crim1 cDNA ORF clone,

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gRNAs

The following crim1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the crim1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OIn00976	XM_024273442.1 Latest version!	Oryzias melastigma cysteine rich transmembrane BMP regulator 1 (crim1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

crim1 ( XM_024273442.1 ) cDNA ORF clone, -> XP_024129210.1 Oryzias melastigma cysteine rich transmembrane BMP regulator 1 (crim1), mRNA.

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CloneID	OIn00976
Clone ID Related Accession (Same CDS sequence)	XM_024273442.1
Accession Version	XM_024273442.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2796bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-10
Organism	Oryzias melastigma(Indian medaka)
Product	cysteine-rich motor neuron 1 protein
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_020096073.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Oryzias melastigma Annotation Release 100 Annotation Version :: 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTTCACA AATTTTGCGC GGTGCTTCTG AGCGCGCTCT GTCTGGTGCG GGCGCTCTCG 
GCTCACGAGT GCACGCCCTG TAACCTCCGG ACCTGTCCCG TTCTACCGCC GCACGGCTGC 
GAAGGTGGCC GGAGCGTGGC CAGGGATCCA TGCGGATGCT GTGATCAGTG CGCGCGGCTG 
GAGTGGGAGC CGTGCGGCGG TCCGGACTGG ACGCTGGGCT ACTGCGCTCT GGGGCTGACC 
TGCGCCTCTG TCAACGGCAC GCGCGCCGCC ACCATACCTG ACACTGGAGT TTGTAAAGTT 
CTCCCGCAGC ATCCAGATGC AGGCCTCGAG GATGAGCGTT GCCCTCTAGT AACGGGCTGC 
GACAAAGCTG GAGATCAGTG CCTGTGCGAT GCCCGCCACT CCTGCTTGGG CTCCTTCACA 
TACCCGGACC AGGAAGCTTG CATGAAGTCC ATCAAGTCAG ATGCTCGGCG GCACGAGCAC 
CGGGAGAGGC ACAGAGAAAA GTACGTGGGA CCTCCCAACC CGGCGTGCAT GTTCTCGGGG 
TGTAACCTGA CCGCCGAGGG CTGCGTGTGC GAGTCTCAGA GCTGCCACCA CCACTTTGCC 
TACATCAACC GAAGCCAGTG CCAGGAAGCA GCAGACCAGC TGAGGTGTGC TGGGGTTAAA 
TGCCCCACTT TGCCAGTGCC TTCCTGCCCC GAAGACTCAG TCCTGACCAA GAGCTACACG 
CCCCCTGCTG GCTGCTGCCC CTCCATAACC CCCCAGTGTA CGTGTGACTT CCGGGCCTGC 
CAGAAGCCCC AGTGCCCGAG CGGAGAGCAC CGAGTCCTGC TCAGTCAAGC CAGCGGTCAG 
CCGGGAAACT GCTGCGATGT CTTTGAGTGT CGTAAAGTCT CTCCCAAATG TGTTCACAAT 
GGGAAGGAGC TCTCCGATGG AGAGGTGTAC CGCGTGGACT CCTGTTGGCT GTGCCAGTGC 
CGAGGAGGAA TCTCCTTCTG CTCCAAGGCG GAGTGCGCCG AGCTGGACTG CGAAAACTTC 
TACGTTCCTG AGGGCGAGTG CTGCCCCGTC TGCATAGATG TGGAGTTGTT GTCCGTGGAC 
AGCACCAAGG CCAGCTGCTG GGTGAACAAC AAGCTGCGAG CGCACGAGGA GCAGTGGAAA 
GAGGACGACT GCACCTTCTG CCAGTGTGTG GATGGAGACC CACAGTGCAC CGCCATGGCC 
TGCAAACAGA GCTGCCAAAA TCCTGTGAAG ATCCAAGGAG AGTGCTGCCC GTTCTGCGAA 
GAGCCAACCT ATGAAACCGT CAGCCCGATT CTCTGCCCTC CGTTGCAAAA CTGCTCCCTG 
TCCGTGAACG ACTGTCCCTT CGGCTTCTAT CAGGATGTAA ACGGATGTCT CCTCTGCCAG 
