NOS2 cDNA ORF clone, Pan paniscus(pygmy chimpanzee)

Gene
Clones
gRNAs

Summary

Gene Symbol	NOS2
Entrez Gene ID	100976546
Full Name	nitric oxide synthase 2
Gene Type	protein-coding
Organism	Pan paniscus(pygmy chimpanzee)

mRNA and Protein(s)

mRNA	Protein	Name
XM_008965183.1	XP_008963431.1	nitric oxide synthase, inducible
XM_008965183.2	XP_008963431.2	nitric oxide synthase, inducible

Pathways from BioSystems
Gene Ontology

The following NOS2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NOS2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OPa451068	XM_008965183.2 Latest version!	Pan paniscus nitric oxide synthase 2 (NOS2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OPa69000	XM_008965183.1 Latest version!	Pan paniscus nitric oxide synthase 2, inducible (NOS2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

NOS2 ( XM_008965183.2 ) cDNA ORF clone, Pan paniscus(pygmy chimpanzee) -> XP_008963431.2 Pan paniscus nitric oxide synthase 2 (NOS2), mRNA.

Price & Availability↑

CloneID	OPa451068
Clone ID Related Accession (Same CDS sequence)	XM_008965183.2
Accession Version	XM_008965183.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3579bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-04-30
Organism	Pan paniscus(pygmy chimpanzee)
Product	nitric oxide synthase, inducible
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_027885.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 1, 2018 this sequence version replaced XM_008965183.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan paniscus Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 1% of CDS bases ##RefSeq-Attributes-END##

