Pacrg cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Pacrg
Entrez Gene ID	69310
Full Name	PARK2 co-regulated
Synonyms	1700008H23Rik
General protein information	Preferred Names PARK2 co-regulated Names parkin coregulated gene protein homolog PARK2 coregulated gene protein hypertension-related protein 1-like protein parkin co-regulated gene protein
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 17 Map Location: 17 A1\|17 7.8 cM

mRNA and Protein(s)

mRNA	Protein	Name
XM_006523353.3	XP_006523416.1	parkin coregulated gene protein homolog isoform X1
XM_006523353.4	XP_006523416.1	parkin coregulated gene protein homolog isoform X1
NM_027032.2	NP_081308.1	parkin coregulated gene protein homolog

Pathways from BioSystems

Homologies

Bos taurus (cattle)	PACRG	XP_002690427.3
Drosophila melanogaster (fruit fly)	CG15120	NP_611428.1
Xenopus tropicalis (tropical clawed frog)	LOC100127543	NP_001106393.1
Canis lupus familiaris (dog)	PACRG	XP_541188.2
Pan troglodytes (chimpanzee)	PACRG	XP_001154274.1
Gallus gallus (chicken)	PACRG	XP_419614.2
Danio rerio (zebrafish)	pacrg	NP_001007399.1
Mus musculus (house mouse)	Pacrg	NP_081308.1
Rattus norvegicus (Norway rat)	LOC100912128	XP_003748744.1
Homo sapiens (human)	PACRG	NP_689623.2
	PACRG	XP_001100964.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP011313	XP_309337.3

Gene Ontology
Bibliography

Related articles in PubMed

A MEIG1/PACRG complex in the manchette is essential for building the sperm flagella.
Li W, Tang W, Teves ME, Zhang Z, Zhang L, Li H, Archer KJ, Peterson DL, Williams DC Jr, Strauss JF 3rd, Zhang Z
Development (Cambridge, England)142(5)921-30(2015 Mar)

Quantitative trait loci analysis reveals candidate genes implicated in regulating functional deficit and CNS vascular permeability in CD8 T cell-initiated blood-brain barrier disruption.
Johnson HL, Hanson LM, Chen Y, Bieber AJ, Buono RJ, Ferraro TN, Pirko I, Johnson AJ
BMC genomics14678(2013 Oct)

Ciliary and non-ciliary expression and function of PACRG during vertebrate development.
Thumberger T, Hagenlocher C, Tisler M, Beyer T, Tietze N, Schweickert A, Feistel K, Blum M
Cilia1(1)13(2012 Aug)

Deletion of the Parkin co-regulated gene causes defects in ependymal ciliary motility and hydrocephalus in the quakingviable mutant mouse.
Wilson GR, Wang HX, Egan GF, Robinson PJ, Delatycki MB, O'Bryan MK, Lockhart PJ
Human molecular genetics19(8)1593-602(2010 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Studies demonstrate that MEIG1/PACRG forms a complex in the manchette and that this complex is necessary to transport cargos, such as SPAG16L, to build the sperm flagella.
Title: A MEIG1/PACRG complex in the manchette is essential for building the sperm flagella.

Pacrg is required for motile cilia function and may be involved in the pathogenesis of hydrocephalus.
Title: Deletion of the Parkin co-regulated gene causes defects in ependymal ciliary motility and hydrocephalus in the quakingviable mutant mouse.

Findings reveal a critical role for the MEIG1/PARCG partnership in manchette structure and function and the control of spermiogenesis.
Title: MEIG1 is essential for spermiogenesis in mice.

The results of this study indicated that expression of Pacrg in the cells associated with the cerebral ventricle, in addition to the aqueduct of Sylvius and choroid plexus. And strong expression was observed since newborn period to adulthood.
Title: Expression and localization of the Parkin co-regulated gene in mouse CNS suggests a role in ependymal cilia function.

These results suggest that the transcriptional co-regulation of Pacrg and parkin leads to a similar regional protein distribution in mouse brain, which may have functional significance for the two proteins.
Title: Regional and cellular localisation of Parkin co-regulated gene in developing and adult mouse brain.

