LOC107453699 cDNA ORF clone, Parasteatoda tepidariorum(Common house spider)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC107453699
Entrez Gene ID	107453699
Full Name	hephaestin-like protein
Gene Type	protein-coding
Organism	Parasteatoda tepidariorum(Common house spider)

mRNA and Protein(s)

mRNA	Protein	Name
XM_021148174.1	XP_021003833.1	hephaestin-like protein
XM_016070618.1	XP_015926104.1	hephaestin-like protein

Pathways from BioSystems
Gene Ontology

The following LOC107453699 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC107453699 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OPa137735	XM_021148174.1 Latest version!	Parasteatoda tepidariorum hephaestin-like protein (LOC107453699), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OPa137735	XM_016070618.1 Latest version!	Parasteatoda tepidariorum hephaestin-like protein (LOC107453699), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC107453699 ( XM_021148174.1 ) cDNA ORF clone, Parasteatoda tepidariorum(Common house spider) -> XP_021003833.1 Parasteatoda tepidariorum hephaestin-like protein (LOC107453699), mRNA.

Price & Availability↑

CloneID	OPa137735
Clone ID Related Accession (Same CDS sequence)	XM_021148174.1 , XM_016070618.1
Accession Version	XM_021148174.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3306bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-05-14
Organism	Parasteatoda tepidariorum(common house spider)
Product	hephaestin-like protein
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018381650.1) and transcript sequence (IAAA01042378.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Parasteatoda tepidariorum Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 1% of CDS bases assembly gap :: added 967 transcript bases to patch genome assembly gap ##RefSeq-Attributes-END##

