LOC109642528 cDNA ORF clone, Paralichthys olivaceus(Japanese flounder)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC109642528
Entrez Gene ID	109642528
Full Name	anion exchange protein 2-like
Gene Type	protein-coding
Organism	Paralichthys olivaceus(Japanese flounder)

mRNA and Protein(s)

mRNA	Protein	Name
XM_020107353.1	XP_019962912.1	anion exchange protein 2-like isoform X1
XM_020107354.1	XP_019962913.1	anion exchange protein 2-like isoform X2

Pathways from BioSystems
Gene Ontology

The following LOC109642528 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC109642528 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OPa256191	XM_020107353.1 Latest version!	Paralichthys olivaceus anion exchange protein 2-like (LOC109642528), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
OPa256192	XM_020107354.1 Latest version!	Paralichthys olivaceus anion exchange protein 2-like (LOC109642528), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC109642528 ( XM_020107353.1 ) cDNA ORF clone, Paralichthys olivaceus(Japanese flounder) -> XP_019962912.1 Paralichthys olivaceus anion exchange protein 2-like (LOC109642528), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OPa256191
Clone ID Related Accession (Same CDS sequence)	XM_020107353.1
Accession Version	XM_020107353.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2994bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-02-07
Organism	Paralichthys olivaceus(Japanese flounder)
Product	anion exchange protein 2-like isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_017860137.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Paralichthys olivaceus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCAACC CCAGCGTTCC CAACCAGATT ACAGATGCCA TGGCAGCGGT CATTCACAGT 
CCAGAGGCTC CTGCAGCCTC ATCACTACAT GGGACTCCAC GCAAAGAGGA AGAGGATGAT 
GGGGACCTGA ACAAAGCTCT GGGCGTCCAG CGCTTCCAGC AGATCCTCAG CCCTGCTTCT 
GTCGTACCTG ACGAGCAGCA CCACAACTAC CATGAGGAGG ACATCGAATA CCACCGTCAC 
TCCTCTCACC ACATCCACCG GCCTCTGTCC AAACTGCCCT CAGAGGGACG CAGGAAGAAG 
AGTGGCAAGA AGAGGAAGAA GGACAAAGAT CACAAAAGCA GCCACGTACC CAGCAGTGGC 
CCCATCGAGG AAGGAGAGGA TGAAGAGGAA GAGGAGGAGG AGGGGACGGA GGCAAGTTCT 
GCTCCCTCTG ACTCGGAGAG AGCCAAAGAT GTGGAGTTCT TTCTTTCGGA TGAAGACAGT 
GCAGCCAAAC GTGGCACAGA GAGCCCTCAC GCAGCCCGAG AGCTGGACAT CGTACCACAC 
