PIK3CA cDNA ORF clone, Nestor notabilis(Kea)

Gene
Clones
gRNAs

Summary

Gene Symbol	PIK3CA
Entrez Gene ID	104408510
Full Name	phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit alpha
Gene Type	protein-coding
Organism	Nestor notabilis(Kea)

mRNA and Protein(s)

mRNA	Protein	Name
XM_010018147.1	XP_010016449.1	LOW QUALITY PROTEIN: phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit alpha isoform

Pathways from BioSystems
Gene Ontology

The following PIK3CA gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PIK3CA cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ONe110671	XM_010018147.1 Latest version!	Nestor notabilis phosphatidylinositol-4,5-bisphosphate 3-kinase, catalytic subunit alpha (PIK3CA), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PIK3CA ( XM_010018147.1 ) cDNA ORF clone, Nestor notabilis(Kea) -> XP_010016449.1 Nestor notabilis phosphatidylinositol-4,5-bisphosphate 3-kinase, catalytic subunit alpha (PIK3CA), mRNA.

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CloneID	ONe110671
Clone ID Related Accession (Same CDS sequence)	XM_010018147.1
Accession Version	XM_010018147.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3225bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-10-30
Organism	Nestor notabilis(Kea)
Product	LOW QUALITY PROTEIN: phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit alpha isoform
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_009912191.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Nestor notabilis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## frameshifts :: corrected 1 indel ##RefSeq-Attributes-END##

