WASHC4 cDNA ORF clone, Neomonachus schauinslandi(Hawaiian monk seal)

Gene
Clones
gRNAs

Summary

Gene Symbol	WASHC4
Entrez Gene ID	110577905
Full Name	WASH complex subunit 4
Gene Type	protein-coding
Organism	Neomonachus schauinslandi(Hawaiian monk seal)

mRNA and Protein(s)

mRNA	Protein	Name
XM_021687370.1	XP_021543045.1	WASH complex subunit 4
XM_021687369.1	XP_021543044.1	WASH complex subunit 4

Pathways from BioSystems
Gene Ontology

The following WASHC4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the WASHC4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ONe140842	XM_021687369.1 Latest version!	Neomonachus schauinslandi WASH complex subunit 4 (WASHC4), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
ONe140842	XM_021687370.1 Latest version!	Neomonachus schauinslandi WASH complex subunit 4 (WASHC4), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

WASHC4 ( XM_021687370.1 ) cDNA ORF clone, Neomonachus schauinslandi(Hawaiian monk seal) -> XP_021543045.1 Neomonachus schauinslandi WASH complex subunit 4 (WASHC4), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ONe140842
Clone ID Related Accession (Same CDS sequence)	XM_021687370.1 , XM_021687369.1
Accession Version	XM_021687370.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3519bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-27
Organism	Neomonachus schauinslandi(Hawaiian monk seal)
Product	WASH complex subunit 4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018734313.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Neomonachus schauinslandi Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGTGG AGACTCTGTC CCCGGACTGG GAGTTCGACC GCGTTGACGA CGGCTCACAG 
AAAATTCATG CTGAAGTCCA ACTTAAGAAT TACGGGAAAT TTCTTGAGGA GTATACATCT 
CAATTGAGAA GAATTGAAGA TGCACTGGAT GATTCAATTG GTGATGTTTG GGATTTCAAT 
CTTGATCCAA TAGCATTAAA GCTTTTGCCT TATGAGCAGT CTTCCCTTTT GGAACTCATA 
AAGACTGAAA ACAAGGTCTT GAACAAAGTC ATCACTGTTT ATGCTGCACT TTGTTGTGAA 
ATCAAGAAAT TAAAGTATGA GGCTGAAACT AAATTTTATA ATGGTCTCTT GTTTTATGGA 
GAAGGAGCTA CAGATTCCAG CATGGTGGAA GGTGATTGCC AAATTCAAAT GGGAAGATTT 
ATTTCATTCT TACAGGAACT GTCTTGTTTT GTTACGAGGT GCTATGAAGT GGTGATGAAC 
GTAGTCCATC AATTGGCTGC CCTCTATATC AGTAACAAGA TTGCACCCAA AATTATAGAG 
ACAGCTGGAG TTCATTTTCA GACTATGTAT GAGCACTTGG GAGAGCTGCT AACAGTTTTG 
CTTACCCTGG ATGAAATTAT TGATAATCAT GTCACATTGA AAGAGCACTG GACTATGTAC 
AAAAGGTTAC TGAAATCTGT CCATCACAAT CCTTCAAAAT TTGGAATTCA GGAAGAAAAA 
CTAAAGCCAT TTGAAAAGTT CTTGCTGAAG CTAGAAGGGC AGTTACTTGA TGGAATGATA 
TTTCAGGCCT GTATAGAACA GCAGTTTGAT TCTCTCAATG GAGGAGTATC TGTGTCAAAA 
AATAGCACTT TTGCTGAAGA ATTTGCGCAT AGTATTCGCT CAATTTTTGC AAATGTAGAA 
GCCAAACTTG GAGAACCTTC TGAAATTGAC CAGAGAGACA AGTATGTTGG AATTTGTGGA 
CTTTTTGTAC TGCATTTTCA AATTTTTCGA ACTATTGATA AGAAGTTTTA TAAGTCTTTG 
TTGGACATTT GTAAGAAGGT ACCAGCCATC ACTCTAACTG CTAATATTAT TTGGTTTCCT 
GATAATTTTT TGATCCAGAA AATACCAGCA GCTGCCAAAC TTCTAGACAG AAAAAGTCTT 
CAAGCCATTA AAATACACAG AGATACTTTC CTACAGCAGA AAGCTCAATC ACTTACCAAA 
GATGTACAGT CTTACTACGT CTTTGTGAGC TCATGGATGA TGAAAATGGA ATCTATTTTA 
TCTAAAGAGC AGAGAATGGA TAAATTTGCT GAAGACCTCA CCAATAGATG TAATGTTTTT 
ATACAGGGCT TCTTATATGC ATACAGTATT AGTACCATTA TTAAAACCAC AATGAATCTC 
TACATGTCGA TGCAAAAGCC AATGACCAAA ACCTCAGTTA AGGCATTATG CAGGCTTGTT 
GAACTTCTCA AGGCAATAGA GCATATGTTC TACAGAAGAA GCATGGTTGT GGCTGATTCG 
GTCTCACATA TAACACAGCA CCTTCAACAT CAGGCTCTTA ATTCTATTTC TGTGGCAAAG 
AAAAGAGTAA TTTCTGACAA AAAATATAGC GAACAGCGTC TTGATGTGCT CTCTGCTCTA 