ATGAGGTGCT TTTCGTGGTG CTATATCAGC GTGTTTTTGC TCGTATTATG GGCAGTGGAC 
GAGTGCTTAG GACAATTTTT CGGTGACCCG GGATGGGGGG CACCGCCAAT TAGAAAGAAA 
CATCGTGCGA AACCAGGATT CAAGCAAAAT TATGACTCTA ATATTGAAAA ATATCTACGG 
AAAGCTAAGA CTGTTAGACA TTATTATGTT GCTGCTATTG AAGTTGATTG GGATTATGCT 
CCTGGAGGAA ATTTACTCGG TGAAAGGGCA GAAGACTTGT CAAAGAGATA TTTTCAAGTC 
AGTCAAAATC CCTTACGTCT GGGCAAGGTG TACAAGAAAG CTATTTACAG AGAATTCACC 
GATGAGACTT ATACTATAAT GAAAGCCCGT GAGCCATGGC AAGGTATCCT CGGACCAACT 
ATCAGAGGAG AAGTAGGTGA TATGGCCATC GTCCACTTCT GGAACAATGC CTCCATGCTT 
CTTTCTATGC ATCCTCACGG TTCTCGATAC ATCAAAGTTC ACGAAGGAGC TCTTTATGAG 
GATGGTACTG GACCACAGGA TAAAGTGGAT GATATGGTAC CACCAAATGG ACGACATTCT 
TACCACTGGT TCTTTATTCC TGAAGATGGT CCCAGTCCTA CTGATCCAGA TTGTCTCACG 
TGGATGTACC ATTCTCACAG GAATGCTGAT ACTGATATTC ATGCTGGTCT TCTTGGGCCA 
CTCATCGTCT GTAGACCAGG CACTTTAGAT ATGTATGACA GACCTAGAAA TGTGGACCGA 
GATTTCATTC TCGTTTTTAT GATTTTCGAT GAAAATGATA GTTTATACAT TGATGAAAAC 
ATTGACAGAA TGGCACCACA CTTAACAGAA GAGGAGAGAC GAAGAATTAG AGCTGACATT 
AGTTTTAGAA GATCTAATAT GATGTTTTCT GTAAATGGTA TGAGCTTCGG CAATCTTAGA 
GGACTGGATA TGTGTTTGGG AGACAAAGTA GGTTGGCACG TCGTCGGAAT GGGTAATGAA 
GTTGATGTTC ACAGTATCAG TTTCGAAGGC CAAGTCTTCA CAATAAATGG AATGACAACT 
GAAATCATTA GCGTTTTTCC TGCTAAGTTT GTAACAAGTC TGATGATAGC TAGCAGAGAA 
GGTACCTTTC CATTTAGGTG TGAAGTCGGT TTGCATGGAT TAGCTGGTAT GGAATCAGTA 
TTTTCTATCA GCCGTTGTCC ACATTCCAGA CCAGTTGTTA CAAATCTGAA TGGTGTAACA 
AGAAATTATT ACATAGCTGC GGAGGAAGTT GAATGGAACT ATGGGCCATC AGGAATAAAC 
AAGTTTAATG GTGTACCATT AGTAGAAGAT AGAGCTGCTT CCATTTTCTT TGTACGAAAT 
GAAACAAGAA TAGGAGGAAC ATACAGAAAG GTTGTTTATA GAGAATATAG TGATGCATCT 
TTCACGCTGA AAAAAGTTCG GGGTGAAGAT GAAACTCACC TTGCAATGTT AGGACCTACC 
CTCAGGGGAG AAGTTGGTGA TGTCATCCGA GTCACATTCC TCAACGCAGC CACTAAGCTG 
TATAATATGC ATACCCATGG AGCTTTTTAC GAACCAGAAA CGCTGCCTGT CGAACCTGGC 
ACCACTAAAA TTTACACATG GACAGTAGCA GAGCAATACG GACCAACCCA TACAGATCCA 
GAATGTTTAA CGTGGCGATA CTATTCAAGT GTGGATGTTG AACGTGATAT GTGGGATGGT 
TTAAGTGGTA AACTTTTAAC ATGCAGGAAA GGAACACTTG ATAGGAGTGG AAAACAGAAA 
TATGTGGATA AAGAATTTGC TCTGCTGTTT TCTGTCATGG ATGAAAGTTT ATCACATTAT 
TTGCCAGAAA ATGTAATGCA ATATGCATAT AGACCAGACC TTGTCAATAC ATCAGATCCT 
ATGTTTATCG AATCAAACCA AATGCACTCT ATCAACGGCA GAGTTTGGGG AAATCTTGAA 
GGTCTTAGAA TGTGCGTTGG AGACAAAGTA AGCTGGCATC TTGCTACATA TGGAAAAGCT 
ATAGATATAC ACACACCATA CTTCCACGGC AACACTTTCC AAATAAGTGG TAAATTCAGA 
GATGCTATCA GTGTTGCACC AAGCACACAT TACACAGTTG TTATGCATGC TGATAATCCT 