ATGGCCTGTC CTTGGAAATT TCTGTTCAAG ACCAAATTCC ACCAGTATGC AATGAATGGG 
GAAAAAGACA TCAACAACAA TGTGGAGAAA GCCCCCTGTG CCACCTCCAG GAGCCGGGCC 
ATGCGCGAGG GGCTGGGCGC GGCGTGCGAG GCCGCCGGGC CCCGGCTGTG TAGGCTGCTC 
TGCAGGATCC TCCGGCCGAA GCCTGACTGC TGCCCGCGCA GAGCCAGTCC AGTGACACAG 
GATGACCTTC AGTATCACAA CCTCAGCAAG CAGCAGAATG AGTCCCCGCA GCCCCTCGTG 
GAGACGGGAA AGAAGTCTCC AGAATCTCTG GTCAAGCTGG ATGCAACCCC ATTGTCCTCC 
CCACGGCATG TGAGGATCAA AAACTGGGGC AGCGGGATGA CTTTCCAAGA CACACTTCAC 
CATAAAGCCA AAGGGATTTT AACTTGCAGG TCCAAATCTT GCCTGGGGTC CATTATGACT 
CCCAAAAGTT TGACCAGAGG ACCCAGGGAC AAGCCTACCC CTCCAGATGA GCTTCTACCT 
CAAGCTATCG AATTTGTCAA CCAATATTAC GGCTCCTTCA AAGAGGCAAA AATAGAGGAA 
CATCTGGCCA GGGTGGAAGC GGTAACAAAG GAGATAGAAA CAACAGGAAC CTACCAACTG 
ACGGGAGATG AGCTCATCTT CGCCACCAAG CAGGCCTGGC GCAATGCCCC ACGCTGCATT 
GGGAGGATCC AGTGGTCCAA CCTGCAGGTC TTCGATGCCC GCAGCTGTTC CACTGCCCGG 
GAAATGTTTG AACACATCTG CAGACACGTG CGTTACTCCA CCAACAATGG CAACATCAGG 
TCGGCCATCA CCGTGTTCCC CCAGCGGAGT GATGGCAAGC ACGACTTCCG GGTGTGGAAT 
GCTCAGCTCA TCCGCTATGC TGGCTACCAG ATGCCAGATG GCAGCATCAG AGGGGACCCT 
GCCAACGTGG AATTCACTCA GCTGTGCATC GACCTGGGCT GGAAGCCCAA GTACGGCCGC 
TTCGATGTGG TCCCCCTGGT CCTGCAGGCC AATGGCCGTG ACCCTGAGCT CTTCGAAATC 
CCACCTGACC TTGTGCTTGA GGTGGCCATG GAACATCCCA AATACGAGTG GTTTCGGGAA 
CTGGAGCTAA AGTGGTACGC CCTGCCTGCA GTGGCCAACA TGCTGCTTGA GGTGGGCGGC 
CTGGAGTTCC CAGGGTGCCC CTTCAATGGC TGGTACATGG GCACAGAGAT CGGAGTCCGG 
GACTTCTGTG ACGTCCAGCG CTACAACATC CTGGAGGAAG TGGGCAGGAG AATGGGCTTG 
GAAACGCACA AGCTGGCCTC GCTCTGGAAA GACCAGGCTG TCGTTGAGAT CAACATTGCT 
GTGCTCCATA GTTTCCAGAA GCAGAATGTG ACCATCATGG ACCACCACTC GGCTGCAGAA 
TCCTTCATGA AGTACATGCA GAATGAATAC CGGTCCCGTG GGGGCTGCCC GGCAGACTGG 
ATTTGGCTGG TCCCTCCCAT GTCTGGGAGC ATCACCCCCG TGTTTCACCA GGAGATGCTG 
AACTATGTCC TGTCCCCTTT CTACTACTAT CAGGTAGAGG CCTGGAAAAC CCATGTCTGG 
CAGGACGAGA AGCGGAGACC CAAGAGAAGA GAGATTCCAT TGAAAGTCTT GGTCAAAGCT 
GTGCTCTTTG CCTGTATGCT GATGCGCAAG ACAATGGCGT CCCGAGTCAG AGTCACCATC 
CTCTTTGCGA CAGAGACAGG AAAATCAGAG GCGCTGGCCT GGGACCTGGG GGCCTTGTTC 
AGCTGTGCCT TCAACCCCAA GGTTGTCTGC ATGGATAAGT ACAGGCTGAG CTGCCTGGAG 
GAGGAGCGGC TGCTGTTGGT GGTGACCAGT ACGTTTGGCA ATGGAGACTG CCCCGGCAAT 
GGAGAGAAAC TGAAGAAATC GCTCTTCATG CTGAAAGAGC TCAACAACAA ATTCAGGTAC 
GCTGTGTTTG GCCTCGGCTC CAGCATGTAC CCTCGGTTCT GCGCCTTTGC TCATGACATT 
GATCAGAAGC TGTCCCACCT GGGGGCCTCT CAGCTCACCC CGATGGGAGA AGGGGATGAG 
CTCAGTGGGC AGGAGGACGC CTTCCGCAGC TGGGCCGTGC AAACCTTCAA GGCAGCCTGT 
GAGACGTTTG ATGTCCGAGG CAAACAGCAC ATTCAGATCC CCAAGCTCTA CACCTCCAAT 
GTGACCTGGG ACCCGCACCA CTACAGGCTC GTGCAGGACT CACAGCCTTT GGACCTCAGC 
AAAGCCCTCA GCAGCATGCA TGCCAAGAAC GTGTTCACCA TGAGGCTCAA ATCTCGGCAG 