ATGCCTCCAC GACCATCATC TGGTGAACTA TGGGGCATCC ACTTGATGCC ACCGAGGATC 
CTTGTGGAGT GTCTTCTCCC AAATGGAATG ATAGTGACTC TAGAATGCCT CCGTGAGGCC 
ACCTTACTAA CTATTAAACA TGAACTCTTT AAGGAAGCAA GAAAATATCC TCTCTATCAA 
CTCCTTCAAG ATGAATCTTC TTATATTTTT GTAAGTGTTA CACAAGAAGC AGAAAGAGAA 
GAATTTTTTG ATGAAACACG GAGACTTTGT GACCTGCGAC TGTTTCAGCC TTTTCTAAAA 
GTTATTGAAC CAGTAGGTAA CAGAGAAGAA AAGATTCTCA ATAGAGAAAT AGGTTTTGCT 
ATTGGAATGC CTGTCTGTGA ATTTGACATG GTTAAGGATC CAGAAGTACA AGACTTCAGA 
AGAAATATTC TTAATGTTTG TAAAGAAGCA GTGGATCTTC GAGATGCCAA TGCACCACAT 
AGTAGAGCAT TATATGTCTG TCCTCCAAAT GTAGAGTCTT CACCTGAACT ACCCAAACAC 
ATATACAATA AACTAGACAA AGGGCAAATT ATAGTTGTGA TATGGGTAAT AGTCTCACCA 
AACAATGATA AGCAGAAATA CACCTTAAAA ATCAATCATG ACTGTGTGCC TGAACAAGTT 
ATTGCTGAAG CAATTAGGAA GAAAACACGA AGTATGTTGC TGTCATCAGA ACAACTGAAA 
CTTTGCGTCT TGGAGTACCA AGGCAAATAC ATTCTAAAAG TGTGTGGCTG TGATGAATAC 
TTGCTAGAAA AATATCCACT GAGCCAGTAT AAGTATATAA GAAGCTGTAT AATGCTGGGT 
CGCATGCCCA ATCTGATGCT GATGGCTAAG GAAAGCCTAT ATACCCAGCT GCCACTGGAC 
ACCTTTACAA TGCCATCTTA TTCCAGGCGT ATCTCTACAG CTACACCCTA CATGAATGGA 
GAAGCTACAG CCAAATCCCT CTGGACTATA AACAGTGCTC TCAGAATAAG AATCCTCTGT 
GCAACCTATG TAAATGTGAA CATTAGAGAC ATAGACAAGA TCTATGTAAG AACAGGTATC 
TACCATGGAG GGGAACCTTT GTGTGACAAT GTGAATACTC AAAGGGTACC TTGTTCTAAT 
CCCAGGTGGA ACGAATGGCT ATTGTATGAC ATGTACATTC TTGATCTTCC ACGTGCTGCT 
CGCCTGTGCC TTTCTATCTG TTCTGTTAAA GGCCGTAAGG GTGCAAAAGA GGAGCACTGT 
CCACTGGCTT GGGGAAATAT AAACATGTTT GATTACACAG ACACTCTTGT ATCTGGGAAA 
ATGGCTTTGA ATCTTTGGGC AGTACCTCAT GGCCTGGAGG ATTTGTTAAA TCCTATTGGT 
GTTACTGGAT CAAATCCAAA TAAGGAAACT CCATGTCTAG AGCTGGAATT TGATTGGTTT 
AGCAATCCTG TGAAGTTTCC AGATATGACA GTGATTGAAG AGCATGCCAA TTGGACTATA 
TCACGCGAAC TGGGGTTTAA CTACAGCTAT GCGGGGCTGA GTAACAGAAT AGCTAGAGAT 
AACGAATTAA GAGAAAGTGA CAAGGAGCAA CTGCGAGCTA TATGTACACG AGATCCTTTG 
TCTGAAATCA CTGAGCAAGA GAAGGACTTC CTCTGGAGCC ACAGACACTA TTGCGTGAAT 
ACTCCAGAAA TTCTGCCCAA ATTACTTCTG TCTGTGAAAT GGAATTCTAG AGATGAAGTA 
GCCCAGATGT ACTGTTTGGT AAAAGATTGG CCTCCAATCA AGCCAGAGCA GGCAATGGAG 
CTTTTGGACT GTAATTATCC AGATCCAATG GTGCGAGCTT TTGCAGTTCG GTGTCTAGAG 
AAGTACTTGA CAGATGACAA ACTGTCTCAG TACTTAATCC AGCTGGTACA GGTTCTGAAA 
TATGAACAGT ACTTGGATAA TCAGCTTGTA AGATTTTTAC TCAAGAAGGC ACTGACCAAT 
CAAAGGATAG GACACTTCTT CTTTTGGCAT TTAAAGTCTG AAATGCACAA TAAAACTGTA 
AGTCAGAGGT TTGGTTTACT TCTGGAGTCC TATTGTCGAG CATGTGGAAT GTACCTAAAA 
CATCTGAGCA GGCAGGTGGA AGCTATGGAG AAGTTGATTA ACCTCACTGA TATTCTCAAG 