GGTACATGGG CTTTAGACTG CCGAATAAAC AATCATTTTG CTGGAGGTAT GAGAGCTATT 
TACCATGTAG AAGATTGTGG TCACGGATTA TATCCACCAA GGCAGATCTT TTATGATCAT 
AAGAAAGTTA GAGAATATTT CATTGCAGCA ATAGAAATCG AATGGGACTA TGCACCCAAC 
CCTAGGCACA TAATTGATGG TTCTAATCTA TTAGAAAACG AAGAAAGTCG ACCATTTGTA 
GAGAATGGGC CACAACGTAT TGGCAGAATA TACAAAAAAG CAGTCTTCCG AGAATTCACT 
GATTCCTCTT TTACTCACAT GAAACCGAGA GGAAGAGATG AACAGCATTT GGAACTCATT 
GGTCCATACA TCAAAGCTGA AGTTGGCGAT CTCATTCGAG TTGTGTTCAA GAACTTGGCA 
TCAAGACCCT ACAATATCCA TCCCCACGGA GTCTTGTTAG AAAAGTACGA GTCCTACGCA 
GATGCTCTGG GTGAAGACGA AGAACCAGTG GCTCCTGGTG ACACACATGT TTATGAGTGG 
CCAGTACCAG AACGAGCCGG ACCAGGAAGA AATGGATTCA ACTGCTCTTC ATGGGCTTAC 
TATTCAGCTG TGGATGTTGT GAAGGATACC AACTCTGGTC TTGTTGGTCC TTTAATAACA 
TGCAAGCCTG GTACTTTGAC ATCCAAAGGA CGACGAAGAG ATTTAGATCA TGAATTTGCT 
GTTCTATTTA CCATCTTCGA TGAGAATAGA AGTTGGTACA TCCAAGAAAA TACTTATAAG 
TATGCCACCA GGCCGGAAAC AGTAAATCCT TTGGATCCTG GTTTCATTTT AAGTAACAGA 
ATGCACGCTG TGAATGGTAG ACTGTATGGA TCTTTGGACG GTCTTGTTGC AAGGCGGAAC 
ACATTGGCTG CATGGTATCT CATAGGTATG GGAACAGAAG TAGATATGCA CACTGCTCAC 
TTCCACGGTC AGACCTTCCT ACGTCGAACA GACAGTACTC GACGTTCTGA CGTCGTTTAC 
CTTTTCCCTG GTGTGTTTGA AACAGTAGAA ATGATTATGG ATAATCCTGG TACATGGATC 
CTGCATTGTC ACGTAGACGA CCATATGAGA GCGGGCATGG CGGTCACCTA CACCGTGGAG 
CGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021003833.1
CDS	233..3538
Translation	MRCFSWCYISVFLLVLWAVDECLGQFFGDPGWGAPPIRKKHRAKPGFKQNYDSNIEKYLRKAKTVRHYYVAAIEVDWDYAPGGNLLGERAEDLSKRYFQVSQNPLRLGKVYKKAIYREFTDETYTIMKAREPWQGILGPTIRGEVGDMAIVHFWNNASMLLSMHPHGSRYIKVHEGALYEDGTGPQDKVDDMVPPNGRHSYHWFFIPEDGPSPTDPDCLTWMYHSHRNADTDIHAGLLGPLIVCRPGTLDMYDRPRNVDRDFILVFMIFDENDSLYIDENIDRMAPHLTEEERRRIRADISFRRSNMMFSVNGMSFGNLRGLDMCLGDKVGWHVVGMGNEVDVHSISFEGQVFTINGMTTEIISVFPAKFVTSLMIASREGTFPFRCEVGLHGLAGMESVFSISRCPHSRPVVTNLNGVTRNYYIAAEEVEWNYGPSGINKFNGVPLVEDRAASIFFVRNETRIGGTYRKVVYREYSDASFTLKKVRGEDETHLAMLGPTLRGEVGDVIRVTFLNAATKLYNMHTHGAFYEPETLPVEPGTTKIYTWTVAEQYGPTHTDPECLTWRYYSSVDVERDMWDGLSGKLLTCRKGTLDRSGKQKYVDKEFALLFSVMDESLSHYLPENVMQYAYRPDLVNTSDPMFIESNQMHSINGRVWGNLEGLRMCVGDKVSWHLATYGKAIDIHTPYFHGNTFQISGKFRDAISVAPSTHYTVVMHADNPGTWALDCRINNHFAGGMRAIYHVEDCGHGLYPPRQIFYDHKKVREYFIAAIEIEWDYAPNPRHIIDGSNLLENEESRPFVENGPQRIGRIYKKAVFREFTDSSFTHMKPRGRDEQHLELIGPYIKAEVGDLIRVVFKNLASRPYNIHPHGVLLEKYESYADALGEDEEPVAPGDTHVYEWPVPERAGPGRNGFNCSSWAYYSAVDVVKDTNSGLVGPLITCKPGTLTSKGRRRDLDHEFAVLFTIFDENRSWYIQENTYKYATRPETVNPLDPGFILSNRMHAVNGRLYGSLDGLVARRNTLAAWYLIGMGTEVDMHTAHFHGQTFLRRTDSTRRSDVVYLFPGVFETVEMIMDNPGTWILHCHVDDHMRAGMAVTYTVER

