il12b cDNA ORF clone, Paralichthys olivaceus(Japanese flounder)

Gene
Clones
gRNAs

Summary

Gene Symbol	il12b
Entrez Gene ID	109624692
Full Name	interleukin 12B
Gene Type	protein-coding
Organism	Paralichthys olivaceus(Japanese flounder)

mRNA and Protein(s)

mRNA	Protein	Name
XM_020079566.1	XP_019935125.1	interleukin-12 subunit beta isoform X2
XM_020079557.1	XP_019935116.1	interleukin-12 subunit beta isoform X1

Pathways from BioSystems
Gene Ontology

The following il12b gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the il12b cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OPa230696	XM_020079557.1 Latest version!	Paralichthys olivaceus interleukin 12B (il12b), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$258.30 $369.00
OPa230697	XM_020079566.1 Latest version!	Paralichthys olivaceus interleukin 12B (il12b), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$174.30 $249.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

il12b ( XM_020079557.1 ) cDNA ORF clone, Paralichthys olivaceus(Japanese flounder) -> XP_019935116.1 Paralichthys olivaceus interleukin 12B (il12b), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OPa230696
Clone ID Related Accession (Same CDS sequence)	XM_020079557.1
Accession Version	XM_020079557.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1074bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-02-07
Organism	Paralichthys olivaceus(Japanese flounder)
Product	interleukin-12 subunit beta isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_017859641.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Paralichthys olivaceus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGTTCT TTACTTGCAG CGTCATCATG TCTGTATTTC TACAAGTCTG CAGCCAAAAT 
CCTCTGCGCT ATTGGACTCT GCTCCCCAAC ATTCTGGTTG TGGAGGTGAA CGGCTCTCTG 
GGCCAGCAGC CCCTGAGCTG CTTGGGCTCG CCACAGGAGG GGACGAGGAA AGACGACAAG 
AGTCAGGACA TTATTTGGAG GAAGAATGGA GTAGTGGAAG AACAGAGGGG AAACTCGTAC 
TTGGTGCAGC TGGAGGAAAG TTTAGGTGGG GGCAACTACA CATGCCACAG AGAGGACGGA 
TCACTGGTCA ATCACACTGT GGTTCTGATA CAGGAGGACC AAACCGAGAG GAGGAAGATT 
CTTGTGAAAA CTGATCAAGA GGATTACTTG ACGTGCTTGG CTCAAAATTA CAACGGAGAG 
TTTCACTGCT CTTGGACCTG GAACAGAAAT CGTGTTGGCA AAGTAGCATT GATCCAGGTT 
GGGCGCATTT CTAACGACAG TGACGCCCAG TGTTCTGTGG ACGTCAGTAC CCAGCGCTGG 
ACGTGCTCCT CAGGTCAGAG TAACTTCAGC TGCTCAGTCG ATGAACATGG ACACAGGATC 
TCCTGCCGAG ACGAGCAGCA CTGCCCCTAC GCTGAGGAGA TCCAGCAGAT CTACGTCACT 
TTGTACGTGA GGACCGAGCA ATTCTTGGTG GAGAACTACT CTAAACAGTT TTACCTGGCA 
GAGATAGTGA AACCTGACAT GGTGAGGATA AGTGAGGTCA ACACCTCAAT GATTGAGTGG 
AGTTACCCGA GCTCGTGGAG CAGTCCCTAC TCCTACTTCC CTCTTACTTT CCAGATCTCA 
CAGTTCAGGC GGGGATGCAA AGGGTGTGAC AACCCCTGCA CTGAGTCAAA ACCTGCAAAG 
ACCCTGATGG TCCATTTCAC AGACGTTTGC CAATTTAAAG TCAAGCACAA GGCCAAGGCT 
GTGTGTGTCC GAGCAAAGGA TGCTCTCTGT AACTCCCAGT GGAGCGAATG GAGCCACCTC 
AGATTGAGGA GAAACAATAA GAACAAACAC CGGCAAAATG ATAAACAAGG GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019935116.1
CDS	1..1074
Translation	MKFFTCSVIMSVFLQVCSQNPLRYWTLLPNILVVEVNGSLGQQPLSCLGSPQEGTRKDDKSQDIIWRKNGVVEEQRGNSYLVQLEESLGGGNYTCHREDGSLVNHTVVLIQEDQTERRKILVKTDQEDYLTCLAQNYNGEFHCSWTWNRNRVGKVALIQVGRISNDSDAQCSVDVSTQRWTCSSGQSNFSCSVDEHGHRISCRDEQHCPYAEEIQQIYVTLYVRTEQFLVENYSKQFYLAEIVKPDMVRISEVNTSMIEWSYPSSWSSPYSYFPLTFQISQFRRGCKGCDNPCTESKPAKTLMVHFTDVCQFKVKHKAKAVCVRAKDALCNSQWSEWSHLRLRRNNKNKHRQNDKQG

