csf1 cDNA ORF clone, Oncorhynchus mykiss(rainbow trout)

Gene
Clones
gRNAs

Summary

Gene Symbol	csf1
Entrez Gene ID	100136089
Full Name	macrophage colony-stimulating factor precursor
Gene Type	protein-coding
Organism	Oncorhynchus mykiss(rainbow trout)

mRNA and Protein(s)

mRNA	Protein	Name
XM_021567398.1	XP_021423073.1	macrophage colony-stimulating factor precursor isoform X1
NM_001124394.1	NP_001117866.1	macrophage colony-stimulating factor precursor
XM_021567399.1	XP_021423074.1	macrophage colony-stimulating factor precursor isoform X2

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Identification of IL-34 in teleost fish: differential expression of rainbow trout IL-34, MCSF1 and MCSF2, ligands of the MCSF receptor.
Wang T, Kono T, Monte MM, Kuse H, Costa MM, Korenaga H, Maehr T, Husain M, Sakai M, Secombes CJ
Molecular immunology53(4)398-409(2013 Apr)

Characterization of the rainbow trout transcriptome using Sanger and 454-pyrosequencing approaches.
Salem M, Rexroad CE 3rd, Wang J, Thorgaard GH, Yao J
BMC genomics11564(2010 Oct)

Two macrophage colony-stimulating factor genes exist in fish that differ in gene organization and are differentially expressed.
Wang T, Hanington PC, Belosevic M, Secombes CJ
Journal of immunology (Baltimore, Md. : 1950)181(5)3310-22(2008 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The expression of MCSF1 showed great variance in different tissues and cell lines, suggesting a role in the differentiation and maintenance of specific macrophage lineages in specific locations.
Title: Identification of IL-34 in teleost fish: differential expression of rainbow trout IL-34, MCSF1 and MCSF2, ligands of the MCSF receptor.

The following csf1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the csf1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OOf01168	NM_001124394.1 Latest version!	Oncorhynchus mykiss macrophage colony-stimulating factor precursor (csf1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OOf16743	XM_021567398.1 Latest version!	Oncorhynchus mykiss macrophage colony-stimulating factor precursor (csf1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00
OOf16744	XM_021567399.1 Latest version!	Oncorhynchus mykiss macrophage colony-stimulating factor precursor (csf1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

csf1 ( NM_001124394.1 ) cDNA ORF clone, Oncorhynchus mykiss(rainbow trout) -> NP_001117866.1 Oncorhynchus mykiss macrophage colony-stimulating factor precursor (csf1), mRNA.

Price & Availability↑

CloneID	OOf01168
Clone ID Related Accession (Same CDS sequence)	NM_001124394.1
Accession Version	NM_001124394.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1782bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-07-09
Organism	Oncorhynchus mykiss(rainbow trout)
Product	macrophage colony-stimulating factor precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AJ555867.1. ##Evidence-Data-START## Transcript exon combination :: AJ555867.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00631607, SAMN04511055 [ECO:0000348] ##Evidence-Data-END##