AATCTACAAA GTCCGACATC CAGCCGTGCC ACCATCCTGG TGGAACTCTC CTGTGAGGAT 
GGCCAAGGCC TGAACTACCT GCCGGGGGAG CACCTTGGGG TTTGCCCAGG CAACCAGCCG 
GCCCTGGTCC AAGGCATCCT GGAGCGAGTG GTGGATGGCC CCACACCCCA CCAGACAGTG 
CACCTGGAGG CCCTGGATGA GAGTGGCAGC TACTGGGTCA GTGACAAGAG GCTGCCCCCC 
TGCTCACTCA GCCAGGCCCT CACCTACTTC CTGGACATCA CCACACCCCC AACCCAGCTG 
CTGCTCCAAA AGCTGGCCCA GGTGGCCACA GAAGCGACTG AGAGACAGAG GCTGGAGGCC 
CTTTGCCAGC CCTCAGAGTA CAGCAAGTGG AAGTTCACCA ACAGCCCCAC ATTCCTGGAG 
GTGCTAGAGG AGTTCCCGTC CCTGCGGGTG TCTGCTGGCT TCCTGCTTTC CCAGCTCCCC 
ATTCTGAAGC CCAGGTTCTA CTCCATCAGC TCCTCCCGGG ATCACACGCC CACGGAGATC 
CACCTGACTG TGGCCGTGGT CACCTACCAC ACCCGAGATG GCCAGGGTCC CCTGCACCAC 
GGCGTCTGCA GCACATGGCT CAACAGCCTG AAGCCCCAAG ACCCAGTGCC CTGCTTTGTG 
CGGAATGCCA GCGGCTTCCA CCTCCCCGAG GATCCCTCCC ATCCTTGCAT CCTCATCGGG 
CCTGGCACAG GCATCGCGCC CTTCCGCAGT TTCTGGCAGC AACGGCTCCA TGACTCCCAG 
CACAAGGGAG TGCGGGGAGG CCGCATGACC TTGGTGTTTG GGTGCCGCCG CCCAGATGAG 
GACCACATCT ACCAGGAGGA GATGCTGGAG ATGGCCCAGA AGGGGGTGCT GCATGCGGTG 
CACACAGCCT ATTCCCGCCT GCCTGGCAAG CCCAAGGTCT ATGTTCAGGA CATCCTGCGG 
CAGCAGCTGG CCAGCGAGGT GCTCCGTGTG CTCCACAAGG AGCCAGGCCA CCTCTATGTT 
TGCGGGGATG TGCGCATGGC CCGGGACGTG GCCCATACCC TGAAGCAGCT GGTGGCTGCC 
AAGCTGAAAT TGAATGAGGA GCAGGTCGAG GACTATTTCT TTCAGCTCAA GAGCCAGAAG 
CGCTATCACG AAGATATCTT TGGTGCTGTA TTTCCCTACG AGGCGAAGAA GGACAGGGTG 
GCGGTGCAGC CCAGCAACCT GGAGATGTCA GCGCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008963431.2
CDS	1..3579
Translation	MACPWKFLFKTKFHQYAMNGEKDINNNVEKAPCATSRSRAMREGLGAACEAAGPRLCRLLCRILRPKPDCCPRRASPVTQDDLQYHNLSKQQNESPQPLVETGKKSPESLVKLDATPLSSPRHVRIKNWGSGMTFQDTLHHKAKGILTCRSKSCLGSIMTPKSLTRGPRDKPTPPDELLPQAIEFVNQYYGSFKEAKIEEHLARVEAVTKEIETTGTYQLTGDELIFATKQAWRNAPRCIGRIQWSNLQVFDARSCSTAREMFEHICRHVRYSTNNGNIRSAITVFPQRSDGKHDFRVWNAQLIRYAGYQMPDGSIRGDPANVEFTQLCIDLGWKPKYGRFDVVPLVLQANGRDPELFEIPPDLVLEVAMEHPKYEWFRELELKWYALPAVANMLLEVGGLEFPGCPFNGWYMGTEIGVRDFCDVQRYNILEEVGRRMGLETHKLASLWKDQAVVEINIAVLHSFQKQNVTIMDHHSAAESFMKYMQNEYRSRGGCPADWIWLVPPMSGSITPVFHQEMLNYVLSPFYYYQVEAWKTHVWQDEKRRPKRREIPLKVLVKAVLFACMLMRKTMASRVRVTILFATETGKSEALAWDLGALFSCAFNPKVVCMDKYRLSCLEEERLLLVVTSTFGNGDCPGNGEKLKKSLFMLKELNNKFRYAVFGLGSSMYPRFCAFAHDIDQKLSHLGASQLTPMGEGDELSGQEDAFRSWAVQTFKAACETFDVRGKQHIQIPKLYTSNVTWDPHHYRLVQDSQPLDLSKALSSMHAKNVFTMRLKSRQNLQSPTSSRATILVELSCEDGQGLNYLPGEHLGVCPGNQPALVQGILERVVDGPTPHQTVHLEALDESGSYWVSDKRLPPCSLSQALTYFLDITTPPTQLLLQKLAQVATEATERQRLEALCQPSEYSKWKFTNSPTFLEVLEEFPSLRVSAGFLLSQLPILKPRFYSISSSRDHTPTEIHLTVAVVTYHTRDGQGPLHHGVCSTWLNSLKPQDPVPCFVRNASGFHLPEDPSHPCILIGPGTGIAPFRSFWQQRLHDSQHKGVRGGRMTLVFGCRRPDEDHIYQEEMLEMAQKGVLHAVHTAYSRLPGKPKVYVQDILRQQLASEVLRVLHKEPGHLYVCGDVRMARDVAHTLKQLVAAKLKLNEEQVEDYFFQLKSQKRYHEDIFGAVFPYEAKKDRVAVQPSNLEMSAL