ACTGGTGTCG GAGCAGATCC TGAGGATGTA ACTTCCACAG ATAAGCTGGA CACATCCGAC 
ACTTCCAGCC CCTCTCCTCC AGCAGTGCCA CCTGATCACA TACCGCTGGC CCGGGTGTCA 
TCCGCCAGCC GCAGCTACGA TCTGCAAGAC CGCCGGCGGA CGGGCAACAT GACGGGTGCT 
GAGCAGGCCA AGTACCAGCG TATCCCCACT GATGAGAGCG AGGCTCAGAC TCTGGCCTCC 
GCTGACCTGG ACGGCATCAA GAGTCATCGT TTTGAAGATG TCCCCGGCAT GCGCAGACAC 
CTGGTCAGAA AGAGTGCCAA GGGACAAGTG GTGCACATCA GTAAAGACCA CAAAGAGCCG 
ACCACCCGCA GCCGCAAGCA AGACCGAACC CCACATGAGG TGTTTGTAGA GCTGAACGAA 
CTGACCATGG ACAAGAACCA GGAGATGCAG TGGAAGGAAA CGGCTCGTTG GATCAAGTTT 
GAGGAGGATG TGGAGGAAGA GACGGACCGC TGGGGGAAAC CTCATGTGGC GTCTCTGTCT 
TTCCGCAGTT TGCTGGAGCT CAGAAGGACC ATCTCGCATG GTGCAGTGCT GCTGGACCTG 
GACCAGAAGA CCCTTCCTGG CATCGCACAT CAAGTGGTGG AGCAGATGAT CATTTCTGAT 
CAGATCAAAG CTGAAGACCG AGCCAACGTC CTGAGGGCCC TGCTGCTTAA ACACAGTCAC 
CCGAGCGATG AGAAAGAGCA CAGTAGCCCT TTCCCAAGGA ACATCTCGGC CGCCAGCCTC 
GGCAGCCTGA TGACACACCA CCACAGTGCC AACCACAGCC ATCAGCCCGA GCCGGCTGTC 
ACCGACCCGC TCATGGGCAC CGCCCATGCT GCGGGGGACA CGGACACACG CATCGACATG 
GAGAAGAACG ATGGACAGCA GAAGGAGCCG ACCTTGATGC CCGGCATGCA TCGATCCAAA 
TCCAAACATG AGCTGAAGCT GCTGGAGAAG ATTCCTGAAA ATGCAGAGGC CACAGTTGTC 
CTTGTGGGTA GTGTGGACTT CCTGGAGCAG CCCACCATGG CGTTTGTGCG GCTGCAGGAG 
GCAGTGGAGC TGGAGTCCGT CCTTGAAGTC CCTGTGCCTG TCAGGTTCCT TTTCGTTCTG 
CTGGGACCCC CCACCACGAG CATGGACTAC CACCAGATTG GACGCTCCAT CTCCACGCTG 
ATGTCTGACA AGCAATTCCA CGAGGCAGCC TATCTAGCAG ATGACAGGCA GGACCTGCTG 
AACGCAATCA ACAGCTTCCT GGACTGCAGC ATCGTGCTGC CGCCGTCGGA GATGGGAGGT 
GATGAGCTGC TGCGCTCCGT CGCTCGCTTC CAGCGGGAGA TGCTGCGCAA GAGGGCGGAG 
CAGGAGGTCA AACTGCTCAA AGAGCCTAAA AGCCTCGAAG AAAAAGAGGC CCTCCTCACA 
CCTCTGAAAA AGTCAGGCGA CCCTTTAGAG CGCACAGGAC GCCCCTTTGG TGGCCTGGTA 
CAAGACGTGC GGCGACGTTA CCCGAAGTAC GTCAGCGACT TCAAGGATGC CCTCAACAGT 
CAGTGCATGG CTGCGGTCAT CTTCATCTAC TTTGCTGCGC TCTCTCCAGC CATAACATTC 
GGAGGCCTGC TGGGTGAGAA GACGGGCGGT CTGATTGGTG TTTCTGAGCT GATTGTGTCA 
ACAGCGGTGC AGGGCGTGAT CTTCTGCCTG CTCGGAGCTC AGCCGCTGCT GGTCGTGGGC 
TTCTCCGGAC CCCTGCTAGT TTTTGAGGAA GCCTTTTACA GTTTCTGCAA AGCAAATGCG 
ATCGAGTACC TAACAGGCCG CGTCTGGATC GGGTTCTGGC TCATCATCAT CGTGGTCGTC 
ACTGTGGCGT TCGAAGGCAG TTTCCTGGTC CGCTTCGTCT CCCGCTTCAC CCAGGAGATC 
TTCTCCTTCC TCATCTCCCT CATCTTCATC TGCGAGACCT TCATCAAGCT CGGCAGGATT 
TTCAAGGAGC ACCCACTGAA ACGCTGCTCC CATGACAACA GCAGCACAAC GGGAAACTTC 
ACCCTGGATC TGGGCAACAG CAGCCTGTCT GTGAAGCCGA ATGTCCGAGA CGAGCCCAAC 
