Dppa2 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Dppa2
Entrez Gene ID	73703
Full Name	developmental pluripotency associated 2
Synonyms	2410088E07Rik,C80932,D19Mgi18,ECAT15-2
General protein information	Preferred Names developmental pluripotency associated 2 Names developmental pluripotency-associated protein 2 embryonic stem cell-associated transcript 15-2 protein pluripotent embryonic stem cell-related gene 1 protein
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 16 Map Location: 16\|16 B5

mRNA and Protein(s)

mRNA	Protein	Name
NM_028615.1	NP_082891.1	developmental pluripotency-associated protein 2

Pathways from BioSystems

Homologies

Homo sapiens (human)	DPPA2	NP_620170.3
Bos taurus (cattle)	DPPA2	NP_001193399.1
Rattus norvegicus (Norway rat)	LOC683282	XP_006248358.1
Pan troglodytes (chimpanzee)	DPPA2	XP_003309973.1
	DPPA2	XP_001096498.1
Mus musculus (house mouse)	Dppa2	NP_082891.1

Gene Ontology
Bibliography

Related articles in PubMed

LncRNA Dum interacts with Dnmts to regulate Dppa2 expression during myogenic differentiation and muscle regeneration.
Wang L, Zhao Y, Bao X, Zhu X, Kwok YK, Sun K, Chen X, Huang Y, Jauch R, Esteban MA, Sun H, Wang H
Cell research25(3)335-50(2015 Mar)

Identification of DPPA4 and DPPA2 as a novel family of pluripotency-related oncogenes.
Tung PY, Varlakhanova NV, Knoepfler PS
Stem cells (Dayton, Ohio)31(11)2330-42(2013 Nov)

Interaction between DMRT1 function and genetic background modulates signaling and pluripotency to control tumor susceptibility in the fetal germ line.
Krentz AD, Murphy MW, Zhang T, Sarver AL, Jain S, Griswold MD, Bardwell VJ, Zarkower D
Developmental biology377(1)67-78(2013 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

a novel long non-coding RNA was found to silence its neighboring gene, Dppa2, in cis through recruiting Dnmt1, Dnmt3a and Dnmt3b
Title: LncRNA Dum interacts with Dnmts to regulate Dppa2 expression during myogenic differentiation and muscle regeneration.

Results identified a new family of pluripotency-related oncogenes consisting of DPPA2 and DPPA4.
Title: Identification of DPPA4 and DPPA2 as a novel family of pluripotency-related oncogenes.

has important roles in lung development, where it is no longer expressed, by leaving epigenetic marks from earlier developmental stages
Title: Essential roles of ECAT15-2/Dppa2 in functional lung development.

Results showed that the expression of Dppa2 decreased markedly in differentiated cells. Dppa2 might play a role in maintenance of the undifferentiated state and proliferation of ES cells
Title: Dppa2 knockdown-induced differentiation and repressed proliferation of mouse embryonic stem cells.

Study of expression and function of pluripotent markers such as Dppa2 and Dppa4 is likely to unveil new aspects of the regulation of pluripotency and germ line development in mammals.
Title: Dppa2 and Dppa4 are closely linked SAP motif genes restricted to pluripotent cells and the germ line.

The following Dppa2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Dppa2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu05861	NM_028615.1 Latest version!	Mus musculus developmental pluripotency associated 2 (Dppa2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$188.30 $269.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Dppa2 ( NM_028615.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_082891.1 Mus musculus developmental pluripotency associated 2 (Dppa2), mRNA.

Price & Availability↑

CloneID	OMu05861
Clone ID Related Accession (Same CDS sequence)	NM_028615.1
Accession Version	NM_028615.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 906bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-07
Organism	Mus musculus(house mouse)
Product	developmental pluripotency-associated protein 2
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AY561246.1. On or before Jan 24, 2005 this sequence version replaced XM_358869.2, XM_129276.4, XM_489583.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AY561246.1, AK145645.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164134 [ECO:0000348] ##Evidence-Data-END##

