LOC109868058 cDNA ORF clone, Oncorhynchus kisutch(coho salmon)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC109868058
Entrez Gene ID	109868058
Full Name	thrombospondin-4-B-like
Gene Type	protein-coding
Organism	Oncorhynchus kisutch(coho salmon)

mRNA and Protein(s)

mRNA	Protein	Name
XM_020457463.1	XP_020313052.1	thrombospondin-4-B-like
XM_020457463.2	XP_020313052.1	thrombospondin-4-B-like

Pathways from BioSystems
Gene Ontology

The following LOC109868058 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC109868058 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OOn40599	XM_020457463.2 Latest version!	Oncorhynchus kisutch thrombospondin-4-B-like (LOC109868058), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
View Old Accession Versions >>
OOn40599	XM_020457463.1	Oncorhynchus kisutch thrombospondin-4-B-like (LOC109868058), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$510.30 $729.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC109868058 ( XM_020457463.2 ) cDNA ORF clone, Oncorhynchus kisutch(coho salmon) -> XP_020313052.1 Oncorhynchus kisutch thrombospondin-4-B-like (LOC109868058), mRNA.

Price & Availability↑

CloneID	OOn40599
Clone ID Related Accession (Same CDS sequence)	XM_020457463.1 , XM_020457463.2
Accession Version	XM_020457463.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2814bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-08
Organism	Oncorhynchus kisutch(coho salmon)
Product	thrombospondin-4-B-like
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_034196.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 9, 2019 this sequence version replaced XM_020457463.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Oncorhynchus kisutch Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGCTGT GGACGATGCT GATCGTGGCT TCGCAGCTGA TCTTAAACCT GTGCTCAGAC 
GGAGCCCAGG GATCAGTCTA TGATCTCCTA AACTCCCCGG ACTGTCTGCC AGACCTGCTG 
CAGGGCGGCC TGGTAGAGCA GGGGGTGAAT GAGGCGTTCA TCCTCACCAC CTTTAAACTG 
CAGCCCAAGA CTGGCACCAC CGTGTTTGGT CTGTACAACC CCCGGGACAA CAGCAAGTAC 
TTTGAGTTCA CCGTGCTGGG GAAACTCAAC AGAGCTGTGC TGCGTTACCT GAGGAGTGAT 
CGGAGGATGA GCTCTGTGAC CTTCAACAAC ATCCAACTGG CGGACGGTCA GCGCCACCGC 
CTCCTCTTCC ACCTGAAGGG GATGCAGCAG GGCCCAGGGG CGGTGGAGCT GCACCTGGAC 
TGCAGACTGG TGGAGACGGT CAGGGATCTC CCCTCCGTGT TTCAGGGCCT GCCTGCAGGC 
TACGGCGTGG TGGAGCTGAA GAGCATGCAG GGCAAAGCAG AGGAGGAGCT GGAGGAATTG 
AAACTGGTGG TGGGAGACAC GTTCGAGAAC GTAGCCTCTC TACAAAACTG CCACCAACAA 
GGAGACTCGG TGCAGACTCT GGGGGTCAAC ACAAAGCAAC TCTCCAATCA AATGCTTGAG 
CTCACCCAGG TGATAAATGA GCTGAAAGAT GTCCTTATTC AGCAGGTCAA AGAGACATCT 
TTTCTCAGAA ACACCATCTC TGAGTGCCAG GCTTGTGGAC TGGGTGGAAG CGAGGTCATT 
GAGCCAAAGT GTGCCCCTGG TGTTTGTTTC CGTGACGACA TGTGTATTGA GACGGAGGAG 
GGTGTTGAGT GTGGACCCTG TCCTGATGGA TACACAGGGG ATGGCTTCAG CTGTGATGAT 
GTGGATGAGT GTCAGTTTAA CCCTTGCTTC GCCGGGGTAA AGTGTGTGAA CACAGCTCCA 
GGGTTTCGCT GTGACGCATG TCCTCTGGGC TACACTGGTC TGGCTGTGGA GGGGGTGGGA 
GTGGTCTACG CACAGACAAA CAAACAGGTC TGTGACGACA TTGATGAGTG CAAAGGACCC 
