crim1 cDNA ORF clone,

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gRNAs

The following crim1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the crim1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OIn93268	XM_028395915.1 Latest version!	Parambassis ranga cysteine rich transmembrane BMP regulator 1 (crim1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

crim1 ( XM_028395915.1 ) cDNA ORF clone, -> XP_028251716.1 Parambassis ranga cysteine rich transmembrane BMP regulator 1 (crim1), mRNA.

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CloneID	OIn93268
Clone ID Related Accession (Same CDS sequence)	XM_028395915.1
Accession Version	XM_028395915.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2805bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-03-13
Organism	Parambassis ranga(Indian glassy fish)
Product	cysteine-rich motor neuron 1 protein
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_041042.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Parambassis ranga Annotation Release 100 Annotation Version :: 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCAGCA AATGCTCTTT TGTGTTATTG AATGTCCTCT TTCTGGTGCG GGCGCTCTCG 
GCTCATGAGT GCACGCCGTG TAACCTCCGG ACTTGTCCCG TCAAACCCCA GCATGGTTGC 
GAGGGTGGCC GTACCTTGGC CAGGGATCCA TGCGGATGCT GTGATCACTG CACCCGGCGT 
GAGTGGGAGC CCTGCGGCGG TCAGGATTGG ACGCACGGCT ACTGCGCCCT GGGACTGACC 
TGCGCCTCCC TCAACAAAAC TGGAGCTGCT ACCATCCCTG AAATTGGAGT GTGTAAAGAA 
CTTCCTGACC ATCCAGATGC AGGTCTCGAG GATGAGCGCT GTCCCCTGAT GACGGGCTGT 
GACAGAGCAG GTGGCCAGTG TGTGTGTGAT GCACGTCACT CTTGCTTGGG CTCTTTCACA 
TACTTGGACC AGGAAACCTG CATGAAGGCC AGCAAGTCAG ATGGCCGGAG GCACGAGCAC 
CGGGAAAGGC ACAGAGAGAA GTACGTGGGA CCATCTAACC CAGCGTGTAT GTTCTCTGGG 
TGTAACTTGA CCGCCGAGGG TTGTGTGTGT GAGTCTCAGA GCTGCCATCA CCACTTCGCC 
TACATCAACC GCAGCCAGTG CCAGGAAGCC GCAGAGGAGC TGAGGTGTGC TGGTGTGACG 
TGTCCTGCTC TGCAGGTGCC CTCCTCCTGC CCTGAGGGCT CAGTCCTGAC CAAGAGTTAC 
ACGCCCCCTG CTGGTTGCTG TCCCTCCATA CCACCTCAGT GCACTTGCGA CCTCCGTGCC 
TGCCGCAAGC CCCAGTGCCC GAGTGGACAG CATCCCGTTG CACTCAGTCA GGCCAGCGGA 
CAGCCGGGAG ACTGCTGCGA CGTCTTTGAG TGCCAGAAAG TTTCTCCCAA GTGTGTCCAC 
AATGGAAAGG AGTTCTCAGA GGGAGAGGTG TACCGCATGG ACCCCTGCTG GCTGTGTCAG 
TGCCGGGGAG GAATCTCCTT CTGCTCCAAG GCAGAGTGCG CTGAGCTGGA CTGTGAAAAT 
TTCTACATCC CTGAGGGCGA GTGTTGCCCT GTCTGCATAG ATGTGGAGTT ACTGTCGATG 
GACAGCACTA AGGCCAGCTG CTGGGTGAAT AATAAGCTGC GAGCACATGA GGAGCAGTGG 
AAAGAGGACG ACTGCACCTT CTGCCAGTGT GTGGATGGAG AACCGCACTG CACAGCCATG 
GCCTGCAAGC AGAGCTGCCA GAACCCTGTG AAGATCCCTG GGGAGTGTTG TCCTTTCTGT 
GAAGAACCAT CTTATGAAAC AGTCAGCCCC ATGCTATGTC CTCCTCTGGA AAACTGCTCC 
CTGTCAGGGA GTGACTGCCC ATATGGCTTC CACCAGGACA AAAATGGATG TCTTCTCTGC 