The following Pacrg gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Pacrg cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu03461	NM_027032.2 Latest version!	Mus musculus PARK2 co-regulated (Pacrg), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$139.30 $199.00
OMu52924	XM_006523353.4 Latest version!	Mus musculus PARK2 co-regulated (Pacrg), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$69.30 $99.00
View Old Accession Versions >>
OMu52924	XM_006523353.3	Mus musculus PARK2 co-regulated (Pacrg), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$69.30 $99.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Pacrg ( XM_006523353.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006523416.1 Mus musculus PARK2 co-regulated (Pacrg), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu52924
Clone ID Related Accession (Same CDS sequence)	XM_006523353.3 , XM_006523353.4
Accession Version	XM_006523353.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 420bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	parkin coregulated gene protein homolog isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000083.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006523353.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGTTTC CCTATGAGTT TTTTGCTCGG CGAGGAATCC ACGACATGCT GGAACACGGA 
GGGAACAAGA TTCTACCTGT CATTCCTCAG CTCATTATCC CAATAAAAAA TGCCTTGAAC 
CTCCGAAACA GACAGATCAT CTGTGTGACG CTCAAGGTTC TCCAGCATCT GGTTGTGTCT 
TCGGAGATGG TGGGCGAAGC TTTGTTGCCC TACTACCGTC AGATCCTGCC AATCCTGAAC 
ATCTTTAAGA ACATGAATGT GAACTCCGGG GATGGCATCG ACTACAGCCA GCAGAAGAGG 
GAGAACATTG GCGACCTGAT CCAGGAGACC CTAGAAGCCT TCGAGCGCTA CGGAGGGGAG 
GACGCCTTCA TCAACATCAA ATACATGGTG CCTACCTATG AGTCGTGCTT GCTGAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006523416.1
CDS	279..698
Translation	MTFPYEFFARRGIHDMLEHGGNKILPVIPQLIIPIKNALNLRNRQIICVTLKVLQHLVVSSEMVGEALLPYYRQILPILNIFKNMNVNSGDGIDYSQQKRENIGDLIQETLEAFERYGGEDAFINIKYMVPTYESCLLN

Target ORF information:

RefSeq Version	XM_006523353.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus PARK2 co-regulated (Pacrg), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006523353.3

ORF Insert Sequence:

ATGACGTTTC CCTATGAGTT TTTTGCTCGG CGAGGAATCC ACGACATGCT GGAACACGGA 
GGGAACAAGA TTCTACCTGT CATTCCTCAG CTCATTATCC CAATAAAAAA TGCCTTGAAC 
CTCCGAAACA GACAGATCAT CTGTGTGACG CTCAAGGTTC TCCAGCATCT GGTTGTGTCT 
TCGGAGATGG TGGGCGAAGC TTTGTTGCCC TACTACCGTC AGATCCTGCC AATCCTGAAC 
ATCTTTAAGA ACATGAATGT GAACTCCGGG GATGGCATCG ACTACAGCCA GCAGAAGAGG 
GAGAACATTG GCGACCTGAT CCAGGAGACC CTAGAAGCCT TCGAGCGCTA CGGAGGGGAG 
GACGCCTTCA TCAACATCAA ATACATGGTG CCTACCTATG AGTCGTGCTT GCTGAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pacrg ( NM_027032.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_081308.1 Mus musculus PARK2 co-regulated (Pacrg), mRNA.

Price & Availability↑

CloneID	OMu03461
Clone ID Related Accession (Same CDS sequence)	NM_027032.2
Accession Version	NM_027032.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 726bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-13
Organism	Mus musculus(house mouse)
Product	parkin coregulated gene protein homolog
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK135510.1 and AK005771.1. On Nov 10, 2006 this sequence version replaced NM_027032.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK135510.1, AK005771.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164138 [ECO:0000348] ##Evidence-Data-END##

