LOC115825422 cDNA ORF clone,

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gRNAs

The following LOC115825422 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC115825422 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMi208193	XM_030789254.1 Latest version!	Chanos chanos TGF-beta receptor type-2-like (LOC115825422), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC115825422 ( XM_030789254.1 ) cDNA ORF clone, -> XP_030645114.1 Chanos chanos TGF-beta receptor type-2-like (LOC115825422), mRNA.

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CloneID	OMi208193
Clone ID Related Accession (Same CDS sequence)	XM_030789254.1
Accession Version	XM_030789254.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1863bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-15
Organism	Chanos chanos(milkfish)
Product	TGF-beta receptor type-2-like
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_044506.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Chanos chanos Annotation Release 100 Annotation Version :: 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 13% of CDS bases ##RefSeq-Attributes-END##

ATGGCCTCTT ACTGTGGGAA TTCAGGGGAG GTGTGTGTGA GCGTCTGGGA GAAGTCAGAG 
GGTAACACCA TTGACACTTT CTGCCATAAC CCAGAGAATC CCATTCACGG CATTATGCTG 
AAGGACTACA ATAACAGCAG GTGTGAGATG GGAAGAGAGG GAAGGAATGG AGAGATTGAC 
ATCTGCTCCT GCTCAGAGGA CGAGTGTAAC GACATAGTCT ACTTCAACAC ACCCTCCCTG 
TCTAAGCTAT CTGCTAACAC TGGTTTCAAA TTACGCAAAC TCTGCAAATT CTGCGATGAA 
CAGCCTTCCA CCTGTAACAG CACCTCCTCC TGTCTCTCAA ACTGCTCCAT CACCGCCATC 
TGTGAAGACC CCTCTGAAAT CTGCGTTAGT ATCTGGAGGG AGAAGTCAGA AGGTAACACC 
ATTGACACTT TCTGCCATAA CCCAGAGAAT CCCATTCACG GCATTATGCT GAAGGACTAC 
AATAACAGCA GGTGTGAGAT GAGAAGAAGA GAGGGAACGA ACGGAGAGGT TGACATCTGC 
TCCTGCTCAG AGGACGAGTG TAACGACATA GTCTACTTCA CCACACCCTG CTCTCCCCGC 
GTGGAAACGA GCCTGGCTGT CTCCCTGGCC GTCGTCTGTT TGGTTCCTCT GCTGGTCTTC 
GCCGTGGTGA TCGTCTCACT GTTCTACTTT TATCAAGTCT GTTGCCAGCA TAAGAAAGGA 
GGCGTCCACG ACCGCAGCGA AATCCGCGCC ATCATCACGG AGGACGACCG GTCGGACAGC 
AGCTCCACCC ACGCCAACAG TCTGAATCAC AACACAGAAC TGCTGCCCAT CGAACTGGAC 
ATGCAGGTGG GGAAAGGTCG CTTCGCGGAG GTCTACAAGG CCAAGCTCAA ACAGGGTTCC 
TCCGCAGCCG GGGAGCAGTT CCAAACCGTT GCTGTCAAAG TTTTCCCTTA CGAAGAGCAC 
CTGTCCTGGA AGAATGAGAA GGACATCTTC TCTGACGCTG AGCTTCGTCA TGAGAACATC 
CTCCACTTCC TCACCGCCGA GGAGAGAAAA TCTCCCGTCT CCAAACAGTA CTGGCTGATA 
ACGGCCTATC ACGAGCAAGG TAACCTGCAG GACTACCTGT CTCGTCACGT CGTCTCCTGG 
GCCGAACTGT GCGTGCTCGG AGGGTCGCTG GCGCGAGGCG TGGCTCATCT CCATAGCGAC 
CGCACGCCCT GCGGCGGACA GAAGGTGCCT ATCGCTCACC GGGACCTGAA GAGCTCCAAC 
ATCCTGGTGA AGCATGACCT CAGCTGCTGT CTGTGTGACT TTGGGCTGAG CCTGCGGTTG 
GACAGCTCTC TGTCCGTGGA CGAGTTAGCC AACAGCGGTC AGGTGGGGAC AGCCAGGTAT 
ATGGCGCCTG AGGTCCTGGA GTCCAGGATA AACCTGGAGA ACATCGAGTC CTTCAAACAG 
ACGGATGTCT ACTCCATGGC TCTGGTACTG TGGGAGATGA CGTCGAGGTG TGATGCCATT 
GGAGATGTGA AGGAGTATGA GCCTCCCTTC CATAAAGTGC AGGAACATCC GTGTGTGGAG 
AGTATGAAGG ACAGTGTCAT CAGAGACAGA GAACGACCAG ACATCCCCAA CAGCTGGGCT 
AACCACCTGG GTGTTCAGTC TGTCTGTAAG ACCATAGAAG AGTGTTGGGA CCATGATCCT 
GAAGCTCGTC TGACGGCGCT GTGTGTGGCT GAGCGCTTCA TCCAGCTCGA ACAGCTGGAC 
AAACTCTCCC AGCGCAGCTC CTCACAGGAG AAAATCCCAC AGACCTCTGT GAGCGACGAA 
AAAAAGAGCA CCACAGTGCC CTCTGCTGGG CTGACAGAGA AACAACACCA CCACCAGCAC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030645114.1
CDS	1..1863
Translation	MASYCGNSGEVCVSVWEKSEGNTIDTFCHNPENPIHGIMLKDYNNSRCEMGREGRNGEIDICSCSEDECNDIVYFNTPSLSKLSANTGFKLRKLCKFCDEQPSTCNSTSSCLSNCSITAICEDPSEICVSIWREKSEGNTIDTFCHNPENPIHGIMLKDYNNSRCEMRRREGTNGEVDICSCSEDECNDIVYFTTPCSPRVETSLAVSLAVVCLVPLLVFAVVIVSLFYFYQVCCQHKKGGVHDRSEIRAIITEDDRSDSSSTHANSLNHNTELLPIELDMQVGKGRFAEVYKAKLKQGSSAAGEQFQTVAVKVFPYEEHLSWKNEKDIFSDAELRHENILHFLTAEERKSPVSKQYWLITAYHEQGNLQDYLSRHVVSWAELCVLGGSLARGVAHLHSDRTPCGGQKVPIAHRDLKSSNILVKHDLSCCLCDFGLSLRLDSSLSVDELANSGQVGTARYMAPEVLESRINLENIESFKQTDVYSMALVLWEMTSRCDAIGDVKEYEPPFHKVQEHPCVESMKDSVIRDRERPDIPNSWANHLGVQSVCKTIEECWDHDPEARLTALCVAERFIQLEQLDKLSQRSSSQEKIPQTSVSDEKKSTTVPSAGLTEKQHHHQH

