Usp36 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Usp36
Entrez Gene ID	72344
Full Name	ubiquitin specific peptidase 36
Synonyms	2700002L06Rik,mKIAA1453
General protein information	Preferred Names ubiquitin specific peptidase 36 Names ubiquitin carboxyl-terminal hydrolase 36 deubiquitinating enzyme 36 ubiquitin specific protease 36 ubiquitin thioesterase 36 ubiquitin-specific-processing protease 36
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 11 Map Location: 11\|11 E2

mRNA and Protein(s)

mRNA	Protein	Name
XM_006534304.3	XP_006534367.1	ubiquitin carboxyl-terminal hydrolase 36 isoform X1
XM_011249273.2	XP_011247575.1	ubiquitin carboxyl-terminal hydrolase 36 isoform X2
NM_001033528.1	NP_001028700.1	ubiquitin carboxyl-terminal hydrolase 36
XM_006534304.4	XP_006534367.1	ubiquitin carboxyl-terminal hydrolase 36 isoform X1
XM_006534302.3	XP_006534365.1	ubiquitin carboxyl-terminal hydrolase 36 isoform X1
XM_006534302.4	XP_006534365.1	ubiquitin carboxyl-terminal hydrolase 36 isoform X1
XM_006534303.2	XP_006534366.1	ubiquitin carboxyl-terminal hydrolase 36 isoform X1

Pathways from BioSystems

Homologies

	USP36	XP_001108648.2
Gallus gallus (chicken)	USP36	XP_424101.3
Schizosaccharomyces pombe (fission yeast)	ubp16	NP_587805.1
Bos taurus (cattle)	USP36	XP_002696201.1
Canis lupus familiaris (dog)	USP36	XP_540463.3
Mus musculus (house mouse)	Usp36	NP_001028700.1
Pan troglodytes (chimpanzee)	USP36	XP_001146571.3
Homo sapiens (human)	USP36	NP_079366.3
Rattus norvegicus (Norway rat)	Usp36	NP_001100539.1

Gene Ontology
Bibliography

Related articles in PubMed

Loss of the Deubiquitinase USP36 Destabilizes the RNA Helicase DHX33 and Causes Preimplantation Lethality in Mice.
Fraile JM, Campos-Iglesias D, Rodr?guez F, Astudillo A, Vilarrasa-Blasi R, Verdaguer-Dot N, Prado MA, Paulo JA, Gygi SP, Mart?n-Subero JI, Freije JMP, L?pez-Ot?n C
The Journal of biological chemistry(2017 Dec)

High-throughput discovery of novel developmental phenotypes.
Dickinson ME, Flenniken AM, Ji X, Teboul L, Wong MD, White JK, Meehan TF, Weninger WJ, Westerberg H, Adissu H, Baker CN, Bower L, Brown JM, Caddle LB, Chiani F, Clary D, Cleak J, Daly MJ, Denegre JM, Doe B, Dolan ME, Edie SM, Fuchs H, Gailus-Durner V, Galli A, Gambadoro A, Gallegos J, Guo S, Horner NR, Hsu CW, Johnson SJ, Kalaga S, Keith LC, Lanoue L, Lawson TN, Lek M, Mark M, Marschall S, Mason J, McElwee ML, Newbigging S, Nutter LM, Peterson KA, Ramirez-Solis R, Rowland DJ, Ryder E, Samocha KE, Seavitt JR, Selloum M, Szoke-Kovacs Z, Tamura M, Trainor AG, Tudose I, Wakana S, Warren J, Wendling O, West DB, Wong L, Yoshiki A, , , , , , , , , MacArthur DG, Tocchini-Valentini GP, Gao X, Flicek P, Bradley A, Skarnes WC, Justice MJ, Parkinson HE, Moore M, Wells S, Braun RE, Svenson KL, de Angelis MH, Herault Y, Mohun T, Mallon AM, Henkelman RM, Brown SD, Adams DJ, Lloyd KC, McKerlie C, Beaudet AL, Bu?an M, Murray SA
Nature537(7621)508-514(2016 09)

Ubiquitin-specific Protease 36 (USP36) Controls Neuronal Precursor Cell-expressed Developmentally Down-regulated 4-2 (Nedd4-2) Actions over the Neurotrophin Receptor TrkA and Potassium Voltage-gated Channels 7.2/3 (Kv7.2/3).
Anta B, Mart?n-Rodr?guez C, Gomis-Perez C, Calvo L, L?pez-Benito S, Calder?n-Garc?a AA, Vicente-Garc?a C, Villarroel ?, Ar?valo JC
The Journal of biological chemistry291(36)19132-45(2016 09)

Prediction of the coding sequences of mouse homologues of KIAA gene: III. the complete nucleotide sequences of 500 mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries.
Okazaki N, Kikuno R, Ohara R, Inamoto S, Koseki H, Hiraoka S, Saga Y, Nagase T, Ohara O, Koga H
DNA research : an international journal for rapid publication of reports on genes and genomes10(4)167-80(2003 Aug)

BayGenomics: a resource of insertional mutations in mouse embryonic stem cells.
Stryke D, Kawamoto M, Huang CC, Johns SJ, King LA, Harper CA, Meng EC, Lee RE, Yee A, L'Italien L, Chuang PT, Young SG, Skarnes WC, Babbitt PC, Ferrin TE
Nucleic acids research31(1)278-81(2003 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

USP36 actions extend beyond TrkA because the presence of USP36 interferes with Nedd4-2-dependent Kv7.2/3 channel regulation.
Title: Ubiquitin-specific Protease 36 (USP36) Controls Neuronal Precursor Cell-expressed Developmentally Down-regulated 4-2 (Nedd4-2) Actions over the Neurotrophin Receptor TrkA and Potassium Voltage-gated Channels 7.2/3 (Kv7.2/3).

The following Usp36 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Usp36 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu70897	XM_011249273.2 Latest version!	Mus musculus ubiquitin specific peptidase 36 (Usp36), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OMu12714	XM_006534302.4 Latest version!	Mus musculus ubiquitin specific peptidase 36 (Usp36), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OMu12714	XM_006534304.4 Latest version!	Mus musculus ubiquitin specific peptidase 36 (Usp36), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OMu12714	NM_001033528.1 Latest version!	Mus musculus ubiquitin specific peptidase 36 (Usp36), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
OMu12714	XM_006534303.2 Latest version!	Mus musculus ubiquitin specific peptidase 36 (Usp36), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
View Old Accession Versions >>
OMu12714	XM_006534304.3	Mus musculus ubiquitin specific peptidase 36 (Usp36), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
OMu12714	XM_006534302.3	Mus musculus ubiquitin specific peptidase 36 (Usp36), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Usp36 ( XM_011249273.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011247575.1 Mus musculus ubiquitin specific peptidase 36 (Usp36), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OMu70897
Clone ID Related Accession (Same CDS sequence)	XM_011249273.2
Accession Version	XM_011249273.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1878bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	ubiquitin carboxyl-terminal hydrolase 36 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_011249273.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGAGAA AGCTCCCAGC CCCTGAGGAG GTTGGCGTAC CTGTGTCCAG AAATGGCTCC 
CTTCCTGGCC TGAAGTTACA GAACGGATGT GCTCCTGCCA AGACACCTGC CGGGTCCCCG 
TCCCCAAGAC TCACGCCCAC GCCCACTCAT ATGCCCACCA TTCTGGATGA GCCCGGAAAG 
AAGGTGAAGA AGTCAGCCCC ACTGCAGTCT CTCACCACAT CTCCAACCAC TTCTCAGGGC 
TCTCCTGGGA CCGGCGAGTC CCGGAGCCAG CGGCCCGGCT CCTGGGCAAG CAGAGACACC 
ATCTTCTCTA CCTCCCCGAA GCTCCTGGCC AGAGCCATTA CTAACGGGCA CAGGCTGAAG 
GGCGAAGGCA GTGGTGTGGA CCTGGAGAAG GGGGATTCAA GCAGCTCCAG CCCCGAGCAC 
TCTGCCAGCA GTGACCCTGC CAAGGCCCCG CAGACCGCAG AGAGCAGAGC TGCGCATGCC 
TGTGATTCTC AGGGAACAAA CTGTCCCACC GCTGGCCATC CCAAAGCGCT GTTGAATGGA 
GTAGATGCCA AGATGGTGAA GTTGAAGTCC CCGGCCCTGA GCAGCACCAC AACCGAGCCC 
ACAAGCCTCA TGTCTCCTCC ACCAGCCAAA AAACTGGCCC TGTCAGCCAA GAAGGCCAGC 
ACCCTGCGGA GGGCGACCGG CAATGACATC GGTTCACCTT CCCCCTCAGC ATTCTGCGAC 
CTCACCTCCC CCATGAAAGC CACCCACCCC GTCGTTGCCT CCACTGGGCC TGTCAGTAAG 
ACCAGGACTG CTGCACCTGC CCCCCGACCG TCCACCCACC CCCACTCTGC ATCTCTGTCC 
AGTAGTAGTG CCAAGCCCTT AGGGACATCA GAGCCACAGA GCTGCCGCCC CTCTGCCTGG 
ACACCCCTGC CTCAGGTCAA TGGACACTTC ACGTCCCACT TACACCAACT GCCAGAGGCC 
AGCGAGGCCC TACACAGCCC CTCCAAAAAG AGGAAAAAGA CCCCTAATGG AGATCCCCAG 
AGACTGGGCA TCGACACGCT CCTCCCACAG TGCCTCAGGG GAGCACCTGC AGCAGCACGC 
AGGAAGAGAA AGAAGAGGTG CTCGGAGGGT GAGGGTGCCA CAGCTCCCAA GCAGGAAGGC 
CAATTTCAGG ACCAGTCTTG GAGTTCCGGG AGCCAGAAGG AAGAGGGTAC GCAGCCCCAG 
GTAAATGGCC ATCAAGTGAG TCACATTCTG GACAGTTACC ATGTGAGCAG CAGGAAAAGG 
AGGAAGAGGA AAAGATCGGA AGGACTCAGC CAGGAAGCCA CCCCGTCGCA GGACCTAATT 
CAGCACAGCT GCTCCCCTGT AGACCACAGT GAGCCTGAGG CCAGGACAGA GTTGCAGAAG 
AAGAAGAAGA AGAAAAGAAG GAAAAGAAAG CCCGAGCCAC AGCAGGATGA AGAGAGCAAG 
CACCCAGGAG ACCAGAGGAG CCCAAGGCCC AGTGTCACCC CAGTCCCTGC GTTGAGTGTG 
AATGGCCATC TTCCTAGTGA CTGCTTGGGG CTGGGACAAG CACCTCTCGT TACCTGGAAC 
AGAGATCAGG AACCCGATGT GGTCCAGGCG TTGCTTCAGG ACTCATCTGA TAAGGCTTAT 
GGGAAAAAAG TTTTGACCTG GGATGGCGAG CCTTCAGCCA TCAGTCAGGA CGCCATCAAA 
GACAGCAGAC TAGCCCGGAC CCAGACCGTG GTTGACGACT GGGATGAAGA GTTTGATCGA 
GGGAAGGAAA AGAAAATTAA AAAGTTCAAG AGAGAAAAGA AGAGAAACTT CAATGCCTTC 
CAGAAGCTTC AGAGTAGGCG CAACTTTTGG TCTGTGACGC ATCCTGCTAA GGTGGCCAGC 
CTCAGCTATC GCCGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011247575.1
CDS	952..2829
Translation	MMRKLPAPEEVGVPVSRNGSLPGLKLQNGCAPAKTPAGSPSPRLTPTPTHMPTILDEPGKKVKKSAPLQSLTTSPTTSQGSPGTGESRSQRPGSWASRDTIFSTSPKLLARAITNGHRLKGEGSGVDLEKGDSSSSSPEHSASSDPAKAPQTAESRAAHACDSQGTNCPTAGHPKALLNGVDAKMVKLKSPALSSTTTEPTSLMSPPPAKKLALSAKKASTLRRATGNDIGSPSPSAFCDLTSPMKATHPVVASTGPVSKTRTAAPAPRPSTHPHSASLSSSSAKPLGTSEPQSCRPSAWTPLPQVNGHFTSHLHQLPEASEALHSPSKKRKKTPNGDPQRLGIDTLLPQCLRGAPAAARRKRKKRCSEGEGATAPKQEGQFQDQSWSSGSQKEEGTQPQVNGHQVSHILDSYHVSSRKRRKRKRSEGLSQEATPSQDLIQHSCSPVDHSEPEARTELQKKKKKKRRKRKPEPQQDEESKHPGDQRSPRPSVTPVPALSVNGHLPSDCLGLGQAPLVTWNRDQEPDVVQALLQDSSDKAYGKKVLTWDGEPSAISQDAIKDSRLARTQTVVDDWDEEFDRGKEKKIKKFKREKKRNFNAFQKLQSRRNFWSVTHPAKVASLSYRR

Target ORF information:

RefSeq Version	XM_011249273.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus ubiquitin specific peptidase 36 (Usp36), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011249273.2

ORF Insert Sequence:

ATGATGAGAA AGCTCCCAGC CCCTGAGGAG GTTGGCGTAC CTGTGTCCAG AAATGGCTCC 
CTTCCTGGCC TGAAGTTACA GAACGGATGT GCTCCTGCCA AGACACCTGC CGGGTCCCCG 
TCCCCAAGAC TCACGCCCAC GCCCACTCAT ATGCCCACCA TTCTGGATGA GCCCGGAAAG 
AAGGTGAAGA AGTCAGCCCC ACTGCAGTCT CTCACCACAT CTCCAACCAC TTCTCAGGGC 
TCTCCTGGGA CCGGCGAGTC CCGGAGCCAG CGGCCCGGCT CCTGGGCAAG CAGAGACACC 
ATCTTCTCTA CCTCCCCGAA GCTCCTGGCC AGAGCCATTA CTAACGGGCA CAGGCTGAAG 
GGCGAAGGCA GTGGTGTGGA CCTGGAGAAG GGGGATTCAA GCAGCTCCAG CCCCGAGCAC 
TCTGCCAGCA GTGACCCTGC CAAGGCCCCG CAGACCGCAG AGAGCAGAGC TGCGCATGCC 
TGTGATTCTC AGGGAACAAA CTGTCCCACC GCTGGCCATC CCAAAGCGCT GTTGAATGGA 
GTAGATGCCA AGATGGTGAA GTTGAAGTCC CCGGCCCTGA GCAGCACCAC AACCGAGCCC 
ACAAGCCTCA TGTCTCCTCC ACCAGCCAAA AAACTGGCCC TGTCAGCCAA GAAGGCCAGC 
ACCCTGCGGA GGGCGACCGG CAATGACATC GGTTCACCTT CCCCCTCAGC ATTCTGCGAC 
CTCACCTCCC CCATGAAAGC CACCCACCCC GTCGTTGCCT CCACTGGGCC TGTCAGTAAG 
ACCAGGACTG CTGCACCTGC CCCCCGACCG TCCACCCACC CCCACTCTGC ATCTCTGTCC 
AGTAGTAGTG CCAAGCCCTT AGGGACATCA GAGCCACAGA GCTGCCGCCC CTCTGCCTGG 
ACACCCCTGC CTCAGGTCAA TGGACACTTC ACGTCCCACT TACACCAACT GCCAGAGGCC 
AGCGAGGCCC TACACAGCCC CTCCAAAAAG AGGAAAAAGA CCCCTAATGG AGATCCCCAG 
AGACTGGGCA TCGACACGCT CCTCCCACAG TGCCTCAGGG GAGCACCTGC AGCAGCACGC 
AGGAAGAGAA AGAAGAGGTG CTCGGAGGGT GAGGGTGCCA CAGCTCCCAA GCAGGAAGGC 
CAATTTCAGG ACCAGTCTTG GAGTTCCGGG AGCCAGAAGG AAGAGGGTAC GCAGCCCCAG 
GTAAATGGCC ATCAAGTGAG TCACATTCTG GACAGTTACC ATGTGAGCAG CAGGAAAAGG 
AGGAAGAGGA AAAGATCGGA AGGACTCAGC CAGGAAGCCA CCCCGTCGCA GGACCTAATT 
CAGCACAGCT GCTCCCCTGT AGACCACAGT GAGCCTGAGG CCAGGACAGA GTTGCAGAAG 
AAGAAGAAGA AGAAAAGAAG GAAAAGAAAG CCCGAGCCAC AGCAGGATGA AGAGAGCAAG 
CACCCAGGAG ACCAGAGGAG CCCAAGGCCC AGTGTCACCC CAGTCCCTGC GTTGAGTGTG 
AATGGCCATC TTCCTAGTGA CTGCTTGGGG CTGGGACAAG CACCTCTCGT TACCTGGAAC 
AGAGATCAGG AACCCGATGT GGTCCAGGCG TTGCTTCAGG ACTCATCTGA TAAGGCTTAT 
GGGAAAAAAG TTTTGACCTG GGATGGCGAG CCTTCAGCCA TCAGTCAGGA CGCCATCAAA 
GACAGCAGAC TAGCCCGGAC CCAGACCGTG GTTGACGACT GGGATGAAGA GTTTGATCGA 
GGGAAGGAAA AGAAAATTAA AAAGTTCAAG AGAGAAAAGA AGAGAAACTT CAATGCCTTC 
CAGAAGCTTC AGAGTAGGCG CAACTTTTGG TCTGTGACGC ATCCTGCTAA GGTGGCCAGC 
CTCAGCTATC GCCGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Usp36 ( XM_006534304.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006534367.1 Mus musculus ubiquitin specific peptidase 36 (Usp36), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OMu12714
Clone ID Related Accession (Same CDS sequence)	XM_006534304.3 , NM_001033528.1 , XM_006534304.4 , XM_006534302.3 , XM_006534302.4 , XM_006534303.2
Accession Version	XM_006534304.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3297bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	ubiquitin carboxyl-terminal hydrolase 36 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006534304.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTATAG TCGATAAGCT GAAGGAGGCT CTCAAGCCTG GCCGCAAGGA CTCGGCCGAG 
GATGGGGACC TGGGCAGGCT GCTGGCAGCC TCTGCCAAGA AGGTGCTTCT GCAGAGGATT 
GAGTTTGAGC CGGCCAGCAA GAGCTTCTCC TACCAGCTGG AGTCCTTGAA GAGCAAATAC 
GTACTGTTGA GCGCCAGGGC TGAGGGAGCC AGTCGCCACA GGAGCGGAGA TGAACTCCAG 
GCCAGGAAAC CAGGCACCGA GCGTGTGTCT GGAAGTGGAG GCGACGGAGT CCCTGCCCCG 
CAGAAAGTGC TCTTCCCTGT GGAGCGTCTG TCCCTGAGGT GGGAACGTGT TTTCCGAGTT 
GGCGCAGGGC TGCACAACCT GGGTAACACC TGCTTCCTCA ACTCCACCAT CCAGTGCTTG 
ACCTACACAC CACCTCTGGC CAACTACTTG CTCTCCAAGG AGCACGCACG GAGCTGTCAC 
CAAGGGGGCT TCTGCATGCT GTGTCTCATG CAAAACCACA TGGTCCAGGC CTTTGCCAAC 
AGCGGCAATG CTATCAAGCC CGTCTCCTTC ATCAGAGACC TGAAAAAGAT TGCCCGGCAC 
TTCCGGTTCG GGAACCAAGA GGATGCACAC GAATTCCTGC GGTACACCAT TGATGCGATG 
CAGAAGGCCT GCTTGAATGG CTACGCTAAG TTGGATCGGC AGACACAGGC TACTACCCTG 
GTGCATCAGA TCTTTGGAGG CTATCTCAGG TCCCGAGTGA AGTGCTCTGT GTGCAAGAGT 
GTCTCAGACA CATACGATCC CTACTTGGAC ATAGCACTGG AGATCCGGCA AGCTGCAAAT 
ATTGTGCGCG CTCTGGAACT TTTTGTGAAG TCAGATGTCC TGAGTGGAGA GAACGCCTAT 
ATGTGTGCTA AGTGTAAGAA GAAGGTTCCA GCCAGCAAGC GCTTCACCAT CCATAGAACA 
TCCAATGTCC TGACTCTGTC CCTCAAGCGC TTCGCCAACT TTAGTGGGGG GAAGATCACC 
AAGGATGTTG GCTATCCAGA GTTCCTGAAC ATCCGTCCAT ACATGTCGCA GAGCAGCGGT 
GATCCTGTCA TGTATGGGCT CTATGCTGTC CTGGTGCACT CAGGCTACAG CTGTCACGCT 
GGGCACTACT ACTGCTATGT GAAGGCCAGC AATGGACAGT GGTACCAGAT GAACGACTCC 
TTGGTCCATT CCAGCAATGT CAAGGTGGTT CTGAACCAGC AGGCCTACGT GCTCTTCTAT 
CTGCGGATTC CAGGCTCTAA GAAAAGTCCT GAGGGCCCTG TCTCCAGAGT GGGCGCCACT 
CTCCCTAGCC GTCCCAAAGT TGTTCCAGAG CACTCCAAGA AGAGCCCTGG CAATGGGGTT 
GTTCCCTCCC CGCTGATGGC AAAGCGACAA GACTCTGTAA TGATGAGAAA GCTCCCAGCC 
CCTGAGGAGG TTGGCGTACC TGTGTCCAGA AATGGCTCCC TTCCTGGCCT GAAGTTACAG 
AACGGATGTG CTCCTGCCAA GACACCTGCC GGGTCCCCGT CCCCAAGACT CACGCCCACG 
CCCACTCATA TGCCCACCAT TCTGGATGAG CCCGGAAAGA AGGTGAAGAA GTCAGCCCCA 
CTGCAGTCTC TCACCACATC TCCAACCACT TCTCAGGGCT CTCCTGGGAC CGGCGAGTCC 
CGGAGCCAGC GGCCCGGCTC CTGGGCAAGC AGAGACACCA TCTTCTCTAC CTCCCCGAAG 
CTCCTGGCCA GAGCCATTAC TAACGGGCAC AGGCTGAAGG GCGAAGGCAG TGGTGTGGAC 
CTGGAGAAGG GGGATTCAAG CAGCTCCAGC CCCGAGCACT CTGCCAGCAG TGACCCTGCC 
AAGGCCCCGC AGACCGCAGA GAGCAGAGCT GCGCATGCCT GTGATTCTCA GGGAACAAAC 
TGTCCCACCG CTGGCCATCC CAAAGCGCTG TTGAATGGAG TAGATGCCAA GATGGTGAAG 
TTGAAGTCCC CGGCCCTGAG CAGCACCACA ACCGAGCCCA CAAGCCTCAT GTCTCCTCCA 
CCAGCCAAAA AACTGGCCCT GTCAGCCAAG AAGGCCAGCA CCCTGCGGAG GGCGACCGGC 
AATGACATCG GTTCACCTTC CCCCTCAGCA TTCTGCGACC TCACCTCCCC CATGAAAGCC 
ACCCACCCCG TCGTTGCCTC CACTGGGCCT GTCAGTAAGA CCAGGACTGC TGCACCTGCC 
CCCCGACCGT CCACCCACCC CCACTCTGCA TCTCTGTCCA GTAGTAGTGC CAAGCCCTTA 
GGGACATCAG AGCCACAGAG CTGCCGCCCC TCTGCCTGGA CACCCCTGCC TCAGGTCAAT 
GGACACTTCA CGTCCCACTT ACACCAACTG CCAGAGGCCA GCGAGGCCCT ACACAGCCCC 
TCCAAAAAGA GGAAAAAGAC CCCTAATGGA GATCCCCAGA GACTGGGCAT CGACACGCTC 
CTCCCACAGT GCCTCAGGGG AGCACCTGCA GCAGCACGCA GGAAGAGAAA GAAGAGGTGC 
TCGGAGGGTG AGGGTGCCAC AGCTCCCAAG CAGGAAGGCC AATTTCAGGA CCAGTCTTGG 
AGTTCCGGGA GCCAGAAGGA AGAGGGTACG CAGCCCCAGG TAAATGGCCA TCAAGTGAGT 
CACATTCTGG ACAGTTACCA TGTGAGCAGC AGGAAAAGGA GGAAGAGGAA AAGATCGGAA 
GGACTCAGCC AGGAAGCCAC CCCGTCGCAG GACCTAATTC AGCACAGCTG CTCCCCTGTA 
GACCACAGTG AGCCTGAGGC CAGGACAGAG TTGCAGAAGA AGAAGAAGAA GAAAAGAAGG 
AAAAGAAAGC CCGAGCCACA GCAGGATGAA GAGAGCAAGC ACCCAGGAGA CCAGAGGAGC 
CCAAGGCCCA GTGTCACCCC AGTCCCTGCG TTGAGTGTGA ATGGCCATCT TCCTAGTGAC 
TGCTTGGGGC TGGGACAAGC ACCTCTCGTT ACCTGGAACA GAGATCAGGA ACCCGATGTG 
GTCCAGGCGT TGCTTCAGGA CTCATCTGAT AAGGCTTATG GGAAAAAAGT TTTGACCTGG 
GATGGCGAGC CTTCAGCCAT CAGTCAGGAC GCCATCAAAG ACAGCAGACT AGCCCGGACC 
CAGACCGTGG TTGACGACTG GGATGAAGAG TTTGATCGAG GGAAGGAAAA GAAAATTAAA 
AAGTTCAAGA GAGAAAAGAA GAGAAACTTC AATGCCTTCC AGAAGCTTCA GAGTAGGCGC 
AACTTTTGGT CTGTGACGCA TCCTGCTAAG GTGGCCAGCC TCAGCTATCG CCGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006534367.1
CDS	313..3609
Translation	MPIVDKLKEALKPGRKDSAEDGDLGRLLAASAKKVLLQRIEFEPASKSFSYQLESLKSKYVLLSARAEGASRHRSGDELQARKPGTERVSGSGGDGVPAPQKVLFPVERLSLRWERVFRVGAGLHNLGNTCFLNSTIQCLTYTPPLANYLLSKEHARSCHQGGFCMLCLMQNHMVQAFANSGNAIKPVSFIRDLKKIARHFRFGNQEDAHEFLRYTIDAMQKACLNGYAKLDRQTQATTLVHQIFGGYLRSRVKCSVCKSVSDTYDPYLDIALEIRQAANIVRALELFVKSDVLSGENAYMCAKCKKKVPASKRFTIHRTSNVLTLSLKRFANFSGGKITKDVGYPEFLNIRPYMSQSSGDPVMYGLYAVLVHSGYSCHAGHYYCYVKASNGQWYQMNDSLVHSSNVKVVLNQQAYVLFYLRIPGSKKSPEGPVSRVGATLPSRPKVVPEHSKKSPGNGVVPSPLMAKRQDSVMMRKLPAPEEVGVPVSRNGSLPGLKLQNGCAPAKTPAGSPSPRLTPTPTHMPTILDEPGKKVKKSAPLQSLTTSPTTSQGSPGTGESRSQRPGSWASRDTIFSTSPKLLARAITNGHRLKGEGSGVDLEKGDSSSSSPEHSASSDPAKAPQTAESRAAHACDSQGTNCPTAGHPKALLNGVDAKMVKLKSPALSSTTTEPTSLMSPPPAKKLALSAKKASTLRRATGNDIGSPSPSAFCDLTSPMKATHPVVASTGPVSKTRTAAPAPRPSTHPHSASLSSSSAKPLGTSEPQSCRPSAWTPLPQVNGHFTSHLHQLPEASEALHSPSKKRKKTPNGDPQRLGIDTLLPQCLRGAPAAARRKRKKRCSEGEGATAPKQEGQFQDQSWSSGSQKEEGTQPQVNGHQVSHILDSYHVSSRKRRKRKRSEGLSQEATPSQDLIQHSCSPVDHSEPEARTELQKKKKKKRRKRKPEPQQDEESKHPGDQRSPRPSVTPVPALSVNGHLPSDCLGLGQAPLVTWNRDQEPDVVQALLQDSSDKAYGKKVLTWDGEPSAISQDAIKDSRLARTQTVVDDWDEEFDRGKEKKIKKFKREKKRNFNAFQKLQSRRNFWSVTHPAKVASLSYRR

Target ORF information:

RefSeq Version	XM_006534304.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus ubiquitin specific peptidase 36 (Usp36), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006534304.4

ORF Insert Sequence:

ATGCCTATAG TCGATAAGCT GAAGGAGGCT CTCAAGCCTG GCCGCAAGGA CTCGGCCGAG 
GATGGGGACC TGGGCAGGCT GCTGGCAGCC TCTGCCAAGA AGGTGCTTCT GCAGAGGATT 
GAGTTTGAGC CGGCCAGCAA GAGCTTCTCC TACCAGCTGG AGTCCTTGAA GAGCAAATAC 
GTACTGTTGA GCGCCAGGGC TGAGGGAGCC AGTCGCCACA GGAGCGGAGA TGAACTCCAG 
GCCAGGAAAC CAGGCACCGA GCGTGTGTCT GGAAGTGGAG GCGACGGAGT CCCTGCCCCG 
CAGAAAGTGC TCTTCCCTGT GGAGCGTCTG TCCCTGAGGT GGGAACGTGT TTTCCGAGTT 
GGCGCAGGGC TGCACAACCT GGGTAACACC TGCTTCCTCA ACTCCACCAT CCAGTGCTTG 
ACCTACACAC CACCTCTGGC CAACTACTTG CTCTCCAAGG AGCACGCACG GAGCTGTCAC 
CAAGGGGGCT TCTGCATGCT GTGTCTCATG CAAAACCACA TGGTCCAGGC CTTTGCCAAC 
AGCGGCAATG CTATCAAGCC CGTCTCCTTC ATCAGAGACC TGAAAAAGAT TGCCCGGCAC 
TTCCGGTTCG GGAACCAAGA GGATGCACAC GAATTCCTGC GGTACACCAT TGATGCGATG 
CAGAAGGCCT GCTTGAATGG CTACGCTAAG TTGGATCGGC AGACACAGGC TACTACCCTG 
GTGCATCAGA TCTTTGGAGG CTATCTCAGG TCCCGAGTGA AGTGCTCTGT GTGCAAGAGT 
GTCTCAGACA CATACGATCC CTACTTGGAC ATAGCACTGG AGATCCGGCA AGCTGCAAAT 
ATTGTGCGCG CTCTGGAACT TTTTGTGAAG TCAGATGTCC TGAGTGGAGA GAACGCCTAT 
ATGTGTGCTA AGTGTAAGAA GAAGGTTCCA GCCAGCAAGC GCTTCACCAT CCATAGAACA 
TCCAATGTCC TGACTCTGTC CCTCAAGCGC TTCGCCAACT TTAGTGGGGG GAAGATCACC 
AAGGATGTTG GCTATCCAGA GTTCCTGAAC ATCCGTCCAT ACATGTCGCA GAGCAGCGGT 
GATCCTGTCA TGTATGGGCT CTATGCTGTC CTGGTGCACT CAGGCTACAG CTGTCACGCT 
GGGCACTACT ACTGCTATGT GAAGGCCAGC AATGGACAGT GGTACCAGAT GAACGACTCC 
TTGGTCCATT CCAGCAATGT CAAGGTGGTT CTGAACCAGC AGGCCTACGT GCTCTTCTAT 
CTGCGGATTC CAGGCTCTAA GAAAAGTCCT GAGGGCCCTG TCTCCAGAGT GGGCGCCACT 
CTCCCTAGCC GTCCCAAAGT TGTTCCAGAG CACTCCAAGA AGAGCCCTGG CAATGGGGTT 
GTTCCCTCCC CGCTGATGGC AAAGCGACAA GACTCTGTAA TGATGAGAAA GCTCCCAGCC 
CCTGAGGAGG TTGGCGTACC TGTGTCCAGA AATGGCTCCC TTCCTGGCCT GAAGTTACAG 
AACGGATGTG CTCCTGCCAA GACACCTGCC GGGTCCCCGT CCCCAAGACT CACGCCCACG 
CCCACTCATA TGCCCACCAT TCTGGATGAG CCCGGAAAGA AGGTGAAGAA GTCAGCCCCA 
CTGCAGTCTC TCACCACATC TCCAACCACT TCTCAGGGCT CTCCTGGGAC CGGCGAGTCC 
CGGAGCCAGC GGCCCGGCTC CTGGGCAAGC AGAGACACCA TCTTCTCTAC CTCCCCGAAG 
CTCCTGGCCA GAGCCATTAC TAACGGGCAC AGGCTGAAGG GCGAAGGCAG TGGTGTGGAC 
CTGGAGAAGG GGGATTCAAG CAGCTCCAGC CCCGAGCACT CTGCCAGCAG TGACCCTGCC 
AAGGCCCCGC AGACCGCAGA GAGCAGAGCT GCGCATGCCT GTGATTCTCA GGGAACAAAC 
TGTCCCACCG CTGGCCATCC CAAAGCGCTG TTGAATGGAG TAGATGCCAA GATGGTGAAG 
TTGAAGTCCC CGGCCCTGAG CAGCACCACA ACCGAGCCCA CAAGCCTCAT GTCTCCTCCA 
CCAGCCAAAA AACTGGCCCT GTCAGCCAAG AAGGCCAGCA CCCTGCGGAG GGCGACCGGC 
AATGACATCG GTTCACCTTC CCCCTCAGCA TTCTGCGACC TCACCTCCCC CATGAAAGCC 
ACCCACCCCG TCGTTGCCTC CACTGGGCCT GTCAGTAAGA CCAGGACTGC TGCACCTGCC 
CCCCGACCGT CCACCCACCC CCACTCTGCA TCTCTGTCCA GTAGTAGTGC CAAGCCCTTA 
GGGACATCAG AGCCACAGAG CTGCCGCCCC TCTGCCTGGA CACCCCTGCC TCAGGTCAAT 
GGACACTTCA CGTCCCACTT ACACCAACTG CCAGAGGCCA GCGAGGCCCT ACACAGCCCC 
TCCAAAAAGA GGAAAAAGAC CCCTAATGGA GATCCCCAGA GACTGGGCAT CGACACGCTC 
CTCCCACAGT GCCTCAGGGG AGCACCTGCA GCAGCACGCA GGAAGAGAAA GAAGAGGTGC 
TCGGAGGGTG AGGGTGCCAC AGCTCCCAAG CAGGAAGGCC AATTTCAGGA CCAGTCTTGG 
AGTTCCGGGA GCCAGAAGGA AGAGGGTACG CAGCCCCAGG TAAATGGCCA TCAAGTGAGT 
CACATTCTGG ACAGTTACCA TGTGAGCAGC AGGAAAAGGA GGAAGAGGAA AAGATCGGAA 
GGACTCAGCC AGGAAGCCAC CCCGTCGCAG GACCTAATTC AGCACAGCTG CTCCCCTGTA 
GACCACAGTG AGCCTGAGGC CAGGACAGAG TTGCAGAAGA AGAAGAAGAA GAAAAGAAGG 
AAAAGAAAGC CCGAGCCACA GCAGGATGAA GAGAGCAAGC ACCCAGGAGA CCAGAGGAGC 
CCAAGGCCCA GTGTCACCCC AGTCCCTGCG TTGAGTGTGA ATGGCCATCT TCCTAGTGAC 
TGCTTGGGGC TGGGACAAGC ACCTCTCGTT ACCTGGAACA GAGATCAGGA ACCCGATGTG 
GTCCAGGCGT TGCTTCAGGA CTCATCTGAT AAGGCTTATG GGAAAAAAGT TTTGACCTGG 
GATGGCGAGC CTTCAGCCAT CAGTCAGGAC GCCATCAAAG ACAGCAGACT AGCCCGGACC 
CAGACCGTGG TTGACGACTG GGATGAAGAG TTTGATCGAG GGAAGGAAAA GAAAATTAAA 
AAGTTCAAGA GAGAAAAGAA GAGAAACTTC AATGCCTTCC AGAAGCTTCA GAGTAGGCGC 
AACTTTTGGT CTGTGACGCA TCCTGCTAAG GTGGCCAGCC TCAGCTATCG CCGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Usp36 ( XM_006534302.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006534365.1 Mus musculus ubiquitin specific peptidase 36 (Usp36), transcript variant X1, mRNA.

