EPHB1 cDNA ORF clone, Panthera pardus(leopard)

Gene
Clones
gRNAs

Summary

Gene Symbol	EPHB1
Entrez Gene ID	109259539
Full Name	EPH receptor B1
Gene Type	protein-coding
Organism	Panthera pardus(leopard)

mRNA and Protein(s)

mRNA	Protein	Name
XM_019437143.1	XP_019292688.1	ephrin type-B receptor 1 isoform X1
XM_019437149.1	XP_019292694.1	ephrin type-B receptor 1 isoform X2

Pathways from BioSystems
Gene Ontology

The following EPHB1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the EPHB1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OPa190463	XM_019437143.1 Latest version!	Panthera pardus EPH receptor B1 (EPHB1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
OPa190464	XM_019437149.1 Latest version!	Panthera pardus EPH receptor B1 (EPHB1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

EPHB1 ( XM_019437143.1 ) cDNA ORF clone, Panthera pardus(leopard) -> XP_019292688.1 Panthera pardus EPH receptor B1 (EPHB1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OPa190463
Clone ID Related Accession (Same CDS sequence)	XM_019437143.1
Accession Version	XM_019437143.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2955bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-12-04
Organism	Panthera pardus(leopard)
Product	ephrin type-B receptor 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_017619850.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Panthera pardus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCCCGG ATTGCCTGCT GCTGCTCCTC CTGGCATCCG CAGTGGCCGC TATGGAAGAA 
ACCTTAATGG ACACCAGAAC GGCTACCGCA GAGCTGGGCT GGACAGCCAA TCCTGCGTCA 
GGGTGGGAAG AAGTCAGTGG CTACGATGAA AACCTGAACA CCATCCGCAC CTACCAGGTG 
TGCAACGTCT TTGAGCCCAA CCAGAACAAC TGGCTGCTCA CCACCTTCAT CAACCGGCGG 
GGGGCTCATC GCATCTACAC AGAGATGCGC TTCACTGTGA GAGACTGCAG CAGCCTCCCC 
AACGTCCCAG GTTCCTGCAA GGAGACCTTC AACTTGTACT ACTATGAGAC GGACTCAGTT 
ATTGCCACCA AGAAGTCAGC CTTCTGGTCT GAGGCCCCCT ACCTCAAAGT AGACACCATT 
GCTGCAGATG AGAGCTTCTC CCAGGTGGAC TTTGGGGGAA GGTTGATGAA GGTCAACACA 
GAAGTCAGGA GCTTTGGCCC TCTTACTCGG AATGGTTTTT ACCTCGCTTT CCAGGATTAT 
GGAGCCTGTA TGTCTCTCCT TTCTGTTCGC GTCTTCTTCA AGAAGTGTCC CAGCATTGTG 
CAAAATTTTG CTGTGTTTCC AGAGACCATG ACTGGGGCAG AGAGCACGTC TCTGGTGATT 
GCTCGGGGGA CATGCATCCC CAATGCAGAG GAAGTGGACG TGCCCATCAA ACTCTACTGC 
AATGGGGATG GAGAGTGGAT GGTGCCCATC GGGCGCTGCA CCTGCAAGCC TGGCTATGAG 
CCCGAGAACA GCGTGGCCTG CAAGGCCTGC CCTGCTGGGA TGTTCAAGGC CAGCCAGGAA 
GCTGAGGGGT GCTCCCACTG CCCCTCCAAC AGCCGATCCC CTTCAGAGGC GTCTCCCATC 
TGTACCTGCC GGACCGGCTA TTACCGAGCG GATTTTGACC CCCCAGAAGT GGCATGTACT 
AGTGTCCCGT CAGGTCCCCG CAATGTCATC TCCATTGTCA ATGAGACATC TATCATTCTG 
GAGTGGCATC CGCCAAGGGA GACAGGCGGG CGGGATGATG TGACCTACAA CATCATCTGT 
AGAAAGTGCC GGGCAGACCG TCGGAGCTGC TCCCGCTGTG ACGACAACGT GGAGTTTGTA 
CCCAGGCAGC TGGGCCTGAC CGAGTGCCGT GTCTCCATCA GCAGCCTGTG GGCCCACACC 
CCCTATACCT TCGACATCCA GGCCGTCAAT GGAGTCTCCA GCAAGAGCCC CTTCCCCCCA 
CAGCATGTCT CCGTCAACAT CACCACGAAC CAAGCCGCCC CTTCCACTGT TCCAATCATG 