ACGGCGCTGC TCTCTCTGGT CCTCATGGCC GGGACATTTT TCATCGCTTT CTACCTGCGC 
AAGTTCAAGA ACAGTGCATT CTTCCCTGGA AGGCTTCGCA GGGTTATTGG AGATTTCGGG 
GTTCCCATCG CCATCCTCAT CATGGTGCTG GTAGATTACA GCATAGAAGA CACGTACACA 
CAAAAACTGA GTGTCCCCAG TGGACTTTCT GTGACGAGTC CCGACAAACG CGGTTGGATC 
ATCAACCCTC TGGGTTCGGA CGGTAAATTC CCCATCTGGA TGATGTTCGC CTGCTGTTTG 
CCCGCTCTGC TGGTTTTCAT CCTCATCTTC ATGGAGACGC AGATCACCAC GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019962912.1
CDS	295..3288
Translation	MSNPSVPNQITDAMAAVIHSPEAPAASSLHGTPRKEEEDDGDLNKALGVQRFQQILSPASVVPDEQHHNYHEEDIEYHRHSSHHIHRPLSKLPSEGRRKKSGKKRKKDKDHKSSHVPSSGPIEEGEDEEEEEEEGTEASSAPSDSERAKDVEFFLSDEDSAAKRGTESPHAARELDIVPHTGVGADPEDVTSTDKLDTSDTSSPSPPAVPPDHIPLARVSSASRSYDLQDRRRTGNMTGAEQAKYQRIPTDESEAQTLASADLDGIKSHRFEDVPGMRRHLVRKSAKGQVVHISKDHKEPTTRSRKQDRTPHEVFVELNELTMDKNQEMQWKETARWIKFEEDVEEETDRWGKPHVASLSFRSLLELRRTISHGAVLLDLDQKTLPGIAHQVVEQMIISDQIKAEDRANVLRALLLKHSHPSDEKEHSSPFPRNISAASLGSLMTHHHSANHSHQPEPAVTDPLMGTAHAAGDTDTRIDMEKNDGQQKEPTLMPGMHRSKSKHELKLLEKIPENAEATVVLVGSVDFLEQPTMAFVRLQEAVELESVLEVPVPVRFLFVLLGPPTTSMDYHQIGRSISTLMSDKQFHEAAYLADDRQDLLNAINSFLDCSIVLPPSEMGGDELLRSVARFQREMLRKRAEQEVKLLKEPKSLEEKEALLTPLKKSGDPLERTGRPFGGLVQDVRRRYPKYVSDFKDALNSQCMAAVIFIYFAALSPAITFGGLLGEKTGGLIGVSELIVSTAVQGVIFCLLGAQPLLVVGFSGPLLVFEEAFYSFCKANAIEYLTGRVWIGFWLIIIVVVTVAFEGSFLVRFVSRFTQEIFSFLISLIFICETFIKLGRIFKEHPLKRCSHDNSSTTGNFTLDLGNSSLSVKPNVRDEPNTALLSLVLMAGTFFIAFYLRKFKNSAFFPGRLRRVIGDFGVPIAILIMVLVDYSIEDTYTQKLSVPSGLSVTSPDKRGWIINPLGSDGKFPIWMMFACCLPALLVFILIFMETQITT

Target ORF information:

RefSeq Version	XM_020107353.1
Organism	Paralichthys olivaceus(Japanese flounder)
Definition	Paralichthys olivaceus anion exchange protein 2-like (LOC109642528), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_020107353.1

ORF Insert Sequence:

ATGAGCAACC CCAGCGTTCC CAACCAGATT ACAGATGCCA TGGCAGCGGT CATTCACAGT 
CCAGAGGCTC CTGCAGCCTC ATCACTACAT GGGACTCCAC GCAAAGAGGA AGAGGATGAT 
GGGGACCTGA ACAAAGCTCT GGGCGTCCAG CGCTTCCAGC AGATCCTCAG CCCTGCTTCT 
GTCGTACCTG ACGAGCAGCA CCACAACTAC CATGAGGAGG ACATCGAATA CCACCGTCAC 
TCCTCTCACC ACATCCACCG GCCTCTGTCC AAACTGCCCT CAGAGGGACG CAGGAAGAAG 
AGTGGCAAGA AGAGGAAGAA GGACAAAGAT CACAAAAGCA GCCACGTACC CAGCAGTGGC 
CCCATCGAGG AAGGAGAGGA TGAAGAGGAA GAGGAGGAGG AGGGGACGGA GGCAAGTTCT 
GCTCCCTCTG ACTCGGAGAG AGCCAAAGAT GTGGAGTTCT TTCTTTCGGA TGAAGACAGT 
