGAGGCTACA 
TCACCAAAAG CCAACAAGGA AATACTTGAT GAAGCTTATG TGATGGCTAG TGTTGACAAT 
CCTCATGTGT GTCGCTTGTT GGGCATCTGC CTCACTTCAA CTGTCCAGCT CATCACACAG 
CTGATGCCTT ACGGCTGCCT CCTGGATTAC ATCCGAGAGC ACAAGGACAA CATCGGCTCT 
CAGTACCTCC TCAACTGGTG TGTGCAGATT GCAAAGGGAA TGAACTATCT GGAGGAACGG 
CGCCTGGTGC ACCGGGACCT TGCTGCAAGG AATGTCCTCG TTAAGACTCC TCAGCATGTG 
AAAATCACGG ACTTTGGGCT GGCAAAGCTG CTCGGTGCAG ACGAGAAGGA GTATCATGCT 
GAGGGAGGCA AGGTTCCTAT TAAATGGATG GCATTGGAGT CAATTTTACA CCGGATTTAC 
ACTCATCAAA GTGATGTCTG GAGTTATGGT GTGACAGTCT GGGAGCTGAT GACATTTGGG 
TCCAAGCCAT ATGAAGGGAT CCCTGCCAGT GAGATCTCCT CTGTCCTGGA GAAGGGCGAG 
CGTTTGCCGC AGCCCCCCAT CTGTACCATC GACGTCTACA TGATCATGGT CAAATGCTGG 
ATGATCGATG CAGACAGCCG TCCCAAGTTT CGTGAGCTGA TAGCTGAATT CTCAAAAATG 
GCCCGTGACC CTCCACGCTA TCTTGTTATA CAGGGAGATG ATAGGATGCA CCTGCCCAGC 
CCTACGGACT CCAAGTTTTA CCGCACCCTG ATGGAGGAGG AGGACATGGA AGATATAGTG 
GATGCAGATG AGTACCTTGT GCCACACCAG GGCTTTTTCA ACAGCCCTTC AACATCTCGC 
ACTCCTCTCT TGAGTTCATT GAGTGCTACC AGCAACAACT CTGCTACAAC CTGCATTGAC 
AGGAACGGGG GGCACCCTGT GCGGGAAGAC AGCTTTGTTC AGAGGTATAG CTCAGACCCA 
ACAGGTGCTT TCTTGGAGGA CAGCATAGAT GACAGCTTCC TACCAGCCCC AGAGTATGTA 
AACCAATTGG TGCCCAAGAA AGCATCTGCC TCAGCAGTCC AGAATCCAAT CTACAACAAC 
TTCTCTCTCA CAGCAAACTC AAAGGTCCCC ACAGACTCCA GCTACCAGAA TTCACACAGC 
ACAGCTGTGG ACAACCCCGA GTATCTCAAC ACCAACCAGT CTCCCCTGGC CAAGACAGTC 
TTTGAGAGCT CTCCCTACTG GATCCAAGCG GGCAACCACC AAATAAATCT GGACAATCCT 
GACTACCAGC AGGACTTCTT ACCGAAGGAA ACCAAACCTA ACGGTCTCTT AAAAGTTCCT 
GCAGCAGAAA ACCCAGAGTA CTTGAGGGTA GCGGCACCAA AAAGTGAATA CATTGAGGCC 
TCAGCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015474162.1
CDS	384..4052
Translation	MGGRSPLAAAGPRGAAVLVLLLGRLALCLAVEGKKVCQGTNNKLTQLGHVEDHFTSLQRMYNNCEVVLSNLEITYVEHNRDLSFLKTIQEVAGYVLIALNMVDVIPLENLQIIRGNVLYDNSYALAVLSNYHMNKTQGLRQLPMKRLSEILNGGVKISNNPKLCNMDTVLWNDIIDTSKKPPTVLEFASNLSSCPKCHQNCTEDHCWGPGQQNCQTLTKVICAQQCSGRCRGKVPSDCCHNQCAAGCTGPRESDCLACRKFRDDATCKDTCPPLVLYNPTTYQMDVNPEGKYSFGATCVKECPHNYVVTDHGSCVRSCNADTYEVEENGVRKCKKCDGLCSKVCNGIGIGELKGILSINATNIDSFKNCTKINGDVSILPVAFLGDAFTKTLPLDPKKLDVFKTVKEISGFLLIQAWPENATDLYAFENLEIIRGRTKQHGQYSLAVVNLKIKSLGLRSLKEISDGDVAIMRNKNLCYADTMDWHSLFATESQKTKILQNRDKNECAAAKHVCDPLCSDVGCWGPGPFHCFSCRFFDRQKECVKQCNILQGEPREFEKDSKCLPCHPECLVQNSTMYNATCTGPGPDNCMKCAHFIDGPHCVKSCPAGVLGENDTLVWKYADGNNVCQLCHPNCTRGCKGPGLEGCPNGSKIPSIAAGVVGGLLCLVVVGLGIGLYLRRRHIVRKRTLRRLLQERELVEPLTPSGEAPNQAHLRILKETEFKKVKVLGSGAFGTVYKGLWIPEGEKVKIPVAIKELREATSPKANKEILDEAYVMASVDNPHVCRLLGICLTSTVQLITQLMPYGCLLDYIREHKDNIGSQYLLNWCVQIAKGMNYLEERRLVHRDLAARNVLVKTPQHVKITDFGLAKLLGADEKEYHAEGGKVPIKWMALESILHRIYTHQSDVWSYGVTVWELMTFGSKPYEGIPASEISSVLEKGERLPQPPICTIDVYMIMVKCWMIDADSRPKFRELIAEFSKMARDPPRYLVIQGDDRMHLPSPTDSKFYRTLMEEEDMEDIVDADEYLVPHQGFFNSPSTSRTPLLSSLSATSNNSATTCIDRNGGHPVREDSFVQRYSSDPTGAFLEDSIDDSFLPAPEYVNQLVPKKASASAVQNPIYNNFSLTANSKVPTDSSYQNSHSTAVDNPEYLNTNQSPLAKTVFESSPYWIQAGNHQINLDNPDYQQDFLPKETKPNGLLKVPAAENPEYLRVAAPKSEYIEASA

Target ORF information:

RefSeq Version	XM_015618676.2
Organism	Parus major(Great Tit)
Definition	Parus major epidermal growth factor receptor (EGFR), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015618676.2

ORF Insert Sequence:

ATGGGCGGCC GCAGCCCCCT CGCTGCCGCC GGGCCCCGCG GCGCCGCTGT CCTGGTGCTG 
CTGCTGGGGC GCCTCGCTCT CTGCCTCGCC GTGGAGGGAA AGAAAGTTTG TCAAGGGACG 
AATAACAAGC TGACCCAGCT GGGACATGTG GAAGACCATT TCACCAGCCT GCAGAGGATG 
TACAATAATT GTGAGGTGGT ACTCAGCAAC CTGGAGATTA CATATGTGGA GCACAATCGT 
GACCTCTCTT TCCTGAAGAC AATACAGGAG GTTGCAGGCT ACGTGCTCAT CGCACTTAAC 
ATGGTGGATG TCATTCCCCT TGAGAACCTC CAGATCATCC GAGGCAACGT GCTGTATGAC 
AACTCTTACG CCCTGGCAGT TTTATCCAAC TACCACATGA ATAAAACCCA GGGGCTCCGG 
CAGCTGCCAA TGAAGCGGCT TTCAGAAATT CTCAATGGAG GTGTTAAAAT CAGCAACAAC 
CCCAAACTGT GCAACATGGA CACTGTTCTT TGGAATGACA TCATTGACAC AAGCAAGAAG 
CCTCCCACAG TGCTTGAATT TGCGAGCAAC CTATCTTCTT GTCCCAAATG CCATCAGAAC 
TGCACAGAAG ACCACTGCTG GGGCCCAGGC CAGCAAAACT GCCAGACATT AACAAAAGTC 
ATCTGTGCTC AGCAGTGCTC TGGTCGGTGC AGGGGAAAGG TGCCCAGCGA CTGCTGCCAC 
AACCAGTGTG CTGCGGGGTG CACCGGCCCT CGGGAGAGTG ACTGCCTGGC ATGCCGCAAG 
TTTCGGGATG ATGCTACGTG CAAGGATACA TGTCCTCCAT TGGTCCTGTA TAACCCCACC 
ACTTACCAGA TGGATGTGAA CCCTGAAGGA AAATACAGCT TTGGAGCCAC TTGTGTGAAG 
GAATGTCCAC ACAACTATGT GGTGACAGAC CATGGCTCCT GCGTTCGCTC GTGCAATGCT 
GATACTTACG AAGTTGAAGA AAATGGTGTT CGGAAATGTA AAAAGTGTGA TGGGCTATGC 
AGCAAAGTGT GCAATGGCAT CGGAATAGGT GAGCTTAAAG GGATCCTCTC CATAAATGCC 
ACAAATATCG ACTCCTTCAA AAACTGTACC AAGATCAATG GGGATGTCAG CATTCTCCCA 
GTTGCATTTC TAGGTGATGC CTTCACGAAG ACCCTACCTT TGGATCCCAA GAAGTTGGAT 
GTCTTCAAAA CAGTCAAAGA AATATCAGGA TTTTTGTTGA TTCAAGCCTG GCCTGAGAAT 
GCTACTGATC TCTATGCTTT TGAAAATCTG GAGATTATAC GAGGCAGAAC AAAGCAACAT 
GGCCAGTATT CCCTTGCCGT TGTTAACTTG AAAATAAAGT CCCTAGGGCT GCGGTCCCTC 
AAGGAAATAA GTGATGGAGA TGTTGCCATT ATGAGGAACA AGAACCTCTG CTATGCTGAC 
ACCATGGACT GGCACAGCCT GTTTGCAACT GAGAGCCAGA AAACGAAGAT TTTACAGAAT 