Target ORF information:

RefSeq Version	XM_030789254.1
Organism	Chanos chanos(milkfish)
Definition	Chanos chanos TGF-beta receptor type-2-like (LOC115825422), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030789254.1

ORF Insert Sequence:

ATGGCCTCTT ACTGTGGGAA TTCAGGGGAG GTGTGTGTGA GCGTCTGGGA GAAGTCAGAG 
GGTAACACCA TTGACACTTT CTGCCATAAC CCAGAGAATC CCATTCACGG CATTATGCTG 
AAGGACTACA ATAACAGCAG GTGTGAGATG GGAAGAGAGG GAAGGAATGG AGAGATTGAC 
ATCTGCTCCT GCTCAGAGGA CGAGTGTAAC GACATAGTCT ACTTCAACAC ACCCTCCCTG 
TCTAAGCTAT CTGCTAACAC TGGTTTCAAA TTACGCAAAC TCTGCAAATT CTGCGATGAA 
CAGCCTTCCA CCTGTAACAG CACCTCCTCC TGTCTCTCAA ACTGCTCCAT CACCGCCATC 
TGTGAAGACC CCTCTGAAAT CTGCGTTAGT ATCTGGAGGG AGAAGTCAGA AGGTAACACC 
ATTGACACTT TCTGCCATAA CCCAGAGAAT CCCATTCACG GCATTATGCT GAAGGACTAC 
AATAACAGCA GGTGTGAGAT GAGAAGAAGA GAGGGAACGA ACGGAGAGGT TGACATCTGC 
TCCTGCTCAG AGGACGAGTG TAACGACATA GTCTACTTCA CCACACCCTG CTCTCCCCGC 
GTGGAAACGA GCCTGGCTGT CTCCCTGGCC GTCGTCTGTT TGGTTCCTCT GCTGGTCTTC 
GCCGTGGTGA TCGTCTCACT GTTCTACTTT TATCAAGTCT GTTGCCAGCA TAAGAAAGGA 
GGCGTCCACG ACCGCAGCGA AATCCGCGCC ATCATCACGG AGGACGACCG GTCGGACAGC 
AGCTCCACCC ACGCCAACAG TCTGAATCAC AACACAGAAC TGCTGCCCAT CGAACTGGAC 
ATGCAGGTGG GGAAAGGTCG CTTCGCGGAG GTCTACAAGG CCAAGCTCAA ACAGGGTTCC 
TCCGCAGCCG GGGAGCAGTT CCAAACCGTT GCTGTCAAAG TTTTCCCTTA CGAAGAGCAC 
CTGTCCTGGA AGAATGAGAA GGACATCTTC TCTGACGCTG AGCTTCGTCA TGAGAACATC 
CTCCACTTCC TCACCGCCGA GGAGAGAAAA TCTCCCGTCT CCAAACAGTA CTGGCTGATA 
ACGGCCTATC ACGAGCAAGG TAACCTGCAG GACTACCTGT CTCGTCACGT CGTCTCCTGG 
GCCGAACTGT GCGTGCTCGG AGGGTCGCTG GCGCGAGGCG TGGCTCATCT CCATAGCGAC 
CGCACGCCCT GCGGCGGACA GAAGGTGCCT ATCGCTCACC GGGACCTGAA GAGCTCCAAC 
ATCCTGGTGA AGCATGACCT CAGCTGCTGT CTGTGTGACT TTGGGCTGAG CCTGCGGTTG 
GACAGCTCTC TGTCCGTGGA CGAGTTAGCC AACAGCGGTC AGGTGGGGAC AGCCAGGTAT 
ATGGCGCCTG AGGTCCTGGA GTCCAGGATA AACCTGGAGA ACATCGAGTC CTTCAAACAG 
ACGGATGTCT ACTCCATGGC TCTGGTACTG TGGGAGATGA CGTCGAGGTG TGATGCCATT 
GGAGATGTGA AGGAGTATGA GCCTCCCTTC CATAAAGTGC AGGAACATCC GTGTGTGGAG 
AGTATGAAGG ACAGTGTCAT CAGAGACAGA GAACGACCAG ACATCCCCAA CAGCTGGGCT 
AACCACCTGG GTGTTCAGTC TGTCTGTAAG ACCATAGAAG AGTGTTGGGA CCATGATCCT 
GAAGCTCGTC TGACGGCGCT GTGTGTGGCT GAGCGCTTCA TCCAGCTCGA ACAGCTGGAC 
AAACTCTCCC AGCGCAGCTC CTCACAGGAG AAAATCCCAC AGACCTCTGT GAGCGACGAA 
AAAAAGAGCA CCACAGTGCC CTCTGCTGGG CTGACAGAGA AACAACACCA CCACCAGCAC 
TAA