ATGCCGAAGA GGACTAAACT GCTGCCACAA CAGACGTTCC AGGTGCACCA GCCTCGTTCC 
CTGGTTTCTG AGGGCTTCAC AGTCAAAGCC ATGATGAAAA ACTCAGTCGT GCGGGGTCCT 
CCAGTTGCAG GGGCATTTAA AGAAAGGCCG GCCAAACCCA CCACTTTCCG AAAATGTTAT 
GAGCGAGGAG ACTTCCCAAT CGCCCTTGAG CATGACTCGA AAGGAAACAA AATTGCCTGG 
AAGGTTGAGA TTGAGAAGTT GGACTACCAT CATTACCTGC CTCTGTTTTT TGATGGGCTT 
TCTGAAATGA CGTTTCCCTA TGAGTTTTTT GCTCGGCGAG GAATCCACGA CATGCTGGAA 
CACGGAGGGA ACAAGATTCT ACCTGTCATT CCTCAGCTCA TTATCCCAAT AAAAAATGCC 
TTGAACCTCC GAAACAGACA GATCATCTGT GTGACGCTCA AGGTTCTCCA GCATCTGGTT 
GTGTCTTCGG AGATGGTGGG CGAAGCTTTG TTGCCCTACT ACCGTCAGAT CCTGCCAATC 
CTGAACATCT TTAAGAACAT GAATGTGAAC TCCGGGGATG GCATCGACTA CAGCCAGCAG 
AAGAGGGAGA ACATTGGCGA CCTGATCCAG GAGACCCTAG AAGCCTTCGA GCGCTACGGA 
GGGGAGGACG CCTTCATCAA CATCAAATAC ATGGTGCCTA CCTATGAGTC GTGCTTGCTG 
AACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_081308.1
CDS	324..1049
Translation	MPKRTKLLPQQTFQVHQPRSLVSEGFTVKAMMKNSVVRGPPVAGAFKERPAKPTTFRKCYERGDFPIALEHDSKGNKIAWKVEIEKLDYHHYLPLFFDGLSEMTFPYEFFARRGIHDMLEHGGNKILPVIPQLIIPIKNALNLRNRQIICVTLKVLQHLVVSSEMVGEALLPYYRQILPILNIFKNMNVNSGDGIDYSQQKRENIGDLIQETLEAFERYGGEDAFINIKYMVPTYESCLLN

Target ORF information:

RefSeq Version	NM_027032.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus PARK2 co-regulated (Pacrg), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_027032.2

ORF Insert Sequence:

ATGCCGAAGA GGACTAAACT GCTGCCACAA CAGACGTTCC AGGTGCACCA GCCTCGTTCC 
CTGGTTTCTG AGGGCTTCAC AGTCAAAGCC ATGATGAAAA ACTCAGTCGT GCGGGGTCCT 
CCAGTTGCAG GGGCATTTAA AGAAAGGCCG GCCAAACCCA CCACTTTCCG AAAATGTTAT 
GAGCGAGGAG ACTTCCCAAT CGCCCTTGAG CATGACTCGA AAGGAAACAA AATTGCCTGG 
AAGGTTGAGA TTGAGAAGTT GGACTACCAT CATTACCTGC CTCTGTTTTT TGATGGGCTT 
TCTGAAATGA CGTTTCCCTA TGAGTTTTTT GCTCGGCGAG GAATCCACGA CATGCTGGAA 
CACGGAGGGA ACAAGATTCT ACCTGTCATT CCTCAGCTCA TTATCCCAAT AAAAAATGCC 
TTGAACCTCC GAAACAGACA GATCATCTGT GTGACGCTCA AGGTTCTCCA GCATCTGGTT 
GTGTCTTCGG AGATGGTGGG CGAAGCTTTG TTGCCCTACT ACCGTCAGAT CCTGCCAATC 
CTGAACATCT TTAAGAACAT GAATGTGAAC TCCGGGGATG GCATCGACTA CAGCCAGCAG 
AAGAGGGAGA ACATTGGCGA CCTGATCCAG GAGACCCTAG AAGCCTTCGA GCGCTACGGA 
GGGGAGGACG CCTTCATCAA CATCAAATAC ATGGTGCCTA CCTATGAGTC GTGCTTGCTG 
AACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pacrg ( XM_006523353.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006523416.1 Mus musculus PARK2 co-regulated (Pacrg), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu52924
Clone ID Related Accession (Same CDS sequence)	XM_006523353.3 , XM_006523353.4
Accession Version	XM_006523353.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 420bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	parkin coregulated gene protein homolog isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000083.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006523353.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGTTTC CCTATGAGTT TTTTGCTCGG CGAGGAATCC ACGACATGCT GGAACACGGA 
GGGAACAAGA TTCTACCTGT CATTCCTCAG CTCATTATCC CAATAAAAAA TGCCTTGAAC 
CTCCGAAACA GACAGATCAT CTGTGTGACG CTCAAGGTTC TCCAGCATCT GGTTGTGTCT 
TCGGAGATGG TGGGCGAAGC TTTGTTGCCC TACTACCGTC AGATCCTGCC AATCCTGAAC 
ATCTTTAAGA ACATGAATGT GAACTCCGGG GATGGCATCG ACTACAGCCA GCAGAAGAGG 
GAGAACATTG GCGACCTGAT CCAGGAGACC CTAGAAGCCT TCGAGCGCTA CGGAGGGGAG 
GACGCCTTCA TCAACATCAA ATACATGGTG CCTACCTATG AGTCGTGCTT GCTGAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006523416.1
CDS	310..729
Translation	MTFPYEFFARRGIHDMLEHGGNKILPVIPQLIIPIKNALNLRNRQIICVTLKVLQHLVVSSEMVGEALLPYYRQILPILNIFKNMNVNSGDGIDYSQQKRENIGDLIQETLEAFERYGGEDAFINIKYMVPTYESCLLN