Target ORF information:

RefSeq Version	XM_020079557.1
Organism	Paralichthys olivaceus(Japanese flounder)
Definition	Paralichthys olivaceus interleukin 12B (il12b), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_020079557.1

ORF Insert Sequence:

ATGAAGTTCT TTACTTGCAG CGTCATCATG TCTGTATTTC TACAAGTCTG CAGCCAAAAT 
CCTCTGCGCT ATTGGACTCT GCTCCCCAAC ATTCTGGTTG TGGAGGTGAA CGGCTCTCTG 
GGCCAGCAGC CCCTGAGCTG CTTGGGCTCG CCACAGGAGG GGACGAGGAA AGACGACAAG 
AGTCAGGACA TTATTTGGAG GAAGAATGGA GTAGTGGAAG AACAGAGGGG AAACTCGTAC 
TTGGTGCAGC TGGAGGAAAG TTTAGGTGGG GGCAACTACA CATGCCACAG AGAGGACGGA 
TCACTGGTCA ATCACACTGT GGTTCTGATA CAGGAGGACC AAACCGAGAG GAGGAAGATT 
CTTGTGAAAA CTGATCAAGA GGATTACTTG ACGTGCTTGG CTCAAAATTA CAACGGAGAG 
TTTCACTGCT CTTGGACCTG GAACAGAAAT CGTGTTGGCA AAGTAGCATT GATCCAGGTT 
GGGCGCATTT CTAACGACAG TGACGCCCAG TGTTCTGTGG ACGTCAGTAC CCAGCGCTGG 
ACGTGCTCCT CAGGTCAGAG TAACTTCAGC TGCTCAGTCG ATGAACATGG ACACAGGATC 
TCCTGCCGAG ACGAGCAGCA CTGCCCCTAC GCTGAGGAGA TCCAGCAGAT CTACGTCACT 
TTGTACGTGA GGACCGAGCA ATTCTTGGTG GAGAACTACT CTAAACAGTT TTACCTGGCA 
GAGATAGTGA AACCTGACAT GGTGAGGATA AGTGAGGTCA ACACCTCAAT GATTGAGTGG 
AGTTACCCGA GCTCGTGGAG CAGTCCCTAC TCCTACTTCC CTCTTACTTT CCAGATCTCA 
CAGTTCAGGC GGGGATGCAA AGGGTGTGAC AACCCCTGCA CTGAGTCAAA ACCTGCAAAG 
ACCCTGATGG TCCATTTCAC AGACGTTTGC CAATTTAAAG TCAAGCACAA GGCCAAGGCT 
GTGTGTGTCC GAGCAAAGGA TGCTCTCTGT AACTCCCAGT GGAGCGAATG GAGCCACCTC 
AGATTGAGGA GAAACAATAA GAACAAACAC CGGCAAAATG ATAAACAAGG GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