AACAACGGAG GGTGCACTGC TGACTCTCTC TGTCACAACT CTGCGGGTTC ATACGTCTGT 
GGGGGCTGTA AAACAGGCTA CATAGGGGGC CAGGTGAAGG GCTGTAAGCC AGAGAGGAGC 
TGTGGTAACA GTCGGACCAA CCCCTGTGAT GTCAACGCCC AGTGTATCCA AGAAAGAGAC 
GGCTCCATCA CCTGTCAGTG TGGGATTGGC TGGGCTGGTA ATGGCTACCT ATGTGGGAAG 
GACACAGACA TTGATGGATA CCCTGATGAG AAACTCAAGT GCAAGGATAT GGTCTGTAAA 
AAGGATAACT GCATGCGTGT TCCCAACTCT GGCCAAGAAG ATGCTGACGA GGACGGACAA 
GGGGACGCTT GTGACGATGA CGCTGATGGA GACGGTATTC TGAATGAACA GGATAACTGC 
TGGCTGAAGC CTAACGTGGA CCAGAGGAAT AGTGACAAGG ACAACCACGG TGATGCCTGT 
GACAACTGTC GTACGGTGGA GAACCCCGAT CAGAGGGACA CTGACGGTGA CAGGAAAGGA 
GACGCCTGCG ATGATGACAT GGATGGAGAC GGCTTGAAGA ACTTCCTGGA TAACTGCCAG 
ATGGTGGTGA ACCGGGACCA GTTGGACCGG GATGGAGACG GAGTGGGAGA CGCCTGTGAC 
AGCTGCCCCG ATATTCCTAA TCCTAACCAG TCCGATATCG ATGATGACCT GGTTGGGGAT 
TCTTGTGACA CAAACCAAGA CAGTGATGGT GACGGTCACC AGGACACCAA GGACAACTGT 
CCCAACGTGA TCAACAGCTC CCAGCTGGAC ACAGACAAAG ACGGCCTGGG GGATGAGTGT 
GACGATGACG ATGACAACGA CAGCATCCCT GACTTCCTGC CACCAGGACC CGACAACTGT 
AGACTGGTGC CCAACCCCGA CCAGCTGGAT GAGAACAATG ACGGTGTTGG GGATGTGTGT 
GAGTCAGACT TTGACCAGGA CAAGGTGATT GACAGAATAG ACAACTGTCC AGAGAACGCT 
GAGGTCACTC TGACAGACTT CAGAGCCTAT CAGACGGTGG TGCTGGACCC CGAGGGTGAC 
GCCCAGATTG ACCCCAACTG GGTTGTCCTC AATCAGGGAA TGGAGATTGT GCAGACCATG 
AACAGTGACC CTGGACTGGC TGTGGGTTAC ACTGCGTTCA GTGGGGTGGA CTTTGAGGGG 
ACGTTCCATG TAAACACAGT TACGGACGAT GACTACGCAG GCTTCATCTT TGGCTACCAG 
GACTCCTCCT CCTTCTACGT GGTCATGTGG AAACAGACTG AGCAGACCTA CTGGCAGGCC 
ACACCCTTCA GAGCAGTGGC CGAGCCTGGC ATCCAGCTCA AGGCTGTGAA GTCTAAGTCG 
GGTCCAGGTG AGCACCTGAG GAACTCCCTT TGGCACACAG GTGACACCAA CGACCAGGTG 
CGTCTGTTGT GGAAGGACCC CAGGAACGTG GGCTGGAAGG ACAAGGTTTC CTACCGCTGG 
TACCTGCAGC ACCGGCCCCA GGTTGGATAC ATCAGGGCCC GTTTCTATGA AGGTACAGAA 
CTGGTGGCTG ACTCTGGTGT GACCATTGAC ACCACCATGA GAGGAGGGCG ACTGGGCGTT 
TTCTGTTTCT CTCAGGAAAA CATAATCTGG TCCAACCTAA AGTATCGCTG CAATGACACC 
ATCCCTGAGG ACTTCCAGTT CAGCACGCAG CACGGCGTCA ACGACAGTCT CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_020313052.1
CDS	201..3014
Translation	MGLWTMLIVASQLILNLCSDGAQGSVYDLLNSPDCLPDLLQGGLVEQGVNEAFILTTFKLQPKTGTTVFGLYNPRDNSKYFEFTVLGKLNRAVLRYLRSDRRMSSVTFNNIQLADGQRHRLLFHLKGMQQGPGAVELHLDCRLVETVRDLPSVFQGLPAGYGVVELKSMQGKAEEELEELKLVVGDTFENVASLQNCHQQGDSVQTLGVNTKQLSNQMLELTQVINELKDVLIQQVKETSFLRNTISECQACGLGGSEVIEPKCAPGVCFRDDMCIETEEGVECGPCPDGYTGDGFSCDDVDECQFNPCFAGVKCVNTAPGFRCDACPLGYTGLAVEGVGVVYAQTNKQVCDDIDECKGPNNGGCTADSLCHNSAGSYVCGGCKTGYIGGQVKGCKPERSCGNSRTNPCDVNAQCIQERDGSITCQCGIGWAGNGYLCGKDTDIDGYPDEKLKCKDMVCKKDNCMRVPNSGQEDADEDGQGDACDDDADGDGILNEQDNCWLKPNVDQRNSDKDNHGDACDNCRTVENPDQRDTDGDRKGDACDDDMDGDGLKNFLDNCQMVVNRDQLDRDGDGVGDACDSCPDIPNPNQSDIDDDLVGDSCDTNQDSDGDGHQDTKDNCPNVINSSQLDTDKDGLGDECDDDDDNDSIPDFLPPGPDNCRLVPNPDQLDENNDGVGDVCESDFDQDKVIDRIDNCPENAEVTLTDFRAYQTVVLDPEGDAQIDPNWVVLNQGMEIVQTMNSDPGLAVGYTAFSGVDFEGTFHVNTVTDDDYAGFIFGYQDSSSFYVVMWKQTEQTYWQATPFRAVAEPGIQLKAVKSKSGPGEHLRNSLWHTGDTNDQVRLLWKDPRNVGWKDKVSYRWYLQHRPQVGYIRARFYEGTELVADSGVTIDTTMRGGRLGVFCFSQENIIWSNLKYRCNDTIPEDFQFSTQHGVNDSL

