apoe cDNA ORF clone, Oncorhynchus mykiss(rainbow trout)

Gene
Clones
gRNAs

Summary

Gene Symbol	apoe
Entrez Gene ID	100136023
Full Name	apolipoprotein E
Gene Type	protein-coding
Organism	Oncorhynchus mykiss(rainbow trout)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001124346.1	NP_001117818.1	apolipoprotein E precursor

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Conserved protein motifs and structural organization of a fish gene homologous to mammalian apolipoprotein E.
Durliat M, Andr? M, Babin PJ
European journal of biochemistry267(2)549-59(2000 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following apoe gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the apoe cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OOf00462	NM_001124346.1 Latest version!	Oncorhynchus mykiss apolipoprotein E (apoe), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

apoe ( NM_001124346.1 ) cDNA ORF clone, Oncorhynchus mykiss(rainbow trout) -> NP_001117818.1 Oncorhynchus mykiss apolipoprotein E (apoe), mRNA.

Price & Availability↑

CloneID	OOf00462
Clone ID Related Accession (Same CDS sequence)	NM_001124346.1
Accession Version	NM_001124346.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 828bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-07-02
Organism	Oncorhynchus mykiss(rainbow trout)
Product	apolipoprotein E precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AJ132620.1. ##Evidence-Data-START## Transcript exon combination :: AJ132620.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00041048, SAMD00041049 [ECO:0000350] ##Evidence-Data-END##

ATGAAGGCTG TGGCAATAAT CCTTGCTCTG GCAGTCATCT CTGGCTGCCA TGCCCGGGCT 
GTGCGCCAGG CAGAGGCCCT CCCGAACCTC TGGGAGGAGA ACGTTGAGCG CTTCTGGACC 
TATGTGTCTG AGATCAACAG CAGAGCCGAC GGATTGGTGG AAAACCTCAA GGCCTCCCAG 
CTCAGCAGAG AACTTGACAC CCTGATTACT GACACCATGG CTGAGCTGAC CGTGTACAGG 
GAGGACATCC AGACCAAGTT GGTGCCCTAC GCCCAGGACA CAGCCGCCCT GCTGGGCCAG 
GACTTGCAGC TTCTGACCAC CAAGCTCCAG ACCGACATGG TTGACGCCAA GGAGCGCAGC 
ACACAGTACC TGGGAGAGCT CAAGACTATG ATGGAGCAGA ATGCTGATGA TGTCCACAAC 
CGCGTCAACA CCTACACCCG CAAACTGAAG AAACGCCTTA ACAAGGACAC AGAGGAGATC 
CGCAACACAG TGGCTACCTA CCTGGGAGAA ATCCAGTCCC GTACCTCCCA GAACATGGAT 
CTCGTGAAGG AGCGTGTGGA GCCCTTCGTG AGTCAGGCCC ACGACACCGC CGCCCAGAGG 
CTGGGCAGCT TCACTGACCT TCTGAAGAAC CAGGCCCAGG ACCTGAGCCA GCAGGTCTCC 
ACCCAGGCCG AAGGCATGCG CGAGCAGCTG GAGGCCACCG CCGAGGACCT GCGCACCACC 
CTGGAGGGCA AGATCGATGA GCTCAGCAGC CTGATCGCCC CCTACGCCGC CAAGATCCGT 
GAGCACCTCC AGACCGCCAT GGACAAGGTC AAGGAGACCA CCGTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001117818.1
CDS	48..875
Translation	MKAVAIILALAVISGCHARAVRQAEALPNLWEENVERFWTYVSEINSRADGLVENLKASQLSRELDTLITDTMAELTVYREDIQTKLVPYAQDTAALLGQDLQLLTTKLQTDMVDAKERSTQYLGELKTMMEQNADDVHNRVNTYTRKLKKRLNKDTEEIRNTVATYLGEIQSRTSQNMDLVKERVEPFVSQAHDTAAQRLGSFTDLLKNQAQDLSQQVSTQAEGMREQLEATAEDLRTTLEGKIDELSSLIAPYAAKIREHLQTAMDKVKETTV

Target ORF information:

RefSeq Version	NM_001124346.1
Organism	Oncorhynchus mykiss(rainbow trout)
Definition	Oncorhynchus mykiss apolipoprotein E (apoe), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001124346.1

ORF Insert Sequence:

ATGAAGGCTG TGGCAATAAT CCTTGCTCTG GCAGTCATCT CTGGCTGCCA TGCCCGGGCT 
GTGCGCCAGG CAGAGGCCCT CCCGAACCTC TGGGAGGAGA ACGTTGAGCG CTTCTGGACC 
TATGTGTCTG AGATCAACAG CAGAGCCGAC GGATTGGTGG AAAACCTCAA GGCCTCCCAG 
CTCAGCAGAG AACTTGACAC CCTGATTACT GACACCATGG CTGAGCTGAC CGTGTACAGG 
GAGGACATCC AGACCAAGTT GGTGCCCTAC GCCCAGGACA CAGCCGCCCT GCTGGGCCAG 
GACTTGCAGC TTCTGACCAC CAAGCTCCAG ACCGACATGG TTGACGCCAA GGAGCGCAGC 
ACACAGTACC TGGGAGAGCT CAAGACTATG ATGGAGCAGA ATGCTGATGA TGTCCACAAC 
CGCGTCAACA CCTACACCCG CAAACTGAAG AAACGCCTTA ACAAGGACAC AGAGGAGATC 
CGCAACACAG TGGCTACCTA CCTGGGAGAA ATCCAGTCCC GTACCTCCCA GAACATGGAT 
CTCGTGAAGG AGCGTGTGGA GCCCTTCGTG AGTCAGGCCC ACGACACCGC CGCCCAGAGG 
CTGGGCAGCT TCACTGACCT TCTGAAGAAC CAGGCCCAGG ACCTGAGCCA GCAGGTCTCC 
ACCCAGGCCG AAGGCATGCG CGAGCAGCTG GAGGCCACCG CCGAGGACCT GCGCACCACC 
CTGGAGGGCA AGATCGATGA GCTCAGCAGC CTGATCGCCC CCTACGCCGC CAAGATCCGT 
GAGCACCTCC AGACCGCCAT GGACAAGGTC AAGGAGACCA CCGTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Conserved protein motifs and structural organization of a fish gene homologous to mammalian apolipoprotein E.
European journal of biochemistry267(2)549-59(2000 Jan)
Durliat M,Andr? M,Babin PJ