CACCAGGTCA GTGCCACCAT GAGGAGCATC ACCTTGTCAT GGCCACAGCC GGAGCAGCCC 
AATGGCATCA TCCTGGACTA TGAGATCCGG TACTATGAGA AGGAGCACAA TGAGTTCAAC 
TCCTCCATGG CCAGGAGCCA GACCAACACG GCGCGGATTG ACGGCCTACG GCCAGGCATG 
GTGTATGTGG TGCAAGTGCG TGCCCGCACG GTGGCGGGCT ACGGCAAGTT CAGTGGCAAG 
ATGTGCTTCC AGACGCTGAC GGACGATGAC TACAAGTCTG AGCTGAGGGA GCAGCTGCCA 
CTGATTGCTG GCTCGGCAGC AGCCGGGGTC GTGTTCGTGG TGTCATTGGT GGCCATCTCC 
ATCGTCTGTA GCAGGAAACG GGCTTACAGC AAAGAGGCCG TGTACAGCGA TAAGCTGCAG 
CATTACAGCA CCGGCCGAGG CTCCCCAGGG ATGAAGATCT ACATTGACCC CTTCACTTAC 
GAGGACCCCA ATGAAGCTGT CCGGGAGTTT GCCAAGGAGA TTGATGTATC TTTTGTGAAA 
ATCGAAGAGG TCATCGGAGC AGGGGAGTTT GGAGAGGTAT ACAAGGGGCG TCTGAAACTT 
CCGGGCAAGA GGGAAATCTA TGTGGCAATC AAGACGTTGA AGGCCGGGTA CTCAGAGAAG 
CAGCGTCGGG ACTTCCTGAG CGAAGCAAGC ATCATGGGCC AGTTTGACCA TCCCAACATC 
ATCCGCCTGG AAGGCGTGGT CACCAAGAGT CGGCCTGTCA TGATCATCAC AGAGTTCATG 
GAGAACGGCG CTTTGGATTC TTTCCTCAGG CAAAATGATG GGCAGTTCAC GGTGATCCAG 
CTGGTAGGGA TGCTCAGGGG CATTGCCGCA GGCATGAAGT ACCTGGCTGA GATGAACTAC 
GTGCATCGGG ACCTGGCTGC TAGGAACATC CTGGTCAACA GTAACCTGGT ATGCAAGGTG 
TCTGACTTTG GCCTTTCCCG CTACCTCCAG GATGACACCT CAGACCCCAC CTACACCAGC 
TCCTTGGGAG GGAAGATCCC CGTGAGATGG ACAGCTCCAG AGGCCATTGC CTACCGCAAG 
TTCACTTCTG CCAGCGATGT CTGGAGCTAC GGGATCGTCA TGTGGGAAGT CATGTCATTT 
GGAGAGAGAC CCTACTGGGA TATGTCCAAC CAAGATGTCA TCAATGCCAT TGAGCAGGAC 
TACCGGCTGC CCCCACCCAT GGACTGTCCA GCTGCTCTAC ACCAGCTCAT GCTGGACTGT 
TGGCAGAAGG ACCGTAACAG CCGGCCCCGC TTTGCGGAGA TTGTCAACAC CCTGGACAAG 
ATGATCCGGA ACCCGGCAAG TCTCAAGACT GTGGCAACCA TCACCGCCGT GCCTTCCCAG 
CCCCTGCTAG ACCGCTCCAT TCCAGACTTC ACGGCCTTTA CCACCGTGGA TGACTGGCTC 
AGTGCCATCA AAATGGTCCA GTACAGGGAC AGCTTCCTCA CTGCCGGCTT CACCTCCCTC 
CAGCTGGTCA CCCAGATGAC ATCAGAAGAC CTCCTGAGAA TAGGGGTCAC CTTGGCAGGT 
CATCAGAAGA AGATCCTGAA CAGCATTCAT TCTATGAGGG TCCAGATGAG TCAGTCACCA 
ACGGCAATGG CATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019292688.1
CDS	907..3861
Translation	MAPDCLLLLLLASAVAAMEETLMDTRTATAELGWTANPASGWEEVSGYDENLNTIRTYQVCNVFEPNQNNWLLTTFINRRGAHRIYTEMRFTVRDCSSLPNVPGSCKETFNLYYYETDSVIATKKSAFWSEAPYLKVDTIAADESFSQVDFGGRLMKVNTEVRSFGPLTRNGFYLAFQDYGACMSLLSVRVFFKKCPSIVQNFAVFPETMTGAESTSLVIARGTCIPNAEEVDVPIKLYCNGDGEWMVPIGRCTCKPGYEPENSVACKACPAGMFKASQEAEGCSHCPSNSRSPSEASPICTCRTGYYRADFDPPEVACTSVPSGPRNVISIVNETSIILEWHPPRETGGRDDVTYNIICRKCRADRRSCSRCDDNVEFVPRQLGLTECRVSISSLWAHTPYTFDIQAVNGVSSKSPFPPQHVSVNITTNQAAPSTVPIMHQVSATMRSITLSWPQPEQPNGIILDYEIRYYEKEHNEFNSSMARSQTNTARIDGLRPGMVYVVQVRARTVAGYGKFSGKMCFQTLTDDDYKSELREQLPLIAGSAAAGVVFVVSLVAISIVCSRKRAYSKEAVYSDKLQHYSTGRGSPGMKIYIDPFTYEDPNEAVREFAKEIDVSFVKIEEVIGAGEFGEVYKGRLKLPGKREIYVAIKTLKAGYSEKQRRDFLSEASIMGQFDHPNIIRLEGVVTKSRPVMIITEFMENGALDSFLRQNDGQFTVIQLVGMLRGIAAGMKYLAEMNYVHRDLAARNILVNSNLVCKVSDFGLSRYLQDDTSDPTYTSSLGGKIPVRWTAPEAIAYRKFTSASDVWSYGIVMWEVMSFGERPYWDMSNQDVINAIEQDYRLPPPMDCPAALHQLMLDCWQKDRNSRPRFAEIVNTLDKMIRNPASLKTVATITAVPSQPLLDRSIPDFTAFTTVDDWLSAIKMVQYRDSFLTAGFTSLQLVTQMTSEDLLRIGVTLAGHQKKILNSIHSMRVQMSQSPTAMA

Target ORF information:

RefSeq Version	XM_019437143.1
Organism	Panthera pardus(leopard)
Definition	Panthera pardus EPH receptor B1 (EPHB1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019437143.1

ORF Insert Sequence:

ATGGCCCCGG ATTGCCTGCT GCTGCTCCTC CTGGCATCCG CAGTGGCCGC TATGGAAGAA 
ACCTTAATGG ACACCAGAAC GGCTACCGCA GAGCTGGGCT GGACAGCCAA TCCTGCGTCA 
GGGTGGGAAG AAGTCAGTGG CTACGATGAA AACCTGAACA CCATCCGCAC CTACCAGGTG 
TGCAACGTCT TTGAGCCCAA CCAGAACAAC TGGCTGCTCA CCACCTTCAT CAACCGGCGG 
GGGGCTCATC GCATCTACAC AGAGATGCGC TTCACTGTGA GAGACTGCAG CAGCCTCCCC 
AACGTCCCAG GTTCCTGCAA GGAGACCTTC AACTTGTACT ACTATGAGAC GGACTCAGTT 
ATTGCCACCA AGAAGTCAGC CTTCTGGTCT GAGGCCCCCT ACCTCAAAGT AGACACCATT 
GCTGCAGATG AGAGCTTCTC CCAGGTGGAC TTTGGGGGAA GGTTGATGAA GGTCAACACA 
GAAGTCAGGA GCTTTGGCCC TCTTACTCGG AATGGTTTTT ACCTCGCTTT CCAGGATTAT 
GGAGCCTGTA TGTCTCTCCT TTCTGTTCGC GTCTTCTTCA AGAAGTGTCC CAGCATTGTG 
CAAAATTTTG CTGTGTTTCC AGAGACCATG ACTGGGGCAG AGAGCACGTC TCTGGTGATT 
GCTCGGGGGA CATGCATCCC CAATGCAGAG GAAGTGGACG TGCCCATCAA ACTCTACTGC 
AATGGGGATG GAGAGTGGAT GGTGCCCATC GGGCGCTGCA CCTGCAAGCC TGGCTATGAG 
CCCGAGAACA GCGTGGCCTG CAAGGCCTGC CCTGCTGGGA TGTTCAAGGC CAGCCAGGAA 
GCTGAGGGGT GCTCCCACTG CCCCTCCAAC AGCCGATCCC CTTCAGAGGC GTCTCCCATC 
TGTACCTGCC GGACCGGCTA TTACCGAGCG GATTTTGACC CCCCAGAAGT GGCATGTACT 
AGTGTCCCGT CAGGTCCCCG CAATGTCATC TCCATTGTCA ATGAGACATC TATCATTCTG 
GAGTGGCATC CGCCAAGGGA GACAGGCGGG CGGGATGATG TGACCTACAA CATCATCTGT 
AGAAAGTGCC GGGCAGACCG TCGGAGCTGC TCCCGCTGTG ACGACAACGT GGAGTTTGTA 
CCCAGGCAGC TGGGCCTGAC CGAGTGCCGT GTCTCCATCA GCAGCCTGTG GGCCCACACC 
CCCTATACCT TCGACATCCA GGCCGTCAAT GGAGTCTCCA GCAAGAGCCC CTTCCCCCCA 
CAGCATGTCT CCGTCAACAT CACCACGAAC CAAGCCGCCC CTTCCACTGT TCCAATCATG 
CACCAGGTCA GTGCCACCAT GAGGAGCATC ACCTTGTCAT GGCCACAGCC GGAGCAGCCC 
AATGGCATCA TCCTGGACTA TGAGATCCGG TACTATGAGA AGGAGCACAA TGAGTTCAAC 
TCCTCCATGG CCAGGAGCCA GACCAACACG GCGCGGATTG ACGGCCTACG GCCAGGCATG 
GTGTATGTGG TGCAAGTGCG TGCCCGCACG GTGGCGGGCT ACGGCAAGTT CAGTGGCAAG 
ATGTGCTTCC AGACGCTGAC GGACGATGAC TACAAGTCTG AGCTGAGGGA GCAGCTGCCA 
CTGATTGCTG GCTCGGCAGC AGCCGGGGTC GTGTTCGTGG TGTCATTGGT GGCCATCTCC 