CAGGAAAAAA AAGATGAGAC ACAGAAGGTA CAGATGAAGT TTCTTGTTGA ACAAATGAGG 
CGGCCAGATT TTATGGATGC CTTACAAGGT TTTATCTCTC CTCTTAATCC TGCGCATCAA 
CTGGGAAATC TTCGGCTTGA GGAGTGCAGG ATAATGTCAT CTGCAAAAAG GCCACTGTGG 
TTGAACTGGG AAAACCCGGA TATTATGTCT GAATTGTTAT TTCAGAACAA TGAGATAATC 
TTTAAAAATG GAGACGACTT GCGTCAGGAT ATGCTGACAC TTCAGATAAT AAGAATTATG 
GAAAACATCT GGCAAAATCA GGGTCTGGAT CTTCGGATGT TGCCTTATGG TTGTTTGTCT 
ATTGGTGACT GTGTGGGACT CATCGAGGTA GTCAGGAGCT CTCATACCAT CATGCAGATT 
CAATGTAAAG GAGGCTTAAA GGGAGCATTG CAGTTCAACA GCCATACATT ACATCAGTGG 
CTCAAGGACA AGAACAAAGG AGAAATGTAT GATGCAGCTA TTGACTTGTT TACACGTTCT 
TGTGCTGGCT ACTGTGTTGC TACCTTTATA CTGGGCATTG GTGATCGCCA CAATAGTAAC 
ATCATGGTAA AAGATGATGG ACAACTGTTT CACATTGACT TTGGGCACTT CCTGGACCAC 
AAGAAGAAAA AATTTGGTTA TAAAAGGGAG CGTGTGCCAT TTGTCTTAAC ACAGGACTTC 
TTAATAGTGA TTAGTAAAGG AGCCCAAGAA TGTACTAAAA CAAGGGAGTT TGAAAGGTTC 
CAGGAGATGT GTTATAAGGC TTATCTAGCA ATTCGGCAGC ATGCCAATCT CTTCATAAAT 
CTTTTCTCCA TGATGNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNATGCC AGAACTGCAG 
TCCTTTGACG ATATTGCATA CATTCGGAAG ACCCTTGCAT TGGACAAAAC TGAGCAGGAA 
GCTCTGGAGT ACTTCATGAA GCAAATGAAT GATGCTCACC ATGGTGGCTG GACAACAAAA 
ATGGACTGGA TCTTTCACAC AATAAAACAA CATGCTTTGA ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010016449.1
CDS	1..3225
Translation	MPPRPSSGELWGIHLMPPRILVECLLPNGMIVTLECLREATLLTIKHELFKEARKYPLYQLLQDESSYIFVSVTQEAEREEFFDETRRLCDLRLFQPFLKVIEPVGNREEKILNREIGFAIGMPVCEFDMVKDPEVQDFRRNILNVCKEAVDLRDANAPHSRALYVCPPNVESSPELPKHIYNKLDKGQIIVVIWVIVSPNNDKQKYTLKINHDCVPEQVIAEAIRKKTRSMLLSSEQLKLCVLEYQGKYILKVCGCDEYLLEKYPLSQYKYIRSCIMLGRMPNLMLMAKESLYTQLPLDTFTMPSYSRRISTATPYMNGEATAKSLWTINSALRIRILCATYVNVNIRDIDKIYVRTGIYHGGEPLCDNVNTQRVPCSNPRWNEWLLYDMYILDLPRAARLCLSICSVKGRKGAKEEHCPLAWGNINMFDYTDTLVSGKMALNLWAVPHGLEDLLNPIGVTGSNPNKETPCLELEFDWFSNPVKFPDMTVIEEHANWTISRELGFNYSYAGLSNRIARDNELRESDKEQLRAICTRDPLSEITEQEKDFLWSHRHYCVNTPEILPKLLLSVKWNSRDEVAQMYCLVKDWPPIKPEQAMELLDCNYPDPMVRAFAVRCLEKYLTDDKLSQYLIQLVQVLKYEQYLDNQLVRFLLKKALTNQRIGHFFFWHLKSEMHNKTVSQRFGLLLESYCRACGMYLKHLSRQVEAMEKLINLTDILKQEKKDETQKVQMKFLVEQMRRPDFMDALQGFISPLNPAHQLGNLRLEECRIMSSAKRPLWLNWENPDIMSELLFQNNEIIFKNGDDLRQDMLTLQIIRIMENIWQNQGLDLRMLPYGCLSIGDCVGLIEVVRSSHTIMQIQCKGGLKGALQFNSHTLHQWLKDKNKGEMYDAAIDLFTRSCAGYCVATFILGIGDRHNSNIMVKDDGQLFHIDFGHFLDHKKKKFGYKRERVPFVLTQDFLIVISKGAQECTKTREFERFQEMCYKAYLAIRQHANLFINLFSMMnullnullnullnullnullnullnullnullnullnullMPELQSFDDIAYIRKTLALDKTEQEALEYFMKQMNDAHHGGWTTKMDWIFHTIKQHALN