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CloneID	OMu12714
Clone ID Related Accession (Same CDS sequence)	XM_006534304.3 , NM_001033528.1 , XM_006534304.4 , XM_006534302.3 , XM_006534302.4 , XM_006534303.2
Accession Version	XM_006534302.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3297bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	ubiquitin carboxyl-terminal hydrolase 36 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006534302.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTATAG TCGATAAGCT GAAGGAGGCT CTCAAGCCTG GCCGCAAGGA CTCGGCCGAG 
GATGGGGACC TGGGCAGGCT GCTGGCAGCC TCTGCCAAGA AGGTGCTTCT GCAGAGGATT 
GAGTTTGAGC CGGCCAGCAA GAGCTTCTCC TACCAGCTGG AGTCCTTGAA GAGCAAATAC 
GTACTGTTGA GCGCCAGGGC TGAGGGAGCC AGTCGCCACA GGAGCGGAGA TGAACTCCAG 
GCCAGGAAAC CAGGCACCGA GCGTGTGTCT GGAAGTGGAG GCGACGGAGT CCCTGCCCCG 
CAGAAAGTGC TCTTCCCTGT GGAGCGTCTG TCCCTGAGGT GGGAACGTGT TTTCCGAGTT 
GGCGCAGGGC TGCACAACCT GGGTAACACC TGCTTCCTCA ACTCCACCAT CCAGTGCTTG 
ACCTACACAC CACCTCTGGC CAACTACTTG CTCTCCAAGG AGCACGCACG GAGCTGTCAC 
CAAGGGGGCT TCTGCATGCT GTGTCTCATG CAAAACCACA TGGTCCAGGC CTTTGCCAAC 
AGCGGCAATG CTATCAAGCC CGTCTCCTTC ATCAGAGACC TGAAAAAGAT TGCCCGGCAC 
TTCCGGTTCG GGAACCAAGA GGATGCACAC GAATTCCTGC GGTACACCAT TGATGCGATG 
CAGAAGGCCT GCTTGAATGG CTACGCTAAG TTGGATCGGC AGACACAGGC TACTACCCTG 
GTGCATCAGA TCTTTGGAGG CTATCTCAGG TCCCGAGTGA AGTGCTCTGT GTGCAAGAGT 
GTCTCAGACA CATACGATCC CTACTTGGAC ATAGCACTGG AGATCCGGCA AGCTGCAAAT 
ATTGTGCGCG CTCTGGAACT TTTTGTGAAG TCAGATGTCC TGAGTGGAGA GAACGCCTAT 
ATGTGTGCTA AGTGTAAGAA GAAGGTTCCA GCCAGCAAGC GCTTCACCAT CCATAGAACA 
TCCAATGTCC TGACTCTGTC CCTCAAGCGC TTCGCCAACT TTAGTGGGGG GAAGATCACC 
AAGGATGTTG GCTATCCAGA GTTCCTGAAC ATCCGTCCAT ACATGTCGCA GAGCAGCGGT 
GATCCTGTCA TGTATGGGCT CTATGCTGTC CTGGTGCACT CAGGCTACAG CTGTCACGCT 
GGGCACTACT ACTGCTATGT GAAGGCCAGC AATGGACAGT GGTACCAGAT GAACGACTCC 
TTGGTCCATT CCAGCAATGT CAAGGTGGTT CTGAACCAGC AGGCCTACGT GCTCTTCTAT 
CTGCGGATTC CAGGCTCTAA GAAAAGTCCT GAGGGCCCTG TCTCCAGAGT GGGCGCCACT 
CTCCCTAGCC GTCCCAAAGT TGTTCCAGAG CACTCCAAGA AGAGCCCTGG CAATGGGGTT 
GTTCCCTCCC CGCTGATGGC AAAGCGACAA GACTCTGTAA TGATGAGAAA GCTCCCAGCC 
CCTGAGGAGG TTGGCGTACC TGTGTCCAGA AATGGCTCCC TTCCTGGCCT GAAGTTACAG 
AACGGATGTG CTCCTGCCAA GACACCTGCC GGGTCCCCGT CCCCAAGACT CACGCCCACG 
CCCACTCATA TGCCCACCAT TCTGGATGAG CCCGGAAAGA AGGTGAAGAA GTCAGCCCCA 
CTGCAGTCTC TCACCACATC TCCAACCACT TCTCAGGGCT CTCCTGGGAC CGGCGAGTCC 
CGGAGCCAGC GGCCCGGCTC CTGGGCAAGC AGAGACACCA TCTTCTCTAC CTCCCCGAAG 
CTCCTGGCCA GAGCCATTAC TAACGGGCAC AGGCTGAAGG GCGAAGGCAG TGGTGTGGAC 
CTGGAGAAGG GGGATTCAAG CAGCTCCAGC CCCGAGCACT CTGCCAGCAG TGACCCTGCC 
AAGGCCCCGC AGACCGCAGA GAGCAGAGCT GCGCATGCCT GTGATTCTCA GGGAACAAAC 
TGTCCCACCG CTGGCCATCC CAAAGCGCTG TTGAATGGAG TAGATGCCAA GATGGTGAAG 
TTGAAGTCCC CGGCCCTGAG CAGCACCACA ACCGAGCCCA CAAGCCTCAT GTCTCCTCCA 
CCAGCCAAAA AACTGGCCCT GTCAGCCAAG AAGGCCAGCA CCCTGCGGAG GGCGACCGGC 
AATGACATCG GTTCACCTTC CCCCTCAGCA TTCTGCGACC TCACCTCCCC CATGAAAGCC 
ACCCACCCCG TCGTTGCCTC CACTGGGCCT GTCAGTAAGA CCAGGACTGC TGCACCTGCC 
CCCCGACCGT CCACCCACCC CCACTCTGCA TCTCTGTCCA GTAGTAGTGC CAAGCCCTTA 
GGGACATCAG AGCCACAGAG CTGCCGCCCC TCTGCCTGGA CACCCCTGCC TCAGGTCAAT 
GGACACTTCA CGTCCCACTT ACACCAACTG CCAGAGGCCA GCGAGGCCCT ACACAGCCCC 
TCCAAAAAGA GGAAAAAGAC CCCTAATGGA GATCCCCAGA GACTGGGCAT CGACACGCTC 
CTCCCACAGT GCCTCAGGGG AGCACCTGCA GCAGCACGCA GGAAGAGAAA GAAGAGGTGC 
TCGGAGGGTG AGGGTGCCAC AGCTCCCAAG CAGGAAGGCC AATTTCAGGA CCAGTCTTGG 
AGTTCCGGGA GCCAGAAGGA AGAGGGTACG CAGCCCCAGG TAAATGGCCA TCAAGTGAGT 
CACATTCTGG ACAGTTACCA TGTGAGCAGC AGGAAAAGGA GGAAGAGGAA AAGATCGGAA 
GGACTCAGCC AGGAAGCCAC CCCGTCGCAG GACCTAATTC AGCACAGCTG CTCCCCTGTA 
GACCACAGTG AGCCTGAGGC CAGGACAGAG TTGCAGAAGA AGAAGAAGAA GAAAAGAAGG 
AAAAGAAAGC CCGAGCCACA GCAGGATGAA GAGAGCAAGC ACCCAGGAGA CCAGAGGAGC 
CCAAGGCCCA GTGTCACCCC AGTCCCTGCG TTGAGTGTGA ATGGCCATCT TCCTAGTGAC 
TGCTTGGGGC TGGGACAAGC ACCTCTCGTT ACCTGGAACA GAGATCAGGA ACCCGATGTG 
GTCCAGGCGT TGCTTCAGGA CTCATCTGAT AAGGCTTATG GGAAAAAAGT TTTGACCTGG 
GATGGCGAGC CTTCAGCCAT CAGTCAGGAC GCCATCAAAG ACAGCAGACT AGCCCGGACC 
CAGACCGTGG TTGACGACTG GGATGAAGAG TTTGATCGAG GGAAGGAAAA GAAAATTAAA 
AAGTTCAAGA GAGAAAAGAA GAGAAACTTC AATGCCTTCC AGAAGCTTCA GAGTAGGCGC 
AACTTTTGGT CTGTGACGCA TCCTGCTAAG GTGGCCAGCC TCAGCTATCG CCGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006534365.1
CDS	466..3762
Translation	MPIVDKLKEALKPGRKDSAEDGDLGRLLAASAKKVLLQRIEFEPASKSFSYQLESLKSKYVLLSARAEGASRHRSGDELQARKPGTERVSGSGGDGVPAPQKVLFPVERLSLRWERVFRVGAGLHNLGNTCFLNSTIQCLTYTPPLANYLLSKEHARSCHQGGFCMLCLMQNHMVQAFANSGNAIKPVSFIRDLKKIARHFRFGNQEDAHEFLRYTIDAMQKACLNGYAKLDRQTQATTLVHQIFGGYLRSRVKCSVCKSVSDTYDPYLDIALEIRQAANIVRALELFVKSDVLSGENAYMCAKCKKKVPASKRFTIHRTSNVLTLSLKRFANFSGGKITKDVGYPEFLNIRPYMSQSSGDPVMYGLYAVLVHSGYSCHAGHYYCYVKASNGQWYQMNDSLVHSSNVKVVLNQQAYVLFYLRIPGSKKSPEGPVSRVGATLPSRPKVVPEHSKKSPGNGVVPSPLMAKRQDSVMMRKLPAPEEVGVPVSRNGSLPGLKLQNGCAPAKTPAGSPSPRLTPTPTHMPTILDEPGKKVKKSAPLQSLTTSPTTSQGSPGTGESRSQRPGSWASRDTIFSTSPKLLARAITNGHRLKGEGSGVDLEKGDSSSSSPEHSASSDPAKAPQTAESRAAHACDSQGTNCPTAGHPKALLNGVDAKMVKLKSPALSSTTTEPTSLMSPPPAKKLALSAKKASTLRRATGNDIGSPSPSAFCDLTSPMKATHPVVASTGPVSKTRTAAPAPRPSTHPHSASLSSSSAKPLGTSEPQSCRPSAWTPLPQVNGHFTSHLHQLPEASEALHSPSKKRKKTPNGDPQRLGIDTLLPQCLRGAPAAARRKRKKRCSEGEGATAPKQEGQFQDQSWSSGSQKEEGTQPQVNGHQVSHILDSYHVSSRKRRKRKRSEGLSQEATPSQDLIQHSCSPVDHSEPEARTELQKKKKKKRRKRKPEPQQDEESKHPGDQRSPRPSVTPVPALSVNGHLPSDCLGLGQAPLVTWNRDQEPDVVQALLQDSSDKAYGKKVLTWDGEPSAISQDAIKDSRLARTQTVVDDWDEEFDRGKEKKIKKFKREKKRNFNAFQKLQSRRNFWSVTHPAKVASLSYRR

Target ORF information:

RefSeq Version	XM_006534302.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus ubiquitin specific peptidase 36 (Usp36), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006534302.4

ORF Insert Sequence:

ATGCCTATAG TCGATAAGCT GAAGGAGGCT CTCAAGCCTG GCCGCAAGGA CTCGGCCGAG 
GATGGGGACC TGGGCAGGCT GCTGGCAGCC TCTGCCAAGA AGGTGCTTCT GCAGAGGATT 
GAGTTTGAGC CGGCCAGCAA GAGCTTCTCC TACCAGCTGG AGTCCTTGAA GAGCAAATAC 
GTACTGTTGA GCGCCAGGGC TGAGGGAGCC AGTCGCCACA GGAGCGGAGA TGAACTCCAG 
GCCAGGAAAC CAGGCACCGA GCGTGTGTCT GGAAGTGGAG GCGACGGAGT CCCTGCCCCG 
CAGAAAGTGC TCTTCCCTGT GGAGCGTCTG TCCCTGAGGT GGGAACGTGT TTTCCGAGTT 
GGCGCAGGGC TGCACAACCT GGGTAACACC TGCTTCCTCA ACTCCACCAT CCAGTGCTTG 
ACCTACACAC CACCTCTGGC CAACTACTTG CTCTCCAAGG AGCACGCACG GAGCTGTCAC 
CAAGGGGGCT TCTGCATGCT GTGTCTCATG CAAAACCACA TGGTCCAGGC CTTTGCCAAC 
AGCGGCAATG CTATCAAGCC CGTCTCCTTC ATCAGAGACC TGAAAAAGAT TGCCCGGCAC 
TTCCGGTTCG GGAACCAAGA GGATGCACAC GAATTCCTGC GGTACACCAT TGATGCGATG 
CAGAAGGCCT GCTTGAATGG CTACGCTAAG TTGGATCGGC AGACACAGGC TACTACCCTG 
GTGCATCAGA TCTTTGGAGG CTATCTCAGG TCCCGAGTGA AGTGCTCTGT GTGCAAGAGT 
GTCTCAGACA CATACGATCC CTACTTGGAC ATAGCACTGG AGATCCGGCA AGCTGCAAAT 
ATTGTGCGCG CTCTGGAACT TTTTGTGAAG TCAGATGTCC TGAGTGGAGA GAACGCCTAT 
ATGTGTGCTA AGTGTAAGAA GAAGGTTCCA GCCAGCAAGC GCTTCACCAT CCATAGAACA 
TCCAATGTCC TGACTCTGTC CCTCAAGCGC TTCGCCAACT TTAGTGGGGG GAAGATCACC 
AAGGATGTTG GCTATCCAGA GTTCCTGAAC ATCCGTCCAT ACATGTCGCA GAGCAGCGGT 
GATCCTGTCA TGTATGGGCT CTATGCTGTC CTGGTGCACT CAGGCTACAG CTGTCACGCT 
GGGCACTACT ACTGCTATGT GAAGGCCAGC AATGGACAGT GGTACCAGAT GAACGACTCC 
TTGGTCCATT CCAGCAATGT CAAGGTGGTT CTGAACCAGC AGGCCTACGT GCTCTTCTAT 
CTGCGGATTC CAGGCTCTAA GAAAAGTCCT GAGGGCCCTG TCTCCAGAGT GGGCGCCACT 
CTCCCTAGCC GTCCCAAAGT TGTTCCAGAG CACTCCAAGA AGAGCCCTGG CAATGGGGTT 
GTTCCCTCCC CGCTGATGGC AAAGCGACAA GACTCTGTAA TGATGAGAAA GCTCCCAGCC 
CCTGAGGAGG TTGGCGTACC TGTGTCCAGA AATGGCTCCC TTCCTGGCCT GAAGTTACAG 
AACGGATGTG CTCCTGCCAA GACACCTGCC GGGTCCCCGT CCCCAAGACT CACGCCCACG 
CCCACTCATA TGCCCACCAT TCTGGATGAG CCCGGAAAGA AGGTGAAGAA GTCAGCCCCA 
CTGCAGTCTC TCACCACATC TCCAACCACT TCTCAGGGCT CTCCTGGGAC CGGCGAGTCC 
CGGAGCCAGC GGCCCGGCTC CTGGGCAAGC AGAGACACCA TCTTCTCTAC CTCCCCGAAG 
CTCCTGGCCA GAGCCATTAC TAACGGGCAC AGGCTGAAGG GCGAAGGCAG TGGTGTGGAC 
CTGGAGAAGG GGGATTCAAG CAGCTCCAGC CCCGAGCACT CTGCCAGCAG TGACCCTGCC 
AAGGCCCCGC AGACCGCAGA GAGCAGAGCT GCGCATGCCT GTGATTCTCA GGGAACAAAC 
TGTCCCACCG CTGGCCATCC CAAAGCGCTG TTGAATGGAG TAGATGCCAA GATGGTGAAG 
TTGAAGTCCC CGGCCCTGAG CAGCACCACA ACCGAGCCCA CAAGCCTCAT GTCTCCTCCA 
CCAGCCAAAA AACTGGCCCT GTCAGCCAAG AAGGCCAGCA CCCTGCGGAG GGCGACCGGC 
AATGACATCG GTTCACCTTC CCCCTCAGCA TTCTGCGACC TCACCTCCCC CATGAAAGCC 
ACCCACCCCG TCGTTGCCTC CACTGGGCCT GTCAGTAAGA CCAGGACTGC TGCACCTGCC 
CCCCGACCGT CCACCCACCC CCACTCTGCA TCTCTGTCCA GTAGTAGTGC CAAGCCCTTA 
GGGACATCAG AGCCACAGAG CTGCCGCCCC TCTGCCTGGA CACCCCTGCC TCAGGTCAAT 
GGACACTTCA CGTCCCACTT ACACCAACTG CCAGAGGCCA GCGAGGCCCT ACACAGCCCC 
TCCAAAAAGA GGAAAAAGAC CCCTAATGGA GATCCCCAGA GACTGGGCAT CGACACGCTC 
CTCCCACAGT GCCTCAGGGG AGCACCTGCA GCAGCACGCA GGAAGAGAAA GAAGAGGTGC 
TCGGAGGGTG AGGGTGCCAC AGCTCCCAAG CAGGAAGGCC AATTTCAGGA CCAGTCTTGG 
AGTTCCGGGA GCCAGAAGGA AGAGGGTACG CAGCCCCAGG TAAATGGCCA TCAAGTGAGT 
CACATTCTGG ACAGTTACCA TGTGAGCAGC AGGAAAAGGA GGAAGAGGAA AAGATCGGAA 
GGACTCAGCC AGGAAGCCAC CCCGTCGCAG GACCTAATTC AGCACAGCTG CTCCCCTGTA 
GACCACAGTG AGCCTGAGGC CAGGACAGAG TTGCAGAAGA AGAAGAAGAA GAAAAGAAGG 
AAAAGAAAGC CCGAGCCACA GCAGGATGAA GAGAGCAAGC ACCCAGGAGA CCAGAGGAGC 
CCAAGGCCCA GTGTCACCCC AGTCCCTGCG TTGAGTGTGA ATGGCCATCT TCCTAGTGAC 
TGCTTGGGGC TGGGACAAGC ACCTCTCGTT ACCTGGAACA GAGATCAGGA ACCCGATGTG 
GTCCAGGCGT TGCTTCAGGA CTCATCTGAT AAGGCTTATG GGAAAAAAGT TTTGACCTGG 
GATGGCGAGC CTTCAGCCAT CAGTCAGGAC GCCATCAAAG ACAGCAGACT AGCCCGGACC 
CAGACCGTGG TTGACGACTG GGATGAAGAG TTTGATCGAG GGAAGGAAAA GAAAATTAAA 
AAGTTCAAGA GAGAAAAGAA GAGAAACTTC AATGCCTTCC AGAAGCTTCA GAGTAGGCGC 
AACTTTTGGT CTGTGACGCA TCCTGCTAAG GTGGCCAGCC TCAGCTATCG CCGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Usp36 ( NM_001033528.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001028700.1 Mus musculus ubiquitin specific peptidase 36 (Usp36), mRNA.

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CloneID	OMu12714
Clone ID Related Accession (Same CDS sequence)	XM_006534304.3 , NM_001033528.1 , XM_006534304.4 , XM_006534302.3 , XM_006534302.4 , XM_006534303.2
Accession Version	NM_001033528.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3297bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-20
Organism	Mus musculus(house mouse)
Product	ubiquitin carboxyl-terminal hydrolase 36
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL591404.4. On or before Apr 10, 2009 this sequence version replaced XM_916680.2, XM_126772.6. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END##

ATGCCTATAG TCGATAAGCT GAAGGAGGCT CTCAAGCCTG GCCGCAAGGA CTCGGCCGAG 
GATGGGGACC TGGGCAGGCT GCTGGCAGCC TCTGCCAAGA AGGTGCTTCT GCAGAGGATT 
GAGTTTGAGC CGGCCAGCAA GAGCTTCTCC TACCAGCTGG AGTCCTTGAA GAGCAAATAC 
GTACTGTTGA GCGCCAGGGC TGAGGGAGCC AGTCGCCACA GGAGCGGAGA TGAACTCCAG 
GCCAGGAAAC CAGGCACCGA GCGTGTGTCT GGAAGTGGAG GCGACGGAGT CCCTGCCCCG 
CAGAAAGTGC TCTTCCCTGT GGAGCGTCTG TCCCTGAGGT GGGAACGTGT TTTCCGAGTT 
GGCGCAGGGC TGCACAACCT GGGTAACACC TGCTTCCTCA ACTCCACCAT CCAGTGCTTG 
ACCTACACAC CACCTCTGGC CAACTACTTG CTCTCCAAGG AGCACGCACG GAGCTGTCAC 
CAAGGGGGCT TCTGCATGCT GTGTCTCATG CAAAACCACA TGGTCCAGGC CTTTGCCAAC 
AGCGGCAATG CTATCAAGCC CGTCTCCTTC ATCAGAGACC TGAAAAAGAT TGCCCGGCAC 
TTCCGGTTCG GGAACCAAGA GGATGCACAC GAATTCCTGC GGTACACCAT TGATGCGATG 
CAGAAGGCCT GCTTGAATGG CTACGCTAAG TTGGATCGGC AGACACAGGC TACTACCCTG 
GTGCATCAGA TCTTTGGAGG CTATCTCAGG TCCCGAGTGA AGTGCTCTGT GTGCAAGAGT 
GTCTCAGACA CATACGATCC CTACTTGGAC ATAGCACTGG AGATCCGGCA AGCTGCAAAT 
ATTGTGCGCG CTCTGGAACT TTTTGTGAAG TCAGATGTCC TGAGTGGAGA GAACGCCTAT 
ATGTGTGCTA AGTGTAAGAA GAAGGTTCCA GCCAGCAAGC GCTTCACCAT CCATAGAACA 
TCCAATGTCC TGACTCTGTC CCTCAAGCGC TTCGCCAACT TTAGTGGGGG GAAGATCACC 
AAGGATGTTG GCTATCCAGA GTTCCTGAAC ATCCGTCCAT ACATGTCGCA GAGCAGCGGT 
GATCCTGTCA TGTATGGGCT CTATGCTGTC CTGGTGCACT CAGGCTACAG CTGTCACGCT 
GGGCACTACT ACTGCTATGT GAAGGCCAGC AATGGACAGT GGTACCAGAT GAACGACTCC 
TTGGTCCATT CCAGCAATGT CAAGGTGGTT CTGAACCAGC AGGCCTACGT GCTCTTCTAT 
CTGCGGATTC CAGGCTCTAA GAAAAGTCCT GAGGGCCCTG TCTCCAGAGT GGGCGCCACT 
CTCCCTAGCC GTCCCAAAGT TGTTCCAGAG CACTCCAAGA AGAGCCCTGG CAATGGGGTT 
GTTCCCTCCC CGCTGATGGC AAAGCGACAA GACTCTGTAA TGATGAGAAA GCTCCCAGCC 
CCTGAGGAGG TTGGCGTACC TGTGTCCAGA AATGGCTCCC TTCCTGGCCT GAAGTTACAG 
AACGGATGTG CTCCTGCCAA GACACCTGCC GGGTCCCCGT CCCCAAGACT CACGCCCACG 
CCCACTCATA TGCCCACCAT TCTGGATGAG CCCGGAAAGA AGGTGAAGAA GTCAGCCCCA 
CTGCAGTCTC TCACCACATC TCCAACCACT TCTCAGGGCT CTCCTGGGAC CGGCGAGTCC 
CGGAGCCAGC GGCCCGGCTC CTGGGCAAGC AGAGACACCA TCTTCTCTAC CTCCCCGAAG 
CTCCTGGCCA GAGCCATTAC TAACGGGCAC AGGCTGAAGG GCGAAGGCAG TGGTGTGGAC 
CTGGAGAAGG GGGATTCAAG CAGCTCCAGC CCCGAGCACT CTGCCAGCAG TGACCCTGCC 
AAGGCCCCGC AGACCGCAGA GAGCAGAGCT GCGCATGCCT GTGATTCTCA GGGAACAAAC 
TGTCCCACCG CTGGCCATCC CAAAGCGCTG TTGAATGGAG TAGATGCCAA GATGGTGAAG 
TTGAAGTCCC CGGCCCTGAG CAGCACCACA ACCGAGCCCA CAAGCCTCAT GTCTCCTCCA 
CCAGCCAAAA AACTGGCCCT GTCAGCCAAG AAGGCCAGCA CCCTGCGGAG GGCGACCGGC 
AATGACATCG GTTCACCTTC CCCCTCAGCA TTCTGCGACC TCACCTCCCC CATGAAAGCC 
ACCCACCCCG TCGTTGCCTC CACTGGGCCT GTCAGTAAGA CCAGGACTGC TGCACCTGCC 
CCCCGACCGT CCACCCACCC CCACTCTGCA TCTCTGTCCA GTAGTAGTGC CAAGCCCTTA 
GGGACATCAG AGCCACAGAG CTGCCGCCCC TCTGCCTGGA CACCCCTGCC TCAGGTCAAT 
GGACACTTCA CGTCCCACTT ACACCAACTG CCAGAGGCCA GCGAGGCCCT ACACAGCCCC 
TCCAAAAAGA GGAAAAAGAC CCCTAATGGA GATCCCCAGA GACTGGGCAT CGACACGCTC 
CTCCCACAGT GCCTCAGGGG AGCACCTGCA GCAGCACGCA GGAAGAGAAA GAAGAGGTGC 
TCGGAGGGTG AGGGTGCCAC AGCTCCCAAG CAGGAAGGCC AATTTCAGGA CCAGTCTTGG 
AGTTCCGGGA GCCAGAAGGA AGAGGGTACG CAGCCCCAGG TAAATGGCCA TCAAGTGAGT 
CACATTCTGG ACAGTTACCA TGTGAGCAGC AGGAAAAGGA GGAAGAGGAA AAGATCGGAA 
GGACTCAGCC AGGAAGCCAC CCCGTCGCAG GACCTAATTC AGCACAGCTG CTCCCCTGTA 
GACCACAGTG AGCCTGAGGC CAGGACAGAG TTGCAGAAGA AGAAGAAGAA GAAAAGAAGG 
AAAAGAAAGC CCGAGCCACA GCAGGATGAA GAGAGCAAGC ACCCAGGAGA CCAGAGGAGC 
CCAAGGCCCA GTGTCACCCC AGTCCCTGCG TTGAGTGTGA ATGGCCATCT TCCTAGTGAC 
TGCTTGGGGC TGGGACAAGC ACCTCTCGTT ACCTGGAACA GAGATCAGGA ACCCGATGTG 
GTCCAGGCGT TGCTTCAGGA CTCATCTGAT AAGGCTTATG GGAAAAAAGT TTTGACCTGG 
GATGGCGAGC CTTCAGCCAT CAGTCAGGAC GCCATCAAAG ACAGCAGACT AGCCCGGACC 
CAGACCGTGG TTGACGACTG GGATGAAGAG TTTGATCGAG GGAAGGAAAA GAAAATTAAA 
AAGTTCAAGA GAGAAAAGAA GAGAAACTTC AATGCCTTCC AGAAGCTTCA GAGTAGGCGC 
AACTTTTGGT CTGTGACGCA TCCTGCTAAG GTGGCCAGCC TCAGCTATCG CCGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001028700.1
CDS	398..3694
Translation	MPIVDKLKEALKPGRKDSAEDGDLGRLLAASAKKVLLQRIEFEPASKSFSYQLESLKSKYVLLSARAEGASRHRSGDELQARKPGTERVSGSGGDGVPAPQKVLFPVERLSLRWERVFRVGAGLHNLGNTCFLNSTIQCLTYTPPLANYLLSKEHARSCHQGGFCMLCLMQNHMVQAFANSGNAIKPVSFIRDLKKIARHFRFGNQEDAHEFLRYTIDAMQKACLNGYAKLDRQTQATTLVHQIFGGYLRSRVKCSVCKSVSDTYDPYLDIALEIRQAANIVRALELFVKSDVLSGENAYMCAKCKKKVPASKRFTIHRTSNVLTLSLKRFANFSGGKITKDVGYPEFLNIRPYMSQSSGDPVMYGLYAVLVHSGYSCHAGHYYCYVKASNGQWYQMNDSLVHSSNVKVVLNQQAYVLFYLRIPGSKKSPEGPVSRVGATLPSRPKVVPEHSKKSPGNGVVPSPLMAKRQDSVMMRKLPAPEEVGVPVSRNGSLPGLKLQNGCAPAKTPAGSPSPRLTPTPTHMPTILDEPGKKVKKSAPLQSLTTSPTTSQGSPGTGESRSQRPGSWASRDTIFSTSPKLLARAITNGHRLKGEGSGVDLEKGDSSSSSPEHSASSDPAKAPQTAESRAAHACDSQGTNCPTAGHPKALLNGVDAKMVKLKSPALSSTTTEPTSLMSPPPAKKLALSAKKASTLRRATGNDIGSPSPSAFCDLTSPMKATHPVVASTGPVSKTRTAAPAPRPSTHPHSASLSSSSAKPLGTSEPQSCRPSAWTPLPQVNGHFTSHLHQLPEASEALHSPSKKRKKTPNGDPQRLGIDTLLPQCLRGAPAAARRKRKKRCSEGEGATAPKQEGQFQDQSWSSGSQKEEGTQPQVNGHQVSHILDSYHVSSRKRRKRKRSEGLSQEATPSQDLIQHSCSPVDHSEPEARTELQKKKKKKRRKRKPEPQQDEESKHPGDQRSPRPSVTPVPALSVNGHLPSDCLGLGQAPLVTWNRDQEPDVVQALLQDSSDKAYGKKVLTWDGEPSAISQDAIKDSRLARTQTVVDDWDEEFDRGKEKKIKKFKREKKRNFNAFQKLQSRRNFWSVTHPAKVASLSYRR

Target ORF information:

RefSeq Version	NM_001033528.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus ubiquitin specific peptidase 36 (Usp36), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001033528.1

ORF Insert Sequence:

ATGCCTATAG TCGATAAGCT GAAGGAGGCT CTCAAGCCTG GCCGCAAGGA CTCGGCCGAG 
GATGGGGACC TGGGCAGGCT GCTGGCAGCC TCTGCCAAGA AGGTGCTTCT GCAGAGGATT 
GAGTTTGAGC CGGCCAGCAA GAGCTTCTCC TACCAGCTGG AGTCCTTGAA GAGCAAATAC 
GTACTGTTGA GCGCCAGGGC TGAGGGAGCC AGTCGCCACA GGAGCGGAGA TGAACTCCAG 
GCCAGGAAAC CAGGCACCGA GCGTGTGTCT GGAAGTGGAG GCGACGGAGT CCCTGCCCCG 
CAGAAAGTGC TCTTCCCTGT GGAGCGTCTG TCCCTGAGGT GGGAACGTGT TTTCCGAGTT 
GGCGCAGGGC TGCACAACCT GGGTAACACC TGCTTCCTCA ACTCCACCAT CCAGTGCTTG 
ACCTACACAC CACCTCTGGC CAACTACTTG CTCTCCAAGG AGCACGCACG GAGCTGTCAC 
CAAGGGGGCT TCTGCATGCT GTGTCTCATG CAAAACCACA TGGTCCAGGC CTTTGCCAAC 
AGCGGCAATG CTATCAAGCC CGTCTCCTTC ATCAGAGACC TGAAAAAGAT TGCCCGGCAC 
TTCCGGTTCG GGAACCAAGA GGATGCACAC GAATTCCTGC GGTACACCAT TGATGCGATG 
CAGAAGGCCT GCTTGAATGG CTACGCTAAG TTGGATCGGC AGACACAGGC TACTACCCTG 
GTGCATCAGA TCTTTGGAGG CTATCTCAGG TCCCGAGTGA AGTGCTCTGT GTGCAAGAGT 
GTCTCAGACA CATACGATCC CTACTTGGAC ATAGCACTGG AGATCCGGCA AGCTGCAAAT 
ATTGTGCGCG CTCTGGAACT TTTTGTGAAG TCAGATGTCC TGAGTGGAGA GAACGCCTAT 
ATGTGTGCTA AGTGTAAGAA GAAGGTTCCA GCCAGCAAGC GCTTCACCAT CCATAGAACA 
TCCAATGTCC TGACTCTGTC CCTCAAGCGC TTCGCCAACT TTAGTGGGGG GAAGATCACC 
AAGGATGTTG GCTATCCAGA GTTCCTGAAC ATCCGTCCAT ACATGTCGCA GAGCAGCGGT 
GATCCTGTCA TGTATGGGCT CTATGCTGTC CTGGTGCACT CAGGCTACAG CTGTCACGCT 
GGGCACTACT ACTGCTATGT GAAGGCCAGC AATGGACAGT GGTACCAGAT GAACGACTCC 
TTGGTCCATT CCAGCAATGT CAAGGTGGTT CTGAACCAGC AGGCCTACGT GCTCTTCTAT 
CTGCGGATTC CAGGCTCTAA GAAAAGTCCT GAGGGCCCTG TCTCCAGAGT GGGCGCCACT 
CTCCCTAGCC GTCCCAAAGT TGTTCCAGAG CACTCCAAGA AGAGCCCTGG CAATGGGGTT 
GTTCCCTCCC CGCTGATGGC AAAGCGACAA GACTCTGTAA TGATGAGAAA GCTCCCAGCC 
CCTGAGGAGG TTGGCGTACC TGTGTCCAGA AATGGCTCCC TTCCTGGCCT GAAGTTACAG 
AACGGATGTG CTCCTGCCAA GACACCTGCC GGGTCCCCGT CCCCAAGACT CACGCCCACG 
CCCACTCATA TGCCCACCAT TCTGGATGAG CCCGGAAAGA AGGTGAAGAA GTCAGCCCCA 
CTGCAGTCTC TCACCACATC TCCAACCACT TCTCAGGGCT CTCCTGGGAC CGGCGAGTCC 
CGGAGCCAGC GGCCCGGCTC CTGGGCAAGC AGAGACACCA TCTTCTCTAC CTCCCCGAAG 
CTCCTGGCCA GAGCCATTAC TAACGGGCAC AGGCTGAAGG GCGAAGGCAG TGGTGTGGAC 
CTGGAGAAGG GGGATTCAAG CAGCTCCAGC CCCGAGCACT CTGCCAGCAG TGACCCTGCC 
AAGGCCCCGC AGACCGCAGA GAGCAGAGCT GCGCATGCCT GTGATTCTCA GGGAACAAAC 
TGTCCCACCG CTGGCCATCC CAAAGCGCTG TTGAATGGAG TAGATGCCAA GATGGTGAAG 
TTGAAGTCCC CGGCCCTGAG CAGCACCACA ACCGAGCCCA CAAGCCTCAT GTCTCCTCCA 
CCAGCCAAAA AACTGGCCCT GTCAGCCAAG AAGGCCAGCA CCCTGCGGAG GGCGACCGGC 
AATGACATCG GTTCACCTTC CCCCTCAGCA TTCTGCGACC TCACCTCCCC CATGAAAGCC 
ACCCACCCCG TCGTTGCCTC CACTGGGCCT GTCAGTAAGA CCAGGACTGC TGCACCTGCC 
CCCCGACCGT CCACCCACCC CCACTCTGCA TCTCTGTCCA GTAGTAGTGC CAAGCCCTTA 
GGGACATCAG AGCCACAGAG CTGCCGCCCC TCTGCCTGGA CACCCCTGCC TCAGGTCAAT 
GGACACTTCA CGTCCCACTT ACACCAACTG CCAGAGGCCA GCGAGGCCCT ACACAGCCCC 
TCCAAAAAGA GGAAAAAGAC CCCTAATGGA GATCCCCAGA GACTGGGCAT CGACACGCTC 
CTCCCACAGT GCCTCAGGGG AGCACCTGCA GCAGCACGCA GGAAGAGAAA GAAGAGGTGC 
TCGGAGGGTG AGGGTGCCAC AGCTCCCAAG CAGGAAGGCC AATTTCAGGA CCAGTCTTGG 
AGTTCCGGGA GCCAGAAGGA AGAGGGTACG CAGCCCCAGG TAAATGGCCA TCAAGTGAGT 
CACATTCTGG ACAGTTACCA TGTGAGCAGC AGGAAAAGGA GGAAGAGGAA AAGATCGGAA 
GGACTCAGCC AGGAAGCCAC CCCGTCGCAG GACCTAATTC AGCACAGCTG CTCCCCTGTA 
GACCACAGTG AGCCTGAGGC CAGGACAGAG TTGCAGAAGA AGAAGAAGAA GAAAAGAAGG 
AAAAGAAAGC CCGAGCCACA GCAGGATGAA GAGAGCAAGC ACCCAGGAGA CCAGAGGAGC 
CCAAGGCCCA GTGTCACCCC AGTCCCTGCG TTGAGTGTGA ATGGCCATCT TCCTAGTGAC 
TGCTTGGGGC TGGGACAAGC ACCTCTCGTT ACCTGGAACA GAGATCAGGA ACCCGATGTG 
GTCCAGGCGT TGCTTCAGGA CTCATCTGAT AAGGCTTATG GGAAAAAAGT TTTGACCTGG 
GATGGCGAGC CTTCAGCCAT CAGTCAGGAC GCCATCAAAG ACAGCAGACT AGCCCGGACC 
CAGACCGTGG TTGACGACTG GGATGAAGAG TTTGATCGAG GGAAGGAAAA GAAAATTAAA 
AAGTTCAAGA GAGAAAAGAA GAGAAACTTC AATGCCTTCC AGAAGCTTCA GAGTAGGCGC 
AACTTTTGGT CTGTGACGCA TCCTGCTAAG GTGGCCAGCC TCAGCTATCG CCGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Usp36 ( XM_006534304.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006534367.1 Mus musculus ubiquitin specific peptidase 36 (Usp36), transcript variant X3, mRNA.

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CloneID	OMu12714
Clone ID Related Accession (Same CDS sequence)	XM_006534304.3 , NM_001033528.1 , XM_006534304.4 , XM_006534302.3 , XM_006534302.4 , XM_006534303.2
Accession Version	XM_006534304.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3297bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	ubiquitin carboxyl-terminal hydrolase 36 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006534304.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTATAG TCGATAAGCT GAAGGAGGCT CTCAAGCCTG GCCGCAAGGA CTCGGCCGAG 
GATGGGGACC TGGGCAGGCT GCTGGCAGCC TCTGCCAAGA AGGTGCTTCT GCAGAGGATT 
GAGTTTGAGC CGGCCAGCAA GAGCTTCTCC TACCAGCTGG AGTCCTTGAA GAGCAAATAC 
GTACTGTTGA GCGCCAGGGC TGAGGGAGCC AGTCGCCACA GGAGCGGAGA TGAACTCCAG 
GCCAGGAAAC CAGGCACCGA GCGTGTGTCT GGAAGTGGAG GCGACGGAGT CCCTGCCCCG 
CAGAAAGTGC TCTTCCCTGT GGAGCGTCTG TCCCTGAGGT GGGAACGTGT TTTCCGAGTT 
GGCGCAGGGC TGCACAACCT GGGTAACACC TGCTTCCTCA ACTCCACCAT CCAGTGCTTG 
ACCTACACAC CACCTCTGGC CAACTACTTG CTCTCCAAGG AGCACGCACG GAGCTGTCAC 
CAAGGGGGCT TCTGCATGCT GTGTCTCATG CAAAACCACA TGGTCCAGGC CTTTGCCAAC 
AGCGGCAATG CTATCAAGCC CGTCTCCTTC ATCAGAGACC TGAAAAAGAT TGCCCGGCAC 
TTCCGGTTCG GGAACCAAGA GGATGCACAC GAATTCCTGC GGTACACCAT TGATGCGATG 
CAGAAGGCCT GCTTGAATGG CTACGCTAAG TTGGATCGGC AGACACAGGC TACTACCCTG 
GTGCATCAGA TCTTTGGAGG CTATCTCAGG TCCCGAGTGA AGTGCTCTGT GTGCAAGAGT 
GTCTCAGACA CATACGATCC CTACTTGGAC ATAGCACTGG AGATCCGGCA AGCTGCAAAT 
ATTGTGCGCG CTCTGGAACT TTTTGTGAAG TCAGATGTCC TGAGTGGAGA GAACGCCTAT 
ATGTGTGCTA AGTGTAAGAA GAAGGTTCCA GCCAGCAAGC GCTTCACCAT CCATAGAACA 
TCCAATGTCC TGACTCTGTC CCTCAAGCGC TTCGCCAACT TTAGTGGGGG GAAGATCACC 
AAGGATGTTG GCTATCCAGA GTTCCTGAAC ATCCGTCCAT ACATGTCGCA GAGCAGCGGT 
GATCCTGTCA TGTATGGGCT CTATGCTGTC CTGGTGCACT CAGGCTACAG CTGTCACGCT 
GGGCACTACT ACTGCTATGT GAAGGCCAGC AATGGACAGT GGTACCAGAT GAACGACTCC 
TTGGTCCATT CCAGCAATGT CAAGGTGGTT CTGAACCAGC AGGCCTACGT GCTCTTCTAT 
CTGCGGATTC CAGGCTCTAA GAAAAGTCCT GAGGGCCCTG TCTCCAGAGT GGGCGCCACT 
CTCCCTAGCC GTCCCAAAGT TGTTCCAGAG CACTCCAAGA AGAGCCCTGG CAATGGGGTT 
GTTCCCTCCC CGCTGATGGC AAAGCGACAA GACTCTGTAA TGATGAGAAA GCTCCCAGCC 
CCTGAGGAGG TTGGCGTACC TGTGTCCAGA AATGGCTCCC TTCCTGGCCT GAAGTTACAG 
AACGGATGTG CTCCTGCCAA GACACCTGCC GGGTCCCCGT CCCCAAGACT CACGCCCACG 
CCCACTCATA TGCCCACCAT TCTGGATGAG CCCGGAAAGA AGGTGAAGAA GTCAGCCCCA 
CTGCAGTCTC TCACCACATC TCCAACCACT TCTCAGGGCT CTCCTGGGAC CGGCGAGTCC 
CGGAGCCAGC GGCCCGGCTC CTGGGCAAGC AGAGACACCA TCTTCTCTAC CTCCCCGAAG 
CTCCTGGCCA GAGCCATTAC TAACGGGCAC AGGCTGAAGG GCGAAGGCAG TGGTGTGGAC 
CTGGAGAAGG GGGATTCAAG CAGCTCCAGC CCCGAGCACT CTGCCAGCAG TGACCCTGCC 
AAGGCCCCGC AGACCGCAGA GAGCAGAGCT GCGCATGCCT GTGATTCTCA GGGAACAAAC 
TGTCCCACCG CTGGCCATCC CAAAGCGCTG TTGAATGGAG TAGATGCCAA GATGGTGAAG 
TTGAAGTCCC CGGCCCTGAG CAGCACCACA ACCGAGCCCA CAAGCCTCAT GTCTCCTCCA 
CCAGCCAAAA AACTGGCCCT GTCAGCCAAG AAGGCCAGCA CCCTGCGGAG GGCGACCGGC 
AATGACATCG GTTCACCTTC CCCCTCAGCA TTCTGCGACC TCACCTCCCC CATGAAAGCC 
ACCCACCCCG TCGTTGCCTC CACTGGGCCT GTCAGTAAGA CCAGGACTGC TGCACCTGCC 
CCCCGACCGT CCACCCACCC CCACTCTGCA TCTCTGTCCA GTAGTAGTGC CAAGCCCTTA 
GGGACATCAG AGCCACAGAG CTGCCGCCCC TCTGCCTGGA CACCCCTGCC TCAGGTCAAT 
GGACACTTCA CGTCCCACTT ACACCAACTG CCAGAGGCCA GCGAGGCCCT ACACAGCCCC 
TCCAAAAAGA GGAAAAAGAC CCCTAATGGA GATCCCCAGA GACTGGGCAT CGACACGCTC 
CTCCCACAGT GCCTCAGGGG AGCACCTGCA GCAGCACGCA GGAAGAGAAA GAAGAGGTGC 
TCGGAGGGTG AGGGTGCCAC AGCTCCCAAG CAGGAAGGCC AATTTCAGGA CCAGTCTTGG 
AGTTCCGGGA GCCAGAAGGA AGAGGGTACG CAGCCCCAGG TAAATGGCCA TCAAGTGAGT 
CACATTCTGG ACAGTTACCA TGTGAGCAGC AGGAAAAGGA GGAAGAGGAA AAGATCGGAA 
GGACTCAGCC AGGAAGCCAC CCCGTCGCAG GACCTAATTC AGCACAGCTG CTCCCCTGTA 
GACCACAGTG AGCCTGAGGC CAGGACAGAG TTGCAGAAGA AGAAGAAGAA GAAAAGAAGG 
AAAAGAAAGC CCGAGCCACA GCAGGATGAA GAGAGCAAGC ACCCAGGAGA CCAGAGGAGC 
CCAAGGCCCA GTGTCACCCC AGTCCCTGCG TTGAGTGTGA ATGGCCATCT TCCTAGTGAC 
TGCTTGGGGC TGGGACAAGC ACCTCTCGTT ACCTGGAACA GAGATCAGGA ACCCGATGTG 
GTCCAGGCGT TGCTTCAGGA CTCATCTGAT AAGGCTTATG GGAAAAAAGT TTTGACCTGG 
GATGGCGAGC CTTCAGCCAT CAGTCAGGAC GCCATCAAAG ACAGCAGACT AGCCCGGACC 
CAGACCGTGG TTGACGACTG GGATGAAGAG TTTGATCGAG GGAAGGAAAA GAAAATTAAA 
AAGTTCAAGA GAGAAAAGAA GAGAAACTTC AATGCCTTCC AGAAGCTTCA GAGTAGGCGC 
AACTTTTGGT CTGTGACGCA TCCTGCTAAG GTGGCCAGCC TCAGCTATCG CCGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006534367.1
CDS	391..3687
Translation	MPIVDKLKEALKPGRKDSAEDGDLGRLLAASAKKVLLQRIEFEPASKSFSYQLESLKSKYVLLSARAEGASRHRSGDELQARKPGTERVSGSGGDGVPAPQKVLFPVERLSLRWERVFRVGAGLHNLGNTCFLNSTIQCLTYTPPLANYLLSKEHARSCHQGGFCMLCLMQNHMVQAFANSGNAIKPVSFIRDLKKIARHFRFGNQEDAHEFLRYTIDAMQKACLNGYAKLDRQTQATTLVHQIFGGYLRSRVKCSVCKSVSDTYDPYLDIALEIRQAANIVRALELFVKSDVLSGENAYMCAKCKKKVPASKRFTIHRTSNVLTLSLKRFANFSGGKITKDVGYPEFLNIRPYMSQSSGDPVMYGLYAVLVHSGYSCHAGHYYCYVKASNGQWYQMNDSLVHSSNVKVVLNQQAYVLFYLRIPGSKKSPEGPVSRVGATLPSRPKVVPEHSKKSPGNGVVPSPLMAKRQDSVMMRKLPAPEEVGVPVSRNGSLPGLKLQNGCAPAKTPAGSPSPRLTPTPTHMPTILDEPGKKVKKSAPLQSLTTSPTTSQGSPGTGESRSQRPGSWASRDTIFSTSPKLLARAITNGHRLKGEGSGVDLEKGDSSSSSPEHSASSDPAKAPQTAESRAAHACDSQGTNCPTAGHPKALLNGVDAKMVKLKSPALSSTTTEPTSLMSPPPAKKLALSAKKASTLRRATGNDIGSPSPSAFCDLTSPMKATHPVVASTGPVSKTRTAAPAPRPSTHPHSASLSSSSAKPLGTSEPQSCRPSAWTPLPQVNGHFTSHLHQLPEASEALHSPSKKRKKTPNGDPQRLGIDTLLPQCLRGAPAAARRKRKKRCSEGEGATAPKQEGQFQDQSWSSGSQKEEGTQPQVNGHQVSHILDSYHVSSRKRRKRKRSEGLSQEATPSQDLIQHSCSPVDHSEPEARTELQKKKKKKRRKRKPEPQQDEESKHPGDQRSPRPSVTPVPALSVNGHLPSDCLGLGQAPLVTWNRDQEPDVVQALLQDSSDKAYGKKVLTWDGEPSAISQDAIKDSRLARTQTVVDDWDEEFDRGKEKKIKKFKREKKRNFNAFQKLQSRRNFWSVTHPAKVASLSYRR