GCAGCCAAAC GTGGCACAGA GAGCCCTCAC GCAGCCCGAG AGCTGGACAT CGTACCACAC 
ACTGGTGTCG GAGCAGATCC TGAGGATGTA ACTTCCACAG ATAAGCTGGA CACATCCGAC 
ACTTCCAGCC CCTCTCCTCC AGCAGTGCCA CCTGATCACA TACCGCTGGC CCGGGTGTCA 
TCCGCCAGCC GCAGCTACGA TCTGCAAGAC CGCCGGCGGA CGGGCAACAT GACGGGTGCT 
GAGCAGGCCA AGTACCAGCG TATCCCCACT GATGAGAGCG AGGCTCAGAC TCTGGCCTCC 
GCTGACCTGG ACGGCATCAA GAGTCATCGT TTTGAAGATG TCCCCGGCAT GCGCAGACAC 
CTGGTCAGAA AGAGTGCCAA GGGACAAGTG GTGCACATCA GTAAAGACCA CAAAGAGCCG 
ACCACCCGCA GCCGCAAGCA AGACCGAACC CCACATGAGG TGTTTGTAGA GCTGAACGAA 
CTGACCATGG ACAAGAACCA GGAGATGCAG TGGAAGGAAA CGGCTCGTTG GATCAAGTTT 
GAGGAGGATG TGGAGGAAGA GACGGACCGC TGGGGGAAAC CTCATGTGGC GTCTCTGTCT 
TTCCGCAGTT TGCTGGAGCT CAGAAGGACC ATCTCGCATG GTGCAGTGCT GCTGGACCTG 
GACCAGAAGA CCCTTCCTGG CATCGCACAT CAAGTGGTGG AGCAGATGAT CATTTCTGAT 
CAGATCAAAG CTGAAGACCG AGCCAACGTC CTGAGGGCCC TGCTGCTTAA ACACAGTCAC 
CCGAGCGATG AGAAAGAGCA CAGTAGCCCT TTCCCAAGGA ACATCTCGGC CGCCAGCCTC 
GGCAGCCTGA TGACACACCA CCACAGTGCC AACCACAGCC ATCAGCCCGA GCCGGCTGTC 
ACCGACCCGC TCATGGGCAC CGCCCATGCT GCGGGGGACA CGGACACACG CATCGACATG 
GAGAAGAACG ATGGACAGCA GAAGGAGCCG ACCTTGATGC CCGGCATGCA TCGATCCAAA 
TCCAAACATG AGCTGAAGCT GCTGGAGAAG ATTCCTGAAA ATGCAGAGGC CACAGTTGTC 
CTTGTGGGTA GTGTGGACTT CCTGGAGCAG CCCACCATGG CGTTTGTGCG GCTGCAGGAG 
GCAGTGGAGC TGGAGTCCGT CCTTGAAGTC CCTGTGCCTG TCAGGTTCCT TTTCGTTCTG 
CTGGGACCCC CCACCACGAG CATGGACTAC CACCAGATTG GACGCTCCAT CTCCACGCTG 
ATGTCTGACA AGCAATTCCA CGAGGCAGCC TATCTAGCAG ATGACAGGCA GGACCTGCTG 
AACGCAATCA ACAGCTTCCT GGACTGCAGC ATCGTGCTGC CGCCGTCGGA GATGGGAGGT 
GATGAGCTGC TGCGCTCCGT CGCTCGCTTC CAGCGGGAGA TGCTGCGCAA GAGGGCGGAG 
CAGGAGGTCA AACTGCTCAA AGAGCCTAAA AGCCTCGAAG AAAAAGAGGC CCTCCTCACA 
CCTCTGAAAA AGTCAGGCGA CCCTTTAGAG CGCACAGGAC GCCCCTTTGG TGGCCTGGTA 
CAAGACGTGC GGCGACGTTA CCCGAAGTAC GTCAGCGACT TCAAGGATGC CCTCAACAGT 
CAGTGCATGG CTGCGGTCAT CTTCATCTAC TTTGCTGCGC TCTCTCCAGC CATAACATTC 
GGAGGCCTGC TGGGTGAGAA GACGGGCGGT CTGATTGGTG TTTCTGAGCT GATTGTGTCA 
ACAGCGGTGC AGGGCGTGAT CTTCTGCCTG CTCGGAGCTC AGCCGCTGCT GGTCGTGGGC 
TTCTCCGGAC CCCTGCTAGT TTTTGAGGAA GCCTTTTACA GTTTCTGCAA AGCAAATGCG 
ATCGAGTACC TAACAGGCCG CGTCTGGATC GGGTTCTGGC TCATCATCAT CGTGGTCGTC 
ACTGTGGCGT TCGAAGGCAG TTTCCTGGTC CGCTTCGTCT CCCGCTTCAC CCAGGAGATC 
TTCTCCTTCC TCATCTCCCT CATCTTCATC TGCGAGACCT TCATCAAGCT CGGCAGGATT 
TTCAAGGAGC ACCCACTGAA ACGCTGCTCC CATGACAACA GCAGCACAAC GGGAAACTTC 