GTTTTGGCTG AAAACACTCT AAATGGACCA AGCACAAGGC AACGACGACT TATTGTTTCT 
TTGGCACTAA GTGTTGGCAC ACAAATGAAA ACATTTAAAG ATGAAGAACT CTTTCCACTG 
CAAGTAGTCA TGAAAAAGCT GGATCTTATC AGTGAGCTTA GAGAAAGAGT CCAAACACAG 
TGTGACTGTT GTTTTTTATA CTGGCACCGA GCTGTCTTCC CAATTTATTT AGATGATGTA 
TATGAAAATG CTGTTGATGC AGCCAGATTA CATTACATGT TCAGTGCTTT ACGGGACTGT 
GTACCTGCTA TGATGCATGC AAGGCATTTA GAGTCCTATG AAATACTTCT GGATTGCTAT 
GACAAAGAAA TTATGGAAAT TTTAAATGAG CATTTACTGG ACAAATTATG CAAGGAAATA 
GAGAAGGATC TGCGACTTTC TGTGCACACT CATTTAAAAC TGGATGACCG AAACCCTTTC 
AAAGTTGGCA TGAAAGACCT GGCCCTTTTT TTCTCTTTGA ATCCAATTCG ATTTTTCAAT 
CGTTTCATTG ACATTCGAGC TTATGTAACT CACTACCTAG ACAAGACTTT CTACAATCTA 
ACAACAGTGG CTCTTCATGA CTGGGCCACT TACAGCGAAA TGAGAAATTT AGCTACCCAG 
CGTTATGGAT TGGTTATGAC AGAGGCTCAT CTTCCTAGTC AGACTTTGGA ACAGGGCCTG 
GATGTTTTGG AAATTATGAG AAATATTCAT ATATTTGTGT CTCGATACCT CTATAATCTC 
AACAATCAGA TTTTTATTGA ACGAACAAGC AATAACAAAC ATTTGAACAC TATTAATATT 
CGGCATATTG CTAATTCAAT TCGAACACAT GGCACAGGAA TCATGAATAC AACAGTTAAT 
TTCACCTACC AGTTTTTGAA AAAGAAGTTC TATATATTCA GCCAATTTAT GTATGATGAA 
CATATCAAAT CTCGATTGAT TAAAGATATT CGGTTTTTCA GGGAAGTCAA GGACCAAAAT 
GATCATAAGT ATCCTTTTGA AAGAGCAGAA AAATTCAACC GTGGCATCAG AAAACTTGGA 
ATTACCCCAG AGGGACAAAG CTACCTTGAT CAGTTCAGGC AACTCATCAG CCAGATTGGT 
AACGCTATGG GCTATATACG GATGATAAGA TCTGGTGGCC TCCACTGTAG CAGCAACGCC 
ATCAGATTTG TGCCTGATCT TGAAGATATT GTAAATTTTG AAGAACTAGT AAAAGAAGAA 
GGTCTTGCAG AAGAAACATT AAAAGCAGCA AGGCATTTGG ATTCAGTCCT CAGTGATCAC 
ACACGAAATT CTGCTGAAGG CACGGAGTAT TTCAAAATGC TTGTAGATGT TTTTGCTCCA 
GAATTTCGAA GGCCAAAGAA TATACATCTT CGAAATTTCT ATATCATTGT TCCCCCTCTG 
ACCCTCAACT TTGTAGAGCA TTCCATTAGC TGCAAGGAGA AATTGAATAA AAAAAATAAA 
ATTGGAGCTG CTTTTACTGA TGATGGCTTT GCCATGGGTG TGGCTTACAT TTTGAAGCTT 
TTGGATCAGT ACCAGGAATT TGACTCACTT CACTGGTTCC AGTCTGTTAG AGAGAAGTAC 
CTGAAGGAGA TAAGAGCAGT TGCTAAGCAA CAGAATGTAC AGTCAGCTAG TCAAGATGAA 
AAACTGCTAC AAACCATGAA TCTCACTCAG AAGCGCTTGG ATGTCTGTCT ACAGGAATTT 
GAATTGCTCT ATTTCTCACT GAGCAGTGCA AGAATTTTCT TCAGAGCAGA CAAGACTGCG 
GCTGAAGAAA ACCAAGAAAA GAAAGAGAAA GAAGAGACAA AAACAAGCAA TGGAGACCTG 
TCCGACAGCA CTGTGTCTGC AGATCCTGTG GTCAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021543045.1
CDS	84..3602
Translation	MAVETLSPDWEFDRVDDGSQKIHAEVQLKNYGKFLEEYTSQLRRIEDALDDSIGDVWDFNLDPIALKLLPYEQSSLLELIKTENKVLNKVITVYAALCCEIKKLKYEAETKFYNGLLFYGEGATDSSMVEGDCQIQMGRFISFLQELSCFVTRCYEVVMNVVHQLAALYISNKIAPKIIETAGVHFQTMYEHLGELLTVLLTLDEIIDNHVTLKEHWTMYKRLLKSVHHNPSKFGIQEEKLKPFEKFLLKLEGQLLDGMIFQACIEQQFDSLNGGVSVSKNSTFAEEFAHSIRSIFANVEAKLGEPSEIDQRDKYVGICGLFVLHFQIFRTIDKKFYKSLLDICKKVPAITLTANIIWFPDNFLIQKIPAAAKLLDRKSLQAIKIHRDTFLQQKAQSLTKDVQSYYVFVSSWMMKMESILSKEQRMDKFAEDLTNRCNVFIQGFLYAYSISTIIKTTMNLYMSMQKPMTKTSVKALCRLVELLKAIEHMFYRRSMVVADSVSHITQHLQHQALNSISVAKKRVISDKKYSEQRLDVLSALVLAENTLNGPSTRQRRLIVSLALSVGTQMKTFKDEELFPLQVVMKKLDLISELRERVQTQCDCCFLYWHRAVFPIYLDDVYENAVDAARLHYMFSALRDCVPAMMHARHLESYEILLDCYDKEIMEILNEHLLDKLCKEIEKDLRLSVHTHLKLDDRNPFKVGMKDLALFFSLNPIRFFNRFIDIRAYVTHYLDKTFYNLTTVALHDWATYSEMRNLATQRYGLVMTEAHLPSQTLEQGLDVLEIMRNIHIFVSRYLYNLNNQIFIERTSNNKHLNTINIRHIANSIRTHGTGIMNTTVNFTYQFLKKKFYIFSQFMYDEHIKSRLIKDIRFFREVKDQNDHKYPFERAEKFNRGIRKLGITPEGQSYLDQFRQLISQIGNAMGYIRMIRSGGLHCSSNAIRFVPDLEDIVNFEELVKEEGLAEETLKAARHLDSVLSDHTRNSAEGTEYFKMLVDVFAPEFRRPKNIHLRNFYIIVPPLTLNFVEHSISCKEKLNKKNKIGAAFTDDGFAMGVAYILKLLDQYQEFDSLHWFQSVREKYLKEIRAVAKQQNVQSASQDEKLLQTMNLTQKRLDVCLQEFELLYFSLSSARIFFRADKTAAEENQEKKEKEETKTSNGDLSDSTVSADPVVK