Target ORF information:

RefSeq Version	XM_006534304.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus ubiquitin specific peptidase 36 (Usp36), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006534304.3

ORF Insert Sequence:

ATGCCTATAG TCGATAAGCT GAAGGAGGCT CTCAAGCCTG GCCGCAAGGA CTCGGCCGAG 
GATGGGGACC TGGGCAGGCT GCTGGCAGCC TCTGCCAAGA AGGTGCTTCT GCAGAGGATT 
GAGTTTGAGC CGGCCAGCAA GAGCTTCTCC TACCAGCTGG AGTCCTTGAA GAGCAAATAC 
GTACTGTTGA GCGCCAGGGC TGAGGGAGCC AGTCGCCACA GGAGCGGAGA TGAACTCCAG 
GCCAGGAAAC CAGGCACCGA GCGTGTGTCT GGAAGTGGAG GCGACGGAGT CCCTGCCCCG 
CAGAAAGTGC TCTTCCCTGT GGAGCGTCTG TCCCTGAGGT GGGAACGTGT TTTCCGAGTT 
GGCGCAGGGC TGCACAACCT GGGTAACACC TGCTTCCTCA ACTCCACCAT CCAGTGCTTG 
ACCTACACAC CACCTCTGGC CAACTACTTG CTCTCCAAGG AGCACGCACG GAGCTGTCAC 
CAAGGGGGCT TCTGCATGCT GTGTCTCATG CAAAACCACA TGGTCCAGGC CTTTGCCAAC 
AGCGGCAATG CTATCAAGCC CGTCTCCTTC ATCAGAGACC TGAAAAAGAT TGCCCGGCAC 
TTCCGGTTCG GGAACCAAGA GGATGCACAC GAATTCCTGC GGTACACCAT TGATGCGATG 
CAGAAGGCCT GCTTGAATGG CTACGCTAAG TTGGATCGGC AGACACAGGC TACTACCCTG 
GTGCATCAGA TCTTTGGAGG CTATCTCAGG TCCCGAGTGA AGTGCTCTGT GTGCAAGAGT 
GTCTCAGACA CATACGATCC CTACTTGGAC ATAGCACTGG AGATCCGGCA AGCTGCAAAT 
ATTGTGCGCG CTCTGGAACT TTTTGTGAAG TCAGATGTCC TGAGTGGAGA GAACGCCTAT 
ATGTGTGCTA AGTGTAAGAA GAAGGTTCCA GCCAGCAAGC GCTTCACCAT CCATAGAACA 
TCCAATGTCC TGACTCTGTC CCTCAAGCGC TTCGCCAACT TTAGTGGGGG GAAGATCACC 
AAGGATGTTG GCTATCCAGA GTTCCTGAAC ATCCGTCCAT ACATGTCGCA GAGCAGCGGT 
GATCCTGTCA TGTATGGGCT CTATGCTGTC CTGGTGCACT CAGGCTACAG CTGTCACGCT 
GGGCACTACT ACTGCTATGT GAAGGCCAGC AATGGACAGT GGTACCAGAT GAACGACTCC 
TTGGTCCATT CCAGCAATGT CAAGGTGGTT CTGAACCAGC AGGCCTACGT GCTCTTCTAT 
CTGCGGATTC CAGGCTCTAA GAAAAGTCCT GAGGGCCCTG TCTCCAGAGT GGGCGCCACT 
CTCCCTAGCC GTCCCAAAGT TGTTCCAGAG CACTCCAAGA AGAGCCCTGG CAATGGGGTT 
GTTCCCTCCC CGCTGATGGC AAAGCGACAA GACTCTGTAA TGATGAGAAA GCTCCCAGCC 
CCTGAGGAGG TTGGCGTACC TGTGTCCAGA AATGGCTCCC TTCCTGGCCT GAAGTTACAG 
AACGGATGTG CTCCTGCCAA GACACCTGCC GGGTCCCCGT CCCCAAGACT CACGCCCACG 
CCCACTCATA TGCCCACCAT TCTGGATGAG CCCGGAAAGA AGGTGAAGAA GTCAGCCCCA 
CTGCAGTCTC TCACCACATC TCCAACCACT TCTCAGGGCT CTCCTGGGAC CGGCGAGTCC 
CGGAGCCAGC GGCCCGGCTC CTGGGCAAGC AGAGACACCA TCTTCTCTAC CTCCCCGAAG 
CTCCTGGCCA GAGCCATTAC TAACGGGCAC AGGCTGAAGG GCGAAGGCAG TGGTGTGGAC 
CTGGAGAAGG GGGATTCAAG CAGCTCCAGC CCCGAGCACT CTGCCAGCAG TGACCCTGCC 
AAGGCCCCGC AGACCGCAGA GAGCAGAGCT GCGCATGCCT GTGATTCTCA GGGAACAAAC 
TGTCCCACCG CTGGCCATCC CAAAGCGCTG TTGAATGGAG TAGATGCCAA GATGGTGAAG 
TTGAAGTCCC CGGCCCTGAG CAGCACCACA ACCGAGCCCA CAAGCCTCAT GTCTCCTCCA 
CCAGCCAAAA AACTGGCCCT GTCAGCCAAG AAGGCCAGCA CCCTGCGGAG GGCGACCGGC 
AATGACATCG GTTCACCTTC CCCCTCAGCA TTCTGCGACC TCACCTCCCC CATGAAAGCC 
ACCCACCCCG TCGTTGCCTC CACTGGGCCT GTCAGTAAGA CCAGGACTGC TGCACCTGCC 
CCCCGACCGT CCACCCACCC CCACTCTGCA TCTCTGTCCA GTAGTAGTGC CAAGCCCTTA 
GGGACATCAG AGCCACAGAG CTGCCGCCCC TCTGCCTGGA CACCCCTGCC TCAGGTCAAT 
GGACACTTCA CGTCCCACTT ACACCAACTG CCAGAGGCCA GCGAGGCCCT ACACAGCCCC 
TCCAAAAAGA GGAAAAAGAC CCCTAATGGA GATCCCCAGA GACTGGGCAT CGACACGCTC 
CTCCCACAGT GCCTCAGGGG AGCACCTGCA GCAGCACGCA GGAAGAGAAA GAAGAGGTGC 
TCGGAGGGTG AGGGTGCCAC AGCTCCCAAG CAGGAAGGCC AATTTCAGGA CCAGTCTTGG 
AGTTCCGGGA GCCAGAAGGA AGAGGGTACG CAGCCCCAGG TAAATGGCCA TCAAGTGAGT 
CACATTCTGG ACAGTTACCA TGTGAGCAGC AGGAAAAGGA GGAAGAGGAA AAGATCGGAA 
GGACTCAGCC AGGAAGCCAC CCCGTCGCAG GACCTAATTC AGCACAGCTG CTCCCCTGTA 
GACCACAGTG AGCCTGAGGC CAGGACAGAG TTGCAGAAGA AGAAGAAGAA GAAAAGAAGG 
AAAAGAAAGC CCGAGCCACA GCAGGATGAA GAGAGCAAGC ACCCAGGAGA CCAGAGGAGC 
CCAAGGCCCA GTGTCACCCC AGTCCCTGCG TTGAGTGTGA ATGGCCATCT TCCTAGTGAC 
TGCTTGGGGC TGGGACAAGC ACCTCTCGTT ACCTGGAACA GAGATCAGGA ACCCGATGTG 
GTCCAGGCGT TGCTTCAGGA CTCATCTGAT AAGGCTTATG GGAAAAAAGT TTTGACCTGG 
GATGGCGAGC CTTCAGCCAT CAGTCAGGAC GCCATCAAAG ACAGCAGACT AGCCCGGACC 
CAGACCGTGG TTGACGACTG GGATGAAGAG TTTGATCGAG GGAAGGAAAA GAAAATTAAA 
AAGTTCAAGA GAGAAAAGAA GAGAAACTTC AATGCCTTCC AGAAGCTTCA GAGTAGGCGC 
AACTTTTGGT CTGTGACGCA TCCTGCTAAG GTGGCCAGCC TCAGCTATCG CCGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Usp36 ( XM_006534302.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006534365.1 Mus musculus ubiquitin specific peptidase 36 (Usp36), transcript variant X1, mRNA.

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CloneID	OMu12714
Clone ID Related Accession (Same CDS sequence)	XM_006534304.3 , NM_001033528.1 , XM_006534304.4 , XM_006534302.3 , XM_006534302.4 , XM_006534303.2
Accession Version	XM_006534302.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3297bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	ubiquitin carboxyl-terminal hydrolase 36 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006534302.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTATAG TCGATAAGCT GAAGGAGGCT CTCAAGCCTG GCCGCAAGGA CTCGGCCGAG 
GATGGGGACC TGGGCAGGCT GCTGGCAGCC TCTGCCAAGA AGGTGCTTCT GCAGAGGATT 
GAGTTTGAGC CGGCCAGCAA GAGCTTCTCC TACCAGCTGG AGTCCTTGAA GAGCAAATAC 
GTACTGTTGA GCGCCAGGGC TGAGGGAGCC AGTCGCCACA GGAGCGGAGA TGAACTCCAG 
GCCAGGAAAC CAGGCACCGA GCGTGTGTCT GGAAGTGGAG GCGACGGAGT CCCTGCCCCG 
CAGAAAGTGC TCTTCCCTGT GGAGCGTCTG TCCCTGAGGT GGGAACGTGT TTTCCGAGTT 
GGCGCAGGGC TGCACAACCT GGGTAACACC TGCTTCCTCA ACTCCACCAT CCAGTGCTTG 
ACCTACACAC CACCTCTGGC CAACTACTTG CTCTCCAAGG AGCACGCACG GAGCTGTCAC 
CAAGGGGGCT TCTGCATGCT GTGTCTCATG CAAAACCACA TGGTCCAGGC CTTTGCCAAC 
AGCGGCAATG CTATCAAGCC CGTCTCCTTC ATCAGAGACC TGAAAAAGAT TGCCCGGCAC 
TTCCGGTTCG GGAACCAAGA GGATGCACAC GAATTCCTGC GGTACACCAT TGATGCGATG 
CAGAAGGCCT GCTTGAATGG CTACGCTAAG TTGGATCGGC AGACACAGGC TACTACCCTG 
GTGCATCAGA TCTTTGGAGG CTATCTCAGG TCCCGAGTGA AGTGCTCTGT GTGCAAGAGT 
GTCTCAGACA CATACGATCC CTACTTGGAC ATAGCACTGG AGATCCGGCA AGCTGCAAAT 
ATTGTGCGCG CTCTGGAACT TTTTGTGAAG TCAGATGTCC TGAGTGGAGA GAACGCCTAT 
ATGTGTGCTA AGTGTAAGAA GAAGGTTCCA GCCAGCAAGC GCTTCACCAT CCATAGAACA 
TCCAATGTCC TGACTCTGTC CCTCAAGCGC TTCGCCAACT TTAGTGGGGG GAAGATCACC 
AAGGATGTTG GCTATCCAGA GTTCCTGAAC ATCCGTCCAT ACATGTCGCA GAGCAGCGGT 
GATCCTGTCA TGTATGGGCT CTATGCTGTC CTGGTGCACT CAGGCTACAG CTGTCACGCT 
GGGCACTACT ACTGCTATGT GAAGGCCAGC AATGGACAGT GGTACCAGAT GAACGACTCC 
TTGGTCCATT CCAGCAATGT CAAGGTGGTT CTGAACCAGC AGGCCTACGT GCTCTTCTAT 
CTGCGGATTC CAGGCTCTAA GAAAAGTCCT GAGGGCCCTG TCTCCAGAGT GGGCGCCACT 
CTCCCTAGCC GTCCCAAAGT TGTTCCAGAG CACTCCAAGA AGAGCCCTGG CAATGGGGTT 
GTTCCCTCCC CGCTGATGGC AAAGCGACAA GACTCTGTAA TGATGAGAAA GCTCCCAGCC 
CCTGAGGAGG TTGGCGTACC TGTGTCCAGA AATGGCTCCC TTCCTGGCCT GAAGTTACAG 
AACGGATGTG CTCCTGCCAA GACACCTGCC GGGTCCCCGT CCCCAAGACT CACGCCCACG 
CCCACTCATA TGCCCACCAT TCTGGATGAG CCCGGAAAGA AGGTGAAGAA GTCAGCCCCA 
CTGCAGTCTC TCACCACATC TCCAACCACT TCTCAGGGCT CTCCTGGGAC CGGCGAGTCC 
CGGAGCCAGC GGCCCGGCTC CTGGGCAAGC AGAGACACCA TCTTCTCTAC CTCCCCGAAG 
CTCCTGGCCA GAGCCATTAC TAACGGGCAC AGGCTGAAGG GCGAAGGCAG TGGTGTGGAC 
CTGGAGAAGG GGGATTCAAG CAGCTCCAGC CCCGAGCACT CTGCCAGCAG TGACCCTGCC 
AAGGCCCCGC AGACCGCAGA GAGCAGAGCT GCGCATGCCT GTGATTCTCA GGGAACAAAC 
TGTCCCACCG CTGGCCATCC CAAAGCGCTG TTGAATGGAG TAGATGCCAA GATGGTGAAG 
TTGAAGTCCC CGGCCCTGAG CAGCACCACA ACCGAGCCCA CAAGCCTCAT GTCTCCTCCA 
CCAGCCAAAA AACTGGCCCT GTCAGCCAAG AAGGCCAGCA CCCTGCGGAG GGCGACCGGC 
AATGACATCG GTTCACCTTC CCCCTCAGCA TTCTGCGACC TCACCTCCCC CATGAAAGCC 
ACCCACCCCG TCGTTGCCTC CACTGGGCCT GTCAGTAAGA CCAGGACTGC TGCACCTGCC 
CCCCGACCGT CCACCCACCC CCACTCTGCA TCTCTGTCCA GTAGTAGTGC CAAGCCCTTA 
GGGACATCAG AGCCACAGAG CTGCCGCCCC TCTGCCTGGA CACCCCTGCC TCAGGTCAAT 
GGACACTTCA CGTCCCACTT ACACCAACTG CCAGAGGCCA GCGAGGCCCT ACACAGCCCC 
TCCAAAAAGA GGAAAAAGAC CCCTAATGGA GATCCCCAGA GACTGGGCAT CGACACGCTC 
CTCCCACAGT GCCTCAGGGG AGCACCTGCA GCAGCACGCA GGAAGAGAAA GAAGAGGTGC 
TCGGAGGGTG AGGGTGCCAC AGCTCCCAAG CAGGAAGGCC AATTTCAGGA CCAGTCTTGG 
AGTTCCGGGA GCCAGAAGGA AGAGGGTACG CAGCCCCAGG TAAATGGCCA TCAAGTGAGT 
CACATTCTGG ACAGTTACCA TGTGAGCAGC AGGAAAAGGA GGAAGAGGAA AAGATCGGAA 
GGACTCAGCC AGGAAGCCAC CCCGTCGCAG GACCTAATTC AGCACAGCTG CTCCCCTGTA 
GACCACAGTG AGCCTGAGGC CAGGACAGAG TTGCAGAAGA AGAAGAAGAA GAAAAGAAGG 
AAAAGAAAGC CCGAGCCACA GCAGGATGAA GAGAGCAAGC ACCCAGGAGA CCAGAGGAGC 
CCAAGGCCCA GTGTCACCCC AGTCCCTGCG TTGAGTGTGA ATGGCCATCT TCCTAGTGAC 
TGCTTGGGGC TGGGACAAGC ACCTCTCGTT ACCTGGAACA GAGATCAGGA ACCCGATGTG 
GTCCAGGCGT TGCTTCAGGA CTCATCTGAT AAGGCTTATG GGAAAAAAGT TTTGACCTGG 
GATGGCGAGC CTTCAGCCAT CAGTCAGGAC GCCATCAAAG ACAGCAGACT AGCCCGGACC 
CAGACCGTGG TTGACGACTG GGATGAAGAG TTTGATCGAG GGAAGGAAAA GAAAATTAAA 
AAGTTCAAGA GAGAAAAGAA GAGAAACTTC AATGCCTTCC AGAAGCTTCA GAGTAGGCGC 
AACTTTTGGT CTGTGACGCA TCCTGCTAAG GTGGCCAGCC TCAGCTATCG CCGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006534365.1
CDS	450..3746
Translation	MPIVDKLKEALKPGRKDSAEDGDLGRLLAASAKKVLLQRIEFEPASKSFSYQLESLKSKYVLLSARAEGASRHRSGDELQARKPGTERVSGSGGDGVPAPQKVLFPVERLSLRWERVFRVGAGLHNLGNTCFLNSTIQCLTYTPPLANYLLSKEHARSCHQGGFCMLCLMQNHMVQAFANSGNAIKPVSFIRDLKKIARHFRFGNQEDAHEFLRYTIDAMQKACLNGYAKLDRQTQATTLVHQIFGGYLRSRVKCSVCKSVSDTYDPYLDIALEIRQAANIVRALELFVKSDVLSGENAYMCAKCKKKVPASKRFTIHRTSNVLTLSLKRFANFSGGKITKDVGYPEFLNIRPYMSQSSGDPVMYGLYAVLVHSGYSCHAGHYYCYVKASNGQWYQMNDSLVHSSNVKVVLNQQAYVLFYLRIPGSKKSPEGPVSRVGATLPSRPKVVPEHSKKSPGNGVVPSPLMAKRQDSVMMRKLPAPEEVGVPVSRNGSLPGLKLQNGCAPAKTPAGSPSPRLTPTPTHMPTILDEPGKKVKKSAPLQSLTTSPTTSQGSPGTGESRSQRPGSWASRDTIFSTSPKLLARAITNGHRLKGEGSGVDLEKGDSSSSSPEHSASSDPAKAPQTAESRAAHACDSQGTNCPTAGHPKALLNGVDAKMVKLKSPALSSTTTEPTSLMSPPPAKKLALSAKKASTLRRATGNDIGSPSPSAFCDLTSPMKATHPVVASTGPVSKTRTAAPAPRPSTHPHSASLSSSSAKPLGTSEPQSCRPSAWTPLPQVNGHFTSHLHQLPEASEALHSPSKKRKKTPNGDPQRLGIDTLLPQCLRGAPAAARRKRKKRCSEGEGATAPKQEGQFQDQSWSSGSQKEEGTQPQVNGHQVSHILDSYHVSSRKRRKRKRSEGLSQEATPSQDLIQHSCSPVDHSEPEARTELQKKKKKKRRKRKPEPQQDEESKHPGDQRSPRPSVTPVPALSVNGHLPSDCLGLGQAPLVTWNRDQEPDVVQALLQDSSDKAYGKKVLTWDGEPSAISQDAIKDSRLARTQTVVDDWDEEFDRGKEKKIKKFKREKKRNFNAFQKLQSRRNFWSVTHPAKVASLSYRR