ATGAACACAC ACAAACCAGC CCACAAAGCC AAGGCTAGGC ATCTGTGCTT CCTCTTCCTC 
ATGTGCTTCC CTTTGGCGTG GGGTGGCGTC CCTGGTCCAT GTCGGCACTC TGTGACCAAG 
GGTCACCTCC TCAACCTCAA CTGCCTGATT GATAATCAGT TGGAGAATGG CTGGTCAATA 
ACCTACGTAT TCACTGAGCG CCAGAGTTTG AGTGAAGTCT GTTACATCAA AGCAGCATTT 
CCACAAATCC TGGAGCTTCT GAACACACAC TTCAAGTATG TCAGGAAATC GGACAACGGG 
CGCTATGTCA AACTCCTGAA AAAGGTCATA TATGACCTCT ACTCCCAAAA CTGCATACCT 
GAAATTAATG AGGAGATTGA GGACACCCCA TTGAAGTTTG TCAGAATGCA CAGCACCTCG 
CCCAGAGAGG CTCTGACGAA AGCCCGGGGG GTCATTGAGA TGTGCATGAC CCTGATGACC 
AAGAGCAACG GGCCCGTCGA CTGGAACTGT GAGGAAGAGT ATGCAGAGGA TTACCAAGAA 
TCCACCACTG CCCTCCCCAC CCCCACATCA GCTGAACCTG AACCTGAACG CCAGTGTACG 
TGTCCGACAG TGGGTCCGGT AACCCCTGAT GTCTCCCTGG TGTCCCACAG CGTATGGAGC 
AGTCCCCCAC AGCCCACCCT CTCCCAACTC ACCTCGATTG TGCCTCCCAG GCTGACTCAT 
CCATCCCTGG GTACCAAGGG CACTGAGCCC CGGGTGTTCC TGGTGAGTTC TGGTACCGTA 
AGACCCCCCT TCCCCAGGGA GCTGCAGCGG ACTGTCCCCG ATGGACGTTC ACTTGGAGGG 
GGCAGAGAGG GGGATATACA CTGGGACTTT CGTCCACCTT CACCCAATGG AACTGAGTCA 
GATTCGGGAC TCTACCAGGC AGTTGCTGAC ACCCCGTCTG CCACGAATGA ACCTTCCCCG 
TCTTCCCCTC CTAGTCTGGT TCTTCTGGGG ACCGTTGGCT CCCATGACGA GACATTTTAC 
TACCCCCAGA CTGAGAGGAT AAGGGAGACT TCCACAGCCC GACCAGCAGG GAGAGGAGCA 
GCAGGCTCAC CCCACAGTCA CTTCGCTGTT GCTGGGGCCA AGCTGGTCAC ACCAGTCCCT 
TTCCTTTACA AGTTGATTGG CATCACCCCC AGTAGTGATG AGAGCACTGC CTATGTGTTG 
GCCAAGAGGT CGCTGGATGC CAGGAATTAT GGAATACTTC AGGATTGGGT TTCAACAGAG 
ACCCCTCATC TAGACGACAC TAGAGAAAAA TACCCAGGCA CAGAGAGATA TTTACATGAC 
TGGAACCCTC CGATTGAAAG CACTACGACC GAAGCATCTC CACGTTTGAA GATGACAGTG 
GAGGAGCCAG ACAAACTACA CACCACTACA GAGGCACACG ACCAAGTCAC GAAGACTGAG 
CCAAACCATC CTAGGACATC CAATAAGGAA GTATTCAGCA CAGATATCCT GTCAGAGAGG 
ACAGCTGCAG ACGATGCTCC TATTTCAGAA AGGTCCAGTG AAGGCTCAGT GAAGGAATTC 
CGACACGGGA AAACTGGTCA TTCCGATTTA AGCATCTCCT ACCAGACAGC CTTTATCATT 
GCAGCAGTGT GCAGCGGACC AATCCTGATC ATTACCCTAT TCTGTCTCAG TGAGAAGAAG 
AGACTCCAAG CCCTTATCCA CAGTACCAGC ATAATTGAAA ACCAGAGGGC ATGCAGCAGC 
ATTGAGGACA TTGAGCTACA ATATTGTAAA GCCAAGGATT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001117866.1
CDS	190..1971
Translation	MNTHKPAHKAKARHLCFLFLMCFPLAWGGVPGPCRHSVTKGHLLNLNCLIDNQLENGWSITYVFTERQSLSEVCYIKAAFPQILELLNTHFKYVRKSDNGRYVKLLKKVIYDLYSQNCIPEINEEIEDTPLKFVRMHSTSPREALTKARGVIEMCMTLMTKSNGPVDWNCEEEYAEDYQESTTALPTPTSAEPEPERQCTCPTVGPVTPDVSLVSHSVWSSPPQPTLSQLTSIVPPRLTHPSLGTKGTEPRVFLVSSGTVRPPFPRELQRTVPDGRSLGGGREGDIHWDFRPPSPNGTESDSGLYQAVADTPSATNEPSPSSPPSLVLLGTVGSHDETFYYPQTERIRETSTARPAGRGAAGSPHSHFAVAGAKLVTPVPFLYKLIGITPSSDESTAYVLAKRSLDARNYGILQDWVSTETPHLDDTREKYPGTERYLHDWNPPIESTTTEASPRLKMTVEEPDKLHTTTEAHDQVTKTEPNHPRTSNKEVFSTDILSERTAADDAPISERSSEGSVKEFRHGKTGHSDLSISYQTAFIIAAVCSGPILIITLFCLSEKKRLQALIHSTSIIENQRACSSIEDIELQYCKAKD

Target ORF information:

RefSeq Version	NM_001124394.1
Organism	Oncorhynchus mykiss(rainbow trout)
Definition	Oncorhynchus mykiss macrophage colony-stimulating factor precursor (csf1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001124394.1

ORF Insert Sequence:

ATGAACACAC ACAAACCAGC CCACAAAGCC AAGGCTAGGC ATCTGTGCTT CCTCTTCCTC 
ATGTGCTTCC CTTTGGCGTG GGGTGGCGTC CCTGGTCCAT GTCGGCACTC TGTGACCAAG 
GGTCACCTCC TCAACCTCAA CTGCCTGATT GATAATCAGT TGGAGAATGG CTGGTCAATA 
ACCTACGTAT TCACTGAGCG CCAGAGTTTG AGTGAAGTCT GTTACATCAA AGCAGCATTT 
CCACAAATCC TGGAGCTTCT GAACACACAC TTCAAGTATG TCAGGAAATC GGACAACGGG 
CGCTATGTCA AACTCCTGAA AAAGGTCATA TATGACCTCT ACTCCCAAAA CTGCATACCT 
GAAATTAATG AGGAGATTGA GGACACCCCA TTGAAGTTTG TCAGAATGCA CAGCACCTCG 
CCCAGAGAGG CTCTGACGAA AGCCCGGGGG GTCATTGAGA TGTGCATGAC CCTGATGACC 
AAGAGCAACG GGCCCGTCGA CTGGAACTGT GAGGAAGAGT ATGCAGAGGA TTACCAAGAA 
TCCACCACTG CCCTCCCCAC CCCCACATCA GCTGAACCTG AACCTGAACG CCAGTGTACG 
TGTCCGACAG TGGGTCCGGT AACCCCTGAT GTCTCCCTGG TGTCCCACAG CGTATGGAGC 
AGTCCCCCAC AGCCCACCCT CTCCCAACTC ACCTCGATTG TGCCTCCCAG GCTGACTCAT 
CCATCCCTGG GTACCAAGGG CACTGAGCCC CGGGTGTTCC TGGTGAGTTC TGGTACCGTA 
AGACCCCCCT TCCCCAGGGA GCTGCAGCGG ACTGTCCCCG ATGGACGTTC ACTTGGAGGG 
GGCAGAGAGG GGGATATACA CTGGGACTTT CGTCCACCTT CACCCAATGG AACTGAGTCA 
GATTCGGGAC TCTACCAGGC AGTTGCTGAC ACCCCGTCTG CCACGAATGA ACCTTCCCCG 
TCTTCCCCTC CTAGTCTGGT TCTTCTGGGG ACCGTTGGCT CCCATGACGA GACATTTTAC 
TACCCCCAGA CTGAGAGGAT AAGGGAGACT TCCACAGCCC GACCAGCAGG GAGAGGAGCA 
GCAGGCTCAC CCCACAGTCA CTTCGCTGTT GCTGGGGCCA AGCTGGTCAC ACCAGTCCCT 
TTCCTTTACA AGTTGATTGG CATCACCCCC AGTAGTGATG AGAGCACTGC CTATGTGTTG 
GCCAAGAGGT CGCTGGATGC CAGGAATTAT GGAATACTTC AGGATTGGGT TTCAACAGAG 
ACCCCTCATC TAGACGACAC TAGAGAAAAA TACCCAGGCA CAGAGAGATA TTTACATGAC 
TGGAACCCTC CGATTGAAAG CACTACGACC GAAGCATCTC CACGTTTGAA GATGACAGTG 
GAGGAGCCAG ACAAACTACA CACCACTACA GAGGCACACG ACCAAGTCAC GAAGACTGAG 
CCAAACCATC CTAGGACATC CAATAAGGAA GTATTCAGCA CAGATATCCT GTCAGAGAGG 
ACAGCTGCAG ACGATGCTCC TATTTCAGAA AGGTCCAGTG AAGGCTCAGT GAAGGAATTC 
CGACACGGGA AAACTGGTCA TTCCGATTTA AGCATCTCCT ACCAGACAGC CTTTATCATT 
GCAGCAGTGT GCAGCGGACC AATCCTGATC ATTACCCTAT TCTGTCTCAG TGAGAAGAAG 
AGACTCCAAG CCCTTATCCA CAGTACCAGC ATAATTGAAA ACCAGAGGGC ATGCAGCAGC 
ATTGAGGACA TTGAGCTACA ATATTGTAAA GCCAAGGATT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