Target ORF information:

RefSeq Version	XM_008965183.2
Organism	Pan paniscus(pygmy chimpanzee)
Definition	Pan paniscus nitric oxide synthase 2 (NOS2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008965183.2

ORF Insert Sequence:

ATGGCCTGTC CTTGGAAATT TCTGTTCAAG ACCAAATTCC ACCAGTATGC AATGAATGGG 
GAAAAAGACA TCAACAACAA TGTGGAGAAA GCCCCCTGTG CCACCTCCAG GAGCCGGGCC 
ATGCGCGAGG GGCTGGGCGC GGCGTGCGAG GCCGCCGGGC CCCGGCTGTG TAGGCTGCTC 
TGCAGGATCC TCCGGCCGAA GCCTGACTGC TGCCCGCGCA GAGCCAGTCC AGTGACACAG 
GATGACCTTC AGTATCACAA CCTCAGCAAG CAGCAGAATG AGTCCCCGCA GCCCCTCGTG 
GAGACGGGAA AGAAGTCTCC AGAATCTCTG GTCAAGCTGG ATGCAACCCC ATTGTCCTCC 
CCACGGCATG TGAGGATCAA AAACTGGGGC AGCGGGATGA CTTTCCAAGA CACACTTCAC 
CATAAAGCCA AAGGGATTTT AACTTGCAGG TCCAAATCTT GCCTGGGGTC CATTATGACT 
CCCAAAAGTT TGACCAGAGG ACCCAGGGAC AAGCCTACCC CTCCAGATGA GCTTCTACCT 
CAAGCTATCG AATTTGTCAA CCAATATTAC GGCTCCTTCA AAGAGGCAAA AATAGAGGAA 
CATCTGGCCA GGGTGGAAGC GGTAACAAAG GAGATAGAAA CAACAGGAAC CTACCAACTG 
ACGGGAGATG AGCTCATCTT CGCCACCAAG CAGGCCTGGC GCAATGCCCC ACGCTGCATT 
GGGAGGATCC AGTGGTCCAA CCTGCAGGTC TTCGATGCCC GCAGCTGTTC CACTGCCCGG 
GAAATGTTTG AACACATCTG CAGACACGTG CGTTACTCCA CCAACAATGG CAACATCAGG 
TCGGCCATCA CCGTGTTCCC CCAGCGGAGT GATGGCAAGC ACGACTTCCG GGTGTGGAAT 
GCTCAGCTCA TCCGCTATGC TGGCTACCAG ATGCCAGATG GCAGCATCAG AGGGGACCCT 
GCCAACGTGG AATTCACTCA GCTGTGCATC GACCTGGGCT GGAAGCCCAA GTACGGCCGC 
TTCGATGTGG TCCCCCTGGT CCTGCAGGCC AATGGCCGTG ACCCTGAGCT CTTCGAAATC 
CCACCTGACC TTGTGCTTGA GGTGGCCATG GAACATCCCA AATACGAGTG GTTTCGGGAA 
CTGGAGCTAA AGTGGTACGC CCTGCCTGCA GTGGCCAACA TGCTGCTTGA GGTGGGCGGC 
CTGGAGTTCC CAGGGTGCCC CTTCAATGGC TGGTACATGG GCACAGAGAT CGGAGTCCGG 
GACTTCTGTG ACGTCCAGCG CTACAACATC CTGGAGGAAG TGGGCAGGAG AATGGGCTTG 
GAAACGCACA AGCTGGCCTC GCTCTGGAAA GACCAGGCTG TCGTTGAGAT CAACATTGCT 
GTGCTCCATA GTTTCCAGAA GCAGAATGTG ACCATCATGG ACCACCACTC GGCTGCAGAA 
TCCTTCATGA AGTACATGCA GAATGAATAC CGGTCCCGTG GGGGCTGCCC GGCAGACTGG 
ATTTGGCTGG TCCCTCCCAT GTCTGGGAGC ATCACCCCCG TGTTTCACCA GGAGATGCTG 
AACTATGTCC TGTCCCCTTT CTACTACTAT CAGGTAGAGG CCTGGAAAAC CCATGTCTGG 
CAGGACGAGA AGCGGAGACC CAAGAGAAGA GAGATTCCAT TGAAAGTCTT GGTCAAAGCT 
GTGCTCTTTG CCTGTATGCT GATGCGCAAG ACAATGGCGT CCCGAGTCAG AGTCACCATC 
CTCTTTGCGA CAGAGACAGG AAAATCAGAG GCGCTGGCCT GGGACCTGGG GGCCTTGTTC 