Target ORF information:

RefSeq Version	XM_021687370.1
Organism	Neomonachus schauinslandi(Hawaiian monk seal)
Definition	Neomonachus schauinslandi WASH complex subunit 4 (WASHC4), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021687370.1

ORF Insert Sequence:

ATGGCGGTGG AGACTCTGTC CCCGGACTGG GAGTTCGACC GCGTTGACGA CGGCTCACAG 
AAAATTCATG CTGAAGTCCA ACTTAAGAAT TACGGGAAAT TTCTTGAGGA GTATACATCT 
CAATTGAGAA GAATTGAAGA TGCACTGGAT GATTCAATTG GTGATGTTTG GGATTTCAAT 
CTTGATCCAA TAGCATTAAA GCTTTTGCCT TATGAGCAGT CTTCCCTTTT GGAACTCATA 
AAGACTGAAA ACAAGGTCTT GAACAAAGTC ATCACTGTTT ATGCTGCACT TTGTTGTGAA 
ATCAAGAAAT TAAAGTATGA GGCTGAAACT AAATTTTATA ATGGTCTCTT GTTTTATGGA 
GAAGGAGCTA CAGATTCCAG CATGGTGGAA GGTGATTGCC AAATTCAAAT GGGAAGATTT 
ATTTCATTCT TACAGGAACT GTCTTGTTTT GTTACGAGGT GCTATGAAGT GGTGATGAAC 
GTAGTCCATC AATTGGCTGC CCTCTATATC AGTAACAAGA TTGCACCCAA AATTATAGAG 
ACAGCTGGAG TTCATTTTCA GACTATGTAT GAGCACTTGG GAGAGCTGCT AACAGTTTTG 
CTTACCCTGG ATGAAATTAT TGATAATCAT GTCACATTGA AAGAGCACTG GACTATGTAC 
AAAAGGTTAC TGAAATCTGT CCATCACAAT CCTTCAAAAT TTGGAATTCA GGAAGAAAAA 
CTAAAGCCAT TTGAAAAGTT CTTGCTGAAG CTAGAAGGGC AGTTACTTGA TGGAATGATA 
TTTCAGGCCT GTATAGAACA GCAGTTTGAT TCTCTCAATG GAGGAGTATC TGTGTCAAAA 
AATAGCACTT TTGCTGAAGA ATTTGCGCAT AGTATTCGCT CAATTTTTGC AAATGTAGAA 
GCCAAACTTG GAGAACCTTC TGAAATTGAC CAGAGAGACA AGTATGTTGG AATTTGTGGA 
CTTTTTGTAC TGCATTTTCA AATTTTTCGA ACTATTGATA AGAAGTTTTA TAAGTCTTTG 
TTGGACATTT GTAAGAAGGT ACCAGCCATC ACTCTAACTG CTAATATTAT TTGGTTTCCT 
GATAATTTTT TGATCCAGAA AATACCAGCA GCTGCCAAAC TTCTAGACAG AAAAAGTCTT 
CAAGCCATTA AAATACACAG AGATACTTTC CTACAGCAGA AAGCTCAATC ACTTACCAAA 
GATGTACAGT CTTACTACGT CTTTGTGAGC TCATGGATGA TGAAAATGGA ATCTATTTTA 
TCTAAAGAGC AGAGAATGGA TAAATTTGCT GAAGACCTCA CCAATAGATG TAATGTTTTT 
ATACAGGGCT TCTTATATGC ATACAGTATT AGTACCATTA TTAAAACCAC AATGAATCTC 
TACATGTCGA TGCAAAAGCC AATGACCAAA ACCTCAGTTA AGGCATTATG CAGGCTTGTT 
GAACTTCTCA AGGCAATAGA GCATATGTTC TACAGAAGAA GCATGGTTGT GGCTGATTCG 
GTCTCACATA TAACACAGCA CCTTCAACAT CAGGCTCTTA ATTCTATTTC TGTGGCAAAG 
AAAAGAGTAA TTTCTGACAA AAAATATAGC GAACAGCGTC TTGATGTGCT CTCTGCTCTA 
GTTTTGGCTG AAAACACTCT AAATGGACCA AGCACAAGGC AACGACGACT TATTGTTTCT 