Target ORF information:

RefSeq Version	XM_006523353.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus PARK2 co-regulated (Pacrg), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006523353.4

ORF Insert Sequence:

ATGACGTTTC CCTATGAGTT TTTTGCTCGG CGAGGAATCC ACGACATGCT GGAACACGGA 
GGGAACAAGA TTCTACCTGT CATTCCTCAG CTCATTATCC CAATAAAAAA TGCCTTGAAC 
CTCCGAAACA GACAGATCAT CTGTGTGACG CTCAAGGTTC TCCAGCATCT GGTTGTGTCT 
TCGGAGATGG TGGGCGAAGC TTTGTTGCCC TACTACCGTC AGATCCTGCC AATCCTGAAC 
ATCTTTAAGA ACATGAATGT GAACTCCGGG GATGGCATCG ACTACAGCCA GCAGAAGAGG 
GAGAACATTG GCGACCTGAT CCAGGAGACC CTAGAAGCCT TCGAGCGCTA CGGAGGGGAG 
GACGCCTTCA TCAACATCAA ATACATGGTG CCTACCTATG AGTCGTGCTT GCTGAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A MEIG1/PACRG complex in the manchette is essential for building the sperm flagella.
Development (Cambridge, England)142(5)921-30(2015 Mar)
Li W,Tang W,Teves ME,Zhang Z,Zhang L,Li H,Archer KJ,Peterson DL,Williams DC Jr,Strauss JF 3rd,Zhang Z

Quantitative trait loci analysis reveals candidate genes implicated in regulating functional deficit and CNS vascular permeability in CD8 T cell-initiated blood-brain barrier disruption.
BMC genomics14678(2013 Oct)
Johnson HL,Hanson LM,Chen Y,Bieber AJ,Buono RJ,Ferraro TN,Pirko I,Johnson AJ

Ciliary and non-ciliary expression and function of PACRG during vertebrate development.
Cilia1(1)13(2012 Aug)
Thumberger T,Hagenlocher C,Tisler M,Beyer T,Tietze N,Schweickert A,Feistel K,Blum M

Deletion of the Parkin co-regulated gene causes defects in ependymal ciliary motility and hydrocephalus in the quakingviable mutant mouse.
Human molecular genetics19(8)1593-602(2010 Apr)
Wilson GR,Wang HX,Egan GF,Robinson PJ,Delatycki MB,O'Bryan MK,Lockhart PJ