ATCGTCTGTA GCAGGAAACG GGCTTACAGC AAAGAGGCCG TGTACAGCGA TAAGCTGCAG 
CATTACAGCA CCGGCCGAGG CTCCCCAGGG ATGAAGATCT ACATTGACCC CTTCACTTAC 
GAGGACCCCA ATGAAGCTGT CCGGGAGTTT GCCAAGGAGA TTGATGTATC TTTTGTGAAA 
ATCGAAGAGG TCATCGGAGC AGGGGAGTTT GGAGAGGTAT ACAAGGGGCG TCTGAAACTT 
CCGGGCAAGA GGGAAATCTA TGTGGCAATC AAGACGTTGA AGGCCGGGTA CTCAGAGAAG 
CAGCGTCGGG ACTTCCTGAG CGAAGCAAGC ATCATGGGCC AGTTTGACCA TCCCAACATC 
ATCCGCCTGG AAGGCGTGGT CACCAAGAGT CGGCCTGTCA TGATCATCAC AGAGTTCATG 
GAGAACGGCG CTTTGGATTC TTTCCTCAGG CAAAATGATG GGCAGTTCAC GGTGATCCAG 
CTGGTAGGGA TGCTCAGGGG CATTGCCGCA GGCATGAAGT ACCTGGCTGA GATGAACTAC 
GTGCATCGGG ACCTGGCTGC TAGGAACATC CTGGTCAACA GTAACCTGGT ATGCAAGGTG 
TCTGACTTTG GCCTTTCCCG CTACCTCCAG GATGACACCT CAGACCCCAC CTACACCAGC 
TCCTTGGGAG GGAAGATCCC CGTGAGATGG ACAGCTCCAG AGGCCATTGC CTACCGCAAG 
TTCACTTCTG CCAGCGATGT CTGGAGCTAC GGGATCGTCA TGTGGGAAGT CATGTCATTT 
GGAGAGAGAC CCTACTGGGA TATGTCCAAC CAAGATGTCA TCAATGCCAT TGAGCAGGAC 
TACCGGCTGC CCCCACCCAT GGACTGTCCA GCTGCTCTAC ACCAGCTCAT GCTGGACTGT 
TGGCAGAAGG ACCGTAACAG CCGGCCCCGC TTTGCGGAGA TTGTCAACAC CCTGGACAAG 
ATGATCCGGA ACCCGGCAAG TCTCAAGACT GTGGCAACCA TCACCGCCGT GCCTTCCCAG 
CCCCTGCTAG ACCGCTCCAT TCCAGACTTC ACGGCCTTTA CCACCGTGGA TGACTGGCTC 
AGTGCCATCA AAATGGTCCA GTACAGGGAC AGCTTCCTCA CTGCCGGCTT CACCTCCCTC 
CAGCTGGTCA CCCAGATGAC ATCAGAAGAC CTCCTGAGAA TAGGGGTCAC CTTGGCAGGT 
CATCAGAAGA AGATCCTGAA CAGCATTCAT TCTATGAGGG TCCAGATGAG TCAGTCACCA 
ACGGCAATGG CATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EPHB1 ( XM_019437149.1 ) cDNA ORF clone, Panthera pardus(leopard) -> XP_019292694.1 Panthera pardus EPH receptor B1 (EPHB1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OPa190464
Clone ID Related Accession (Same CDS sequence)	XM_019437149.1
Accession Version	XM_019437149.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2889bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-12-04
Organism	Panthera pardus(leopard)
Product	ephrin type-B receptor 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_017619850.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Panthera pardus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACACCA GAACGGCTAC CGCAGAGCTG GGCTGGACAG CCAATCCTGC GTCAGGGTGG 