Target ORF information:

RefSeq Version	XM_020457463.2
Organism	Oncorhynchus kisutch(coho salmon)
Definition	Oncorhynchus kisutch thrombospondin-4-B-like (LOC109868058), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_020457463.2

ORF Insert Sequence:

ATGGGGCTGT GGACGATGCT GATCGTGGCT TCGCAGCTGA TCTTAAACCT GTGCTCAGAC 
GGAGCCCAGG GATCAGTCTA TGATCTCCTA AACTCCCCGG ACTGTCTGCC AGACCTGCTG 
CAGGGCGGCC TGGTAGAGCA GGGGGTGAAT GAGGCGTTCA TCCTCACCAC CTTTAAACTG 
CAGCCCAAGA CTGGCACCAC CGTGTTTGGT CTGTACAACC CCCGGGACAA CAGCAAGTAC 
TTTGAGTTCA CCGTGCTGGG GAAACTCAAC AGAGCTGTGC TGCGTTACCT GAGGAGTGAT 
CGGAGGATGA GCTCTGTGAC CTTCAACAAC ATCCAACTGG CGGACGGTCA GCGCCACCGC 
CTCCTCTTCC ACCTGAAGGG GATGCAGCAG GGCCCAGGGG CGGTGGAGCT GCACCTGGAC 
TGCAGACTGG TGGAGACGGT CAGGGATCTC CCCTCCGTGT TTCAGGGCCT GCCTGCAGGC 
TACGGCGTGG TGGAGCTGAA GAGCATGCAG GGCAAAGCAG AGGAGGAGCT GGAGGAATTG 
AAACTGGTGG TGGGAGACAC GTTCGAGAAC GTAGCCTCTC TACAAAACTG CCACCAACAA 
GGAGACTCGG TGCAGACTCT GGGGGTCAAC ACAAAGCAAC TCTCCAATCA AATGCTTGAG 
CTCACCCAGG TGATAAATGA GCTGAAAGAT GTCCTTATTC AGCAGGTCAA AGAGACATCT 
TTTCTCAGAA ACACCATCTC TGAGTGCCAG GCTTGTGGAC TGGGTGGAAG CGAGGTCATT 
GAGCCAAAGT GTGCCCCTGG TGTTTGTTTC CGTGACGACA TGTGTATTGA GACGGAGGAG 
GGTGTTGAGT GTGGACCCTG TCCTGATGGA TACACAGGGG ATGGCTTCAG CTGTGATGAT 
GTGGATGAGT GTCAGTTTAA CCCTTGCTTC GCCGGGGTAA AGTGTGTGAA CACAGCTCCA 
GGGTTTCGCT GTGACGCATG TCCTCTGGGC TACACTGGTC TGGCTGTGGA GGGGGTGGGA 
GTGGTCTACG CACAGACAAA CAAACAGGTC TGTGACGACA TTGATGAGTG CAAAGGACCC 
AACAACGGAG GGTGCACTGC TGACTCTCTC TGTCACAACT CTGCGGGTTC ATACGTCTGT 
GGGGGCTGTA AAACAGGCTA CATAGGGGGC CAGGTGAAGG GCTGTAAGCC AGAGAGGAGC 
TGTGGTAACA GTCGGACCAA CCCCTGTGAT GTCAACGCCC AGTGTATCCA AGAAAGAGAC 
GGCTCCATCA CCTGTCAGTG TGGGATTGGC TGGGCTGGTA ATGGCTACCT ATGTGGGAAG 
GACACAGACA TTGATGGATA CCCTGATGAG AAACTCAAGT GCAAGGATAT GGTCTGTAAA 