csf1 ( XM_021567398.1 ) cDNA ORF clone, Oncorhynchus mykiss(rainbow trout) -> XP_021423073.1 Oncorhynchus mykiss macrophage colony-stimulating factor precursor (csf1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OOf16743
Clone ID Related Accession (Same CDS sequence)	XM_021567398.1
Accession Version	XM_021567398.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1839bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-26
Organism	Oncorhynchus mykiss(rainbow trout)
Product	macrophage colony-stimulating factor precursor isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_035093.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Oncorhynchus mykiss Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCCCTA CTCTGGAACC TGGGCATTTT GGGTTACTGG TATCATTCCA AGATTTTTTG 
TTAGCGGTAA CAACTGATGG GCTGCTAATA GCTAGGCATC TGTGCTTCCT CTTCCTCATG 
TGCTTCCCTT TGGCGTGGGG TGGCGTCCCT GGTCCATGTC GGCACTCTGT GACCAAGGGT 
CACCTCCTCA ACCTCAACTG CCTGATTGAT AATCAGTTGG AGAATGGCTG GTCAATAACC 
TACGTATTCA CTGAGCGCCA GAGTTTGAGT GAAGTCTGTT ACATCAAAGC AGCATTTCCA 
CAAATCCTGG AGCTTCTGAA CACACACTTC AAGTATGTCA GGAAATCGGA CAACGGGCGC 
TATGTCAAAC TCCTGAAAAA GGTCATATAT GACCTCTACT CCCAAAACTG CATACCAGAA 
ATTAATGAGG AGATTGAGGA CACCCCATTG AAGTTTGTCA GAATGCACAG CACCTCGCCC 
AGAGAGGCTC TGACGAAAGC CCGGGGGGTC ATTGAGATGT ACATGACCCT GATGACCAAG 
AGCAACGGGC CCGTCGACTG GAACTGTGAG GAAGAGTATG CAGAGGATTA CCAAGAATCC 
ACCACTGCCC TCCCCACCCC CACCGCAGCT GAACCTGAAC CTGAACGCCA GTGTACGTGT 
CCGACAGTGG GTCCGGTAAC CCCTGATGTC TCCCTGGTGT CCCACAGCGT ATGGAGCAGT 
CCCCCACAGC CCACCCTCTC CCAACTCACC TCGATTGTGC CTCCCAGGCT GACTCATCCA 
TCCCTGGGTA CCAAGGGCAC TGAGCCCCGG GTGTTCCTGG TGAGTTCTGG TACCGTAAGA 
CCCCCCTTCC CCAGGGAGCT GCAGCGGACT GTCCCCGATG GACGTTCACT TGGAGGGGGC 
AGAGAGGGGG ATATACACTG GGACTTTCGT CCACCTTCAC CCAATGGAAC TGAGTCAGAT 
TCGGGACTCT ACCAGGCAGT TGCTGACACC CCGTCTGCCA CGAATGAACC TTCCCCGTCT 
TCCCCTCCTA GTCTGGTTCT TCTGGGGACC GTTGGCTCCC ATGACGAGAC ATTTTACTAC 
CCCCAGACTG AGAGGATAAG GGAGACTTCC ACAGCCCGAC CAGCAGGGAG AGGAGCAGCA 
GGCTCACCCC ACAGTCACTT CGCTGTTGCT GGGGCCAAGC TGGTCACACC AGTCCCTTTC 
CTTTACAAGT TGATTGGCAT CACCCCCAGT AGTGATGAGA GCACTGCCTA TGTGTTGGCC 
AAGAGGTCGC TGGATGCCAG GAATTATGGA ATACTTCAGG ATTGGGTTTC AACAGAGACC 
CCTCATCTAG ACGACACTAG AGAAAAATAC CCAGGCACAG AGAGATATTT ACATGACTGG 
AACCCTCCGA TTGAAAGCAC TACGACCGAA GCATCTCCAC GTTTGAAGAT GACAGTGGAG 
GAGCCAGACA AACTACACAC CACTACAGAG GCACACGACC AAGTCACGAA GACTGAGCCA 
AACCATCCTA GGACATCCAA TAAGGAAGTA TTCAGCACAG ATATCCTGTC AGAGAGGACA 
GCTGCAGACG ATGCTCCTAT TTCAGAAAGG TCCAGTGAAG GCTCAGTGAA GGAATTCCGA 
CACGGGAAAA CTGGTCATTC CGATTTAAGC ATCTCCTACC AGACAGCCTT TATCATTGCA 
GCAGTGTGCA GCGGACCAAT CCTGATCATT ACCCTATTCT GTCTCAGTGA GAAGAAGAGA 
CTCCAAGCCC TTATCCACAG TACCAGCATA ATTGAAAACC AGAGGGCATG CAGCAGCATT 
GAGGACATTG AGCTACAATA TTGTAAAGCC AAGGATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021423073.1
CDS	318..2156
Translation	MSPTLEPGHFGLLVSFQDFLLAVTTDGLLIARHLCFLFLMCFPLAWGGVPGPCRHSVTKGHLLNLNCLIDNQLENGWSITYVFTERQSLSEVCYIKAAFPQILELLNTHFKYVRKSDNGRYVKLLKKVIYDLYSQNCIPEINEEIEDTPLKFVRMHSTSPREALTKARGVIEMYMTLMTKSNGPVDWNCEEEYAEDYQESTTALPTPTAAEPEPERQCTCPTVGPVTPDVSLVSHSVWSSPPQPTLSQLTSIVPPRLTHPSLGTKGTEPRVFLVSSGTVRPPFPRELQRTVPDGRSLGGGREGDIHWDFRPPSPNGTESDSGLYQAVADTPSATNEPSPSSPPSLVLLGTVGSHDETFYYPQTERIRETSTARPAGRGAAGSPHSHFAVAGAKLVTPVPFLYKLIGITPSSDESTAYVLAKRSLDARNYGILQDWVSTETPHLDDTREKYPGTERYLHDWNPPIESTTTEASPRLKMTVEEPDKLHTTTEAHDQVTKTEPNHPRTSNKEVFSTDILSERTAADDAPISERSSEGSVKEFRHGKTGHSDLSISYQTAFIIAAVCSGPILIITLFCLSEKKRLQALIHSTSIIENQRACSSIEDIELQYCKAKD