TGTCTCAACA ATGACTCCTG TCCAGACCTG GAAAAGCACT GCTCTCTCCG GTGTCCGCTG 
GGATTTGAGA AGGATGATTT TGGCTGTGAG GTGTGCGAAT GTGTCACCCC CATGCCCAAG 
TGCCGACCTC TGACCTGCAC AAAGACCTGC CCGTACGGAT ATGTGAGGAA CAAACACGGC 
TGTGAGATGT GTCGCTGTGT CAAGTGTCCG CCGTTCACCT GTGACAAGCA CTGCAGCGAC 
GGCTACCGGC AGAACAGGAA GGGATGCTCC GTGTGCATGT GCAAAGAAAG CCTCCCCAGC 
CCCGCTCTCC CAGCTCCAAC GCCCAGCTAT TGTCTCACCT CCAACGGTCA CCGCTATGAA 
GAAGGCGACA GCTGGCACGA TGGCTGCCGC GACTGTTTTT GCCACTCTGG GAGAGAGATG 
TGCGTGCTCA TTTCCTGCCC CATGCCCAGC TGCTCGCATC CTGTTGTCAA GGCGGACCAG 
TGCTGTCCTT CCTGTGAAGA TGAGTCAGGC AGCGGTCAGC CAGAGGGGAT GGACGTCATG 
GTCTGCAGAG CACCTGGAGG CGAGTTTTAT GTGGAGGGCG AGACCTGGAA CCTGGACGAG 
TGCACTCGCT GCACCTGCAG GAAGGGCCGT GTCCTGTGCG ACACCGAGGT GTGTCCTCCG 
GCTCTCTGCC AGACGCCCAT AAGAAACAAG GATACCTGCT GCCACGTTTG CCCAGAGGAG 
ACTCTGGGGC CTTTACTGCC AGTGAACAGA AGTGAGCAGC AGTACTGCCT GACTGTCGAA 
GGAGAGGTGC TGCTTGCTGG TGACTCCTGG AGGGCGAACG CCTGCACCAG CTGTACTTGC 
AACAACGGGA CCATTCAGTG TTTCTCCCAG CGCTGTCCAG CCGCCAACTG CAGGGTACCA 
GTTCTACGCA AGGGCCAATG CTGCCCGCAC TGTCTCGAGG TGACCACATC AGTGCCAGTA 
AAAGTGACCG CCAATACCGC CACCACGACA CTGGAGATTG TATTTGAGAT GACCACAGTC 
ACTGTACCGC CCATCACTGC TGACACAGAT GGGCTGGAGT TTGGTGATAA TTACCCCAGC 
CAAGCCGAGA TGGCCCTGGT CTACCAATCA GCAGCCTGGA TTCTGGCGGG CCTCCTCTTG 
GCCATCATCA TCTTCCTCTT AGTTGCGTTT CTCATCAACA AGAAGAAGAA ATGGGTGCAG 
ATGTCCTGCT ATAGCGCACC AAAGAAGACG GTGATCCTGA AAAAGCACAT AAACAAGAAC 
TCCGTGGTCT ACATGGAGCC TTCCAAGGAG AACAAGTTCC AGAACGTGAA GAACGACTGC 
GGCGTCATCC ACTTCTCTCC GGACATGAGT TCCCCCTGCA AAGACAGGAT GACCATCCCC 
AGGGCCAAAC TAGCCAACGA CTGGACGTCA CGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024129210.1
CDS	308..3103
Translation	MFHKFCAVLLSALCLVRALSAHECTPCNLRTCPVLPPHGCEGGRSVARDPCGCCDQCARLEWEPCGGPDWTLGYCALGLTCASVNGTRAATIPDTGVCKVLPQHPDAGLEDERCPLVTGCDKAGDQCLCDARHSCLGSFTYPDQEACMKSIKSDARRHEHRERHREKYVGPPNPACMFSGCNLTAEGCVCESQSCHHHFAYINRSQCQEAADQLRCAGVKCPTLPVPSCPEDSVLTKSYTPPAGCCPSITPQCTCDFRACQKPQCPSGEHRVLLSQASGQPGNCCDVFECRKVSPKCVHNGKELSDGEVYRVDSCWLCQCRGGISFCSKAECAELDCENFYVPEGECCPVCIDVELLSVDSTKASCWVNNKLRAHEEQWKEDDCTFCQCVDGDPQCTAMACKQSCQNPVKIQGECCPFCEEPTYETVSPILCPPLQNCSLSVNDCPFGFYQDVNGCLLCQCLNNDSCPDLEKHCSLRCPLGFEKDDFGCEVCECVTPMPKCRPLTCTKTCPYGYVRNKHGCEMCRCVKCPPFTCDKHCSDGYRQNRKGCSVCMCKESLPSPALPAPTPSYCLTSNGHRYEEGDSWHDGCRDCFCHSGREMCVLISCPMPSCSHPVVKADQCCPSCEDESGSGQPEGMDVMVCRAPGGEFYVEGETWNLDECTRCTCRKGRVLCDTEVCPPALCQTPIRNKDTCCHVCPEETLGPLLPVNRSEQQYCLTVEGEVLLAGDSWRANACTSCTCNNGTIQCFSQRCPAANCRVPVLRKGQCCPHCLEVTTSVPVKVTANTATTTLEIVFEMTTVTVPPITADTDGLEFGDNYPSQAEMALVYQSAAWILAGLLLAIIIFLLVAFLINKKKKWVQMSCYSAPKKTVILKKHINKNSVVYMEPSKENKFQNVKNDCGVIHFSPDMSSPCKDRMTIPRAKLANDWTSR