AAGGATAACT GCATGCGTGT TCCCAACTCT GGCCAAGAAG ATGCTGACGA GGACGGACAA 
GGGGACGCTT GTGACGATGA CGCTGATGGA GACGGTATTC TGAATGAACA GGATAACTGC 
TGGCTGAAGC CTAACGTGGA CCAGAGGAAT AGTGACAAGG ACAACCACGG TGATGCCTGT 
GACAACTGTC GTACGGTGGA GAACCCCGAT CAGAGGGACA CTGACGGTGA CAGGAAAGGA 
GACGCCTGCG ATGATGACAT GGATGGAGAC GGCTTGAAGA ACTTCCTGGA TAACTGCCAG 
ATGGTGGTGA ACCGGGACCA GTTGGACCGG GATGGAGACG GAGTGGGAGA CGCCTGTGAC 
AGCTGCCCCG ATATTCCTAA TCCTAACCAG TCCGATATCG ATGATGACCT GGTTGGGGAT 
TCTTGTGACA CAAACCAAGA CAGTGATGGT GACGGTCACC AGGACACCAA GGACAACTGT 
CCCAACGTGA TCAACAGCTC CCAGCTGGAC ACAGACAAAG ACGGCCTGGG GGATGAGTGT 
GACGATGACG ATGACAACGA CAGCATCCCT GACTTCCTGC CACCAGGACC CGACAACTGT 
AGACTGGTGC CCAACCCCGA CCAGCTGGAT GAGAACAATG ACGGTGTTGG GGATGTGTGT 
GAGTCAGACT TTGACCAGGA CAAGGTGATT GACAGAATAG ACAACTGTCC AGAGAACGCT 
GAGGTCACTC TGACAGACTT CAGAGCCTAT CAGACGGTGG TGCTGGACCC CGAGGGTGAC 
GCCCAGATTG ACCCCAACTG GGTTGTCCTC AATCAGGGAA TGGAGATTGT GCAGACCATG 
AACAGTGACC CTGGACTGGC TGTGGGTTAC ACTGCGTTCA GTGGGGTGGA CTTTGAGGGG 
ACGTTCCATG TAAACACAGT TACGGACGAT GACTACGCAG GCTTCATCTT TGGCTACCAG 
GACTCCTCCT CCTTCTACGT GGTCATGTGG AAACAGACTG AGCAGACCTA CTGGCAGGCC 
ACACCCTTCA GAGCAGTGGC CGAGCCTGGC ATCCAGCTCA AGGCTGTGAA GTCTAAGTCG 
GGTCCAGGTG AGCACCTGAG GAACTCCCTT TGGCACACAG GTGACACCAA CGACCAGGTG 
CGTCTGTTGT GGAAGGACCC CAGGAACGTG GGCTGGAAGG ACAAGGTTTC CTACCGCTGG 
TACCTGCAGC ACCGGCCCCA GGTTGGATAC ATCAGGGCCC GTTTCTATGA AGGTACAGAA 
CTGGTGGCTG ACTCTGGTGT GACCATTGAC ACCACCATGA GAGGAGGGCG ACTGGGCGTT 
TTCTGTTTCT CTCAGGAAAA CATAATCTGG TCCAACCTAA AGTATCGCTG CAATGACACC 
ATCCCTGAGG ACTTCCAGTT CAGCACGCAG CACGGCGTCA ACGACAGTCT CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC109868058 ( XM_020457463.1 ) cDNA ORF clone, Oncorhynchus kisutch(coho salmon) -> XP_020313052.1 Oncorhynchus kisutch thrombospondin-4-B-like (LOC109868058), mRNA.