Target ORF information:

RefSeq Version	XM_021148174.1
Organism	Parasteatoda tepidariorum(common house spider)
Definition	Parasteatoda tepidariorum hephaestin-like protein (LOC107453699), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021148174.1

ORF Insert Sequence:

ATGAGGTGCT TTTCGTGGTG CTATATCAGC GTGTTTTTGC TCGTATTATG GGCAGTGGAC 
GAGTGCTTAG GACAATTTTT CGGTGACCCG GGATGGGGGG CACCGCCAAT TAGAAAGAAA 
CATCGTGCGA AACCAGGATT CAAGCAAAAT TATGACTCTA ATATTGAAAA ATATCTACGG 
AAAGCTAAGA CTGTTAGACA TTATTATGTT GCTGCTATTG AAGTTGATTG GGATTATGCT 
CCTGGAGGAA ATTTACTCGG TGAAAGGGCA GAAGACTTGT CAAAGAGATA TTTTCAAGTC 
AGTCAAAATC CCTTACGTCT GGGCAAGGTG TACAAGAAAG CTATTTACAG AGAATTCACC 
GATGAGACTT ATACTATAAT GAAAGCCCGT GAGCCATGGC AAGGTATCCT CGGACCAACT 
ATCAGAGGAG AAGTAGGTGA TATGGCCATC GTCCACTTCT GGAACAATGC CTCCATGCTT 
CTTTCTATGC ATCCTCACGG TTCTCGATAC ATCAAAGTTC ACGAAGGAGC TCTTTATGAG 
GATGGTACTG GACCACAGGA TAAAGTGGAT GATATGGTAC CACCAAATGG ACGACATTCT 
TACCACTGGT TCTTTATTCC TGAAGATGGT CCCAGTCCTA CTGATCCAGA TTGTCTCACG 
TGGATGTACC ATTCTCACAG GAATGCTGAT ACTGATATTC ATGCTGGTCT TCTTGGGCCA 
CTCATCGTCT GTAGACCAGG CACTTTAGAT ATGTATGACA GACCTAGAAA TGTGGACCGA 
GATTTCATTC TCGTTTTTAT GATTTTCGAT GAAAATGATA GTTTATACAT TGATGAAAAC 
ATTGACAGAA TGGCACCACA CTTAACAGAA GAGGAGAGAC GAAGAATTAG AGCTGACATT 
AGTTTTAGAA GATCTAATAT GATGTTTTCT GTAAATGGTA TGAGCTTCGG CAATCTTAGA 
GGACTGGATA TGTGTTTGGG AGACAAAGTA GGTTGGCACG TCGTCGGAAT GGGTAATGAA 
GTTGATGTTC ACAGTATCAG TTTCGAAGGC CAAGTCTTCA CAATAAATGG AATGACAACT 
GAAATCATTA GCGTTTTTCC TGCTAAGTTT GTAACAAGTC TGATGATAGC TAGCAGAGAA 
GGTACCTTTC CATTTAGGTG TGAAGTCGGT TTGCATGGAT TAGCTGGTAT GGAATCAGTA 
TTTTCTATCA GCCGTTGTCC ACATTCCAGA CCAGTTGTTA CAAATCTGAA TGGTGTAACA 
AGAAATTATT ACATAGCTGC GGAGGAAGTT GAATGGAACT ATGGGCCATC AGGAATAAAC 
AAGTTTAATG GTGTACCATT AGTAGAAGAT AGAGCTGCTT CCATTTTCTT TGTACGAAAT 
GAAACAAGAA TAGGAGGAAC ATACAGAAAG GTTGTTTATA GAGAATATAG TGATGCATCT 
TTCACGCTGA AAAAAGTTCG GGGTGAAGAT GAAACTCACC TTGCAATGTT AGGACCTACC 
CTCAGGGGAG AAGTTGGTGA TGTCATCCGA GTCACATTCC TCAACGCAGC CACTAAGCTG 
TATAATATGC ATACCCATGG AGCTTTTTAC GAACCAGAAA CGCTGCCTGT CGAACCTGGC 