AGAGATAAAA ATGAGTGTGC TGCTGCCAAG CATGTTTGTG ACCCCCTGTG CTCAGATGTA 
GGCTGCTGGG GCCCAGGGCC CTTCCACTGC TTCTCCTGCA GGTTTTTTGA TCGTCAGAAG 
GAATGTGTGA AACAGTGCAA CATCCTGCAA GGGGAGCCAA GAGAGTTTGA AAAGGACTCC 
AAGTGCCTGC CCTGCCACCC TGAGTGCCTG GTGCAAAACT CCACCATGTA CAATGCGACC 
TGCACTGGAC CTGGCCCTGA CAATTGCATG AAGTGTGCCC ATTTTATAGA TGGCCCCCAC 
TGTGTGAAAT CCTGCCCAGC AGGGGTTCTG GGTGAGAATG ACACCCTCGT CTGGAAATAT 
GCCGATGGGA ATAATGTTTG CCAGCTCTGC CATCCAAACT GCACCCGGGG GTGCAAAGGG 
CCAGGTCTTG AAGGCTGTCC AAATGGCTCC AAGATCCCGT CTATCGCAGC CGGTGTTGTC 
GGAGGTCTCC TGTGCCTGGT GGTGGTGGGC CTGGGCATCG GCCTGTACCT GCGGAGACGC 
CACATCGTCA GAAAGCGGAC CCTGCGCCGG CTGCTGCAGG AGAGGGAGCT TGTTGAACCA 
CTGACACCCA GCGGGGAGGC ACCAAACCAG GCTCATCTGA GAATTTTAAA GGAAACAGAA 
TTTAAAAAGG TCAAAGTTTT AGGCTCTGGA GCTTTTGGCA CTGTTTATAA GGGACTTTGG 
ATCCCAGAAG GAGAGAAGGT TAAAATTCCT GTTGCTATTA AAGAGTTGAG AGAGGCTACA 
TCACCAAAAG CCAACAAGGA AATACTTGAT GAAGCTTATG TGATGGCTAG TGTTGACAAT 
CCTCATGTGT GTCGCTTGTT GGGCATCTGC CTCACTTCAA CTGTCCAGCT CATCACACAG 
CTGATGCCTT ACGGCTGCCT CCTGGATTAC ATCCGAGAGC ACAAGGACAA CATCGGCTCT 
CAGTACCTCC TCAACTGGTG TGTGCAGATT GCAAAGGGAA TGAACTATCT GGAGGAACGG 
CGCCTGGTGC ACCGGGACCT TGCTGCAAGG AATGTCCTCG TTAAGACTCC TCAGCATGTG 
AAAATCACGG ACTTTGGGCT GGCAAAGCTG CTCGGTGCAG ACGAGAAGGA GTATCATGCT 
GAGGGAGGCA AGGTTCCTAT TAAATGGATG GCATTGGAGT CAATTTTACA CCGGATTTAC 
ACTCATCAAA GTGATGTCTG GAGTTATGGT GTGACAGTCT GGGAGCTGAT GACATTTGGG 
TCCAAGCCAT ATGAAGGGAT CCCTGCCAGT GAGATCTCCT CTGTCCTGGA GAAGGGCGAG 
CGTTTGCCGC AGCCCCCCAT CTGTACCATC GACGTCTACA TGATCATGGT CAAATGCTGG 
ATGATCGATG CAGACAGCCG TCCCAAGTTT CGTGAGCTGA TAGCTGAATT CTCAAAAATG 
GCCCGTGACC CTCCACGCTA TCTTGTTATA CAGGGAGATG ATAGGATGCA CCTGCCCAGC 
CCTACGGACT CCAAGTTTTA CCGCACCCTG ATGGAGGAGG AGGACATGGA AGATATAGTG 
GATGCAGATG AGTACCTTGT GCCACACCAG GGCTTTTTCA ACAGCCCTTC AACATCTCGC 
ACTCCTCTCT TGAGTTCATT GAGTGCTACC AGCAACAACT CTGCTACAAC CTGCATTGAC 
AGGAACGGGG GGCACCCTGT GCGGGAAGAC AGCTTTGTTC AGAGGTATAG CTCAGACCCA 
ACAGGTGCTT TCTTGGAGGA CAGCATAGAT GACAGCTTCC TACCAGCCCC AGAGTATGTA 
AACCAATTGG TGCCCAAGAA AGCATCTGCC TCAGCAGTCC AGAATCCAAT CTACAACAAC 
TTCTCTCTCA CAGCAAACTC AAAGGTCCCC ACAGACTCCA GCTACCAGAA TTCACACAGC 
ACAGCTGTGG ACAACCCCGA GTATCTCAAC ACCAACCAGT CTCCCCTGGC CAAGACAGTC 
TTTGAGAGCT CTCCCTACTG GATCCAAGCG GGCAACCACC AAATAAATCT GGACAATCCT 
GACTACCAGC AGGACTTCTT ACCGAAGGAA ACCAAACCTA ACGGTCTCTT AAAAGTTCCT 
GCAGCAGAAA ACCCAGAGTA CTTGAGGGTA GCGGCACCAA AAAGTGAATA CATTGAGGCC 
TCAGCATGA