Target ORF information:

RefSeq Version	XM_024273442.1
Organism	Oryzias melastigma(Indian medaka)
Definition	Oryzias melastigma cysteine rich transmembrane BMP regulator 1 (crim1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024273442.1

ORF Insert Sequence:

ATGTTTCACA AATTTTGCGC GGTGCTTCTG AGCGCGCTCT GTCTGGTGCG GGCGCTCTCG 
GCTCACGAGT GCACGCCCTG TAACCTCCGG ACCTGTCCCG TTCTACCGCC GCACGGCTGC 
GAAGGTGGCC GGAGCGTGGC CAGGGATCCA TGCGGATGCT GTGATCAGTG CGCGCGGCTG 
GAGTGGGAGC CGTGCGGCGG TCCGGACTGG ACGCTGGGCT ACTGCGCTCT GGGGCTGACC 
TGCGCCTCTG TCAACGGCAC GCGCGCCGCC ACCATACCTG ACACTGGAGT TTGTAAAGTT 
CTCCCGCAGC ATCCAGATGC AGGCCTCGAG GATGAGCGTT GCCCTCTAGT AACGGGCTGC 
GACAAAGCTG GAGATCAGTG CCTGTGCGAT GCCCGCCACT CCTGCTTGGG CTCCTTCACA 
TACCCGGACC AGGAAGCTTG CATGAAGTCC ATCAAGTCAG ATGCTCGGCG GCACGAGCAC 
CGGGAGAGGC ACAGAGAAAA GTACGTGGGA CCTCCCAACC CGGCGTGCAT GTTCTCGGGG 
TGTAACCTGA CCGCCGAGGG CTGCGTGTGC GAGTCTCAGA GCTGCCACCA CCACTTTGCC 
TACATCAACC GAAGCCAGTG CCAGGAAGCA GCAGACCAGC TGAGGTGTGC TGGGGTTAAA 
TGCCCCACTT TGCCAGTGCC TTCCTGCCCC GAAGACTCAG TCCTGACCAA GAGCTACACG 
CCCCCTGCTG GCTGCTGCCC CTCCATAACC CCCCAGTGTA CGTGTGACTT CCGGGCCTGC 
CAGAAGCCCC AGTGCCCGAG CGGAGAGCAC CGAGTCCTGC TCAGTCAAGC CAGCGGTCAG 
CCGGGAAACT GCTGCGATGT CTTTGAGTGT CGTAAAGTCT CTCCCAAATG TGTTCACAAT 
GGGAAGGAGC TCTCCGATGG AGAGGTGTAC CGCGTGGACT CCTGTTGGCT GTGCCAGTGC 
CGAGGAGGAA TCTCCTTCTG CTCCAAGGCG GAGTGCGCCG AGCTGGACTG CGAAAACTTC 
TACGTTCCTG AGGGCGAGTG CTGCCCCGTC TGCATAGATG TGGAGTTGTT GTCCGTGGAC 
AGCACCAAGG CCAGCTGCTG GGTGAACAAC AAGCTGCGAG CGCACGAGGA GCAGTGGAAA 
GAGGACGACT GCACCTTCTG CCAGTGTGTG GATGGAGACC CACAGTGCAC CGCCATGGCC 
TGCAAACAGA GCTGCCAAAA TCCTGTGAAG ATCCAAGGAG AGTGCTGCCC GTTCTGCGAA 