Target ORF information:

RefSeq Version	XM_006534302.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus ubiquitin specific peptidase 36 (Usp36), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006534302.3

ORF Insert Sequence:

ATGCCTATAG TCGATAAGCT GAAGGAGGCT CTCAAGCCTG GCCGCAAGGA CTCGGCCGAG 
GATGGGGACC TGGGCAGGCT GCTGGCAGCC TCTGCCAAGA AGGTGCTTCT GCAGAGGATT 
GAGTTTGAGC CGGCCAGCAA GAGCTTCTCC TACCAGCTGG AGTCCTTGAA GAGCAAATAC 
GTACTGTTGA GCGCCAGGGC TGAGGGAGCC AGTCGCCACA GGAGCGGAGA TGAACTCCAG 
GCCAGGAAAC CAGGCACCGA GCGTGTGTCT GGAAGTGGAG GCGACGGAGT CCCTGCCCCG 
CAGAAAGTGC TCTTCCCTGT GGAGCGTCTG TCCCTGAGGT GGGAACGTGT TTTCCGAGTT 
GGCGCAGGGC TGCACAACCT GGGTAACACC TGCTTCCTCA ACTCCACCAT CCAGTGCTTG 
ACCTACACAC CACCTCTGGC CAACTACTTG CTCTCCAAGG AGCACGCACG GAGCTGTCAC 
CAAGGGGGCT TCTGCATGCT GTGTCTCATG CAAAACCACA TGGTCCAGGC CTTTGCCAAC 
AGCGGCAATG CTATCAAGCC CGTCTCCTTC ATCAGAGACC TGAAAAAGAT TGCCCGGCAC 
TTCCGGTTCG GGAACCAAGA GGATGCACAC GAATTCCTGC GGTACACCAT TGATGCGATG 
CAGAAGGCCT GCTTGAATGG CTACGCTAAG TTGGATCGGC AGACACAGGC TACTACCCTG 
GTGCATCAGA TCTTTGGAGG CTATCTCAGG TCCCGAGTGA AGTGCTCTGT GTGCAAGAGT 
GTCTCAGACA CATACGATCC CTACTTGGAC ATAGCACTGG AGATCCGGCA AGCTGCAAAT 
ATTGTGCGCG CTCTGGAACT TTTTGTGAAG TCAGATGTCC TGAGTGGAGA GAACGCCTAT 
ATGTGTGCTA AGTGTAAGAA GAAGGTTCCA GCCAGCAAGC GCTTCACCAT CCATAGAACA 
TCCAATGTCC TGACTCTGTC CCTCAAGCGC TTCGCCAACT TTAGTGGGGG GAAGATCACC 
AAGGATGTTG GCTATCCAGA GTTCCTGAAC ATCCGTCCAT ACATGTCGCA GAGCAGCGGT 
GATCCTGTCA TGTATGGGCT CTATGCTGTC CTGGTGCACT CAGGCTACAG CTGTCACGCT 
GGGCACTACT ACTGCTATGT GAAGGCCAGC AATGGACAGT GGTACCAGAT GAACGACTCC 
TTGGTCCATT CCAGCAATGT CAAGGTGGTT CTGAACCAGC AGGCCTACGT GCTCTTCTAT 
CTGCGGATTC CAGGCTCTAA GAAAAGTCCT GAGGGCCCTG TCTCCAGAGT GGGCGCCACT 
CTCCCTAGCC GTCCCAAAGT TGTTCCAGAG CACTCCAAGA AGAGCCCTGG CAATGGGGTT 
GTTCCCTCCC CGCTGATGGC AAAGCGACAA GACTCTGTAA TGATGAGAAA GCTCCCAGCC 
CCTGAGGAGG TTGGCGTACC TGTGTCCAGA AATGGCTCCC TTCCTGGCCT GAAGTTACAG 
AACGGATGTG CTCCTGCCAA GACACCTGCC GGGTCCCCGT CCCCAAGACT CACGCCCACG 
CCCACTCATA TGCCCACCAT TCTGGATGAG CCCGGAAAGA AGGTGAAGAA GTCAGCCCCA 
CTGCAGTCTC TCACCACATC TCCAACCACT TCTCAGGGCT CTCCTGGGAC CGGCGAGTCC 
CGGAGCCAGC GGCCCGGCTC CTGGGCAAGC AGAGACACCA TCTTCTCTAC CTCCCCGAAG 
CTCCTGGCCA GAGCCATTAC TAACGGGCAC AGGCTGAAGG GCGAAGGCAG TGGTGTGGAC 
CTGGAGAAGG GGGATTCAAG CAGCTCCAGC CCCGAGCACT CTGCCAGCAG TGACCCTGCC 
AAGGCCCCGC AGACCGCAGA GAGCAGAGCT GCGCATGCCT GTGATTCTCA GGGAACAAAC 
TGTCCCACCG CTGGCCATCC CAAAGCGCTG TTGAATGGAG TAGATGCCAA GATGGTGAAG 
TTGAAGTCCC CGGCCCTGAG CAGCACCACA ACCGAGCCCA CAAGCCTCAT GTCTCCTCCA 
CCAGCCAAAA AACTGGCCCT GTCAGCCAAG AAGGCCAGCA CCCTGCGGAG GGCGACCGGC 
AATGACATCG GTTCACCTTC CCCCTCAGCA TTCTGCGACC TCACCTCCCC CATGAAAGCC 
ACCCACCCCG TCGTTGCCTC CACTGGGCCT GTCAGTAAGA CCAGGACTGC TGCACCTGCC 
CCCCGACCGT CCACCCACCC CCACTCTGCA TCTCTGTCCA GTAGTAGTGC CAAGCCCTTA 
GGGACATCAG AGCCACAGAG CTGCCGCCCC TCTGCCTGGA CACCCCTGCC TCAGGTCAAT 
GGACACTTCA CGTCCCACTT ACACCAACTG CCAGAGGCCA GCGAGGCCCT ACACAGCCCC 
TCCAAAAAGA GGAAAAAGAC CCCTAATGGA GATCCCCAGA GACTGGGCAT CGACACGCTC 
CTCCCACAGT GCCTCAGGGG AGCACCTGCA GCAGCACGCA GGAAGAGAAA GAAGAGGTGC 
TCGGAGGGTG AGGGTGCCAC AGCTCCCAAG CAGGAAGGCC AATTTCAGGA CCAGTCTTGG 
AGTTCCGGGA GCCAGAAGGA AGAGGGTACG CAGCCCCAGG TAAATGGCCA TCAAGTGAGT 
CACATTCTGG ACAGTTACCA TGTGAGCAGC AGGAAAAGGA GGAAGAGGAA AAGATCGGAA 
GGACTCAGCC AGGAAGCCAC CCCGTCGCAG GACCTAATTC AGCACAGCTG CTCCCCTGTA 
GACCACAGTG AGCCTGAGGC CAGGACAGAG TTGCAGAAGA AGAAGAAGAA GAAAAGAAGG 
AAAAGAAAGC CCGAGCCACA GCAGGATGAA GAGAGCAAGC ACCCAGGAGA CCAGAGGAGC 
CCAAGGCCCA GTGTCACCCC AGTCCCTGCG TTGAGTGTGA ATGGCCATCT TCCTAGTGAC 
TGCTTGGGGC TGGGACAAGC ACCTCTCGTT ACCTGGAACA GAGATCAGGA ACCCGATGTG 
GTCCAGGCGT TGCTTCAGGA CTCATCTGAT AAGGCTTATG GGAAAAAAGT TTTGACCTGG 
GATGGCGAGC CTTCAGCCAT CAGTCAGGAC GCCATCAAAG ACAGCAGACT AGCCCGGACC 
CAGACCGTGG TTGACGACTG GGATGAAGAG TTTGATCGAG GGAAGGAAAA GAAAATTAAA 
AAGTTCAAGA GAGAAAAGAA GAGAAACTTC AATGCCTTCC AGAAGCTTCA GAGTAGGCGC 
AACTTTTGGT CTGTGACGCA TCCTGCTAAG GTGGCCAGCC TCAGCTATCG CCGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Usp36 ( XM_006534303.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006534366.1 Mus musculus ubiquitin specific peptidase 36 (Usp36), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu12714
Clone ID Related Accession (Same CDS sequence)	XM_006534304.3 , NM_001033528.1 , XM_006534304.4 , XM_006534302.3 , XM_006534302.4 , XM_006534303.2
Accession Version	XM_006534303.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3297bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	ubiquitin carboxyl-terminal hydrolase 36 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 9, 2015 this sequence version replaced XM_006534303.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTATAG TCGATAAGCT GAAGGAGGCT CTCAAGCCTG GCCGCAAGGA CTCGGCCGAG 
GATGGGGACC TGGGCAGGCT GCTGGCAGCC TCTGCCAAGA AGGTGCTTCT GCAGAGGATT 
GAGTTTGAGC CGGCCAGCAA GAGCTTCTCC TACCAGCTGG AGTCCTTGAA GAGCAAATAC 
GTACTGTTGA GCGCCAGGGC TGAGGGAGCC AGTCGCCACA GGAGCGGAGA TGAACTCCAG 
GCCAGGAAAC CAGGCACCGA GCGTGTGTCT GGAAGTGGAG GCGACGGAGT CCCTGCCCCG 
CAGAAAGTGC TCTTCCCTGT GGAGCGTCTG TCCCTGAGGT GGGAACGTGT TTTCCGAGTT 
GGCGCAGGGC TGCACAACCT GGGTAACACC TGCTTCCTCA ACTCCACCAT CCAGTGCTTG 
ACCTACACAC CACCTCTGGC CAACTACTTG CTCTCCAAGG AGCACGCACG GAGCTGTCAC 
CAAGGGGGCT TCTGCATGCT GTGTCTCATG CAAAACCACA TGGTCCAGGC CTTTGCCAAC 
AGCGGCAATG CTATCAAGCC CGTCTCCTTC ATCAGAGACC TGAAAAAGAT TGCCCGGCAC 
TTCCGGTTCG GGAACCAAGA GGATGCACAC GAATTCCTGC GGTACACCAT TGATGCGATG 
CAGAAGGCCT GCTTGAATGG CTACGCTAAG TTGGATCGGC AGACACAGGC TACTACCCTG 
GTGCATCAGA TCTTTGGAGG CTATCTCAGG TCCCGAGTGA AGTGCTCTGT GTGCAAGAGT 
GTCTCAGACA CATACGATCC CTACTTGGAC ATAGCACTGG AGATCCGGCA AGCTGCAAAT 
ATTGTGCGCG CTCTGGAACT TTTTGTGAAG TCAGATGTCC TGAGTGGAGA GAACGCCTAT 
ATGTGTGCTA AGTGTAAGAA GAAGGTTCCA GCCAGCAAGC GCTTCACCAT CCATAGAACA 
TCCAATGTCC TGACTCTGTC CCTCAAGCGC TTCGCCAACT TTAGTGGGGG GAAGATCACC 
AAGGATGTTG GCTATCCAGA GTTCCTGAAC ATCCGTCCAT ACATGTCGCA GAGCAGCGGT 
GATCCTGTCA TGTATGGGCT CTATGCTGTC CTGGTGCACT CAGGCTACAG CTGTCACGCT 
GGGCACTACT ACTGCTATGT GAAGGCCAGC AATGGACAGT GGTACCAGAT GAACGACTCC 
TTGGTCCATT CCAGCAATGT CAAGGTGGTT CTGAACCAGC AGGCCTACGT GCTCTTCTAT 
CTGCGGATTC CAGGCTCTAA GAAAAGTCCT GAGGGCCCTG TCTCCAGAGT GGGCGCCACT 
CTCCCTAGCC GTCCCAAAGT TGTTCCAGAG CACTCCAAGA AGAGCCCTGG CAATGGGGTT 
GTTCCCTCCC CGCTGATGGC AAAGCGACAA GACTCTGTAA TGATGAGAAA GCTCCCAGCC 
CCTGAGGAGG TTGGCGTACC TGTGTCCAGA AATGGCTCCC TTCCTGGCCT GAAGTTACAG 
AACGGATGTG CTCCTGCCAA GACACCTGCC GGGTCCCCGT CCCCAAGACT CACGCCCACG 
CCCACTCATA TGCCCACCAT TCTGGATGAG CCCGGAAAGA AGGTGAAGAA GTCAGCCCCA 
CTGCAGTCTC TCACCACATC TCCAACCACT TCTCAGGGCT CTCCTGGGAC CGGCGAGTCC 
CGGAGCCAGC GGCCCGGCTC CTGGGCAAGC AGAGACACCA TCTTCTCTAC CTCCCCGAAG 
CTCCTGGCCA GAGCCATTAC TAACGGGCAC AGGCTGAAGG GCGAAGGCAG TGGTGTGGAC 
CTGGAGAAGG GGGATTCAAG CAGCTCCAGC CCCGAGCACT CTGCCAGCAG TGACCCTGCC 
AAGGCCCCGC AGACCGCAGA GAGCAGAGCT GCGCATGCCT GTGATTCTCA GGGAACAAAC 
TGTCCCACCG CTGGCCATCC CAAAGCGCTG TTGAATGGAG TAGATGCCAA GATGGTGAAG 
TTGAAGTCCC CGGCCCTGAG CAGCACCACA ACCGAGCCCA CAAGCCTCAT GTCTCCTCCA 
CCAGCCAAAA AACTGGCCCT GTCAGCCAAG AAGGCCAGCA CCCTGCGGAG GGCGACCGGC 
AATGACATCG GTTCACCTTC CCCCTCAGCA TTCTGCGACC TCACCTCCCC CATGAAAGCC 
ACCCACCCCG TCGTTGCCTC CACTGGGCCT GTCAGTAAGA CCAGGACTGC TGCACCTGCC 
CCCCGACCGT CCACCCACCC CCACTCTGCA TCTCTGTCCA GTAGTAGTGC CAAGCCCTTA 
GGGACATCAG AGCCACAGAG CTGCCGCCCC TCTGCCTGGA CACCCCTGCC TCAGGTCAAT 
GGACACTTCA CGTCCCACTT ACACCAACTG CCAGAGGCCA GCGAGGCCCT ACACAGCCCC 
TCCAAAAAGA GGAAAAAGAC CCCTAATGGA GATCCCCAGA GACTGGGCAT CGACACGCTC 
CTCCCACAGT GCCTCAGGGG AGCACCTGCA GCAGCACGCA GGAAGAGAAA GAAGAGGTGC 
TCGGAGGGTG AGGGTGCCAC AGCTCCCAAG CAGGAAGGCC AATTTCAGGA CCAGTCTTGG 
AGTTCCGGGA GCCAGAAGGA AGAGGGTACG CAGCCCCAGG TAAATGGCCA TCAAGTGAGT 
CACATTCTGG ACAGTTACCA TGTGAGCAGC AGGAAAAGGA GGAAGAGGAA AAGATCGGAA 
GGACTCAGCC AGGAAGCCAC CCCGTCGCAG GACCTAATTC AGCACAGCTG CTCCCCTGTA 
GACCACAGTG AGCCTGAGGC CAGGACAGAG TTGCAGAAGA AGAAGAAGAA GAAAAGAAGG 
AAAAGAAAGC CCGAGCCACA GCAGGATGAA GAGAGCAAGC ACCCAGGAGA CCAGAGGAGC 
CCAAGGCCCA GTGTCACCCC AGTCCCTGCG TTGAGTGTGA ATGGCCATCT TCCTAGTGAC 
TGCTTGGGGC TGGGACAAGC ACCTCTCGTT ACCTGGAACA GAGATCAGGA ACCCGATGTG 
GTCCAGGCGT TGCTTCAGGA CTCATCTGAT AAGGCTTATG GGAAAAAAGT TTTGACCTGG 
GATGGCGAGC CTTCAGCCAT CAGTCAGGAC GCCATCAAAG ACAGCAGACT AGCCCGGACC 
CAGACCGTGG TTGACGACTG GGATGAAGAG TTTGATCGAG GGAAGGAAAA GAAAATTAAA 
AAGTTCAAGA GAGAAAAGAA GAGAAACTTC AATGCCTTCC AGAAGCTTCA GAGTAGGCGC 
AACTTTTGGT CTGTGACGCA TCCTGCTAAG GTGGCCAGCC TCAGCTATCG CCGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006534366.1
CDS	568..3864
Translation	MPIVDKLKEALKPGRKDSAEDGDLGRLLAASAKKVLLQRIEFEPASKSFSYQLESLKSKYVLLSARAEGASRHRSGDELQARKPGTERVSGSGGDGVPAPQKVLFPVERLSLRWERVFRVGAGLHNLGNTCFLNSTIQCLTYTPPLANYLLSKEHARSCHQGGFCMLCLMQNHMVQAFANSGNAIKPVSFIRDLKKIARHFRFGNQEDAHEFLRYTIDAMQKACLNGYAKLDRQTQATTLVHQIFGGYLRSRVKCSVCKSVSDTYDPYLDIALEIRQAANIVRALELFVKSDVLSGENAYMCAKCKKKVPASKRFTIHRTSNVLTLSLKRFANFSGGKITKDVGYPEFLNIRPYMSQSSGDPVMYGLYAVLVHSGYSCHAGHYYCYVKASNGQWYQMNDSLVHSSNVKVVLNQQAYVLFYLRIPGSKKSPEGPVSRVGATLPSRPKVVPEHSKKSPGNGVVPSPLMAKRQDSVMMRKLPAPEEVGVPVSRNGSLPGLKLQNGCAPAKTPAGSPSPRLTPTPTHMPTILDEPGKKVKKSAPLQSLTTSPTTSQGSPGTGESRSQRPGSWASRDTIFSTSPKLLARAITNGHRLKGEGSGVDLEKGDSSSSSPEHSASSDPAKAPQTAESRAAHACDSQGTNCPTAGHPKALLNGVDAKMVKLKSPALSSTTTEPTSLMSPPPAKKLALSAKKASTLRRATGNDIGSPSPSAFCDLTSPMKATHPVVASTGPVSKTRTAAPAPRPSTHPHSASLSSSSAKPLGTSEPQSCRPSAWTPLPQVNGHFTSHLHQLPEASEALHSPSKKRKKTPNGDPQRLGIDTLLPQCLRGAPAAARRKRKKRCSEGEGATAPKQEGQFQDQSWSSGSQKEEGTQPQVNGHQVSHILDSYHVSSRKRRKRKRSEGLSQEATPSQDLIQHSCSPVDHSEPEARTELQKKKKKKRRKRKPEPQQDEESKHPGDQRSPRPSVTPVPALSVNGHLPSDCLGLGQAPLVTWNRDQEPDVVQALLQDSSDKAYGKKVLTWDGEPSAISQDAIKDSRLARTQTVVDDWDEEFDRGKEKKIKKFKREKKRNFNAFQKLQSRRNFWSVTHPAKVASLSYRR