Target ORF information:

RefSeq Version	XM_010018147.1
Organism	Nestor notabilis(Kea)
Definition	Nestor notabilis phosphatidylinositol-4,5-bisphosphate 3-kinase, catalytic subunit alpha (PIK3CA), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010018147.1

ORF Insert Sequence:

ATGCCTCCAC GACCATCATC TGGTGAACTA TGGGGCATCC ACTTGATGCC ACCGAGGATC 
CTTGTGGAGT GTCTTCTCCC AAATGGAATG ATAGTGACTC TAGAATGCCT CCGTGAGGCC 
ACCTTACTAA CTATTAAACA TGAACTCTTT AAGGAAGCAA GAAAATATCC TCTCTATCAA 
CTCCTTCAAG ATGAATCTTC TTATATTTTT GTAAGTGTTA CACAAGAAGC AGAAAGAGAA 
GAATTTTTTG ATGAAACACG GAGACTTTGT GACCTGCGAC TGTTTCAGCC TTTTCTAAAA 
GTTATTGAAC CAGTAGGTAA CAGAGAAGAA AAGATTCTCA ATAGAGAAAT AGGTTTTGCT 
ATTGGAATGC CTGTCTGTGA ATTTGACATG GTTAAGGATC CAGAAGTACA AGACTTCAGA 
AGAAATATTC TTAATGTTTG TAAAGAAGCA GTGGATCTTC GAGATGCCAA TGCACCACAT 
AGTAGAGCAT TATATGTCTG TCCTCCAAAT GTAGAGTCTT CACCTGAACT ACCCAAACAC 
ATATACAATA AACTAGACAA AGGGCAAATT ATAGTTGTGA TATGGGTAAT AGTCTCACCA 
AACAATGATA AGCAGAAATA CACCTTAAAA ATCAATCATG ACTGTGTGCC TGAACAAGTT 
ATTGCTGAAG CAATTAGGAA GAAAACACGA AGTATGTTGC TGTCATCAGA ACAACTGAAA 
CTTTGCGTCT TGGAGTACCA AGGCAAATAC ATTCTAAAAG TGTGTGGCTG TGATGAATAC 
TTGCTAGAAA AATATCCACT GAGCCAGTAT AAGTATATAA GAAGCTGTAT AATGCTGGGT 
CGCATGCCCA ATCTGATGCT GATGGCTAAG GAAAGCCTAT ATACCCAGCT GCCACTGGAC 
ACCTTTACAA TGCCATCTTA TTCCAGGCGT ATCTCTACAG CTACACCCTA CATGAATGGA 
GAAGCTACAG CCAAATCCCT CTGGACTATA AACAGTGCTC TCAGAATAAG AATCCTCTGT 
GCAACCTATG TAAATGTGAA CATTAGAGAC ATAGACAAGA TCTATGTAAG AACAGGTATC 
TACCATGGAG GGGAACCTTT GTGTGACAAT GTGAATACTC AAAGGGTACC TTGTTCTAAT 
CCCAGGTGGA ACGAATGGCT ATTGTATGAC ATGTACATTC TTGATCTTCC ACGTGCTGCT 
CGCCTGTGCC TTTCTATCTG TTCTGTTAAA GGCCGTAAGG GTGCAAAAGA GGAGCACTGT 
CCACTGGCTT GGGGAAATAT AAACATGTTT GATTACACAG ACACTCTTGT ATCTGGGAAA 
ATGGCTTTGA ATCTTTGGGC AGTACCTCAT GGCCTGGAGG ATTTGTTAAA TCCTATTGGT 
GTTACTGGAT CAAATCCAAA TAAGGAAACT CCATGTCTAG AGCTGGAATT TGATTGGTTT 
AGCAATCCTG TGAAGTTTCC AGATATGACA GTGATTGAAG AGCATGCCAA TTGGACTATA 
