ETFDH cDNA ORF clone, Nestor notabilis(Kea)

Gene
Clones
gRNAs

Summary

Gene Symbol	ETFDH
Entrez Gene ID	104402854
Full Name	electron transfer flavoprotein dehydrogenase
Gene Type	protein-coding
Organism	Nestor notabilis(Kea)

mRNA and Protein(s)

mRNA	Protein	Name
XM_010010839.1	XP_010009141.1	electron transfer flavoprotein-ubiquinone oxidoreductase, mitochondrial

Pathways from BioSystems
Gene Ontology

The following ETFDH gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ETFDH cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ONe112604	XM_010010839.1 Latest version!	Nestor notabilis electron-transferring-flavoprotein dehydrogenase (ETFDH), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ETFDH ( XM_010010839.1 ) cDNA ORF clone, Nestor notabilis(Kea) -> XP_010009141.1 Nestor notabilis electron-transferring-flavoprotein dehydrogenase (ETFDH), mRNA.

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CloneID	ONe112604
Clone ID Related Accession (Same CDS sequence)	XM_010010839.1
Accession Version	XM_010010839.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1671bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-10-30
Organism	Nestor notabilis(Kea)
Product	electron transfer flavoprotein-ubiquinone oxidoreductase, mitochondrial
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_009920755.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Nestor notabilis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAAGAT TTGCAGAGGA AGCTGACGTT GTCATTGTTG GAGGAGGCCC TGCGGGTCTC 
TCTGCAGCTA TTCGACTCAA ACAGTTAGCC GCTGAGCATA ACAAAGAAAT ACGTGTTTGC 
CTGGTGGAAA AAGCTTCTCA GATTGGAGCG CATACATTGT CTGGTGCCTG TCTTGAGCCA 
AGGTCCTTAG AAGAACTCTT TCCAGACTGG AAGGAGCGGG GGGCTCCACT TAATACTCCC 
GTAACTGAAG ACAAGTTTGG AATTTTAACA GAGAAATCTA GAATTCCAGT GCCAATTATT 
CCAGGACTTC CAATGGTTAA TCATGGAAAT TACATAGTAC GTCTGGGCCA CTTTGTGAGC 
TGGCTTGGTG AACAAGCAGA AGCATTGGGT GTTGAGATAT ATCCAGGCTA TGCAGCAGCT 
GAGGTTCTTT TTCATGAAGA TGGTAGCGTG AAAGGGATAG CAACTAATGA TGTAGGCATT 
CAGAAGGATG GAGCACCTAA AGCTACCTTT GAAAGAGGCT TGGAACTTCA TGGTAAAGTC 
ACAGTTTTCG CTGAAGGTTG TCATGGACAT CTAGCCAAAC AGATGTACAA AAAGTACAAT 
CTAAGGGAGA ACTGTCAACC CCAGAGCTAT GGCATTGGAC TGAAAGAGTT GTGGACTATT 
GATGAAAAGA AATGGAAACC AGGAAGAGTG GAACATACTG TAGGCTGGCC TTTAGACAGA 
CATACATATG GAGGATCCTT TCTTTATCAC TTAAATGAGA GTGAACCTTT AGTTGCTCTT 
GGATTTGTGG TTGGTTTAGA TTATCAAAAT CCCTATTTGA ATCCATTTAG GGAGTTTCAG 
AGGTGGAAGC ACCATCCTAC TGTACAGCCT ACTTTGGAGG GTGGAACAAG GATTGCCTAT 
GGTGCCAGAG CATTAAATGA AGGTGGTTTA CAGTCAATAC CCAAACTCAC CTTCCCAGGT 
GGAGTATTAA TTGGTTGCAG TCCTGGACTC ATGAATGTTC CTAAGATCAA AGGGACACAT 
ACTGCAATGA AGAGCGGCAT GTTGGCTTCT GAAGCCATCT TTAACCAATT AATCAATGAA 
AATCTCCAGT CAAAGACAAA AGGACTTCAT GTGCAAGAAT ATGAAGAGAA CCTGAAAAAG 
TCCTGGGTCT GGAAGGAACT GTATTCGGTT AGAAACATCC GACCATCCTG CCATAGCATA 
CTTGGTGTGT ATGGAGGAAT GATTTACACC GGTATATTCT ACTGGCTGCT GCGAGGAATG 
GAACCATGGA CATTAAAACA TCCAGGACCA GATTTTGCTC AGCTCAAGCC AGCCAAAGAG 
TGCACGCCTA TTGAATACCC AAAGCCTGAT GGAAAAATCA GTTTTGATCT CCTGTCATCT 
GTGGCTTTAA GTGGAACAAA CCATGAACAT GACCAGCCTG CTCATTTAAC TCTGAAAGAT 
GACAGCATCC CTGTAAGCAG GAATCTGGCT GTATTTGATG GACCGGAACA AAGATTTTGT 
CCAGCAGGAG TTTATGAGTA CATTCCCCTG GAAACAGGAG AAGGATCAAG ACTGCAGATA 
AATGCTCAGA ACTGTGTCCA CTGCAAAACC TGTGATATTA AGGACCCAAG TCAGAACATT 
AACTGGGTAG TGCCTGAAGG TGGAGGAGGA CCAGCTTATA ATGGAATGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010009141.1
CDS	155..1825
Translation	MERFAEEADVVIVGGGPAGLSAAIRLKQLAAEHNKEIRVCLVEKASQIGAHTLSGACLEPRSLEELFPDWKERGAPLNTPVTEDKFGILTEKSRIPVPIIPGLPMVNHGNYIVRLGHFVSWLGEQAEALGVEIYPGYAAAEVLFHEDGSVKGIATNDVGIQKDGAPKATFERGLELHGKVTVFAEGCHGHLAKQMYKKYNLRENCQPQSYGIGLKELWTIDEKKWKPGRVEHTVGWPLDRHTYGGSFLYHLNESEPLVALGFVVGLDYQNPYLNPFREFQRWKHHPTVQPTLEGGTRIAYGARALNEGGLQSIPKLTFPGGVLIGCSPGLMNVPKIKGTHTAMKSGMLASEAIFNQLINENLQSKTKGLHVQEYEENLKKSWVWKELYSVRNIRPSCHSILGVYGGMIYTGIFYWLLRGMEPWTLKHPGPDFAQLKPAKECTPIEYPKPDGKISFDLLSSVALSGTNHEHDQPAHLTLKDDSIPVSRNLAVFDGPEQRFCPAGVYEYIPLETGEGSRLQINAQNCVHCKTCDIKDPSQNINWVVPEGGGGPAYNGM