GAAGAAGTCA GTGGCTACGA TGAAAACCTG AACACCATCC GCACCTACCA GGTGTGCAAC 
GTCTTTGAGC CCAACCAGAA CAACTGGCTG CTCACCACCT TCATCAACCG GCGGGGGGCT 
CATCGCATCT ACACAGAGAT GCGCTTCACT GTGAGAGACT GCAGCAGCCT CCCCAACGTC 
CCAGGTTCCT GCAAGGAGAC CTTCAACTTG TACTACTATG AGACGGACTC AGTTATTGCC 
ACCAAGAAGT CAGCCTTCTG GTCTGAGGCC CCCTACCTCA AAGTAGACAC CATTGCTGCA 
GATGAGAGCT TCTCCCAGGT GGACTTTGGG GGAAGGTTGA TGAAGGTCAA CACAGAAGTC 
AGGAGCTTTG GCCCTCTTAC TCGGAATGGT TTTTACCTCG CTTTCCAGGA TTATGGAGCC 
TGTATGTCTC TCCTTTCTGT TCGCGTCTTC TTCAAGAAGT GTCCCAGCAT TGTGCAAAAT 
TTTGCTGTGT TTCCAGAGAC CATGACTGGG GCAGAGAGCA CGTCTCTGGT GATTGCTCGG 
GGGACATGCA TCCCCAATGC AGAGGAAGTG GACGTGCCCA TCAAACTCTA CTGCAATGGG 
GATGGAGAGT GGATGGTGCC CATCGGGCGC TGCACCTGCA AGCCTGGCTA TGAGCCCGAG 
AACAGCGTGG CCTGCAAGGC CTGCCCTGCT GGGATGTTCA AGGCCAGCCA GGAAGCTGAG 
GGGTGCTCCC ACTGCCCCTC CAACAGCCGA TCCCCTTCAG AGGCGTCTCC CATCTGTACC 
TGCCGGACCG GCTATTACCG AGCGGATTTT GACCCCCCAG AAGTGGCATG TACTAGTGTC 
CCGTCAGGTC CCCGCAATGT CATCTCCATT GTCAATGAGA CATCTATCAT TCTGGAGTGG 
CATCCGCCAA GGGAGACAGG CGGGCGGGAT GATGTGACCT ACAACATCAT CTGTAGAAAG 
TGCCGGGCAG ACCGTCGGAG CTGCTCCCGC TGTGACGACA ACGTGGAGTT TGTACCCAGG 
CAGCTGGGCC TGACCGAGTG CCGTGTCTCC ATCAGCAGCC TGTGGGCCCA CACCCCCTAT 
ACCTTCGACA TCCAGGCCGT CAATGGAGTC TCCAGCAAGA GCCCCTTCCC CCCACAGCAT 
GTCTCCGTCA ACATCACCAC GAACCAAGCC GCCCCTTCCA CTGTTCCAAT CATGCACCAG 
GTCAGTGCCA CCATGAGGAG CATCACCTTG TCATGGCCAC AGCCGGAGCA GCCCAATGGC 
ATCATCCTGG ACTATGAGAT CCGGTACTAT GAGAAGGAGC ACAATGAGTT CAACTCCTCC 
ATGGCCAGGA GCCAGACCAA CACGGCGCGG ATTGACGGCC TACGGCCAGG CATGGTGTAT 
GTGGTGCAAG TGCGTGCCCG CACGGTGGCG GGCTACGGCA AGTTCAGTGG CAAGATGTGC 
TTCCAGACGC TGACGGACGA TGACTACAAG TCTGAGCTGA GGGAGCAGCT GCCACTGATT 
GCTGGCTCGG CAGCAGCCGG GGTCGTGTTC GTGGTGTCAT TGGTGGCCAT CTCCATCGTC 
TGTAGCAGGA AACGGGCTTA CAGCAAAGAG GCCGTGTACA GCGATAAGCT GCAGCATTAC 
AGCACCGGCC GAGGCTCCCC AGGGATGAAG ATCTACATTG ACCCCTTCAC TTACGAGGAC 
CCCAATGAAG CTGTCCGGGA GTTTGCCAAG GAGATTGATG TATCTTTTGT GAAAATCGAA 
GAGGTCATCG GAGCAGGGGA GTTTGGAGAG GTATACAAGG GGCGTCTGAA ACTTCCGGGC 
AAGAGGGAAA TCTATGTGGC AATCAAGACG TTGAAGGCCG GGTACTCAGA GAAGCAGCGT 