AGCTGTGCCT TCAACCCCAA GGTTGTCTGC ATGGATAAGT ACAGGCTGAG CTGCCTGGAG 
GAGGAGCGGC TGCTGTTGGT GGTGACCAGT ACGTTTGGCA ATGGAGACTG CCCCGGCAAT 
GGAGAGAAAC TGAAGAAATC GCTCTTCATG CTGAAAGAGC TCAACAACAA ATTCAGGTAC 
GCTGTGTTTG GCCTCGGCTC CAGCATGTAC CCTCGGTTCT GCGCCTTTGC TCATGACATT 
GATCAGAAGC TGTCCCACCT GGGGGCCTCT CAGCTCACCC CGATGGGAGA AGGGGATGAG 
CTCAGTGGGC AGGAGGACGC CTTCCGCAGC TGGGCCGTGC AAACCTTCAA GGCAGCCTGT 
GAGACGTTTG ATGTCCGAGG CAAACAGCAC ATTCAGATCC CCAAGCTCTA CACCTCCAAT 
GTGACCTGGG ACCCGCACCA CTACAGGCTC GTGCAGGACT CACAGCCTTT GGACCTCAGC 
AAAGCCCTCA GCAGCATGCA TGCCAAGAAC GTGTTCACCA TGAGGCTCAA ATCTCGGCAG 
AATCTACAAA GTCCGACATC CAGCCGTGCC ACCATCCTGG TGGAACTCTC CTGTGAGGAT 
GGCCAAGGCC TGAACTACCT GCCGGGGGAG CACCTTGGGG TTTGCCCAGG CAACCAGCCG 
GCCCTGGTCC AAGGCATCCT GGAGCGAGTG GTGGATGGCC CCACACCCCA CCAGACAGTG 
CACCTGGAGG CCCTGGATGA GAGTGGCAGC TACTGGGTCA GTGACAAGAG GCTGCCCCCC 
TGCTCACTCA GCCAGGCCCT CACCTACTTC CTGGACATCA CCACACCCCC AACCCAGCTG 
CTGCTCCAAA AGCTGGCCCA GGTGGCCACA GAAGCGACTG AGAGACAGAG GCTGGAGGCC 
CTTTGCCAGC CCTCAGAGTA CAGCAAGTGG AAGTTCACCA ACAGCCCCAC ATTCCTGGAG 
GTGCTAGAGG AGTTCCCGTC CCTGCGGGTG TCTGCTGGCT TCCTGCTTTC CCAGCTCCCC 
ATTCTGAAGC CCAGGTTCTA CTCCATCAGC TCCTCCCGGG ATCACACGCC CACGGAGATC 
CACCTGACTG TGGCCGTGGT CACCTACCAC ACCCGAGATG GCCAGGGTCC CCTGCACCAC 
GGCGTCTGCA GCACATGGCT CAACAGCCTG AAGCCCCAAG ACCCAGTGCC CTGCTTTGTG 
CGGAATGCCA GCGGCTTCCA CCTCCCCGAG GATCCCTCCC ATCCTTGCAT CCTCATCGGG 
CCTGGCACAG GCATCGCGCC CTTCCGCAGT TTCTGGCAGC AACGGCTCCA TGACTCCCAG 
CACAAGGGAG TGCGGGGAGG CCGCATGACC TTGGTGTTTG GGTGCCGCCG CCCAGATGAG 
GACCACATCT ACCAGGAGGA GATGCTGGAG ATGGCCCAGA AGGGGGTGCT GCATGCGGTG 
CACACAGCCT ATTCCCGCCT GCCTGGCAAG CCCAAGGTCT ATGTTCAGGA CATCCTGCGG 
CAGCAGCTGG CCAGCGAGGT GCTCCGTGTG CTCCACAAGG AGCCAGGCCA CCTCTATGTT 
TGCGGGGATG TGCGCATGGC CCGGGACGTG GCCCATACCC TGAAGCAGCT GGTGGCTGCC 
AAGCTGAAAT TGAATGAGGA GCAGGTCGAG GACTATTTCT TTCAGCTCAA GAGCCAGAAG 
CGCTATCACG AAGATATCTT TGGTGCTGTA TTTCCCTACG AGGCGAAGAA GGACAGGGTG 
GCGGTGCAGC CCAGCAACCT GGAGATGTCA GCGCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NOS2 ( XM_008965183.1 ) cDNA ORF clone, Pan paniscus(pygmy chimpanzee) -> XP_008963431.1 Pan paniscus nitric oxide synthase 2, inducible (NOS2), mRNA.