Price & Availability↑

CloneID	OOn40599
Clone ID Related Accession (Same CDS sequence)	XM_020457463.1 , XM_020457463.2
Accession Version	XM_020457463.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2814bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-03-12
Organism	Oncorhynchus kisutch(coho salmon)
Product	thrombospondin-4-B-like
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_034196.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Oncorhynchus kisutch Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGCTGT GGACGATGCT GATCGTGGCT TCGCAGCTGA TCTTAAACCT GTGCTCAGAC 
GGAGCCCAGG GATCAGTCTA TGATCTCCTA AACTCCCCGG ACTGTCTGCC AGACCTGCTG 
CAGGGCGGCC TGGTAGAGCA GGGGGTGAAT GAGGCGTTCA TCCTCACCAC CTTTAAACTG 
CAGCCCAAGA CTGGCACCAC CGTGTTTGGT CTGTACAACC CCCGGGACAA CAGCAAGTAC 
TTTGAGTTCA CCGTGCTGGG GAAACTCAAC AGAGCTGTGC TGCGTTACCT GAGGAGTGAT 
CGGAGGATGA GCTCTGTGAC CTTCAACAAC ATCCAACTGG CGGACGGTCA GCGCCACCGC 
CTCCTCTTCC ACCTGAAGGG GATGCAGCAG GGCCCAGGGG CGGTGGAGCT GCACCTGGAC 
TGCAGACTGG TGGAGACGGT CAGGGATCTC CCCTCCGTGT TTCAGGGCCT GCCTGCAGGC 
TACGGCGTGG TGGAGCTGAA GAGCATGCAG GGCAAAGCAG AGGAGGAGCT GGAGGAATTG 
AAACTGGTGG TGGGAGACAC GTTCGAGAAC GTAGCCTCTC TACAAAACTG CCACCAACAA 
GGAGACTCGG TGCAGACTCT GGGGGTCAAC ACAAAGCAAC TCTCCAATCA AATGCTTGAG 
CTCACCCAGG TGATAAATGA GCTGAAAGAT GTCCTTATTC AGCAGGTCAA AGAGACATCT 
TTTCTCAGAA ACACCATCTC TGAGTGCCAG GCTTGTGGAC TGGGTGGAAG CGAGGTCATT 
GAGCCAAAGT GTGCCCCTGG TGTTTGTTTC CGTGACGACA TGTGTATTGA GACGGAGGAG 
GGTGTTGAGT GTGGACCCTG TCCTGATGGA TACACAGGGG ATGGCTTCAG CTGTGATGAT 
GTGGATGAGT GTCAGTTTAA CCCTTGCTTC GCCGGGGTAA AGTGTGTGAA CACAGCTCCA 
GGGTTTCGCT GTGACGCATG TCCTCTGGGC TACACTGGTC TGGCTGTGGA GGGGGTGGGA 
GTGGTCTACG CACAGACAAA CAAACAGGTC TGTGACGACA TTGATGAGTG CAAAGGACCC 
AACAACGGAG GGTGCACTGC TGACTCTCTC TGTCACAACT CTGCGGGTTC ATACGTCTGT 
GGGGGCTGTA AAACAGGCTA CATAGGGGGC CAGGTGAAGG GCTGTAAGCC AGAGAGGAGC 
TGTGGTAACA GTCGGACCAA CCCCTGTGAT GTCAACGCCC AGTGTATCCA AGAAAGAGAC 
GGCTCCATCA CCTGTCAGTG TGGGATTGGC TGGGCTGGTA ATGGCTACCT ATGTGGGAAG 
GACACAGACA TTGATGGATA CCCTGATGAG AAACTCAAGT GCAAGGATAT GGTCTGTAAA 
AAGGATAACT GCATGCGTGT TCCCAACTCT GGCCAAGAAG ATGCTGACGA GGACGGACAA 
GGGGACGCTT GTGACGATGA CGCTGATGGA GACGGTATTC TGAATGAACA GGATAACTGC 
TGGCTGAAGC CTAACGTGGA CCAGAGGAAT AGTGACAAGG ACAACCACGG TGATGCCTGT 
GACAACTGTC GTACGGTGGA GAACCCCGAT CAGAGGGACA CTGACGGTGA CAGGAAAGGA 