Target ORF information:

RefSeq Version	XM_006534303.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus ubiquitin specific peptidase 36 (Usp36), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006534303.2

ORF Insert Sequence:

ATGCCTATAG TCGATAAGCT GAAGGAGGCT CTCAAGCCTG GCCGCAAGGA CTCGGCCGAG 
GATGGGGACC TGGGCAGGCT GCTGGCAGCC TCTGCCAAGA AGGTGCTTCT GCAGAGGATT 
GAGTTTGAGC CGGCCAGCAA GAGCTTCTCC TACCAGCTGG AGTCCTTGAA GAGCAAATAC 
GTACTGTTGA GCGCCAGGGC TGAGGGAGCC AGTCGCCACA GGAGCGGAGA TGAACTCCAG 
GCCAGGAAAC CAGGCACCGA GCGTGTGTCT GGAAGTGGAG GCGACGGAGT CCCTGCCCCG 
CAGAAAGTGC TCTTCCCTGT GGAGCGTCTG TCCCTGAGGT GGGAACGTGT TTTCCGAGTT 
GGCGCAGGGC TGCACAACCT GGGTAACACC TGCTTCCTCA ACTCCACCAT CCAGTGCTTG 
ACCTACACAC CACCTCTGGC CAACTACTTG CTCTCCAAGG AGCACGCACG GAGCTGTCAC 
CAAGGGGGCT TCTGCATGCT GTGTCTCATG CAAAACCACA TGGTCCAGGC CTTTGCCAAC 
AGCGGCAATG CTATCAAGCC CGTCTCCTTC ATCAGAGACC TGAAAAAGAT TGCCCGGCAC 
TTCCGGTTCG GGAACCAAGA GGATGCACAC GAATTCCTGC GGTACACCAT TGATGCGATG 
CAGAAGGCCT GCTTGAATGG CTACGCTAAG TTGGATCGGC AGACACAGGC TACTACCCTG 
GTGCATCAGA TCTTTGGAGG CTATCTCAGG TCCCGAGTGA AGTGCTCTGT GTGCAAGAGT 
GTCTCAGACA CATACGATCC CTACTTGGAC ATAGCACTGG AGATCCGGCA AGCTGCAAAT 
ATTGTGCGCG CTCTGGAACT TTTTGTGAAG TCAGATGTCC TGAGTGGAGA GAACGCCTAT 
ATGTGTGCTA AGTGTAAGAA GAAGGTTCCA GCCAGCAAGC GCTTCACCAT CCATAGAACA 
TCCAATGTCC TGACTCTGTC CCTCAAGCGC TTCGCCAACT TTAGTGGGGG GAAGATCACC 
AAGGATGTTG GCTATCCAGA GTTCCTGAAC ATCCGTCCAT ACATGTCGCA GAGCAGCGGT 
GATCCTGTCA TGTATGGGCT CTATGCTGTC CTGGTGCACT CAGGCTACAG CTGTCACGCT 
GGGCACTACT ACTGCTATGT GAAGGCCAGC AATGGACAGT GGTACCAGAT GAACGACTCC 
TTGGTCCATT CCAGCAATGT CAAGGTGGTT CTGAACCAGC AGGCCTACGT GCTCTTCTAT 
CTGCGGATTC CAGGCTCTAA GAAAAGTCCT GAGGGCCCTG TCTCCAGAGT GGGCGCCACT 
CTCCCTAGCC GTCCCAAAGT TGTTCCAGAG CACTCCAAGA AGAGCCCTGG CAATGGGGTT 
GTTCCCTCCC CGCTGATGGC AAAGCGACAA GACTCTGTAA TGATGAGAAA GCTCCCAGCC 
CCTGAGGAGG TTGGCGTACC TGTGTCCAGA AATGGCTCCC TTCCTGGCCT GAAGTTACAG 
AACGGATGTG CTCCTGCCAA GACACCTGCC GGGTCCCCGT CCCCAAGACT CACGCCCACG 
CCCACTCATA TGCCCACCAT TCTGGATGAG CCCGGAAAGA AGGTGAAGAA GTCAGCCCCA 
CTGCAGTCTC TCACCACATC TCCAACCACT TCTCAGGGCT CTCCTGGGAC CGGCGAGTCC 
CGGAGCCAGC GGCCCGGCTC CTGGGCAAGC AGAGACACCA TCTTCTCTAC CTCCCCGAAG 
CTCCTGGCCA GAGCCATTAC TAACGGGCAC AGGCTGAAGG GCGAAGGCAG TGGTGTGGAC 
CTGGAGAAGG GGGATTCAAG CAGCTCCAGC CCCGAGCACT CTGCCAGCAG TGACCCTGCC 
AAGGCCCCGC AGACCGCAGA GAGCAGAGCT GCGCATGCCT GTGATTCTCA GGGAACAAAC 
TGTCCCACCG CTGGCCATCC CAAAGCGCTG TTGAATGGAG TAGATGCCAA GATGGTGAAG 
TTGAAGTCCC CGGCCCTGAG CAGCACCACA ACCGAGCCCA CAAGCCTCAT GTCTCCTCCA 
CCAGCCAAAA AACTGGCCCT GTCAGCCAAG AAGGCCAGCA CCCTGCGGAG GGCGACCGGC 
AATGACATCG GTTCACCTTC CCCCTCAGCA TTCTGCGACC TCACCTCCCC CATGAAAGCC 
ACCCACCCCG TCGTTGCCTC CACTGGGCCT GTCAGTAAGA CCAGGACTGC TGCACCTGCC 
CCCCGACCGT CCACCCACCC CCACTCTGCA TCTCTGTCCA GTAGTAGTGC CAAGCCCTTA 
GGGACATCAG AGCCACAGAG CTGCCGCCCC TCTGCCTGGA CACCCCTGCC TCAGGTCAAT 
GGACACTTCA CGTCCCACTT ACACCAACTG CCAGAGGCCA GCGAGGCCCT ACACAGCCCC 
TCCAAAAAGA GGAAAAAGAC CCCTAATGGA GATCCCCAGA GACTGGGCAT CGACACGCTC 
CTCCCACAGT GCCTCAGGGG AGCACCTGCA GCAGCACGCA GGAAGAGAAA GAAGAGGTGC 
TCGGAGGGTG AGGGTGCCAC AGCTCCCAAG CAGGAAGGCC AATTTCAGGA CCAGTCTTGG 
AGTTCCGGGA GCCAGAAGGA AGAGGGTACG CAGCCCCAGG TAAATGGCCA TCAAGTGAGT 
CACATTCTGG ACAGTTACCA TGTGAGCAGC AGGAAAAGGA GGAAGAGGAA AAGATCGGAA 
GGACTCAGCC AGGAAGCCAC CCCGTCGCAG GACCTAATTC AGCACAGCTG CTCCCCTGTA 
GACCACAGTG AGCCTGAGGC CAGGACAGAG TTGCAGAAGA AGAAGAAGAA GAAAAGAAGG 
AAAAGAAAGC CCGAGCCACA GCAGGATGAA GAGAGCAAGC ACCCAGGAGA CCAGAGGAGC 
CCAAGGCCCA GTGTCACCCC AGTCCCTGCG TTGAGTGTGA ATGGCCATCT TCCTAGTGAC 
TGCTTGGGGC TGGGACAAGC ACCTCTCGTT ACCTGGAACA GAGATCAGGA ACCCGATGTG 
GTCCAGGCGT TGCTTCAGGA CTCATCTGAT AAGGCTTATG GGAAAAAAGT TTTGACCTGG 
GATGGCGAGC CTTCAGCCAT CAGTCAGGAC GCCATCAAAG ACAGCAGACT AGCCCGGACC 
CAGACCGTGG TTGACGACTG GGATGAAGAG TTTGATCGAG GGAAGGAAAA GAAAATTAAA 
AAGTTCAAGA GAGAAAAGAA GAGAAACTTC AATGCCTTCC AGAAGCTTCA GAGTAGGCGC 
AACTTTTGGT CTGTGACGCA TCCTGCTAAG GTGGCCAGCC TCAGCTATCG CCGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Loss of the Deubiquitinase USP36 Destabilizes the RNA Helicase DHX33 and Causes Preimplantation Lethality in Mice.
The Journal of biological chemistry(2017 Dec)
Fraile JM,Campos-Iglesias D,Rodr?guez F,Astudillo A,Vilarrasa-Blasi R,Verdaguer-Dot N,Prado MA,Paulo JA,Gygi SP,Mart?n-Subero JI,Freije JMP,L?pez-Ot?n C

High-throughput discovery of novel developmental phenotypes.
Nature537(7621)508-514(2016 09)
Dickinson ME,Flenniken AM,Ji X,Teboul L,Wong MD,White JK,Meehan TF,Weninger WJ,Westerberg H,Adissu H,Baker CN,Bower L,Brown JM,Caddle LB,Chiani F,Clary D,Cleak J,Daly MJ,Denegre JM,Doe B,Dolan ME,Edie SM,Fuchs H,Gailus-Durner V,Galli A,Gambadoro A,Gallegos J,Guo S,Horner NR,Hsu CW,Johnson SJ,Kalaga S,Keith LC,Lanoue L,Lawson TN,Lek M,Mark M,Marschall S,Mason J,McElwee ML,Newbigging S,Nutter LM,Peterson KA,Ramirez-Solis R,Rowland DJ,Ryder E,Samocha KE,Seavitt JR,Selloum M,Szoke-Kovacs Z,Tamura M,Trainor AG,Tudose I,Wakana S,Warren J,Wendling O,West DB,Wong L,Yoshiki A,,,,,,,,,MacArthur DG,Tocchini-Valentini GP,Gao X,Flicek P,Bradley A,Skarnes WC,Justice MJ,Parkinson HE,Moore M,Wells S,Braun RE,Svenson KL,de Angelis MH,Herault Y,Mohun T,Mallon AM,Henkelman RM,Brown SD,Adams DJ,Lloyd KC,McKerlie C,Beaudet AL,Bu?an M,Murray SA

Ubiquitin-specific Protease 36 (USP36) Controls Neuronal Precursor Cell-expressed Developmentally Down-regulated 4-2 (Nedd4-2) Actions over the Neurotrophin Receptor TrkA and Potassium Voltage-gated Channels 7.2/3 (Kv7.2/3).
The Journal of biological chemistry291(36)19132-45(2016 09)
Anta B,Mart?n-Rodr?guez C,Gomis-Perez C,Calvo L,L?pez-Benito S,Calder?n-Garc?a AA,Vicente-Garc?a C,Villarroel ?,Ar?valo JC

Prediction of the coding sequences of mouse homologues of KIAA gene: III. the complete nucleotide sequences of 500 mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries.
DNA research : an international journal for rapid publication of reports on genes and genomes10(4)167-80(2003 Aug)
Okazaki N,Kikuno R,Ohara R,Inamoto S,Koseki H,Hiraoka S,Saga Y,Nagase T,Ohara O,Koga H

BayGenomics: a resource of insertional mutations in mouse embryonic stem cells.
Nucleic acids research31(1)278-81(2003 Jan)
Stryke D,Kawamoto M,Huang CC,Johns SJ,King LA,Harper CA,Meng EC,Lee RE,Yee A,L'Italien L,Chuang PT,Young SG,Skarnes WC,Babbitt PC,Ferrin TE

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Usp36 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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