Fcf1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Fcf1
Entrez Gene ID	73736
Full Name	FCF1 rRNA processing protein
Synonyms	1110008B24Rik
General protein information	Preferred Names FCF1 rRNA processing protein Names rRNA-processing protein FCF1 homolog FCF1 small subunit
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 12 Map Location: 12\|12 D1

mRNA and Protein(s)

mRNA	Protein	Name
NM_028632.2	NP_082908.2	rRNA-processing protein FCF1 homolog

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	FCF1	XP_003314465.1
Caenorhabditis elegans (roundworm)	F30A10.9	NP_492523.1
Kluyveromyces lactis	KLLA0A07018g	XP_451311.1
Oryza sativa (rice)	Os06g0158600	NP_001056867.1
Gallus gallus (chicken)	FCF1	XP_421270.1
Danio rerio (zebrafish)	fcf1	NP_001017601.1
Saccharomyces cerevisiae (baker's yeast)	FCF1	NP_010626.1
Arabidopsis thaliana (thale cress)	AT2G46230	NP_566068.1
Canis lupus familiaris (dog)	FCF1	XP_537508.2
Drosophila melanogaster (fruit fly)	Bka	NP_524867.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP009504	XP_310192.3
Eremothecium gossypii	AGOS_AEL102W	NP_984759.1
Schizosaccharomyces pombe (fission yeast)	SPBC32H8.04c	NP_595610.1
Magnaporthe oryzae (rice blast fungus)	MGG_08302	XP_003715775.1
Xenopus tropicalis (tropical clawed frog)	fcf1	NP_001004999.1
Neurospora crassa	NCU04159	XP_961315.1
Homo sapiens (human)	FCF1	NP_057046.1
	FCF1	XP_001088448.1
Bos taurus (cattle)	FCF1	NP_001032529.1
Mus musculus (house mouse)	Fcf1	NP_082908.2
Rattus norvegicus (Norway rat)	Fcf1	NP_001037700.1

Gene Ontology
Bibliography

Related articles in PubMed

Two orthogonal cleavages separate subunit RNAs in mouse ribosome biogenesis.
Wang M, Anikin L, Pestov DG
Nucleic acids research42(17)11180-91(2014)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Fcf1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Fcf1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu05894	NM_028632.2 Latest version!	Mus musculus FCF1 rRNA processing protein (Fcf1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Fcf1 ( NM_028632.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_082908.2 Mus musculus FCF1 rRNA processing protein (Fcf1), mRNA.

Price & Availability↑

CloneID	OMu05894
Clone ID Related Accession (Same CDS sequence)	NM_028632.2
Accession Version	NM_028632.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 597bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-22
Organism	Mus musculus(house mouse)
Product	rRNA-processing protein FCF1 homolog
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CJ085020.1 and BC094619.1. On Nov 18, 2009 this sequence version replaced NM_028632.1. ##Evidence-Data-START## Transcript exon combination :: BC094619.1, BU938561.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGGGAAGC AAAAGAAAAC AAGGAAATAT GCGACTATGA AGCGAATGCT CAGTCTGAGA 
GACGAGCGAC TTAAAGAAAA GGATAGGTTG AAGCCTAAGA AGAAAGAGAA GAAAGATCCG 
AGCGCGCTGA AGGAAAGAGA AGTCCCCCAG CATCCTTCCT GCTTGTTCTT CCAGTATAAT 
ACACAGCTGG GTCCACCTTA CCACATCCTT GTCGATACCA ACTTCATCAA CTTTTCCATT 
AAGGCCAAAC TGGACTTAGT GCAGTCAATG ATGGACTGTC TGTATGCCAA GTGTATCCCT 
TGTATAACTG ATTGTGTAAT GGCTGAAATT GAGAAATTGG GACAGAAGTT TCGTGTGGCA 
CTAAGAATCG CTAAGGATCC ACGGTTTGAC CGATTGCCGT GCACACACAA AGGAACCTAC 
GCTGACGACT GCTTGGTACA GAGAGTAACT CAGCACAAGT GTTACATTGT GGCCACAGTT 
GACCGTGACC TCAAACGAAG AATCCGGAAG ATCCCTGGCG TCCCTATCAT GTACTTATCT 
AACCACAGAT ACAACATCGA GCGGATGCCA GATGATTATG GAGCCCCTCG CTTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_082908.2
CDS	34..630
Translation	MGKQKKTRKYATMKRMLSLRDERLKEKDRLKPKKKEKKDPSALKEREVPQHPSCLFFQYNTQLGPPYHILVDTNFINFSIKAKLDLVQSMMDCLYAKCIPCITDCVMAEIEKLGQKFRVALRIAKDPRFDRLPCTHKGTYADDCLVQRVTQHKCYIVATVDRDLKRRIRKIPGVPIMYLSNHRYNIERMPDDYGAPRF

Target ORF information:

RefSeq Version	NM_028632.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus FCF1 rRNA processing protein (Fcf1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_028632.2

ORF Insert Sequence:

ATGGGGAAGC AAAAGAAAAC AAGGAAATAT GCGACTATGA AGCGAATGCT CAGTCTGAGA 
GACGAGCGAC TTAAAGAAAA GGATAGGTTG AAGCCTAAGA AGAAAGAGAA GAAAGATCCG 
AGCGCGCTGA AGGAAAGAGA AGTCCCCCAG CATCCTTCCT GCTTGTTCTT CCAGTATAAT 
ACACAGCTGG GTCCACCTTA CCACATCCTT GTCGATACCA ACTTCATCAA CTTTTCCATT 
AAGGCCAAAC TGGACTTAGT GCAGTCAATG ATGGACTGTC TGTATGCCAA GTGTATCCCT 
TGTATAACTG ATTGTGTAAT GGCTGAAATT GAGAAATTGG GACAGAAGTT TCGTGTGGCA 
CTAAGAATCG CTAAGGATCC ACGGTTTGAC CGATTGCCGT GCACACACAA AGGAACCTAC 
GCTGACGACT GCTTGGTACA GAGAGTAACT CAGCACAAGT GTTACATTGT GGCCACAGTT 
GACCGTGACC TCAAACGAAG AATCCGGAAG ATCCCTGGCG TCCCTATCAT GTACTTATCT 
AACCACAGAT ACAACATCGA GCGGATGCCA GATGATTATG GAGCCCCTCG CTTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Two orthogonal cleavages separate subunit RNAs in mouse ribosome biogenesis.
Nucleic acids research42(17)11180-91(2014)
Wang M,Anikin L,Pestov DG