CGGGACTTCC TGAGCGAAGC AAGCATCATG GGCCAGTTTG ACCATCCCAA CATCATCCGC 
CTGGAAGGCG TGGTCACCAA GAGTCGGCCT GTCATGATCA TCACAGAGTT CATGGAGAAC 
GGCGCTTTGG ATTCTTTCCT CAGGCAAAAT GATGGGCAGT TCACGGTGAT CCAGCTGGTA 
GGGATGCTCA GGGGCATTGC CGCAGGCATG AAGTACCTGG CTGAGATGAA CTACGTGCAT 
CGGGACCTGG CTGCTAGGAA CATCCTGGTC AACAGTAACC TGGTATGCAA GGTGTCTGAC 
TTTGGCCTTT CCCGCTACCT CCAGGATGAC ACCTCAGACC CCACCTACAC CAGCTCCTTG 
GGAGGGAAGA TCCCCGTGAG ATGGACAGCT CCAGAGGCCA TTGCCTACCG CAAGTTCACT 
TCTGCCAGCG ATGTCTGGAG CTACGGGATC GTCATGTGGG AAGTCATGTC ATTTGGAGAG 
AGACCCTACT GGGATATGTC CAACCAAGAT GTCATCAATG CCATTGAGCA GGACTACCGG 
CTGCCCCCAC CCATGGACTG TCCAGCTGCT CTACACCAGC TCATGCTGGA CTGTTGGCAG 
AAGGACCGTA ACAGCCGGCC CCGCTTTGCG GAGATTGTCA ACACCCTGGA CAAGATGATC 
CGGAACCCGG CAAGTCTCAA GACTGTGGCA ACCATCACCG CCGTGCCTTC CCAGCCCCTG 
CTAGACCGCT CCATTCCAGA CTTCACGGCC TTTACCACCG TGGATGACTG GCTCAGTGCC 
ATCAAAATGG TCCAGTACAG GGACAGCTTC CTCACTGCCG GCTTCACCTC CCTCCAGCTG 
GTCACCCAGA TGACATCAGA AGACCTCCTG AGAATAGGGG TCACCTTGGC AGGTCATCAG 
AAGAAGATCC TGAACAGCAT TCATTCTATG AGGGTCCAGA TGAGTCAGTC ACCAACGGCA 
ATGGCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019292694.1
CDS	398..3286
Translation	MDTRTATAELGWTANPASGWEEVSGYDENLNTIRTYQVCNVFEPNQNNWLLTTFINRRGAHRIYTEMRFTVRDCSSLPNVPGSCKETFNLYYYETDSVIATKKSAFWSEAPYLKVDTIAADESFSQVDFGGRLMKVNTEVRSFGPLTRNGFYLAFQDYGACMSLLSVRVFFKKCPSIVQNFAVFPETMTGAESTSLVIARGTCIPNAEEVDVPIKLYCNGDGEWMVPIGRCTCKPGYEPENSVACKACPAGMFKASQEAEGCSHCPSNSRSPSEASPICTCRTGYYRADFDPPEVACTSVPSGPRNVISIVNETSIILEWHPPRETGGRDDVTYNIICRKCRADRRSCSRCDDNVEFVPRQLGLTECRVSISSLWAHTPYTFDIQAVNGVSSKSPFPPQHVSVNITTNQAAPSTVPIMHQVSATMRSITLSWPQPEQPNGIILDYEIRYYEKEHNEFNSSMARSQTNTARIDGLRPGMVYVVQVRARTVAGYGKFSGKMCFQTLTDDDYKSELREQLPLIAGSAAAGVVFVVSLVAISIVCSRKRAYSKEAVYSDKLQHYSTGRGSPGMKIYIDPFTYEDPNEAVREFAKEIDVSFVKIEEVIGAGEFGEVYKGRLKLPGKREIYVAIKTLKAGYSEKQRRDFLSEASIMGQFDHPNIIRLEGVVTKSRPVMIITEFMENGALDSFLRQNDGQFTVIQLVGMLRGIAAGMKYLAEMNYVHRDLAARNILVNSNLVCKVSDFGLSRYLQDDTSDPTYTSSLGGKIPVRWTAPEAIAYRKFTSASDVWSYGIVMWEVMSFGERPYWDMSNQDVINAIEQDYRLPPPMDCPAALHQLMLDCWQKDRNSRPRFAEIVNTLDKMIRNPASLKTVATITAVPSQPLLDRSIPDFTAFTTVDDWLSAIKMVQYRDSFLTAGFTSLQLVTQMTSEDLLRIGVTLAGHQKKILNSIHSMRVQMSQSPTAMA