Price & Availability↑

CloneID	OPa69000
Clone ID Related Accession (Same CDS sequence)	XM_008965183.1
Accession Version	XM_008965183.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3459bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-29
Organism	Pan paniscus(pygmy chimpanzee)
Product	nitric oxide synthase, inducible
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014014308.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pan paniscus Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 1% of CDS bases ##RefSeq-Attributes-END##

ATGCGCGAGG GGCTGGGCGC GGCGTGCGAG GCCGCCGGGC CCCGGCTGTG TAGGCTGCTC 
TGCAGGATCC TCCGGCCGAA GCCTGACTGC TGCCCGCGCA GAGCCAGTCC AGTGACACAG 
GATGACCTTC AGTATCACAA CCTCAGCAAG CAGCAGAATG AGTCCCCGCA GCCCCTCGTG 
GAGACGGGAA AGAAGTCTCC AGAATCTCTG GTCAAGCTGG ATGCAACCCC ATTGTCCTCC 
CCACGGCATG TGAGGATCAA AAACTGGGGC AGCGGGATGA CTTTCCAAGA CACACTTCAC 
CATAAAGCCA AAGGGATTTT AACTTGCAGG TCCAAATCTT GCCTGGGGTC CATTATGACT 
CCCAAAAGTT TGACCAGAGG ACCCAGGGAC AAGCCTACCC CTCCAGATGA GCTTCTACCT 
CAAGCTATCG AATTTGTCAA CCAATATTAC GGCTCCTTCA AAGAGGCAAA AATAGAGGAA 
CATCTGGCCA GGGTGGAAGC GGTAACAAAG GAGATAGAAA CAACAGGAAC CTACCAACTG 
ACGGGAGATG AGCTCATCTT CGCCACCAAG CAGGCCTGGC GCAATGCCCC ACGCTGCATT 
GGGAGGATCC AGTGGTCCAA CCTGCAGGTC TTCGATGCCC GCAGCTGTTC CACTGCCCGG 
GAAATGTTTG AACACATCTG CAGACACGTG CGTTACTCCA CCAACAATGG CAACATCAGG 
TCGGCCATCA CCGTGTTCCC CCAGCGGAGT GATGGCAAGC ACGACTTCCG GGTGTGGAAT 
GCTCAGCTCA TCCGCTATGC TGGCTACCAG ATGCCAGATG GCAGCATCAG AGGGGACCCT 
GCCAACGTGG AATTCACTCA GCTGTGCATC GACCTGGGCT GGAAGCCCAA GTACGGCCGC 
TTCGATGTGG TCCCCCTGGT CCTGCAGGCC AATGGCCGTG ACCCTGAGCT CTTCGAAATC 
CCACCTGACC TTGTGCTTGA GGTGGCCATG GAACATCCCA AATACGAGTG GTTTCGGGAA 
CTGGAGCTAA AGTGGTACGC CCTGCCTGCA GTGGCCAACA TGCTGCTTGA GGTGGGCGGC 
CTGGAGTTCC CAGGGTGCCC CTTCAATGGC TGGTACATGG GCACAGAGAT CGGAGTCCGG 
GACTTCTGTG ACGTCCAGCG CTACAACATC CTGGAGGAAG TGGGCAGGAG AATGGGCTTG 
GAAACGCACA AGCTGGCCTC GCTCTGGAAA GACCAGGCTG TCGTTGAGAT CAACATTGCT 
GTGCTCCATA GTTTCCAGAA GCAGAATGTG ACCATCATGG ACCACCACTC GGCTGCAGAA 
TCCTTCATGA AGTACATGCA GAATGAATAC CGGTCCCGTG GGGGCTGCCC GGCAGACTGG 
ATTTGGCTGG TCCCTCCCAT GTCTGGGAGC ATCACCCCCG TGTTTCACCA GGAGATGCTG 
AACTATGTCC TGTCCCCTTT CTACTACTAT CAGGTAGAGG CCTGGAAAAC CCATGTCTGG 
CAGGACGAGA AGCGGAGACC CAAGAGAAGA GAGATTCCAT TGAAAGTCTT GGTCAAAGCT 
GTGCTCTTTG CCTGTATGCT GATGCGCAAG ACAATGGCGT CCCGAGTCAG AGTCACCATC 
CTCTTTGCGA CAGAGACAGG AAAATCAGAG GCGCTGGCCT GGGACCTGGG GGCCTTGTTC 
AGCTGTGCCT TCAACCCCAA GGTTGTCTGC ATGGATAAGT ACAGGCTGAG CTGCCTGGAG 
GAGGAGCGGC TGCTGTTGGT GGTGACCAGT ACGTTTGGCA ATGGAGACTG CCCCGGCAAT 
GGAGAGAAAC TGAAGAAATC GCTCTTCATG CTGAAAGAGC TCAACAACAA ATTCAGGTAC 
GCTGTGTTTG GCCTCGGCTC CAGCATGTAC CCTCGGTTCT GCGCCTTTGC TCATGACATT 
GATCAGAAGC TGTCCCACCT GGGGGCCTCT CAGCTCACCC CGATGGGAGA AGGGGATGAG 
CTCAGTGGGC AGGAGGACGC CTTCCGCAGC TGGGCCGTGC AAACCTTCAA GGCAGCCTGT 