GACGCCTGCG ATGATGACAT GGATGGAGAC GGCTTGAAGA ACTTCCTGGA TAACTGCCAG 
ATGGTGGTGA ACCGGGACCA GTTGGACCGG GATGGAGACG GAGTGGGAGA CGCCTGTGAC 
AGCTGCCCCG ATATTCCTAA TCCTAACCAG TCCGATATCG ATGATGACCT GGTTGGGGAT 
TCTTGTGACA CAAACCAAGA CAGTGATGGT GACGGTCACC AGGACACCAA GGACAACTGT 
CCCAACGTGA TCAACAGCTC CCAGCTGGAC ACAGACAAAG ACGGCCTGGG GGATGAGTGT 
GACGATGACG ATGACAACGA CAGCATCCCT GACTTCCTGC CACCAGGACC CGACAACTGT 
AGACTGGTGC CCAACCCCGA CCAGCTGGAT GAGAACAATG ACGGTGTTGG GGATGTGTGT 
GAGTCAGACT TTGACCAGGA CAAGGTGATT GACAGAATAG ACAACTGTCC AGAGAACGCT 
GAGGTCACTC TGACAGACTT CAGAGCCTAT CAGACGGTGG TGCTGGACCC CGAGGGTGAC 
GCCCAGATTG ACCCCAACTG GGTTGTCCTC AATCAGGGAA TGGAGATTGT GCAGACCATG 
AACAGTGACC CTGGACTGGC TGTGGGTTAC ACTGCGTTCA GTGGGGTGGA CTTTGAGGGG 
ACGTTCCATG TAAACACAGT TACGGACGAT GACTACGCAG GCTTCATCTT TGGCTACCAG 
GACTCCTCCT CCTTCTACGT GGTCATGTGG AAACAGACTG AGCAGACCTA CTGGCAGGCC 
ACACCCTTCA GAGCAGTGGC CGAGCCTGGC ATCCAGCTCA AGGCTGTGAA GTCTAAGTCG 
GGTCCAGGTG AGCACCTGAG GAACTCCCTT TGGCACACAG GTGACACCAA CGACCAGGTG 
CGTCTGTTGT GGAAGGACCC CAGGAACGTG GGCTGGAAGG ACAAGGTTTC CTACCGCTGG 
TACCTGCAGC ACCGGCCCCA GGTTGGATAC ATCAGGGCCC GTTTCTATGA AGGTACAGAA 
CTGGTGGCTG ACTCTGGTGT GACCATTGAC ACCACCATGA GAGGAGGGCG ACTGGGCGTT 
TTCTGTTTCT CTCAGGAAAA CATAATCTGG TCCAACCTAA AGTATCGCTG CAATGACACC 
ATCCCTGAGG ACTTCCAGTT CAGCACGCAG CACGGCGTCA ACGACAGTCT CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_020313052.1
CDS	175..2988
Translation	MGLWTMLIVASQLILNLCSDGAQGSVYDLLNSPDCLPDLLQGGLVEQGVNEAFILTTFKLQPKTGTTVFGLYNPRDNSKYFEFTVLGKLNRAVLRYLRSDRRMSSVTFNNIQLADGQRHRLLFHLKGMQQGPGAVELHLDCRLVETVRDLPSVFQGLPAGYGVVELKSMQGKAEEELEELKLVVGDTFENVASLQNCHQQGDSVQTLGVNTKQLSNQMLELTQVINELKDVLIQQVKETSFLRNTISECQACGLGGSEVIEPKCAPGVCFRDDMCIETEEGVECGPCPDGYTGDGFSCDDVDECQFNPCFAGVKCVNTAPGFRCDACPLGYTGLAVEGVGVVYAQTNKQVCDDIDECKGPNNGGCTADSLCHNSAGSYVCGGCKTGYIGGQVKGCKPERSCGNSRTNPCDVNAQCIQERDGSITCQCGIGWAGNGYLCGKDTDIDGYPDEKLKCKDMVCKKDNCMRVPNSGQEDADEDGQGDACDDDADGDGILNEQDNCWLKPNVDQRNSDKDNHGDACDNCRTVENPDQRDTDGDRKGDACDDDMDGDGLKNFLDNCQMVVNRDQLDRDGDGVGDACDSCPDIPNPNQSDIDDDLVGDSCDTNQDSDGDGHQDTKDNCPNVINSSQLDTDKDGLGDECDDDDDNDSIPDFLPPGPDNCRLVPNPDQLDENNDGVGDVCESDFDQDKVIDRIDNCPENAEVTLTDFRAYQTVVLDPEGDAQIDPNWVVLNQGMEIVQTMNSDPGLAVGYTAFSGVDFEGTFHVNTVTDDDYAGFIFGYQDSSSFYVVMWKQTEQTYWQATPFRAVAEPGIQLKAVKSKSGPGEHLRNSLWHTGDTNDQVRLLWKDPRNVGWKDKVSYRWYLQHRPQVGYIRARFYEGTELVADSGVTIDTTMRGGRLGVFCFSQENIIWSNLKYRCNDTIPEDFQFSTQHGVNDSL