Target ORF information:

RefSeq Version	XM_019437149.1
Organism	Panthera pardus(leopard)
Definition	Panthera pardus EPH receptor B1 (EPHB1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019437149.1

ORF Insert Sequence:

ATGGACACCA GAACGGCTAC CGCAGAGCTG GGCTGGACAG CCAATCCTGC GTCAGGGTGG 
GAAGAAGTCA GTGGCTACGA TGAAAACCTG AACACCATCC GCACCTACCA GGTGTGCAAC 
GTCTTTGAGC CCAACCAGAA CAACTGGCTG CTCACCACCT TCATCAACCG GCGGGGGGCT 
CATCGCATCT ACACAGAGAT GCGCTTCACT GTGAGAGACT GCAGCAGCCT CCCCAACGTC 
CCAGGTTCCT GCAAGGAGAC CTTCAACTTG TACTACTATG AGACGGACTC AGTTATTGCC 
ACCAAGAAGT CAGCCTTCTG GTCTGAGGCC CCCTACCTCA AAGTAGACAC CATTGCTGCA 
GATGAGAGCT TCTCCCAGGT GGACTTTGGG GGAAGGTTGA TGAAGGTCAA CACAGAAGTC 
AGGAGCTTTG GCCCTCTTAC TCGGAATGGT TTTTACCTCG CTTTCCAGGA TTATGGAGCC 
TGTATGTCTC TCCTTTCTGT TCGCGTCTTC TTCAAGAAGT GTCCCAGCAT TGTGCAAAAT 
TTTGCTGTGT TTCCAGAGAC CATGACTGGG GCAGAGAGCA CGTCTCTGGT GATTGCTCGG 
GGGACATGCA TCCCCAATGC AGAGGAAGTG GACGTGCCCA TCAAACTCTA CTGCAATGGG 
GATGGAGAGT GGATGGTGCC CATCGGGCGC TGCACCTGCA AGCCTGGCTA TGAGCCCGAG 
AACAGCGTGG CCTGCAAGGC CTGCCCTGCT GGGATGTTCA AGGCCAGCCA GGAAGCTGAG 
GGGTGCTCCC ACTGCCCCTC CAACAGCCGA TCCCCTTCAG AGGCGTCTCC CATCTGTACC 
TGCCGGACCG GCTATTACCG AGCGGATTTT GACCCCCCAG AAGTGGCATG TACTAGTGTC 
CCGTCAGGTC CCCGCAATGT CATCTCCATT GTCAATGAGA CATCTATCAT TCTGGAGTGG 
CATCCGCCAA GGGAGACAGG CGGGCGGGAT GATGTGACCT ACAACATCAT CTGTAGAAAG 
TGCCGGGCAG ACCGTCGGAG CTGCTCCCGC TGTGACGACA ACGTGGAGTT TGTACCCAGG 
CAGCTGGGCC TGACCGAGTG CCGTGTCTCC ATCAGCAGCC TGTGGGCCCA CACCCCCTAT 
ACCTTCGACA TCCAGGCCGT CAATGGAGTC TCCAGCAAGA GCCCCTTCCC CCCACAGCAT 
GTCTCCGTCA ACATCACCAC GAACCAAGCC GCCCCTTCCA CTGTTCCAAT CATGCACCAG 
GTCAGTGCCA CCATGAGGAG CATCACCTTG TCATGGCCAC AGCCGGAGCA GCCCAATGGC 