GAGCCAACCT ATGAAACCGT CAGCCCGATT CTCTGCCCTC CGTTGCAAAA CTGCTCCCTG 
TCCGTGAACG ACTGTCCCTT CGGCTTCTAT CAGGATGTAA ACGGATGTCT CCTCTGCCAG 
TGTCTCAACA ATGACTCCTG TCCAGACCTG GAAAAGCACT GCTCTCTCCG GTGTCCGCTG 
GGATTTGAGA AGGATGATTT TGGCTGTGAG GTGTGCGAAT GTGTCACCCC CATGCCCAAG 
TGCCGACCTC TGACCTGCAC AAAGACCTGC CCGTACGGAT ATGTGAGGAA CAAACACGGC 
TGTGAGATGT GTCGCTGTGT CAAGTGTCCG CCGTTCACCT GTGACAAGCA CTGCAGCGAC 
GGCTACCGGC AGAACAGGAA GGGATGCTCC GTGTGCATGT GCAAAGAAAG CCTCCCCAGC 
CCCGCTCTCC CAGCTCCAAC GCCCAGCTAT TGTCTCACCT CCAACGGTCA CCGCTATGAA 
GAAGGCGACA GCTGGCACGA TGGCTGCCGC GACTGTTTTT GCCACTCTGG GAGAGAGATG 
TGCGTGCTCA TTTCCTGCCC CATGCCCAGC TGCTCGCATC CTGTTGTCAA GGCGGACCAG 
TGCTGTCCTT CCTGTGAAGA TGAGTCAGGC AGCGGTCAGC CAGAGGGGAT GGACGTCATG 
GTCTGCAGAG CACCTGGAGG CGAGTTTTAT GTGGAGGGCG AGACCTGGAA CCTGGACGAG 
TGCACTCGCT GCACCTGCAG GAAGGGCCGT GTCCTGTGCG ACACCGAGGT GTGTCCTCCG 
GCTCTCTGCC AGACGCCCAT AAGAAACAAG GATACCTGCT GCCACGTTTG CCCAGAGGAG 
ACTCTGGGGC CTTTACTGCC AGTGAACAGA AGTGAGCAGC AGTACTGCCT GACTGTCGAA 
GGAGAGGTGC TGCTTGCTGG TGACTCCTGG AGGGCGAACG CCTGCACCAG CTGTACTTGC 
AACAACGGGA CCATTCAGTG TTTCTCCCAG CGCTGTCCAG CCGCCAACTG CAGGGTACCA 
GTTCTACGCA AGGGCCAATG CTGCCCGCAC TGTCTCGAGG TGACCACATC AGTGCCAGTA 
AAAGTGACCG CCAATACCGC CACCACGACA CTGGAGATTG TATTTGAGAT GACCACAGTC 
ACTGTACCGC CCATCACTGC TGACACAGAT GGGCTGGAGT TTGGTGATAA TTACCCCAGC 
CAAGCCGAGA TGGCCCTGGT CTACCAATCA GCAGCCTGGA TTCTGGCGGG CCTCCTCTTG 
GCCATCATCA TCTTCCTCTT AGTTGCGTTT CTCATCAACA AGAAGAAGAA ATGGGTGCAG 
ATGTCCTGCT ATAGCGCACC AAAGAAGACG GTGATCCTGA AAAAGCACAT AAACAAGAAC 
TCCGTGGTCT ACATGGAGCC TTCCAAGGAG AACAAGTTCC AGAACGTGAA GAACGACTGC 
GGCGTCATCC ACTTCTCTCC GGACATGAGT TCCCCCTGCA AAGACAGGAT GACCATCCCC 
AGGGCCAAAC TAGCCAACGA CTGGACGTCA CGCTAA