CAGTGTCTCA ACAATGACTC TTGCCCTGAC CTTGGAAAAT ACTGTTCCCT GCAATGCCCA 
ATGGGATACG AGAGGGATGA TTTTGGCTGC GAGGTGTGTG AATGCAGTAT ACCCCCACAC 
AAGTGCCGAC CTTTGACCTG CTCTAAGACC TGCCCATATG GATATGTGAA GAACAAACAT 
GGCTGTGAGA TGTGTCGCTG TGTCAAGTGT CCTCCCTTCA CCTGCGACAA GCACTGCGAC 
GATGGCTATC AACGGAACAA GAAGGGCTGC TCCATCTGCA TGTGTAAAGA AAGTGGCCAT 
GTGCCAAGCA CCACTCTCCC ACCTCCGATG CCCAGCTACT GCCTCACCTC CAATGGGCAC 
CGATATGAGG AAGGGGATGG CTGGCACGAC GGCTGCCGTG ACTGCTACTG CCACTCTGGA 
CGAGAGATGT GTGTGCTCAT TACCTGCCCT GTTCCCAGCT GTTCCCATCC AGTTGTCAAG 
CATGACCAGT GCTGTCCCAC CTGTGAGGAT GAATCAGGCA GCGGTCAACC AGAGGGGATG 
GACATGGTGG TGTGCAGAGC ACCTGGGGGC GAGTTTTATG TGGAGGGGGA GACCTGGAAC 
CTGGACGAGT GCACACGATG CACCTGTAGG AAGGGACGTG TCTTGTGCGA CACTGAAGTG 
TGTCCCCCAG CTCTCTGTCA GTCACCACTC AGGAACAAGG ACACCTGTTG TCATGTATGC 
CCAGATGAGA CTCTGAGCCC CCTGCTTCCA GTGAACAACA GCCAACAGGA GTACTGCATA 
ACCAGCGATG GAGACGTGCT GCTAGCGGGA GACTCCTGGA AGGCCAATGC CTGCACCAGC 
TGCACCTGCA ACAACGGCAC CATTCAGTGC TTCTCTCAGC GCTGTCCTGC TGCCAACTGC 
AGGGTGCCCG TTCTACGCAA GGGCCAATGC TGCCCACACT GTCTCGAGGT GACGACGTCA 
GTCCCTTTGA CCACCAGTGC CCCCAAGACA ACAGCCACTA CCACAGAGAA GAGGGTCACC 
ACTGTCACTG TACCACCCAT CAGTGCTGAT ACAGATGCAC CACATTTGGG TGACATCTAT 
CCCAGCAACA CAGCGATGGC CCTAATTTAC CAATCAGCTG CCTGGATCCT GGCAGGCCTC 
CTCTTGGCCA TTATCATTTT CCTCATTGTG GCGCTGCTCA TCAACAAGAA GAAGAAGTGG 
GTGCAGATGT CCTGCTACAG TGCGCCAAAG AAGACAGTGA TCCTGAAGAA ACATGTAAAC 
AAGAATTCAG TGGTCTATAT GGAACCTTCC AAGGAGAACA AATTTCAGAA TGTGAAGAAT 
GACTGCGGCA TCCACTTCTC TCCAGAAATG AGCTCCCCTT GCATTGACAA AATGACCATC 
CCCAGGGCCA AGCTGAGCAT GGGCAACGAC TGGACGCCAC GCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_028251716.1
CDS	420..3224
Translation	MFSKCSFVLLNVLFLVRALSAHECTPCNLRTCPVKPQHGCEGGRTLARDPCGCCDHCTRREWEPCGGQDWTHGYCALGLTCASLNKTGAATIPEIGVCKELPDHPDAGLEDERCPLMTGCDRAGGQCVCDARHSCLGSFTYLDQETCMKASKSDGRRHEHRERHREKYVGPSNPACMFSGCNLTAEGCVCESQSCHHHFAYINRSQCQEAAEELRCAGVTCPALQVPSSCPEGSVLTKSYTPPAGCCPSIPPQCTCDLRACRKPQCPSGQHPVALSQASGQPGDCCDVFECQKVSPKCVHNGKEFSEGEVYRMDPCWLCQCRGGISFCSKAECAELDCENFYIPEGECCPVCIDVELLSMDSTKASCWVNNKLRAHEEQWKEDDCTFCQCVDGEPHCTAMACKQSCQNPVKIPGECCPFCEEPSYETVSPMLCPPLENCSLSGSDCPYGFHQDKNGCLLCQCLNNDSCPDLGKYCSLQCPMGYERDDFGCEVCECSIPPHKCRPLTCSKTCPYGYVKNKHGCEMCRCVKCPPFTCDKHCDDGYQRNKKGCSICMCKESGHVPSTTLPPPMPSYCLTSNGHRYEEGDGWHDGCRDCYCHSGREMCVLITCPVPSCSHPVVKHDQCCPTCEDESGSGQPEGMDMVVCRAPGGEFYVEGETWNLDECTRCTCRKGRVLCDTEVCPPALCQSPLRNKDTCCHVCPDETLSPLLPVNNSQQEYCITSDGDVLLAGDSWKANACTSCTCNNGTIQCFSQRCPAANCRVPVLRKGQCCPHCLEVTTSVPLTTSAPKTTATTTEKRVTTVTVPPISADTDAPHLGDIYPSNTAMALIYQSAAWILAGLLLAIIIFLIVALLINKKKKWVQMSCYSAPKKTVILKKHVNKNSVVYMEPSKENKFQNVKNDCGIHFSPEMSSPCIDKMTIPRAKLSMGNDWTPR