ACCCTGGATC TGGGCAACAG CAGCCTGTCT GTGAAGCCGA ATGTCCGAGA CGAGCCCAAC 
ACGGCGCTGC TCTCTCTGGT CCTCATGGCC GGGACATTTT TCATCGCTTT CTACCTGCGC 
AAGTTCAAGA ACAGTGCATT CTTCCCTGGA AGGCTTCGCA GGGTTATTGG AGATTTCGGG 
GTTCCCATCG CCATCCTCAT CATGGTGCTG GTAGATTACA GCATAGAAGA CACGTACACA 
CAAAAACTGA GTGTCCCCAG TGGACTTTCT GTGACGAGTC CCGACAAACG CGGTTGGATC 
ATCAACCCTC TGGGTTCGGA CGGTAAATTC CCCATCTGGA TGATGTTCGC CTGCTGTTTG 
CCCGCTCTGC TGGTTTTCAT CCTCATCTTC ATGGAGACGC AGATCACCAC GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC109642528 ( XM_020107354.1 ) cDNA ORF clone, Paralichthys olivaceus(Japanese flounder) -> XP_019962913.1 Paralichthys olivaceus anion exchange protein 2-like (LOC109642528), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OPa256192
Clone ID Related Accession (Same CDS sequence)	XM_020107354.1
Accession Version	XM_020107354.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2991bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-02-07
Organism	Paralichthys olivaceus(Japanese flounder)
Product	anion exchange protein 2-like isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_017860137.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Paralichthys olivaceus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCAACC CCAGCGTTCC CAACCAGATT ACAGATGCCA TGGCAGCGGT CATTCACAGT 
CCAGAGGCTC CTGCAGCCTC ATCACTACAT GGGACTCCAC GCAAAGAGGA AGAGGATGAT 
GGGGACCTGA ACAAAGCTCT GGGCGTCCAG CGCTTCCAGC AGATCCTCAG CCCTGCTTCT 
GTCGTACCTG ACGAGCAGCA CCACAACTAC CATGAGGAGG ACATCGAATA CCACCGTCAC 
TCCTCTCACC ACATCCACCG GCCTCTGTCC AAACTGCCCT CAGAGGGACG CAGGAAGAAG 
AGTGGCAAGA AGAGGAAGAA GGACAAAGAT CACAAAAGCA GCCACGTACC CAGCAGTGGC 
CCCATCGAGG AAGGAGAGGA TGAAGAGGAA GAGGAGGAGG AGGGGACGGA GGCAAGTTCT 
GCTCCCTCTG ACTCGGAGAG AGCCAAAGAT GTGGAGTTCT TTCTTTCGGA TGAAGACAGT 
GCAGCCAAAC GTGGCACAGA GAGCCCTCAC GCAGCCCGAG AGCTGGACAT CGTACCACAC 
ACTGGTGTCG GAGCAGATCC TGAGGATGTA ACTTCCACAG ATAAGCTGGA CACATCCGAC 
ACTTCCAGCC CCTCTCCTCC AGCAGTGCCA CCTGATCACA TACCGCTGGC CCGGGTGTCA 
TCCGCCAGCC GCAGCTACGA TCTGCAAGAC CGCCGGCGGA CGGGCAACAT GACGGGTGCT 
GAGCAGGCCA AGTACCAGCG TATCCCCACT GATGAGAGCG AGGCTCAGAC TCTGGCCTCC 
GCTGACCTGG ACGGCATCAA GAGTCATCGT TTTGAAGATG TCCCCGGCAT GCGCAGACAC 
CTGGTCAGAA AGAGTGCCAA GGGACAAGTG GTGCACATCA GTAAAGACCA CAAAGAGCCG 
ACCACCCGCA GCCGCAAGCA AGACCGAACC CCACATGAGG TGTTTGTAGA GCTGAACGAA 
