BCKDHA cDNA ORF clone, Ornithorhynchus anatinus(platypus)

Gene
Clones
gRNAs

Summary

Gene Symbol	BCKDHA
Entrez Gene ID	100088094
Full Name	branched chain keto acid dehydrogenase E1, alpha polypeptide
Gene Type	protein-coding
Organism	Ornithorhynchus anatinus(platypus)

mRNA and Protein(s)

mRNA	Protein	Name
XM_016226271.1	XP_016081757.1	2-oxoisovalerate dehydrogenase subunit alpha, mitochondrial
XM_029066359.1	XP_028922192.1	2-oxoisovalerate dehydrogenase subunit alpha, mitochondrial

Pathways from BioSystems
Gene Ontology

The following BCKDHA gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the BCKDHA cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OOc130157	XM_029066359.1 Latest version!	Ornithorhynchus anatinus branched chain keto acid dehydrogenase E1 subunit alpha (BCKDHA), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

BCKDHA ( XM_029066359.1 ) cDNA ORF clone, Ornithorhynchus anatinus(platypus) -> XP_028922192.1 Ornithorhynchus anatinus branched chain keto acid dehydrogenase E1 subunit alpha (BCKDHA), mRNA.

Price & Availability↑

CloneID	OOc130157
Clone ID Related Accession (Same CDS sequence)	XM_029066359.1
Accession Version	XM_029066359.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1377bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-28
Organism	Ornithorhynchus anatinus(platypus)
Product	2-oxoisovalerate dehydrogenase subunit alpha, mitochondrial
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_041732.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Ornithorhynchus anatinus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCCA AGATGGCGTC GGGGCGCCTG GCCTGGGCGC TGAGGGGCGG CCTCCGCCGG 
GGCTGGCGCG GCCTGGGGGC CCGGCCGGGG CCTCGCTCCC CGCCGCCCTC ATCACCACCG 
CCCGGGGCCA GGGGTCTGAC CGCCTCCGCC CCCCGGCAGC AGCAGGAAGA ATTCCCATCG 
CTGGAAGAGA AGCCCCAGTT CCCGGGGGCC TCGGCCGAAT TCGTGGACAA GCTGGACTTT 
ATCCAGCCCA ACGTCATTTC CGGCATCCCC GTCTACCGGG TCATGGACCG GCAGGGCCAG 
ATCGTGAACC CCGAGGAAGA CCCCCAGCTG CCCCGGGAAC AGGTGCTGAA GTTCTACAGG 
AGTATGACGC TCCTCAACAC CATGGACCGC ATCCTCTATG AGTCTCAGAG GCAGGGCCGC 
ATCTCCTTCT ACATGACCAA CTACGGCGAG GAGGGCACCC ACGTCGGTAG CGCCGCCGCC 
CTGGACGACA CCGACCTGGT GTTCGGGCAG TACCGAGAGG CGGGGGTGCT GATGTACCGC 
GGCTACCCGC TGGACCTCTT CATGTCCCAG TGCTACGGTA ACGCCAGTGA CCCGGGCAAA 
GGCCGCCAGA TGCCCGTGCA TTACGGCTGC AAGGACCTGC ACTTCGTCAC CATCTCCTCC 
CCGCTGGCTA CCCAGATCCC CCAAGCGGTG GGCGCGGCCT ACGCCGCCAA GAGGGAGAAC 
GCCAACCGGG CCGTCATCTG CTACTTCGGG GAGGGGGCGG CCAGCGAGGG AGACGCCCAC 
GCGGGCTTCA ACTTCGCCGC CACCCTGGAG TGCCCCATCA TCTTCTTCTG CCGCAACAAC 
GGCTACGCCA TCTCCACGCC GACCTCGGAG CAGTACCGCG GGGACGGCAT CGCCGCCAGA 
GGCCCCGGCT ACGGGATCAT GTCGATCCGG GTGGACGGCA ACGACGTGTT TGCCGTCTAC 
AACGCCACCA GGGAAGCCCG GCGCCGGGCC GTGGCCGAAA ACCAGCCCTT CCTCATCGAG 
GCCATGACGT ACAGGATCGG GCACCACAGC ACGAGCGACG ACAGCTCGGC CTACCGCTCG 
GTGGACGAGG TCAACTACTG GGACAAGCAG GACCACCCCA TCTCCCGCCT GCGCCATCAC 
CTGCTCAGCA AGGGCTGGTG GGGGGAGGAA GAGGAGAAGA CCTGGAGGAA GCAGTCGCGC 
AAGCTGGTGA TGGAGGCCTT CGAGGCGGCG GAACGGAAGC TGAAGCCCAA CCTCAACCTG 
CTGTTCTCGG ACGTGTACCG CGAGATGCCC GCCCACCTGC AGAAGCAGCA GGACGCCCTG 
GCCCGCCACC TCAAGACCTA CGGCGAGCAC TACCCCCTGG ACCACTTTGA GCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_028922192.1
CDS	42..1418
Translation	MAAKMASGRLAWALRGGLRRGWRGLGARPGPRSPPPSSPPPGARGLTASAPRQQQEEFPSLEEKPQFPGASAEFVDKLDFIQPNVISGIPVYRVMDRQGQIVNPEEDPQLPREQVLKFYRSMTLLNTMDRILYESQRQGRISFYMTNYGEEGTHVGSAAALDDTDLVFGQYREAGVLMYRGYPLDLFMSQCYGNASDPGKGRQMPVHYGCKDLHFVTISSPLATQIPQAVGAAYAAKRENANRAVICYFGEGAASEGDAHAGFNFAATLECPIIFFCRNNGYAISTPTSEQYRGDGIAARGPGYGIMSIRVDGNDVFAVYNATREARRRAVAENQPFLIEAMTYRIGHHSTSDDSSAYRSVDEVNYWDKQDHPISRLRHHLLSKGWWGEEEEKTWRKQSRKLVMEAFEAAERKLKPNLNLLFSDVYREMPAHLQKQQDALARHLKTYGEHYPLDHFEQ