ATCATCCTGG ACTATGAGAT CCGGTACTAT GAGAAGGAGC ACAATGAGTT CAACTCCTCC 
ATGGCCAGGA GCCAGACCAA CACGGCGCGG ATTGACGGCC TACGGCCAGG CATGGTGTAT 
GTGGTGCAAG TGCGTGCCCG CACGGTGGCG GGCTACGGCA AGTTCAGTGG CAAGATGTGC 
TTCCAGACGC TGACGGACGA TGACTACAAG TCTGAGCTGA GGGAGCAGCT GCCACTGATT 
GCTGGCTCGG CAGCAGCCGG GGTCGTGTTC GTGGTGTCAT TGGTGGCCAT CTCCATCGTC 
TGTAGCAGGA AACGGGCTTA CAGCAAAGAG GCCGTGTACA GCGATAAGCT GCAGCATTAC 
AGCACCGGCC GAGGCTCCCC AGGGATGAAG ATCTACATTG ACCCCTTCAC TTACGAGGAC 
CCCAATGAAG CTGTCCGGGA GTTTGCCAAG GAGATTGATG TATCTTTTGT GAAAATCGAA 
GAGGTCATCG GAGCAGGGGA GTTTGGAGAG GTATACAAGG GGCGTCTGAA ACTTCCGGGC 
AAGAGGGAAA TCTATGTGGC AATCAAGACG TTGAAGGCCG GGTACTCAGA GAAGCAGCGT 
CGGGACTTCC TGAGCGAAGC AAGCATCATG GGCCAGTTTG ACCATCCCAA CATCATCCGC 
CTGGAAGGCG TGGTCACCAA GAGTCGGCCT GTCATGATCA TCACAGAGTT CATGGAGAAC 
GGCGCTTTGG ATTCTTTCCT CAGGCAAAAT GATGGGCAGT TCACGGTGAT CCAGCTGGTA 
GGGATGCTCA GGGGCATTGC CGCAGGCATG AAGTACCTGG CTGAGATGAA CTACGTGCAT 
CGGGACCTGG CTGCTAGGAA CATCCTGGTC AACAGTAACC TGGTATGCAA GGTGTCTGAC 
TTTGGCCTTT CCCGCTACCT CCAGGATGAC ACCTCAGACC CCACCTACAC CAGCTCCTTG 
GGAGGGAAGA TCCCCGTGAG ATGGACAGCT CCAGAGGCCA TTGCCTACCG CAAGTTCACT 
TCTGCCAGCG ATGTCTGGAG CTACGGGATC GTCATGTGGG AAGTCATGTC ATTTGGAGAG 
AGACCCTACT GGGATATGTC CAACCAAGAT GTCATCAATG CCATTGAGCA GGACTACCGG 
CTGCCCCCAC CCATGGACTG TCCAGCTGCT CTACACCAGC TCATGCTGGA CTGTTGGCAG 
AAGGACCGTA ACAGCCGGCC CCGCTTTGCG GAGATTGTCA ACACCCTGGA CAAGATGATC 
CGGAACCCGG CAAGTCTCAA GACTGTGGCA ACCATCACCG CCGTGCCTTC CCAGCCCCTG 
CTAGACCGCT CCATTCCAGA CTTCACGGCC TTTACCACCG TGGATGACTG GCTCAGTGCC 
ATCAAAATGG TCCAGTACAG GGACAGCTTC CTCACTGCCG GCTTCACCTC CCTCCAGCTG 
GTCACCCAGA TGACATCAGA AGACCTCCTG AGAATAGGGG TCACCTTGGC AGGTCATCAG 
AAGAAGATCC TGAACAGCAT TCATTCTATG AGGGTCCAGA TGAGTCAGTC ACCAACGGCA 
ATGGCATGA