Target ORF information:

RefSeq Version	XM_021567398.1
Organism	Oncorhynchus mykiss(rainbow trout)
Definition	Oncorhynchus mykiss macrophage colony-stimulating factor precursor (csf1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021567398.1

ORF Insert Sequence:

ATGTCCCCTA CTCTGGAACC TGGGCATTTT GGGTTACTGG TATCATTCCA AGATTTTTTG 
TTAGCGGTAA CAACTGATGG GCTGCTAATA GCTAGGCATC TGTGCTTCCT CTTCCTCATG 
TGCTTCCCTT TGGCGTGGGG TGGCGTCCCT GGTCCATGTC GGCACTCTGT GACCAAGGGT 
CACCTCCTCA ACCTCAACTG CCTGATTGAT AATCAGTTGG AGAATGGCTG GTCAATAACC 
TACGTATTCA CTGAGCGCCA GAGTTTGAGT GAAGTCTGTT ACATCAAAGC AGCATTTCCA 
CAAATCCTGG AGCTTCTGAA CACACACTTC AAGTATGTCA GGAAATCGGA CAACGGGCGC 
TATGTCAAAC TCCTGAAAAA GGTCATATAT GACCTCTACT CCCAAAACTG CATACCAGAA 
ATTAATGAGG AGATTGAGGA CACCCCATTG AAGTTTGTCA GAATGCACAG CACCTCGCCC 
AGAGAGGCTC TGACGAAAGC CCGGGGGGTC ATTGAGATGT ACATGACCCT GATGACCAAG 
AGCAACGGGC CCGTCGACTG GAACTGTGAG GAAGAGTATG CAGAGGATTA CCAAGAATCC 
ACCACTGCCC TCCCCACCCC CACCGCAGCT GAACCTGAAC CTGAACGCCA GTGTACGTGT 
CCGACAGTGG GTCCGGTAAC CCCTGATGTC TCCCTGGTGT CCCACAGCGT ATGGAGCAGT 
CCCCCACAGC CCACCCTCTC CCAACTCACC TCGATTGTGC CTCCCAGGCT GACTCATCCA 
TCCCTGGGTA CCAAGGGCAC TGAGCCCCGG GTGTTCCTGG TGAGTTCTGG TACCGTAAGA 
CCCCCCTTCC CCAGGGAGCT GCAGCGGACT GTCCCCGATG GACGTTCACT TGGAGGGGGC 
AGAGAGGGGG ATATACACTG GGACTTTCGT CCACCTTCAC CCAATGGAAC TGAGTCAGAT 
TCGGGACTCT ACCAGGCAGT TGCTGACACC CCGTCTGCCA CGAATGAACC TTCCCCGTCT 
TCCCCTCCTA GTCTGGTTCT TCTGGGGACC GTTGGCTCCC ATGACGAGAC ATTTTACTAC 
CCCCAGACTG AGAGGATAAG GGAGACTTCC ACAGCCCGAC CAGCAGGGAG AGGAGCAGCA 
GGCTCACCCC ACAGTCACTT CGCTGTTGCT GGGGCCAAGC TGGTCACACC AGTCCCTTTC 
CTTTACAAGT TGATTGGCAT CACCCCCAGT AGTGATGAGA GCACTGCCTA TGTGTTGGCC 
AAGAGGTCGC TGGATGCCAG GAATTATGGA ATACTTCAGG ATTGGGTTTC AACAGAGACC 
CCTCATCTAG ACGACACTAG AGAAAAATAC CCAGGCACAG AGAGATATTT ACATGACTGG 
AACCCTCCGA TTGAAAGCAC TACGACCGAA GCATCTCCAC GTTTGAAGAT GACAGTGGAG 
GAGCCAGACA AACTACACAC CACTACAGAG GCACACGACC AAGTCACGAA GACTGAGCCA 
AACCATCCTA GGACATCCAA TAAGGAAGTA TTCAGCACAG ATATCCTGTC AGAGAGGACA 
GCTGCAGACG ATGCTCCTAT TTCAGAAAGG TCCAGTGAAG GCTCAGTGAA GGAATTCCGA 
CACGGGAAAA CTGGTCATTC CGATTTAAGC ATCTCCTACC AGACAGCCTT TATCATTGCA 
GCAGTGTGCA GCGGACCAAT CCTGATCATT ACCCTATTCT GTCTCAGTGA GAAGAAGAGA 
CTCCAAGCCC TTATCCACAG TACCAGCATA ATTGAAAACC AGAGGGCATG CAGCAGCATT 
GAGGACATTG AGCTACAATA TTGTAAAGCC AAGGATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