Target ORF information:

RefSeq Version	XM_020457463.1
Organism	Oncorhynchus kisutch(coho salmon)
Definition	Oncorhynchus kisutch thrombospondin-4-B-like (LOC109868058), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_020457463.1

ORF Insert Sequence:

ATGGGGCTGT GGACGATGCT GATCGTGGCT TCGCAGCTGA TCTTAAACCT GTGCTCAGAC 
GGAGCCCAGG GATCAGTCTA TGATCTCCTA AACTCCCCGG ACTGTCTGCC AGACCTGCTG 
CAGGGCGGCC TGGTAGAGCA GGGGGTGAAT GAGGCGTTCA TCCTCACCAC CTTTAAACTG 
CAGCCCAAGA CTGGCACCAC CGTGTTTGGT CTGTACAACC CCCGGGACAA CAGCAAGTAC 
TTTGAGTTCA CCGTGCTGGG GAAACTCAAC AGAGCTGTGC TGCGTTACCT GAGGAGTGAT 
CGGAGGATGA GCTCTGTGAC CTTCAACAAC ATCCAACTGG CGGACGGTCA GCGCCACCGC 
CTCCTCTTCC ACCTGAAGGG GATGCAGCAG GGCCCAGGGG CGGTGGAGCT GCACCTGGAC 
TGCAGACTGG TGGAGACGGT CAGGGATCTC CCCTCCGTGT TTCAGGGCCT GCCTGCAGGC 
TACGGCGTGG TGGAGCTGAA GAGCATGCAG GGCAAAGCAG AGGAGGAGCT GGAGGAATTG 
AAACTGGTGG TGGGAGACAC GTTCGAGAAC GTAGCCTCTC TACAAAACTG CCACCAACAA 
GGAGACTCGG TGCAGACTCT GGGGGTCAAC ACAAAGCAAC TCTCCAATCA AATGCTTGAG 
CTCACCCAGG TGATAAATGA GCTGAAAGAT GTCCTTATTC AGCAGGTCAA AGAGACATCT 
TTTCTCAGAA ACACCATCTC TGAGTGCCAG GCTTGTGGAC TGGGTGGAAG CGAGGTCATT 
GAGCCAAAGT GTGCCCCTGG TGTTTGTTTC CGTGACGACA TGTGTATTGA GACGGAGGAG 
GGTGTTGAGT GTGGACCCTG TCCTGATGGA TACACAGGGG ATGGCTTCAG CTGTGATGAT 
GTGGATGAGT GTCAGTTTAA CCCTTGCTTC GCCGGGGTAA AGTGTGTGAA CACAGCTCCA 
GGGTTTCGCT GTGACGCATG TCCTCTGGGC TACACTGGTC TGGCTGTGGA GGGGGTGGGA 
GTGGTCTACG CACAGACAAA CAAACAGGTC TGTGACGACA TTGATGAGTG CAAAGGACCC 
AACAACGGAG GGTGCACTGC TGACTCTCTC TGTCACAACT CTGCGGGTTC ATACGTCTGT 
GGGGGCTGTA AAACAGGCTA CATAGGGGGC CAGGTGAAGG GCTGTAAGCC AGAGAGGAGC 
TGTGGTAACA GTCGGACCAA CCCCTGTGAT GTCAACGCCC AGTGTATCCA AGAAAGAGAC 
GGCTCCATCA CCTGTCAGTG TGGGATTGGC TGGGCTGGTA ATGGCTACCT ATGTGGGAAG 