TTGGCACTAA GTGTTGGCAC ACAAATGAAA ACATTTAAAG ATGAAGAACT CTTTCCACTG 
CAAGTAGTCA TGAAAAAGCT GGATCTTATC AGTGAGCTTA GAGAAAGAGT CCAAACACAG 
TGTGACTGTT GTTTTTTATA CTGGCACCGA GCTGTCTTCC CAATTTATTT AGATGATGTA 
TATGAAAATG CTGTTGATGC AGCCAGATTA CATTACATGT TCAGTGCTTT ACGGGACTGT 
GTACCTGCTA TGATGCATGC AAGGCATTTA GAGTCCTATG AAATACTTCT GGATTGCTAT 
GACAAAGAAA TTATGGAAAT TTTAAATGAG CATTTACTGG ACAAATTATG CAAGGAAATA 
GAGAAGGATC TGCGACTTTC TGTGCACACT CATTTAAAAC TGGATGACCG AAACCCTTTC 
AAAGTTGGCA TGAAAGACCT GGCCCTTTTT TTCTCTTTGA ATCCAATTCG ATTTTTCAAT 
CGTTTCATTG ACATTCGAGC TTATGTAACT CACTACCTAG ACAAGACTTT CTACAATCTA 
ACAACAGTGG CTCTTCATGA CTGGGCCACT TACAGCGAAA TGAGAAATTT AGCTACCCAG 
CGTTATGGAT TGGTTATGAC AGAGGCTCAT CTTCCTAGTC AGACTTTGGA ACAGGGCCTG 
GATGTTTTGG AAATTATGAG AAATATTCAT ATATTTGTGT CTCGATACCT CTATAATCTC 
AACAATCAGA TTTTTATTGA ACGAACAAGC AATAACAAAC ATTTGAACAC TATTAATATT 
CGGCATATTG CTAATTCAAT TCGAACACAT GGCACAGGAA TCATGAATAC AACAGTTAAT 
TTCACCTACC AGTTTTTGAA AAAGAAGTTC TATATATTCA GCCAATTTAT GTATGATGAA 
CATATCAAAT CTCGATTGAT TAAAGATATT CGGTTTTTCA GGGAAGTCAA GGACCAAAAT 
GATCATAAGT ATCCTTTTGA AAGAGCAGAA AAATTCAACC GTGGCATCAG AAAACTTGGA 
ATTACCCCAG AGGGACAAAG CTACCTTGAT CAGTTCAGGC AACTCATCAG CCAGATTGGT 
AACGCTATGG GCTATATACG GATGATAAGA TCTGGTGGCC TCCACTGTAG CAGCAACGCC 
ATCAGATTTG TGCCTGATCT TGAAGATATT GTAAATTTTG AAGAACTAGT AAAAGAAGAA 
GGTCTTGCAG AAGAAACATT AAAAGCAGCA AGGCATTTGG ATTCAGTCCT CAGTGATCAC 
ACACGAAATT CTGCTGAAGG CACGGAGTAT TTCAAAATGC TTGTAGATGT TTTTGCTCCA 
GAATTTCGAA GGCCAAAGAA TATACATCTT CGAAATTTCT ATATCATTGT TCCCCCTCTG 
ACCCTCAACT TTGTAGAGCA TTCCATTAGC TGCAAGGAGA AATTGAATAA AAAAAATAAA 
ATTGGAGCTG CTTTTACTGA TGATGGCTTT GCCATGGGTG TGGCTTACAT TTTGAAGCTT 
TTGGATCAGT ACCAGGAATT TGACTCACTT CACTGGTTCC AGTCTGTTAG AGAGAAGTAC 
CTGAAGGAGA TAAGAGCAGT TGCTAAGCAA CAGAATGTAC AGTCAGCTAG TCAAGATGAA 
AAACTGCTAC AAACCATGAA TCTCACTCAG AAGCGCTTGG ATGTCTGTCT ACAGGAATTT 
GAATTGCTCT ATTTCTCACT GAGCAGTGCA AGAATTTTCT TCAGAGCAGA CAAGACTGCG 
GCTGAAGAAA ACCAAGAAAA GAAAGAGAAA GAAGAGACAA AAACAAGCAA TGGAGACCTG 
TCCGACAGCA CTGTGTCTGC AGATCCTGTG GTCAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