csf1 ( XM_021567399.1 ) cDNA ORF clone, Oncorhynchus mykiss(rainbow trout) -> XP_021423074.1 Oncorhynchus mykiss macrophage colony-stimulating factor precursor (csf1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OOf16744
Clone ID Related Accession (Same CDS sequence)	XM_021567399.1
Accession Version	XM_021567399.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1782bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-26
Organism	Oncorhynchus mykiss(rainbow trout)
Product	macrophage colony-stimulating factor precursor isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_035093.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Oncorhynchus mykiss Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACACAC ACAAACCAGC CCACAAAGCC AAGGCTAGGC ATCTGTGCTT CCTCTTCCTC 
ATGTGCTTCC CTTTGGCGTG GGGTGGCGTC CCTGGTCCAT GTCGGCACTC TGTGACCAAG 
GGTCACCTCC TCAACCTCAA CTGCCTGATT GATAATCAGT TGGAGAATGG CTGGTCAATA 
ACCTACGTAT TCACTGAGCG CCAGAGTTTG AGTGAAGTCT GTTACATCAA AGCAGCATTT 
CCACAAATCC TGGAGCTTCT GAACACACAC TTCAAGTATG TCAGGAAATC GGACAACGGG 
CGCTATGTCA AACTCCTGAA AAAGGTCATA TATGACCTCT ACTCCCAAAA CTGCATACCA 
GAAATTAATG AGGAGATTGA GGACACCCCA TTGAAGTTTG TCAGAATGCA CAGCACCTCG 
CCCAGAGAGG CTCTGACGAA AGCCCGGGGG GTCATTGAGA TGTACATGAC CCTGATGACC 
AAGAGCAACG GGCCCGTCGA CTGGAACTGT GAGGAAGAGT ATGCAGAGGA TTACCAAGAA 
TCCACCACTG CCCTCCCCAC CCCCACCGCA GCTGAACCTG AACCTGAACG CCAGTGTACG 
TGTCCGACAG TGGGTCCGGT AACCCCTGAT GTCTCCCTGG TGTCCCACAG CGTATGGAGC 
AGTCCCCCAC AGCCCACCCT CTCCCAACTC ACCTCGATTG TGCCTCCCAG GCTGACTCAT 
CCATCCCTGG GTACCAAGGG CACTGAGCCC CGGGTGTTCC TGGTGAGTTC TGGTACCGTA 
AGACCCCCCT TCCCCAGGGA GCTGCAGCGG ACTGTCCCCG ATGGACGTTC ACTTGGAGGG 
GGCAGAGAGG GGGATATACA CTGGGACTTT CGTCCACCTT CACCCAATGG AACTGAGTCA 
GATTCGGGAC TCTACCAGGC AGTTGCTGAC ACCCCGTCTG CCACGAATGA ACCTTCCCCG 