ACCACTAAAA TTTACACATG GACAGTAGCA GAGCAATACG GACCAACCCA TACAGATCCA 
GAATGTTTAA CGTGGCGATA CTATTCAAGT GTGGATGTTG AACGTGATAT GTGGGATGGT 
TTAAGTGGTA AACTTTTAAC ATGCAGGAAA GGAACACTTG ATAGGAGTGG AAAACAGAAA 
TATGTGGATA AAGAATTTGC TCTGCTGTTT TCTGTCATGG ATGAAAGTTT ATCACATTAT 
TTGCCAGAAA ATGTAATGCA ATATGCATAT AGACCAGACC TTGTCAATAC ATCAGATCCT 
ATGTTTATCG AATCAAACCA AATGCACTCT ATCAACGGCA GAGTTTGGGG AAATCTTGAA 
GGTCTTAGAA TGTGCGTTGG AGACAAAGTA AGCTGGCATC TTGCTACATA TGGAAAAGCT 
ATAGATATAC ACACACCATA CTTCCACGGC AACACTTTCC AAATAAGTGG TAAATTCAGA 
GATGCTATCA GTGTTGCACC AAGCACACAT TACACAGTTG TTATGCATGC TGATAATCCT 
GGTACATGGG CTTTAGACTG CCGAATAAAC AATCATTTTG CTGGAGGTAT GAGAGCTATT 
TACCATGTAG AAGATTGTGG TCACGGATTA TATCCACCAA GGCAGATCTT TTATGATCAT 
AAGAAAGTTA GAGAATATTT CATTGCAGCA ATAGAAATCG AATGGGACTA TGCACCCAAC 
CCTAGGCACA TAATTGATGG TTCTAATCTA TTAGAAAACG AAGAAAGTCG ACCATTTGTA 
GAGAATGGGC CACAACGTAT TGGCAGAATA TACAAAAAAG CAGTCTTCCG AGAATTCACT 
GATTCCTCTT TTACTCACAT GAAACCGAGA GGAAGAGATG AACAGCATTT GGAACTCATT 
GGTCCATACA TCAAAGCTGA AGTTGGCGAT CTCATTCGAG TTGTGTTCAA GAACTTGGCA 
TCAAGACCCT ACAATATCCA TCCCCACGGA GTCTTGTTAG AAAAGTACGA GTCCTACGCA 
GATGCTCTGG GTGAAGACGA AGAACCAGTG GCTCCTGGTG ACACACATGT TTATGAGTGG 
CCAGTACCAG AACGAGCCGG ACCAGGAAGA AATGGATTCA ACTGCTCTTC ATGGGCTTAC 
TATTCAGCTG TGGATGTTGT GAAGGATACC AACTCTGGTC TTGTTGGTCC TTTAATAACA 
TGCAAGCCTG GTACTTTGAC ATCCAAAGGA CGACGAAGAG ATTTAGATCA TGAATTTGCT 
GTTCTATTTA CCATCTTCGA TGAGAATAGA AGTTGGTACA TCCAAGAAAA TACTTATAAG 
TATGCCACCA GGCCGGAAAC AGTAAATCCT TTGGATCCTG GTTTCATTTT AAGTAACAGA 
ATGCACGCTG TGAATGGTAG ACTGTATGGA TCTTTGGACG GTCTTGTTGC AAGGCGGAAC 
ACATTGGCTG CATGGTATCT CATAGGTATG GGAACAGAAG TAGATATGCA CACTGCTCAC 
TTCCACGGTC AGACCTTCCT ACGTCGAACA GACAGTACTC GACGTTCTGA CGTCGTTTAC 
CTTTTCCCTG GTGTGTTTGA AACAGTAGAA ATGATTATGG ATAATCCTGG TACATGGATC 
CTGCATTGTC ACGTAGACGA CCATATGAGA GCGGGCATGG CGGTCACCTA CACCGTGGAG 
CGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC107453699 ( XM_016070618.1 ) cDNA ORF clone, Parasteatoda tepidariorum(Common house spider) -> XP_015926104.1 Parasteatoda tepidariorum hephaestin-like protein (LOC107453699), mRNA.