GACACAGACA TTGATGGATA CCCTGATGAG AAACTCAAGT GCAAGGATAT GGTCTGTAAA 
AAGGATAACT GCATGCGTGT TCCCAACTCT GGCCAAGAAG ATGCTGACGA GGACGGACAA 
GGGGACGCTT GTGACGATGA CGCTGATGGA GACGGTATTC TGAATGAACA GGATAACTGC 
TGGCTGAAGC CTAACGTGGA CCAGAGGAAT AGTGACAAGG ACAACCACGG TGATGCCTGT 
GACAACTGTC GTACGGTGGA GAACCCCGAT CAGAGGGACA CTGACGGTGA CAGGAAAGGA 
GACGCCTGCG ATGATGACAT GGATGGAGAC GGCTTGAAGA ACTTCCTGGA TAACTGCCAG 
ATGGTGGTGA ACCGGGACCA GTTGGACCGG GATGGAGACG GAGTGGGAGA CGCCTGTGAC 
AGCTGCCCCG ATATTCCTAA TCCTAACCAG TCCGATATCG ATGATGACCT GGTTGGGGAT 
TCTTGTGACA CAAACCAAGA CAGTGATGGT GACGGTCACC AGGACACCAA GGACAACTGT 
CCCAACGTGA TCAACAGCTC CCAGCTGGAC ACAGACAAAG ACGGCCTGGG GGATGAGTGT 
GACGATGACG ATGACAACGA CAGCATCCCT GACTTCCTGC CACCAGGACC CGACAACTGT 
AGACTGGTGC CCAACCCCGA CCAGCTGGAT GAGAACAATG ACGGTGTTGG GGATGTGTGT 
GAGTCAGACT TTGACCAGGA CAAGGTGATT GACAGAATAG ACAACTGTCC AGAGAACGCT 
GAGGTCACTC TGACAGACTT CAGAGCCTAT CAGACGGTGG TGCTGGACCC CGAGGGTGAC 
GCCCAGATTG ACCCCAACTG GGTTGTCCTC AATCAGGGAA TGGAGATTGT GCAGACCATG 
AACAGTGACC CTGGACTGGC TGTGGGTTAC ACTGCGTTCA GTGGGGTGGA CTTTGAGGGG 
ACGTTCCATG TAAACACAGT TACGGACGAT GACTACGCAG GCTTCATCTT TGGCTACCAG 
GACTCCTCCT CCTTCTACGT GGTCATGTGG AAACAGACTG AGCAGACCTA CTGGCAGGCC 
ACACCCTTCA GAGCAGTGGC CGAGCCTGGC ATCCAGCTCA AGGCTGTGAA GTCTAAGTCG 
GGTCCAGGTG AGCACCTGAG GAACTCCCTT TGGCACACAG GTGACACCAA CGACCAGGTG 
CGTCTGTTGT GGAAGGACCC CAGGAACGTG GGCTGGAAGG ACAAGGTTTC CTACCGCTGG 
TACCTGCAGC ACCGGCCCCA GGTTGGATAC ATCAGGGCCC GTTTCTATGA AGGTACAGAA 
CTGGTGGCTG ACTCTGGTGT GACCATTGAC ACCACCATGA GAGGAGGGCG ACTGGGCGTT 
TTCTGTTTCT CTCAGGAAAA CATAATCTGG TCCAACCTAA AGTATCGCTG CAATGACACC 
ATCCCTGAGG ACTTCCAGTT CAGCACGCAG CACGGCGTCA ACGACAGTCT CTAA