GAGACGTTTG ATGTCCGAGG CAAACAGCAC ATTCAGATCC CCAAGCTCTA CACCTCCAAT 
GTGACCTGGG ACCCGCACCA CTACAGGCTC GTGCAGGACT CACAGCCTTT GGACCTCAGC 
AAAGCCCTCA GCAGCATGCA TGCCAAGAAC GTGTTCACCA TGAGGCTCAA ATCTCGGCAG 
AATCTACAAA GTCCGACATC CAGCCGTGCC ACCATCCTGG TGGAACTCTC CTGTGAGGAT 
GGCCAAGGCC TGAACTACCT GCCGGGGGAG CACCTTGGGG TTTGCCCAGG CAACCAGCCG 
GCCCTGGTCC AAGGCATCCT GGAGCGAGTG GTGGATGGCC CCACACCCCA CCAGACAGTG 
CACCTGGAGG CCCTGGATGA GAGTGGCAGC TACTGGGTCA GTGACAAGAG GCTGCCCCCC 
TGCTCACTCA GCCAGGCCCT CACCTACTTC CTGGACATCA CCACACCCCC AACCCAGCTG 
CTGCTCCAAA AGCTGGCCCA GGTGGCCACA GAAGCGACTG AGAGACAGAG GCTGGAGGCC 
CTTTGCCAGC CCTCAGAGTA CAGCAAGTGG AAGTTCACCA ACAGCCCCAC ATTCCTGGAG 
GTGCTAGAGG AGTTCCCGTC CCTGCGGGTG TCTGCTGGCT TCCTGCTTTC CCAGCTCCCC 
ATTCTGAAGC CCAGGTTCTA CTCCATCAGC TCCTCCCGGG ATCACACGCC CACGGAGATC 
CACCTGACTG TGGCCGTGGT CACCTACCAC ACCCGAGATG GCCAGGGTCC CCTGCACCAC 
GGCGTCTGCA GCACATGGCT CAACAGCCTG AAGCCCCAAG ACCCAGTGCC CTGCTTTGTG 
CGGAATGCCA GCGGCTTCCA CCTCCCCGAG GATCCCTCCC ATCCTTGCAT CCTCATCGGG 
CCTGGCACAG GCATCGCGCC CTTCCGCAGT TTCTGGCAGC AACGGCTCCA TGACTCCCAG 
CACAAGGGAG TGCGGGGAGG CCGCATGACC TTGGTGTTTG GGTGCCGCCG CCCAGATGAG 
GACCACATCT ACCAGGAGGA GATGCTGGAG ATGGCCCAGA AGGGGGTGCT GCATGCGGTG 
CACACAGCCT ATTCCCGCCT GCCTGGCAAG CCCAAGGTCT ATGTTCAGGA CATCCTGCGG 
CAGCAGCTGG CCAGCGAGGT GCTCCGTGTG CTCCACAAGG AGCCAGGCCA CCTCTATGTT 
TGCGGGGATG TGCGCATGGC CCGGGACGTG GCCCATACCC TGAAGCAGCT GGTGGCTGCC 
AAGCTGAAAT TGAATGAGGA GCAGGTCGAG GACTATTTCT TTCAGCTCAA GAGCCAGAAG 
CGCTATCACG AAGATATCTT TGGTGCTGTA TTTCCCTACG AGGCGAAGAA GGACAGGGTG 
GCGGTGCAGC CCAGCAACCT GGAGATGTCA GCGCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008963431.1
CDS	1..3459
Translation	MREGLGAACEAAGPRLCRLLCRILRPKPDCCPRRASPVTQDDLQYHNLSKQQNESPQPLVETGKKSPESLVKLDATPLSSPRHVRIKNWGSGMTFQDTLHHKAKGILTCRSKSCLGSIMTPKSLTRGPRDKPTPPDELLPQAIEFVNQYYGSFKEAKIEEHLARVEAVTKEIETTGTYQLTGDELIFATKQAWRNAPRCIGRIQWSNLQVFDARSCSTAREMFEHICRHVRYSTNNGNIRSAITVFPQRSDGKHDFRVWNAQLIRYAGYQMPDGSIRGDPANVEFTQLCIDLGWKPKYGRFDVVPLVLQANGRDPELFEIPPDLVLEVAMEHPKYEWFRELELKWYALPAVANMLLEVGGLEFPGCPFNGWYMGTEIGVRDFCDVQRYNILEEVGRRMGLETHKLASLWKDQAVVEINIAVLHSFQKQNVTIMDHHSAAESFMKYMQNEYRSRGGCPADWIWLVPPMSGSITPVFHQEMLNYVLSPFYYYQVEAWKTHVWQDEKRRPKRREIPLKVLVKAVLFACMLMRKTMASRVRVTILFATETGKSEALAWDLGALFSCAFNPKVVCMDKYRLSCLEEERLLLVVTSTFGNGDCPGNGEKLKKSLFMLKELNNKFRYAVFGLGSSMYPRFCAFAHDIDQKLSHLGASQLTPMGEGDELSGQEDAFRSWAVQTFKAACETFDVRGKQHIQIPKLYTSNVTWDPHHYRLVQDSQPLDLSKALSSMHAKNVFTMRLKSRQNLQSPTSSRATILVELSCEDGQGLNYLPGEHLGVCPGNQPALVQGILERVVDGPTPHQTVHLEALDESGSYWVSDKRLPPCSLSQALTYFLDITTPPTQLLLQKLAQVATEATERQRLEALCQPSEYSKWKFTNSPTFLEVLEEFPSLRVSAGFLLSQLPILKPRFYSISSSRDHTPTEIHLTVAVVTYHTRDGQGPLHHGVCSTWLNSLKPQDPVPCFVRNASGFHLPEDPSHPCILIGPGTGIAPFRSFWQQRLHDSQHKGVRGGRMTLVFGCRRPDEDHIYQEEMLEMAQKGVLHAVHTAYSRLPGKPKVYVQDILRQQLASEVLRVLHKEPGHLYVCGDVRMARDVAHTLKQLVAAKLKLNEEQVEDYFFQLKSQKRYHEDIFGAVFPYEAKKDRVAVQPSNLEMSAL

