gad2 cDNA ORF clone, Nothobranchius furzeri(turquoise killifish)

Gene
Clones
gRNAs

Summary

Gene Symbol	gad2
Entrez Gene ID	107383043
Full Name	glutamate decarboxylase 2
Gene Type	protein-coding
Organism	Nothobranchius furzeri(turquoise killifish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_015955457.1	XP_015810943.1	glutamate decarboxylase 2

Pathways from BioSystems
Gene Ontology

The following gad2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the gad2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ONo13571	XM_015955457.1 Latest version!	Nothobranchius furzeri glutamate decarboxylase 2 (gad2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

gad2 ( XM_015955457.1 ) cDNA ORF clone, Nothobranchius furzeri(turquoise killifish) -> XP_015810943.1 Nothobranchius furzeri glutamate decarboxylase 2 (gad2), mRNA.

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CloneID	ONo13571
Clone ID Related Accession (Same CDS sequence)	XM_015955457.1
Accession Version	XM_015955457.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1755bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-03-17
Organism	Nothobranchius furzeri(turquoise killifish)
Product	glutamate decarboxylase 2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015443551.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Nothobranchius furzeri Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTTCAC ACGGGTTCTG GTCTCTCGGC GGGGATAACG CGGGGACCAA CACCGGCAGT 
CAGAGCCCCA GCACGCCCAG GGCTTGGTGC CAAGCGGCGG CCCAGAAATT ATCCGGAGGA 
ATTGGATCTA AATTATGTGC GCTGCTGAAC GTCGGGGAGG TGGAAAAAAC CGCCGACCCG 
GCCGCCAAAC AGCCGCCGCA GACTGCTGCA GGAAGCTGCG GCTGCAACAA ACCTTGCACC 
TGCACCAAAC CCGACGTGGT CTTCTCAGAC CTCTATTCAA CAGACCTGTT ACCTGCCTTG 
GACGGTGACA CCAAAACGAT GACCTTCCTG CAGGAGGTGG TGGACCTTCT TCTCGCGTAC 
ATAGTTGAAT CTTTCGACCG GGAAACGAAA GTCATTGATT TTCACTATCC AAACGAGCTG 
CTGCAGCTGA ACAACTGGGA GCTGCAGGAC GAGCCCGCCA CCCTGGATGA CATCTTGATC 
AGCTGCCGCG CCACTCTCAA ATACGCCATC AAAACTGCCC ACCCCAGGTA CTTCAATCAG 
CTCTCCACAG GATTAGACAT GGTTGGACTG GCAGCTGATT GGCTGACATC CACTGCCAAC 
ACCAACATGT TCACTTATGA GGTGGCTCCT GTTTTCGTGC TGCTGGAATA CGTCACCCTG 
AAAAAGATGA GGGAGATCAT TGGCTGGCCT GACGGCCGAG GAGACGGCAT CTTTTCCCCT 
GGTGGCGCTA TCTCCAACAT GTATGCCATG CTCTTAGCCC GCTTCAAGAT GTTCCCTGAA 
GTGAAGGAGA AGGGGATGTC ATCCGTTCCT CGACTGGCGG CCTTCACGTC CGAACACAGC 
CATTTCTCCA TCAAGAAAGG GGCAGCTGCT CTGGGCATCG GGACTGAGAG TGTGATCTGT 
ATCAAGGCAG ATGAATGTGG TAAACTGATC CCTGCTGACC TTGAGAGGAG AATACTGGAG 
GCGAAACAGA AGGGCTTTGT TCCTTTCTTT GTGAGCGCGA CAGCTGGAAC AACAGTGTAC 
GGAGCCTTTG ACCCTCTCAT CGCCATCTCC GACATCTGCA GGAAGTACAA CGTCTGGATG 
CACGTGGATG GAGCGTGGGG AGGAAGTCTG CTCATGTCTG GGAGACACCG GTGGAAGCTA 
GATGGAGTCG AAAGAGCCAA CTCTGTCACA TGGAACCCCC ACAAAATGAT GTCTGTTCCC 
CTGCAGTGTT CTGCCCTGCT GGTCCGAGAG GAGGGTTTGA TGCAGAACTG CAACCAGATG 
CACGCCTGCT ACCTGTTCCA GCAGGACAAA CACTATGACC TGTCCTACGA CACGGGAGAC 
AAAGCGTTGC AGTGTGGACG CCATGTGGAC ATCTTTAAGC TGTGGCTCAT GTGGAGAGCA 
AAGGGCACCA TAGGCTTCGA GGCTCAAATT GACAAGTGTC TGGAGCTGTC AGAGTACCTT 
TACAACAAGA TCAAAGACCG AGAAGGATAC CAGATGGTCT TTGATGGGAA ACCTCAGCAC 
ACCAACGTCT GTTTCTGGTA CCTCCCCCCT GGGATTCGCT ACATGGAGGA CAAAGAGGAG 
CAAAAGAAAC GTTTGCACAA GGTGGCTCCA GTGATAAAAG CTAGGATGAT GGAGTATGGA 
ACGACCATGG TGAGCTACCA GCCTCAAGGA GACAAGGTCA ACTTCTTCCG CATGGTGATC 
TCAAACCCCG CTGCTACCTT TGAGGACATC GACTTCCTTA TCGAGGAAAT TGAGCGACTC 
GGCCACGATC TATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015810943.1
CDS	673..2427
Translation	MASHGFWSLGGDNAGTNTGSQSPSTPRAWCQAAAQKLSGGIGSKLCALLNVGEVEKTADPAAKQPPQTAAGSCGCNKPCTCTKPDVVFSDLYSTDLLPALDGDTKTMTFLQEVVDLLLAYIVESFDRETKVIDFHYPNELLQLNNWELQDEPATLDDILISCRATLKYAIKTAHPRYFNQLSTGLDMVGLAADWLTSTANTNMFTYEVAPVFVLLEYVTLKKMREIIGWPDGRGDGIFSPGGAISNMYAMLLARFKMFPEVKEKGMSSVPRLAAFTSEHSHFSIKKGAAALGIGTESVICIKADECGKLIPADLERRILEAKQKGFVPFFVSATAGTTVYGAFDPLIAISDICRKYNVWMHVDGAWGGSLLMSGRHRWKLDGVERANSVTWNPHKMMSVPLQCSALLVREEGLMQNCNQMHACYLFQQDKHYDLSYDTGDKALQCGRHVDIFKLWLMWRAKGTIGFEAQIDKCLELSEYLYNKIKDREGYQMVFDGKPQHTNVCFWYLPPGIRYMEDKEEQKKRLHKVAPVIKARMMEYGTTMVSYQPQGDKVNFFRMVISNPAATFEDIDFLIEEIERLGHDL