Price & Availability↑

CloneID	OPa137735
Clone ID Related Accession (Same CDS sequence)	XM_021148174.1 , XM_016070618.1
Accession Version	XM_016070618.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3306bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-03-24
Organism	Parasteatoda tepidariorum(Common house spider)
Product	hephaestin-like protein
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015486065.1) and transcript sequence (IAAA01042378.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Parasteatoda tepidariorum Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 1% of CDS bases assembly gap :: added 967 transcript bases to patch genome assembly gap ##RefSeq-Attributes-END##

ATGAGGTGCT TTTCGTGGTG CTATATCAGC GTGTTTTTGC TCGTATTATG GGCAGTGGAC 
GAGTGCTTAG GACAATTTTT CGGTGACCCG GGATGGGGGG CACCGCCAAT TAGAAAGAAA 
CATCGTGCGA AACCAGGATT CAAGCAAAAT TATGACTCTA ATATTGAAAA ATATCTACGG 
AAAGCTAAGA CTGTTAGACA TTATTATGTT GCTGCTATTG AAGTTGATTG GGATTATGCT 
CCTGGAGGAA ATTTACTCGG TGAAAGGGCA GAAGACTTGT CAAAGAGATA TTTTCAAGTC 
AGTCAAAATC CCTTACGTCT GGGCAAGGTG TACAAGAAAG CTATTTACAG AGAATTCACC 
GATGAGACTT ATACTATAAT GAAAGCCCGT GAGCCATGGC AAGGTATCCT CGGACCAACT 
ATCAGAGGAG AAGTAGGTGA TATGGCCATC GTCCACTTCT GGAACAATGC CTCCATGCTT 
CTTTCTATGC ATCCTCACGG TTCTCGATAC ATCAAAGTTC ACGAAGGAGC TCTTTATGAG 
GATGGTACTG GACCACAGGA TAAAGTGGAT GATATGGTAC CACCAAATGG ACGACATTCT 
TACCACTGGT TCTTTATTCC TGAAGATGGT CCCAGTCCTA CTGATCCAGA TTGTCTCACG 
TGGATGTACC ATTCTCACAG GAATGCTGAT ACTGATATTC ATGCTGGTCT TCTTGGGCCA 
CTCATCGTCT GTAGACCAGG CACTTTAGAT ATGTATGACA GACCTAGAAA TGTGGACCGA 
GATTTCATTC TCGTTTTTAT GATTTTCGAT GAAAATGATA GTTTATACAT TGATGAAAAC 
ATTGACAGAA TGGCACCACA CTTAACAGAA GAGGAGAGAC GAAGAATTAG AGCTGACATT 
AGTTTTAGAA GATCTAATAT GATGTTTTCT GTAAATGGTA TGAGCTTCGG CAATCTTAGA 
GGACTGGATA TGTGTTTGGG AGACAAAGTA GGTTGGCACG TCGTCGGAAT GGGTAATGAA 
GTTGATGTTC ACAGTATCAG TTTCGAAGGC CAAGTCTTCA CAATAAATGG AATGACAACT 
GAAATCATTA GCGTTTTTCC TGCTAAGTTT GTAACAAGTC TGATGATAGC TAGCAGAGAA 
GGTACCTTTC CATTTAGGTG TGAAGTCGGT TTGCATGGAT TAGCTGGTAT GGAATCAGTA 
TTTTCTATCA GCCGTTGTCC ACATTCCAGA CCAGTTGTTA CAAATCTGAA TGGTGTAACA 
AGAAATTATT ACATAGCTGC GGAGGAAGTT GAATGGAACT ATGGGCCATC AGGAATAAAC 
AAGTTTAATG GTGTACCATT AGTAGAAGAT AGAGCTGCTT CCATTTTCTT TGTACGAAAT 
GAAACAAGAA TAGGAGGAAC ATACAGAAAG GTTGTTTATA GAGAATATAG TGATGCATCT 
TTCACGCTGA AAAAAGTTCG GGGTGAAGAT GAAACTCACC TTGCAATGTT AGGACCTACC 
CTCAGGGGAG AAGTTGGTGA TGTCATCCGA GTCACATTCC TCAACGCAGC CACTAAGCTG 
TATAATATGC ATACCCATGG AGCTTTTTAC GAACCAGAAA CGCTGCCTGT CGAACCTGGC 
ACCACTAAAA TTTACACATG GACAGTAGCA GAGCAATACG GACCAACCCA TACAGATCCA 
GAATGTTTAA CGTGGCGATA CTATTCAAGT GTGGATGTTG AACGTGATAT GTGGGATGGT 
TTAAGTGGTA AACTTTTAAC ATGCAGGAAA GGAACACTTG ATAGGAGTGG AAAACAGAAA 
TATGTGGATA AAGAATTTGC TCTGCTGTTT TCTGTCATGG ATGAAAGTTT ATCACATTAT 