Target ORF information:

RefSeq Version	XM_029066359.1
Organism	Ornithorhynchus anatinus(platypus)
Definition	Ornithorhynchus anatinus branched chain keto acid dehydrogenase E1 subunit alpha (BCKDHA), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_029066359.1

ORF Insert Sequence:

ATGGCGGCCA AGATGGCGTC GGGGCGCCTG GCCTGGGCGC TGAGGGGCGG CCTCCGCCGG 
GGCTGGCGCG GCCTGGGGGC CCGGCCGGGG CCTCGCTCCC CGCCGCCCTC ATCACCACCG 
CCCGGGGCCA GGGGTCTGAC CGCCTCCGCC CCCCGGCAGC AGCAGGAAGA ATTCCCATCG 
CTGGAAGAGA AGCCCCAGTT CCCGGGGGCC TCGGCCGAAT TCGTGGACAA GCTGGACTTT 
ATCCAGCCCA ACGTCATTTC CGGCATCCCC GTCTACCGGG TCATGGACCG GCAGGGCCAG 
ATCGTGAACC CCGAGGAAGA CCCCCAGCTG CCCCGGGAAC AGGTGCTGAA GTTCTACAGG 
AGTATGACGC TCCTCAACAC CATGGACCGC ATCCTCTATG AGTCTCAGAG GCAGGGCCGC 
ATCTCCTTCT ACATGACCAA CTACGGCGAG GAGGGCACCC ACGTCGGTAG CGCCGCCGCC 
CTGGACGACA CCGACCTGGT GTTCGGGCAG TACCGAGAGG CGGGGGTGCT GATGTACCGC 
GGCTACCCGC TGGACCTCTT CATGTCCCAG TGCTACGGTA ACGCCAGTGA CCCGGGCAAA 
GGCCGCCAGA TGCCCGTGCA TTACGGCTGC AAGGACCTGC ACTTCGTCAC CATCTCCTCC 
CCGCTGGCTA CCCAGATCCC CCAAGCGGTG GGCGCGGCCT ACGCCGCCAA GAGGGAGAAC 
GCCAACCGGG CCGTCATCTG CTACTTCGGG GAGGGGGCGG CCAGCGAGGG AGACGCCCAC 
GCGGGCTTCA ACTTCGCCGC CACCCTGGAG TGCCCCATCA TCTTCTTCTG CCGCAACAAC 
GGCTACGCCA TCTCCACGCC GACCTCGGAG CAGTACCGCG GGGACGGCAT CGCCGCCAGA 
GGCCCCGGCT ACGGGATCAT GTCGATCCGG GTGGACGGCA ACGACGTGTT TGCCGTCTAC 
AACGCCACCA GGGAAGCCCG GCGCCGGGCC GTGGCCGAAA ACCAGCCCTT CCTCATCGAG 
GCCATGACGT ACAGGATCGG GCACCACAGC ACGAGCGACG ACAGCTCGGC CTACCGCTCG 
GTGGACGAGG TCAACTACTG GGACAAGCAG GACCACCCCA TCTCCCGCCT GCGCCATCAC 
CTGCTCAGCA AGGGCTGGTG GGGGGAGGAA GAGGAGAAGA CCTGGAGGAA GCAGTCGCGC 
AAGCTGGTGA TGGAGGCCTT CGAGGCGGCG GAACGGAAGC TGAAGCCCAA CCTCAACCTG 
CTGTTCTCGG ACGTGTACCG CGAGATGCCC GCCCACCTGC AGAAGCAGCA GGACGCCCTG 
GCCCGCCACC TCAAGACCTA CGGCGAGCAC TACCCCCTGG ACCACTTTGA GCAGTGA