TCTTCCCCTC CTAGTCTGGT TCTTCTGGGG ACCGTTGGCT CCCATGACGA GACATTTTAC 
TACCCCCAGA CTGAGAGGAT AAGGGAGACT TCCACAGCCC GACCAGCAGG GAGAGGAGCA 
GCAGGCTCAC CCCACAGTCA CTTCGCTGTT GCTGGGGCCA AGCTGGTCAC ACCAGTCCCT 
TTCCTTTACA AGTTGATTGG CATCACCCCC AGTAGTGATG AGAGCACTGC CTATGTGTTG 
GCCAAGAGGT CGCTGGATGC CAGGAATTAT GGAATACTTC AGGATTGGGT TTCAACAGAG 
ACCCCTCATC TAGACGACAC TAGAGAAAAA TACCCAGGCA CAGAGAGATA TTTACATGAC 
TGGAACCCTC CGATTGAAAG CACTACGACC GAAGCATCTC CACGTTTGAA GATGACAGTG 
GAGGAGCCAG ACAAACTACA CACCACTACA GAGGCACACG ACCAAGTCAC GAAGACTGAG 
CCAAACCATC CTAGGACATC CAATAAGGAA GTATTCAGCA CAGATATCCT GTCAGAGAGG 
ACAGCTGCAG ACGATGCTCC TATTTCAGAA AGGTCCAGTG AAGGCTCAGT GAAGGAATTC 
CGACACGGGA AAACTGGTCA TTCCGATTTA AGCATCTCCT ACCAGACAGC CTTTATCATT 
GCAGCAGTGT GCAGCGGACC AATCCTGATC ATTACCCTAT TCTGTCTCAG TGAGAAGAAG 
AGACTCCAAG CCCTTATCCA CAGTACCAGC ATAATTGAAA ACCAGAGGGC ATGCAGCAGC 
ATTGAGGACA TTGAGCTACA ATATTGTAAA GCCAAGGATT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021423074.1
CDS	104..1885
Translation	MNTHKPAHKAKARHLCFLFLMCFPLAWGGVPGPCRHSVTKGHLLNLNCLIDNQLENGWSITYVFTERQSLSEVCYIKAAFPQILELLNTHFKYVRKSDNGRYVKLLKKVIYDLYSQNCIPEINEEIEDTPLKFVRMHSTSPREALTKARGVIEMYMTLMTKSNGPVDWNCEEEYAEDYQESTTALPTPTAAEPEPERQCTCPTVGPVTPDVSLVSHSVWSSPPQPTLSQLTSIVPPRLTHPSLGTKGTEPRVFLVSSGTVRPPFPRELQRTVPDGRSLGGGREGDIHWDFRPPSPNGTESDSGLYQAVADTPSATNEPSPSSPPSLVLLGTVGSHDETFYYPQTERIRETSTARPAGRGAAGSPHSHFAVAGAKLVTPVPFLYKLIGITPSSDESTAYVLAKRSLDARNYGILQDWVSTETPHLDDTREKYPGTERYLHDWNPPIESTTTEASPRLKMTVEEPDKLHTTTEAHDQVTKTEPNHPRTSNKEVFSTDILSERTAADDAPISERSSEGSVKEFRHGKTGHSDLSISYQTAFIIAAVCSGPILIITLFCLSEKKRLQALIHSTSIIENQRACSSIEDIELQYCKAKD

Target ORF information:

RefSeq Version	XM_021567399.1
Organism	Oncorhynchus mykiss(rainbow trout)
Definition	Oncorhynchus mykiss macrophage colony-stimulating factor precursor (csf1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021567399.1