Target ORF information:

RefSeq Version	XM_028395915.1
Organism	Parambassis ranga(Indian glassy fish)
Definition	Parambassis ranga cysteine rich transmembrane BMP regulator 1 (crim1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_028395915.1

ORF Insert Sequence:

ATGTTCAGCA AATGCTCTTT TGTGTTATTG AATGTCCTCT TTCTGGTGCG GGCGCTCTCG 
GCTCATGAGT GCACGCCGTG TAACCTCCGG ACTTGTCCCG TCAAACCCCA GCATGGTTGC 
GAGGGTGGCC GTACCTTGGC CAGGGATCCA TGCGGATGCT GTGATCACTG CACCCGGCGT 
GAGTGGGAGC CCTGCGGCGG TCAGGATTGG ACGCACGGCT ACTGCGCCCT GGGACTGACC 
TGCGCCTCCC TCAACAAAAC TGGAGCTGCT ACCATCCCTG AAATTGGAGT GTGTAAAGAA 
CTTCCTGACC ATCCAGATGC AGGTCTCGAG GATGAGCGCT GTCCCCTGAT GACGGGCTGT 
GACAGAGCAG GTGGCCAGTG TGTGTGTGAT GCACGTCACT CTTGCTTGGG CTCTTTCACA 
TACTTGGACC AGGAAACCTG CATGAAGGCC AGCAAGTCAG ATGGCCGGAG GCACGAGCAC 
CGGGAAAGGC ACAGAGAGAA GTACGTGGGA CCATCTAACC CAGCGTGTAT GTTCTCTGGG 
TGTAACTTGA CCGCCGAGGG TTGTGTGTGT GAGTCTCAGA GCTGCCATCA CCACTTCGCC 
TACATCAACC GCAGCCAGTG CCAGGAAGCC GCAGAGGAGC TGAGGTGTGC TGGTGTGACG 
TGTCCTGCTC TGCAGGTGCC CTCCTCCTGC CCTGAGGGCT CAGTCCTGAC CAAGAGTTAC 
ACGCCCCCTG CTGGTTGCTG TCCCTCCATA CCACCTCAGT GCACTTGCGA CCTCCGTGCC 
TGCCGCAAGC CCCAGTGCCC GAGTGGACAG CATCCCGTTG CACTCAGTCA GGCCAGCGGA 
CAGCCGGGAG ACTGCTGCGA CGTCTTTGAG TGCCAGAAAG TTTCTCCCAA GTGTGTCCAC 
AATGGAAAGG AGTTCTCAGA GGGAGAGGTG TACCGCATGG ACCCCTGCTG GCTGTGTCAG 
TGCCGGGGAG GAATCTCCTT CTGCTCCAAG GCAGAGTGCG CTGAGCTGGA CTGTGAAAAT 
TTCTACATCC CTGAGGGCGA GTGTTGCCCT GTCTGCATAG ATGTGGAGTT ACTGTCGATG 
GACAGCACTA AGGCCAGCTG CTGGGTGAAT AATAAGCTGC GAGCACATGA GGAGCAGTGG 
AAAGAGGACG ACTGCACCTT CTGCCAGTGT GTGGATGGAG AACCGCACTG CACAGCCATG 
GCCTGCAAGC AGAGCTGCCA GAACCCTGTG AAGATCCCTG GGGAGTGTTG TCCTTTCTGT 