ATGTCATACT TCGGCCTGGA GACTTTCAAC GAGAACCAAT CTGAGGAAAA CTTGGATGAA 
GAAAGTGTGA TTTTAACGCT GGTCCCCTTT AAGGAGGAGG AGGAGCCAAA CACAGACTAC 
GCTACGCAAT CAAATGTTTC TTCTTCAACC TTAGACCACA CACCACCAGC CCGTTCTCTG 
GTGAGGCATG CTGGAATCAA ACACCCAACC AGAACCATAC CAAGCACCTG TCCTCCGCCC 
AGCCTGCCTC CTATTAGGGA CGTGTCCCGG AACACTCTTC GGGAGTGGTG TCGGTATCAT 
AACTTGAGTA CGGATGGCAA GAAAGTTGAG GTCTATTTGA GACTTCGGAG ACACTCCTAT 
TCTAAACAAG AATGTTATAT TCCCAATACA TCTCGGGAGG CCAGAATGAA GCAGGGTCCC 
AAGAAATCCA AGATAGTCTT CAGAGGAATC GGGCCTCCAA GCGGGTGCCA AAGGAAAAAG 
GAGGAGAGTG GTGTCCTGGA AATTCTAACT TCACCTAAGG AGTCCACATT TGCAGCCTGG 
GCAAGGATTG CCATGAGAGC AGCTCAGTCA ATGTCTAAGA ATCGATGTCC TCTTCCGTCT 
AATGTGGAGG CCTTTCTGCC ACAAGCCACT GGATCCAGAT GGTGTGTTGT CCATGGCAGG 
CAGCTCCCTG CAGATAAGAA AGGTTGGGTT CGCCTGCAAT TTCTTGCTGG ACAGACCTGG 
GTTCCAGACA CTCCCCAAAG GATGAATTTT CTCTTCCTGT TACCGGCCTG TATTATCCCA 
GAACCAGGAG TGGAAGATAA TTTGCTATGT CCTGAATGTG TTCACAGCAA CAAGAAGATC 
CTAAGAAACT TTAAAATTAG AAGCCGTGCA AAGAAAAATG CCCTACCCCC AAATATGCCG 
CCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_082891.1
CDS	234..1139
Translation	MSYFGLETFNENQSEENLDEESVILTLVPFKEEEEPNTDYATQSNVSSSTLDHTPPARSLVRHAGIKHPTRTIPSTCPPPSLPPIRDVSRNTLREWCRYHNLSTDGKKVEVYLRLRRHSYSKQECYIPNTSREARMKQGPKKSKIVFRGIGPPSGCQRKKEESGVLEILTSPKESTFAAWARIAMRAAQSMSKNRCPLPSNVEAFLPQATGSRWCVVHGRQLPADKKGWVRLQFLAGQTWVPDTPQRMNFLFLLPACIIPEPGVEDNLLCPECVHSNKKILRNFKIRSRAKKNALPPNMPP

Target ORF information:

RefSeq Version	NM_028615.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus developmental pluripotency associated 2 (Dppa2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_028615.1

ORF Insert Sequence:

ATGTCATACT TCGGCCTGGA GACTTTCAAC GAGAACCAAT CTGAGGAAAA CTTGGATGAA 
GAAAGTGTGA TTTTAACGCT GGTCCCCTTT AAGGAGGAGG AGGAGCCAAA CACAGACTAC 
GCTACGCAAT CAAATGTTTC TTCTTCAACC TTAGACCACA CACCACCAGC CCGTTCTCTG 
GTGAGGCATG CTGGAATCAA ACACCCAACC AGAACCATAC CAAGCACCTG TCCTCCGCCC 
AGCCTGCCTC CTATTAGGGA CGTGTCCCGG AACACTCTTC GGGAGTGGTG TCGGTATCAT 
AACTTGAGTA CGGATGGCAA GAAAGTTGAG GTCTATTTGA GACTTCGGAG ACACTCCTAT 
TCTAAACAAG AATGTTATAT TCCCAATACA TCTCGGGAGG CCAGAATGAA GCAGGGTCCC 
AAGAAATCCA AGATAGTCTT CAGAGGAATC GGGCCTCCAA GCGGGTGCCA AAGGAAAAAG 
GAGGAGAGTG GTGTCCTGGA AATTCTAACT TCACCTAAGG AGTCCACATT TGCAGCCTGG 
GCAAGGATTG CCATGAGAGC AGCTCAGTCA ATGTCTAAGA ATCGATGTCC TCTTCCGTCT 
AATGTGGAGG CCTTTCTGCC ACAAGCCACT GGATCCAGAT GGTGTGTTGT CCATGGCAGG 
CAGCTCCCTG CAGATAAGAA AGGTTGGGTT CGCCTGCAAT TTCTTGCTGG ACAGACCTGG 
GTTCCAGACA CTCCCCAAAG GATGAATTTT CTCTTCCTGT TACCGGCCTG TATTATCCCA 
GAACCAGGAG TGGAAGATAA TTTGCTATGT CCTGAATGTG TTCACAGCAA CAAGAAGATC 
CTAAGAAACT TTAAAATTAG AAGCCGTGCA AAGAAAAATG CCCTACCCCC AAATATGCCG 
CCGTAG