ORF Insert Sequence:

ATGAACACAC ACAAACCAGC CCACAAAGCC AAGGCTAGGC ATCTGTGCTT CCTCTTCCTC 
ATGTGCTTCC CTTTGGCGTG GGGTGGCGTC CCTGGTCCAT GTCGGCACTC TGTGACCAAG 
GGTCACCTCC TCAACCTCAA CTGCCTGATT GATAATCAGT TGGAGAATGG CTGGTCAATA 
ACCTACGTAT TCACTGAGCG CCAGAGTTTG AGTGAAGTCT GTTACATCAA AGCAGCATTT 
CCACAAATCC TGGAGCTTCT GAACACACAC TTCAAGTATG TCAGGAAATC GGACAACGGG 
CGCTATGTCA AACTCCTGAA AAAGGTCATA TATGACCTCT ACTCCCAAAA CTGCATACCA 
GAAATTAATG AGGAGATTGA GGACACCCCA TTGAAGTTTG TCAGAATGCA CAGCACCTCG 
CCCAGAGAGG CTCTGACGAA AGCCCGGGGG GTCATTGAGA TGTACATGAC CCTGATGACC 
AAGAGCAACG GGCCCGTCGA CTGGAACTGT GAGGAAGAGT ATGCAGAGGA TTACCAAGAA 
TCCACCACTG CCCTCCCCAC CCCCACCGCA GCTGAACCTG AACCTGAACG CCAGTGTACG 
TGTCCGACAG TGGGTCCGGT AACCCCTGAT GTCTCCCTGG TGTCCCACAG CGTATGGAGC 
AGTCCCCCAC AGCCCACCCT CTCCCAACTC ACCTCGATTG TGCCTCCCAG GCTGACTCAT 
CCATCCCTGG GTACCAAGGG CACTGAGCCC CGGGTGTTCC TGGTGAGTTC TGGTACCGTA 
AGACCCCCCT TCCCCAGGGA GCTGCAGCGG ACTGTCCCCG ATGGACGTTC ACTTGGAGGG 
GGCAGAGAGG GGGATATACA CTGGGACTTT CGTCCACCTT CACCCAATGG AACTGAGTCA 
GATTCGGGAC TCTACCAGGC AGTTGCTGAC ACCCCGTCTG CCACGAATGA ACCTTCCCCG 
TCTTCCCCTC CTAGTCTGGT TCTTCTGGGG ACCGTTGGCT CCCATGACGA GACATTTTAC 
TACCCCCAGA CTGAGAGGAT AAGGGAGACT TCCACAGCCC GACCAGCAGG GAGAGGAGCA 
GCAGGCTCAC CCCACAGTCA CTTCGCTGTT GCTGGGGCCA AGCTGGTCAC ACCAGTCCCT 
TTCCTTTACA AGTTGATTGG CATCACCCCC AGTAGTGATG AGAGCACTGC CTATGTGTTG 
GCCAAGAGGT CGCTGGATGC CAGGAATTAT GGAATACTTC AGGATTGGGT TTCAACAGAG 
ACCCCTCATC TAGACGACAC TAGAGAAAAA TACCCAGGCA CAGAGAGATA TTTACATGAC 
TGGAACCCTC CGATTGAAAG CACTACGACC GAAGCATCTC CACGTTTGAA GATGACAGTG 
GAGGAGCCAG ACAAACTACA CACCACTACA GAGGCACACG ACCAAGTCAC GAAGACTGAG 
CCAAACCATC CTAGGACATC CAATAAGGAA GTATTCAGCA CAGATATCCT GTCAGAGAGG 
ACAGCTGCAG ACGATGCTCC TATTTCAGAA AGGTCCAGTG AAGGCTCAGT GAAGGAATTC 
CGACACGGGA AAACTGGTCA TTCCGATTTA AGCATCTCCT ACCAGACAGC CTTTATCATT 
GCAGCAGTGT GCAGCGGACC AATCCTGATC ATTACCCTAT TCTGTCTCAG TGAGAAGAAG 
AGACTCCAAG CCCTTATCCA CAGTACCAGC ATAATTGAAA ACCAGAGGGC ATGCAGCAGC 
ATTGAGGACA TTGAGCTACA ATATTGTAAA GCCAAGGATT GA