GAAGAACCAT CTTATGAAAC AGTCAGCCCC ATGCTATGTC CTCCTCTGGA AAACTGCTCC 
CTGTCAGGGA GTGACTGCCC ATATGGCTTC CACCAGGACA AAAATGGATG TCTTCTCTGC 
CAGTGTCTCA ACAATGACTC TTGCCCTGAC CTTGGAAAAT ACTGTTCCCT GCAATGCCCA 
ATGGGATACG AGAGGGATGA TTTTGGCTGC GAGGTGTGTG AATGCAGTAT ACCCCCACAC 
AAGTGCCGAC CTTTGACCTG CTCTAAGACC TGCCCATATG GATATGTGAA GAACAAACAT 
GGCTGTGAGA TGTGTCGCTG TGTCAAGTGT CCTCCCTTCA CCTGCGACAA GCACTGCGAC 
GATGGCTATC AACGGAACAA GAAGGGCTGC TCCATCTGCA TGTGTAAAGA AAGTGGCCAT 
GTGCCAAGCA CCACTCTCCC ACCTCCGATG CCCAGCTACT GCCTCACCTC CAATGGGCAC 
CGATATGAGG AAGGGGATGG CTGGCACGAC GGCTGCCGTG ACTGCTACTG CCACTCTGGA 
CGAGAGATGT GTGTGCTCAT TACCTGCCCT GTTCCCAGCT GTTCCCATCC AGTTGTCAAG 
CATGACCAGT GCTGTCCCAC CTGTGAGGAT GAATCAGGCA GCGGTCAACC AGAGGGGATG 
GACATGGTGG TGTGCAGAGC ACCTGGGGGC GAGTTTTATG TGGAGGGGGA GACCTGGAAC 
CTGGACGAGT GCACACGATG CACCTGTAGG AAGGGACGTG TCTTGTGCGA CACTGAAGTG 
TGTCCCCCAG CTCTCTGTCA GTCACCACTC AGGAACAAGG ACACCTGTTG TCATGTATGC 
CCAGATGAGA CTCTGAGCCC CCTGCTTCCA GTGAACAACA GCCAACAGGA GTACTGCATA 
ACCAGCGATG GAGACGTGCT GCTAGCGGGA GACTCCTGGA AGGCCAATGC CTGCACCAGC 
TGCACCTGCA ACAACGGCAC CATTCAGTGC TTCTCTCAGC GCTGTCCTGC TGCCAACTGC 
AGGGTGCCCG TTCTACGCAA GGGCCAATGC TGCCCACACT GTCTCGAGGT GACGACGTCA 
GTCCCTTTGA CCACCAGTGC CCCCAAGACA ACAGCCACTA CCACAGAGAA GAGGGTCACC 
ACTGTCACTG TACCACCCAT CAGTGCTGAT ACAGATGCAC CACATTTGGG TGACATCTAT 
CCCAGCAACA CAGCGATGGC CCTAATTTAC CAATCAGCTG CCTGGATCCT GGCAGGCCTC 
CTCTTGGCCA TTATCATTTT CCTCATTGTG GCGCTGCTCA TCAACAAGAA GAAGAAGTGG 
GTGCAGATGT CCTGCTACAG TGCGCCAAAG AAGACAGTGA TCCTGAAGAA ACATGTAAAC 
AAGAATTCAG TGGTCTATAT GGAACCTTCC AAGGAGAACA AATTTCAGAA TGTGAAGAAT 
GACTGCGGCA TCCACTTCTC TCCAGAAATG AGCTCCCCTT GCATTGACAA AATGACCATC 
CCCAGGGCCA AGCTGAGCAT GGGCAACGAC TGGACGCCAC GCTAA