TCACGCGAAC TGGGGTTTAA CTACAGCTAT GCGGGGCTGA GTAACAGAAT AGCTAGAGAT 
AACGAATTAA GAGAAAGTGA CAAGGAGCAA CTGCGAGCTA TATGTACACG AGATCCTTTG 
TCTGAAATCA CTGAGCAAGA GAAGGACTTC CTCTGGAGCC ACAGACACTA TTGCGTGAAT 
ACTCCAGAAA TTCTGCCCAA ATTACTTCTG TCTGTGAAAT GGAATTCTAG AGATGAAGTA 
GCCCAGATGT ACTGTTTGGT AAAAGATTGG CCTCCAATCA AGCCAGAGCA GGCAATGGAG 
CTTTTGGACT GTAATTATCC AGATCCAATG GTGCGAGCTT TTGCAGTTCG GTGTCTAGAG 
AAGTACTTGA CAGATGACAA ACTGTCTCAG TACTTAATCC AGCTGGTACA GGTTCTGAAA 
TATGAACAGT ACTTGGATAA TCAGCTTGTA AGATTTTTAC TCAAGAAGGC ACTGACCAAT 
CAAAGGATAG GACACTTCTT CTTTTGGCAT TTAAAGTCTG AAATGCACAA TAAAACTGTA 
AGTCAGAGGT TTGGTTTACT TCTGGAGTCC TATTGTCGAG CATGTGGAAT GTACCTAAAA 
CATCTGAGCA GGCAGGTGGA AGCTATGGAG AAGTTGATTA ACCTCACTGA TATTCTCAAG 
CAGGAAAAAA AAGATGAGAC ACAGAAGGTA CAGATGAAGT TTCTTGTTGA ACAAATGAGG 
CGGCCAGATT TTATGGATGC CTTACAAGGT TTTATCTCTC CTCTTAATCC TGCGCATCAA 
CTGGGAAATC TTCGGCTTGA GGAGTGCAGG ATAATGTCAT CTGCAAAAAG GCCACTGTGG 
TTGAACTGGG AAAACCCGGA TATTATGTCT GAATTGTTAT TTCAGAACAA TGAGATAATC 
TTTAAAAATG GAGACGACTT GCGTCAGGAT ATGCTGACAC TTCAGATAAT AAGAATTATG 
GAAAACATCT GGCAAAATCA GGGTCTGGAT CTTCGGATGT TGCCTTATGG TTGTTTGTCT 
ATTGGTGACT GTGTGGGACT CATCGAGGTA GTCAGGAGCT CTCATACCAT CATGCAGATT 
CAATGTAAAG GAGGCTTAAA GGGAGCATTG CAGTTCAACA GCCATACATT ACATCAGTGG 
CTCAAGGACA AGAACAAAGG AGAAATGTAT GATGCAGCTA TTGACTTGTT TACACGTTCT 
TGTGCTGGCT ACTGTGTTGC TACCTTTATA CTGGGCATTG GTGATCGCCA CAATAGTAAC 
ATCATGGTAA AAGATGATGG ACAACTGTTT CACATTGACT TTGGGCACTT CCTGGACCAC 
AAGAAGAAAA AATTTGGTTA TAAAAGGGAG CGTGTGCCAT TTGTCTTAAC ACAGGACTTC 
TTAATAGTGA TTAGTAAAGG AGCCCAAGAA TGTACTAAAA CAAGGGAGTT TGAAAGGTTC 
CAGGAGATGT GTTATAAGGC TTATCTAGCA ATTCGGCAGC ATGCCAATCT CTTCATAAAT 
CTTTTCTCCA TGATGNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNATGCC AGAACTGCAG 
TCCTTTGACG ATATTGCATA CATTCGGAAG ACCCTTGCAT TGGACAAAAC TGAGCAGGAA 
GCTCTGGAGT ACTTCATGAA GCAAATGAAT GATGCTCACC ATGGTGGCTG GACAACAAAA 
ATGGACTGGA TCTTTCACAC AATAAAACAA CATGCTTTGA ACTGA