il12b ( XM_020079566.1 ) cDNA ORF clone, Paralichthys olivaceus(Japanese flounder) -> XP_019935125.1 Paralichthys olivaceus interleukin 12B (il12b), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OPa230697
Clone ID Related Accession (Same CDS sequence)	XM_020079566.1
Accession Version	XM_020079566.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 954bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-02-07
Organism	Paralichthys olivaceus(Japanese flounder)
Product	interleukin-12 subunit beta isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_017859641.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Paralichthys olivaceus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGTAT TTCTACAAGT CTGCAGCCAA AATCCTCTGC GCTATTGGAC TCTGCTCCCC 
AACATTCTGG TTGTGGAGGT GAACGGCTCT CTGGGCCAGC AGCCCCTGAG CTGCTTGGGC 
TCGCCACAGG AGGGGACGAG GAAAGACGAC AAGAGTCAGG ACATTATTTG GAGGAAGAAT 
GGAGTAGTGG AAGAACAGAG GGGAAACTCG TACTTGGTGC AGCTGGAGGA AAGTTTAGGT 
GGGGGCAACT ACACATGCCA CAGAGAGGAC GGATCACTGG TCAATCACAC TGTGGTTCTG 
ATACAGGAGG ACCAAACCGA GAGGAGGAAG ATTCTTGTGA AAACTGATCA AGAGGATTAC 
TTGACGTGCT TGGCTCAAAA TTACAACGGA GAGTTTCACT GCTCTTGGAC CTGGAACAGA 
AATCGTGTTG GCAAAGTAGC ATTGATCCAG GTTGGGCGCA TTTCTAACGA CAGTGACGCC 
CAGTGTTCTG TGGACGTCAG TACCCAGCGC TGGACGTGCT CCTCAGGTCA GAGTAACTTC 
AGCTGCTCAG TCGATGAACA TGGACACAGG ATCTCCTGCC GAGACGAGCA GCACTGCCCC 
TACGCTGAGG AGATCCAGCA GATCTACGTC ACTTTGTACG TGAGGACCGA GCAATTCTTG 
GTGGAGAACT ACTCTAAACA GTTTTACCTG GCAGAGATAG TGAAACCTGA CATGATCTCA 
CAGTTCAGGC GGGGATGCAA AGGGTGTGAC AACCCCTGCA CTGAGTCAAA ACCTGCAAAG 
ACCCTGATGG TCCATTTCAC AGACGTTTGC CAATTTAAAG TCAAGCACAA GGCCAAGGCT 
GTGTGTGTCC GAGCAAAGGA TGCTCTCTGT AACTCCCAGT GGAGCGAATG GAGCCACCTC 
AGATTGAGGA GAAACAATAA GAACAAACAC CGGCAAAATG ATAAACAAGG GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019935125.1
CDS	24..977
Translation	MSVFLQVCSQNPLRYWTLLPNILVVEVNGSLGQQPLSCLGSPQEGTRKDDKSQDIIWRKNGVVEEQRGNSYLVQLEESLGGGNYTCHREDGSLVNHTVVLIQEDQTERRKILVKTDQEDYLTCLAQNYNGEFHCSWTWNRNRVGKVALIQVGRISNDSDAQCSVDVSTQRWTCSSGQSNFSCSVDEHGHRISCRDEQHCPYAEEIQQIYVTLYVRTEQFLVENYSKQFYLAEIVKPDMISQFRRGCKGCDNPCTESKPAKTLMVHFTDVCQFKVKHKAKAVCVRAKDALCNSQWSEWSHLRLRRNNKNKHRQNDKQG

Target ORF information:

RefSeq Version	XM_020079566.1
Organism	Paralichthys olivaceus(Japanese flounder)
Definition	Paralichthys olivaceus interleukin 12B (il12b), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_020079566.1

ORF Insert Sequence:

ATGTCTGTAT TTCTACAAGT CTGCAGCCAA AATCCTCTGC GCTATTGGAC TCTGCTCCCC 
AACATTCTGG TTGTGGAGGT GAACGGCTCT CTGGGCCAGC AGCCCCTGAG CTGCTTGGGC 
TCGCCACAGG AGGGGACGAG GAAAGACGAC AAGAGTCAGG ACATTATTTG GAGGAAGAAT 
GGAGTAGTGG AAGAACAGAG GGGAAACTCG TACTTGGTGC AGCTGGAGGA AAGTTTAGGT 
GGGGGCAACT ACACATGCCA CAGAGAGGAC GGATCACTGG TCAATCACAC TGTGGTTCTG 
ATACAGGAGG ACCAAACCGA GAGGAGGAAG ATTCTTGTGA AAACTGATCA AGAGGATTAC 
TTGACGTGCT TGGCTCAAAA TTACAACGGA GAGTTTCACT GCTCTTGGAC CTGGAACAGA 
AATCGTGTTG GCAAAGTAGC ATTGATCCAG GTTGGGCGCA TTTCTAACGA CAGTGACGCC 
CAGTGTTCTG TGGACGTCAG TACCCAGCGC TGGACGTGCT CCTCAGGTCA GAGTAACTTC 
AGCTGCTCAG TCGATGAACA TGGACACAGG ATCTCCTGCC GAGACGAGCA GCACTGCCCC 
TACGCTGAGG AGATCCAGCA GATCTACGTC ACTTTGTACG TGAGGACCGA GCAATTCTTG 
GTGGAGAACT ACTCTAAACA GTTTTACCTG GCAGAGATAG TGAAACCTGA CATGATCTCA 
CAGTTCAGGC GGGGATGCAA AGGGTGTGAC AACCCCTGCA CTGAGTCAAA ACCTGCAAAG 
ACCCTGATGG TCCATTTCAC AGACGTTTGC CAATTTAAAG TCAAGCACAA GGCCAAGGCT 
GTGTGTGTCC GAGCAAAGGA TGCTCTCTGT AACTCCCAGT GGAGCGAATG GAGCCACCTC 
AGATTGAGGA GAAACAATAA GAACAAACAC CGGCAAAATG ATAAACAAGG GTAA