WASHC4 ( XM_021687369.1 ) cDNA ORF clone, Neomonachus schauinslandi(Hawaiian monk seal) -> XP_021543044.1 Neomonachus schauinslandi WASH complex subunit 4 (WASHC4), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ONe140842
Clone ID Related Accession (Same CDS sequence)	XM_021687370.1 , XM_021687369.1
Accession Version	XM_021687369.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3519bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-27
Organism	Neomonachus schauinslandi(Hawaiian monk seal)
Product	WASH complex subunit 4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018734313.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Neomonachus schauinslandi Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGTGG AGACTCTGTC CCCGGACTGG GAGTTCGACC GCGTTGACGA CGGCTCACAG 
AAAATTCATG CTGAAGTCCA ACTTAAGAAT TACGGGAAAT TTCTTGAGGA GTATACATCT 
CAATTGAGAA GAATTGAAGA TGCACTGGAT GATTCAATTG GTGATGTTTG GGATTTCAAT 
CTTGATCCAA TAGCATTAAA GCTTTTGCCT TATGAGCAGT CTTCCCTTTT GGAACTCATA 
AAGACTGAAA ACAAGGTCTT GAACAAAGTC ATCACTGTTT ATGCTGCACT TTGTTGTGAA 
ATCAAGAAAT TAAAGTATGA GGCTGAAACT AAATTTTATA ATGGTCTCTT GTTTTATGGA 
GAAGGAGCTA CAGATTCCAG CATGGTGGAA GGTGATTGCC AAATTCAAAT GGGAAGATTT 
ATTTCATTCT TACAGGAACT GTCTTGTTTT GTTACGAGGT GCTATGAAGT GGTGATGAAC 
GTAGTCCATC AATTGGCTGC CCTCTATATC AGTAACAAGA TTGCACCCAA AATTATAGAG 
ACAGCTGGAG TTCATTTTCA GACTATGTAT GAGCACTTGG GAGAGCTGCT AACAGTTTTG 
CTTACCCTGG ATGAAATTAT TGATAATCAT GTCACATTGA AAGAGCACTG GACTATGTAC 
AAAAGGTTAC TGAAATCTGT CCATCACAAT CCTTCAAAAT TTGGAATTCA GGAAGAAAAA 
CTAAAGCCAT TTGAAAAGTT CTTGCTGAAG CTAGAAGGGC AGTTACTTGA TGGAATGATA 
TTTCAGGCCT GTATAGAACA GCAGTTTGAT TCTCTCAATG GAGGAGTATC TGTGTCAAAA 
AATAGCACTT TTGCTGAAGA ATTTGCGCAT AGTATTCGCT CAATTTTTGC AAATGTAGAA 
GCCAAACTTG GAGAACCTTC TGAAATTGAC CAGAGAGACA AGTATGTTGG AATTTGTGGA 
CTTTTTGTAC TGCATTTTCA AATTTTTCGA ACTATTGATA AGAAGTTTTA TAAGTCTTTG 
TTGGACATTT GTAAGAAGGT ACCAGCCATC ACTCTAACTG CTAATATTAT TTGGTTTCCT 
GATAATTTTT TGATCCAGAA AATACCAGCA GCTGCCAAAC TTCTAGACAG AAAAAGTCTT 
CAAGCCATTA AAATACACAG AGATACTTTC CTACAGCAGA AAGCTCAATC ACTTACCAAA 
GATGTACAGT CTTACTACGT CTTTGTGAGC TCATGGATGA TGAAAATGGA ATCTATTTTA 
TCTAAAGAGC AGAGAATGGA TAAATTTGCT GAAGACCTCA CCAATAGATG TAATGTTTTT 
ATACAGGGCT TCTTATATGC ATACAGTATT AGTACCATTA TTAAAACCAC AATGAATCTC 
TACATGTCGA TGCAAAAGCC AATGACCAAA ACCTCAGTTA AGGCATTATG CAGGCTTGTT 
GAACTTCTCA AGGCAATAGA GCATATGTTC TACAGAAGAA GCATGGTTGT GGCTGATTCG 
GTCTCACATA TAACACAGCA CCTTCAACAT CAGGCTCTTA ATTCTATTTC TGTGGCAAAG 
AAAAGAGTAA TTTCTGACAA AAAATATAGC GAACAGCGTC TTGATGTGCT CTCTGCTCTA 
GTTTTGGCTG AAAACACTCT AAATGGACCA AGCACAAGGC AACGACGACT TATTGTTTCT 
TTGGCACTAA GTGTTGGCAC ACAAATGAAA ACATTTAAAG ATGAAGAACT CTTTCCACTG 
CAAGTAGTCA TGAAAAAGCT GGATCTTATC AGTGAGCTTA GAGAAAGAGT CCAAACACAG 
TGTGACTGTT GTTTTTTATA CTGGCACCGA GCTGTCTTCC CAATTTATTT AGATGATGTA 
TATGAAAATG CTGTTGATGC AGCCAGATTA CATTACATGT TCAGTGCTTT ACGGGACTGT 
GTACCTGCTA TGATGCATGC AAGGCATTTA GAGTCCTATG AAATACTTCT GGATTGCTAT 