Target ORF information:

RefSeq Version	XM_015955457.1
Organism	Nothobranchius furzeri(turquoise killifish)
Definition	Nothobranchius furzeri glutamate decarboxylase 2 (gad2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015955457.1

ORF Insert Sequence:

ATGGCTTCAC ACGGGTTCTG GTCTCTCGGC GGGGATAACG CGGGGACCAA CACCGGCAGT 
CAGAGCCCCA GCACGCCCAG GGCTTGGTGC CAAGCGGCGG CCCAGAAATT ATCCGGAGGA 
ATTGGATCTA AATTATGTGC GCTGCTGAAC GTCGGGGAGG TGGAAAAAAC CGCCGACCCG 
GCCGCCAAAC AGCCGCCGCA GACTGCTGCA GGAAGCTGCG GCTGCAACAA ACCTTGCACC 
TGCACCAAAC CCGACGTGGT CTTCTCAGAC CTCTATTCAA CAGACCTGTT ACCTGCCTTG 
GACGGTGACA CCAAAACGAT GACCTTCCTG CAGGAGGTGG TGGACCTTCT TCTCGCGTAC 
ATAGTTGAAT CTTTCGACCG GGAAACGAAA GTCATTGATT TTCACTATCC AAACGAGCTG 
CTGCAGCTGA ACAACTGGGA GCTGCAGGAC GAGCCCGCCA CCCTGGATGA CATCTTGATC 
AGCTGCCGCG CCACTCTCAA ATACGCCATC AAAACTGCCC ACCCCAGGTA CTTCAATCAG 
CTCTCCACAG GATTAGACAT GGTTGGACTG GCAGCTGATT GGCTGACATC CACTGCCAAC 
ACCAACATGT TCACTTATGA GGTGGCTCCT GTTTTCGTGC TGCTGGAATA CGTCACCCTG 
AAAAAGATGA GGGAGATCAT TGGCTGGCCT GACGGCCGAG GAGACGGCAT CTTTTCCCCT 
GGTGGCGCTA TCTCCAACAT GTATGCCATG CTCTTAGCCC GCTTCAAGAT GTTCCCTGAA 
GTGAAGGAGA AGGGGATGTC ATCCGTTCCT CGACTGGCGG CCTTCACGTC CGAACACAGC 
CATTTCTCCA TCAAGAAAGG GGCAGCTGCT CTGGGCATCG GGACTGAGAG TGTGATCTGT 
ATCAAGGCAG ATGAATGTGG TAAACTGATC CCTGCTGACC TTGAGAGGAG AATACTGGAG 
GCGAAACAGA AGGGCTTTGT TCCTTTCTTT GTGAGCGCGA CAGCTGGAAC AACAGTGTAC 
GGAGCCTTTG ACCCTCTCAT CGCCATCTCC GACATCTGCA GGAAGTACAA CGTCTGGATG 
CACGTGGATG GAGCGTGGGG AGGAAGTCTG CTCATGTCTG GGAGACACCG GTGGAAGCTA 
GATGGAGTCG AAAGAGCCAA CTCTGTCACA TGGAACCCCC ACAAAATGAT GTCTGTTCCC 
CTGCAGTGTT CTGCCCTGCT GGTCCGAGAG GAGGGTTTGA TGCAGAACTG CAACCAGATG 
CACGCCTGCT ACCTGTTCCA GCAGGACAAA CACTATGACC TGTCCTACGA CACGGGAGAC 
AAAGCGTTGC AGTGTGGACG CCATGTGGAC ATCTTTAAGC TGTGGCTCAT GTGGAGAGCA 
AAGGGCACCA TAGGCTTCGA GGCTCAAATT GACAAGTGTC TGGAGCTGTC AGAGTACCTT 
TACAACAAGA TCAAAGACCG AGAAGGATAC CAGATGGTCT TTGATGGGAA ACCTCAGCAC 
ACCAACGTCT GTTTCTGGTA CCTCCCCCCT GGGATTCGCT ACATGGAGGA CAAAGAGGAG 
CAAAAGAAAC GTTTGCACAA GGTGGCTCCA GTGATAAAAG CTAGGATGAT GGAGTATGGA 
ACGACCATGG TGAGCTACCA GCCTCAAGGA GACAAGGTCA ACTTCTTCCG CATGGTGATC 
TCAAACCCCG CTGCTACCTT TGAGGACATC GACTTCCTTA TCGAGGAAAT TGAGCGACTC 
GGCCACGATC TATAA