Target ORF information:

RefSeq Version	XM_008965183.1
Organism	Pan paniscus(pygmy chimpanzee)
Definition	Pan paniscus nitric oxide synthase 2, inducible (NOS2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008965183.1

ORF Insert Sequence:

ATGCGCGAGG GGCTGGGCGC GGCGTGCGAG GCCGCCGGGC CCCGGCTGTG TAGGCTGCTC 
TGCAGGATCC TCCGGCCGAA GCCTGACTGC TGCCCGCGCA GAGCCAGTCC AGTGACACAG 
GATGACCTTC AGTATCACAA CCTCAGCAAG CAGCAGAATG AGTCCCCGCA GCCCCTCGTG 
GAGACGGGAA AGAAGTCTCC AGAATCTCTG GTCAAGCTGG ATGCAACCCC ATTGTCCTCC 
CCACGGCATG TGAGGATCAA AAACTGGGGC AGCGGGATGA CTTTCCAAGA CACACTTCAC 
CATAAAGCCA AAGGGATTTT AACTTGCAGG TCCAAATCTT GCCTGGGGTC CATTATGACT 
CCCAAAAGTT TGACCAGAGG ACCCAGGGAC AAGCCTACCC CTCCAGATGA GCTTCTACCT 
CAAGCTATCG AATTTGTCAA CCAATATTAC GGCTCCTTCA AAGAGGCAAA AATAGAGGAA 
CATCTGGCCA GGGTGGAAGC GGTAACAAAG GAGATAGAAA CAACAGGAAC CTACCAACTG 
ACGGGAGATG AGCTCATCTT CGCCACCAAG CAGGCCTGGC GCAATGCCCC ACGCTGCATT 
GGGAGGATCC AGTGGTCCAA CCTGCAGGTC TTCGATGCCC GCAGCTGTTC CACTGCCCGG 
GAAATGTTTG AACACATCTG CAGACACGTG CGTTACTCCA CCAACAATGG CAACATCAGG 
TCGGCCATCA CCGTGTTCCC CCAGCGGAGT GATGGCAAGC ACGACTTCCG GGTGTGGAAT 
GCTCAGCTCA TCCGCTATGC TGGCTACCAG ATGCCAGATG GCAGCATCAG AGGGGACCCT 
GCCAACGTGG AATTCACTCA GCTGTGCATC GACCTGGGCT GGAAGCCCAA GTACGGCCGC 
TTCGATGTGG TCCCCCTGGT CCTGCAGGCC AATGGCCGTG ACCCTGAGCT CTTCGAAATC 
CCACCTGACC TTGTGCTTGA GGTGGCCATG GAACATCCCA AATACGAGTG GTTTCGGGAA 
CTGGAGCTAA AGTGGTACGC CCTGCCTGCA GTGGCCAACA TGCTGCTTGA GGTGGGCGGC 
CTGGAGTTCC CAGGGTGCCC CTTCAATGGC TGGTACATGG GCACAGAGAT CGGAGTCCGG 
GACTTCTGTG ACGTCCAGCG CTACAACATC CTGGAGGAAG TGGGCAGGAG AATGGGCTTG 
GAAACGCACA AGCTGGCCTC GCTCTGGAAA GACCAGGCTG TCGTTGAGAT CAACATTGCT 
GTGCTCCATA GTTTCCAGAA GCAGAATGTG ACCATCATGG ACCACCACTC GGCTGCAGAA 
TCCTTCATGA AGTACATGCA GAATGAATAC CGGTCCCGTG GGGGCTGCCC GGCAGACTGG 
ATTTGGCTGG TCCCTCCCAT GTCTGGGAGC ATCACCCCCG TGTTTCACCA GGAGATGCTG 
AACTATGTCC TGTCCCCTTT CTACTACTAT CAGGTAGAGG CCTGGAAAAC CCATGTCTGG 
CAGGACGAGA AGCGGAGACC CAAGAGAAGA GAGATTCCAT TGAAAGTCTT GGTCAAAGCT 
GTGCTCTTTG CCTGTATGCT GATGCGCAAG ACAATGGCGT CCCGAGTCAG AGTCACCATC 
CTCTTTGCGA CAGAGACAGG AAAATCAGAG GCGCTGGCCT GGGACCTGGG GGCCTTGTTC 
AGCTGTGCCT TCAACCCCAA GGTTGTCTGC ATGGATAAGT ACAGGCTGAG CTGCCTGGAG 
GAGGAGCGGC TGCTGTTGGT GGTGACCAGT ACGTTTGGCA ATGGAGACTG CCCCGGCAAT 
GGAGAGAAAC TGAAGAAATC GCTCTTCATG CTGAAAGAGC TCAACAACAA ATTCAGGTAC 
GCTGTGTTTG GCCTCGGCTC CAGCATGTAC CCTCGGTTCT GCGCCTTTGC TCATGACATT 
GATCAGAAGC TGTCCCACCT GGGGGCCTCT CAGCTCACCC CGATGGGAGA AGGGGATGAG 
CTCAGTGGGC AGGAGGACGC CTTCCGCAGC TGGGCCGTGC AAACCTTCAA GGCAGCCTGT 
GAGACGTTTG ATGTCCGAGG CAAACAGCAC ATTCAGATCC CCAAGCTCTA CACCTCCAAT 
GTGACCTGGG ACCCGCACCA CTACAGGCTC GTGCAGGACT CACAGCCTTT GGACCTCAGC 
AAAGCCCTCA GCAGCATGCA TGCCAAGAAC GTGTTCACCA TGAGGCTCAA ATCTCGGCAG 
AATCTACAAA GTCCGACATC CAGCCGTGCC ACCATCCTGG TGGAACTCTC CTGTGAGGAT 
GGCCAAGGCC TGAACTACCT GCCGGGGGAG CACCTTGGGG TTTGCCCAGG CAACCAGCCG 
GCCCTGGTCC AAGGCATCCT GGAGCGAGTG GTGGATGGCC CCACACCCCA CCAGACAGTG 
CACCTGGAGG CCCTGGATGA GAGTGGCAGC TACTGGGTCA GTGACAAGAG GCTGCCCCCC 
TGCTCACTCA GCCAGGCCCT CACCTACTTC CTGGACATCA CCACACCCCC AACCCAGCTG 
CTGCTCCAAA AGCTGGCCCA GGTGGCCACA GAAGCGACTG AGAGACAGAG GCTGGAGGCC 
CTTTGCCAGC CCTCAGAGTA CAGCAAGTGG AAGTTCACCA ACAGCCCCAC ATTCCTGGAG 
GTGCTAGAGG AGTTCCCGTC CCTGCGGGTG TCTGCTGGCT TCCTGCTTTC CCAGCTCCCC 
ATTCTGAAGC CCAGGTTCTA CTCCATCAGC TCCTCCCGGG ATCACACGCC CACGGAGATC 
CACCTGACTG TGGCCGTGGT CACCTACCAC ACCCGAGATG GCCAGGGTCC CCTGCACCAC 
GGCGTCTGCA GCACATGGCT CAACAGCCTG AAGCCCCAAG ACCCAGTGCC CTGCTTTGTG 
CGGAATGCCA GCGGCTTCCA CCTCCCCGAG GATCCCTCCC ATCCTTGCAT CCTCATCGGG 
CCTGGCACAG GCATCGCGCC CTTCCGCAGT TTCTGGCAGC AACGGCTCCA TGACTCCCAG 
CACAAGGGAG TGCGGGGAGG CCGCATGACC TTGGTGTTTG GGTGCCGCCG CCCAGATGAG 
GACCACATCT ACCAGGAGGA GATGCTGGAG ATGGCCCAGA AGGGGGTGCT GCATGCGGTG 
CACACAGCCT ATTCCCGCCT GCCTGGCAAG CCCAAGGTCT ATGTTCAGGA CATCCTGCGG 
CAGCAGCTGG CCAGCGAGGT GCTCCGTGTG CTCCACAAGG AGCCAGGCCA CCTCTATGTT 
TGCGGGGATG TGCGCATGGC CCGGGACGTG GCCCATACCC TGAAGCAGCT GGTGGCTGCC 
AAGCTGAAAT TGAATGAGGA GCAGGTCGAG GACTATTTCT TTCAGCTCAA GAGCCAGAAG 
CGCTATCACG AAGATATCTT TGGTGCTGTA TTTCCCTACG AGGCGAAGAA GGACAGGGTG 
GCGGTGCAGC CCAGCAACCT GGAGATGTCA GCGCTCTGA