TTGCCAGAAA ATGTAATGCA ATATGCATAT AGACCAGACC TTGTCAATAC ATCAGATCCT 
ATGTTTATCG AATCAAACCA AATGCACTCT ATCAACGGCA GAGTTTGGGG AAATCTTGAA 
GGTCTTAGAA TGTGCGTTGG AGACAAAGTA AGCTGGCATC TTGCTACATA TGGAAAAGCT 
ATAGATATAC ACACACCATA CTTCCACGGC AACACTTTCC AAATAAGTGG TAAATTCAGA 
GATGCTATCA GTGTTGCACC AAGCACACAT TACACAGTTG TTATGCATGC TGATAATCCT 
GGTACATGGG CTTTAGACTG CCGAATAAAC AATCATTTTG CTGGAGGTAT GAGAGCTATT 
TACCATGTAG AAGATTGTGG TCACGGATTA TATCCACCAA GGCAGATCTT TTATGATCAT 
AAGAAAGTTA GAGAATATTT CATTGCAGCA ATAGAAATCG AATGGGACTA TGCACCCAAC 
CCTAGGCACA TAATTGATGG TTCTAATCTA TTAGAAAACG AAGAAAGTCG ACCATTTGTA 
GAGAATGGGC CACAACGTAT TGGCAGAATA TACAAAAAAG CAGTCTTCCG AGAATTCACT 
GATTCCTCTT TTACTCACAT GAAACCGAGA GGAAGAGATG AACAGCATTT GGAACTCATT 
GGTCCATACA TCAAAGCTGA AGTTGGCGAT CTCATTCGAG TTGTGTTCAA GAACTTGGCA 
TCAAGACCCT ACAATATCCA TCCCCACGGA GTCTTGTTAG AAAAGTACGA GTCCTACGCA 
GATGCTCTGG GTGAAGACGA AGAACCAGTG GCTCCTGGTG ACACACATGT TTATGAGTGG 
CCAGTACCAG AACGAGCCGG ACCAGGAAGA AATGGATTCA ACTGCTCTTC ATGGGCTTAC 
TATTCAGCTG TGGATGTTGT GAAGGATACC AACTCTGGTC TTGTTGGTCC TTTAATAACA 
TGCAAGCCTG GTACTTTGAC ATCCAAAGGA CGACGAAGAG ATTTAGATCA TGAATTTGCT 
GTTCTATTTA CCATCTTCGA TGAGAATAGA AGTTGGTACA TCCAAGAAAA TACTTATAAG 
TATGCCACCA GGCCGGAAAC AGTAAATCCT TTGGATCCTG GTTTCATTTT AAGTAACAGA 
ATGCACGCTG TGAATGGTAG ACTGTATGGA TCTTTGGACG GTCTTGTTGC AAGGCGGAAC 
ACATTGGCTG CATGGTATCT CATAGGTATG GGAACAGAAG TAGATATGCA CACTGCTCAC 
TTCCACGGTC AGACCTTCCT ACGTCGAACA GACAGTACTC GACGTTCTGA CGTCGTTTAC 
CTTTTCCCTG GTGTGTTTGA AACAGTAGAA ATGATTATGG ATAATCCTGG TACATGGATC 
CTGCATTGTC ACGTAGACGA CCATATGAGA GCGGGCATGG CGGTCACCTA CACCGTGGAG 
CGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015926104.1
CDS	233..3538
Translation	MRCFSWCYISVFLLVLWAVDECLGQFFGDPGWGAPPIRKKHRAKPGFKQNYDSNIEKYLRKAKTVRHYYVAAIEVDWDYAPGGNLLGERAEDLSKRYFQVSQNPLRLGKVYKKAIYREFTDETYTIMKAREPWQGILGPTIRGEVGDMAIVHFWNNASMLLSMHPHGSRYIKVHEGALYEDGTGPQDKVDDMVPPNGRHSYHWFFIPEDGPSPTDPDCLTWMYHSHRNADTDIHAGLLGPLIVCRPGTLDMYDRPRNVDRDFILVFMIFDENDSLYIDENIDRMAPHLTEEERRRIRADISFRRSNMMFSVNGMSFGNLRGLDMCLGDKVGWHVVGMGNEVDVHSISFEGQVFTINGMTTEIISVFPAKFVTSLMIASREGTFPFRCEVGLHGLAGMESVFSISRCPHSRPVVTNLNGVTRNYYIAAEEVEWNYGPSGINKFNGVPLVEDRAASIFFVRNETRIGGTYRKVVYREYSDASFTLKKVRGEDETHLAMLGPTLRGEVGDVIRVTFLNAATKLYNMHTHGAFYEPETLPVEPGTTKIYTWTVAEQYGPTHTDPECLTWRYYSSVDVERDMWDGLSGKLLTCRKGTLDRSGKQKYVDKEFALLFSVMDESLSHYLPENVMQYAYRPDLVNTSDPMFIESNQMHSINGRVWGNLEGLRMCVGDKVSWHLATYGKAIDIHTPYFHGNTFQISGKFRDAISVAPSTHYTVVMHADNPGTWALDCRINNHFAGGMRAIYHVEDCGHGLYPPRQIFYDHKKVREYFIAAIEIEWDYAPNPRHIIDGSNLLENEESRPFVENGPQRIGRIYKKAVFREFTDSSFTHMKPRGRDEQHLELIGPYIKAEVGDLIRVVFKNLASRPYNIHPHGVLLEKYESYADALGEDEEPVAPGDTHVYEWPVPERAGPGRNGFNCSSWAYYSAVDVVKDTNSGLVGPLITCKPGTLTSKGRRRDLDHEFAVLFTIFDENRSWYIQENTYKYATRPETVNPLDPGFILSNRMHAVNGRLYGSLDGLVARRNTLAAWYLIGMGTEVDMHTAHFHGQTFLRRTDSTRRSDVVYLFPGVFETVEMIMDNPGTWILHCHVDDHMRAGMAVTYTVER

