| Gene Symbol | SHMT1 |
| Entrez Gene ID | 443544 |
| Full Name | serine hydroxymethyltransferase 1 |
| Synonyms | SHMT,SHMT2 |
| Gene Type | protein-coding |
| Organism | Ovis aries(sheep) |
ORF » Species Summary » Ovis aries » SHMT1 cDNA ORF clone
| Gene Symbol | SHMT1 |
| Entrez Gene ID | 443544 |
| Full Name | serine hydroxymethyltransferase 1 |
| Synonyms | SHMT,SHMT2 |
| Gene Type | protein-coding |
| Organism | Ovis aries(sheep) |
| mRNA | Protein | Name |
|---|---|---|
| XM_012185115.1 | XP_012040505.1 | serine hydroxymethyltransferase, cytosolic isoform X1 |
| NM_001009469.1 | NP_001009469.1 | serine hydroxymethyltransferase, cytosolic |
| XM_027973955.1 | XP_027829756.1 | serine hydroxymethyltransferase, cytosolic isoform X1 |
| XM_027973956.1 | XP_027829757.1 | serine hydroxymethyltransferase, cytosolic isoform X1 |
| XM_027973957.1 | XP_027829758.1 | serine hydroxymethyltransferase, cytosolic isoform X1 |
Low serine hydroxymethyltransferase activity in the human placenta has important implications for fetal glycine supply.
Lewis RM, Godfrey KM, Jackson AA, Cameron IT, Hanson MA
The Journal of clinical endocrinology and metabolism90(3)1594-8(2005 Mar)
Disruption of distal interactions of Arg 262 and of substrate binding to Ser 52 affect catalysis of sheep liver cytosolic serine hydroxymethyltransferase.
Jala VR, Ambili M, Prakash V, Rao NA, Savithri HS
Indian journal of biochemistry & biophysics40(4)226-37(2003 Aug)
cDNA cloning, overexpression in Escherichia coli, purification and characterization of sheep liver cytosolic serine hydroxymethyltransferase.
Jagath-Reddy J, Ganesan K, Savithri HS, Datta A, Rao NA
European journal of biochemistry230(2)533-7(1995 Jun)
The primary structure of sheep liver cytosolic serine hydroxymethyltransferase and an analysis of the evolutionary relationships among serine hydroxymethyltransferases.
Usha R, Savithri HS, Rao NA
Biochimica et biophysica acta1204(1)75-83(1994 Jan)
GeneRIFs: Gene References Into Functions What's a GeneRIF?
The low activity of SHMT in the human and rat placenta suggests that, unlike in the sheep, placental conversion of serine to glycine is not a major source of fetal glycine in these species.
Title: Low serine hydroxymethyltransferase activity in the human placenta has important implications for fetal glycine supply.
characterization of sheep liver cytosolic serine hydroxymethyltransferase
Title: Disruption of distal interactions of Arg 262 and of substrate binding to Ser 52 affect catalysis of sheep liver cytosolic serine hydroxymethyltransferase.
The following SHMT1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SHMT1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.