Target ORF information:

RefSeq Version	XM_010010839.1
Organism	Nestor notabilis(Kea)
Definition	Nestor notabilis electron-transferring-flavoprotein dehydrogenase (ETFDH), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010010839.1

ORF Insert Sequence:

ATGGAAAGAT TTGCAGAGGA AGCTGACGTT GTCATTGTTG GAGGAGGCCC TGCGGGTCTC 
TCTGCAGCTA TTCGACTCAA ACAGTTAGCC GCTGAGCATA ACAAAGAAAT ACGTGTTTGC 
CTGGTGGAAA AAGCTTCTCA GATTGGAGCG CATACATTGT CTGGTGCCTG TCTTGAGCCA 
AGGTCCTTAG AAGAACTCTT TCCAGACTGG AAGGAGCGGG GGGCTCCACT TAATACTCCC 
GTAACTGAAG ACAAGTTTGG AATTTTAACA GAGAAATCTA GAATTCCAGT GCCAATTATT 
CCAGGACTTC CAATGGTTAA TCATGGAAAT TACATAGTAC GTCTGGGCCA CTTTGTGAGC 
TGGCTTGGTG AACAAGCAGA AGCATTGGGT GTTGAGATAT ATCCAGGCTA TGCAGCAGCT 
GAGGTTCTTT TTCATGAAGA TGGTAGCGTG AAAGGGATAG CAACTAATGA TGTAGGCATT 
CAGAAGGATG GAGCACCTAA AGCTACCTTT GAAAGAGGCT TGGAACTTCA TGGTAAAGTC 
ACAGTTTTCG CTGAAGGTTG TCATGGACAT CTAGCCAAAC AGATGTACAA AAAGTACAAT 
CTAAGGGAGA ACTGTCAACC CCAGAGCTAT GGCATTGGAC TGAAAGAGTT GTGGACTATT 
GATGAAAAGA AATGGAAACC AGGAAGAGTG GAACATACTG TAGGCTGGCC TTTAGACAGA 
CATACATATG GAGGATCCTT TCTTTATCAC TTAAATGAGA GTGAACCTTT AGTTGCTCTT 
GGATTTGTGG TTGGTTTAGA TTATCAAAAT CCCTATTTGA ATCCATTTAG GGAGTTTCAG 
AGGTGGAAGC ACCATCCTAC TGTACAGCCT ACTTTGGAGG GTGGAACAAG GATTGCCTAT 
GGTGCCAGAG CATTAAATGA AGGTGGTTTA CAGTCAATAC CCAAACTCAC CTTCCCAGGT 
GGAGTATTAA TTGGTTGCAG TCCTGGACTC ATGAATGTTC CTAAGATCAA AGGGACACAT 
ACTGCAATGA AGAGCGGCAT GTTGGCTTCT GAAGCCATCT TTAACCAATT AATCAATGAA 
AATCTCCAGT CAAAGACAAA AGGACTTCAT GTGCAAGAAT ATGAAGAGAA CCTGAAAAAG 
TCCTGGGTCT GGAAGGAACT GTATTCGGTT AGAAACATCC GACCATCCTG CCATAGCATA 
CTTGGTGTGT ATGGAGGAAT GATTTACACC GGTATATTCT ACTGGCTGCT GCGAGGAATG 
GAACCATGGA CATTAAAACA TCCAGGACCA GATTTTGCTC AGCTCAAGCC AGCCAAAGAG 
TGCACGCCTA TTGAATACCC AAAGCCTGAT GGAAAAATCA GTTTTGATCT CCTGTCATCT 
GTGGCTTTAA GTGGAACAAA CCATGAACAT GACCAGCCTG CTCATTTAAC TCTGAAAGAT 
GACAGCATCC CTGTAAGCAG GAATCTGGCT GTATTTGATG GACCGGAACA AAGATTTTGT 
CCAGCAGGAG TTTATGAGTA CATTCCCCTG GAAACAGGAG AAGGATCAAG ACTGCAGATA 
AATGCTCAGA ACTGTGTCCA CTGCAAAACC TGTGATATTA AGGACCCAAG TCAGAACATT 
AACTGGGTAG TGCCTGAAGG TGGAGGAGGA CCAGCTTATA ATGGAATGTA A