CTGACCATGG ACAAGAACCA GGAGATGCAG TGGAAGGAAA CGGCTCGTTG GATCAAGTTT 
GAGGAGGATG TGGAGGAAGA GACGGACCGC TGGGGGAAAC CTCATGTGGC GTCTCTGTCT 
TTCCGCAGTT TGCTGGAGCT CAGAAGGACC ATCTCGCATG GTGCAGTGCT GCTGGACCTG 
GACCAGAAGA CCCTTCCTGG CATCGCACAT CAAGTGGTGG AGCAGATGAT CATTTCTGAT 
CAGATCAAAG CTGAAGACCG AGCCAACGTC CTGAGGGCCC TGCTGCTTAA ACACAGTCAC 
CCGAGCGATG AGAAAGAGCA CAGTAGCCCT TTCCCAAGGA ACATCTCGGC CGCCAGCCTC 
GGCAGCCTGA TGACACACCA CCACAGTGCC AACCACAGCC ATCAGCCCGA GCCGGCTGTC 
ACCGACCCGC TCATGGGCAC CGCCCATGCT GCGGGGGACA CGGACACACG CATCGACATG 
GAGAAGAACG ATGGACAGAA GGAGCCGACC TTGATGCCCG GCATGCATCG ATCCAAATCC 
AAACATGAGC TGAAGCTGCT GGAGAAGATT CCTGAAAATG CAGAGGCCAC AGTTGTCCTT 
GTGGGTAGTG TGGACTTCCT GGAGCAGCCC ACCATGGCGT TTGTGCGGCT GCAGGAGGCA 
GTGGAGCTGG AGTCCGTCCT TGAAGTCCCT GTGCCTGTCA GGTTCCTTTT CGTTCTGCTG 
GGACCCCCCA CCACGAGCAT GGACTACCAC CAGATTGGAC GCTCCATCTC CACGCTGATG 
TCTGACAAGC AATTCCACGA GGCAGCCTAT CTAGCAGATG ACAGGCAGGA CCTGCTGAAC 
GCAATCAACA GCTTCCTGGA CTGCAGCATC GTGCTGCCGC CGTCGGAGAT GGGAGGTGAT 
GAGCTGCTGC GCTCCGTCGC TCGCTTCCAG CGGGAGATGC TGCGCAAGAG GGCGGAGCAG 
GAGGTCAAAC TGCTCAAAGA GCCTAAAAGC CTCGAAGAAA AAGAGGCCCT CCTCACACCT 
CTGAAAAAGT CAGGCGACCC TTTAGAGCGC ACAGGACGCC CCTTTGGTGG CCTGGTACAA 
GACGTGCGGC GACGTTACCC GAAGTACGTC AGCGACTTCA AGGATGCCCT CAACAGTCAG 
TGCATGGCTG CGGTCATCTT CATCTACTTT GCTGCGCTCT CTCCAGCCAT AACATTCGGA 
GGCCTGCTGG GTGAGAAGAC GGGCGGTCTG ATTGGTGTTT CTGAGCTGAT TGTGTCAACA 
GCGGTGCAGG GCGTGATCTT CTGCCTGCTC GGAGCTCAGC CGCTGCTGGT CGTGGGCTTC 
TCCGGACCCC TGCTAGTTTT TGAGGAAGCC TTTTACAGTT TCTGCAAAGC AAATGCGATC 
GAGTACCTAA CAGGCCGCGT CTGGATCGGG TTCTGGCTCA TCATCATCGT GGTCGTCACT 
GTGGCGTTCG AAGGCAGTTT CCTGGTCCGC TTCGTCTCCC GCTTCACCCA GGAGATCTTC 
TCCTTCCTCA TCTCCCTCAT CTTCATCTGC GAGACCTTCA TCAAGCTCGG CAGGATTTTC 
AAGGAGCACC CACTGAAACG CTGCTCCCAT GACAACAGCA GCACAACGGG AAACTTCACC 
CTGGATCTGG GCAACAGCAG CCTGTCTGTG AAGCCGAATG TCCGAGACGA GCCCAACACG 
GCGCTGCTCT CTCTGGTCCT CATGGCCGGG ACATTTTTCA TCGCTTTCTA CCTGCGCAAG 
TTCAAGAACA GTGCATTCTT CCCTGGAAGG CTTCGCAGGG TTATTGGAGA TTTCGGGGTT 
CCCATCGCCA TCCTCATCAT GGTGCTGGTA GATTACAGCA TAGAAGACAC GTACACACAA 
AAACTGAGTG TCCCCAGTGG ACTTTCTGTG ACGAGTCCCG ACAAACGCGG TTGGATCATC 
AACCCTCTGG GTTCGGACGG TAAATTCCCC ATCTGGATGA TGTTCGCCTG CTGTTTGCCC 
GCTCTGCTGG TTTTCATCCT CATCTTCATG GAGACGCAGA TCACCACGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019962913.1