GACAAAGAAA TTATGGAAAT TTTAAATGAG CATTTACTGG ACAAATTATG CAAGGAAATA 
GAGAAGGATC TGCGACTTTC TGTGCACACT CATTTAAAAC TGGATGACCG AAACCCTTTC 
AAAGTTGGCA TGAAAGACCT GGCCCTTTTT TTCTCTTTGA ATCCAATTCG ATTTTTCAAT 
CGTTTCATTG ACATTCGAGC TTATGTAACT CACTACCTAG ACAAGACTTT CTACAATCTA 
ACAACAGTGG CTCTTCATGA CTGGGCCACT TACAGCGAAA TGAGAAATTT AGCTACCCAG 
CGTTATGGAT TGGTTATGAC AGAGGCTCAT CTTCCTAGTC AGACTTTGGA ACAGGGCCTG 
GATGTTTTGG AAATTATGAG AAATATTCAT ATATTTGTGT CTCGATACCT CTATAATCTC 
AACAATCAGA TTTTTATTGA ACGAACAAGC AATAACAAAC ATTTGAACAC TATTAATATT 
CGGCATATTG CTAATTCAAT TCGAACACAT GGCACAGGAA TCATGAATAC AACAGTTAAT 
TTCACCTACC AGTTTTTGAA AAAGAAGTTC TATATATTCA GCCAATTTAT GTATGATGAA 
CATATCAAAT CTCGATTGAT TAAAGATATT CGGTTTTTCA GGGAAGTCAA GGACCAAAAT 
GATCATAAGT ATCCTTTTGA AAGAGCAGAA AAATTCAACC GTGGCATCAG AAAACTTGGA 
ATTACCCCAG AGGGACAAAG CTACCTTGAT CAGTTCAGGC AACTCATCAG CCAGATTGGT 
AACGCTATGG GCTATATACG GATGATAAGA TCTGGTGGCC TCCACTGTAG CAGCAACGCC 
ATCAGATTTG TGCCTGATCT TGAAGATATT GTAAATTTTG AAGAACTAGT AAAAGAAGAA 
GGTCTTGCAG AAGAAACATT AAAAGCAGCA AGGCATTTGG ATTCAGTCCT CAGTGATCAC 
ACACGAAATT CTGCTGAAGG CACGGAGTAT TTCAAAATGC TTGTAGATGT TTTTGCTCCA 
GAATTTCGAA GGCCAAAGAA TATACATCTT CGAAATTTCT ATATCATTGT TCCCCCTCTG 
ACCCTCAACT TTGTAGAGCA TTCCATTAGC TGCAAGGAGA AATTGAATAA AAAAAATAAA 
ATTGGAGCTG CTTTTACTGA TGATGGCTTT GCCATGGGTG TGGCTTACAT TTTGAAGCTT 
TTGGATCAGT ACCAGGAATT TGACTCACTT CACTGGTTCC AGTCTGTTAG AGAGAAGTAC 
CTGAAGGAGA TAAGAGCAGT TGCTAAGCAA CAGAATGTAC AGTCAGCTAG TCAAGATGAA 
AAACTGCTAC AAACCATGAA TCTCACTCAG AAGCGCTTGG ATGTCTGTCT ACAGGAATTT 
GAATTGCTCT ATTTCTCACT GAGCAGTGCA AGAATTTTCT TCAGAGCAGA CAAGACTGCG 
GCTGAAGAAA ACCAAGAAAA GAAAGAGAAA GAAGAGACAA AAACAAGCAA TGGAGACCTG 
TCCGACAGCA CTGTGTCTGC AGATCCTGTG GTCAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021543044.1
CDS	84..3602
Translation	MAVETLSPDWEFDRVDDGSQKIHAEVQLKNYGKFLEEYTSQLRRIEDALDDSIGDVWDFNLDPIALKLLPYEQSSLLELIKTENKVLNKVITVYAALCCEIKKLKYEAETKFYNGLLFYGEGATDSSMVEGDCQIQMGRFISFLQELSCFVTRCYEVVMNVVHQLAALYISNKIAPKIIETAGVHFQTMYEHLGELLTVLLTLDEIIDNHVTLKEHWTMYKRLLKSVHHNPSKFGIQEEKLKPFEKFLLKLEGQLLDGMIFQACIEQQFDSLNGGVSVSKNSTFAEEFAHSIRSIFANVEAKLGEPSEIDQRDKYVGICGLFVLHFQIFRTIDKKFYKSLLDICKKVPAITLTANIIWFPDNFLIQKIPAAAKLLDRKSLQAIKIHRDTFLQQKAQSLTKDVQSYYVFVSSWMMKMESILSKEQRMDKFAEDLTNRCNVFIQGFLYAYSISTIIKTTMNLYMSMQKPMTKTSVKALCRLVELLKAIEHMFYRRSMVVADSVSHITQHLQHQALNSISVAKKRVISDKKYSEQRLDVLSALVLAENTLNGPSTRQRRLIVSLALSVGTQMKTFKDEELFPLQVVMKKLDLISELRERVQTQCDCCFLYWHRAVFPIYLDDVYENAVDAARLHYMFSALRDCVPAMMHARHLESYEILLDCYDKEIMEILNEHLLDKLCKEIEKDLRLSVHTHLKLDDRNPFKVGMKDLALFFSLNPIRFFNRFIDIRAYVTHYLDKTFYNLTTVALHDWATYSEMRNLATQRYGLVMTEAHLPSQTLEQGLDVLEIMRNIHIFVSRYLYNLNNQIFIERTSNNKHLNTINIRHIANSIRTHGTGIMNTTVNFTYQFLKKKFYIFSQFMYDEHIKSRLIKDIRFFREVKDQNDHKYPFERAEKFNRGIRKLGITPEGQSYLDQFRQLISQIGNAMGYIRMIRSGGLHCSSNAIRFVPDLEDIVNFEELVKEEGLAEETLKAARHLDSVLSDHTRNSAEGTEYFKMLVDVFAPEFRRPKNIHLRNFYIIVPPLTLNFVEHSISCKEKLNKKNKIGAAFTDDGFAMGVAYILKLLDQYQEFDSLHWFQSVREKYLKEIRAVAKQQNVQSASQDEKLLQTMNLTQKRLDVCLQEFELLYFSLSSARIFFRADKTAAEENQEKKEKEETKTSNGDLSDSTVSADPVVK