Target ORF information:

RefSeq Version	XM_016070618.1
Organism	Parasteatoda tepidariorum(Common house spider)
Definition	Parasteatoda tepidariorum hephaestin-like protein (LOC107453699), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016070618.1

ORF Insert Sequence:

ATGAGGTGCT TTTCGTGGTG CTATATCAGC GTGTTTTTGC TCGTATTATG GGCAGTGGAC 
GAGTGCTTAG GACAATTTTT CGGTGACCCG GGATGGGGGG CACCGCCAAT TAGAAAGAAA 
CATCGTGCGA AACCAGGATT CAAGCAAAAT TATGACTCTA ATATTGAAAA ATATCTACGG 
AAAGCTAAGA CTGTTAGACA TTATTATGTT GCTGCTATTG AAGTTGATTG GGATTATGCT 
CCTGGAGGAA ATTTACTCGG TGAAAGGGCA GAAGACTTGT CAAAGAGATA TTTTCAAGTC 
AGTCAAAATC CCTTACGTCT GGGCAAGGTG TACAAGAAAG CTATTTACAG AGAATTCACC 
GATGAGACTT ATACTATAAT GAAAGCCCGT GAGCCATGGC AAGGTATCCT CGGACCAACT 
ATCAGAGGAG AAGTAGGTGA TATGGCCATC GTCCACTTCT GGAACAATGC CTCCATGCTT 
CTTTCTATGC ATCCTCACGG TTCTCGATAC ATCAAAGTTC ACGAAGGAGC TCTTTATGAG 
GATGGTACTG GACCACAGGA TAAAGTGGAT GATATGGTAC CACCAAATGG ACGACATTCT 
TACCACTGGT TCTTTATTCC TGAAGATGGT CCCAGTCCTA CTGATCCAGA TTGTCTCACG 
TGGATGTACC ATTCTCACAG GAATGCTGAT ACTGATATTC ATGCTGGTCT TCTTGGGCCA 
CTCATCGTCT GTAGACCAGG CACTTTAGAT ATGTATGACA GACCTAGAAA TGTGGACCGA 
GATTTCATTC TCGTTTTTAT GATTTTCGAT GAAAATGATA GTTTATACAT TGATGAAAAC 
ATTGACAGAA TGGCACCACA CTTAACAGAA GAGGAGAGAC GAAGAATTAG AGCTGACATT 
AGTTTTAGAA GATCTAATAT GATGTTTTCT GTAAATGGTA TGAGCTTCGG CAATCTTAGA 
GGACTGGATA TGTGTTTGGG AGACAAAGTA GGTTGGCACG TCGTCGGAAT GGGTAATGAA 
GTTGATGTTC ACAGTATCAG TTTCGAAGGC CAAGTCTTCA CAATAAATGG AATGACAACT 
GAAATCATTA GCGTTTTTCC TGCTAAGTTT GTAACAAGTC TGATGATAGC TAGCAGAGAA 
GGTACCTTTC CATTTAGGTG TGAAGTCGGT TTGCATGGAT TAGCTGGTAT GGAATCAGTA 
TTTTCTATCA GCCGTTGTCC ACATTCCAGA CCAGTTGTTA CAAATCTGAA TGGTGTAACA 
AGAAATTATT ACATAGCTGC GGAGGAAGTT GAATGGAACT ATGGGCCATC AGGAATAAAC 
AAGTTTAATG GTGTACCATT AGTAGAAGAT AGAGCTGCTT CCATTTTCTT TGTACGAAAT 
GAAACAAGAA TAGGAGGAAC ATACAGAAAG GTTGTTTATA GAGAATATAG TGATGCATCT 
TTCACGCTGA AAAAAGTTCG GGGTGAAGAT GAAACTCACC TTGCAATGTT AGGACCTACC 
CTCAGGGGAG AAGTTGGTGA TGTCATCCGA GTCACATTCC TCAACGCAGC CACTAAGCTG 
TATAATATGC ATACCCATGG AGCTTTTTAC GAACCAGAAA CGCTGCCTGT CGAACCTGGC 
ACCACTAAAA TTTACACATG GACAGTAGCA GAGCAATACG GACCAACCCA TACAGATCCA 
GAATGTTTAA CGTGGCGATA CTATTCAAGT GTGGATGTTG AACGTGATAT GTGGGATGGT 
TTAAGTGGTA AACTTTTAAC ATGCAGGAAA GGAACACTTG ATAGGAGTGG AAAACAGAAA 
TATGTGGATA AAGAATTTGC TCTGCTGTTT TCTGTCATGG ATGAAAGTTT ATCACATTAT 
TTGCCAGAAA ATGTAATGCA ATATGCATAT AGACCAGACC TTGTCAATAC ATCAGATCCT 
ATGTTTATCG AATCAAACCA AATGCACTCT ATCAACGGCA GAGTTTGGGG AAATCTTGAA 
GGTCTTAGAA TGTGCGTTGG AGACAAAGTA AGCTGGCATC TTGCTACATA TGGAAAAGCT 
ATAGATATAC ACACACCATA CTTCCACGGC AACACTTTCC AAATAAGTGG TAAATTCAGA 
GATGCTATCA GTGTTGCACC AAGCACACAT TACACAGTTG TTATGCATGC TGATAATCCT 
GGTACATGGG CTTTAGACTG CCGAATAAAC AATCATTTTG CTGGAGGTAT GAGAGCTATT 
TACCATGTAG AAGATTGTGG TCACGGATTA TATCCACCAA GGCAGATCTT TTATGATCAT 
AAGAAAGTTA GAGAATATTT CATTGCAGCA ATAGAAATCG AATGGGACTA TGCACCCAAC 
CCTAGGCACA TAATTGATGG TTCTAATCTA TTAGAAAACG AAGAAAGTCG ACCATTTGTA 
GAGAATGGGC CACAACGTAT TGGCAGAATA TACAAAAAAG CAGTCTTCCG AGAATTCACT 
GATTCCTCTT TTACTCACAT GAAACCGAGA GGAAGAGATG AACAGCATTT GGAACTCATT 
GGTCCATACA TCAAAGCTGA AGTTGGCGAT CTCATTCGAG TTGTGTTCAA GAACTTGGCA 
TCAAGACCCT ACAATATCCA TCCCCACGGA GTCTTGTTAG AAAAGTACGA GTCCTACGCA 
GATGCTCTGG GTGAAGACGA AGAACCAGTG GCTCCTGGTG ACACACATGT TTATGAGTGG 
CCAGTACCAG AACGAGCCGG ACCAGGAAGA AATGGATTCA ACTGCTCTTC ATGGGCTTAC 
TATTCAGCTG TGGATGTTGT GAAGGATACC AACTCTGGTC TTGTTGGTCC TTTAATAACA 
TGCAAGCCTG GTACTTTGAC ATCCAAAGGA CGACGAAGAG ATTTAGATCA TGAATTTGCT 
GTTCTATTTA CCATCTTCGA TGAGAATAGA AGTTGGTACA TCCAAGAAAA TACTTATAAG 
TATGCCACCA GGCCGGAAAC AGTAAATCCT TTGGATCCTG GTTTCATTTT AAGTAACAGA 
ATGCACGCTG TGAATGGTAG ACTGTATGGA TCTTTGGACG GTCTTGTTGC AAGGCGGAAC 
ACATTGGCTG CATGGTATCT CATAGGTATG GGAACAGAAG TAGATATGCA CACTGCTCAC 
TTCCACGGTC AGACCTTCCT ACGTCGAACA GACAGTACTC GACGTTCTGA CGTCGTTTAC 
CTTTTCCCTG GTGTGTTTGA AACAGTAGAA ATGATTATGG ATAATCCTGG TACATGGATC 
CTGCATTGTC ACGTAGACGA CCATATGAGA GCGGGCATGG CGGTCACCTA CACCGTGGAG 
CGCTGA