CDS	295..3285
Translation	MSNPSVPNQITDAMAAVIHSPEAPAASSLHGTPRKEEEDDGDLNKALGVQRFQQILSPASVVPDEQHHNYHEEDIEYHRHSSHHIHRPLSKLPSEGRRKKSGKKRKKDKDHKSSHVPSSGPIEEGEDEEEEEEEGTEASSAPSDSERAKDVEFFLSDEDSAAKRGTESPHAARELDIVPHTGVGADPEDVTSTDKLDTSDTSSPSPPAVPPDHIPLARVSSASRSYDLQDRRRTGNMTGAEQAKYQRIPTDESEAQTLASADLDGIKSHRFEDVPGMRRHLVRKSAKGQVVHISKDHKEPTTRSRKQDRTPHEVFVELNELTMDKNQEMQWKETARWIKFEEDVEEETDRWGKPHVASLSFRSLLELRRTISHGAVLLDLDQKTLPGIAHQVVEQMIISDQIKAEDRANVLRALLLKHSHPSDEKEHSSPFPRNISAASLGSLMTHHHSANHSHQPEPAVTDPLMGTAHAAGDTDTRIDMEKNDGQKEPTLMPGMHRSKSKHELKLLEKIPENAEATVVLVGSVDFLEQPTMAFVRLQEAVELESVLEVPVPVRFLFVLLGPPTTSMDYHQIGRSISTLMSDKQFHEAAYLADDRQDLLNAINSFLDCSIVLPPSEMGGDELLRSVARFQREMLRKRAEQEVKLLKEPKSLEEKEALLTPLKKSGDPLERTGRPFGGLVQDVRRRYPKYVSDFKDALNSQCMAAVIFIYFAALSPAITFGGLLGEKTGGLIGVSELIVSTAVQGVIFCLLGAQPLLVVGFSGPLLVFEEAFYSFCKANAIEYLTGRVWIGFWLIIIVVVTVAFEGSFLVRFVSRFTQEIFSFLISLIFICETFIKLGRIFKEHPLKRCSHDNSSTTGNFTLDLGNSSLSVKPNVRDEPNTALLSLVLMAGTFFIAFYLRKFKNSAFFPGRLRRVIGDFGVPIAILIMVLVDYSIEDTYTQKLSVPSGLSVTSPDKRGWIINPLGSDGKFPIWMMFACCLPALLVFILIFMETQITT

Target ORF information:

RefSeq Version	XM_020107354.1
Organism	Paralichthys olivaceus(Japanese flounder)
Definition	Paralichthys olivaceus anion exchange protein 2-like (LOC109642528), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_020107354.1

ORF Insert Sequence:

ATGAGCAACC CCAGCGTTCC CAACCAGATT ACAGATGCCA TGGCAGCGGT CATTCACAGT 
CCAGAGGCTC CTGCAGCCTC ATCACTACAT GGGACTCCAC GCAAAGAGGA AGAGGATGAT 
GGGGACCTGA ACAAAGCTCT GGGCGTCCAG CGCTTCCAGC AGATCCTCAG CCCTGCTTCT 
GTCGTACCTG ACGAGCAGCA CCACAACTAC CATGAGGAGG ACATCGAATA CCACCGTCAC 
TCCTCTCACC ACATCCACCG GCCTCTGTCC AAACTGCCCT CAGAGGGACG CAGGAAGAAG 
AGTGGCAAGA AGAGGAAGAA GGACAAAGAT CACAAAAGCA GCCACGTACC CAGCAGTGGC 
CCCATCGAGG AAGGAGAGGA TGAAGAGGAA GAGGAGGAGG AGGGGACGGA GGCAAGTTCT 
GCTCCCTCTG ACTCGGAGAG AGCCAAAGAT GTGGAGTTCT TTCTTTCGGA TGAAGACAGT 
GCAGCCAAAC GTGGCACAGA GAGCCCTCAC GCAGCCCGAG AGCTGGACAT CGTACCACAC 
ACTGGTGTCG GAGCAGATCC TGAGGATGTA ACTTCCACAG ATAAGCTGGA CACATCCGAC 
ACTTCCAGCC CCTCTCCTCC AGCAGTGCCA CCTGATCACA TACCGCTGGC CCGGGTGTCA 
TCCGCCAGCC GCAGCTACGA TCTGCAAGAC CGCCGGCGGA CGGGCAACAT GACGGGTGCT 
GAGCAGGCCA AGTACCAGCG TATCCCCACT GATGAGAGCG AGGCTCAGAC TCTGGCCTCC 
GCTGACCTGG ACGGCATCAA GAGTCATCGT TTTGAAGATG TCCCCGGCAT GCGCAGACAC 
CTGGTCAGAA AGAGTGCCAA GGGACAAGTG GTGCACATCA GTAAAGACCA CAAAGAGCCG 