Target ORF information:

RefSeq Version	XM_021687369.1
Organism	Neomonachus schauinslandi(Hawaiian monk seal)
Definition	Neomonachus schauinslandi WASH complex subunit 4 (WASHC4), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021687369.1

ORF Insert Sequence:

ATGGCGGTGG AGACTCTGTC CCCGGACTGG GAGTTCGACC GCGTTGACGA CGGCTCACAG 
AAAATTCATG CTGAAGTCCA ACTTAAGAAT TACGGGAAAT TTCTTGAGGA GTATACATCT 
CAATTGAGAA GAATTGAAGA TGCACTGGAT GATTCAATTG GTGATGTTTG GGATTTCAAT 
CTTGATCCAA TAGCATTAAA GCTTTTGCCT TATGAGCAGT CTTCCCTTTT GGAACTCATA 
AAGACTGAAA ACAAGGTCTT GAACAAAGTC ATCACTGTTT ATGCTGCACT TTGTTGTGAA 
ATCAAGAAAT TAAAGTATGA GGCTGAAACT AAATTTTATA ATGGTCTCTT GTTTTATGGA 
GAAGGAGCTA CAGATTCCAG CATGGTGGAA GGTGATTGCC AAATTCAAAT GGGAAGATTT 
ATTTCATTCT TACAGGAACT GTCTTGTTTT GTTACGAGGT GCTATGAAGT GGTGATGAAC 
GTAGTCCATC AATTGGCTGC CCTCTATATC AGTAACAAGA TTGCACCCAA AATTATAGAG 
ACAGCTGGAG TTCATTTTCA GACTATGTAT GAGCACTTGG GAGAGCTGCT AACAGTTTTG 
CTTACCCTGG ATGAAATTAT TGATAATCAT GTCACATTGA AAGAGCACTG GACTATGTAC 
AAAAGGTTAC TGAAATCTGT CCATCACAAT CCTTCAAAAT TTGGAATTCA GGAAGAAAAA 
CTAAAGCCAT TTGAAAAGTT CTTGCTGAAG CTAGAAGGGC AGTTACTTGA TGGAATGATA 
TTTCAGGCCT GTATAGAACA GCAGTTTGAT TCTCTCAATG GAGGAGTATC TGTGTCAAAA 
AATAGCACTT TTGCTGAAGA ATTTGCGCAT AGTATTCGCT CAATTTTTGC AAATGTAGAA 
GCCAAACTTG GAGAACCTTC TGAAATTGAC CAGAGAGACA AGTATGTTGG AATTTGTGGA 
CTTTTTGTAC TGCATTTTCA AATTTTTCGA ACTATTGATA AGAAGTTTTA TAAGTCTTTG 
TTGGACATTT GTAAGAAGGT ACCAGCCATC ACTCTAACTG CTAATATTAT TTGGTTTCCT 
GATAATTTTT TGATCCAGAA AATACCAGCA GCTGCCAAAC TTCTAGACAG AAAAAGTCTT 
CAAGCCATTA AAATACACAG AGATACTTTC CTACAGCAGA AAGCTCAATC ACTTACCAAA 
GATGTACAGT CTTACTACGT CTTTGTGAGC TCATGGATGA TGAAAATGGA ATCTATTTTA 
TCTAAAGAGC AGAGAATGGA TAAATTTGCT GAAGACCTCA CCAATAGATG TAATGTTTTT 
ATACAGGGCT TCTTATATGC ATACAGTATT AGTACCATTA TTAAAACCAC AATGAATCTC 
TACATGTCGA TGCAAAAGCC AATGACCAAA ACCTCAGTTA AGGCATTATG CAGGCTTGTT 
GAACTTCTCA AGGCAATAGA GCATATGTTC TACAGAAGAA GCATGGTTGT GGCTGATTCG 
GTCTCACATA TAACACAGCA CCTTCAACAT CAGGCTCTTA ATTCTATTTC TGTGGCAAAG 
AAAAGAGTAA TTTCTGACAA AAAATATAGC GAACAGCGTC TTGATGTGCT CTCTGCTCTA 