| CloneID | OSm00062 | |
| Clone ID Related Accession (Same CDS sequence) | NM_001009469.1 | |
| Accession Version | NM_001009469.1 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 1455bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2019-12-24 | |
| Organism | Ovis aries(sheep) | |
| Product | serine hydroxymethyltransferase, cytosolic | |
| Comment | Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from X80024.1. ##Evidence-Data-START## Transcript exon combination :: X80024.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA104494976, SAMEA104494977 [ECO:0000348] ##Evidence-Data-END## | |
1 | ATGGCAGCTC CAGTCAACAA GGCACCCAGA GATGCCGATT TGTGGTCCTT GCATGAGAAG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | NP_001009469.1 |
| CDS | 27..1481 |
| Translation |
Target ORF information:
Target ORF information:
|
NM_001009469.1 |
1 | ATGGCAGCTC CAGTCAACAA GGCACCCAGA GATGCCGATT TGTGGTCCTT GCATGAGAAG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | OSm21920 | |
| Clone ID Related Accession (Same CDS sequence) | XM_012185115.1 | |
| Accession Version | XM_012185115.1 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 1464bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2015-12-16 | |
| Organism | Ovis aries(sheep) | |
| Product | serine hydroxymethyltransferase, cytosolic isoform X1 | |
| Comment | Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014639020.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## | |
1 | ATGGCAGCTC CAGTCAACAA GGCACCCAGA GATGCCGATT TGTGGTCCTT GCATGAGAAG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | XP_012040505.1 |
| CDS | 84..1547 |
| Translation |
Target ORF information:
Target ORF information:
|
XM_012185115.1 |
1 | ATGGCAGCTC CAGTCAACAA GGCACCCAGA GATGCCGATT TGTGGTCCTT GCATGAGAAG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | OSm59787 | |
| Clone ID Related Accession (Same CDS sequence) | XM_027973955.1 , XM_027973956.1 , XM_027973957.1 | |
| Accession Version | XM_027973955.1 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 1455bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2019-02-05 | |
| Organism | Ovis aries(sheep) | |
| Product | serine hydroxymethyltransferase, cytosolic isoform X1 | |
| Comment | Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_040262.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Ovis aries Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## | |
1 | ATGGCAGCTC CAGTCAACAA GGCACCCAGA GATGCCGATT TGTGGTCCTT GCATGAGAAG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | XP_027829756.1 |
| CDS | 141..1595 |
| Translation |
Target ORF information:
Target ORF information:
|
XM_027973955.1 |
1 | ATGGCAGCTC CAGTCAACAA GGCACCCAGA GATGCCGATT TGTGGTCCTT GCATGAGAAG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | OSm59787 | |
| Clone ID Related Accession (Same CDS sequence) | XM_027973955.1 , XM_027973956.1 , XM_027973957.1 | |
| Accession Version | XM_027973956.1 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 1455bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2019-02-05 | |
| Organism | Ovis aries(sheep) | |
| Product | serine hydroxymethyltransferase, cytosolic isoform X1 | |
| Comment | Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_040262.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Ovis aries Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## | |
1 | ATGGCAGCTC CAGTCAACAA GGCACCCAGA GATGCCGATT TGTGGTCCTT GCATGAGAAG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | XP_027829757.1 |
| CDS | 226..1680 |
| Translation |
Target ORF information:
Target ORF information:
|
XM_027973956.1 |
1 | ATGGCAGCTC CAGTCAACAA GGCACCCAGA GATGCCGATT TGTGGTCCTT GCATGAGAAG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | OSm59787 | |
| Clone ID Related Accession (Same CDS sequence) | XM_027973955.1 , XM_027973956.1 , XM_027973957.1 | |
| Accession Version | XM_027973957.1 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 1455bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2019-02-05 | |
| Organism | Ovis aries(sheep) | |
| Product | serine hydroxymethyltransferase, cytosolic isoform X1 | |
| Comment | Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_040262.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Ovis aries Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## | |
1 | ATGGCAGCTC CAGTCAACAA GGCACCCAGA GATGCCGATT TGTGGTCCTT GCATGAGAAG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | XP_027829758.1 |
| CDS | 67..1521 |
| Translation |
Target ORF information:
Target ORF information:
|
XM_027973957.1 |
1 | ATGGCAGCTC CAGTCAACAA GGCACCCAGA GATGCCGATT TGTGGTCCTT GCATGAGAAG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
Low serine hydroxymethyltransferase activity in the human placenta has important implications for fetal glycine supply. |
Disruption of distal interactions of Arg 262 and of substrate binding to Ser 52 affect catalysis of sheep liver cytosolic serine hydroxymethyltransferase. |
cDNA cloning, overexpression in Escherichia coli, purification and characterization of sheep liver cytosolic serine hydroxymethyltransferase. |
The primary structure of sheep liver cytosolic serine hydroxymethyltransferase and an analysis of the evolutionary relationships among serine hydroxymethyltransferases. |