ACCACCCGCA GCCGCAAGCA AGACCGAACC CCACATGAGG TGTTTGTAGA GCTGAACGAA 
CTGACCATGG ACAAGAACCA GGAGATGCAG TGGAAGGAAA CGGCTCGTTG GATCAAGTTT 
GAGGAGGATG TGGAGGAAGA GACGGACCGC TGGGGGAAAC CTCATGTGGC GTCTCTGTCT 
TTCCGCAGTT TGCTGGAGCT CAGAAGGACC ATCTCGCATG GTGCAGTGCT GCTGGACCTG 
GACCAGAAGA CCCTTCCTGG CATCGCACAT CAAGTGGTGG AGCAGATGAT CATTTCTGAT 
CAGATCAAAG CTGAAGACCG AGCCAACGTC CTGAGGGCCC TGCTGCTTAA ACACAGTCAC 
CCGAGCGATG AGAAAGAGCA CAGTAGCCCT TTCCCAAGGA ACATCTCGGC CGCCAGCCTC 
GGCAGCCTGA TGACACACCA CCACAGTGCC AACCACAGCC ATCAGCCCGA GCCGGCTGTC 
ACCGACCCGC TCATGGGCAC CGCCCATGCT GCGGGGGACA CGGACACACG CATCGACATG 
GAGAAGAACG ATGGACAGAA GGAGCCGACC TTGATGCCCG GCATGCATCG ATCCAAATCC 
AAACATGAGC TGAAGCTGCT GGAGAAGATT CCTGAAAATG CAGAGGCCAC AGTTGTCCTT 
GTGGGTAGTG TGGACTTCCT GGAGCAGCCC ACCATGGCGT TTGTGCGGCT GCAGGAGGCA 
GTGGAGCTGG AGTCCGTCCT TGAAGTCCCT GTGCCTGTCA GGTTCCTTTT CGTTCTGCTG 
GGACCCCCCA CCACGAGCAT GGACTACCAC CAGATTGGAC GCTCCATCTC CACGCTGATG 
TCTGACAAGC AATTCCACGA GGCAGCCTAT CTAGCAGATG ACAGGCAGGA CCTGCTGAAC 
GCAATCAACA GCTTCCTGGA CTGCAGCATC GTGCTGCCGC CGTCGGAGAT GGGAGGTGAT 
GAGCTGCTGC GCTCCGTCGC TCGCTTCCAG CGGGAGATGC TGCGCAAGAG GGCGGAGCAG 
GAGGTCAAAC TGCTCAAAGA GCCTAAAAGC CTCGAAGAAA AAGAGGCCCT CCTCACACCT 
CTGAAAAAGT CAGGCGACCC TTTAGAGCGC ACAGGACGCC CCTTTGGTGG CCTGGTACAA 
GACGTGCGGC GACGTTACCC GAAGTACGTC AGCGACTTCA AGGATGCCCT CAACAGTCAG 
TGCATGGCTG CGGTCATCTT CATCTACTTT GCTGCGCTCT CTCCAGCCAT AACATTCGGA 
GGCCTGCTGG GTGAGAAGAC GGGCGGTCTG ATTGGTGTTT CTGAGCTGAT TGTGTCAACA 
GCGGTGCAGG GCGTGATCTT CTGCCTGCTC GGAGCTCAGC CGCTGCTGGT CGTGGGCTTC 
TCCGGACCCC TGCTAGTTTT TGAGGAAGCC TTTTACAGTT TCTGCAAAGC AAATGCGATC 
GAGTACCTAA CAGGCCGCGT CTGGATCGGG TTCTGGCTCA TCATCATCGT GGTCGTCACT 
GTGGCGTTCG AAGGCAGTTT CCTGGTCCGC TTCGTCTCCC GCTTCACCCA GGAGATCTTC 
TCCTTCCTCA TCTCCCTCAT CTTCATCTGC GAGACCTTCA TCAAGCTCGG CAGGATTTTC 
AAGGAGCACC CACTGAAACG CTGCTCCCAT GACAACAGCA GCACAACGGG AAACTTCACC 
CTGGATCTGG GCAACAGCAG CCTGTCTGTG AAGCCGAATG TCCGAGACGA GCCCAACACG 
GCGCTGCTCT CTCTGGTCCT CATGGCCGGG ACATTTTTCA TCGCTTTCTA CCTGCGCAAG 
TTCAAGAACA GTGCATTCTT CCCTGGAAGG CTTCGCAGGG TTATTGGAGA TTTCGGGGTT 
CCCATCGCCA TCCTCATCAT GGTGCTGGTA GATTACAGCA TAGAAGACAC GTACACACAA 
AAACTGAGTG TCCCCAGTGG ACTTTCTGTG ACGAGTCCCG ACAAACGCGG TTGGATCATC 
AACCCTCTGG GTTCGGACGG TAAATTCCCC ATCTGGATGA TGTTCGCCTG CTGTTTGCCC 
GCTCTGCTGG TTTTCATCCT CATCTTCATG GAGACGCAGA TCACCACGTG A