GTTTTGGCTG AAAACACTCT AAATGGACCA AGCACAAGGC AACGACGACT TATTGTTTCT 
TTGGCACTAA GTGTTGGCAC ACAAATGAAA ACATTTAAAG ATGAAGAACT CTTTCCACTG 
CAAGTAGTCA TGAAAAAGCT GGATCTTATC AGTGAGCTTA GAGAAAGAGT CCAAACACAG 
TGTGACTGTT GTTTTTTATA CTGGCACCGA GCTGTCTTCC CAATTTATTT AGATGATGTA 
TATGAAAATG CTGTTGATGC AGCCAGATTA CATTACATGT TCAGTGCTTT ACGGGACTGT 
GTACCTGCTA TGATGCATGC AAGGCATTTA GAGTCCTATG AAATACTTCT GGATTGCTAT 
GACAAAGAAA TTATGGAAAT TTTAAATGAG CATTTACTGG ACAAATTATG CAAGGAAATA 
GAGAAGGATC TGCGACTTTC TGTGCACACT CATTTAAAAC TGGATGACCG AAACCCTTTC 
AAAGTTGGCA TGAAAGACCT GGCCCTTTTT TTCTCTTTGA ATCCAATTCG ATTTTTCAAT 
CGTTTCATTG ACATTCGAGC TTATGTAACT CACTACCTAG ACAAGACTTT CTACAATCTA 
ACAACAGTGG CTCTTCATGA CTGGGCCACT TACAGCGAAA TGAGAAATTT AGCTACCCAG 
CGTTATGGAT TGGTTATGAC AGAGGCTCAT CTTCCTAGTC AGACTTTGGA ACAGGGCCTG 
GATGTTTTGG AAATTATGAG AAATATTCAT ATATTTGTGT CTCGATACCT CTATAATCTC 
AACAATCAGA TTTTTATTGA ACGAACAAGC AATAACAAAC ATTTGAACAC TATTAATATT 
CGGCATATTG CTAATTCAAT TCGAACACAT GGCACAGGAA TCATGAATAC AACAGTTAAT 
TTCACCTACC AGTTTTTGAA AAAGAAGTTC TATATATTCA GCCAATTTAT GTATGATGAA 
CATATCAAAT CTCGATTGAT TAAAGATATT CGGTTTTTCA GGGAAGTCAA GGACCAAAAT 
GATCATAAGT ATCCTTTTGA AAGAGCAGAA AAATTCAACC GTGGCATCAG AAAACTTGGA 
ATTACCCCAG AGGGACAAAG CTACCTTGAT CAGTTCAGGC AACTCATCAG CCAGATTGGT 
AACGCTATGG GCTATATACG GATGATAAGA TCTGGTGGCC TCCACTGTAG CAGCAACGCC 
ATCAGATTTG TGCCTGATCT TGAAGATATT GTAAATTTTG AAGAACTAGT AAAAGAAGAA 
GGTCTTGCAG AAGAAACATT AAAAGCAGCA AGGCATTTGG ATTCAGTCCT CAGTGATCAC 
ACACGAAATT CTGCTGAAGG CACGGAGTAT TTCAAAATGC TTGTAGATGT TTTTGCTCCA 
GAATTTCGAA GGCCAAAGAA TATACATCTT CGAAATTTCT ATATCATTGT TCCCCCTCTG 
ACCCTCAACT TTGTAGAGCA TTCCATTAGC TGCAAGGAGA AATTGAATAA AAAAAATAAA 
ATTGGAGCTG CTTTTACTGA TGATGGCTTT GCCATGGGTG TGGCTTACAT TTTGAAGCTT 
TTGGATCAGT ACCAGGAATT TGACTCACTT CACTGGTTCC AGTCTGTTAG AGAGAAGTAC 
CTGAAGGAGA TAAGAGCAGT TGCTAAGCAA CAGAATGTAC AGTCAGCTAG TCAAGATGAA 
AAACTGCTAC AAACCATGAA TCTCACTCAG AAGCGCTTGG ATGTCTGTCT ACAGGAATTT 
GAATTGCTCT ATTTCTCACT GAGCAGTGCA AGAATTTTCT TCAGAGCAGA CAAGACTGCG 
GCTGAAGAAA ACCAAGAAAA GAAAGAGAAA GAAGAGACAA AAACAAGCAA TGGAGACCTG 
TCCGACAGCA CTGTGTCTGC AGATCCTGTG GTCAAATGA